Patents Issued in September 15, 2020
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Patent number: 10774274Abstract: The present invention relates to a combined reactor settler with the reactor. More specifically, the present invention relates to a combined reactor settler and reactor in a caustic free kerosene sweetening reactor which reduces equipment costs, operating costs, and plot space requirements.Type: GrantFiled: April 27, 2018Date of Patent: September 15, 2020Assignee: UOP LLCInventors: Susanna Kiki Wong, Jonathan A. Tertel
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Patent number: 10774275Abstract: An apparatus and a method are used for investigating the naphtha reforming process in catalyst test devices with reactors arranged in parallel. The apparatus has a plurality of reactors arranged in parallel with reaction chambers (R1, R2, . . . ), a product fluid supply, a process control, and at least one analysis unit. Each individual reactor has an outlet line for the product fluid stream, wherein the analysis unit is operatively connected to each outlet line for the product fluid stream and the apparatus is functionally connected to the control of the apparatus. In carrying out the method, naphtha-containing reactant fluid streams are brought into contact with catalysts in the individual reactors and the product fluid streams are subsequently supplied to the online analysis unit from the respective outlet lines of the individual reactors and analyzed. Using the evaluation of the online analytical characterization data, the process parameters of the respective reactor unit are adapted.Type: GrantFiled: December 22, 2015Date of Patent: September 15, 2020Assignee: hte GmbH the high throughput experimentation companyInventors: Marius Kirchmann, Christoph Hauber
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Patent number: 10774276Abstract: A stream of cracked naphtha is fractionated into at least four specified fractions defined by their respective boiling point ranges. The lightest fraction, IBP to 50° C., is treated in a selective etherification or alkylation process to reduce its RVP value and increase its RON. The second fraction, 50° C. to 150° C., is selectively hydrogenated to treat and convert the diolefins present and the treated stream is sent directly to the gasoline blending pool since it has the desired RON and low sulfur content. The third, and optionally a fourth fraction, boiling in the range of 50° C. to 180° C., in an embodiment, are utilized for the production of aromatics and the raffinate stream, after aromatic extraction, is sent to the gasoline blending pool. A fraction of this latter stream can optionally be recycled for further cracking to produce additional aromatics and gasoline blending components. The heaviest fraction, 180° C.Type: GrantFiled: October 9, 2018Date of Patent: September 15, 2020Assignee: SAUDI ARABIAN OIL COMPANYInventor: Ali H. Alshareef
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Patent number: 10774277Abstract: A process to prepare paraffins and waxes is provided, the process comprising: subjecting a Fischer-Tropsch product stream comprising paraffins having from 10 to 300 carbon atoms to a hydrogenation step, thereby obtaining a hydrogenated Fischer-Tropsch product stream comprising 10 to 300 carbon atoms; separating the hydrogenated Fischer-Tropsch product stream, thereby obtaining at least a fraction comprising 10 to 17 carbon atoms and a fraction comprising 18 to 300 carbon atoms; separating the fraction comprising 18 to 300 carbon atoms, thereby obtaining one or more first light waxes having a congealing point in the range of 30 to 75° C. and a second heavy wax having a congealing point in the range of 75 to 120° C.; and hydrofinishing one or more wax fractions having a congealing point in the range of 30 to 75° C. thereby obtaining one or more hydrofinished wax fractions having a congealing point in the range of 30 to 75° C.Type: GrantFiled: December 29, 2015Date of Patent: September 15, 2020Assignee: SHELL OIL COMPANYInventors: Arend Hoek, Antonius Adrianus Maria Roovers, Kaushik Majumder, Hélène Toledano
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Patent number: 10774278Abstract: A CO shift catalyst according to the present invention reforms carbon monoxide (CO) in gas. The CO shift catalyst has one of molybdenum (Mo) or iron (Fe) as a main component and has an active ingredient having one of nickel (Ni) or ruthenium (Ru) as an accessory component and one or two or more kinds of oxides from among titanium (Ti), zirconium (Zr), and cerium (Ce) for supporting the active ingredient as a support. The temperature at the time of manufacturing and firing the catalyst is equal to or higher than 550° C.Type: GrantFiled: June 8, 2017Date of Patent: September 15, 2020Assignee: MITSUBISHI HEAVY INDUSTRIES ENGINEERING, LTD.Inventors: Masanao Yonemura, Toshinobu Yasutake, Akihiro Sawata, Yoshio Seiki, Yukio Tanaka, Koji Higashino, Hyota Abe, Kaori Yoshida
