Abstract: Methods, primers and probes are provided for the isothermal amplification and detection, without denaturation, of double stranded nucleic acid targets for polymerase strand displacement amplification (“iSDA”). The methods and compositions disclosed are highly specific for nucleic acid targets with high sensitivity, specificity and speed that allow detection of clinical relevant target levels. The methods and compositions can easily be used to amplify or detect nucleic acid targets in biological samples.

Abstract: The present invention relates to a method, in particular an in vitro method, for identifying certain immune cells of a mammal, comprising analysing the methylation status of at least one CpG position in the gene CCR6 and/or BLR1 or an orthologous or paralogous gene thereof, and the use of DNA-methylation analysis of the genes of the proteins CCR6 and/or BLR1 for a detection and quality assurance and control of certain immune cells. In particular, the present invention relates to analysing the methylation status of at least one CpG position in the gene CCR6 in T cells. Furthermore, the present invention relates to a kit for performing the above methods, as well as to respective uses.

Abstract: The present disclosure relates to methods for determining the amount or concentration of a nucleic acid of interest in an unprocessed sample by analyzing a processed sample and a reference sample with digital polymerase chain reaction (dPCR).

Abstract: Embodiments of the present invention are directed toward devices, systems, and methods for purifying nucleic acids to conduct polymerase chain reaction (PCR) assays. In one example, a method includes generating complexes of silica beads and nucleic acids in a lysis buffer, transporting the complexes through an immiscible fluid to remove interfering compounds from the complexes, further transporting the complexes into a density medium containing components required for PCR where the nucleic acids disassociate from the silica beads, and thermocycling the contents of the density medium to achieve PCR. Signal may be detected from labeling agents in the components required for PCR.

Abstract: A nucleotide sequence having SEQ ID NO:1, a universal reverse primer having SEQ ID NO:2, and a universal RT primer are provided. In the miRNA detection method, the universal RT primer is used for the cDNA synthesis of a miRNA sample and the universal reverse primer is used for cDNA molecule amplification in qPCR quantitative detection. The universal reverse primer sequence, the nucleotide sequence, and the design rules are used in the method for designing primer, so as to design a primer for qPCR quantitative detection.

Abstract: A method for sequencing a nucleic acid is provided. In certain embodiments the method comprises obtaining a duplex comprising a nucleic acid and a primer, wherein the primer has a nuclease resistant 3? end, combining the duplex with a chain terminator nucleotide and a proof-reading polymerase to produce a reaction in which the polymerase idles on the added chain terminator nucleotide, identifying the chain terminator nucleotide added to the end of the primer; and adding a nuclease-resistant nucleotide to the end of the primer after the polymerase has idled on and removed the added chain terminator nucleotide, thereby producing a duplex comprising the template and an extended primer that has a nuclease resistant 3? end.

Abstract: The present of disclosure provides variant Pol6 polymerase polypeptide, compositions comprising the Pol6 variant polypeptides, and methods for using the variant Pol6 polypeptides for determining the sequencing of nucleic acids, for example, by nanopore sequencing. The variant Pol6 polymerases possess decreased rates of dissociation of template from the polymerase-template complex, which result in increased processivity relative to the parental Pol6 polypeptides from which they are derived.

Abstract: The invention relates to a process for parallel high throughput sequencing of nucleic acid molecules, in particular in the single molecule format.

Abstract: Methods for non-invasive prenatal paternity testing are disclosed herein. The method uses genetic measurements made on plasma taken from a pregnant mother, along with genetic measurements of the alleged father, and genetic measurements of the mother, to determine whether or not the alleged father is the biological father of the fetus. This is accomplished by way of an informatics based method that can compare the genetic fingerprint of the fetal DNA found in maternal plasma to the genetic fingerprint of the alleged father.

Abstract: Provided herein are methods and composition for immune repertoire sequencing and single cell barcoding. The methods and compositions can be used to pair any two sequences originating from a single cell, such as heavy and light chain antibody sequences, alpha and beta chain T-cell receptor sequences, or gamma and delta chain T-cell receptor sequences, for antibody and T-cell receptor discovery, disease and immune diagnostics, and low error sequencing.

Abstract: Methods, compositions, and systems are provided for characterization of modified nucleic acids. In certain preferred embodiments, single molecule sequencing methods are provided for identification of modified nucleotides within nucleic acid sequences. Modifications detectable by the methods provided herein include chemically modified bases, enzymatically modified bases, abasic sites, non-natural bases, secondary structures, and agents bound to a template nucleic acid.

