Abstract: A composition including: (i) a plurality of first particles comprising: about 25% to about 94% by weight a water soluble first carrier; and a perfume; wherein each of the first particles has a mass from about 1 mg to about 1 g; (ii) a plurality of second particles having: about 25% to about 94% by weight a water soluble second carrier; about 5% to about 45% by weight a quaternary ammonium compound formed from a parent fatty acid compound having an Iodine Value from about 18 to about 60; and about 0.5% to about 10% by weight a cationic polymer; wherein each of the second particles has a mass from about 1 mg to about 1 g; wherein the first particles and the second particles are in a package.

Abstract: A composition including a plurality of particles, the plurality of particles including: about 25% to about 94% by weight a water soluble carrier; about 5% to about 45% by weight a quaternary ammonium compound; and about 0.5% to about 10% by weight a cationic polymer; wherein the plurality of particles comprises individual particles that comprise at least one of the quaternary ammonium compound and the cationic polymer; wherein the individual particles differ from one another in weight fraction of at least one of the quaternary ammonium compound and the cationic polymer; and wherein the individual particles each have a mass from about 1 mg to about 1 g.

Abstract: A fabric care composition, including a fabric softening agent including esterquat and a co-softening agent including polyquatemium-7.

Abstract: A phosphate-free automatic dishwashing cleaning composition including from about 10% to about 60% by weight thereof of a builder system consisting of: i) a complexing agent selected from the group consisting of methyl glycine diacetic acid, its salts and derivatives thereof, glutamic-N,N-diacetic acid, its salts and derivatives thereof, iminodisuccinic acid, its salts and derivatives thereof, carboxy methyl inulin, its salts and derivatives thereof; and mixtures thereof; and ii) an itaconic/sulfonic polymer wherein the builder system comprises more than 40% by weight thereof of the complexing agent.

Abstract: A composition comprising a plurality of particles, wherein said particles comprise: a) 10 to 60 w.t.% polyethylene glycol, wherein the polyethylene glycol has a weight average molecular weight from 4000 to 12000; b) 0.1 to 50 w.t. % polysaccharide; c) 0.1 to 50 w.t.% ethoxylated non-ionic surfactant having a general formula: R1O(R2O)xH R1=a saturated fatty alcohol or polypropylene glycol. R2=C2H4 or mixture of C2H4 and C3H6x=8 to 120 and having a melting point between 40 and 60° C.; and d) 0.1 to 30 w.t. % perfume materials.

Abstract: A detergent formulation comprises 0.5 to 10 weight-percent mixed-charge polymer and one to 10 weight-percent nonionic surfactant, where weight-percent is relative to total detergent formulation weight, and wherein the mixed-charge polymer comprises quaternary ammonium groups and carboxylate groups extending as pendant side groups from the polymer backbone.

Abstract: Disclosed herein is a laundry or cleaning composition comprising (i) from about 0.001 wt. % to about 50 wt. % of at least one cationic polymer selected from the group consisting of poly acrylamidopropyl trimethyl ammonium chloride Poly(APTAC), polydiallyl dimethyl ammonium chloride poly(DADMAC), copolymers of polyAPTAC, copolymers of polyDADMAC, terpolymers of polyAPTAC, and/or terpolymers of polyDADMAC; (ii) from about 0.01 wt.% to about 50 wt.% of at least one non-ionic surfactant; (iii) optionally, from about 0.001 wt.% to about 5 wt.% of at least one enzyme; and (iv) optionally, from about 0.01 wt.% to about 25 wt.% of at least one laundry or cleaning additive, wherein said composition is capable of exhibiting color wash fastness or color maintenance.

Abstract: A composition and a method for clearing tissue for subsequent three-dimensional analysis. The clearing composition comprises: (1) a homogenizing agent such as N-methylglucamine, urea, or ethylenediamine; (2) a cytoplasmic, water-soluble refractive index adjusting agent such as iohexol, sodium thiosulfate, or polyethylene glycol; and (3) a membrane, lipid-soluble RI adjusting agent such as 2,2?-thiodiethanol (TDE) or propylene glycol. The tissue clearing composition is particularly suitable for use with long-term fixed human tissues.