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Patent number: 10774279Abstract: Compositions corresponding to marine diesel fuels, fuel oils, jet fuels, and/or blending components thereof are provided that include at least a portion of a natural gas condensate fraction. Natural gas condensate fractions derived from a natural gas condensate with sufficiently low API gravity can provide a source of low sulfur, low pour point blend stock for formation of marine diesel and/or fuel oil fractions. Natural gas condensate fractions can provide these advantages and/or other advantages without requiring prior hydroprocessing and/or cracking.Type: GrantFiled: September 20, 2018Date of Patent: September 15, 2020Assignee: EXXONMOBIL RESEARCH AND ENGINEERING COMPANYInventors: Scott K. Berkhous, Sheryl B. Rubin-Pitel, Kenneth C. H. Kar, Erin R. Fruchey
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Patent number: 10774280Abstract: The disclosure provides dyes for marking hydrocarbon compositions. More particularly, the disclosure relates to non-mutagenic dyes for marking hydrocarbon compositions.Type: GrantFiled: July 14, 2017Date of Patent: September 15, 2020Assignee: United Color Manufacturing, Inc.Inventors: Haresh Doshi, Michael Friswell, Thomas E. Nowakowski
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Patent number: 10774281Abstract: Provided is a process for limiting or preventing the formation and/or agglomeration of gas hydrates, comprising combining (1) a production fluid comprising an aqueous phase and one or more gasses and (2) at least one compound represented by formula (I) or a salt thereof as defined herein.Type: GrantFiled: November 25, 2016Date of Patent: September 15, 2020Assignee: Arkema FranceInventors: Tong Eak Pou, Frédéric Moser, Gilles Barreto, Jean-Philippe Gillet
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Patent number: 10774282Abstract: Compounds having the formula (F-I) below are provided herein: wherein R1 and R2 at each occurrence are independently a C1-C5000 alkyl group; R3 at each occurrence is independently hydrogen or a C1-C5000 alkyl group; R4 is a C1-C50 alkyl group or an unsubstituted or substituted phenyl group; R5 at each occurrence is independently hydrogen or a C1-C30 alkyl group; n is 1, 2, 3, or 4; and m+n is 5. Processes for preparing compounds of formula (F-I) as well as base stock and lubricant compositions containing compounds of formula (F-I) are also provided.Type: GrantFiled: December 4, 2017Date of Patent: September 15, 2020Assignee: ExxonMobil Chemical Patents Inc.Inventors: Kyle G. Lewis, Beth A. Fitch, Wenning W. Han
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Patent number: 10774283Abstract: A fully-formulating lubricating composition comprising an oil of lubricating viscosity; an ionic tetrahedral borate compound including a tetrahedral borate anion having a boron atom with two bidentate di-oxo ligands both being a linear C18-tartrimide; a first dispersant comprising a conventional ammonium substituted polyisobutenyl succinimide compound having a polyisobutenyl number average molecular weight of 750 to 2,500; a second dispersant comprising an ammonium substituted polyisobutenyl succinimde compound having an N:CO ratio of 1.8 and a polyisobutylenyl number average molecular weight of 750 to 2,500, wherein one or more of the first dispersant and the second dispersant are in cationic form. The fully formulated lubricating composition including one or more performance additives.Type: GrantFiled: July 21, 2017Date of Patent: September 15, 2020Assignee: The Lubrizol CorporationInventors: James D. Burrington, Ewan E. Delbridge, Binbin Guo, Jason J. Hanthorn
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Patent number: 10774284Abstract: The present disclosure relates to a high performance calcium sulfonate greases with enhanced performance benefits and methods for making the same.Type: GrantFiled: January 16, 2018Date of Patent: September 15, 2020Assignee: Afton Chemical CorporationInventors: Anand Karve, Vishal Nandurkar
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Patent number: 10774285Abstract: The disclosed technology relates to an additive composition and lubricating grease composition containing a synergistic combination of ingredients for inhibiting rust, particularly rust on mechanical devices subject to contact with salt water.Type: GrantFiled: December 21, 2015Date of Patent: September 15, 2020Assignee: THE LUBRIZOL CORPORATIONInventor: Scott Capitosti