Abstract: The invention relates to improved methods, devices, and kits for identifying and implementing an appropriate treatment regimen for subjects suffering from hypertension.

Abstract: It is intended to provide a kit or device for the detection of liver cancer and a method for detecting liver cancer. The present invention relates to a kit or device for the detection of liver cancer, comprising a nucleic acid capable of specifically binding to miRNA in a sample of a subject, and a method for detecting liver cancer, comprising measuring the miRNA in vitro.

Abstract: Provided herein are kits, compositions and methods for cancer diagnosis, research and therapy, including but not limited to, cancer markers. In particular, the present invention relates to recurrent gene fusions (e.g., recurrent translocations involving TYK2) as diagnostic markers and clinical targets for cutaneous CD30-positive lymphoproliferative disorders (e.g., lymphomatoid papulosis; primary cutaneous anaplastic large cell lymphoma).

Abstract: This document relates to methods and materials for detecting premalignant and malignant neoplasms. For example, methods and materials for determining whether or not a stool sample from a mammal contains nucleic acid markers or polypeptide markers of a neoplasm are provided.

Abstract: Markers associated with Sclerotinia whole plant field resistance are provided. Methods of identifying Sclerotinia resistant and susceptible plants, using the markers are provided. Methods for identifying and isolating QTLs are a feature of the invention, as are QTLs associated with Sclerotinia whole plant field resistance.

Abstract: This invention relates to methods for identifying maize plants that having increased MRCV resistance. The methods use molecular markers to identify and to select plants with increased MRCV resistance. Maize plants generated by the methods of the invention are also a feature of the invention.

Abstract: A method for distinguishing living cells in a living state with high accuracy. A method for distinguishing a desired cell type from a cell group comprising two or more types of cells, using the expression of miRNA as an indicator, wherein the method comprises the following steps: (1) a step of introducing mRNA comprising a marker gene operably linked to the target sequence of miRNA used as an indicator into a cell group; and (2) a step of distinguishing a cell type, using the translation level of the marker gene as an indicator.

Abstract: The invention provides methods and devices for generating optical pulses in one or more waveguides using a spatially scanning light source. A detection system, methods of use thereof and kits for detecting a biologically active analyte molecule are also provided. The system includes a scanning light source, a substrate comprising a plurality of waveguides and a plurality of optical sensing sites in optical communication with one or more waveguide of the substrate, a detector that is coupled to and in optical communication with the substrate, and means for spatially translating a light beam emitted from said scanning light source such that the light beam is coupled to and in optical communication with the waveguides of the substrate at some point along its scanning path. The use of a scanning light source allows the coupling of light into the waveguides of the substrate in a simple and cost-effective manner.

Abstract: A method and system for increasing process performance in a biological process comprising at least one process step, the method comprising: (a) an identification phase, in which at least seven factors are identified, wherein a factor is defined as a feature of or within a process that when modified will affect the performance of the process, and wherein the factors are selected from at least one process factor and at least one genetic factor; (b) a factor screening phase, in which individual and combined contributions to process performance of each of the at least seven factors are determined, such that at least one multi-factorial interaction is identified; and (c) a refinement phase, in which higher order interactions that result in an increase in process performance between the at least seven factors are identified and tested. The method may be incorporated within automated laboratory and design of experiment systems.

Abstract: Methods, devices, and systems for performing polymerase chain reaction (PCR) amplification and melt data acquisition according to a single slug approach in which a single slug in a microfluidic channel fills an entire thermal zone of the microfluidic channel, and the thermal zone used for both PCR temperature cycling and melt data acquisition. A detector may be configured to detect fluorescence from the thermal zone during the PCR temperature cycling for real-time PCR and/or during temperature ramping in the melt data acquisition. Slug position control may be achieved by detecting leading or trailing edges in a slug build target zone into which a slug passes after passing through the thermal zone. The single slug approach may break coupling between one or more events of the PCR amplification and melt data acquisition and enable events to be independently optimized.

Abstract: Provided is a composition containing Lactobacillus reuteri ELF corresponding to a novel lactic acid bacteria strain and 5-aminolevulinic acid. The composition may be significantly effective to prevent and treat degenerative brain disease and have excellent anti-obesity and anti-diabetes effects, such that the present invention may provide various pharmaceutical composition, health foods, and the like, using the same.

