Abstract: A method for sequencing a polynucleotide sample having a barcode sequence includes: introducing a series of nucleotides to the polynucleotide sample according to a predetermined order of nucleotide flows; obtaining a series of signals resulting from the introducing of nucleotides to the polynucleotide sample; and resolving the series of signals over the barcode sequence to render a flowspace string, wherein the flowspace string is a codeword of an error-correcting code that is (i) designed based on and adapted for use with the predetermined order of nucleotide flows, and (ii) capable of distinguishing any codeword in the error-correcting code from the other codewords in the error-correcting code in the presence of zero, one, and two errors.

Abstract: The disclosure provides systems and methods for detecting and analyzing the metabolome of a cell. Such methods and systems can include or make use of barcoding of nucleic acid molecules and their sequencing.

Abstract: Biomarkers for identifying sepsis in humans are presented herein, as are related methods, uses, agents, and kits comprising same. Methods for treating, detecting, and diagnosing sepsis in humans are presented herein.

Abstract: Methods and compositions for the detection of single nucleotide polymorphisms in a sample are provided. The methods and compositions are employed to determine whether the subject has autism spectrum disorder (ASD), is likely to develop ASD, or to classify a subject as having a particular ASD subtype. In one method of the invention, a sample is probed for one or more SNPs in Table 1, Table 2, Table 3, Table 6 or Table 7 at the nucleic acid level by performing a polymerase chain reaction (PCR) with primers specific to the SNPs. The presence and/or absence of the one or more SNPs is then compared to the presence and/or absence of the of the SNPs in at least one sample training set(s), where the comparing step comprises applying a statistical algorithm which comprises determining a correlation between the SNP data obtained from the sample and the SNP data from the at least one training set. The sample is diagnosed as ASD positive or ASD negative based on the results of the statistical algorithm.

Abstract: The present invention relates to the use of the editing profile of PDE8A pre-mRNA as a specific bio marker of ADARs activities in evolved primate, particularly in Human tissues. The present invention also relates to an in vitro method for predicting in Human an alteration of the mechanism of the ADARs catalysed pre-mRNA editing of target genes, by analysing the PDE8A pre-mRNA editing profile in a peripheral tissue sample containing cells expressing said PDE8A pre-mRNA, such as blood sample. The present invention is also directed to an in vitro method for the screening of potential therapeutic compound and to predict and assess therapeutic efficacy and/or efficiency or to diagnose potential severe brain or peripheral drug side effects implementing said PDE8A pre-mRNA editing profile as specific biomarker.

Abstract: Specific single nucleotide polymorphisms (SNPs) in the human genome, and their association with deep vein thrombosis (DVT) and related pathologies, such as pulmonary embolism (PE).

Abstract: Provided herein are methods and computer-readable storage media related to cell-free DNA and uses thereof to determine risk of a condition, such as transplant rejection or cancer, in a subject.

Abstract: Methods and kits for diagnosing or predicting the risk of developing psoriatic arthritis in a subject in need of such diagnosis or risk prediction are provided, comprising measuring the expression level of one or more miRNAs disclosed herein in the sample of the subject, and compared the expression level of at least one of the miRNAs in the test sample with that of the psoriatic arthritis-free sample. Also provided are methods of treating psoriatic arthritis by reducing the expression level of at least one of the miRNAs disclosed herein.

Abstract: The invention refers to a method of detecting one or more deoxyribose nucleotide (e.g. DNA) template(s) in a heterogenous sample by generation of template specific surrogate nucleotide sequences. This invention is applied to but not restricted in detecting genetic variations including cancer markers and pathogens.

Abstract: The present invention provides screening kits, compositions, and diagnostic methods for determining whether a subject has a predisposition to, or likelihood of having, a substance use disorder by determining a nucleic acid methylation profile from a biological sample from the subject, wherein a given profile indicates that the subject has a predisposition to a substance use disorder.

Abstract: Methods and nucleic acid molecules for detecting chromosomal abnormalities such as aneuploidy. Methods for selecting nucleic acid molecules for use in the methods of the disclosure.

