Patents Issued in July 2, 2020
  • MODIFIED UROKINASE-TYPE PLASMINOGEN ACTIVATOR POLYPEPTIDES AND METHODS OF USE
    Publication number: 20200208133
    Abstract: Provided are u-PA polypeptides and fusion proteins containing the u-PA polypeptides. The u-PA polypeptides are modified to have altered activity and/or specificity so that they cleave a complement protein, such as complement protein C3, to thereby inhibit complement activation. The modified u-PA polypeptides and fusion proteins that inhibit complement activation can be used for treatment of diseases and conditions that are mediated by complement activation, or in which complement activation plays a role. These disorders include ischemic and reperfusion disorders, including myocardial infarction and stroke, sepsis, autoimmune diseases, diabetic retinopathies, age-related macular degeneration, transplanted organ rejection, inflammatory diseases and diseases with an inflammatory component.
    Type: Application
    Filed: January 3, 2020
    Publication date: July 2, 2020
    Inventors: Edwin L. Madison, Christopher Thanos, Vanessa Soros, Mikhail Popkov, Kimberly Tipton, Matthew John Traylor, Eric Steven Furfine, Jeffrey Charles Way
  • MEANS AND METHODS FOR PRODUCING PHOSPHATE CONTAINING CAPSULAR POLYSACCHARIDES
    Publication number: 20200208134
    Abstract: The present invention relates to a host cell, which comprises under the control of a heterologous promoter a polynucleotide comprising a nucleotide sequence encoding a polypeptide capable of synthesizing a polysaccharide consisting of a dimeric repeating unit as well as to a vaccine composition comprising such host cell. Furthermore, either such host cell or a polypeptide expressed by such host cell is used for the production of a polysaccharide consisting of a dimeric repeating unit which may be used as a glycoconjugate vaccine.
    Type: Application
    Filed: July 26, 2018
    Publication date: July 2, 2020
    Inventors: Timm Fiebig, Christa Litschko, Rita Gerardy-Schahn, Andrea Bethe, Monika Berger
  • USING NUCLEOSOME INTERACTING PROTEIN DOMAINS TO ENHANCE TARGETED GENOME MODIFICATION
    Publication number: 20200208135
    Abstract: Compositions and methods for using nucleosome interacting protein domains to increase accessibility of programmable DNA modification proteins to target chromosomal sequences, thereby increasing efficiency of targeted genome/epigenetic modification in eukaryotic cells.
    Type: Application
    Filed: February 18, 2020
    Publication date: July 2, 2020
    Inventors: Fuqiang Chen, Xiao Ding, Yongmei Feng, Gregory Davis
  • METHOD OF ISOLATING NUCLEIC ACID
    Publication number: 20200208136
    Abstract: Disclosed herein is a method of isolating nucleic acid from a sample containing nucleic acid, the method comprising (a) exposing the sample to a thermoplastic polymer substrate under conditions that allow nucleic acid in the sample to reversibly bind to the substrate; (b) washing the nucleic acid-bound substrate of (a) under conditions that preferentially remove non-nucleic acid impurities bound to the substrate; and (c) exposing the washed nucleic acid-bound substrate of (b) to an elution buffer, thereby recovering the nucleic acid from the substrate.
    Type: Application
    Filed: July 25, 2018
    Publication date: July 2, 2020
    Inventors: Eugene J. H. Wee, Will Anderson, Yadveer Singh Grewal
  • Method for Using Aqueous Two-Phase System for the Isolation, Purification and/or Concentration of Short Nucleic Acid Fragments
    Publication number: 20200208137
    Abstract: The present invention relates to methods of using aqueous two-phase system (ATPS) for the isolation, concentration and/or purification of short nucleic acid fragment having about or less than 250 base pairs (bp). In one embodiment, the present invention provides a composition and kit for the purification of short nucleic acid fragments having about or less than 250 base pairs from nucleic acid-containing biological materials. In another embodiment, the present invention provides uses of certain salts and/or polymers in a two-phase system for the purification of short nucleic acid fragments having about or less than 250 base pairs from nucleic acid containing biological materials.
    Type: Application
    Filed: September 17, 2018
    Publication date: July 2, 2020
    Inventor: Yin To CHIU
  • GENOMIC SEQUENCE MODIFICATION METHOD FOR SPECIFICALLY CONVERTING NUCLEIC ACID BASES OF TARGETED DNA SEQUENCE, AND MOLECULAR COMPLEX FOR USE IN SAME
    Publication number: 20200208138
    Abstract: The invention provides a method of modifying a targeted site of a double stranded DNA, including a step of contacting a complex wherein a nucleic acid sequence-recognizing module that specifically binds to a target nucleotide sequence in a selected double stranded DNA and a nucleic acid base converting enzyme are linked, with the double stranded DNA, to convert one or more nucleotides in the targeted site to other one or more nucleotides or delete one or more nucleotides, or insert one or more nucleotides into the targeted site, without cleaving at least one strand of the double stranded DNA in the targeted site.
