Patents Issued in December 31, 2020
  • Publication number: 20200407727
    Abstract: The invention provides compositions and methods of making and using effector oligonucleotides, including effector oligonucleotides with greater than one mismatch as compared to its target sequence. These effector oligonucleotides are useful for improving the efficiency of genomic editing as well as providing therapeutic benefits to individuals in need thereof.
    Type: Application
    Filed: April 24, 2020
    Publication date: December 31, 2020
    Inventor: Kambiz SHEKDAR
  • Publication number: 20200407728
    Abstract: The present invention relates to compositions and methods for generating a modified T cell with a nucleic acid capable of downregulating endogenous gene expression selected from the group consisting of TCR ? chain, TCR ? chain, beta-2 microglobulin, a HLA molecule, CTLA-4, PD1, and FAS and further comprising a nucleic acid encoding a modified T cell receptor (TCR) comprising affinity for a surface antigen on a target cell or an electroporated nucleic acid encoding a chimeric antigen receptor (CAR). Also included are methods and pharmaceutical compositions comprising the modified T cell for adoptive therapy and treating a condition, such as an autoimmune disease.
    Type: Application
    Filed: July 30, 2020
    Publication date: December 31, 2020
    Inventors: Yangbing Zhao, Jiangtao Ren, Xiaojun Liu, Carl H. June
  • Publication number: 20200407729
    Abstract: The present application provides materials and methods for controlling gene editing. The present application also provides materials and methods for controlling transcriptional expression of guide RNAs and/or post-transcriptional expression of Cas nuclease.
    Type: Application
    Filed: June 26, 2020
    Publication date: December 31, 2020
    Inventors: Ryo TAKEUCHI, Abraham SCARIA
  • Publication number: 20200407730
    Abstract: The present invention relates to a promoter system inducing expression of 3-hydroxypropionic acid (3-HP) and a method of biologically producing 3-HP using the same. To improve production of 3-HP in a biological manner, continuous synthesis of new enzymes having enzyme activity is necessary. As a result of screening 3-HP reactive transcription regulators and 3-HP reactive promoters from several microorganisms including Pseudomonas denitrificans, it was confirmed that the transcriptions regulations and promoters are composed of LysR proteins and particular gene nucleotide sequences binding to the LysR proteins. Therefore, the 3-HP inducible system is expected to be effectively used to regulate 3-HP metabolic pathways.
    Type: Application
    Filed: September 11, 2020
    Publication date: December 31, 2020
    Applicants: NOROO IC Co., Ltd., NOROO R&C CO., LTD.
    Inventors: Sung-Hoon PARK, Shengfang ZHOU, Somasundar ASHOK, Eun Hee SEOL, Satish Kumar AINALA
  • Publication number: 20200407731
    Abstract: Provided are a novel promoter and a method of producing L-amino acid using the same.
    Type: Application
    Filed: March 15, 2019
    Publication date: December 31, 2020
    Inventors: Jee Yeon BAE, Chang II SEO, Inhwa YOO, Hye Ryun YOO, So Young KIM, Yong Uk SHIN
  • Publication number: 20200407732
    Abstract: The present disclosure provides methods to increase the percentage of edited yeast cells in a cell population using nucleic-acid guided editing, and automated multi-module instruments for performing these methods.
    Type: Application
    Filed: June 17, 2020
    Publication date: December 31, 2020
    Inventors: Krishna Kannan, Miles Gander, Eileen Spindler, Paul Hardenbol
  • Publication number: 20200407733
    Abstract: The invention relates to a recombinant yeast comprising a nucleotide sequence allowing the expression of a glucoamylasey (EC 3.2.1.20 or 3.2.1.3). This cell can be used for the production of ethanol and advantageously produces little or no glycerol.
