Patents Issued in July 9, 2020
  • Publication number: 20200216839
    Abstract: Contiguity information is important to achieving high-quality de novo assembly of mammalian genomes and the haplotype-resolved resequencing of human genomes. The methods described herein pursue cost-effective, massively parallel capture of contiguity information at different scales.
    Type: Application
    Filed: October 28, 2019
    Publication date: July 9, 2020
    Applicant: University of Washington Through Its Center for Commercialization
    Inventors: Jay Ashok Shendure, Jerrod Joseph Schwartz, Andrew Colin Adey, Cho li Lee, Joseph Brian Hiatt, Jacob Otto Kitzman, Akash Kumar
  • Publication number: 20200216840
    Abstract: The present disclosure generally relates to sequencing two or more genes expressed in a single cell in a high-throughput manner using reverse transcriptases. More particularly, the present disclosure relates to a method for high-throughput sequencing of pairs of transcripts co-expressed in single cells (e.g., antibody VH and VL coding sequence) to determine pairs of polypeptide chains that comprise immune receptors.
    Type: Application
    Filed: July 27, 2018
    Publication date: July 9, 2020
    Inventors: Hidetaka TANNO, George GEORGIOU, Jonathan MCDANIEL, Gregory IPPOLITO, Andrew ELLINGTON
  • Publication number: 20200216841
    Abstract: Provided herein are compositions and methods to assess the genomic landscape of fixed cells using light activated oligonucleotides that can be directed to the nucleus, mitochondria, or cytoplasm of fixed cells and that, upon activation, can be extended for in situ copying of nuclear single-stranded DNA (i.e., open chromatin), open mitochondrial DNA, and/or cytoplasmic RNA into barcoded complementary DNA. These methods also provide for gene specific 3D chromatin structural niche analysis.
    Type: Application
    Filed: January 7, 2020
    Publication date: July 9, 2020
    Applicants: Agilent Technologies, Inc., The Trustees of the University of Pennsylvania
    Inventors: James EBERWINE, Jae-Hee LEE, Jifen LI, Stephen FISHER, Youtao LU, Junhyong KIM, Jai-Yoon SUL, Jinchun WANG, Mimi HEALY
  • Publication number: 20200216842
    Abstract: Disclosed herein are protein translation switches and conditional gene expression systems that are compatible with retroviral and lentiviral gene delivery. The linking of a protein translation switch to a 3? gene of interest suppresses translation of the gene of interest, and the alteration of the protein translation switch by DNA recombinase-mediated DNA recombination relieves the suppressed translation of the 3? gene of interest. Also disclosed herein are methods of mimicking clinical pharmacology in a pre-clinical setting.
    Type: Application
    Filed: October 11, 2018
    Publication date: July 9, 2020
    Applicant: Massachusetts Institute of Technology
    Inventor: Patrick J. Stern
  • Publication number: 20200216843
    Abstract: CRISPR/RNA-guided nuclease-related compositions and methods for treatment of A1AT deficiency and associated conditions are disclosed.
    Type: Application
    Filed: March 24, 2017
    Publication date: July 9, 2020
    Inventors: Shen SHEN, Penrose O'DONNELL, Minerva SANCHEZ
  • Publication number: 20200216844
    Abstract: The invention relates to iRNA, e.g., double-stranded ribonucleic acid (dsRNA), compositions targeting the Serpinc1 gene, and methods of using such iRNA, e.g., dsRNA, compositions to inhibit expression of Serpinc1 and methods of treating subjects having a bleeding disorder, such as a hemophilia.
    Type: Application
    Filed: December 14, 2018
    Publication date: July 9, 2020
    Inventors: Akin Akinc, Alfica Sehgal, Ivanka Toudjarska, Donald Foster, Stuart Milstein, Brian Bettencourt, Martin A. Maier, Klaus Charisse, Satyanarayana Kuchimanchi, Kallanthottathil G. Rajeev, Muthiah Manoharan
  • Publication number: 20200216845
    Abstract: The present invention relates to antisense oligonucleotides that are capable of modulating expression of RelA in a target cell. The oligonucleotides are complementary to mammalian RELA pre-m RNA intron sequence. The present invention further relates to conjugates of the oligonucleotide and pharmaceutical compositions and methods for treatment of cancer, inflammation or autoimmune diseases using the oligonucleotide.
