Abstract: A method of preparing a ddx27-deletion zebrafish mutant, including: determining a target of ddx27 knockout on a sixth exon of the ddx27 in a zebrafish and designing a gRNA sequence; using primers T7-ddx27-sfd and tracr rev for PCR amplification with a pUC19-gRNA scaffold plasmid as a template; purifying and transcribing the PCR product obtained in vitro to produce gRNA; introducing the gRNA and a Cas9 protein into the zebrafish; and culturing the zebrafish to obtain a zebrafish ddx27 mutant of stable inheritance. In addition, the application also discloses a phenotype of the ddx27-deletion zebrafish mutant, which plays an important role in investigating the biological function.

Abstract: The present invention relates to the engineering an animal model, preferably mammalian models, more preferably a rat model representing Charcot-Marie-Tooth disease 2A (CMT2A) harboring the p.Arg364Trp or p.His361Tyr Mfn2 mutation, whose human counterpart results in a severe, early-onset axonal neuropathy. A model having the p.Arg364Trp Mfn2 mutation is based on a mutation made using zinc finger endonuclease technology in fertilized rat eggs. Cohorts of mutants and wild type littermates were characterized behaviorally and shown to develop multiple motor deficits that worsened over time. Separate cohorts of mutant and wild type rats sacrificed at 7, 40, and 48 weeks and analyzed by light microscopy showed a reduced density of myelinated axons and active axonal degeneration in distal but not proximal nerves, as well as axonal degeneration in the fasciculus gracilis of the cervical spinal cord at 40 and 48 weeks.

Abstract: Non-human animals, and methods and compositions for making and using the same, are provided, wherein the non-human animals comprise a humanization of a Programmed cell death 1 (Pdcd1) gene. The non-human animals may be described, in some embodiments, as having a genetic modification to an endogenous Pdcd1 gene so that the non-human animals express a PD-1 polypeptide that includes a human portion and an endogenous portion (e.g., a non-human portion).

Abstract: In a housing (1), an internal space capable of housing an insect is formed, an opening (11) through which the internal space and an outside communicate with each other is formed, and a portion other than the opening (11) is closed so that the insect is incapable of passing through the portion. Rails (2) in a pair are disposed in parallel with each other on both sides of the opening (11) across the opening (11) on an outer surface of the housing (1), maintain a gap between a face, on which the opening (11) is formed, of the housing (1) and a cover (3) to have a size preventing the insect from passing through the gap, and slidably retain the cover (3) on the external side of the housing (1). A sample holder retains a sample, is slidably retained by the rails (2), and enables the sample to be exposed from the opening (11) to the internal space when being slidably retained by the rails (2) in the state of retaining the sample.

Abstract: The present invention relates to a degenerative disease model Drosophila. More particularly, a technique is disclosed for alleviating the phenotypes of a degenerative disorder symptom of Drosophila. Using this technique, illumination with low dose radiation on a degenerative disease model Drosophia alleviates symptoms of the degenerative disease.

Abstract: An attachment kit configured to attach to a landing net, the attachment kit including an arm bed including: a receptacle having a concave surface for contacting a user's forearm or elbow, and a bed attachment portion operably connected to the receptacle, the bed attachment portion configured to contact a pole portion of the landing net for affixing the arm bed to the pole portion.

Abstract: A lighting fish attracting device includes a fixed support, a solar panel, a battery, a lamp, and at least one floating member that is configured to float on a water surface when in use. The fixed support and the battery are mounted on the at least one floating member. The solar panel and the lamp are mounted on the fixed support. The battery is connected to the lamp and the solar panel. The lamp is located on a dark side of the solar panel, and the dark side is configured to face the water surface when in use.

Abstract: A device for converting a container into an insect trap. A device opening is configured to allow an insect to enter an interior cavity of the container and configured to prevent the insect from exiting the interior cavity of the container using a unidirectional barrier.

Abstract: In accordance with various embodiments of the disclosed subject matter, an apparatus for killing insects generally comprising a backplate, having pivotally mounted thereto a first flange comprising an electrically conductive material and a second flange, the first and second flange being of similar shape; a flange operating mechanism, operably coupled to the first and second flanges, and configured for causing a position of the first and second flanges to move from an open position to a closed position; and a power switch, for selectively providing electrical energy to the electrically conductive material of the first flange such that an insect in contact therewith may be killed.