    Type: Application
    Filed: September 25, 2018
    Publication date: December 31, 2020
    Inventors: Hans Marinus Charles Johannes DE BRUIJN, Paulus Petrus DE WAAL, Ingrid Maria VUGT-VAN LUTZ
  • Publication number: 20200407734
    Abstract: Described are compositions and methods relating to modified yeast that over-express polyadenylate-binding protein (PAB1). The yeast produces a deceased amount of acetate compared to parental cells. Such yeast is particularly useful for large-scale ethanol production from starch substrates where acetate in an undesirable end product.
    Type: Application
    Filed: March 4, 2019
    Publication date: December 31, 2020
    Inventors: Min Qi, Paula Johanna Maria Teunissen, Quinn Qun Zhu
  • Publication number: 20200407735
    Abstract: A vector includes one or more promoters. The promoters are expressed specifically in a cottonseed surface or a cotton fiber or both of the cottonseed surface and the cotton fiber.
    Type: Application
    Filed: August 1, 2018
    Publication date: December 31, 2020
    Applicants: NIPPON STEEL TRADING CORPORATION, NATIONAL UNIVERSITY CORPORATION TOKYO UNIVERSITY OF AGRICULTURE AND TECHNOLOGY
    Inventor: Sakae Suzuki
  • Publication number: 20200407736
    Abstract: The present invention provides a soybean genetic transformation method using PMI as selectable gene, and relates to the technical field of genetic engineering. In the soybean genetic transformation method of the present invention, using the PMI gene as a selectable marker, soybean explants are infected by recombinant Agrobacterium with the PMI gene and a target gene, followed by co-culture; without recovery culture, the co-cultured explants are directly selected by a selective medium supplemented with mannose, where transformed explants with the PMI gene grow normally under selection pressure of mannose, while non-transformed explant growth is inhibited, thereby selecting successfully transformed positive plants; after shoot elongation and transplantation of the positive plants obtained, genetically transformed soybean plants are obtained successfully.
    Type: Application
    Filed: June 25, 2020
    Publication date: December 31, 2020
    Applicant: Institute of Crop Sciences, Chinese Academy of Agricultural Sciences
    Inventors: LIJUAN QIU, Ying Wang
  • Publication number: 20200407737
    Abstract: The present invention relates to the use of CRISPR/CasX systems in plants for genome engineering, and compositions used in such methods.
    Type: Application
    Filed: May 3, 2018
    Publication date: December 31, 2020
    Applicant: KWS SAAT SE & Co. KGaA
    Inventors: Aaron HUMMEL, Zarir VAGHCHHIPAWALA
  • Publication number: 20200407738
    Abstract: This disclosure provides an engineered system comprising: a first nucleic acid molecule encoding a CasX nuclease, and a guide RNA (gRNA) or a second nucleic acid molecule encoding the gRNA, where the first nucleic acid molecule is codon optimized for a eukaryotic cell, and where the gRNA is designed to hybridize with a target site in the eukaryotic cell. Further, this disclosure provides a method of modifying at least one target site in a eukaryotic genome comprising: providing a eukaryotic cell with a CasX nuclease or a first nucleic acid molecule encoding the CasX nuclease, and providing the eukaryotic cell with a guide RNA (gRNA) or a second nucleic acid molecule encoding the gRNA, where the gRNA and the CasX nuclease form a complex, where the gRNA hybridizes to the target site, and where the complex generates a modification at the target site.
    Type: Application
    Filed: October 24, 2018
    Publication date: December 31, 2020
    Applicant: MONSANTO TECHNOLOGY LLC
    Inventor: Ervin D. NAGY
  • Publication number: 20200407739
    Abstract: This disclosure concerns synthetic polynucleotides encoding a polypeptide of interest that are particularly well-suited for expression in target plants.