    Type: Application
    Filed: January 10, 2018
    Publication date: July 9, 2020
    Inventors: Eva Marie W. LINDHOLM, Lykke PEDERSEN, Steffen SCHMIDT
  • Publication number: 20200216846
    Abstract: The present invention provides compounds comprising oligonucleotides complementary to a CLN3 transcript. Certain such compounds are useful for hybridizing to a CLN3 transcript, including but not limited to a CLN3 transcript in a cell. In certain embodiments, such hybridization results in modulation of splicing of the CLN3 transcript. In certain embodiments, such compounds are used to treat one or more symptoms associated with Batten Disease.
    Type: Application
    Filed: August 9, 2019
    Publication date: July 9, 2020
    Applicants: Ionis Pharmaceuticals, Inc., Rosalind Franklin University of Medicine and Science
    Inventors: Frank Rigo, Michelle L. Hastings
  • Publication number: 20200216847
    Abstract: The subject invention provides methods of treating neurological disease or disorder, such as brain injuries, such as stroke, traumatic brain injury (TBI), or other ischemic events that cause brain injury by inhibiting or down-regulating Let-7i activity or function. The disclosed methods may have the potential to extend the “window of opportunity” for treatment of such injuries and enhance the effectiveness of existing therapeutics.
    Type: Application
    Filed: August 13, 2018
    Publication date: July 9, 2020
    Inventors: MEHARVAN SINGH, CHANG SU, TRINH NGUYEN
  • Publication number: 20200216848
    Abstract: Provided herein are RNAi molecules for treating Huntington's disease. Further provided herein are expression cassettes, vectors (e.g., rAAV, recombinant adenoviral, recombinant lentiviral, and recombinant HSV vectors), cells, viral particles, and pharmaceutical compositions containing the RNAi. Yet further provided herein are methods and kits related to the use of the RNAi, for example, to treat Huntington's disease.
    Type: Application
    Filed: September 21, 2018
    Publication date: July 9, 2020
    Inventors: Catherine R. O'RIORDAN, Adam PALERMO, Brenda RICHARDS, Lisa M. STANEK
  • Publication number: 20200216849
    Abstract: The application discloses methods and compositions for the inhibition of the alternative complement pathway. The methods and compositions involve the use of aptamers for inhibiting complement Factor D. The application further provides anti-Factor D aptamers for the treatment of dry age-related macular degeneration, geographic atrophy, wet age-related macular degeneration or Stargardt disease. In some cases, stem-loop aptamers are provided for the inhibition of Factor D.
    Type: Application
    Filed: August 15, 2019
    Publication date: July 9, 2020
    Inventors: Carl Erickson, Christopher P. Rusconi, Kevin G. McLure, Matthew Levy, Arijit Bhowmick
  • Publication number: 20200216850
    Abstract: MicroRNAs embedded within an intron, which are called ‘mirtrons,’ can be used as a platform for expressing one or more shRNA or miRNA mimics in a lentiviral vector. The inventors developed a strategy to improve lentiviral titering by reducing the production of shRNA/miRNA from the vector during packaging through the introduction of splice-inhibiting antisense oligonucleotides during vector packaging, which inhibit the splicing of the mirtron and subsequent processing of the shRNAs/miRNAs. In an aspect is provided a kit comprising an oligonucleotide comprising a mirtron splice site binding sequence and a lentiviral packaging system. In an aspect is provided a method for producing a lentivirus. The method comprises the step of transfecting a cell with an oligonucleotide comprising a mirtron splice site binding sequence and a lentiviral packaging system; thereby producing the lentivirus.
    Type: Application
    Filed: September 21, 2018
    Publication date: July 9, 2020
    Inventors: John C. Burnett, Elizabeth Epps, John J. Rossi
  • Publication number: 20200216851
    Abstract: Disclosed are a recombinant poly(ethylene terephthalate) hydrolase (PETase) expression vector, a recombinant mono(2-hydroxyethyl)terephthalate hydrolase (MHETase) expression vector, a strain for producing each of the recombinant PETase and MHETase containing each of the vectors, and a method for degrading a plastics using each of the recombinant PETase and MHETase expressed therefrom. When the recombinant hydrolases, that is, PETase and MHETase are used together, high enzymatic activity may be sustained for a long time to completely degrade the PET.
    Type: Application
    Filed: December 2, 2019
    Publication date: July 9, 2020
    Applicant: Kyungpook National University Industry-Academic Cooperation Foundation
    Inventor: Kyung Jin KIM
  • Publication number: 20200216852
    Abstract: The present invention provides a tomaymycin biosynthetic gene cluster of Streptomyces species FH6421, and its use for producing 11-de-O-methyltomaymycm.
