Abstract: Methods useful in detecting viable microbes in an agricultural composition are provided. Certain embodiments relate to methods in which a sample of an agricultural composition is obtained, and an amount of at least one pre-rRNA from at least one microbe is detected. The detected pre-rRNA from an agricultural sample may be compared with the amount of pre-rRNA from a control sample to determine the presence of a viable microbe.

Abstract: A method for testing presence or absence of a Mycoplasma in a specimen is disclosed. The method includes: culturing a specimen; extracting a nucleic acid from the cultured specimen and performing amplification of the nucleic acid using a primer capable of amplifying a nucleic acid sequence derived from a Mycoplasma, in which the specimen is a biotechnology-related product or a part thereof.

Abstract: The present invention relates to a method for producing RNA. In particular, the present invention relates to a method for producing RNA, which is scalable and provides RNA at a high purity. The present invention provides a method for producing RNA under GMP and/or cGMP-compliant conditions. The invention further provides specific processes for use as a quality control in the manufacturing of a template DNA and/or in a method for producing RNA, in particular by in vitro transcription.

Abstract: Provided is a labeling method for nucleic acid including a reaction step for hybridizing a nucleic acid probe that has a nucleotide sequence complementary to that of a nucleic acid to be labeled and contains a reactive nucleobase derivative incorporated at a position complementary to that of a target nucleobase as a target of labeling in the nucleic acid to be labeled, to the nucleic acid to be labeled; a transferring step for transferring a transfer group contained in the reactive nucleobase derivative to the nucleotide residue containing the target nucleobase in the nucleic acid to be labeled; and a labeling step for labeling the transfer group transferred to the nucleotide residue with a radioactive material.

Abstract: The present invention relates to a method for detecting a polynucleotide at a single-molecule level using FRET-PAINT method. When using the method for detecting of the present invention, problems of non-specific binding and off-target binding can be solved, and polynucleotides that are only 1 bp mismatch can be also distinguished.

Abstract: Provided herein are methods of normalizing nucleic acid libraries. The method uses nucleic acid probes with nucleic acid sequences that are complementary to one or more of these adaptor sequences are added to the nucleic acids libraries. The probes can hybridize to the adaptor sequences in the single stranded nucleic acid molecules derived from the libraries to form hybridization complexes. The probes are conjugated to a first binding member, which can interact with a second binding member that is conjugated to solid supports. The solid supports can then be collected and the single stranded nucleic acid molecules can be recovered in a volume of elution buffer to reach a desired concentration. As compared to standard methods, the methods are more efficient and cost-effective.

Abstract: Provided herein are compositions and methods for the synthesis, amplification, and in vitro transcription of full-length cDNA, or cDNA fragments. Methods are provided for reverse transcription of RNA and amplification for in vitro transcription. Further provided are method for loading of dendritic cells with the RNA and homologous lysate for immune stimulation.

Abstract: Embodiments of the present disclosure relate to nucleotides labeled with photoswitchable compounds. Also provided herein are methods and kits of using these labeled nucleotides for sequencing applications.

Abstract: Recognized herein is the need for methods and processes for increasing the efficiency and accuracy of paired end sequencing.

Abstract: This disclosure provides systems and methods for molecular identification and polymer (e.g., nucleic acid) sequencing using nanopores. The polymer may be passed through or in proximity to the nanopore and various subunits of the polymer may affect the current flowing through the nanopore. The various subunits may be identified by measuring the current at a plurality of voltages applied across the nanopore and/or membrane. In some cases, the polymerization of tagged nucleotides presents tag molecules to the nanopore that can be identified by measuring the current at a plurality of voltages applied across the nanopore and/or membrane. Also provided herein are systems and methods for sequencing both the sense and anti-sense strand of a double stranded nucleic acid molecule with a nanopore and methods for using ribonucleic acid (RNA) speed bump molecules to slow the passage of a nucleic acid molecule through or in proximity to a nanopore.

Abstract: Disclosed herein are methods for single-cell sequencing. In some examples, the methods include enriching a sample comprising a plurality of cells for cells of interest to produce an enriched cell sample; isolating one or more cells of interest in the enriched cell sample; and obtaining sequence information of one or more polynucleotides from each of the one or more isolated cells. Obtaining sequence information may include generating a molecularly indexed polynucleotide library from the one or more isolated cells. Enriching the sample may include focusing cells of interest in the sample using acoustic focusing.