    Type: Application
    Filed: February 14, 2020
    Publication date: July 2, 2020
    Inventors: Keiji Nishida, Akihiko Kondo, Satomi Kojima
  • METHODS FOR NUCLEIC ACID ASSEMBLY AND HIGH THROUGHPUT SEQUENCING
    Publication number: 20200208139
    Abstract: Methods and apparatus of some aspects of the invention relate to the synthesis of high fidelity polynucleotides. In particular, aspects of the invention relate to concurrent enzymatic removal of amplification sequences and ligation of processed oligonucleotides into nucleic acid assemblies. According to some embodiments, the invention provides a method for producing a target nucleic acid having a predefined sequence. In some embodiments, the method comprises the step of providing a plurality of oligonucleotides, wherein each oligonucleotides comprises (i) an internal sequence identical to a different portion of a sequence of a target nucleic acid, (ii) a 5? sequence flanking the 5? end of the internal sequence and a 3? flanking sequence flanking the 3? end of the internal sequence, each of the flanking sequence comprising a primer recognition site for a primer pair and a restriction enzyme recognition site.
    Type: Application
    Filed: December 13, 2019
    Publication date: July 2, 2020
    Applicant: Gen9, Inc.
    Inventors: Michael E. Hudson, Li-Yun A. Kung, Daniel Schindler, Stephen Archer, Ishtiaq Saaem
  • METHODS OF MAKING AND USING TANDEM, TWIN BARCODE MOLECULES
    Publication number: 20200208140
    Abstract: Disclosed herein are methods related to the production of tandem, twin barcode (TTB) molecules. These TTB molecules are useful in sequencing to identify and resolve errors.
    Type: Application
    Filed: August 31, 2018
    Publication date: July 2, 2020
    Inventors: Sanggu KIM, Hannah YU, Alice BAEK
  • METHODS AND COMPOSITIONS COMPRISING CRISPR-CPF1 AND PAIRED GUIDE CRISPR RNAS FOR PROGRAMMABLE GENOMIC DELETIONS
    Publication number: 20200208141
    Abstract: Described are methods comprises transducing a mammalian cell with one or more virus vectors. Each vector comprises a nucleic acid sequence encoding a Cpf1 (also known as Cas12a) protein and an optional selectable marker in operative association with an RNA pol II promoter which controls expression thereof; and a CRISPR RNA (crRNA) array comprising at least two spacers in operative association with an RNA pol III promoter. Each spacer encodes an RNA guide which hybridizes to a unique sequence located 3? from a T-rich protospacer-adjacent motif (PAM) in a genomic region of interest. The method further comprises culturing the transduced cells, thereby providing a plurality of cultured cell cultures, each cell culture comprising said deletion. Additionally, described are compositions used in methods as well as libraries generated by the methods. Such compositions comprise libraries of transduced cell cultures, viral vectors, nucleic acid sequences, CRISPR RNA spacers, and RNA guides, as described herein.
    Type: Application
    Filed: March 2, 2020
    Publication date: July 2, 2020
    Applicants: NEW YORK GENOME CENTER, INC., NEW YORK UNIVERSITY
    Inventor: Neville Espi SANJANA
  • USE OF BIOLOGICAL RNA SCAFFOLDS WITH IN VITRO SELECTION TO GENERATE ROBUST SMALL MOLECULE BINDING APTAMERS FOR GENETICALLY ENCODABLE BIOSENSORS
    Publication number: 20200208142
    Abstract: Provided herein are libraries of scaffolds derived from riboswitches and small ribozymes and their methods of use. The scaffolds of the invention yield aptamers that are easily identified and characterized by virtue of the structural scaffold. The nature of the scaffold predisposes these RNAs for coupling to readout domains to engineer biosensors that function in vitro and in vivo. Biosensors, synthetic RNA agents and synthetic DNA agents, and their methods of use, are also provided.
    Type: Application
    Filed: December 11, 2017
    Publication date: July 2, 2020
    Inventors: Robert T. Batey, Ely B. Porter
  • METHODS AND COMPOSITIONS FOR AMPLIFYING SHORT DNA FRAGMENTS
    Publication number: 20200208143
    Abstract: Methods, compositions and systems to amplify short DNA fragments, such as cfDNA, and to reduce random base errors formed in template-dependent primer extension reactions, and to find variant frequencies of mutations on the DNA fragments. The methods, compositions and systems described herein may include, or include the use of, poly(dA) tailing of short DNA fragments by a terminal deoxynucleotidyl transferase, linearly amplification and a multiplex primer extension reaction.
    Type: Application
    Filed: December 12, 2019
    Publication date: July 2, 2020
    Inventors: Zhitong LIU, Chenyu LI, Lucie LEE, Yang Lily LIU, Jeffrey Juehui LIU
  • METHODS AND COMPOSITIONS USING ONE-SIDED TRANSPOSITION
    Publication number: 20200208144
    Abstract: Embodiments provided herein relate to methods and compositions for next generation sequencing. Some embodiments include the preparation of a template library from a target nucleic acid using one-sided transposition, sequencing the template library, and capturing the contiguity information.