    Type: Application
    Filed: December 19, 2019
    Publication date: July 9, 2020
    Inventors: Claus LATTEMANN, Mark BROENSTRUP, Stefan WERNER, Rolf MÜLLER, Kirsten HARMROLS
  • Publication number: 20200216853
    Abstract: The invention relates to a plant genetic engineering field, and more particularly to a method for creating directed gene mutated non-transgenic plants. The method including performing a transgenic method onto directed gene mutated plants by introducing exogenous nucleic acid molecules; wherein the transgenic method includes introducing constructs into the directed gene mutated plants, each of the constructs contains a first nucleic acid molecule and a second nucleic acid molecule, wherein the first nucleic acid molecule serves as a gene editing element, and the second nucleic acid molecule serves as a lethal or stop development element, and can be used in a plant gene editing system such as CRISPR/CAS9. It can actively and automatically eliminate plant transgenic fragments, leaving enough time for gene editing elements to perform directed gene editing before removing transgenic fragments, providing a simple and effective method for gene editing without transgenic plants.
    Type: Application
    Filed: January 19, 2020
    Publication date: July 9, 2020
    Inventor: YUBING HE
  • Publication number: 20200216854
    Abstract: Isolated polynucleotides and polypeptides encoded thereby are described, together with the use of those products for making transgenic plants with increased tolerance to abiotic stress (e.g., high or low temperature, drought, flood).
    Type: Application
    Filed: February 18, 2020
    Publication date: July 9, 2020
    Inventors: Cory Christensen, Nestor Apuya, Kenneth A. Feldmann
  • Publication number: 20200216855
    Abstract: The present invention provide the use of Nicotiana benthamiana (N. benthamiana) HIR3s gene and/or Oryza sativa HIR3 gene in producing plants with resistance to virus and the method for making the plants thereof, the method involve: constructing NbHIR3.1, NbHIR3.2 or OsHIR3 into plant binary expression vector pCV1300 respectively, and introduced into Agrobacterium by electric shock, then transgenic plants overexpressing either NbHIR3.1 or NbHIR3.2 gene or tobacco or rice overexpressing HIR3 were produced by infection with Agrobacterium; the nucleotide sequences of NbHIR3.1, NbHIR3.2 and OsHIR3 are shown as SEQ ID NO:1, SEQ ID NO:2 and SEQ ID NO:23 respectively.
    Type: Application
    Filed: July 26, 2019
    Publication date: July 9, 2020
    Inventors: Fei YAN, Saisai LI, Yuwen LU, Jinping ZHAO, Jiejun PENG, Hongying ZHENG, Lin LIN, Ye CHENG, Jianping CHEN
  • Publication number: 20200216856
    Abstract: Disclosed is a dsRNA construct used to silencing specific eukaryotic translation initiation factor in plants to produce a plant resistant to viruses such as Potyviruses, Luteoviruses, and Furoviruses. More specifically, the plant would be resistant to viruses such as Wheat streak mosaic virus, Triticum mosaic virus, Soil bourne mosaic virus, or Barley yellow dwarf virus. Also disclosed are non-transgenic wheat plants having the genes for eIF(iso)4E-2 or eIF4G silenced.
    Type: Application
    Filed: March 19, 2020
    Publication date: July 9, 2020
    Inventors: John P. Fellers, Harold N. Trick, Luisa Cruz, Jessica Rupp
  • Publication number: 20200216857
    Abstract: The present disclosure provides materials and methods for treating a patient with one or more conditions or disorders associated with ATXN2 whether ex vivo or in vivo. For example, the present disclosure provides materials and methods for treating a patient with Spinocerebellar ataxia type 1 (SCA2). Also provided are materials and methods for editing a ATXN2 gene in a cell by genome editing. The present disclosure also provides materials and methods for altering the contiguous genomic sequence of a ATXN2 gene in a cell. In addition, the present disclosure provides one or more gRNAs for editing a ATXN2 gene. Also provided are therapeutics comprising at least one or more gRNAs for editing a ATXN2 gene. In addition, the present disclosure provides therapeutics for treating patients with a ATXN2 related condition or disorder.