    Type: Application
    Filed: September 15, 2020
    Publication date: December 31, 2020
    Inventors: Aaron Woosley, Sarah Worden
  • Publication number: 20200407740
    Abstract: The subject invention concerns materials and methods for increasing and/or improving photosynthetic efficiency in plants, and in particular, C3 plants. In particular, the subject invention provides for means to increase the number of bundle sheath (BS) cells in plants, to improve the efficiency of photosynthesis in BS cells, and to increase channels between BS and mesophyll (M) cells. In one embodiment, a method of the invention concerns altering the expression level or pattern of one or more of SHR, SCR, and/or SCL23 in a plant. The subject invention also pertains to genetically modified plants, and in particular, C3 plants, that exhibit increased expression of one or more of SHR, SCR, and/or SCL23. Transformed and transgenic plants are contemplated within the scope of the invention.
    Type: Application
    Filed: August 31, 2020
    Publication date: December 31, 2020
    Inventors: HONGCHANG CUI, DANYU KONG, YUELING HAO
  • Publication number: 20200407741
    Abstract: The present disclosure relates to plant-based recombinant protein production systems and their methods of production and use. The plant-based recombinant protein production system is a vector comprising a 5? UTR and a 3? UTR, wherein the 3? UTR comprises at least one terminator selected from the group consisting of: EU, IEU, NbACT3, NbACT617, NbACT567, Pin2, BDB501, BDB282, NbHSP, NbHSPb, Rep, RbcS, SIR, SIR 5?/3?, SIR 3?, AtHSP, 35S, RepA, NOS, TMV, TNVD, PEMV, and BYDV. In certain implementations, the vector comprises two terminators in the 3? UTR, where the two terminators are fused to form a double terminator. For example, the double terminator comprises two members selected from the group consisting of: EU, IEU, NbACT3, NbACT617, NbACT567, Pin2, BDB501, BDB282, NbHSP, NbHSPb, Rep, RbcS, SIR, SIR 5?/3?, SIR 3?, AtHSP, 35S, RepA, NOS, TMV, TNVD, PEMV, and BYDV. In some aspects, the vector further comprises a chromatin scaffold/matrix attachment region (MAR) that is downstream of the terminators.
    Type: Application
    Filed: March 4, 2019
    Publication date: December 31, 2020
    Inventors: Hugh Mason, Andrew Diamos, Sun Hee Rosenthal
  • Publication number: 20200407742
    Abstract: Provided are compositions and methods for expressing a transgene in plant cells and/or plant tissues using regulatory elements, including the promoters, 5?UTR, 3? UTRs, and/or terminators isolated from Glycine max chlorophyll binding Ab genes.
    Type: Application
    Filed: August 31, 2018
    Publication date: December 31, 2020
    Inventors: LYUDMILA SIDORENKO, SCOTT ALAN BEVAN, CORY M LARSEN, GENY I ANTHONY, ANDREW E ROBINSON, CARLA N YERKES
  • Publication number: 20200407743
    Abstract: The present disclosure provides compositions and methods for providing broad-based resistance to fungal pathogens, such as a Fusarium fungi, and plants derived therefrom.
    Type: Application
    Filed: June 9, 2020
    Publication date: December 31, 2020
    Inventor: Walter MESSIER
  • Publication number: 20200407744
    Abstract: Green bean plants exhibiting resistance to Sclerotinia sclerotiorum are provided, together with kits and methods for controlling Sclerotinia sclerotiorum infection. Such methods include the application of fungicidal Bacillus subtilis strains to plants exhibiting resistance to Sclerotinia sclerotiorum.
    Type: Application
    Filed: July 15, 2020
    Publication date: December 31, 2020
    Inventors: Ellen L. Evans, Kenneth Kmiecik, Chad Kramer, Arie Oppelaar
  • Publication number: 20200407745
    Abstract: An attenuated strain of cucumber green mottle mosaic virus (CGMMV) is useful to protect cucumber plants from infection with the wild-type infectious CGMMV strain. The genome of the attenuated virus contains at least one mutation or group of mutations selected from c.4969G>A, c.3334C>T, and a group of at least six of the mutations c.315G>A; c.1498A>G; c.1660C>T; c.3430C>T; c.3528A>G; c.4144C>T; c.4248C>T; and c.6228C>T. These mutated genomes encode one or more mutations selected from R163711 in the 186 kDa readthrough replication protein, A1092V in the 129 kDa replication protein and/or the 186 kDa readthrough replication protein, and at least six mutations selected from G86S, E480G, S534F, A1124V, N1157D, P1362L, P1397S in the 129 kDa replication protein and/or the 186 kDa readthrough replication protein, and the A156V mutation in the coat protein.