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Patent number: 10774286Abstract: The present disclosure provides a grease composition with improved water resistance and mechanical stability at high-temperatures, including: at least one base oil; a water insoluble thickener; and a low molecular weight thixotropic polyamide composition having molecular weight distribution characteristics meeting the requirement described by the relationship: ( A + D ) ( B + C ) ? 0.05 where A=the % mass of the polyamide composition with a molecular weight greater than 1700 AMU; B=the % mass of the polyamide composition with a molecular weight between 1100 AMU and 1300 AMU; C=the % mass of the polyamide composition with a molecular weight between 700 AMU and 1000 AMU; and D=the % mass of the polyamide composition with a molecular weight of 600 AMU or lower (determined by GPC). The grease composition of the present disclosure provides a less than 50 penetration point change as determined by ASTM-D7342, a less than 10 mg weight loss as determined by ASTM-D4170, or both.Type: GrantFiled: December 12, 2018Date of Patent: September 15, 2020Assignee: EXXONMOBIL RESEARCH AND ENGINEERING COMPANYInventors: Sarvesh K. Agrawal, James E. Spagnoli, Chengrong Wang
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Patent number: 10774287Abstract: Traction fluid additive combination that is usable with any traction fluids base stock is provided. To optimize the traction, low temperature viscosity and shear stability performance, a combination of a polyisobutene and a polymethacrylate may be added to a base oil to obtain a traction fluid with good low temperature viscosity without degrading the shear strength properties of the fluid. To minimize foaming of the traction fluid, a combination of one, two, or more anti-foaming agents may be added to the fluid in addition to a standard additive package.Type: GrantFiled: March 6, 2019Date of Patent: September 15, 2020Assignee: VALVOLINE LICENSING AND INTELLECTUAL PROPERTY LLCInventors: Gefei Wu, Ning Ren, Frances E. Lockwood
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Patent number: 10774288Abstract: Compositions which are fragrant and contain at least a member set culled from a library of compositions, each being comprised of sub-combinations of selected terpenes. Fragrances that mimic that of various states of organic and synthetic aromatics including products, processes and those from non-combusted plant products, among other things, uniquitous products, processes, medicinals, and related moieties leverage databases of all known terpene groupings are offered for consideration, and have been provided, according to the instant teachings.Type: GrantFiled: August 25, 2014Date of Patent: September 15, 2020Assignee: The Werc Shop, LLCInventors: Sytze Elzinga, Jeffrey C. Raber
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Patent number: 10774289Abstract: Fragrance compounds having a unique chemical structure are provided, including 2-methoxy-2-methylheptane and derivatives thereof. The fragrance compounds can have fruity, radish, and/or herbaceous odor notes with a strong top note. The fragrance compounds can be used alone or incorporated into a fragrance composition and/or consumer product to modify or enhance the odor of the fragrance composition and/or consumer product.Type: GrantFiled: June 8, 2017Date of Patent: September 15, 2020Assignee: TAKASAGO INTERNATIONAL CORPORATIONInventors: Louis J. Lombardo, Michael E. Lankin, Frank Derosa, Maureen Blandino
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Patent number: 10774290Abstract: A detergent composition in the form of an effervescent tablet having high dissolution rate and good stability, which is suitable for producing aqueous solutions of cleaning compositions for cleaning hard surfaces and clothes. The tablet includes an alkylsulfate-type anionic surfactant, an effervescent system, a combination of at least two disintegrating agents, and urea.Type: GrantFiled: March 9, 2016Date of Patent: September 15, 2020Inventor: Lorena Martí Coma
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Patent number: 10774291Abstract: An automatic dishwashing composition is provided including a dispersant polymer; a builder; and a surfactant, wherein the surfactant is a fatty alcohol alkoxylate of formula I: wherein R1 is a linear or branched, saturated C8-24 alkyl group; R2 is a linear saturated C2-8 alkyl group; m has an average value of 22 to 42; n has an average value of 4 to 12; wherein m+n is an average value of 26 to 54; wherein the fatty alcohol alkoxylate has an average ethyleneoxy unit concentration per molecule, X, of >45 wt %; and, wherein the fatty alcohol alkoxylate has a ratio, Z, equal to X divided by n, wherein the ratio, Z, is <9.5.Type: GrantFiled: June 5, 2017Date of Patent: September 15, 2020Assignees: Dow Global Technologies LLC, Rohm and Haas CompanyInventors: Sara B. Klamo, Edward D. Daugs, Scott Backer, Severine Ferrieux, Paul Mercando, Eric P. Wasserman