    Type: Application
    Filed: February 21, 2018
    Publication date: July 9, 2020
    Applicant: CRISPR THERAPEUTICS AG
    Inventors: Ante Sven LUNDBERG, Samarth KULKARNI, Lawrence KLEIN, Hari Kumar PADMANABHAN
  • Publication number: 20200216858
    Abstract: The present invention provides novel donor polynucleotides formed by linking the two ends of a genomic fragment containing a cleavable site by a polynucleotide carrying a positive selection marker gene and a negative selection marker gene. Use of the donor polynucleotide makes it possible to modify only a target gene with avoiding the possibility of introducing mutations to sequences, called “off-target”, which are other than the target sequence, by introducing cleavage in a homologous site of the donor polynucleotide without introducing cleavage in a target gene locus.
    Type: Application
    Filed: July 19, 2018
    Publication date: July 9, 2020
    Applicant: I'ROM GROUP CO., LTD.
    Inventors: Kohji KUSANO, Takayuki KITOGO, Makoto INOUE, Tsugumine SHU, Toyotaka MORI
  • Publication number: 20200216859
    Abstract: An adenovirus comprising a sequence of formula (I) 5?ITR-B1-BA-B2-BX-BB-BY-B3-3?ITR wherein By comprises a transgene cassette containing four transgenes, said genes encoding a FAP-BITE, CXL10, CXL9, and IFN. The disclosure also extends to a pharmaceutical composition comprising the virus, and use of the virus or formulation in treatment.
    Type: Application
    Filed: August 28, 2018
    Publication date: July 9, 2020
    Inventors: Brian CHAMPION, Alice Claire, Noel BROMLEY
  • Publication number: 20200216860
    Abstract: The invention provides a recombinant RNA molecule comprising (i) a sequence of a gene-editing molecule mRNA, or a sequence of a functional fragment or derivative thereof, and (ii) at least one sequence of a coding or non-coding enrichment RNA, or a sequence of a functional fragment or derivative thereof, wherein the enrichment RNA, or functional fragment or derivative thereof, is capable of enhancing inclusion of the gene-editing molecule mRNA, or functional fragment or derivative thereof, into a retroviral particle. The invention provides a method of producing the retroviral particles of the invention, the method comprising culturing a packaging cell in conditions sufficient for the production of a plurality of retroviral particles.
    Type: Application
    Filed: September 5, 2018
    Publication date: July 9, 2020
    Applicant: Regeneron Pharmaceuticals, Inc.
    Inventors: Christos KYRATSOUS, Andrew J. MURPHY, Cheng WANG
  • Publication number: 20200216861
    Abstract: New gene therapy constructions and compositions are the subject of present invention. The gene therapy compositions consist in adeno-associated vectors which jointly express insulin (Ins) and glucokinase (Gck) genes. The new gene therapy constructions are useful for treatment of diabetes either in dosgs or human beings.
    Type: Application
    Filed: February 27, 2020
    Publication date: July 9, 2020
    Applicant: Universitat Autònoma de Barcelona
    Inventors: Fàtima Bosch Tubert, Eduard Ayuso López, Callejas Castiñeiras David
  • Publication number: 20200216862
    Abstract: Methods, systems, processes, and apparatuses are provided for delivery across cell membranes. In one aspect, an apparatus includes a substrate including a mixing channel, a process chamber, and a dilution channel to perform delivery of the payload to across the cell membranes. In another aspect, a system includes reservoirs for a cell suspension, a delivery solution, and a stop solution connected to a pump. The system further includes an agitator, a heater, a temperature controller, and a controller to operate the system. In yet another aspect, cells in suspension are mixed with a delivery solution in a microfluidic mixing chip. The delivery solution includes a permeabilization agent to cause permeabilization of the cells, allowing delivery of a payload from the delivery solution to the cells across the cell membranes.
    Type: Application
    Filed: July 18, 2018
    Publication date: July 9, 2020
    Inventor: Michael Maguire
  • Publication number: 20200216863
    Abstract: The present invention provides for a method to produce a biofuel and/or chemical compound from a biomass, the method comprising: (a) introducing a biomass and a deep eutectic solvent (DES), or mixture thereof, into a vessel to form a one-pot composition, wherein the DES, or mixture thereof, solubilizes the biomass; (b) introducing an enzyme and/or a microbe to the one-pot composition such that the enzyme and/or microbe produce a biofuel and/or chemical compound from the solubilized biomass; and, (c) optionally the biofuel and/or chemical compound is separated from the one-pot composition.