Abstract: The invention relates to a sample processing apparatus comprising a holder for a microtiter plate comprising a plurality of microwells, optical measurement unit for measuring optical responses of samples dosed to the microwells, and a computing unit configured to analyze the optical responses in order to detect dosing failures in said plurality of microwells, and if a dosing failure has been detected in one or more of the microwells, to communicate the existence of the dosing failure to a user of the apparatus through signaling means or to store data indicative of the dosing failure to data storage means for further use. In particular, the invention relates to detecting dosing failures in before, during and after a PCR process.

Abstract: Diagnostic methods for assessing risk of or presence of inflammatory bowel disease in a patient based on glycosyltransferase or histo-blood group antigen signatures or a combination thereof. Also disclosed herein are prognostic methods for monitoring inflammatory bowel disease progression in a patient.

Abstract: The present invention provides a new method for detecting or monitoring a liver disease in a subject that has no indication of any liver pathologies, by measuring the amount of concentration of albumin mRNA in an acellular blood sample from the subject, and then comparing the amount or concentration of albumin mRNA with a standard control.

Abstract: Provided herein are methods for diagnosing the COPD inflammatory phenotype of an individual, and methods for selecting individuals with COPD for treatment based on their COPD inflammatory phenotype. In particular embodiments the methods comprise determining the level of expression of one or more genes selected from CLC, CPA3, DNASE1L3, IL1B, ALPL and CXCR2, or determining the expression profile CLC, CPA3, DNASE1L3, IL1B, ALPL and CXCR2, in one or more biological samples from an individual, wherein the expression levels or expression profile are indicative of the COPD inflammatory phenotype.

Abstract: The present invention relates to methods for determining Alzheimer's Disease (AD) in a patient. Further, the present invention relates to uses of polynucleotides for detecting mi RNAs in a blood sample isolated from a patient for determining Alzheimer's Disease in the patient. Furthermore, the invention relates to kits for determining Alzheimer's Disease in a patient.

Abstract: Methods and compositions disclosed herein generally relate to methods of improving clinical and economic outcomes to address adverse effects related to anesthesia, analgesics, opioids, and inadequate pain relief. Embodiments of the invention relate to the association between genes, specific polymorphisms of genes, and non-genetic factors with inadequate pain relief and anesthesia-, analgesic, and/or opioid-related adverse effects. Embodiments of the invention can be used to determine and manage patient risk factors for development of adverse perioperative effects and can allow for personalized anesthesia and pain management for improvement of pain control and reduction of anesthesia-, analgesic-, and opioid-related adverse outcomes. These methods and compositions apply to non-surgical pain management with opioids.

Abstract: Disclosed herein are methods of detecting an altered gene expression levels in a subject suspected of having an autoimmune disorder. Further described herein are methods of treating an autoimmune disorder in a subject having an exhibiting an altered gene expression level.

Abstract: The present invention relates, in part, to methods for the stratification, prognosis, diagnosis and stratification of a cancer, such as an ovarian cancer or a breast cancer.

Abstract: There is described herein a method of detecting the presence of DNA from cancer cells in a subject comprising: providing a sample of cell-free DNA from a subject; subjecting the sample to library preparation to permit subsequent sequencing of the cell-free methylated DNA; adding a first amount of filler DNA to the sample, wherein at least a portion of the filler DNA is methylated, then optionally denaturing the sample; capturing cell-free methylated DNA using a binder selective for methylated polynucleotides; sequencing the captured cell-free methylated DNA; comparing the sequences of the captured cell-free methylated DNA to control cell-free methylated DNAs sequences from healthy and cancerous individuals and from individuals with distinct cancer types and subtypes; identifying the presence of DNA from cancer cells if there is a statistically significant similarity between one or more sequences of the captured cell-free methylated DNA and cell-free methylated DNAs sequences from cancerous individuals.