Abstract: A novel fungus belongs to the genus Trichoderma whose cellulase can be used to hydrolyze cellulose-based biomass without degradation of xylan contained in the biomass into xylose, and a method produces glucose and xylo-oligosaccharides from a cellulose containing biomass using it. The fungus belonging to the genus Trichoderma includes N- and C-terminal domains of the ?-xylosidase 1 (BXL1) gene, but lacks the Fn3-like domain of the gene due to its disruption. The use of this fungus belonging to the genus Trichoderma results in deletion of ?-xylosidase activity and increase in ?-glucosidase activity. Thus, in hydrolysis of cellulose contained in biomass, xylan contained in the biomass is not degraded into xylose, which enables efficient production of glucose and xylo-oligosaccharides.

Abstract: Described herein are oxime compounds capable of inactivating a nerve agent, blood brain barrier (BBB)-penetration, and/or reactivation of nerve agent-inhibited acetylcholinesterase (AChE) and related methods, systems and compositions for inactivation of one or more nerve agents, therapeutic and/or prophylactic treatment of an individual, and/or decomposition of nerve agent for decontamination.

Abstract: A method of treating an animal skin substrate comprising: agitating the animal skin substrate with a solid particulate material and a treatment liquor; separating the animal skin substrate from effluent comprising the solid particulate material, the treatment liquor and solid waste fragments derived from said substrate; transferring the effluent to a first separator, wherein the first separator is configured to allow the treatment liquor and at least a portion of the solid waste fragments to pass through the first separator but to prevent the solid particulate material from passing through the first separator, and providing a first filtrate stream comprising treatment liquor and solid waste fragments and a first residue comprising solid particulate material and residual solid waste fragments treating said first residue to remove said residual solid waste fragments to provide cleaned solid particulate material, wherein said treating of said first residue comprises retaining said solid particulate material on a

Abstract: The invention relates to an apparatus and to a method for the press hardening of components (2), having at least one furnace (3), having a press (8), which is arranged downstream of the at least one furnace (3), and having a transporting apparatus (4). In order to provide for cycle times which are as short as possible, means (5) for transporting the components (2) are mounted in a moveable manner in the transporting apparatus (4), wherein the means (5) with the components (2) can be moved, along the transporting apparatus (4), through the furnace (3) and into the press (8), wherein the transporting apparatus (4) is continuous between the furnace (3) and the press (8), and wherein the components (2) can be transported from the furnace (3) to the press (8) without being manipulated.

Abstract: A method of treatment of a running ferrous alloy sheet containing at least one easily oxidized element is provided. The method includes a step of immersion of the sheet in a molten oxides bath. The molten oxides bath has a viscosity between 0.3.10?3 Pa·s and 3.10?1 Pa·s, the surface of the bath is contact with a non-oxidizing atmosphere, and the molten oxides are inert towards iron. The residence time of the running sheet in the bath is at least 1 s and the residues of oxides remaining on the surfaces of the sheet at the exit of the bath are eliminated. A treatment line of a ferrous alloy sheet for implementing the method, is provided. The treatment line includes a molten oxides bath having a viscosity between 0.3.10?3 and 3.10?1 Pa·s. The surface of the bath is contact with a non-oxidizing atmosphere, and the molten oxides are inert towards iron. Mechanical devices for eliminating the residues of molten oxides remaining on the surfaces of the ferrous alloy sheet are at the exit of the molten oxides bath.

Abstract: A method for induction surface hardening includes, when in a feed mode, moving an induction coil of a hardening device over a ring surface of a work piece in a treatment direction, to heat the ring surface from an initial zone to a final zone. A sprayer, located downstream of the induction coil in the direction of treatment, is activated to apply quenching liquid to the heated ring surface to both cool and harden the ring surface. An unhardened soft zone is provided between the initial zone and the final zone. A supplementary induction coil, the induction coil and the sprinkler are respectively activated and deactivated separately at the start and end of the hardening, while the relative feed speed between the hardening device and the work piece, and/or the power supplied to each of the supplementary induction coil and the induction coil, are varied at the same time.

Abstract: Provided are a high-strength galvanized steel sheet which can preferably be used as a material for automobile parts and a method for manufacturing the steel sheet. The steel sheet has a C content of 0.15% or less, in which an area ratio of ferrite is 10% or less, an area ratio of bainitic ferrite is 2% or more and 30% or less, an area ratio of martensite is 60% or more and 98% or less, an area ratio of retained austenite is less than 2%, an average grain diameter of martensite adjacent to bainite is 15 ?m or less, a proportion of massive martensite adjacent only to bainite to the whole metallographic structure is 10% or less, and a value (?Hv) calculated by subtracting the Vickers hardness at a position located at 20 ?m from the surface of the steel sheet from the Vickers hardness at a position located at 100 ?m from the surface of the steel sheet is 30 or more.