    Type: Application
    Filed: January 7, 2020
    Publication date: July 2, 2020
    Inventors: Frank J. Steemers, Jeffrey S. Fisher, Kevin L. Gunderson, Sasan Amini, Christian Gloeckner
  • MODIFIED MESSENGER RNA COMPRISING FUNCTIONAL RNA ELEMENTS
    Publication number: 20200208145
    Abstract: The present disclosure provides messenger RNAs (mRNAs) having chemical and/or structural modifications, including RNA elements and/or modified nucleotides, which provide a desired translational regulatory activity to the mRNA.
    Type: Application
    Filed: May 18, 2018
    Publication date: July 2, 2020
    Applicant: ModernaTX, Inc.
    Inventors: Melissa J. MOORE, Caroline KÖHRER, Ruchi JAIN, Vladimir PRESNYAK
  • MATERIALS AND METHODS FOR EFFICIENT TARGETED KNOCK IN OR GENE REPLACEMENT
    Publication number: 20200208146
    Abstract: Materials and methods for generating targeted knock ins and gene replacements with high precision and efficiency are provided herein.
    Type: Application
    Filed: July 12, 2018
    Publication date: July 2, 2020
    Inventors: Karl J. Clark, Stephen C. Ekker, Jeffrey Essner, Jordan Michael Welker, Maira Pedroso de Almeida, Wesley Allen Wierson, Maura McGrail
  • MODULATORS OF IRF5 EXPRESSION
    Publication number: 20200208147
    Abstract: The present embodiments provide methods, compounds, and compositions useful for inhibiting IRF5 expression, which may be useful for treating, preventing, or ameliorating a disease associated with IRF5.
    Type: Application
    Filed: November 15, 2019
    Publication date: July 2, 2020
    Inventor: Susan M. FREIER
  • EUGLOBULIN-BASED METHOD FOR DETERMINING THE BIOLOGICAL ACTIVITY OF DEFIBROTIDE
    Publication number: 20200208148
    Abstract: It is disclosed a method for determining the biological activity of defibrotide, which comprises the steps of: a) bringing into contact defibrotide, mammalian euglobulin and a substrate specific for the plasmin which, by reaction with the plasmin, provides a measurable product; and b) measuring the amount of product formed at successive times, to thereby determine the biological activity of the defibrotide. Liquid defibrotide formulations are also disclosed, preferably water solutions, having a defined biological activity and, in particular, having an activity of 25 to 35 IU/mg of defibrotide, preferably from 27 to 32 IU/mg and, more preferably, from 28 to 32 IU/mg.
    Type: Application
    Filed: March 12, 2020
    Publication date: July 2, 2020
    Inventors: Terenzio IGNONI, Vijay KUMAR, Khalid ISLAM
  • Alpha-1 AntiTrypsin (AAT) RNAi Agents, Compositions Including AAT RNAi Agents, And Methods of Use
    Publication number: 20200208149
    Abstract: RNAi agents for inhibiting the expression of the alpha-1 antitrypsin (AAT) gene, compositions including AAT RNAi agents, and methods of use are described. Also disclosed are pharmaceutical compositions including one or more AAT RNAi agents together with one or more excipients capable of delivering the RNAi agent(s) to a liver cell in vivo. Delivery of the AAT RNAi agent(s) to liver cells in vivo inhibits AAT gene expression and treats diseases associated with AAT deficiency such as chronic hepatitis, cirrhosis, hepatocellular carcinoma, transaminitis, cholestasis, fibrosis, and fulminant hepatic failure.
    Type: Application
    Filed: September 12, 2019
    Publication date: July 2, 2020
    Inventors: Zhen Li, Rui Zhu, Christine I. Wooddell, Tao Pei
  • METHODS FOR TREATING OR PREVENTING CONTACT-ACTIVATION PATHWAY-ASSOCIATED DISEASES USING iRNA COMPOSITIONS TARGETING FACTOR XII (HAGEMAN FACTOR) (F12)
    Publication number: 20200208150
    Abstract: The present invention relates to methods of use of RNAi agents, e.g., double stranded RNAi agents, targeting a Factor XII (Hageman Factor (F12) gene, for treating subjects having a contact activation pathway-associated disease, such as a thrombophilia or hereditary angioedema (HAE), methods for preventing at least one symptom in a subject having a contact activation pathway-associated disease, such as a thrombus formation or an angioedema attack, and RNAi agents targeting an F12 gene, for use in the methods of the invention.
    Type: Application
    Filed: July 6, 2018
    Publication date: July 2, 2020
    Inventors: James Butler, Jingxuan Liu
  • Compositions and Methods for MYC Messenger RNA Inhibitors
    Publication number: 20200208151
    Abstract: The present invention provides compositions and methods of making and using novel MYC mRNA antisense inhibitors. In a particular embodiment, the invention features compositions and methods useful for the treatment of a condition (e.g., cancer).
    Type: Application
    Filed: August 31, 2018
    Publication date: July 2, 2020
    Inventors: Eric WiCKSTROM, Yuan-Yuan JIN
  • STABILIZED SARNA COMPOSITIONS AND METHODS OF USE
    Publication number: 20200208152
    Abstract: The invention relates to modified oligonucleotides, e.g., saRNAs useful in upregulating the expression of a target gene and therapeutic compositions comprising such oligonucleotides. Methods of using the oligonucleotides and the therapeutic compositions are also provided.