Abstract: This invention relates to a method for diagnosing and/or prognosticating HER2-dependent cancer in a subject, comprising a) measuring the amount of moesin in a sample from the subject; b) comparing the amount of moesin measured in step a) to a reference value; c) finding a deviation or no deviation of the amount of moesin measured in step a) from the reference value; and d) attributing said finding of deviation or no deviation to a particular diagnosis and/or prognosis of HER2-dependent cancer in the subject.

Abstract: The present invention relates to the identification of a set of prognostic and theranostic markers enabling to assess the tumor microenvironment (immunogenic or immunosuppressive) of solid tumors and predict the responsiveness or the non-responsiveness to immunotherapy of the affected patient.

Abstract: The invention relates to a method of pre-surgical risk stratification of a prostate cancer subject, comprising determining a gene expression profile for phosphodiesterase 4D variant 7 (PDE4D7) in a biological sample obtained from the subject, determining gene expression profile, and determining a pre-surgical prognostic risk score for the subject based on the expression based risk score and pre-surgical clinical variables of the subject. This may allow for an improved stratification of the subject in a pre-surgical setting that may result in better primary treatment decisions. For instance, the pre-surgical prognostic risk score may allow to make better recommendation on whether to select active surveillance vs. active intervention, e.g., radical prostatectomy, for certain sub-populations of prostate cancer patients.

Abstract: The present invention relates to methods and compositions for differentiating between absence of disease, endometriosis, and EAOC or serous ovarian cancer in a subject. It is based, at least in part, on the discovery that certain microRNAs are associated with each of these conditions.

Abstract: The invention provides methods, nucleic acids and kits for determining the prognosis of a subject having cancer. The invention discloses genomic sequences the methylation patterns of which have utility for the improved detection of said disorder, thereby enabling the improved diagnosis and treatment of patients.

Abstract: Disclosed herein, in certain embodiments, are methods of detecting the presence of a skin cancer based on molecular risk factors. In some instances, the skin cancer is cutaneous T cell lymphoma (CTCL). In some cases, the skin cancer is mycosis fungoides (MF) or Sézary syndrome (SS).

Abstract: An example method of treating a subject having a tumor is described herein. The method can include determining a radiosensitivity index of the tumor, deriving a subject-specific variable based on the radiosensitivity index, and obtaining a genomic adjusted radiation dose effect value for the tumor. The radiosensitivity index can be assigned from expression levels of signature genes of a cell of the tumor. Additionally, the genomic adjusted radiation dose effect value can be predictive of tumor recurrence in the subject after treatment. The method can also include determining a radiation dose based on the subject-specific variable and the genomic adjusted radiation dose effect value.

Abstract: This document provides methods and materials involved in using measles viruses. For example, methods and materials for identifying mammals (e.g., humans) likely to respond to standard measles virus vaccines or standard measles virus-based therapies as well as methods and materials for identifying mammals (e.g., humans) unlikely to respond to standard measles virus vaccines or standard measles virus-based therapies are provided.

Abstract: The present invention relates to polymorphisms indicative of altered milk fatty acid composition in female milk-producing cattle. In particular, the present invention provides methods for selecting a cattle which possesses a genotype which in female milk-producing cattle is indicative of a desired milk fatty acid composition and cattle selected by said method. Further, the present invention provides milk produced by the female milk-producing cattle, methods for selective breeding and non-human gametes. Use of a nucleic acid molecule or an oligonucleotide in an in vitro method for determining the presence of at least one allele, which in a female milk-producing cattle is indicative of a desired milk fatty acid composition, is also part of the present invention.

Abstract: The invention provides a lysis reagent for lysing red blood cells, thereby releasing a target, such as RNA from a parasitic organism, in a form suitable for analysis. The reagent includes at least ammonium chloride and an anionic detergent, and may include an anti-coagulant. The reagent serves to lyse red blood cells, protect the released target from degradation in the lysate, and is compatible with subsequent steps for analysis of the target such as target capture, amplification, detection, or sequencing.

Abstract: Provided is a Bifidobacterium animalis subsp. Lactis GFC-B09 strain and a cosmetic composition containing the same. The Bifidobacterium animalis subsp. Lactis GFC-B09 strain (Accession number: KCCM12263P) or a culture solution thereof has the ability to increase the amount of S. epidermidis, to degrade histamine, to inhibit allergies, to repair DNA, and to increase wrinkle resistance, skin whiteness, skin density, and skin elasticity, and thus can exhibit excellent effects as a cosmetic composition when contained as an active ingredient in a cosmetic base.