Abstract: This disclosure provides methods for predicting the likelihood that a hepatocellular carcinoma (HCC) will respond to transcatheter arterial chemoembolization (TACE) using a gene signature of 14 or 15 genes. Also provided are nucleic acid probes and kits for detecting the gene signature.

Abstract: The present invention relates to methods of treating cancer patients carrying one or more specific fusion genes. It is based, at least in part, on the discovery that the protein encoded by the MAN2A1-FER fusion gene exhibits kinase activity and the use of tyrosine kinase inhibitors targeting MAN2A1-FER in a cancer other than prostate, for example hepatocellular cancer, led to dramatic improvement of survival of animals xenografted with the cancer.

Abstract: The present invention relates to the field of medicine and molecular diagnostics. In particular, it relates to a novel RNA next generation sequencing-based profiling assay allowing simultaneous detection of transcripts and alternative splice variants thereof and mutations therein, from genes involved in disease.

Abstract: The invention relates to method for detecting HPV-induced high-grade precancerous lesions, HPV-induced invasive cancers and nonHPV-induced gynaecological and anogenital cancers, said method comprising detection of a methylation classifier consisting of the genes LHX8, ASCL1 and ST6GALNAC5 and their regulatory sequence in a cell whereby such hypermethylation indicates the presence of HPV-induced precursor lesions with invasive potential, HPV-induced invasive cancers and nonHPV-induced gynaecological and anogenital cancers. The invention further comprises the use of the methylation classifier consisting of genes LHX8, ASCL1 and ST6GALNAC5 and their regulatory sequence in such a method and a test kit for the detection of LHX8. ASCL1 and/or ST6GALNAC5 methylation.

Abstract: In an aspect, there is provided a method for diagnosing or prognosing a subject with cancer, the method comprising: providing cancer DNA sequencing data from a cancer sample comprising cancer DNA from the subject; comparing the cancer DNA sequencing data with control DNA sequencing data to determine genetic aberrations; determining, from the genetic aberrations, the clonal and subclonal populations present in the sample; constructing a phylogenetic map of the clonal and subclonal populations; assigning to the subject a risk level associated with a better or worse patient outcome or response to therapy; wherein a relatively higher risk level is associated with a higher level of evolution and number of subclonal populations and a relatively lower risk level is associated with a lower level of evolution and number of subclonal populations.

Abstract: The present invention pertains to a method for detecting whether a cancer disease in a subject is susceptible to a treatment with a Spleen Tyrosine Kinase (SYK) inhibitor by determining the amount or level of a biomarker selected from Hoxa9/Meis1, PU.1 and miR146a in a biological sample from the subject. The invention provides novel treatment approaches based on the detection of the differential expression of the above biomarkers using SYK inhibitors. Also provided are diagnostic kits, and combined therapeutic and diagnostic kits for use in the inventive methods.

Abstract: The present invention relates compositions, methods and kits for characterizing the type of and screening for the existence or predisposition for small cell carcinoma of the ovary, hypercalcemic type (SCCOHT). The invention also relates to a method of treating a mammalian subject having SCCOHT or a predisposition for SCCOHT.

Abstract: Described herein are biomarkers for HPV-associated pre-cancers and cancers such as cervical cancer and cervical intraepithelial neoplasia. The RNA binding protein (RBP) and long-noncoding RNA (lnc-RNA) biomarkers can be detected and used to diagnose HPV-associated pre-cancers and cancers. In addition, early diagnosis of HPV-associated pre-cancers and cancers can facilitate therapeutic intervention in patients, particularly in the pre-cancer stage which can delay or prevent progression to cancer.

Abstract: The present invention provides a novel method to determine the likelihood of effectiveness of a treatment in an individual affected with or at risk for developing cancer. The method involves detecting the presence or absence of Met amplification in an individual. The presence of Met amplification indicates that a Met targeting treatment is likely to be effective. Preferably, the Met targeting treatment is PHA-665752 or PF-02341066. In addition, the present methods allow for the detection of cancer in an individual, wherein the presence of Met amplification indicates that cancer is present and further that it will be treatable, namely with a Met targeting treatment.

Abstract: The invention relates to new nucleophosmin protein (NPM) mutants, corresponding gene sequences and relative uses thereof for diagnosis, monitoring of minimal residual disease, prognostic evaluation and therapy of acute myeloid leukaemia (AML).