Abstract: A high-strength cold-rolled steel sheet having a specified chemical composition and a microstructure including ferrite having an average crystal grain diameter of 2 ?m or less in an amount of 10% to 25% in terms of volume fraction, retained austenite in an amount of 5% to 20% in terms of volume fraction, martensite having an average crystal grain diameter of 2 ?m or less in an amount of 5% to 15% in terms of volume fraction, and the balance being a multi-phase structure including bainite and tempered martensite having an average crystal grain diameter of 5 ?m or less, in which a relational expression, 0.35?V2/V1?0.75 (1), is satisfied, where V1 is a volume fraction of phases which are different from ferrite and V2 is a volume fraction of tempered martensite.

Abstract: A high-strength steel sheet having a composition containing C: 0.09% to 0.17%, Si: 0.6% to 1.7%, Mn: 3.5% or less, P: 0.03% or less, S: 0.005% or less, Al: 0.08% or less, N: 0.006% or less, Ti: 0.05% or less, and B: 0.0002% to 0.0030% on a mass basis, the remainder being Fe and inevitable impurities. The steel sheet also has a microstructure containing less than 20% (including 0%) of a ferrite phase, 75% or more (including 100%) of a tempered martensite phase, 10% or less (including 0%) of an untempered martensite phase, and less than 5% (including 0%) of a retained austenite phase in terms of area fraction. The tempered martensite phase has a Vickers hardness of 280 to 340 and a tensile strength of 950 MPa to 1,120 MPa.

Abstract: A hot-rolled steel sheet for a tailored rolled blank is provided that has high tensile strength and is excellent in cold formability. The hot-rolled steel sheet has: a chemical composition that contains, in mass %, C, Si, Mn, P, S, Al, N and Ti, with the balance being Fe and impurities, and that satisfies Formula (1); and a microstructure containing, in terms of area ratio, 20% or more of bainite, wherein 50% or more in terms of area ratio of the balance is ferrite. In the interior of the hot-rolled steel sheet an average value of pole densities of an orientation group {100}<011> to {223}<110> is 4 or less, and a pole density of a {332}<113> crystal orientation is 4.8 or less. In an outer layer of the hot-rolled steel sheet, a pole density of a {110}<001> crystal orientation is 2.5 or more. Furthermore, among Ti carbo-nitrides in the hot-rolled steel sheet, the number density of fine Ti carbo-nitrides having a particle diameter of 10 nm or less is 1.

Abstract: A pre-coated steel strip is provided. The steel strip includes a strip of base steel having a length, a width, a first side, and a second side. The length of the strip is at least 100 m and the width is at least 600 mm. An aluminum or an aluminum alloy pre-coating is on at least part of at least one of the first or second sides of the strip of base steel. A thickness tp of the pre-coating is from 20 to 33 micrometers at every location on at least one of the first or second sides. Processes, coated stamped products and land motor vehicles are also provided.

Abstract: A method for manufacturing a shaft body by welding a plurality of shaft members together and forming the shaft body, the method including: a primary tempering step of subjecting a range in at least one of the shaft members, which is in the vicinity of an end of another shaft member side adjacent thereto, to tempering before the shaft members are welded together so that a strength of an end side of a region thereof is lower than a strength at a side which is opposite to the end of the region thereof; a welding step of welding the shaft members together after the primary tempering step; and a secondary tempering step of tempering the vicinity of a weld part between the shaft members after the welding step.

Abstract: A method for continuously annealing a steel strip includes annealing the steel strip in a vertical annealing furnace, an atmosphere in the furnace being separated by a partition disposed within a space spanning from the heating zone to the soaking zone. An atmosphere gas is supplied from an outside of the furnace into the furnace to form a furnace gas that is discharged from a steel strip entrance at a lower portion of the heating zone, a part of the furnace gas is sucked and discharged into a refiner including a deoxygenator and a dehumidifier such that oxygen and moisture in the part of the furnace gas are removed to form a gas having a lowered dew point, and the gas having a lowered dew point is returned into the furnace. The method further includes controlling a temperature of the steel strip passing through the partition to a range of 550° C. to 700° C.