Abstract: An orifice-type quenching nozzle for an induction hardening system includes a body having a plurality of nozzle orifices configured to apply a quenching fluid onto a to-be-quenched workpiece. The nozzle orifices are arranged on at least one surface of the body in rows and in columns, and the plurality of nozzle orifices are positioned such that each nozzle orifice is located a same distance from each directly adjacent nozzle orifice.

Abstract: Provided are a high-strength galvannealed steel sheet, and a manufacturing method therefor. The manufacturing method is for a high-strength galvannealed steel sheet which includes a zinc coated layer with a coating weight of 20 to 120 g/m2 per side on a surface of a steel sheet having a chemical composition containing, in mass %, C: 0.03 to 0.35%, Si: 0.01 to 1.00%, Mn: 3.6 to 8.0%, Al: 0.001 to 1.00%, P: 0.100% or less, and S: 0.010% or less, with the balance being Fe and inevitable impurities. The method includes: setting a cold rolling reduction ratio to 20% or more and 35% or less when cold rolling is performed on the steel sheet, and setting a maximum steel sheet temperature to be reached in an annealing furnace to 600° C. or higher and 700° C. or lower when annealing is further performed on the steel sheet.

Abstract: Provided are a high-strength steel sheet having a yield strength of 550 MPa or more and excellent fatigue-strength of a weld and a method for manufacturing the steel sheet. A high-strength steel sheet has a specified chemical composition, a steel microstructure observed in a cross section in a thickness direction parallel to a rolling direction including 40% to 75% of a martensite phase in terms of volume fraction, in which a total volume fraction of martensite grains whose average grain diameter ratios with respect to adjacent ferrite grains are ¼ or more and 1 or less is 60% or more with respect to an entire martensite phase, and a yield strength (YP) of 550 MPa or more.

Abstract: A method for manufacturing a lightweight steel plate with ultrahigh strength and high toughness includes, in percent by weight: 0.30-0.45 wt % C, 1.0-2.0 wt % Si, 2.0-4.0 wt % Al, 6.0-7.0 wt % Mn, 0.30-0.50 wt % V, 0.02-0.05 wt % Nb, 0.001-0.005 wt % B, N?0.003 wt %, P?0.015 wt %, S?0.005 wt %, Fe, and inevitable impurities.

Abstract: A method for recovering valuable elements from pre-combustion coal-based materials includes the steps of grinding the materials to a predetermined size, roasting the ground materials at a temperature of 600° C.-700° C. for a predetermined residence time needed for mineral decomposition, submerging the roasted, ground materials in a solution of lixiviant, filtering the lixiviant solution to separate residual solids from a pregnant leach solution including the valuable elements and recovering and concentrating the valuable elements from the pregnant leach solution.

Abstract: Presented herein is a ligand-assisted elution chromatography process for the separation of metal ions using a sorbent. In particular, the present invention discloses a process of two sets of column system in combination with two sets of eluting ligand solutions to prepare substantially pure rare earth elements, wherein the first set of column comprises strong acid cation exchange resins and the second set of chromatographic columns comprises hydrous polyvalent metal oxide selected from the group consisting of TiO2, ZrO2, or SnO2 and wherein ligand of said second ligand solution coordinates with said hydrous polyvalent metal oxide.

Abstract: Provided is a method for easily and inexpensively separating a rare earth element contained in an aqueous solution.

Abstract: Recovery of rare earth elements (REEs) from electronic wastes is a promising approach. The existing methods for separation of REE from the secondary sources are not economically viable and scalable. A method and system for separation of rare earth metals from a plurality of secondary sources has been provided. The magnet is obtained from the secondary sources which is then crushed to a coarser size. The powder is then demagnetized by heating and roasted at high temperature to obtain the metal oxides. The metals oxides are then dissolved by acid leaching to obtain leach liquor. Iron is removed from leach liquor by precipitation and separated by filtration. The individual REE is then separated by liquid-liquid extraction. The conditions in liquid-liquid extraction are adjusted such that only desired REE is separated. The extracted REE is then stripped out by acid. The individual rare earth element is then precipitated and dried.