Abstract: Nucleic acid oligonucleotide sequences are disclosed which include amplification oligomers and probe oligomers which are useful for detecting multiple types of human papillomaviruses (HPV) associated with cervical cancer. Methods for detecting multiple HPV types in biological specimens by amplifying HPV nucleic acid sequences in vitro and detecting the amplified products are disclosed.

Abstract: Aspects of this disclosure relate to liquid-based workflows for strain engineering. In strain engineering, cells undergo a modification process to acquire a desired change or changes in the cells. Some of the cells accept the change or changes and some do not. Provided herein are liquid-based methods for selecting the population of cells that have accepted the change or changes. Also provided herein are liquid-based methods for removing markers from transformed cells and liquid-based methods for isolating clonally pure populations of cells.

Abstract: The present invention refers to a new strain belonging to the species Lactobacillus paracasei able to convert linoleic acid into conjugated linoleic acid. The present invention refers also to nutritional or food preparations and/or pharmaceutical compositions, comprising the strain Lactobacillus paracasei, useful in the treatment and/or prevention of pathologies and/or physiological states related to conjugated linoleic acid deficiency or in the cases wherein the use of a probiotic is beneficial.

Abstract: Stevia compositions are prepared from Stevia rebaudiana Bertoni extracts. The compositions are able to provide a superior taste profile and can be used as sweeteners, sweetness enhancers, flavors, flavor enhancers in foods, beverages, cosmetics and pharmaceuticals.

Abstract: A method of generating a refined a sugar stream that comprises xylose from a biomass hydrolysis solution, including contacting a biomass hydrolysis solution that includes a population of mixed sugars comprising xylose, an acid, and impurities, with a thermally-phase separable solvent such as a glycol solvent to form an extraction mixture; and separating from said extraction mixture a first stream including the thermally-phase separable solvent, acid, and impurities and a second, refined sugar stream that comprises xylose.

Abstract: Provided is a hot stamped body which includes a base metal, and a plated layer formed on a surface of the base metal, wherein the plated layer includes an interface layer, an intermediate layer, and an oxide layer in order from a base metal side, the interface layer contains one or more kinds of Fe—Al alloy, a total area fraction of the Fe—Al alloy being 99% or more, the intermediate layer 22 contains one or more kinds of Fe—Al—Zn alloy, a total area fraction of the Fe—Al—Zn phase being 50% or more, an average composition of the intermediate layer contains, in mass %, Al: 30 to 50% and Zn: 15 to 30%, and an average film thickness of the oxide layer is 3.0 ?m or less, and Mg content in the oxide layer is 0.05 to 0.50 g/m2.

Abstract: Provided is a coolant spray module system for a heat treatment metal product, comprising: a first coolant spray module (1), wherein the first coolant spray module (1) comprises a first module housing (10), a first module cover (20), and a first nozzle (30), wherein the first nozzle (30) is fixed between the first module housing (10) and the first module cover (20) by first nozzle fixing blocks (13) and second nozzle fixing blocks (23), wherein the coolant spray module system sprays the coolant onto the heat treatment metal product to quench and clean the heat treatment metal product.

Abstract: A warm-workable high-strength steel sheet having superior warm workability and residual ductility after warm working, and a method for manufacturing such steel sheets. The warm-workable high-strength steel sheet has a chemical composition including, in mass %, C: 0.05 to 0.20%, Si: not more than 3.0%, Mn: 3.5 to 8.0%, P: not more than 0.100%, S: not more than 0.02%, Al: 0.01 to 3.0% and N: not more than 0.010%, the balance being Fe and inevitable impurities. The steel sheet has a microstructure that includes, in area fractions, 10 to 60% retained austenite, 10 to 80% ferrite, 5 to 50% martensite and 0 to 5% bainite, the C content in the retained austenite being less than 0.40 mass %.