    Type: Application
    Filed: September 7, 2018
    Publication date: July 2, 2020
    Inventors: Hans E. Huber, David Blakey, Jon Voutila, Vikash Reebye
  • LONG NONCODING RNAS IN PULMONARY AIRWAY INFLAMMATION
    Publication number: 20200208153
    Abstract: The subject invention provides methods, assays, and products for preventing and/or treating pulmonary diseases. The present invention provides methods for identifying inhibitors of lncRNAs, pharmaceutical compositions comprising such inhibitors, and method of using said inhibitors in preventing and/or treating pulmonary airway inflammation. The present invention also develop novel strategies to modulate the mucous response for preventing, intervening, diagnosing and treating pulmonary airway inflammation, asthma, chronic obstructive pulmonary diseases (COPD) and Chronic mucus hypersecretion (CMH) pathogenesis.
    Type: Application
    Filed: October 30, 2019
    Publication date: July 2, 2020
    Applicant: THE FLORIDA INTERNATIONAL UNIVERSITY BOARD OF TRUSTEES
    Inventors: HITENDRA S. CHAND, MADHAVAN NAIR
  • ALLELE SELECTIVE INHIBITION OF MUTANT C9ORF72 FOCI EXPRESSION BY DUPLEX RNAS TARGETING THE EXPANDED HEXANUCLEOTIDE REPEAT
    Publication number: 20200208154
    Abstract: Provided herein are compositions and methods for reducing expression of C9orf72 transcripts in cells containing expanded intronic GGGGCC regions, including those in subjects having or at risk of developing amyotrophic lateral sclerosis (ALS) and frontotemporal dementia (FTD).
    Type: Application
    Filed: January 10, 2020
    Publication date: July 2, 2020
    Applicant: The Board of Regents of the University of Texas System
    Inventors: David COREY, Jiaxin HU
  • ENGINEERED NUCLEIC ACID-TARGETING NUCLEIC ACIDS
    Publication number: 20200208155
    Abstract: The present disclosure provides engineered Class 2 CRISPR-Cas-associated discontinuous first-stem nucleic-acid targeting nucleic acids, nucleoprotein complexes comprising these nucleic acids, and compositions thereof. Nucleic acid sequences encoding the Class 2 CRISPR-Cas-associated discontinuous first-stem nucleic-acid targeting nucleic acids, as well as expression cassettes, vectors and cells comprising such nucleic acid sequences, are described. Also, methods are disclosed for making and using the Class 2 CRISPR-Cas-associated discontinuous first-stem nucleic-acid targeting nucleic acids, nucleoprotein complexes comprising such nucleic acids, and compositions thereof.
    Type: Application
    Filed: March 11, 2020
    Publication date: July 2, 2020
    Inventors: Paul Daniel Donohoue, Andrew Paul May
  • APTAMER BIOSENSORS USEFUL FOR DETECTING HORMONES, HORMONE MIMICS, AND METABOLITES THEREOF
    Publication number: 20200208156
    Abstract: The present invention provides polynucleotides that bind to small molecules and methods of using the polynucleotides. In some embodiments, a method comprises detecting a small molecule with a polynucleotide. In other embodiments, a method comprises administering a polynucleotide to inhibit the action of a small molecule. In some embodiments, the small molecule is a hormone, a hormone mimic, phytoestrogen, opiate, opioid, endocrine disrupting compound, metabolite thereof, or variant thereof.
    Type: Application
    Filed: August 11, 2016
    Publication date: July 2, 2020
    Inventors: Shalen Kumar, Kenneth McNatty
  • RNA LIGAND-DISPLAYING EXOSOMES FOR SPECIFIC DELIVERY OF THERAPEUTICS TO CELL BY RNA NANOTECHNOLOGY
    Publication number: 20200208157
    Abstract: Disclosed herein are compositions comprising extracellular vesicles, such as exosomes, displaying an RNA nanoparticle on its surface. The RNA nanoparticle can target the extracellular vesicle to a given cell via a targeting moiety. The extracellular vesicle can also comprise a functional moiety, which can be used in treatment or diagnostics.
    Type: Application
    Filed: March 16, 2020
    Publication date: July 2, 2020
    Inventors: Peixuan Guo, Fengmei Pi, Hui Li, Shaoying Wang
  • PRODUCTS AND COMPOSITIONS
    Publication number: 20200208158
    Abstract: The present invention relates to products and compositions and their uses. In particular the invention relates to nucleic acid products that interfere with the TMPRSS6 gene expression or inhibits its expression and therapeutic uses such as for the treatment of hemochromatosis, porphyria and blood disorders such as ?-thalassemias, sickle cell disease and transfusional iron overload or myelodysplastic syndrome.