    Type: Application
    Filed: January 8, 2020
    Publication date: July 9, 2020
    Applicants: National Technology & Engineering Solutions of Sandia, LLC, The Regents of the University of California
    Inventors: Feng Xu, Blake A. Simmons, Seema Singh
  • Publication number: 20200216864
    Abstract: The present disclosure provides methods for utilizing genetically modified microbes to co-produce 3-hydroxypropionic acid (3-HP) and acetyl-CoA, and derivatives thereof from malonate semialdehyde as a common single intermediate. The disclosure further provides modified microbe that co-produce the 3-HP and acetyl-CoA derivatives from malonate semialdehyde.
    Type: Application
    Filed: December 18, 2019
    Publication date: July 9, 2020
    Inventors: Beatriz Leite MAGALHAES, Paulo Moises Raduan ALEXANDRINO, Felipe GALZERANI
  • Publication number: 20200216865
    Abstract: Certain embodiments provide a method for preparing a biochemical product (e.g., phenol, catechol, or muconic acid, or a salt thereof). For example, such methods include contacting a recombinant host having two or more recombinant pathways with a fermentable carbon source and growing the recombinant cell for a time sufficient to synthesize the product. In certain embodiments, each recombinant pathway: 1) is capable of producing the same final biochemical product; 2) comprises at least one gene encoding a polypeptide; 3) is derived from a different endogenous metabolite as its immediate precursor; and 4) converges to the same final product or the same intermediate metabolite.
    Type: Application
    Filed: July 16, 2018
    Publication date: July 9, 2020
    Applicant: ARIZONA BOARD OF REGENTS ON BEHALF OF ARIZONA STATE UNIVERSITY
    Inventors: David NIELSEN, Brian THOMPSON
  • Publication number: 20200216866
    Abstract: Provided is a method of producing a mixture of pure feedstock-based native polyphenols from a feedstock. Contaminant polyphenols are first removed from an enzyme solution for converting feedstock to a product to produce a polyphenol reduced enzyme solution. The polyphenol reduced enzyme solution is combined with the feedstock and the feedstock is converted to a product and by-product. Heretofore, there has been no process available to reduce or remove the contaminant phenols introduced to the feedstock by commercial enzyme solutions. This method allows for the removal of contaminant phenols prior to introduction to the processing stream and subsequent harvesting of pure feedstock 6 based native polyphenols. The pure feedstock-based polyphenols are removed from the product or by-product to produce a pure mixture of feedstock-based polyphenols.
    Type: Application
    Filed: July 26, 2018
    Publication date: July 9, 2020
    Inventor: Vincent Yacyshyn
  • Publication number: 20200216867
    Abstract: The invention provides a method of producing L-tryptophan, the method comprising culturing a L-tryptophan producing microorganism belonging to the Enterobacteriaceae family in a fermentation medium; wherein the L-tryptophan producing microorganism has been modified by enhancing the expression level of the mdfA gene or by enhancing the expression level of an mdfA allele.
    Type: Application
    Filed: January 5, 2020
    Publication date: July 9, 2020
    Inventors: Mechthild Rieping, Silke Jerrentrup, Nicole Dusch
  • Publication number: 20200216868
    Abstract: A two-step method for activating a cellulosic feedstock is described. The feedstock is subjected to a first high temperature activation step at a temperature greater than 190° C. and a second activation step at a lower temperature under alkali conditions. Also described are methods and compositions for the enzymatic hydrolysis of activated cellulose using one or more cellulase enzymes, a surfactant and polyaspartic acid. Also described are products of the methods.
    Type: Application
    Filed: March 20, 2020
    Publication date: July 9, 2020
    Applicant: Comet Biorefining Inc.
    Inventors: Andrew Richard, Dennis D'Agostino
  • Publication number: 20200216869
    Abstract: A method produces xylo-oligosaccharides from a biomass containing xylan and cellulose, which method is convenient and has a high yield of xylo-oligosaccharides because of inhibition of degradation of xylo-oligosaccharides into xylose. In the method of producing xylo-oligosaccharides, a biomass containing xylan and cellulose is hydrolyzed with a cellulase composition having at least activities of xylanase, cellobiohydrolase and ?-glucosidase and substantially free of ?-xylosidase activity during hydrolysis.
