Abstract: The present invention describes a packing with a three-dimensional structure which is particularly suited to creating homogenous and anisotropic mixing between a gas phase and a dispersed solid phase moving in counter-current flow. Application of this packing to the stripper of fluidized catalytic cracking units.

Abstract: Treatment of hydrocarbon streams, and in one non-limiting embodiment refinery distillates, with reducing agents, such as borohydride and salts thereof, alone or together with at least one co-solvent results in reduction of the sulfur compounds such as disulfides, mercaptans, thiophenes, and thioethers that are present to give easily removed sulfides. In one non-limiting embodiment, the treatment converts the original sulfur compounds into hydrogen sulfide or low molecular weight mercaptans that can be extracted from the distillate with caustic solutions, hydrogen sulfide or mercaptan scavengers, solid absorbents such as clay or activated carbon or liquid absorbents such as amine-aldehyde condensates and/or aqueous aldehydes.

Abstract: Catalyst composition and parameters for catalytic hydrothermal liquefaction of biomass to bio-oil fuels target municipal solid waste (MSW) rather than lignin rich plant waste typically sought for bio-oil production. An HTL (Hydrothermal Liquefaction) reactor generates bio-fuel from municipal solid waste (MSW), including receiving, in the HTL reactor, non-lignin based waste from a municipal processing stream, and adding a solvent for extracting sugars from green waste components of the municipal processing stream. The reactor extracts a liquid from the received waste, and converts water soluble products in the liquid into oil soluble products via C—C(carbon-to-carbon) coupling reactions. A catalyst added to the resulting aqueous stream recovers a beneficial oil product, and is optimized by tuning acid and base sites on the solid catalyst.

Abstract: Processes and apparatuses are disclosed for treating a naphtha boiling range stream containing mercaptan compounds. The process includes oxidizing mercaptan compounds in the naphtha boiling range stream to provide a mercaptan-depleted naphtha stream rich in disulfide compounds; passing the mercaptan-depleted naphtha stream rich in disulfide compounds to a naphtha splitter column; and fractionating at least a portion of the mercaptan-depleted naphtha stream rich in disulfide compounds into at least two streams, a light naphtha stream lean in disulfide compounds and a heavy naphtha stream rich in disulfide compounds. The heavy naphtha stream rich in disulfide compounds may be passed to a hydroprocessing unit to convert organic disulfides in the stream to hydrocarbons and hydrogen sulfide. A heavy naphtha stream lean in disulfide compounds can be recovered and routed as desired by the refiner.

Abstract: Process scheme configurations are disclosed that enable conversion of crude oil feeds with several processing units in an integrated manner into petrochemicals. The designs utilize minimum capital expenditures to prepare suitable feedstocks for the steam cracker complex. The integrated process for converting crude oil to petrochemical products including olefins and aromatics, and fuel products, includes mixed feed steam cracking and gas oil steam cracking. Feeds to the mixed feed steam cracker include light products and naphtha from hydroprocessing zones within the battery limits, recycle streams from the C3 and C4 olefins recovery steps, and raffinate from a pyrolysis gasoline aromatics extraction zone within the battery limits. Feeds to the gas oil steam cracker include unconverted oil intermediates from vacuum gas oil hydrotreating.

Abstract: A process for converting C2-5 alkanes to higher value C5-24 hydrocarbon fuels and blendstocks. The C2-5 alkanes are converted to olefins by thermal olefination, without the use of a dehydrogenation catalyst and without the use of steam. The product olefins are fed to an oligomerization reactor containing a zeolite catalyst to crack, oligomerize and cyclize the olens to the fuel products which are then recovered. Optionally, hydrogen and methane are removed from the product olefin stream prior to oligomerization. Further optionally, C2-5 alkanes are removed from the product olefin stream prior to oligomerization.