    Type: Application
    Filed: April 5, 2018
    Publication date: July 2, 2020
    Applicant: Silence Therapeutics GmbH
    Inventors: Sibylle DAMES, Ute SCHAEPER, Judith HAUPTMANN, Christian FRAUENDORF, Lucas BETHGE, Adrien WEINGÄRTNER
  • INHIBITION OF SEMA3A IN THE PREVENTION AND TREATMENT OF OCULAR HYPERPERMEABILITY
    Publication number: 20200208159
    Abstract: Described herein is a method of preventing or treating ocular vascular hyperpermeability including macular edema, in a subject comprising inhibiting Sema3A activity. Also disclosed are compositions and their use for preventing or treating Sema3A-dependent ocular vascular hyperpermeability.
    Type: Application
    Filed: March 4, 2020
    Publication date: July 2, 2020
    Inventor: PRZEMYSLAW SAPIEHA
  • MODULATION OF CARBON FLUX THROUGH THE MEG AND C3 PATHWAYS FOR THE IMPROVED PRODUCTION OF MONOETHYLENE GLYCOL AND C3 COMPOUNDS
    Publication number: 20200208160
    Abstract: The present disclosure provides methods of modulating the flux of carbon through the monoethylene glycol (MEG) biosynthesis pathway and one or more C3 compound biosynthesis pathways by expressing enzymes that are essential for improving C3 compounds and modulating other genetic aspects of MEG and C3 compound biosynthesis. The disclosure is further drawn to modified microbes comprising the disrupted sequences and overexpressed sequences, and compositions thereof.
    Type: Application
    Filed: December 27, 2019
    Publication date: July 2, 2020
    Inventors: Ane Fernanda Beraldi ZEIDLER, Beatriz Leite MAGALHAES, Lucas Pedersen PARIZZI, Veronica Maria Rodege Gogola KOLLING
  • NUCLEASE-MEDIATED DNA ASSEMBLY
    Publication number: 20200208161
    Abstract: Methods are provided herein for assembling at least two nucleic acids using a sequence specific nuclease agent (e.g., a gRNA-Cas complex) to create end sequences having complementarity and subsequently assembling the overlapping complementary sequences. The nuclease agent (e.g., a gRNA-Cas complex) can create double strand breaks in dsDNA in order to create overlapping end sequences or can create nicks on each strand to produce complementary overhanging end sequences. Assembly using the method described herein can assemble any nucleic acids having overlapping sequences or can use a joiner oligo to assemble sequences without complementary ends.
    Type: Application
    Filed: March 11, 2020
    Publication date: July 2, 2020
    Applicant: REGENERON PHARMACEUTICALS, INC.
    Inventors: Chris Schoenherr, John McWhirter, Corey Momont, Caitlin L. Goshert, Lynn Macdonald, Gregg S. Warshaw, Jose F. Rojas, Ka-Man Venus Lai, David M. Valenzuela, Andrew J. Murphy
  • NOVEL AGROBACTERIUM TUMEFACIENS STRAINS
    Publication number: 20200208162
    Abstract: The invention provides three novel disarmed strains of Agrobacterium tumefaciens bacteria useful for the transformation of plants. The invention provides three engineered A. tumefaciens Chry5 strains or bacterial cells thereof which comprise the Chry5 strain chromosomal background and a disarmed pTiChry5 vector, and methods of using said bacterial strains or cells for transformation of fungal or plant cells, in particular dicot or monocot plant cells, including soybean, maize, wheat, and sugarcane cells. The invention further relates to the transgenic plants created by these methods.
    Type: Application
    Filed: July 26, 2018
    Publication date: July 2, 2020
    Applicant: SYNGENTA PARTICIPATIONS AG
    Inventors: Zhongying Chen, Qiudeng Que, Mark Scott Rose, Heng Zhong, Mary-Dell Chilton, Eric Levy, Yingping Lucy Qin
  • METHODS FOR GENOMIC INTEGRATION IN PICHIA AND OTHER HOST CELLS
    Publication number: 20200208163
    Abstract: The present invention provides high efficiency targeted and marker-less single, double, triple, quadruple, and quintuple integrations by using CRISPR in host cells, including Pichia.
    Type: Application
    Filed: September 12, 2018
    Publication date: July 2, 2020
    Inventor: Hanxiao JIANG
  • PLANTS WITH SHORTENED TIME TO FLOWERING
    Publication number: 20200208164
    Abstract: There is described herein a mutant, non-naturally occurring or transgenic plant or part thereof having reduced expression of the gene encoding Terminal Flower 1 (TFL1) or reduced activity of the protein encoded by TFL1, said TFL1 comprising, consisting or consisting essentially of (i) a polynucleotide sequence comprising, consisting or consisting essentially of a sequence having at least 72% sequence identity to SEQ ID NO:7 or SEQ ID NO:8 or SEQ ID NO:10 or SEQ ID NO:11 or SEQ ID NO:19 or SEQ ID NO:20; or (ii) a polypeptide encoded by the polynucleotide set forth in (i); or (iii) a polypeptide having at least 72% sequence identity to SEQ ID NO:9 or SEQ ID NO:12 or SEQ ID NO:21; wherein the expression or activity of the polynucleotide or the polypeptide set forth in (i), (ii) or (iii) is reduced as compared to a control plant in which the expression or activity of the polynucleotide or the polypeptide set forth in (i), (ii) or (iii) has not been reduced.