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Patent number: 10774292Abstract: The present disclosure relates to compositions and methods of using the compositions for treating a floor surface. The disclosed compositions clean the floor surface, repair damage, or maintain the original look of the floor. The disclosed compositions also do not provide a permanent finish on the floor, are temporary coatings, or do not significantly change the gloss of the floor after application.Type: GrantFiled: May 2, 2018Date of Patent: September 15, 2020Assignee: Ecolab USA Inc.Inventors: Brian Robert Leafblad, Grant Daniel Lindh, Avila M. Hoffman, Traci Lynn Gioino, Dale Curtis Larson
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Patent number: 10774293Abstract: The present invention relates to polypeptide variants and methods for obtaining variants. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.Type: GrantFiled: July 12, 2017Date of Patent: September 15, 2020Assignee: NOVOZYMES A/SInventors: Lars Beier, Lars Henrik Oestergaard, Annette Helle Johansen, Klaus Gori, Thomas Holberg Blicher, Gernot Abel, Steen Krogsgaard, Jens E. Nielsen, Lars Giger
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Patent number: 10774294Abstract: The present disclosure provides stable unit dose compositions with enhanced pack rigidity. Such unit dose compositions comprise a liquid composition having at least four non-aqueous solvents, a beneficial composition, and a water-soluble container formed from a water-soluble or water-dispersible film material.Type: GrantFiled: April 18, 2018Date of Patent: September 15, 2020Assignee: Henkel IP & Holding GmbHInventor: Daniel Thomas Piorkowski
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Patent number: 10774295Abstract: Ethanol-containing nanolipid particles are disclosed which can be used in food products, frozen desserts, or beverages. The nanolipidic vehicles in which ethanol-containing substances are encapsulated can be combined with food products, desserts or beverage ingredients, including foods that are subsequently frozen. The frozen food product, dessert or beverage can remain in a frozen state during consumption by an individual. A composition comprising ethanol-containing nanolipid particles, which can be used in food products, frozen desserts, or beverages is disclosed.Type: GrantFiled: January 24, 2019Date of Patent: September 15, 2020Assignee: NuVessl, Inc.Inventor: Michael W. Fountain
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Patent number: 10774296Abstract: Cell culture devices are described herein that have notched lids, notched lids with corresponding gripping pads included on their bases, or gripping tabs included on their bases to minimize or eliminate the need for a user to contact a lid when attempting to lift or move a lidded cell culture device. In addition, methods for using the cell culture devices are also described herein.Type: GrantFiled: March 31, 2017Date of Patent: September 15, 2020Assignee: Corning IncorporatedInventors: Thomas Landon Carter, Gregory Scott Jordan
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Patent number: 10774297Abstract: A control unit includes a first connector configured to connect a proportional pressure regulator to a positive pressure supply and a second connector configured to connect the proportional pressure regulator to a negative pressure supply. The control unit further includes at least one sensor configured to detect an amount of air flow (volume per unit of time), positive or negative, within an air flow line connected to an output of the proportional pressure regulator, and a third connector configured to connect the air flow line to an air side of a diaphragm. Additionally, the control unit includes a controller programmed to control at least an opening and closing function of the proportional pressure regulator to attain a desired amount of air flow (volume per unit of time), positive or negative, within the air flow line.Type: GrantFiled: February 8, 2018Date of Patent: September 15, 2020Assignee: REPLIGEN CORPORATIONInventor: Rudolf Pavlik
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Patent number: 10774298Abstract: Described herein are systems, methods and kits for culturing and applying fresh microbial inoculants in the field. The system, methods and kits are easy to use, reliable, sealed to prevent contamination, and can be stored on location, for use on demand by those unskilled in the microbiological arts. Particular embodiments described herein may be used to increase agricultural crop yields.Type: GrantFiled: December 10, 2014Date of Patent: September 15, 2020Assignees: 3BAR BIOLOGICS, INC., OHIO STATE INNOVATION FOUNDATIONInventors: Bruce H. Caldwell, Brian B. McSpadden Gardener