Abstract: Provided is a method of preparing an alloy powder, comprising the steps of: melting the metal elements to produce the alloy solution; atomizing the alloy solution into small drops under oxygen-containing atmosphere; forcing the small drops to be quickly cooled under the driving of the atomizing flow to obtain the alloy powder; wherein, when the method is used to prepare Cu—In—Ga alloy powder, Cu/(In+Ga) is 0.5 to 1.1, In/(In+Ga) is 0.2 to 0.9, Ga/(In+Ga) is 0.1 to 0.8, In/(In+Ga)+Ga/(In+Ga) is 1. Also provided is an alloy powder and a method of preparing Cu—In—Ga alloy powder.

Abstract: An improved sterling silver alloy. Like all sterlings, the improved alloy is at least 92.5 percent silver by weight. It has less copper than traditional sterlings: 3.0 percent versus the traditional 7.5 percent. Additionally, the improved alloy includes about 2.75 percent palladium, about 1.0 percent tin, and about 0.75 percent zinc, all by weight. A grain refiner, such as ruthenium, may also be provided. The components of the preferred alloy are melted, degassed, remelted, and then formed into casting grains, wire, and etc. The resulting alloy is significantly harder, as cast, than traditional sterlings: 95-120 Vickers versus 65 Vickers for traditional sterlings. The improved alloy also exhibits improved corrosion resistance. Other than a slightly higher (<200° F.) liquidus temperature, the improved alloy may be worked in substantially the same manner as traditional sterlings. Pieces cast from the improved alloy may be age hardened to about 160 Vickers, if desired.

Abstract: A jewelry alloy is disclosed preferably of 1 to 5 karat gold having the color, hue and shine characteristics of 10 or higher karat yellow gold. Although the alloy disclosed is virtually identical in appearance to 10 to 18 karat yellow gold, it is substantially less expensive and provides a relatively low cost substitute for higher karat yellow gold. The alloy has the following composition, by weight: Gold: 17-25% Silver: 10-27% Copper: 40-60% Zinc: 3-12% In addition to its appearance, the alloy disclosed has good corrosion and high tarnish resistance, good workability and castability and can be plated, if desired, and sized with conventional solders.

Abstract: A copper alloy according to the present invention includes 17 mass % to 34 mass % of Zn, 0.02 mass % to 2.0 mass % of Sn, 1.5 mass % to 5 mass % of Ni, and a balance consisting of Cu and unavoidable impurities, in which relationships of 12?f1=[Zn]+5×[Sn]?2×[Ni]?30, 10?[Zn]?0.3×[Sn]?2×[Ni]?28, 10?f3={f1×(32?f1)×[Ni]}1/2?33, 1.20?0.7×[Ni]+[Sn]?4, and 1.4?[Ni]/[Sn]?90 are satisfied, conductivity is 13% IACS to 25% IACS, a ratio of an ? phase is 99.5% or more by area ratio or an area ratio of a ? phase (?)% and an area ratio of a ? phase (?)% in an ? phase matrix satisfy a relationship of 0?2×(?)+(?)?0.7.

Abstract: A copper alloy according to the present invention includes 17 mass % to 34 mass % of Zn, 0.02 mass % to 2.0 mass % of Sn, 1.5 mass % to 5 mass % of Ni, and a balance consisting of Cu and unavoidable impurities, in which relationships of 12?f1=[Zn]+5×[Sn]?2×[Ni]?30, 10?[Zn]?0.3×[Sn]?2×[Ni]?28, 10?f3={f1×(32?f1)×[Ni]}1/2?33, 1.2?0.7×[Ni]+[Sn]?4, and 1.4?[Ni]/[Sn]?90 are satisfied, conductivity is 13% IACS to 25% IACS, a ratio of an ? phase is 99.5% or more by area ratio or an area ratio of a ? phase (?)% and an area ratio of a ? phase (?)% in an ? phase matrix satisfy a relationship of 0?2×(?)+(?)?0.7.