    Type: Application
    Filed: December 15, 2017
    Publication date: July 2, 2020
    Inventors: Lucien Bovet, Simon Goepfert, Helene Laparra
  • Synthetic promoters useful for expression in plant cells
    Publication number: 20200208165
    Abstract: The present invention provides for a genetically modified plant cell or plant, comprising: (a) (i) one or more nucleic acids each encoding one or more transcription factors (or transcription activators) operatively linked to a first tissue-specific or inducible promoter, (ii) one or more nucleic acids each encoding one or more transcription repressors each operatively linked to a second tissue-specific or inducible promoter, or (iii) combinations thereof; and (b) one or more nucleic acids each encoding one or more independent genes of interest (GOI) each operatively linked to a promoter that is activated by the one or more transcription factors (or transcription activators), repressed by the one or more transcription repressors, or a combination of both.
    Type: Application
    Filed: March 11, 2020
    Publication date: July 2, 2020
    Applicant: The Regents of the University of California
    Inventors: Dominique Loque, Patrick M. Shih
  • Oleic Acid-Enriched Plant Body Having Genetically Modified FAD2 And Production Method Thereof
    Publication number: 20200208166
    Abstract: The present invention relates to an artificially manipulated unsaturated fatty acid biosynthesis-associated factor and use thereof to increase the content of a specific unsaturated fatty acid of a plant body. More particularly, the present invention relates to a system capable of artificially controlling unsaturated fatty acid biosynthesis and a plant body produced thereby, which include an artificially manipulated unsaturated fatty acid biosynthesis-associated factor to control unsaturated fatty acid biosynthesis and a composition capable of artificially manipulating the factor. In a specific aspect, the present invention relates to artificially manipulated unsaturated fatty acid biosynthesis-associated factors such as FAD2, FAD3, FADE, FAD7 and FAD8 and/or an unsaturated fatty acid biosynthesis controlling system by an expression product thereof.
    Type: Application
    Filed: September 26, 2017
    Publication date: July 2, 2020
    Inventors: Seok Joong KIM, Ok Jae KOO, Min Hee JUNG, Ye Seul KIM
  • Transgenic plants with enhanced agronomic traits
    Publication number: 20200208167
    Abstract: This invention provides recombinant DNA constructs, transgenic plant nuclei and cells with such recombinant DNA construct for expression of proteins that are useful for imparting enhanced agronomic trait(s) to transgenic crop plants. This invention also provides transgenic plants and progeny seed comprising the transgenic plant cells where the plants are selected for having an enhanced trait selected from the group of traits consisting of enhanced water use efficiency, enhanced cold tolerance, increased yield, enhanced nitrogen use efficiency, enhanced seed protein and enhanced seed oil. Also disclosed are methods for manufacturing transgenic seed and plants with enhanced traits.
    Type: Application
    Filed: January 9, 2020
    Publication date: July 2, 2020
    Inventors: Mark Scott Abad, Jeffrey E. Ahrens, Alice Clara Augustine, Erin Bell, Robert J. Bensen, Paolo Castiglioni, Richard Eric Cerny, Shobha Char, Xianfeng Chen, Jaishree M. Chittoor-Vijayanath, Maureen Daley, Jilll Deikman, Molian Deng, Todd DeZwaan, Stephen Duff, Michael D. Edgerton, Bradon J. Fabbri, Jason Fenner, Karen Gabbert, Barry S. Goldman, Deborah J. Hawkins, Steve He, Richard Johnson, Balasulojini Karunanandaa, Garrett J. Lee, Paul Loida, Savitha Madappa, Robert J. Meister, Donald E. Nelson, Anand Pandravada, Obed Patty, Ming Peng, Marie Petracek, Rajani Monnanda Somaiah, Dhanalakshmi Ramachandra, Monica P. Ravanello, Thomas G. Ruff, Rick A Sanders, Beth Savidge, Steven H. Schwartz, Padmini Sudarshana, Jindong Sun, Rebecca L. Thompson-Mize, Virginia Ursin, Dale L. Val, Srikanth Venkatachalayya, Tymagondlu V. Venkatesh, Kammaradi Vidya, K. Vijayalakshmi, Todd C. Weber, Jingrui Wu, Zhidong Xie, Wei Zheng
  • INCREASING PLANT GROWTH AND YIELD BY USING AN ABC TRANSPORTER SEQUENCE
    Publication number: 20200208168
    Abstract: Compositions and methods for improving plant growth are provided herein. Polynucleotides encoding ABC transporter proteins, polypeptides encompassing ABC transporter proteins, and expression constructs for expressing genes of interest whose expression may improve agronomic properties including but not limited to crop yield, biotic and abiotic stress tolerance, and early vigor, plants comprising the polynucleotides, polypeptides, and expression constructs, and methods of producing transgenic plants are also provided.
    Type: Application
    Filed: May 21, 2018
    Publication date: July 2, 2020
    Applicant: BENSON HILL, INC.