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Patent number: 10774299Abstract: Disclosed is a jacketed, tiered baffle, bioreactor tank comprising an outer cylindrical-shaped jacket and a cylindrical tank having an inner tank surface defining a chamber configured for supporting a flexible bag disposed within the chamber, and an outer tank surface having tiered baffles configured for routing a heat exchange fluid around the entirety of the outer tank surface, the cylindrical tank disposed axially within the outer cylindrical-shaped jacket. The outer cylindrical-shaped jacket is sealed to the cylindrical tank in a manner sufficient to prevent or minimize loss of the heat exchange fluid.Type: GrantFiled: November 8, 2016Date of Patent: September 15, 2020Assignee: GLOBAL LIFE SCIENCES SOLUTIONS USA LLCInventors: Parrish M. Galliher, Thomas Erdenberger, Colin R. Tuohey, Joseph D. Crowell, Richard L. Damren
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Patent number: 10774300Abstract: The present invention is directed to methods and kits for separating, accumulating, characterizing and/or identifying microorganisms known to be present or that may be present in a test sample. The method of the invention comprises optional lysing non-microorganism cells and/or particulates that may be present in a test sample, followed by a subsequent filtration step for isolation and/or accumulation of microorganisms. The kit of the present invention may comprise at least one filter membrane or an integrated filtration and sample transfer device for isolation and/or accumulation of microorganisms.Type: GrantFiled: July 20, 2012Date of Patent: September 15, 2020Assignee: bioMerieux, Inc.Inventors: Jones M. Hyman, John D. Walsh, Ronnie J. Robinson, Christopher Ronsick
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Patent number: 10774301Abstract: A syringe device for separating liquids of different densities is provided with a hollow syringe barrel, a perforated plunger seal with a seal hole, and a hollow plunging tube with a closed bottom with at least one tube hole. The perforated plunger seal has an outer perimeter that resides flush against an interior surface of the hollow syringe barrel. The tube hole is in operational relationship with the seal hole. Optionally, a relief hole is provided on a top portion of the hollow plunging tube to allow a user to create vacuum pressure as necessary.Type: GrantFiled: July 10, 2017Date of Patent: September 15, 2020Assignee: Board of Trustees of Southern Illinois UniversityInventors: Ashim Gupta, Michael W. Neumeister, Sohyung Cho
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Patent number: 10774302Abstract: A method for dissecting and collecting one or more cells from a tissue sample fixed to an inner surface of a microfluidic device is described. The tissue sample is in fluid communication with a channel having an inlet end and an outlet end defined by the microfluidic device. The method comprises flowing a first fluid through the channel with a fluid flow from the inlet end to the outlet end; powering a laser to direct laser energy into the channel to impinge upon the first fluid proximate a first region of the tissue sample and cause fluid cavitation to thereby ablate a first set of one or more cells from the tissue sample; and collecting the first set of one or more cells within a first sample container coupled to the outlet end.Type: GrantFiled: March 28, 2017Date of Patent: September 15, 2020Assignee: BIOSYNTAGMA, LLCInventors: David Wayne Richardson, Dmitry Derkach, Colleen Ziegler, Steve Pemberton
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Patent number: 10774303Abstract: This disclosure describes techniques for using a single feedstock of barley to produce a fermented product and a method for filtering a large-particles stream from a liquid stream containing small particles of a process stream using a series of mechanical separation devices to increase yield.Type: GrantFiled: June 20, 2016Date of Patent: September 15, 2020Assignee: ICM, Inc.Inventors: Kurt A. Dieker, Charles C. Gallop, Scott D. Kohl, Keith Tjaden, Justin McMillen
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System and method for production of on-demand proteins in a portable unit for point of care delivery