Abstract: Embodiments of the inventive concept provide a portable organic entity termination applicator unit. The applicator unit can include an organic herbicide reservoir or can otherwise be an herbicideless applicator unit. The applicator unit can include an outer housing, a cold water supply line, an organic herbicide reservoir to hold organic herbicide, an herbicide adjustment valve for controlling a liquid mixture, a heater core to pre-heat the herbicide water liquid mixture, and a delivery pipe to expel the heated liquid. The applicator unit can include a fuel canister receptacle to receive a fuel canister. The heater core may heat the liquid using fuel stored in the fuel canister.

Abstract: A blower spray device is provided having a housing which comprises an electrically driven blower and a spray nozzle, and having a liquid supply line opening into the spray nozzle. The housing has a funnel-shaped intake channel and a tubular blow-out channel, and an electrical valve is provided in the liquid supply line to activate and deactivate the liquid supply to the spray nozzle. An electronic controller having a regulating unit for the electric blower is further provided to adjust the air flow velocity.

Abstract: A firearm steady rest includes a tree stand unit and a universal steady rest unit. The tree stand unit has a tubular stand mount and U-bolts to secure the tree stand unit to a tree stand. A bottom slide is slidably received within the tubular stand mount while a tubular rest mount is pivotally connected to the bottom slide. A stand clamp secures the bottom slide within the tubular stand mount while a rest clamp secures the universal steady rest unit within the tubular rest mount. The universal steady rest unit includes a steady mount having a circular post region proportioned to rotate within the tubular rest mount. A steady rest is pivotally coupled to the steady mount via a pivot plate. A tubular extension member may also be included. The firearm steady rest may fold into a bundle measuring less than 4 inches by 4 inches by 28 inches.

Abstract: The present invention provides a method and apparatus for tissue, such as an allograft, storage and preservation for extended periods of time at room temperature in a sterile tissue culture chamber. The invention further provides a process for maintaining the sterility of tissue using the apparatus as described.

Abstract: A cell freeze-drying system, comprising: a temperature-elevated nitrogen supplier, a freeze-drying operation box, and a gas discharger, which are connected in that order using a first connection pipe. The temperature-elevated nitrogen supplier supplies warmed nitrogen to the freeze-drying operation box. A cell sample frozen by liquid nitrogen is placed in the freeze-drying operation box, and then the freeze-drying operation box, which is preset to a temperature suitable for placing the cell sample frozen by liquid nitrogen, is warmed up in a preset mode while continuously being supplied with the warmed nitrogen to sublimate solid state water in the cell sample into gaseous water. The gas discharger is used to discharge the gaseous nitrogen and the gaseous water. Further disclosed is a cell freeze-drying method. The invention can effectively address an issue of cell damage and contamination during freeze-drying, and realizes safety, efficiency, and low costs for cell freeze-drying.

Abstract: A method for preparing a transplant obtained from animal or human tissue is provided. The transplant is first deposited in a first liquid that comprises at least one substance that initiates and/or activates collagen cross-linking. After removing the transplant from the first liquid, the transplant, now moistened with said first liquid, is exposed to ultraviolet radiation and then to accelerated low-energy electrons.

Abstract: Described herein are biodegradable composites and methods of forming the same. The composites generally comprise a base polymer, a plant-based filler material, and optionally a fragrant additive. The fragrant additives may be used for attracting animals to or repelling animals from an area. Methods of forming the biodegradable composites include an extrusion process using a melt formed from the base polymer and the plant-based filler material.

Abstract: An aqueous slurry composition containing microcapsules dispersed in an aqueous medium. The microcapsules contain an ionic acrylate copolymer shell encapsulating an oily medium. Upon drying, the microcapsules release the oily medium. The slurry is useful in delivering a variety of water-insoluble substances via an aqueous medium including cosmetic or agricultural agents.