Abstract: A method for recovering at least one precious metal from an aqueous solution containing the metal and particularly to recovery of silver and optionally one or more other precious metals from overflow of a sedimentation unit such as a thickener, a clarifier or a pond includes subjecting the aqueous solution to a micro and/or nanobubble flotation, wherein the pH of the aqueous solution is at most 1.5.

Abstract: A process for digestion of fine iridium includes (a) alkaline oxidative digestion of 1 part by weight fine iridium and 3 to 20 parts by weight of a combination comprising 40 to 70 parts by weight sodium hydroxide, 15 to 30 parts by weight sodium nitrate, and 10 to 40 parts by weight sodium peroxide in the melt, whereby the sum of the weight fractions equals 100 parts by weight; (b) cooling the digestion material formed in step (a) to 20 to 70° C.; (c) dissolving the acid-soluble fractions of the cooled digestion material in water/halogen hydracid until an acidic aqueous solution with a pH value of ?1 to +1 is obtained; and (d) boiling the acidic aqueous solution obtained in step (c) until the formation of nitrous gases is completed. Insoluble ingredients can be separated from the acidic aqueous solution before or after step (d), if needed.

Abstract: A method for leaching copper from an oxidized copper ore, wherein at least 5% of the copper present in the oxidized copper ore is an oxidized copper compound, the method comprising the method steps of: applying an aqueous solution of a curing agent to the oxidized copper ore to produce a cured ore; forming a leach solution by applying an ammoniacal solution that has an ammonium carbonate content of less than 5 g/L to the cured ore to produce a pregnant leach solution containing copper; and passing the pregnant leach solution to a means for recovering copper.

Abstract: The proposed invention relates to processes for extracting and concentrating radionuclides and can be used in radiochemical technologies when treating liquid radioactive waste. The method for isolating americium from liquid radioactive waste and for separating americium from rare earth elements in a single extraction cycle involves joint extraction of americium and rare earth elements from a nitric acid radioactive solution with a neutral solution of an organic extraction agent in a polar fluorinated solvent; washing an obtained organic phase saturated with metals; and selective back extraction of americium, wherein the extraction agent for joint extraction is N,N,N?,N?-tetraalkylamide of diglycolic acid, and a solution for back extraction is a composition of 5-20 g/l of a complexone, 5-60 g/l of a nitrogen-containing organic acid and 60-240 g/l of a salting-out agent.

Abstract: Techniques for forming an enclosure comprised of aluminum zirconium alloy layer are disclosed. In some embodiments, aluminum ions and zirconium ions can be dissolved in a non-aqueous ionic liquid in an electrolytic plating bath. A reverse pulsed electric current can facilitate in co-depositing the aluminum ions and the zirconium ions onto a metal substrate. The resulting aluminum zirconium alloy layer can include nanocrystalline grain structures, which can impart the alloy layer with increased hardness and increased resistance to scratching, denting, and abrasion. In some embodiments, the aluminum zirconium alloy layer can be anodized to form an aluminum oxide layer. Subsequent to the anodization operation, the oxidized layer is able to retain its substantially neutral color.

Abstract: New 6xxx aluminum alloys are disclosed. The new 6xxx aluminum alloys may include 1.05-1.50 wt. Mg, 0.60-0.95 wt. % Si, where the (wt. % Mg)/(wt. % Si) is from 1.30 to 1.90, 0.275-0.50 wt. % Cu, and from 0.05 to 1.0 wt. % of at least one secondary element, wherein the secondary element is selected from the group consisting of V, Fe, Cr, Mn, Zr, Ti, and combinations thereof.

Abstract: A vanadium alloy essentially consisting of: vanadium; and aluminium having a content of greater than 0 to 10 at %, and a process of producing thereof.

Abstract: Disclosed herein is a shape memory alloy comprising 48 to 50 atomic percent nickel, 15 to 30 atomic percent hafnium, 1 to 5 atomic percent aluminum; with the remainder being titanium. Disclosed herein too is a method of manufacturing a shape memory alloy comprising mixing together to form an alloy nickel, hafnium, aluminum and titanium in amounts of 48 to 50 atomic percent nickel, 15 to 30 atomic percent hafnium, 1 to 5 atomic percent aluminum; with the remainder being titanium; solution treating the alloy at a temperature of 700 to 1300° C. for 50 to 200 hours; and aging the alloy at a temperature of 400 to 800° C. for a time period of 50 to 200 hours to form a shape memory alloy.