Abstract: A stream of cracked naphtha is fractionated into at least four specified fractions defined by their respective boiling point ranges. The lightest fraction, IBP to 50° C., is treated in a selective etherification or alkylation process to reduce its RVP value and increase its RON. The second fraction, 50° C. to 150° C., is selectively hydrogenated to treat and convert the diolefins present and the treated stream is sent directly to the gasoline blending pool since it has the desired RON and low sulfur content. The third, and optionally a fourth fraction, boiling in the range of 50° C. to 180° C., in an embodiment, are utilized for the production of aromatics and the raffinate stream, after aromatic extraction, is sent to the gasoline blending pool. A fraction of this latter stream can optionally be recycled for further cracking to produce additional aromatics and gasoline blending components. The heaviest fraction, 180° C.

Abstract: The present disclosure relates to systems operable to catalytically convert a hydrocarbon feed stream predominantly comprising both isopentane and n-pentane to yield upgraded hydrocarbon products that are suitable for use either as a blend component of liquid transportation fuels or as an intermediate in the production of other value-added chemicals. The hydrocarbon feed stream is isomerized in a first reaction zone to convert at least a portion of the n-pentane to isopentane, followed by catalytic-activation of the isomerization effluent in a second reaction zone with an activation catalyst to produce an activation effluent. The process increases the conversion of the hydrocarbon feed stream to olefins and aromatics, while minimizing the production of C1-C4 light paraffins. Certain embodiments provide for further upgrading of at least a portion of the activation effluent by either oligomerization or alkylation.

Abstract: A downdraft gasifier and method of gasification with high yield biochar that utilizes a plurality of high throughput, vertically positioned tubes to create a pyrolysis zone, an oxidation zone beneath the pyrolysis zone and a reduction zone beneath the oxidation zone. A rotating and vertically adjustable rotating grate is located beneath the reduction zone of the gasifier. In addition, a drying zone is located above the pyrolysis zone so the heat of the gasifier can be used to dry feedstock before it enters the gasifier. By optimizing the grate height and rpm, feedstock retention time in the drying zone, the drying zone temperature and feedstock moisture content, the result is gasification of biomass with a high yield and continuous biochar production.

Abstract: Fuel and lubricant compositions are provided that contain an organic hydride-based reductant. Methods for preventing or reducing low speed pre-ignition events in an internal combustion engines using these compositions are also provided.

Abstract: A grease composition includes base oil, a thickener, and an organic modified polysiloxane. The base oil is an alkyl diphenyl ether. The thickener is a urea thickener, and the grease composition contains 10 to 30 mass % of the thickener based on the total amount of the base oil and the thickener. The grease composition contains 0.2 to 10 mass % of the organic modified polysiloxane based on the total amount of the base oil and the thickener.

Abstract: The invention discloses a lubricating grease with thixotropy and a preparation method thereof. The lubricating grease comprises a polyurea/high base number calcium sulfonate composite thickener, colloidally dispersed calcium carbonate solid particles in the form of calcite, a calcium borate or calcium borate composite, and a calcium soap of fatty acid having 12 to 24 carbon atoms. The above components are uniformly dispersed in an oil phase medium. The content of high base number calcium sulfonate in the lubricating grease is less than 22 wt %, and the lubricating grease has a worked cone penetration of 295 or less.

Abstract: The present technology generally relates to fragrance compositions comprising essential oils and products comprising the same. The present technology also relates to methods of using the fragrance compositions to reduce fatigue and improve executive brain function.

Abstract: The present technology relates to fragrance compositions comprising essential oils and products comprising the same. The technology also relates to methods of using the fragrance compositions to induce sleep and promote wellness.

Abstract: Trans isomers of formula (I) in the form of any of its stereoisomers or a mixture thereof are provided, wherein the bold and hatched lines indicate a relative or absolute configuration. Also provided are their uses as perfuming ingredients to impart vetiver/rooty notes e.g. in perfuming compositions or in consumer products.

Abstract: A gel-type scented candle includes a container, a wick attached to and erected at the center of the inner bottom of the container, and a candle oil accommodated in the container, in which the candle oil is of a gel type made by adding a natural vegetable oil with a natural perfume and a natural pigment, and the container can be reused by being refilled with the candle oil by a user before or after use of the candle oil.