Abstract: A disinfecting composition for killing pathogens on various surfaces is provided. The disinfecting composition is environmentally friendly and is certified natural according to the ECOCERT certification for detergents. The disinfecting composition generally includes one or more Ci-8 alcohols and at least three surfactants. The disinfecting composition passes one or more of the following European standards at a contact time of less than or equal to about 1 minute: EN 13697, EN 13727, EN 1275, EN 1650, and EN 13624.

Abstract: The present invention relates to a herbicidal composition comprising pethoxamid. The invention also relates to methods and uses for controlling undesirable vegetation, in particular in crops.

Abstract: The present invention relates to a herbicidal composition comprising at least one chloroacetamide selected from acetochlor and pretilachlor. The invention also relates to methods and uses for controlling undesirable vegetation, in particular in crops.

Abstract: The present invention relates to a herbicidal composition comprising imazamox, any of its individual isomers or agriculturally acceptable salts, esters or amides thereof. The invention also relates to methods and uses for controlling undesirable vegetation, in particular in crops.

Abstract: Quinazolinedione-6-carbonyl derivatives of the general formula (I) are described as herbicides. In this formula (I), R1, R2, X and W are radicals such as hydrogen, alkyl and halogen. Z is a chalcogen. Q is a five-membered heterocycle.

Abstract: The present invention relates to compounds of Formula (I), or an agronomically acceptable salt of said compounds wherein R1a, R1b, R2, X, A, Ra, Rb, Rc, Rd and m are as defined herein. The invention further relates to herbicidal compositions which comprise a compound of Formula (I), to their use for controlling weeds. The invention further relates to intermediate compounds used to produce compounds of Formula (I).

Abstract: Provided herein are compositions and methods for inhibiting the sublimation of solid compounds, such as 3,5-disubstituted-1,2,4-oxadiazoles, from the surface of a substrate (e.g., seeds).

Abstract: The present invention relates to a herbicidal composition comprising specific inhibitors of protoporphyrinogen oxidase (PPO inhibitors) selected from flumioxazin and sulfentrazone. The invention also relates to methods and uses for controlling undesirable vegetation, in particular in crops.

Abstract: The present invention relates to a herbicidal composition comprising saflufenacil. The invention also relates to methods and uses for controlling undesirable vegetation, in particular in crops.

Abstract: The present invention relates to a herbicidal composition comprising specific pigment synthesis inhibitors selected from beflubutamide, flurtamone and clomazone. The invention also relates to methods and uses for controlling undesirable vegetation, in particular in crops.

Abstract: The invention relates to novel compounds of the formula (I) in which X, R1, R2, R3 and n have the definitions given above, to the use thereof as acaricides and/or insecticides for control of animal pests and to processes and intermediates for preparation thereof.

Abstract: The disclosure provides compounds useful as insect repellents, compositions comprising such repellents, and methods of repelling an arthropod using such compounds and compositions. The disclosure further provides insect traps and method for identifying ligands and cognates for biological molecules.

Abstract: A process is described for stabilizing an aqueous preparation against microbial growth. Also described, is the aqueous preparation stabilized against microbial growth as well as the use of a source of at least one metal oxide and/or its hydrated form for reducing the amount of an antimicrobial agent against at least one strain of bacteria and/or at least one strain of yeast and/or at least one strain of mold in an aqueous preparation. The use of a composition including at least one antimicrobial agent and a source of at least one metal oxide and/or its hydrated form as an antimicrobial composition in an aqueous preparation, is also described.

Abstract: Silver ion solutions for use in agricultural spray formulations prepared for use in agricultural environs as an effective anti-bacterial spray, without environmentally hazardous or undesirable phytotoxicity. An iodine solution, a lime juice or a cranberry juice adjunct may be added to the silver ion solution in deionized water, to increase efficacy, especially in the ultra-low concentration range of the solution from parts per billion to parts per trillion of silver ion. The organically certifiable silver ion agricultural solution is applied externally to an agricultural commodity in the orchard or field, such as apples, cherries, pears, or any other fruit or vegetable.

Abstract: The present invention relates to the process of constructing plant-site specific functional microbiomes to screen, identify plant and soil microbes with beneficial traits, and to apply the functional microbiome, microbe or microbial consortia for improving the growth and health of plants cultivated on normal, stressed or marginal lands and for improving soil health, the removal and/or stabilisation of organic and inorganic pollutants, and the enhancement of soil microbial ecosystem functions.