Abstract: A method for producing a grain-oriented electrical steel sheet according to an embodiment of the present invention comprises the steps of: heating a slab satisfying the following formula 1 and including, by wt %, 3.2 to 4.0% of Si, 0.03 to 0.09% of C, 0.015 to 0.040% of A1, 0.04 to 0.15% of Mn, 0.001 to 0.005% of N, 0.01% or less of S (exclusive of 0%), and the balance of Fe and other inevitable impurities; hot rolling the slab to produce a hot rolled sheet; hot band annealing the hot rolled sheet to at a cracking temperature of 900 to 980? for 30 to 300 seconds; cold rolling the hot rolled sheet, which has completely been hot band annealed, to produce a cold rolled sheet; subjecting the cold rolled sheet to primary recrystallization annealing; and conducting secondary recrystallization annealing for the cold rolled sheet which has completely been primary recrystallization annealed. [Mn]×[S]?0.0004??[Formula 1] (wherein [Mn] and [S] are contents (wt %) of Mn and S in the slab, respectively.

Abstract: Provided are a high-strength galvanized steel sheet having excellent delayed fracture resistance by reducing the diffusible hydrogen content in the steel and a method for producing the same. The high-strength galvanized steel sheet includes a steel sheet having a prescribed composition and a microstructure including martensite and tempered martensite, the total area fraction of the martensite and the tempered martensite being 30% or more, and a galvanizing layer formed on the surface of the steel sheet. The diffusible hydrogen content in the high-strength galvanized steel sheet is 0.50 wt. ppm or less. The half-width of the hydrogen release peak of the high-strength galvanized steel sheet is 70° C. or less. The diffusible hydrogen content and the half-width of the hydrogen release peak are determined by a prescribed analysis method.

Abstract: A hot rolled steel sheet having a chemical composition consisting of, in mass %, C: 0.020-0.180%, Si: 0.05-1.70%, Mn: 0.50-2.50%, Al: 0.010-1.000%, N: 0.0060%, P?0.050%, S?0.005%, Ti: 0-0.150%, Nb: 0-0.100%, V: 0-0.300%, Cu: 0-2.00%, Ni: 0-2.00%, Cr: 0-2.00%, Mo: 0-1.00%, B: 0-0.0100%, Mg: 0-0.0100%, Ca: 0-0.0100%, REM: 0-0.1000%, Zr: 0-1.000%, Co: 0-1.000%, Zn: 0-1.000%, W: 0-1.000%, the balance: Fe and impurities, wherein a metal microstructure includes, in area %, at a position ¼ W or ¾ W from an end face of the steel sheet and ¼ t or ¾ t from a surface, martensite: more than 2%-10%, retained austenite <2%, bainite 40%, pearlite 2%, the balance: ferrite, an average circle-equivalent diameter of a metallic phase constituted of martensite/retained austenite is 1.0-5.0 ?m, an average of minimum distances between adjacent metallic phases is 3 ?m or more, and a standard deviation of nano hardness is 2.0 GPa or less.

Abstract: A process for manufacturing a cold-rolled steel sheet with a strength of at least 1200 MPa and an elongation at break greater than 10%. A steel is provided having a microstructure comprising 65 to 90% bainite. A semifinished product is cast from the steel and heated to a temperature greater than 1150° C. The semifinished product is hot rolled to obtain a hot-rolled sheet; the coiled and pickled. Cold-rolling occurs with a reduction ratio of between 30 and 80% so as to obtain a cold-rolled sheet; and then reheating occurs at a rate Vc between 5 and 15° C./s up to a temperature T1 between Ac3 and Ac3+20° C. and held at said temperature T1 for a time t1 between 50 and 150 s. The sheet is cooled at a rate VR1 greater than 40° C./s but below 100° C./s down to a temperature T2 between (Ms?30° C. and Ms+30° C.). The sheet is maintained at temperature T2 for a time t2 between 150 and 350 s, and then cooled at a rate VR2 of less than 30° C./s down to an ambient temperature.

Abstract: A cold rolled steel wire having the following chemical composition expressed in percent by weight, 0.2?C %?0.6, 0.5?Mn %?1.0, 0.1?Si?0.5%, 0.2?Cr?1.0%, P?0.020%, S?0.015%, N?0.010%, and optionally not more than 0.07% Al, not more than 0.2% Ni, not more than 0.1% Mo and not more than 0.1% Cu, the balance being iron and the unavoidable impurities due to processing. This wire has a microstructure including bainite and, optionally, up to 35% acicular ferrite and up to 15% pearlite. A fabrication method and flexible conduits for hydrocarbon extraction are also provided.