Abstract: The invention relates to a process for removing red oils deposits formed in an installation comprising the use of a cleaning agent comprising one or more fatty acid esters to dissolve the red oils deposit and to form a mixture comprising the cleaning agent and the dissolved red oils; and removing the mixture comprising the cleaning agent and the dissolved red oils.

Abstract: A blade rinse composition comprising about 0.0001 to 0.01 wt. % disodium tetraborate decahydrate, about 0.0001 to 0.01 wt. % cationic surfactant, up to 5 wt. % anhydrous alcohol, and deionized water is disclosed, together with methods of making and using the same. The composition may prevent oxidation and/or contamination of metal blades and premature dullness of the blades.

Abstract: The present invention discloses a three-phase heavy-duty laundry detergent wipe, comprising a substrate 1 which is solid at ambient temperature as a third phase and a dispersion applied to the substrate and consisting of a liquid washing detergent 5 as a first phase and a water insoluble functional additive 2 as a second phase; and a method for manufacturing same. In particular, the second phase, i.e., the additive 2, of the dispersion can be a zeolite. The substrate 1 can consist of fatty alcohols, viscose, polyethylene, polypropylene or polyester.

Abstract: A method of making a fermented beverage, in particular a cereal-based fermented beverage, the method comprising the steps of: —Preparing a wort and boiling said wort, the boiled wort comprising a liquid fraction and a solids fraction; —Separating the liquid fraction from the solids fraction; —Fermenting the liquid fraction; —Maturing the fermented liquid fraction to obtain the fermented beverage wherein hop bine leaves and/or hop bine stems are added to the wort and maintained in contact with the liquid fraction of the wort for a period of at least 20 minutes at a temperature of ranging between 70-100° C.

Abstract: An alcoholic beverage maturing device is provided including a container and an ultrasonic energy device. The container defines an interior volume for holding an alcoholic beverage and the ultrasonic energy device is configured for subjecting the alcohol beverage within the interior volume of the container to ultrasonic energy. The ultrasonic energy device defines a range of exposure of ultrasonic energy to the interior volume of the container that is greater than 180 degrees in order to efficiently subject the alcoholic beverage contained therein to ultrasonic energy.

Abstract: A container system for aging beverages in a barrel and making and using it are disclosed. The system includes a container body having a first portion and a second portion each having an inner surface. The first portion and the second portion are selectively connected to enclose the barrel and form a space between an outer surface of the barrel and the inner surfaces of the first and second portions of the container body. The container body has at least one opening for inputting into the space and withdrawing from the space a medium.

Abstract: The present invention relates to fluidic systems for use in providing biomarkers for human Intestine On-Chip. More specifically, in some embodiments, a microfluidic chip containing intestinal epithelial cells co-cultured with intestinal endothelial cells in the presence of stretch and flow are used for identifying differentially expressed genes as biomarkers, e.g. for specific types of drug testing for use in treating gastrointestinal disorders or diseases related to intestinal function.

Abstract: The present disclosure provides a method of producing uniformly sized organoids/multicellular spheroids using a microfluidic device having an array of microwells. The method involves several successive steps. First, a microfluidic device containing parallel rows of microwells that are connected with a supplying channel is filled with a wetting agent. The wetting agent is a liquid that is immiscible in water. For example, the wetting agent may be an organic liquid such as oil. In the next step, the agent in the supplying channel and the microwells is replaced with a suspension of cells in an aqueous solution that contains a precursor for a hydrogel. Next, the aqueous phase in the supplying channel is replaced with the agent, which leads to the formation of an array of droplets of cell suspension in the hydrogel precursor solution, which were compartmentalized in the wells. The droplets are then transformed into cell-laden hydrogels.