Abstract: Detergent compositions and more specifically, to low pH detergent compositions comprising sulfated surfactants, organic acid, and polyamine compounds. Methods of making and using the same.

Abstract: The need for a liquid hand-dishwashing composition which provides further improved sudsing volume and longevity when washing dishware using diluted liquid hand dishwashing compositions, especially in the presence of greasy soil and particulate soil, while still providing the desired cleaning, is met when the composition is formulated with a surfactant system comprising a specific level of alkyl sulfate surfactant and a co-surfactant, when the alkyl chain of the alkyl sulfate anionic surfactant has a mol fraction of C12 and C13 chains of at least about 50%, and the C13/C12 mol ratio of the alkyl chain is at least about 57/43.

Abstract: The present invention relates to polypeptides, and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.

Abstract: The invention relates to polypeptides having hydrolytic activity and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.

Abstract: The present invention relates to polypeptides comprising a GH39 glycosyl hydrolase domain and polynucleotides encoding the polypeptides. The invention further relates to compositions comprising such polypeptides such as cleaning compositions, use of polypeptides comprising the GH39 domain in cleaning processes. The invention further relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.

Abstract: This present disclosure relates to novel detergent compositions comprising bacterial mannanase enzymes. The detergent compositions comprising bacterial mannanases are useful in laundry and cleaning applications wherein degradation or modification of mannan is desired. The present disclosure also relates to the use of said detergent compositions in laundry and cleaning applications as well as a method for degrading mannan.

Abstract: According to an aspect of the present disclosure, a method of treating a textile with an antimicrobial agent over a plurality of laundry cycles each including a wash cycle and a treatment cycle. The method includes (a) receiving a textile in a wash system for a first laundry cycle, (b) initiating a wash cycle comprising a detergent, (c) initiating a post-detergent treatment cycle comprising dosing the textile with a solution having a predetermined concentration of an antimicrobial agent that comprises a metallic ion, and (c) repeating steps (a)-(c) for each of a plurality of additional laundry cycles. The predetermined concentration is insufficient to achieve a predetermined antimicrobial efficacy for the textile due to the first laundry cycle alone but sufficient to achieve the predetermined antimicrobial efficacy for the textile due to a combination of the first laundry cycle and one or more of the plurality of additional laundry cycles.

Abstract: The invention relates to the use of a cleaning composition for removing uncured printing resin from 3D-printed articles, the cleaning composition comprising either of the following components alone or in combination: di basic esters of a carboxylic acid, tri basic esters of a carboxylic acid. The invention also relates to a process of cleaning a 3D-printed article, the process comprising the steps of a) providing the cleaning composition and a 3D-printed article comprising uncured printing resin on its surface, b) treating the surface of the 3D-printed article with the cleaning composition, c) optionally treating the 3D article with a solvent, in particular water, d) optionally drying the 3D article, optionally repeating steps b), c) and d) either singly or in combination.

Abstract: A single dose pack exhibiting improved enzyme stability and methods for producing the same are provided. The single dose pack includes a container composed of a water-soluble film and a wash composition encapsulated within the container. The wash composition includes a detergent surfactant, an enzyme, and a solvent blend. The enzyme is present in an amount of about 0.01 wt. % to about 0.5 wt. % active enzyme, based on the overall weight of the wash composition. The solvent blend includes water; a non-aqueous solvent selected from the group consisting of propylene glycol, glycerin, polyethylene glycol, and mixtures of two or more thereof; and a saccharide system including fructose and glucose. The saccharide system is present in an amount of about 1 wt. % to about 30 wt. %, based on the overall weight of the wash composition.

Abstract: The present invention relates to a new yeast product that is suitable for the production of beer, and uses of this product. The product is a closed container containing 0.1 to 50 l of a frozen yeast product wherein the frozen yeast product contains at least one strain, e.g. one, two or three yeast strains, suitable for beer brewing in a total concentration of at least 109 CFU/g, with each yeast strain suitable for beer brewing being present in a concentration of at least 108 CFU/g, the frozen yeast product provides a liquid aqueous suspension with a dry matter content below 35% (w/w) upon thawing; and no cryoprotectant is added to the frozen yeast product.