Abstract: A method for manufacturing a thermally treated steel sheet is described. The method includes: A. preparation step containing: 1) a selection substep, wherein: a. mtarget and a chemical composition are compared to a list of predefined products, whose microstructure contains predefined phases and predefined proportion of phases, and a product having a microstructure mstandard closest to mtarget and TPstandard is selected, including at least a heating, a soaking and a cooling steps, to obtain mstandard, b. a heating path, a soaking path including a soaking temperature Tsoaking, a power cooling of the cooling system and a cooling temperature Tcooling are selected based on TPstandard and 2) a calculation substep, wherein through variation of the cooling power, new cooling paths CPx are calculated based on the product selected in step A.

Abstract: A method of operating a pelletizing plant for additional treatment of iron making, steelmaking and pellet plant residues, the plant including an indurating furnace in which green pellets are charged and fired to produce hard pellets, where the method includes: preparing iron ore green pellets; and charging and firing said green pellets in an indurating furnace, such that agglomerates of by-products are charged and fired with the green pellets in the indurating furnace, thereby producing hardened pellets and hardened agglomerates of by-products; a pelletizing plant is also presented including a pelletizing section, an indurating furnace, an agglomerating section for producing agglomerates of by-products, where a feeding arrangement is configured to charge agglomerates of by-products into said indurating furnace, together with the green pellets.

Abstract: A method for recovery of platinum group metals from a spent catalyst is described. The method includes crushing the spent catalyst to obtain a catalyst particulate material including particles having a predetermined grain size. The method includes subjecting the catalyst particulate material to a chlorinating treatment in the reaction zone at a predetermined temperature for a predetermined time period by putting the catalyst particulate material in contact with the chlorine containing gas. The method also includes applying an electromagnetic field to the chlorine-containing gas in the reaction zone to provide ionization of chlorine; thereby to cause a chemical reaction between platinum group metals and chlorine ions and provide a volatile platinum group metal-containing chloride product in the reaction zone. Following this, the volatile platinum group metal-containing chloride product is cooled to convert the product into solid phase platinum group metal-containing materials.

Abstract: Embodiments of the invention relate to a process for removing fluoride from a solution or suspension containing zinc, in particular a solution of zinc sulfate, a defluoridated solution of zinc sulfate obtainable by such a process, its use as well as processes for producing zinc and hydrogen fluoride or hydrofluoric acid. The process for removing fluoride comprises (i) providing a solution or suspension A containing zinc, wherein the solution or suspension A containing zinc further contains fluoride ions; (ii) adding a solution B containing a dissolved salt of a rare earth element to the solution or suspension A containing zinc, wherein a solid comprising a rare earth element fluoride and a solution C containing zinc are formed; and (iii) separating the solid from the solution C containing zinc, wherein the solution C containing zinc has a lower concentration of fluoride ions than the solution or suspension A containing zinc.

Abstract: The invention relates to a powder mixture for producing an alloy, a powder metallurgy process for producing a material, a material, and a medical implant made from it.

Abstract: An article comprises a bulk layer of an aluminum-alloy composite and a surface layer. The bulk layer comprises an aggregate dispersed in an aluminum-alloy matrix, the aggregate being solid and unreactive in a melt of the aluminum-alloy matrix, and having an average particle size of 100 microns or less. The surface layer comprises an anodized form of the bulk layer.

Abstract: Provided is a Corson alloy having improved bending workability and also having high dimensional accuracy after press-working. A copper alloy strip which is a rolling material, the rolling material containing from 0 to 5.0% by mass of Ni or from 0 to 2.5% by mass of Co, the total amount of Ni+Co being from 0.2 to 5% by mass; from 0.2 to 1.5% by mass of Si, the balance being copper and unavoidable impurities, wherein the rolling material has a surface satisfying the relationship: 1.0?I(200)/I0(200)?5.0; wherein an area ratio of Cube orientation {100} <001> is from 2 to 10% in EBSD measurement of a rolling parallel cross section; and wherein a ratio: (an average crystal grain size of Cube orientation {100} <001> of the rolling parallel cross section)/(an average crystal grain size of the rolling parallel cross section) is from 0.75 to 1.5.