Abstract: To provide a cell treatment container, including: a first member having a flow path in which a cell suspension including a liquid and cells dispersed in the liquid flows through, the flow path formed on a surface of the first member; a second member arranged to face the surface of the first member; and a damming formed in one or both of the first member and the second member, in which the damming is provided with a protrusion part protruding from the first member into the flow path to form a gap for allowing the liquid in the cell suspension to pass through the gap and for damming up the cells in the cell suspension, and a pillar extending from the protrusion part at a first end and being joined to the second member at a second end.

Abstract: Systems, methods, and apparatuses of controlling fluid flow are disclosed. An apparatus includes a first microplate having a first open portion and defining one or more first wells therein, a second microplate having a second open portion and defining one or more second wells therein, and a pneumatic lid constructed of styrene ethylene butylene styrene (SEBS). The pneumatic lid extends over the first open portion and the second open portion and includes one or more microfluidic channels that fluidly couple the one or more first wells to the one or more second wells. The pneumatic lid provides an airtight seal over the first microplate and the second microplate.

Abstract: Described herein is functionalized glass allowing for robust attachment of extracellular matrix proteins (ECM) withstanding extended culturing periods. By first treating glass with a sulfur silane reagent, the treated glass can be activated via an amine-sulfur linker, after which ECM proteins are attached to the linker. The Inventors observed that this glass treatment combination (sulfur silane-linker-ECM) resisted degradation when compared to conventional surface coatings, such as poly-L-orthinine coated glass.

Abstract: Provided is a culture device, including: a culture tank (110) configured to store an object liquid that is a culture solution having suspended therein an object; a screen (210) made of a metal, the screen including a main body (212) and a plurality of through holes (slits (214)) passing through the main body (212) from a front surface (212a) thereof to a back surface (212b) thereof; a spray portion (220) configured to spray the object liquid stored in the culture tank onto the front surface of the main body (212); an accommodation portion (240) surrounding the back surface (212b) of the main body (212) and configured to accommodate the culture solution having passed through the through holes; a UV light irradiation portion (250) arranged in the accommodation portion (240) and configured to radiate UV light; and a return portion (290) configured to return the culture solution in the accommodation portion (240) to the culture tank.

Abstract: The frequency at which a culture medium is replaced is reduced by prolonging the service life of the culture medium, the size of a device is reduced, cost is reduced, and a mechanical strength is enhanced. Provided is a cell culture device including: a culture container capable of accommodating cells and a culture solution; and an adsorbing member that is formed of a porous material having adsorption holes, the hole diameters of which are smaller than the cells and larger than impurities in the culture solution, and that adsorbs the impurities, without adsorbing the cells, in the culture solution by means of the adsorption holes while at least a portion of the adsorbing member is immersed in the culture solution in the culture container.

Abstract: The invention relates to an apparatus for the culture of cells, comprising a mixing apparatus and a reaction space which is bounded by a reactor wall, wherein the reactor wall has at least one connection for the introduction of gas and/or liquid and the reaction space has an internal volume, wherein the reaction space is configured so that the internal volume can be increased by at least 500%, based on a minimum internal initial volume, or that a horizontal cross-sectional area of the reaction space increases from the bottom upward. The invention further relates to a process for the culture of cells.

Abstract: Aspects of the present disclosure relate to systems and methods for manufacturing biologically-produced pharmaceutical products. Some of the systems described herein comprise an upstream component comprising a bioreactor and at least one filter (e.g., a filter probe) integrated with a downstream component comprising a purification module comprising at least a first partitioning unit and a second partitioning unit. In some embodiments, these integrated biomanufacturing systems may be operated under continuous or conditions and may be capable of efficiently producing pure, high-quality pharmaceutical products.