Abstract: Wine-making process comprising a first step of arranging “Acacia Verek” raw gum arabic and a second step of arranging a wine to be bottled, a step of dissolving the raw gum arabic in water in order to obtain raw gum arabic in solution with a concentration of gum arabic comprised between 5 and 50%, a step of filtering the raw gum arabic in solution by means of a tangential filter provided with synthetic membrane with nominal porosity comprised between 0.5 and 3 ?m in order to obtain filtered gum arabic in solution with a concentration of gum arabic comprised between 5 and 40%, a step of admixing the filtered gum arabic in solution in the wine to be bottled in order to obtain a wine to be bottled admixed with gum arabic, and finally a step of microfiltrating the wine to be bottled admixed with gum arabic.

Abstract: The present invention relates to a method for cultivating cells, in particular tissues, comprising a carrier plate unit, which has at least one access opening, at least one cultivation chamber, and at least one channel connecting the access opening to the cultivation chamber.

Abstract: Provided herein are methods and devices for measuring and monitoring proliferation and toxicity in vitro.

Abstract: An aqueous cell culture medium composition includes an aqueous cell culture solution configured to support the culture of mammalian cells. The composition further includes a synthetic polymer conjugated to a polypeptide dissolved in the aqueous cell culture solution. The synthetic polymer conjugated to a polypeptide is configured to attach to the surface of a cell culture article under cell culture conditions. Incubation of the aqueous cell culture medium composition on a cell culture surface under cell culture conditions results is attachment to the surface of the synthetic polymer conjugated to the polypeptide.

Abstract: Disclosed herein are methods of genome-editing and transduction of T cells and methods of immunotherapy in using them. In particular, the disclosure relates to engineered chimeric antigen receptor (CAR)-bearing T cells and methods of using the same for the treatment of cancer.

Abstract: The invention provides improved T cell compositions and methods for manufacturing T cells. More particularly, the invention provides methods of T cell manufacturing that result in adoptive T cell immunotherapies with improved survival, expansion, and persistence in vivo.

Abstract: Cancer immunotherapy using genetically engineered NK cells is enhanced by expression of recombinant co-stimulatory molecules to deliver co-stimulatory signals to a recipient host's immune cells to enhance an immune response.

Abstract: Disclosed is a cell-derived extracellular matrix (ECM) derived in vitro from cells isolated from amniotic fluid, and methods of use for the isolation, maintenance, and proliferation of adherent cells including stem cells, as well as for the differentiation of stem cells.

Abstract: A method for preparing differentiation-induced cells from embryoid bodies derived from pluripotent stem cells is provided. The method includes adding a protease to embryoid bodies. The protease disperses the embryoid bodies, and the protease has an enzyme activity in the range from 0.3 to 4.0 recombinant protease activity unit (rPU)/ml.

Abstract: The present invention discloses an innovative preparation process for recombinant human fat cell peptide (BMP-2 stem cells for short), comprising the following steps: gene synthesis of BMP-2 stem cells, specific expression system construction of mature peptide, expression induction, separation, purification and amplification. The method disclosed by the present invention is simple and flexible; has simple product separation and purification process, high purity, high activity and low production cost; has the functions of preventing clinical virus, resisting cell aging, and promoting wound healing and repair in epidermis, dermis and mucosa; and has broad application prospects in the fields such as medical care, cosmetology and health care.

Abstract: Disclosed is a three-dimensional (3D) cell cluster comprising transdifferentiated adult mammalian non-pancreatic beta cells having a mature pancreatic beta cell phenotype and function and a scaffold, wherein said transdifferentiated cells have an enhanced mature pancreatic beta cell phenotype compared to a 3D cell cluster without a scaffold and to similarly transdifferentiated cells cultured as a two-dimensional (2D) monolayer.

Abstract: The invention disclosed herein generally relates to methods and systems for converting stem cells into specific tissue(s) or organ(s) through directed differentiation. In particular, the invention disclosed herein relates to methods and systems for promoting definitive endoderm formation from pluripotent stem cells.