    Type: Application
    Filed: September 11, 2020
    Publication date: December 31, 2020
    Inventors: Keri WANG, George LAZAROVITS, Yibin LIU, Magda KONOPKA, Greg PATTERSON
  • Publication number: 20200407746
    Abstract: The present invention relates to nucleic acid regulatory elements that are able to enhance muscle-specific expression of genes, in particular expression in cardiac muscle and/or skeletal muscle, methods employing these regulatory elements and uses of these elements. Expression cassettes and vectors containing these nucleic acid regulatory elements are also disclosed. The present invention is particularly useful for applications using gene therapy, more particularly muscle-directed gene therapy, and for vaccination purposes.
    Type: Application
    Filed: July 9, 2020
    Publication date: December 31, 2020
    Inventors: Thierry Vandendriessche, Marinee Chuah, Pieter De Bleser
  • Publication number: 20200407747
    Abstract: Some embodiments of the methods and compositions provided herein relate to the preparation surfactant protein-D (SP-D). Some embodiments include the expression of human SP-D in certain cell lines, and the purification of human SP-D from such cell lines. Some embodiments include the preparation of certain oligomeric forms of human SP-D.
    Type: Application
    Filed: July 16, 2020
    Publication date: December 31, 2020
    Inventors: Jan Susan Rosenbaum, Frederick Gyapon Quast, Matthias Kaup, Lars Stöckl
  • Publication number: 20200407748
    Abstract: The invention provides a splice control module for sex-specific splicing and expression of a gene of interest. In certain embodiments, a dsx-based splice control module is used to express a lethal gene in an insect that is spliced in a sex-specific manner to impart lethality to female insects but not male insects.
    Type: Application
    Filed: August 9, 2017
    Publication date: December 31, 2020
    Applicant: OXITEC LTD.
    Inventors: Luke ALPHEY, Tarig DAFA'ALLA, Amandine COLLADO, Simon WARNER, Kelly MATZEN, Sian SPINNER
  • Publication number: 20200407749
    Abstract: The present invention relates to nucleic acid regulatory elements that are able to enhance diaphragm-specific expression of genes, in particular expression in diaphragm as such, or in combination with expression in cardiac muscle and/or skeletal muscle, methods employing these regulatory elements and uses of these elements. Expression cassettes and vectors containing these nucleic acid regulatory elements are also disclosed. The present invention is particularly useful for applications using gene therapy, more particularly diaphragm-directed gene therapy, and for vaccination purposes.
    Type: Application
    Filed: March 27, 2018
    Publication date: December 31, 2020
    Applicant: VRIJE UNIVERSITEIT BRUSSEL
    Inventors: Lay Khim CHUAH, Thierry VANDENDRIESSCHE, Warut TULALAMBA
  • Publication number: 20200407750
    Abstract: A recombinant adeno-associated virus (rAAV) vector comprising an AAV capsid having a heterogeneous population of vp1 proteins, a heterogeneous population of vp2 protein and a heterogeneous population of vp3 proteins. The capsid contains modified amino acids as compared to the encoded VP1 amino acid sequence, the capsid containing highly deamidated asparagine residues at asparagine-glycine pair, and further comprising multiple other, less deamidated asparagine and optionally glutamine residues. Methods of reducing deamidation in the AAV capsid of a rAAV are provided.