Abstract: An electroporation system may include a well plate, a dispenser and a dispenser-well positioning system. The well plate may include wells, each of the wells including an interior, a first electrode adjacent the interior and a second electrode adjacent the interior and spaced from the first electrode. The first electrode and the second electrode are to apply an electrostatic field across the well. The dispenser is to dispense a cell having a diameter into each of the wells. The dispenser-well positioning system is to align each well and the dispenser such that the dispenser dispenses the cell into each well at a location spaced from the first electrode and the second electrode by a distance of at least 5 times the diameter of the cell.

Abstract: The present disclosure relates to methods for control of cell growth rates where cell growth is measured in situ. The methods are applicable to bacterial cells, mammalian cells, non-mammalian eukaryotic cells, plant cells, yeast cells, fungi, and archea.

Abstract: Provided herein are systems comprising multiple fluidically-coupled chamber volumes. In particular, provided herein are cell culture systems that incorporate fluid flow between multiple cell culture volumes, and method of use thereof for the culture of multiple related cell/tissue types.

Abstract: An integrated process for the cultivation of algae or plants and the contemporaneous production of electric energy using a system in which a luminescent solar concentrator having a photovoltaic cell positioned on an outer side thereof is interposed between a cultivation area and a radiation source, totally or partially covering the cultivation area. The electric energy recovered from the photovoltaic cell is used in the cultivation of the algae or plants.

Abstract: The subject invention provides method of producing Trichoderma fungi on an industrial scale. In specific embodiments, the subject invention provides methods of producing both a liquid Trichoderma-based product and a solid-state Trichoderma-based product from the same starting seed culture and inoculant.

Abstract: Metabolites produced by a microorganism using oxaloacetate, pyruvate and/or acetyl-CoA as substrate or co-substrate upstream in the biosynthesis pathway, and more particularly using oxaloacetate. There is indeed a need in the art for transformed, in particular recombinant, microorganisms having at least an increased ability to produce oxaloacetate, pyruvate and/or acetyl-CoA, and in particular oxaloacetate, thus allowing an increased capacity to produce metabolites produced using oxaloacetate, pyruvate and/or acetyl-CoA as substrate or co-substrate upstream in the biosynthesis pathway, and in particular amino acids and their derivatives thereof, fatty acids, derivatives from the mevalonate pathway (in particular farnesyl, squalene, lanosterol, cholesterol and derivatives, and dolichols), flavonoides and/or polyketides. The solution proposed is the use of a genetically modified yeast comprising many modifications as described in the present text.

Abstract: The present invention relates to microbial cells, including but not limited to aerobic bacteria cells and anaerobic bacteria cells, as well as yeast cells, and methods for producing the cells, feed additives and compositions comprising the cells, and uses involving administration of the cells to animals.

Abstract: Provided herein are compositions and methods for expression of functional bacterial type I fatty acid synthase in photosynthetic hosts for production of activated acyl-CoAs and for use in biofuel production.

Abstract: The present disclosure discloses a preparation method for hyperthermophilic aerobic fermentation inoculant prepared by using sewage sludge, the method includes the following steps: carrying out fermentation after the activation of hyperthermophilic aerobic bacteria, removing the supernatant from the fermentation products, and adding the protective agent and stirring until uniform, drying to obtain a product, pulverizing the product by a pulverizer, and sieving the product before sub-packing. The solution of the present disclosure has the following advantages.

Abstract: The present disclosure provides the use of a phosphatidylcholine compound as a component of a media composition. The resulting media composition can be used for the cryopreservation of eukaryotic cells. The cryopreserved eukaryotic cells can be thawed and recovered for inoculation and/or growth in a media to reproduce new cells. Provided herein are compositions and methods for the cryopreservation of eukaryotic cells in a media composition containing a phosphatidylcholine compound.

Abstract: A cell-support matrix having narrowly defined uniformly vertically and non-randomly organized porosity and pore density and a method for preparation thereof. The matrix suitable for preparation of cellular or acellular implants for growth and de novo formation of an articular hyaline-like cartilage. A gel-matrix composite system comprising collagen-based matrix having a narrowly defined porosity capable of inducing hyaline-like cartilage production from chondrocytes in vivo and in vitro.