    Type: Application
    Filed: March 30, 2017
    Publication date: July 9, 2020
    Inventors: Takuya Kasahara, Chiaki Yamada, Hiroyuki Kurihara, Katsushige Yamada
  • Publication number: 20200216870
    Abstract: The invention relates to a method and device for amplifying non-purified nucleic acid, where the method comprises the following steps: Providing a reaction device in which an amplification reaction reagent is placed; Sampling with a sampler: the sampler includes a sealing block and a sample needle connected with the sealing block. The end of the sample needle is provided with a hydrophilic surface, which contacts the non-purified nucleic acid sample for sampling; the hydrophilic surface is inserted into the amplification reaction reagent, and the reaction device is sealed by the sealing block; The temperature of the reaction device is controlled by the temperature control instrument to carry out the amplification reaction. The device of the invention is used for realizing the method. The invention can save the process of sample preparation and purification, directly amplify the non-purified sample, reduce the operation difficulty, save the time and reduce the cost.
    Type: Application
    Filed: September 14, 2018
    Publication date: July 9, 2020
    Inventors: Xing SU, Kaiyuan WU
  • Publication number: 20200216871
    Abstract: Provided in certain embodiments are new methods for forming azido modified biomolecule conjugates of reporter molecules, carrier molecules or solid support. In other embodiments are provided methods for enzymatically labeling a biomolecules with an azide group.
    Type: Application
    Filed: January 9, 2020
    Publication date: July 9, 2020
    Inventors: Brian AGNEW, Kyle GEE, Tamara NYBERG
  • Publication number: 20200216872
    Abstract: The present invention is directed to the cells, compositions and methods for the production of recombinant protein, wherein an f-met group on the 5?-terminus is enzymatically removed. In particular, the invention is directed to a production process for obtaining high levels of soluble recombinant CRM197 protein from E. coli. Cells preferably contain one or more mutations of disulfide reductase genes, so that disulfide reductase activity is reduced. The invention also relates to purification method for CRM197 as well as characterization of properly folded CRM197 protein.
    Type: Application
    Filed: March 16, 2020
    Publication date: July 9, 2020
    Applicant: Fina BioSolutions, LLC
    Inventors: Natalia Oganesyan, Andrew Lees
  • Publication number: 20200216873
    Abstract: An immobilized enzymatic reactor can include a wall defining a chamber having an inlet and an outlet; a solid stationary phase covalently linked to an enzyme and disposed within the chamber; and a pressure modulator in fluid communication with the chamber and adapted to support continuous flow of a liquid sample comprising a polymer analyte through the inlet, over the solid stationary phase, and out of the outlet under a pressure between about 2,500 and 35,000 psi. In one example, the solid stationary phase includes inorganic/organic hybrid particles in an ultra performance liquid chromatography system, the enzyme is a protease, and the polymer analyte is a polypeptide. The immobilized enzymatic reactor can prepare an analyte for applications such as for hydrogen deuterium exchange mass spectrometry.
    Type: Application
    Filed: December 16, 2019
    Publication date: July 9, 2020
    Applicant: Waters Technologies Corporation
    Inventors: JOOMI AHN, MOON CHUL JUNG, KEVIN D. WYNDHAM
  • Publication number: 20200216874
    Abstract: Provided are a PCR primer pair and an application thereof. The PCR primer pair comprises a first primer and a second primer, wherein the first primer comprises a first specific sequence, a first random sequence, and a first universal sequence, the first specific sequence is located at the 3? end of the first primer, the first random sequence is located at the 5? end of the first primer, and the first universal sequence is located between the first specific sequence and the first random sequence; the second primer comprises a second specific sequence, a second random sequence, and a second universal sequence, the second specific sequence is located at the 3? end of the second primer, the second random sequence is located at the 5? end of the second primer, and the second universal sequence is located between the second specific sequence and the second random sequence, wherein the first random sequence and the second random sequence are inversely complementary.
    Type: Application
    Filed: June 20, 2017
    Publication date: July 9, 2020
    Inventors: Lin Yang, Haojun Jiang, Peng Zeng, Xuehan Zhuang, Ya Gao, Yanyan Zhang, Hui Jiang, Jing Guo, Fang Chen, Xun Xu
  • Publication number: 20200216875
    Abstract: The invention relates to a nucleic acid determination method, which is implemented by the sealed reaction vessel including a tubular chamber and a channel connecting the tubular chamber; the first tubular chamber is provided with the sample and the first set reaction reagent, and the nth tubular chamber is provided with the nth set reaction reagent; the method comprises the following steps: sealing the reaction vessel, conducting the first set of reactions in the first tubular chamber, and the products in the first tubular chamber are transported to the latter tubular chamber through a channel; after the product of the former tubular chamber is transported to the nth tubular chamber, the nth set reaction is carried out in the nth tubular chamber. The nucleic acid determination method provided by the invention can ensure the sealed closed system and convenient and simple operation.