    Inventor: Matthew BEGEMANN
  • METHODS AND COMPOSITIONS FOR HERBICIDE TOLERANCE IN PLANTS
    Publication number: 20200208169
    Abstract: Provided herein are novel methods and compositions for conferring tolerance to auxin herbicides to plants. Also provided are herbicide tolerant plants, seeds, cells, and plant parts containing modified AUX/IAA proteins, as well as methods of producing the same.
    Type: Application
    Filed: April 13, 2018
    Publication date: July 2, 2020
    Inventor: Sherry L. LeClere
  • INSECTICIDAL PROTEINS AND METHODS FOR THEIR USE
    Publication number: 20200208170
    Abstract: Compositions and methods for controlling pests are provided. The methods involve transforming organisms with a nucleic acid sequence encoding an insecticidal protein. In particular, the nucleic acid sequences are useful for preparing plants and microorganisms that possess insecticidal activity. Thus, transformed bacteria, plants, plant cells, plant tissues and seeds are provided. Compositions are insecticidal nucleic acids and proteins of bacterial species. The sequences find use in the construction of expression vectors for subsequent transformation into organisms of interest including plants, as probes for the isolation of other homologous (or partially homologous) genes. The pesticidal proteins find use in controlling, inhibiting growth or killing Lepidopteran, Coleopteran, Dipteran, fungal, Hemipteran and nematode pest populations and for producing compositions with insecticidal activity.
    Type: Application
    Filed: March 5, 2020
    Publication date: July 2, 2020
    Applicants: PIONEER HI-BRED INTERNATIONAL, INC., E. I. DU PONT DE NEMOURS AND COMPANY
    Inventors: JENNIFER KARA BARRY, RYAN MICHAEL GERBER, LU LIU, AMY LUM, ERIC SCHEPERS, NASSER YALPANI, GENHAI ZHU
  • UNIVERSAL SELF-REGULATING MAMMALIAN CELL LINE PLATFORM FOR THE PRODUCTION OF BIOLOGICS
    Publication number: 20200208171
    Abstract: Disclosed are genetic control circuits, cells, and methods that use a repressor polypeptide to reduce the transcription rate of an exogenous therapeutic polypeptide encoding gene in response to a change in condition.
    Type: Application
    Filed: June 15, 2018
    Publication date: July 2, 2020
    Inventors: Robert YOUNG, Peter Michael O'CALLAGHAN, Thomas PAYNE
  • SCARLESS GENOME EDITING THROUGH TWO-STEP HOMOLOGY DIRECTED REPAIR
    Publication number: 20200208172
    Abstract: A method for scarless genome editing is disclosed. In particular, the method provides scarless genome modification by using homology directed repair (HDR) steps to genetically modify cells and remove unwanted sequences. This method can be used for genome editing, including introducing mutations, deletions, or insertions at any position in the genome without leaving silent mutations, selection marker sequences, or other additional undesired sequences in the genome.
    Type: Application
    Filed: January 10, 2020
    Publication date: July 2, 2020
    Applicant: The Board of Trustees of the Leland Stanford Junior University
    Inventors: Kazuya Ikeda, Matthew H. Porteus
  • ADENOVIRUS CONTROL VIRUS
    Publication number: 20200208173
    Abstract: Disclosed are compositions and methods related to replication deficient adenoviruses that are able to function as controls for nucleic acid diagnostic assays (e.g., nucleic acid sequencing based assays and/or nucleic acid amplification based assays).
    Type: Application
    Filed: June 17, 2016
    Publication date: July 2, 2020
    Inventors: Bharathi Anekella, Catherine Huang
  • SEQUENTIAL GENE EDITING IN PRIMARY IMMUNE CELLS
    Publication number: 20200208174
    Abstract: The invention pertains to the field of adaptive cell immunotherapy. It aims at reducing the occurrence of translocations and cell deaths when several specific endonuclease reagents are used altogether to genetically modify primary immune cells at different genetic loci. The method of the invention allows to yield safer immune primary cells harboring several genetic modifications, such as triple or quadruple gene inactivated cells, from populations or sub-populations of cells originating from a single donor or patient,for their subsequent use in therapeutic treatments.
    Type: Application
    Filed: June 30, 2017
    Publication date: July 2, 2020
    Inventors: Jean-Pierre CABANIOLS, Jean-Charles EPINAT, Philippe DUCHATEAU
  • METHOD, SYSTEM AND RECOMBINANT BACMID FOR PREPARATION OF RECOMBINANT ADENO-ASSOCIATED VIRUS
    Publication number: 20200208175
    Abstract: A method of preparing a recombinant adeno-associated virus (rAAV) including: (1) preparing a shuttle plasmid and a corresponding recombinant bacmid including a baculovirus genome, where the shuttle plasmid includes at least an rAAV gene of interest flanked by inverted terminal repeats (ITR-GOI) integrated with a heterologous functional gene fragment, and the recombinant bacmid includes an expression cassette of functional protein components necessary for assembly of the rAAV; (2) integrating the rAAV ITR-GOI and the expression cassette of functional protein components by using the shuttle plasmid and the recombinant bacmid, to yield a recombinant bacmid including a recombinant baculovirus genome; and (3) transfecting, with the recombinant bacmid, a host cell line.