    Type: Application
    Filed: February 27, 2019
    Publication date: December 31, 2020
    Inventors: James M. Wilson, April Tepe, Kevin Turner, Joshua Joyner Sims
  • Publication number: 20200407751
    Abstract: In one embodiment, the invention provides an Adeno-Associated Virus (AAV) comprising an exterior surface, which surface comprises one or more peptide tags that form a bond with a binding-partner, wherein the AAV is a live virus. In another embodiment, the invention provides a conjugate comprising at least one such AAV and at least one polypeptide comprising a first domain which is the binding-partner for the tag and a second domain, which is a bioactive polypeptide. In another embodiment, the invention provides a conjugate comprising at least one such AAV (first AAV) and at least one second AAV, which second AAV comprises a second exterior surface, which second exterior surface comprises at least one binding-partner for the tag or for a third linker molecule, wherein the at least one first AAV and the at least one second AAV are bound.
    Type: Application
    Filed: February 28, 2019
    Publication date: December 31, 2020
    Inventors: Leah BYRNE, Bilge Esin OZTURK, Timothy DAY
  • Publication number: 20200407752
    Abstract: The present invention provides a method for producing microvesicles comprising a transgene product and/or a lentiviral RNA comprising a transgene, comprising the steps of: culturing a cell into which the transgene has been introduced using a lentiviral vector in vitro to extracellularly release microvesicles comprising the transgene product and/or the lentiviral RNA comprising the transgene, wherein said lentiviral vector is deficient in at least one structural protein gene and comprises the transgene under control of a telomerase reverse transcriptase (TERT) gene promoter in a lentiviral genome sequence, and collecting the microvesicles released; and a microvesicle obtained according to this method and its use.
    Type: Application
    Filed: August 24, 2020
    Publication date: December 31, 2020
    Inventors: Zhong LI, Misako KATSURA
  • Publication number: 20200407753
    Abstract: Provided herein are compositions and methods for packaging a recombinant adeno-associated virus (rAAV) particle comprising using inverted terminal repeats (ITRs) and rep genes of different serotypes and/or using chimeric rep genes.
    Type: Application
    Filed: March 6, 2019
    Publication date: December 31, 2020
    Applicant: University of Florida Research Foundation, Incorporated
    Inventors: Mavis Agbandje-McKenna, Mario Mietzsch, Robert McKenna
  • Publication number: 20200407754
    Abstract: Eukaryotic cells and related reagents, systems, methods, and compositions for increasing the frequency of homology directed repair (HDR) of target editing sites with genome editing molecules are provided.
    Type: Application
    Filed: June 24, 2020
    Publication date: December 31, 2020
    Applicant: Inari Agriculture, Inc.
    Inventor: Tomás Cemák
  • Publication number: 20200407755
    Abstract: Methods and compositions for introducing genetic mutations into non-human animal cells are provided. These cells can be used to produce animal models of human disease. In some embodiments, the genetic mutations are flanked by DNA sequences that are “humanized” to match homologous DNA sequences. In some embodiments, the animal model is a large mammalian model for an inherited metabolic disorder. In some embodiments, the animal model is a pig model for phenylketonuria (PKU) created by introducing a missense mutation into exon 8 of the Pah gene.
    Type: Application
    Filed: April 30, 2020
    Publication date: December 31, 2020
    Inventors: Joseph Lillegard, Daniel F. Carlson, Cary O. Harding, Dennis Webster
  • Publication number: 20200407756
    Abstract: The present invention is directed to methods for catalyzing a chemical reaction by retroaldolases, corresponding uses of retroaldolases and to novel retroaldolases. The methods and retroaldolases have utility in (i) preparing tertiary alcohols, in (ii) chiral resolution of tertiary alcohols by retroaldol cleavage, and in (iii) deuteration or tritiation of carbonyl compounds.