Abstract: An aluminum alloy material according to an embodiment is based on aluminum and contains 2.5 mass % or more and 4.0 mass % or less of manganese, 0.01 mass % or more and 0.12 mass % or less of zirconium, and 0.55 mass % or more and 0.60 mass % or less of iron.

Abstract: Provided is a super-hard sintered material which contains 5 to 55 mass % of W and a WC balance containing inevitable impurities and is excellent in high hardness and high strength.

Abstract: The present invention refers to a vermicular cast iron alloy specially designed for internal combustion engine blocks and heads having special requirements of mechanical strength and fatigue strength. Vermicular iron alloy with high mechanical strength and high fatigue strength for the production of internal combustion engines blocks and heads characterized by having a microstructure of pearlitic matrix and predominantly vermicular graphite (>70%) and presence of graphite nodules in up to 30%, wherein its graphite microstructure is described by the Microstructure Factor (FM), as defined below, with Microstructure Factor values higher than 0.94.

Abstract: The invention relates to a product made of an aluminium-based alloy comprising, by wt. %, Cu: 2.4-3.2; Li: 1.6-2.3; Mg: 0.3-0.9; Mn: 0.2-0.6; Zr: 0.12-0.18; such that Zr??0.06 *Li+0.242; Zn: <1.0; Ag: <0.15; Fe+Si?0.20; optionally, at least one element selected from Ti, Sc, Cr, Hf and V, the content of the element, if selected, being: Ti: 0.01-0.1; Sc: 0.01-0.15; Cr: 0.01-0.3; Hf: 0.01-0.5; V: 0.01-0.3; other elements ?0.05 each and ?0.15 in total; the remainder being aluminium.

Abstract: Heat treatment of an Alloy 282 which has been subjected to an initial solution annealing followed by cooling can be heat treated by heating the Alloy 282 to a temperature between 954° C. and 1010° C. until the gamma prime (??) phase is sufficiently dissolved, and cooling the Alloy 282 to a temperature a sufficiently low temperature, and at a sufficiently high cooling rate, to suppress gamma prime precipitation. A component such as a turbine exhaust case and a gas turbine engine made of said alloy can be heat treated in the above manner.

Abstract: Disclosed is a method of heat-treating a titanium alloy part resulting from an additive manufacturing procedure, including arranging the titanium alloy part in an oven; heating to a first annealing temperature; maintaining the first annealing temperature for a first annealing duration; heating to a second annealing temperature, wherein the second annealing temperature exceeds the first annealing temperature; and subsequently cooling the titanium alloy part to room temperature. Further disclosed is a titanium alloy part that has been heat-treated using such a method.

Abstract: To provide a hot-dip Al alloy coated steel sheet which is excellent in post-painting corrosion resistance and post-working corrosion resistance. Disclosed is a hot-dip Al alloy coated steel sheet comprising a coating formed by a coating layer and an interfacial alloy layer present at an interface between the coating layer and a base steel sheet, in which the interfacial alloy layer contains Mn, and the coating layer contains Mg2Si having a major axis length of 5 ?m or more.

Abstract: The disclosure discloses a frame component, which includes a frame with a positioning slot in an inner edge thereof, a fabrication strip with grooves, and a plurality of support pieces; where the fabrication strip is detachably arranged in the positioning slot, and the plurality of support pieces are fixed in the grooves to form a support plane for supporting a mask.

Abstract: Embodiments are directed to a method of forming a magnetic stack arrangement of a laminated magnetic inductor having a high frequency peak quality factor (Q). A first magnetic stack is formed having one or more magnetic layers alternating with one or more insulating layers in a first inner region of a laminated magnetic inductor. A second magnetic stack is formed opposite a surface of the first magnetic stack in an outer region of the laminated magnetic inductor. A third magnetic stack is formed opposite a surface of the second magnetic stack in a second inner region of the laminated magnetic inductor. The insulating layers are formed such that a thickness of an insulating layer in the second magnetic stack is greater than a thickness of an insulating layer in the first magnetic stack.