Abstract: Compositions and methods for treating or preventing a fungal plant disease are provided. Such compositions and methods comprise a combination of one or more chemical fungicides and one or more fungicidal bacterial or fungal strains, or an active variant thereof, that controls one or more fungal pathogens that cause fungal plant disease. Methods for growing a plant susceptible to a fungal plant disease and methods for controlling a fungal plant pathogen are also provided.

Abstract: The present invention relates to a composition comprising a biologically pure culture of a fungicidal Paenibacillus sp. strain comprising a variant fusaricidin synthetase lacking a functional adenylation domain in the third module. The present invention also provides a composition comprising a biologically pure culture of a fungicidal Paenibacillus sp. strain or a cell-free extract thereof comprising at least one Paeniserine and at least one Paeniprolixin. Also provided are isolated compounds and methods of treating a plant to control a plant disease with the disclosed compositions and compounds.

Abstract: A cooking appliance (100) is disclosed comprising a cooking chamber (170), a heating stage (150) for heating a food product within the cooking chamber and a humidity adjustment stage (160) for adjusting the humidity within the cooking chamber. The cooking appliance further comprises a user interface (110) configured to allow a user to specify a texture of said food product and a degree of browning of said food product; and a controller (120) responsive to the user interface and configured to control the heating stage and the humidity adjustment stage in accordance with an algorithm having the user-specified texture of said food product and the user-specified degree of browning of said food product as parameters.

Abstract: The invention relates to a device for portioning a dough mass, comprising a receiving chamber for holding said dough mass comprising a discharge opening, a pair of opposing rotary cutters provided at the discharge opening and arranged for rotation in mutually opposite directions, wherein each cutter comprises at least one blade rotatable around a rotary axis, which blade cooperates with the blade of the other cutter for cutting the dough mass into separate portions, and a release agent delivery system for applying a release agent onto the blades of the rotary cutters, wherein the release agent delivery system is configured for applying the release agent on opposing sides of each of the blades. The invention further relates to a release agent delivery system for use in such a device and a method for portioning a dough mass using such a device.

Abstract: The invention relates to a device for guiding and supplying dough to a dough processing or transporting means comprising a receiving chamber with at least one belt conveyor comprising a conveyor belt forming at least part of a side wall of the receiving chamber. The receiving chamber is further provided with at least two sealing strips mounted at opposing side ends of the conveyor, wherein the sealing strips create a seal between the conveyor belt and an adjacent stationary wall forming another part of the side wall of the receiving chamber. The invention further relates to a chunker for subdividing dough into smaller parts comprising said device.

Abstract: A dough piece mold is configured to receive at least one dough piece during a fermentation and baking process. The dough piece mold has at least one machine readable identification label with a ceramic-based carrier, containing identification data, which include mold type data and unambiguous individualization data for the respective dough piece mold. A dough processing installation can be used to perform a dough processing method using a plurality of such dough piece molds.

Abstract: The invention pertains to a soft-baked biscuit that is high in protein, and methods of making the same, that includes a protein component, a multi-textured mouth feel, and crispy inclusions contrasting with a moist and chewy matrix. The protein component may include a powdered protein source and a particulate protein source. The particulate protein source may be in the form of protein crisps, pops, tots, flakes, puffs, inclusions, pellets, bits, and/or particles. In some embodiments of the invention, the protein source includes a powdered protein source in an amount of about 35% by weight of the protein source to about 85% by weight protein powder and about 15% by weight to about 65% by weight particulate protein such as protein crisps. In another embodiment, the softbaked biscuit may include a protein component and a grain flour, wherein the flour is at least 4% by weight of the soft-baked biscuit.

Abstract: The disclosure relates to a series of independent human-induced non-transgenic mutations found at one or more of the Lip1 genes of a plant; plants having these mutations in one or more of their Lip1 genes; and a method of creating and finding similar and/or additional mutations of Lip1 by screening pooled and/or individual plants. The plants disclosed herein exhibit decreased lipase activity without having the inclusion of foreign nucleic acids in their genomes. Additionally, products produced from the plants disclosed herein exhibit increased hydrolytic and oxidative stability and increased shelf life without having the inclusion of foreign nucleic acids in their genomes.