Abstract: A method for isolating the blastoderm of a non-human vertebrate embryo by enzymatic removal of the yolk.

Abstract: Provided is an isolated population of human pluripotent stem cells comprising at least 50% human pluripotent stem cells characterized by an OCT4+/TRA1-60?/TRA1-81?/SSEA1+/SSEA4? expression signature, and novel methods of generating and maintaining same in a pluripotent, undifferentiated state a suspension culture devoid of cell clumps. Also provided are novel culture media, cell cultures and methods for culturing pluripotent stem cells in a suspension culture or a two-dimensional culture system while maintaining the cells in a proliferative, pluripotent and undifferentiated state. The novel culture media comprise interleukin 11 (IL11) and Ciliay Neurotrophic Factor (CNTF); bFGF at a concentration of at least 50 ng/ml and an IL6RIL6 chimera; or an animal contaminant-free serum replacement and an IL6RIL6 chimera. Also provided are methods for generating lineage-specific cells from the pluripotent stem cells.

Abstract: Provided is a method for culturing immature oocytes. The method can promote in vitro maturation of the immature oocytes, and specifically comprises using follicular cells and a culture medium for culturing same. The culture medium for culturing the follicular cells contains CNP or variants thereof or analogues thereof and an HDAC (histone deacetylase) inhibitor. Also provided are the in vitro maturation culture medium containing CNP or variants thereof or analogues thereof and the HDAC inhibitor, and related compositions thereof, and the use of the above medium, culture medium and compositions in the promotion of in vitro maturation of the immature oocytes.

Abstract: A unified cell differentiation protocol for obtaining photoreceptor cells, retinal pigment epithelium, and 3D retinal organoid from pluripotent stem cells is described. Also described are photoreceptor cells, retinal pigmented epithelium, and 3D retinal organoid obtained from pluripotent stem cells. Also described are a pharmaceutical composition and a medicament containing the photoreceptor cells, retinal pigment epithelium, and 3D retinal organoid as described.

Abstract: There is disclosed an improved, safer and commercially efficient process for developing genetically engineered cells. More specifically, there is disclosed a process comprises introducing a donor DNA construct, a guide RNA, and an RNA-guided nuclease with the host cells to be transfected; and introducing the three components into the host cell. There is further disclosed a donor DNA construct designed for inserting a CAR (chimeric antigen receptor) into a defined genomic site of a host cell. Further, the present disclosure provides a host cell transfected with a CAR that lacks viral vectors that can present a safety concern. The disclosure provides for more efficient and more cost-effective process for engineering T cells to express CAR constructs.

Abstract: Methods of expanding tumor infiltrating lymphocytes (TILs), including peripheral blood lymphocytes and marrow infiltrating lymphocytes, from blood and/or bone marrow of patients with hematological malignancies, such as liquid tumors, including lymphomas and leukemias, and uses of such expanded TILs in the treatment of diseases such as cancers and hematological malignancies are disclosed herein.

Abstract: The present invention discloses a novel switchable dual chimeric antigen receptor-T (sdCAR-T) cell and a construction method and use thereof, which fall within the field of cellular immunotherapy for tumors. The dual chimeric antigen receptor consists of a first chimeric antigen receptor for MSLN and a second chimeric antigen receptor for FITC. A dual-targeted functional T cells regulated by specific exogenous bifunctional molecules is constructed, and the exogenous molecules are used to preliminarily discuss the in vivo and in vitro activity of the dual chimeric antigen receptor-T cell. By means of in vitro and in vivo tests, it is confirmed that the activation mode of the constructed CAR-T cell is controlled by the combination of endogenous tumor antigens and exogenous bifunctional molecules, and this combined regulation mode can significantly improve the safe application of CAR-T cell immunotherapy.