    Type: Application
    Filed: February 12, 2019
    Publication date: December 31, 2020
    Inventors: DONALD MICHAEL HILVERT, XAVIER GARRABOU PI, DUNCAN STUART MACDONALD
  • Publication number: 20200407757
    Abstract: Provided herein is a method for producing alcohol. The method comprises fermenting milk permeate with yeast to produce a fermented broth comprising the ethanol. The fermented broth is then subjected to distillation to obtain a concentrated ethanol-enriched vapour having at least 50% v/v ethanol. From the ethanol-enriched vapour resulting from the distillation, an ethanol product is produced that is a biofuel or a potable spirit. The disclosure also provides a unique potable spirit composition produced from such process.
    Type: Application
    Filed: April 1, 2020
    Publication date: December 31, 2020
    Inventor: Omid McDonald
  • Publication number: 20200407758
    Abstract: The present disclosure relates to alpha-amylases for use in combination with glucoamylases for improving the hydrolysis of a raw starch. The alpha-amylases can be provided in a purified form and/or can be expressed from a recombinant host cell. The present disclosure also provides a population of recombinant host cells expressing the alpha-amylases to be used in combination with recombinant host cells expressing the glucoamylases.
    Type: Application
    Filed: June 30, 2017
    Publication date: December 31, 2020
    Inventors: Ryan Skinner, Charles F. Rice, Aaron Argyros
  • Publication number: 20200407759
    Abstract: The present invention relates to an improved method of second generation ethanol production from a lignocellulosic biomass. The process comprises subjecting a slurry of pre-treated lignocellulosic biomass comprising C5 and C6 sugars in a fermentor; preferentially fermenting mainly C5 sugars by incubating the pretreated lignocellulosic biomass of step (i) with first cellulase enzyme, co-fermenting microorganism, nutrient in the fermentor at 30-37° C. for a period of 16 to 24 hours; adding second cellulase enzyme to the fermentation broth of step (ii) and increasing the temperature to 45-55° C. for hydrolysis; allowing the fermentation broth of step (iii) to cool to a temperature of 35-38° C.; and preferentially fermenting C6 sugars by incubating the broth of step (iv) with a second dose of co-fermenting microorganism for a period of 6-8 hours to obtain ethanol. The process results in high ethanol productivity in shortest duration of time.
    Type: Application
    Filed: June 24, 2020
    Publication date: December 31, 2020
    Inventors: Ajay Kumar Sharma, Manas Ranjan Swain, Ajit Singh, Anshu Shankar Mathur, Ravi Prakash Gupta, Suresh Kumar Puri, Sankara Sri Venkata Ramakumar
  • Publication number: 20200407760
    Abstract: Aqueous fermentation feedstock and method of producing same. The feedstock includes glucose and dextrose oligomers, wherein (i) glucose concentration is in a range between 10 gram/Liter (g/L) and 150 g/L; (ii) dextrose oligomers concentration is in a range between 50 g/L and 300 g/L; and optionally (iii) slurried particles of less than 0.5 micron; (iv) slurried particles of more than 0.5 micron, wherein a content of such suspended particles of more than 0.5 micron is less than 30 g/L; (v) ash at a concentration in a range between 20 g/L and 50 g/L; (vi) lactate at a concentration in a range between 0.5 g/L and 10 g/L; (vii) protein at a concentration in a range between 5 g/L and 50 g/L; (viii) corn oil at a concentration of less than 10 g/L; and/or (ix) glycerol at a concentration in a range between 1 g/L and 30 g/L.
    Type: Application
    Filed: September 10, 2020
    Publication date: December 31, 2020
    Inventors: Bryan P. TRACY, John Randall PHILLIPS, Daniel Knox MITCHELL
  • Publication number: 20200407761
    Abstract: The present invention relates to at least one cell for producing at least one lipid with general formula II from at least one carbon substrate, wherein R1 and R2 independently of one another comprises identical or different organic radicals each with 5 to 13 carbon atoms, wherein the cell is a non-pathogenic cell that is genetically modified to increase the heterologous expression relative to the wild type cell of: an enzyme (E2) capable of converting 3-hydroxyalkanoyl-3-hydroxyalkanoyl-CoA/ACP or 3-(3-hydroxyalkanoyloxy)alkanoic acid (HAA) and NDP-glucose into ?-D-glucopyranosyl-3-hydroxyalkanoyl-3-hydroxyalkanoate.