Abstract: An intestines processing system and method including a conveyor track with carriers, a tissue breaking section and a gallbladder removal station. The carrier can be equipped to move the intestines package through the tissue breaking section for breaking at least some of the tissue connections in the intestines package below the gallbladder, and to move the intestines package through the gallbladder removal station for removing the gallbladder from the remainder of the intestines package.

Abstract: The invention relates to an evisceration tool, designed and configured for removing internal organs and in particular an intestine pack from the body of slaughtered poultry, comprising a spoon-shaped basic body, wherein the basic body has a spoon base (12) and a spoon edge which extends around at least part of the spoon base to form a spoon bowl, which is distinguished in that the basic body is made at least partly of a resilient material, wherein the resilient material is on the one hand flexible enough to adapt to a contour of the body during removal of the internal organs and on the other hand stiff enough to remove the internal organs from the body.

Abstract: An indoor smoker includes a firebox defining a smoldering chamber and a smoke tray positioned within the smoldering chamber and having a plurality of chambers, each having a dedicated smoldering heater. The plurality of chambers may be thermally isolated to prevent cross-ignition of combustible material between chambers. In addition, the smoldering heaters may be selectively energized to selectively smolder the combustible material in each of the plurality of chambers.

Abstract: An indoor smoker includes a smoking chamber, a fresh air supply duct, a firebox defining a smoldering chamber, and an exhaust duct. An air handler is operably coupled to the exhaust duct and is configured for urging a flow of fresh air and/or smoke through the smoking chamber and out the exhaust duct which contains a catalytic converter. A flow regulating device, such as a rotary valve, is rotated between angular positions to control the flows of air and smoke, e.g., to facilitate a smoking process, a cold smoking process, a pause in the smoking process, or process termination.

Abstract: An apparatus for blanching segmented avocados uses a conveyor system for transporting the avocados from one end of a blanching chamber to an opposite end, the conveyor system having a perforated conveyor belt positioned on two or more drive rollers for transporting the avocados into and out of the blanching chamber and driven by a drive mechanism. The blanching chamber includes a shroud overlying the conveyor belt and in longitudinal alignment in relation thereto to at least partially enclose steam produced from a bath underlying the conveyor belt and in longitudinal alignment with the shroud and the conveyor belt. During use, the steam is at least partially enclosed by the shroud thereby exposing the segmented avocados positioned on the perforated conveyor belt to steam and blanching the avocados in the blanching chamber at a blanching pressure, at least a portion of which is not sealed from atmosphere.

Abstract: The present invention relates to purified and concentrated human milk oligosaccharide compositions and methods of making and using the same.

Abstract: The present application relates to tofu comprising soy, allulose and a coagulant. The present application also relates to a method for manufacturing tofu using allulose.

Abstract: The invention relates to an oil-continuous composition comprising at least 30 wt. % of a structured continuous oil phase and less than 10 wt. % water, said structured continuous oil phase comprising: 96-99.7 wt. % fat, said fat having a solid fat content at 20° C. (N20) of 0-50% and a liquid oil content at 20° C. that equals 100%-N20; particulate anhydrous non-defibrillated cell wall material from carrot parenchymal tissue, said particulate anhydrous non-defibrillated cell wall material having a particle size of between 25 ?m and 500 ?m; wherein the particulate anhydrous non-defibrillated cell wall material is present in the structured continuous oil phase in a concentration of 0.3-8% by weight of the liquid oil. The aforementioned particulate cell wall material is capable of structuring liquid oil at very low concentrations.

Abstract: A tea extraction method may comprise performing a multistage pressure extraction process. Each extraction stage may comprise forcing a volume of water through a bed of tea material held in a tea material receiver. Each extraction stage may be performed at an extraction stage pressure and a water temperature. Each extraction stage may be separated by a soak stage.

Abstract: The present invention relates to a coffee drink composition comprising allulose, oligosaccharide and coffee extract, and a method of preparation thereof, and it provides a coffee drink composition having a high sugar content and a low calorie and can give more excellent sensory properties to coffee drinks such as Americano.