Patent number: 10774304Abstract: A portable and mobile bioprocessing system and method for protein manufacturing that is compact, integrated and suited for on-demand production of any type of proteins and for delivery of the produced proteins to patients or for assay purposes. The portable system and method can also be used for efficient on-demand production of any type of protein with point-of-care delivery.Type: GrantFiled: October 23, 2019Date of Patent: September 15, 2020Assignee: UNIVERSITY OF MARYLAND, BALTIMORE COUNTYInventors: Govind Rao, Yordan Kostov, Leah Tolosa, Xudong Ge, Douglas Frey, Shayan Gholipour Borhani, David Burgenson -
Patent number: 10774305Abstract: Aspects of the invention relate to compositions comprising one or more live bacterial strains for topical administration to the skin, wherein the one or more live bacterial strains are Propionibacterium acnes (P. acnes) bacterial strains, and methods for use.Type: GrantFiled: April 20, 2016Date of Patent: September 15, 2020Assignee: S-Biomedic NVInventors: Bernhard Pätzold, Marc Güell
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Patent number: 10774306Abstract: A lactic acid bacterium having increased immunopotentiating activity with being mixed with no ester compound of polyol and a fatty acid is provided. A method for producing a lactic acid bacterium whose immunopotentiating activity is increased without coexisting with ester compound of polyol and a fatty acid, comprising: contacting a lactic acid bacterial immunopotentiating activity-increasing composition comprising an ester compound of a polyol and a fatty acid as an active ingredient with a lactic acid bacterium having immunopotentiating activity and then washing the lactic acid bacterium to remove the lactic acid bacterial immunopotentiating activity-increasing composition.Type: GrantFiled: November 7, 2016Date of Patent: September 15, 2020Assignee: Kirin Holdings Kabushiki KaishaInventors: Yukiko Kato, Masaya Kanayama
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Patent number: 10774307Abstract: The present invention discloses a method of inducing and differentiating human skin-derived precursors into corneal endothelial-like cells. The present invention utilizes human skin-derived precursors to induce corneal endothelial-like cells that are theoretically close to normal human corneal endothelial cells successfully by co-culturing with B4G12 corneal endothelial cells. Furthermore, the obtained corneal endothelial-like cells are applied to a corneal endothelial decompensation animal model, and corneal endothelium of the animal is successfully repaired, which has an important clinical application prospect.Type: GrantFiled: September 29, 2017Date of Patent: September 15, 2020Inventor: Xinyi Wu
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Patent number: 10774308Abstract: The invention provides a method for influencing the stability of an antibody producing cell, comprising directly or indirectly influencing the amount of BCL6 and/or Blimp 1 expression product within said antibody producing cell. Stable antibody producing cells and cell lines are also provided, as well as methods for producing antibodies using such cells and/or cell lines.Type: GrantFiled: August 28, 2018Date of Patent: September 15, 2020Assignees: ACADEMISCH MEDISCH CENTRUM BIJ DE UNIVERSITEIT VAN AMSTERDAM, AIMM THERAPEUTICS B.V.Inventor: Hergen Spits
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Patent number: 10774309Abstract: The invention provides a chimeric receptor comprising NKG2D, DAP10 and CD3 zeta. Also disclosed is a composition comprising this chimeric receptor and methods for making and using it to enhance the cytotoxicity and antitumor capacity of NK cells. The invention also encompasses methods for use of NKG2D-DAP10-CD3 zeta polypeptides, vectors and cells in methods for treating cancer and other proliferative disorders, as well as infectious diseases.Type: GrantFiled: December 28, 2017Date of Patent: September 15, 2020Assignees: ST. JUDE CHILDREN'S RESEARCH HOSPITAL, INC., NATIONAL UNIVERSITY OF SINGAPOREInventors: Dario Campana, Yu-Hsiang Chang
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Patent number: 10774310Abstract: Provided herein are populations of modified NK-92 cells, compositions and kits comprising the cells, and methods of making and using the populations of cells.Type: GrantFiled: May 28, 2019Date of Patent: September 15, 2020Assignee: NantKwest, Inc.Inventors: Hans Klingemann, Laurent Boissel, Patrick Soon-Shiong
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Patent number: 10774311Abstract: The present invention provides, in certain aspects, a natural killer (NK) cell that expresses all or a functional portion of interleukin-15 (IL-15), and methods for producing such cells. The invention further provides methods of using a natural killer (NK) cell that expresses all or a functional portion of interleukin-15 (IL-15) to treat cancer in a subject or to enhance expansion and/or survival of NK cells.Type: GrantFiled: August 26, 2019Date of Patent: September 15, 2020Assignees: National University of Singapore, St. Jude Children's Research Hospital, Inc.Inventors: Dario Campana, David Shook, Masaru Imamura