    Type: Application
    Filed: June 28, 2019
    Publication date: July 2, 2020
    Inventors: Yang WU, Fuqiang XU, Ting MEI, Liangyu JIANG, Zengpeng HAN
  • METHOD OF INCREASING THE FUNCTION OF AN AAV VECTOR
    Publication number: 20200208176
    Abstract: A method of correcting singletons in a selected AAV sequence in order to increasing the packaging yield, transduction efficiency, and/or gene transfer efficiency of the selected AAV is provided. This method involves altering one or more singletons in the parental AAV capsid to conform the singleton to the amino acid in the corresponding position(s) of the aligned functional AAV capsid sequences.
    Type: Application
    Filed: March 13, 2020
    Publication date: July 2, 2020
    Inventors: Luk VANDENBERGHE, Guangping GAO, James M. WILSON
  • METHODS AND COMPOSITIONS FOR GENOME EDITING
    Publication number: 20200208177
    Abstract: Provided are methods and compositions for genome editing using sticky ends. Subject methods include (a) generating a staggered cut at each of two locations in genomic DNA of a target cell, thus generating two genomic staggered ends; and (b) providing/introducing a linear double stranded donor DNA that has staggered ends (i.e., sticky ends) that match/correspond to the sticky ends of the genomic DNA such that the sticky ends of the donor DNA hybridize with the sticky ends of the genomic DNA and the donor DNA is inserted into the genome. In some cases, the staggered cuts are generated by introducing into a target cell one or more sequence specific nucleases (or one or more nucleic acids encoding the one or more sequence specific nucleases).
    Type: Application
    Filed: April 17, 2019
    Publication date: July 2, 2020
    Inventors: Andre Ronald Watson, Christian Foster, Shuailiang Lin, Sara Marie Peyrot
  • PRODUCTION OF BETA-PHELLANDRENE USING GENETICALLY ENGINEERED PHOTOSYNTHETIC MICROORGANISMS
    Publication number: 20200208178
    Abstract: The present invention provides methods and compositions for producing ?-phellandrene hydrocarbons from a photosynthetic microorganism such as cyanobacteria.
    Type: Application
    Filed: February 18, 2020
    Publication date: July 2, 2020
    Inventors: Anastasios MELIS, Fiona K. Davies, Hsu-Ching Chen Wintz, Andreas Zurbriggen
  • SYSTEM AND METHOD OF BIOCATALYTIC CONVERSION FOR PRODUCTION OF ALCOHOLS, KETONES, AND ORGANIC ACIDS
    Publication number: 20200208179
    Abstract: Biocatalytic conversion systems and methods of producing and using same that have improved yields are disclosed. The systems and methods involve co-fermentation of sugars and gaseous substrates for alcohol, ketone, and/or organic acid production. The systems and methods may include biocatalytically converting at least one sugar substrate into at least one of alcohol, at least one ketone, and/or at least one organic acid. The systems and methods may further include biocatalytically converting gases that comprise CO2 and H2 to at least one alcohol and/or at least one organic acid, thereby adding extra revenue to biorefineries.
    Type: Application
    Filed: February 25, 2020
    Publication date: July 2, 2020
    Inventor: Hasan K. Atiyeh
  • PROCESSING BIOMASS TO OBTAIN HYDROXYLCARBOXYLIC ACIDS
    Publication number: 20200208180
    Abstract: Biomass (e.g., plant biomass, animal biomass, and municipal waste biomass) is processed to produce useful intermediates and products, such as hydroxy-carboxylic acids and hydroxy-carboxylic acid derivatives.
    Type: Application
    Filed: September 4, 2019
    Publication date: July 2, 2020
    Applicant: Xyleco, Inc.
    Inventors: Marshall Medoff, Thomas Craig Masterman, Andrew Papoulis, Jaewoong Moon, Jihan Khan, Robert Paradis
  • METHOD FOR PREPARING PHOSPHORYLATED KETO POLYOLS, DERIVATIVES THEREOF AND USES THEREOF
    Publication number: 20200208181
    Abstract: The present invention relates to a method for preparing phosphorylated keto polyols by biocatalysis and uses thereof.
    Type: Application
    Filed: May 22, 2018
    Publication date: July 2, 2020
    Inventors: VÉRONIQUE DE BERARDINIS, MARCEL SALANOUBAT, MARIELLE LEMAIRE, CHRISTINE GUERARD-HELAINE
  • NOVEL POLYPEPTIDE AND METHOD FOR PRODUCING ORNITHINE-BASED PRODUCT USING THE SAME
    Publication number: 20200208182
    Abstract: The present disclosure relates to a novel polypeptide having an ability to export an ornithine-based product, and a method for producing an ornithine-based product using the same.
    Type: Application
    Filed: June 14, 2018
    Publication date: July 2, 2020
    Inventors: Seon Hye KIM, Su Jin PARK, Kyoung Min LEE, Kyungsu NA, Hong Xian LI, Hyun-jung BAE, Jihyun SHIM, Young Lyeol YANG, Hye Won UM, Hyo Hyoung LEE, Min Gyeong KANG, Hye Won KIM, Byeong Cheol SONG, Haena OH, Han Hyoung LEE