Abstract: Provided is a multilayer electrical steel sheet having both low high-frequency iron loss and high magnetic flux density. The multilayer electrical steel sheet has an inner layer and surface layers provided on both sides of the inner layer, in which the surface layers and the inner layer have a predetermined chemical composition, the multilayer electrical steel sheet having: ?Si of 0.5 mass % to 4.0 mass %; ?Al of 0.05 mass % or less; a ratio of ti to t represented by ti/t of from 0.10 to 0.70; a magnetic flux density Bio of 1.3 T or more; and a ratio of B1 to B10 represented by B1/B10 of 0.45 or more; and an iron loss W10/1k in W/kg and the sheet thickness t in mm satisfy the following formula (1): W10/1k?15+140×t??(1).

Abstract: Provided is a ferritic stainless steel sheet excellent in shape of weld zone and corrosion resistance of a weld zone with a material of a different kind formed by performing welding with austenitic stainless steel. A ferritic stainless steel sheet having a chemical composition containing, by mass %, C: 0.003% to 0.020%, Si: 0.01% to 1.00%, Mn: 0.01% to 0.50%, P: 0.040% or less, S: 0.010% or less, Cr: 20.0% to 24.0%, Cu: 0.20% to 0.80%, Ni: 0.01% to 0.60%, Al: 0.01% to 0.08%, N:. 0.003% to 0.020%, Nb: 0.40% to 0.80%, Ti: 0.01% to 0.10%, Zr: 0.01% to 0.10%, and the balance being Fe and inevitable impurities, in which relational expression (1) below is satisfied: 3.0?Nb/(2Ti+Zr+0.5 Si+5 Al)?1.5 ??(1), here, in relational expression (1), each of the atomic symbols denotes the content (mass %) of the corresponding chemical element.

Abstract: A nickel-containing steel for low temperature according to an aspect of the present invention has a chemical composition within a predetermined range, in which a metallographic structure of a thickness middle portion contains 2.0 vol % to 20.0 vol % of an austenite phase, an average grain size of prior austenite grains is 3.0 ?m to 15.0 ?m, an average aspect ratio of the prior austenite grains is 1.0 to 2.4, a plate thickness is 4.5 mm to 30 mm, the chemical composition and the average grain size of the prior austenite grains are further limited depending on the plate thickness, a yield stress at room temperature is 460 MPa to 710 MPa, and a tensile strength at the room temperature is 560 MPa to 810 MPa.

Abstract: Described is a hot-rolled steel having a tensile strength of at least 950 MPa and a microstructure that includes bainite at an area ratio of 70% or more; the balance being: martensite at an area ratio of 30% or less, and optionally ferrite at an area ratio of 20% or less. The hot-rolled steel has a chemical composition containing (in mass-%): C: 0.07-0.10, Si: 0.01-0.25, Mn: 1.5-2.0, Cr: 0.5-1.0, Ni: 0.1-0.5, Cu: 0.1-0.3, Mo: 0.01-0.2, Al: 0.01-0.05, Nb: 0.015-0.04, V: 0-0.1, i.e. optionally up to 0.1 mass-% Vanadium, Ti: 0-0.1, whereby the balance is Fe and unavoidable impurities.

Abstract: An amorphous alloy particle is an amorphous alloy particle formed of an iron-based alloy, and the particle contains a grain boundary layer.

Abstract: The present invention relates to extrusions for structural components, such as bumper, side impact beam, seat sill in vehicles and more particularly to a method for optimizing strength and energy absorption of 6XXX aluminium alloys extrusions by variations in thermomechanical ageing (TMA) consisting in i) an artificial preageing treatment with a duration t1 at a temperature T1 selected to increase the yield strength of said extrusion between 5% and 20%, said temperature T1 being typically between 120° C. and 180° C. and said duration t1 being typically between 1 and 100 hours, to obtain an artificially preaged extrusion, ii) a plastic deformation of said artificially preaged extrusion between 1% and 80% to obtain a deformed extrusion, iii) a final artificial ageing treatment of said deformed extrusion with a duration t2 at a temperature T2, said temperature T2 being typically between 140° C. and 200° C. and said the duration t2 being typically between 1 and 100 hours.