Abstract: The invention pertains to the field of adaptive cell immunotherapy. It provides with the genetic insertion of exogenous coding sequence(s) that help the immune cells to direct their immune response against infected or malignant cells. These exogenous coding sequences are more particularly inserted under the transcriptional control of endogenous gene promoters that are sensitive to immune cells activation. Such method allows the production of safer immune primary cells of higher therapeutic potential.

Abstract: In some embodiments provided herein is a method of preparing cargo-loaded platelets, comprising: treating platelets with a cargo and with a loading buffer comprising a salt, a base, a loading agent, and optionally ethanol, to form the cargo-loaded platelets.

Abstract: Certain embodiments are directed to compositions and methods for non-cytotoxic hematopoietic stem cell transplantation.

Abstract: The present disclosure provides a method for preparing heterogeneous hematopoietic stem and progenitor cells using non-mobilized peripheral blood, which uses a capsule culture system to capture and proliferate rare hematopoietic stem and progenitor cells in non-mobilized peripheral blood, and prepares heterogeneous hematopoietic stem and progenitor cell clones. The present disclosure captures the rare heterogeneous stem cells in non-mobilized peripheral blood and morphologically verifies the presence of heterogeneous hematopoietic stem and progenitor cells in non-mobilized peripheral blood. The method of the present disclosure has the characteristics of hematopoietic reconstitution, drug development, transplantation and immunotherapy, gene editing of cell types, and the like. The method of the present disclosure provides a reliable cell source for patient-specific functional hematopoietic stem cells, and actively promotes the clinical application of non-mobilized hematopoietic stem and progenitor cells.

Abstract: The present invention comprises a culture medium composition for the proliferation and differentiation of mesenchymal stem cells into osteoblasts, the composition comprising a bone morphogenic protein (BMP) complex with TGF-f3, bFGF, VEGF, and IGF, bound to collagen type 1 dissolved into a mesenchymal stem cell culture medium. Methods of use of the composition and/or the BMP complex comprise: performing a diagnostic assay detecting a level of bone morphogenetic proteins in a patient serum level; determining if the BMP level is below a threshold level; and when the BMP level is below the threshold level, administering a composition comprising a BMP complex, with or without a pharmaceutical, such as a drug for treating and/or preventing osteoporosis. The present invention comprises performing an in vivo lavage and bone assay; and administering the BMP complex composition to a patient in need thereof.

Abstract: The methods and compositions as disclosed herein describe the making of mature stem cell-derived cardiomyocytes for applications such as disease modeling, cardiotoxicity screening, drug screening and identification, among other uses. The methods involve physical and biochemical cues that promote a transition of stem cell-derived cardiomyocytes from a fetal phenotype to a more mature phenotype that more closely resembles that of adult cardiomyocytes.

Abstract: A method for preparing mesenchymal stem cell-derived exosomes on the basis of drug pretreatment, the method for preparing mesenchymal stem cell-derived exosomes comprising: using a statin to pretreat mesenchymal stem cells, and culturing the treated mesenchymal stem cells to collect exosomes secreted thereby. Also provided is an application of a statin in preparing a preparation for promoting the anti-apoptotic abilities and/or homing abilities of mesenchymal stem cells; and further provided is an application of a statin in preparing a preparation for promoting mesenchymal stem cells to secrete exosomes having myocardial infarction microenvironment-improving effects and/or myocardial repair abilities.

Abstract: The present method relates to methods of expanding or increasing stem cell production obtained from donor samples. The methods preferably including the steps of harvesting cells from minimally manipulated tissue using multiply harvesting cycles to increase the number of obtained stem cells.

Abstract: Ex-vivo culture systems are provided. Accordingly there is provided a culture system comprising a culture medium and a precision-cut tissue slice placed on a tissue culture insert, wherein the precision-cut tissue slice is maintained in a highly oxygenated atmosphere containing at least 50% oxygen and wherein said culture is rotationally agitated facilitating intermittent submersion of the tissue slice in the culture medium. Also provided are methods of culturing a tissue and methods of using the culture system for selecting a drug for the treatment of a disease.