    Type: Application
    Filed: September 14, 2018
    Publication date: July 9, 2020
    Inventors: Xing SU, Kaiyuan WU
  • Publication number: 20200216876
    Abstract: A disposable assay platform for detecting a target nucleic acid comprising multiple chambers and a method for operating the assay platform. Solutions containing the target nucleic acid move from one chamber to the next chamber by opening a vent pocket. The resulting pressure change enables the solution to flow to the next chamber. The platform comprises an electronic layer and one or more fluid layers bonded together. All heating operations can be performed by using resistive heating elements in the platform. All cooling operations are preferably passive. The platform is preferably operated when in a vertical orientation and can be docked to an external docking station that controls the operation of the platform.
    Type: Application
    Filed: November 8, 2019
    Publication date: July 9, 2020
    Inventors: Marc DEJOHN, Robert B. CARY, Nathan J. COBB
  • Publication number: 20200216877
    Abstract: The disclosure provides for methods, compositions, and kits for normalizing nucleic acid libraries, for example sequencing libraries.
    Type: Application
    Filed: February 4, 2020
    Publication date: July 9, 2020
    Inventors: Craig Betts, Glenn Fu
  • Publication number: 20200216878
    Abstract: The present invention relates to the field of RNA analysis. In particular, the invention concerns the use of a catalytic nucleic acid molecule for the analysis of an RNA molecule. The invention concerns methods for analyzing the 5? terminal structures of an RNA molecule having a cleavage site for a catalytic nucleic acid molecule.
    Type: Application
    Filed: March 4, 2020
    Publication date: July 9, 2020
    Applicant: CureVac Real Estate GmbH
    Inventor: Aniela WOCHNER
  • Publication number: 20200216879
    Abstract: This invention provides a DNA probe that is applicable to a DNA library prepared in a simple manner with excellent reproducibility. Such DNA probe is produced by a method comprising steps of performing a nucleic acid amplification reaction in a reaction solution containing genomic DNA and a random primer at a high concentration, so as to obtain a DNA fragments with the use of the genomic DNA as a template; determining the nucleotide sequence of the resulting DNA fragments; and, on the basis of the nucleotide sequence of the DNA fragments obtained in the step above, designing a DNA probe used for detecting a DNA fragment.
    Type: Application
    Filed: June 26, 2017
    Publication date: July 9, 2020
    Applicant: TOYOTA JIDOSHA KABUSHIKI KAISHA
    Inventors: Hiroyuki ENOKI, Yoshie TAKEUCHI, Minoru INAMORI
  • Publication number: 20200216880
    Abstract: Herein is reported a method for selecting a variant of a parental antibody variable domain encoding nucleic acid, wherein the parental antibody variable domain amino acid sequence encoded by said encoding nucleic acid has at least one developability hot spot, the method comprising the steps of (i) providing a multitude of DNA-containing samples (genomic material of antibody secreting B-cell) each including one or more antibody variable domain encoding nucleic acids; (ii) performing PCR amplification of said antibody variable domain encoding nucleic acids of (i) using consensus sequence-specific primers to obtain amplification products (wherein said consensus sequence-specific primers bind to consensus sequences that are common to a plurality of genes within the genetic loci set, thereby generating a pool of amplification products); (iii) sequencing a plurality of said amplification products obtained in step (ii) in order to determine the relative proportion of each nucleotide at each position in a sequencing
    Type: Application
    Filed: September 5, 2019
    Publication date: July 9, 2020
    Applicant: Hoffmann-La Roche Inc.
    Inventors: Guy GEORGES, Stefan KLOSTERMANN, Alain TISSOT, Francesca ROS, Alexander BUJOTZEK, Clemens WRZODEK, Frederic SCHULTZ
  • Publication number: 20200216881
    Abstract: The present invention concerns compositions, kits and methods for genetic variation detection and classification. These methods combine the specificity of a ligation reaction with the single-molecule sensitivity of nanopore biosensors. This invention can achieve clinical detection of many diseases, including bacterial infections and cancer entities, by identification of specific genetic alterations.