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Patent number: 10774312Abstract: It is an object of the present invention to provide a method for producing frozen mesenchymal cells maintaining a viability and an adhesion rate similar to those of cells obtained through a step of culture, without undergoing a step of culturing cells before cryopreservation step. The method comprises dissociating cells by treating a tissue with proteolytic enzymes and cryopreserving the cells. The present invention provides a method for producing frozen mesenchymal cells maintaining a viability and an adhesion rate similar to those of culturing cells without a step of culturings cells before cryopreserving.Type: GrantFiled: May 2, 2016Date of Patent: September 15, 2020Assignee: ADVANCED CELL TECHNOLOGY AND ENGINEERING LTD.Inventors: Toshimitsu Higa, Kenji Otani, Koichi Otomo
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Patent number: 10774313Abstract: Described is a simple plasma-based mammalian expression system that uses the RNA dependent RNA polymerase (RDRP) enzyme systems' activity for expression of recombinant proteins or RNA from viral minigenomes and rescue of recombinant viruses from cDNAs encoding entire genome(s) of negative stranded RNA viruses for use in synthesizing recombinant viruses and developing vaccines. This system will be used for expression of recombinant proteins, therapeutic RNA molecules including anti-sense and/or selecting interfering RNA and Ribozymes. This system can also be used for gene therapy and producing recombinant viruses for production of new vaccines.Type: GrantFiled: August 5, 2016Date of Patent: September 15, 2020Inventor: Vishwas Joshi
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Patent number: 10774314Abstract: The present invention relates to the production of chemical species in bacterial host cells. Particularly, the present invention provides for the production of chemical species in Escherichia coli (E. coli) host cells that functionally express engineered P450 enzymes.Type: GrantFiled: August 19, 2016Date of Patent: September 15, 2020Assignee: MANUS BIO INC.Inventors: Jason Donald, Christopher Pirie, Liwei Li, Huey-Ming Mak, Srishti Tibrewala, Ajikumar Parayil Kumaran
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Patent number: 10774315Abstract: Disclosed herein are glucosyltransferases with modified amino acid sequences. Such engineered enzymes synthesize alpha-glucan products having increased molecular weight. Further disclosed are reactions and methods in which engineered glucosyltransferases are used to produce alpha-glucan.Type: GrantFiled: September 11, 2018Date of Patent: September 15, 2020Assignee: DUPONT INDUSTRIAL BIOSCIENCES USA, LLCInventors: Yougen Li, Ellen D. Semke, Mark S. Payne, Jared B. Parker, Susan Marie Hennessey, Slavko Kralj, Veli Alkan, Richard Bott
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Patent number: 10774316Abstract: The invention includes methods for identifying polymerases, such as modified terminal nucleotidyl transferases (TdT), that are capable of binding nucleotides comprising removable 3?-O-blocking moieties to a nucleic acid initiator, without the use of a template. The invention further includes the identified polymerases, and methods of using the polymerases for de novo synthesis of predetermined oligonucleotide sequences.Type: GrantFiled: October 19, 2018Date of Patent: September 15, 2020Assignee: Molecular Assemblies, Inc.Inventors: J. William Efcavitch, Julie L. Tubbs, Prem Sinha, Boguslaw Stec
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Patent number: 10774317Abstract: The disclosure provides engineered enzymes that are capable of mediating the conversion of acetoacetyl-CoA to acetoacetate that do not react with the same order of magnitude with acetyl-CoA as they do with acetoacetyl-CoA (e.g., the engineered enzymes have a specific acetoacetyl-CoA hydrolase activity at least 10× higher than its acetyl-CoA hydrolase activity). Additionally, the disclosure provides modified microorganisms that comprise the engineered enzymes disclosed herein and methods of using same.Type: GrantFiled: June 18, 2018Date of Patent: September 15, 2020Assignee: BRASKEM S.A.Inventors: Avram Michael Slovic, Iuri Estrada Gouvea, Daniel Johannes Koch, Felipe Galzerani