Abstract: Provided herein are compositions and methods for generating visible light photopolymerized hydrogels to support cell viability, expansion, and encapsulation. The present disclosure provides a composition, comprising a visible light harvesting complex, a photoinitiator, a co-initiator, a di-thiol-terminated crosslinker, and at least one cysteine-containing peptide. The present disclosure provides a method of generating a visible light photopolymerized hydrogel. In further embodiments that method comprises generating a 3-dimensional endothelial network comprising the visible light photopolymerized hydrogel. In additional embodiments the method comprises generating a hydrogel network comprising the visible light photopolymerized hydrogel comprising at least one cell.

Abstract: The present disclosure is intended to reduce the amount of glycerin produced as a byproduct in ethanol fermentation to a significant extent using a transgenic yeast comprising a gene having the pentose assimilating ability and encoding glycerin dehydrogenase having a mitochondrial transport signal introduced thereinto.

Abstract: The disclosure relates to variant carboxylic acid reductase (CAR) enzymes for the improved production of fatty alcohols in recombinant host cells.

Abstract: This invention provides a range of translatable polynucleotide and oligomer molecules for expressing a human phenylalanine hydroxylase (PAH), or a fragment thereof having PAH activity. The polynucleotide and oligomer molecules are expressible to provide the human PAH or a fragment thereof having PAH activity. The molecules can be used as active agents to express an active polypeptide or protein in cells or subjects. The agents can be used in methods for ameliorating, preventing, delaying onset, or treating a disease or condition associated with phenylketonuria, decreased metabolism of phenylalanine, or increased levels of phenylalanine in a subject.

Abstract: The present invention relates to non-naturally occurring polypeptides useful for preparing armodafinil, polynucleotides encoding the polypeptides, and methods of using the polypeptides. The non-naturally occurring polypeptides of the present invention are effective in carrying out biocatalytic conversion of the (i) 2-(benzhydrylsulfinyl)acetamide to (?)-2-[(R)-(diphenylmethyl)sulfinyl]acetamide (armodafinil), or (ii) benzhydryl-thioacetic acid to (R)-2-(benzhydrylsulfinyl)acetic acid, which is a pivotal intermediate in the synthesis of armodafinil, in enantiomeric excess.

Abstract: Disclosed are methods and compositions for increasing the triacylglycerol content of a cell by up-regulating diacylglycerol acyltransferase and down-regulating triacylglycerol lipase. In some embodiments, a DGA1 protein is expressed and a native TGL3 gene is knocked out, thereby increasing the synthesis of triacylglycerol and decreasing its consumption, respectively.

Abstract: The present invention provides engineered transaminase polypeptides useful for the synthesis of chiral amine compounds under industrially relevant conditions. The invention also provides polynucleotides encoding the engineered transaminase polypeptides, host cells capable of expressing the engineered transaminases, and methods of using the engineered transaminases for the production of chiral amine compounds.

Abstract: The present disclosure provides engineered transaminase polypeptides for the production of amines, polynucleotides encoding the engineered transaminases, host cells capable of expressing the engineered transaminases, and methods of using the engineered transaminases to prepare compounds useful in the production of active pharmaceutical agents.

Abstract: The present disclosure provides engineered transaminase enzymes having improved properties as compared to a naturally occurring wild-type transaminase enzyme. Also provided are polynucleotides encoding the engineered transaminase enzymes, host cells capable of expressing the engineered transaminase enzymes, and methods of using the engineered transaminase enzymes to synthesize a variety of chiral compounds.

Abstract: The present invention relates to polypeptides having DNase activity and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.

Abstract: The present disclosure concerns polynucleotides and amino acids of Acidaminococcus sp. Cas12a (Cpf1) and methods for their use for genome editing in eukaryotic cells.

Abstract: Rationally-designed LAGLIDADG meganucleases and methods of making such meganucleases are provided. In addition, methods are provided for using the meganucleases to generate recombinant cells and organisms having a desired DNA sequence inserted into a limited number of loci within the genome, as well as methods of gene therapy, for treatment of pathogenic infections, and for in vitro applications in diagnostics and research.