    Type: Application
    Filed: June 14, 2018
    Publication date: July 9, 2020
    Inventors: Amit MELLER, Michelle PATKIN, Tal GILBOA, Yana BEN ZVI ROZEVSKY
  • Publication number: 20200216882
    Abstract: The present application relates to new coumarin compounds and their uses as fluorescent labels. The compounds may be used as fluorescent labels for nucleotides in nucleic acid sequencing applications.
    Type: Application
    Filed: January 9, 2020
    Publication date: July 9, 2020
    Inventor: Nikolai Nikolaevich Romanov
  • Publication number: 20200216883
    Abstract: A method of sequencing a nucleic acid comprising the steps of (1) generating a stream of single nucleoside triphosphates; (2) producing at least one substantially double-stranded primary oligonucleotide used probe by reacting with a corresponding primary probe comprising (a) a first single-stranded oligonucleotide including a restriction endonuclease nicking-site, a single nucleotide capture site, and oligonucleotide flanking regions.
    Type: Application
    Filed: September 6, 2017
    Publication date: July 9, 2020
    Applicant: Base4 Innovation Limited
    Inventors: Barnaby BALMFORTH, Cameron Alexander FRAYLING
  • Publication number: 20200216884
    Abstract: A portable diagnostic device has a lysate stage with a port for receiving a sample and containing magnetic beads with a probe, and an outlet port. A series of assay stages are linked with the lysate vessel, each with a reservoir linked by channels. The final stage has a sensor for detecting beads attached to analyte molecules which have been conveyed according to attachment to probes on beads. Larger transport beads cause reporter beads which are tethered by target NA and probes to be transported to the final sensor stage, where they are released and detected when the transport beads have been removed.
    Type: Application
    Filed: September 6, 2018
    Publication date: July 9, 2020
    Applicant: ALTRATECH LIMITED
    Inventors: Brian O'FARRELL, Cian Desmond O'SULLIVAN, John O'DRISCOLL, Timothy CUMMINS, Paul FREE, Moira MCCARTHY, John WALSHE
  • Publication number: 20200216885
    Abstract: Described herein are products and processes for nucleic acid quantification, which are in part useful for detecting and determining the nucleotide sequence of rare nucleic acids (i.e., low copy number nucleic acids) in a sample. Such products and processes are useful for reducing the dynamic range among different nucleic acid species.
    Type: Application
    Filed: December 4, 2019
    Publication date: July 9, 2020
    Applicant: Sequenom, Inc.
    Inventor: Charles R. Cantor
  • Publication number: 20200216886
    Abstract: The invention relates to control compositions for a quantitative polymerase chain reaction. More particularly, the invention relates to control compositions for a quantitative polymerase chain reaction having at least one barcode sequence fragment and at least a first and a second primer binding site fragment, and to methods of their use.
    Type: Application
    Filed: December 13, 2019
    Publication date: July 9, 2020
    Inventor: Rachel R. SPURBECK
  • Publication number: 20200216887
    Abstract: A method is provided for preparing nanopore sequencing complexes in membranes for sequencing of polymers, e.g., polynucleotides and polypeptides. The nanopore sequencing complex is formed by the sequential linking of an enzyme to a nanopore that is inserted in a membrane, and of a polymer to the enzyme. Alternatively, the nanopore sequencing complex is formed by linking a preformed enzyme-polymer complex to a nanopore that is inserted in a membrane. The enzyme polymer complex is interchangeable.
    Type: Application
    Filed: January 20, 2017
    Publication date: July 9, 2020
    Applicant: Genia Technologies, Inc.
    Inventors: Timothy Kellogg Craig, Christos Tzitzilonis, Alexander H. Yang, Liv E. Jensen, Marshall Porter, Charlotte Yang, Corissa Harris, Matt Dipetro
  • Publication number: 20200216888
    Abstract: The present invention relates to a method for increasing the efficiency of read data analysis by removing primer sequence information present in a read obtained through next-generation sequencing (NGS) and, more specifically, to a method for matching information of a read and a designed primer to various reference values in several steps so as to determine primer sequence information within a read, and then precisely removing only a primer sequence so as to increase the efficiency of read data analysis. The method for increasing the efficiency of read data analysis in a primer removal-based NGS, according to the present invention, has a rapid data analysis speed and can precisely remove only a primer sequence, thereby being useful for increasing the efficiency and accuracy of read data analysis.
    Type: Application
    Filed: August 9, 2018
    Publication date: July 9, 2020
    Inventors: Chang Seon LEE, Chang Bum HONG, Ensel OH, Kwang Joong KIM