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Patent number: 10774318Abstract: An object is to identify endoglucanase and ?-glucosidase genes by isolating genomic DNA containing cellulase genes, which are classified into endoglucanases or ?-glucosidases, from Acremonium cellulolyticus, and sequencing the nucleotide sequences thereof. The inventors intensively compared the amino acid sequences of known endoglucanases and ?-glucosidases with each other to find conserved region of amino acid sequences in Acremonium cellulolyticus, and various primers were designed based on the information. PCR was carried out using the various primers thus designed and genomic DNA or cDNA as a template. As a result, gene fragments of endoglucanases and ?-glucosidases were obtained. Primers were designed based on the gene fragments, and PCR was carried out to amplify nine genes of endoglucanases and ?-glucosidases. The nucleotide sequences thereof were sequenced, and the present invention was completed.Type: GrantFiled: July 18, 2018Date of Patent: September 15, 2020Assignee: MEIJI SEIKA PHARMA CO., LTD.Inventor: Fumikazu Yokoyama
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Patent number: 10774319Abstract: The present invention claims a novel process for the production and purification of microbial collagenase (Microbial Collagenase EC 3.4.24.3) produced by the non-pathogenic aerobic bacterium Vibrio alginolyticus chemovar. iophagus (NCIMB Number: 11038, synonym LMG 3418, hereinafter called Vibrio alginolyticus), which said process provides high production levels of collagenase with a stable, reproducible, cheap fermentation process. The collagenase produced from Vibrio alginolyticus according to the process described herein also presents a specific activity superior to that of other microbial collagenases, is stable in aqueous solution, and can be frozen without significant damage.Type: GrantFiled: July 12, 2017Date of Patent: September 15, 2020Assignee: FIDIA FARMACEUTICI S.P.A.Inventors: Susanna Vaccaro, Michele Caputo, Christian Cuppari, Giovanni Gennari
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Patent number: 10774320Abstract: The present invention relates to methods and recombinant microorganisms useful for the production of glycolic acid and/or glyoxylic acid. The methods of the invention involve either only one step of fermentation of the modified microorganisms of the invention or one step of fermentation of the modified microorganisms of the invention and one step of either biological or chemical conversion of the glycolic acid or of the glyoxylic acid, the microorganism of the invention over expressing a phosphoketolase gene.Type: GrantFiled: January 26, 2018Date of Patent: September 15, 2020Assignee: METABOLIC EXPLORERInventors: Wanda Dischert, Gwenaëlle Corre, Philippe Soucaille
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Patent number: 10774321Abstract: A method for monomerization of MMP-7 aggregates is provided. A method for monomerization of MMP-7 aggregates which comprises treating MMP-7 aggregates with a buffer solution comprising a monovalent cation chloride (sodium chloride, potassium chloride, etc.) at a low concentration or with a buffer solution not comprising a monovalent cation chloride, a process for preparing MMP-7 which involves said method for monomerization, and a (pharmaceutical) composition comprising MMP-7 in the aforementioned buffer solution. In case that a (pharmaceutical) composition comprising MMP-7 at a low concentration is prepared, the aforementioned buffer solution comprising sugar alcohols or sugars is used.Type: GrantFiled: May 20, 2015Date of Patent: September 15, 2020Assignee: KM BIOLOGICS CO., LTD.Inventors: Hiroshi Nakatake, Masaki Hirashima, Hideki Takeo, Reiko Matsuyama, Wataru Morikawa
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Patent number: 10774322Abstract: Disclosed are methods for lysis of cells, such as bacteria present in microbiomes, that combine three lysis steps—(1) heat, (2) detergent and (3) base—into a single step and that can be completed in a short period of time, e.g., a few minutes. The methods combine a normally incompatible detergent and base lysis, allows for simplified removal of detergent after lysis, and importantly, yields improved quantities of genomic DNA (gDNA) from difficult to lyse bacteria.Type: GrantFiled: December 26, 2017Date of Patent: September 15, 2020Assignee: Shoreline Biome, LLCInventors: Mark Driscoll, Thomas Jarvie
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Patent number: 10774323Abstract: The present invention relates to methods for displaying cyclic peptides on the surface of bacteriophage particles and collections thereof.Type: GrantFiled: December 21, 2015Date of Patent: September 15, 2020Assignee: LANTHIOPEP B.V.Inventors: Johannes Herbert Urban, Markus Andreas Moosmeier, Tjibbe Bosma, Josef Prassler