Abstract: A high strength cast aluminium alloy for high pressure die casting comprising magnesium silicide 6 to 12 wt. %, magnesium 4 to 10 wt. %, X element from copper (Cu), zinc (Zn), silver (Ag), gold (Au) and Lithium (Li) at 3 to 10 wt. %, manganese 0.1 to 1.2 wt. %, iron max. 1.5 wt. %, titanium or the other grain refining elements from Cr, Nb, and Sc with 0.02 to 0.4 wt. %, and impurity and minor alloying elements at a level of maximum 0.3 wt. % and totally <0.5% of at least one element selected from scandium (Sc), zirconium (Zr), Nickel (Ni), chromium (Cr), niobium (Nb), gadolinium (Gd), calcium (Ca), yttrium (Y), antinomy (Sb), bismuth (Bi), neodymium (Nd), ytterbium (Yb), vanadium (V), chromium (Cr), beryllium (Be) and boron (B) and the remainder aluminium.

Abstract: The present invention provides a superelastic alloy formed by addition of Fe or Co to an Au—Cu—Al alloy, including: Cu of 12.5% by mass or more and 16.5% by mass or less; Al of 3.0% by mass or more and 5.5% by mass or less; Fe or Co of 0.01% by mass or more and 2.0% by mass or less; and a balance Au, and a difference between Al content and Cu content (Cu—Al) is 12% by mass or less. The superelastic alloy according to the present invention has superelastic property while being Ni-free, excellent X-ray imaging property, processability, and strength property, and is suitable for a medical field.

Abstract: Described is a TiAl alloy which, besides titanium, comprises 42 to 48 at. % aluminum, 3 to 5 at. % niobium, 0.05 to 1 at. % molybdenum, 0.2 to 2.2 at. % silicon, 0.2 to 0.4 at. % carbon, 0.05 to 0.2 at. % boron, and optionally tungsten, zirconium and hafnium, as well as unavoidable impurities, and at room temperature has a microstructure which comprises globular colonies of lamellae of ?2-Ti3Al and ?-TiAl, as well as silicide precipitates, and essentially no ? phase. A method for producing a component made of this alloy is also described.

Abstract: Steel strip provided with a hot dip galvanized zinc alloy coating layer, in which the coating of the steel strip is carried out in a bath of molten zinc alloy, the zinc alloy in the coating of: 0.3-2.3 weight % magnesium; 0.6-2.3 weight % aluminium; optional <0.2 weight % of one or more additional elements; unavoidable impurities; the remainder being zinc in which the zinc alloy coating layer has a thickness of 3-12 ?m.

Abstract: An Al—Fe alloy coated steel sheet includes a base steel sheet and an alloy coating layer, wherein the base steel sheet includes, by wt %, C: 0.1%˜0.5%, Si: 0.01%˜2%, Mn: 0.01%˜10%, P: 0.001%˜0.05%, S: 0.0001%˜0.02%, Al: 0.001%˜1.0%, N: 0.001%˜0.02%, and the balance of Fe and other impurities, wherein the alloy coating layer includes: an Al—Fe alloy layer I formed on the base steel sheet and having a Vickers hardness of 200˜800 Hv; an Al—Fe alloy layer III formed on the Al—Fe alloy layer I and having a Vickers hardness of 700˜1200 Hv; and an Al—Fe alloy layer II formed in the Al—Fe alloy layer III continuously or discontinuously in a length direction of the steel sheet, and having a Vickers hardness of 400˜900 Hv, wherein an average oxygen content at a depth of 0.1 ?m from a surface of the oxide layer is 20% or less by weight.

Abstract: An air seal for use in a gas turbine engine. The seal includes a thermally sprayed abradable seal layer. The abradable material is composed of aluminum powder forming a metal matrix, and co-deposited methyl methacrylate particles and/or hexagonal boron nitride particles embedded as filler in the metal matrix.

Abstract: Provided herein is an alloy steel in which carburization is prevented by a processing load, the alloy steel including: about 0.13 to 0.25 wt % of carbon (C), about 0.6 to 1.5 wt % of silicon (Si), about 0.6 to 1.5 wt % of manganese (Mn), about 1.5 to 3.0 wt % of chromium (Cr), about 0.01 to 0.1 wt % of niobium (Nb), about 0.01 to 0.1 wt % of aluminum (Al), about 0.05 to 0.5 wt % of vanadium (V), the balance iron (Fe), and impurities, based on the total weight of the alloy steel.