    Type: Application
    Filed: February 8, 2019
    Publication date: December 31, 2020
    Applicant: EVONIK OPERATIONS GMBH
    Inventors: Mirja WESSEL, Steffen SCHAFFER, Anne JEREMIAS, Martin SCHILLING, Hans Henning WENK
  • Publication number: 20200407762
    Abstract: Provided is a novel production system that does not involve, or can minimize, the transport of liquid ammonia in the production of an organic compound or the production of a microorganism by microbial fermentation. A production system for an organic compound or a microorganism includes: an ammonia synthesis apparatus in which an ammonia-containing gas is synthesized by reaction of a source gas containing hydrogen and nitrogen in the presence of a supported ruthenium catalyst; and a culture apparatus that cultures a microorganism having organic compound productivity using ammonia originating from the ammonia-containing gas obtained by using the ammonia synthesis apparatus.
    Type: Application
    Filed: September 14, 2020
    Publication date: December 31, 2020
    Applicants: AJINOMOTO CO., INC., TOKYO INSTITUTE OF TECHNOLOGY
    Inventors: Mitsuhiro Kishino, Hiroyuki Kojima, Hideo Hosono, Michikazu Hara, Masaaki Kitano, Toshiharu Yokoyama, Toru Numaguchi, Munenobu Ito, Kazuteru Yamada, Hiromi Noguchi
  • Publication number: 20200407763
    Abstract: A method for the production of pullulan soft capsules eliminates the need to dry pullulan solid product, thereby reducing the equipment cost and energy consumption. The pullulan raw material production can be linked directly with the soft capsule production to provide a unique approach for soft capsule formation. The purified pullulan fermentation fluid can be directly used in soft capsule preparation, thus removing the need for a melting process. On the one hand, the method can decrease material consumption, save the cost of equipment and labor, reduce production time and increase productivity. On the other hand, the method can reduce the fluctuating of raw material quality in the re-melting process and guarantee a more stable soft capsule production and quality.
    Type: Application
    Filed: June 26, 2019
    Publication date: December 31, 2020
    Inventors: Xianyu YUN, Sheng WANG, Zhongming FANG, Fei WANG, Peiyong LIU, Xiaosan CAO, Zhidong LU
  • Publication number: 20200407764
    Abstract: The present disclosure relates to a method for preparing a fermented composition, and more specifically, to a method for preparing a fermented composition with improved odor, which comprises preparing grain flour; performing primary fermentation of the grain flour using yeast; performing secondary fermentation of the primary fermented product using a strain of the genus Bacillus; and obtaining the secondary fermented product. The fermented composition of the present disclosure has a high content of a peptide with a low molecular weight, and thus enables the increase of digestibility and absorption rate of proteins during ingestion while also improving the peculiar odor of a fermented product to enhance its palatability.
    Type: Application
    Filed: February 14, 2019
    Publication date: December 31, 2020
    Applicant: CJ CHEILJEDANG CORPORATION
    Inventors: HyoJeong Seo, Tae Joo Yang, Hyun Chi, Myeong-hyeon Choi, Seung Won Park, YoungHo Hong
  • Publication number: 20200407765
    Abstract: A cell lysate or cell-free protein synthesis system comprising recombinant translational proteins and uses thereof are provided.
    Type: Application
    Filed: June 29, 2020
    Publication date: December 31, 2020
    Inventors: Cheemeng TAN, Luis Eduardo Contreras LLANO, Conary MEYER, Fernando VILLARREAL
  • Publication number: 20200407766
    Abstract: The present invention relates to a coupled biotransformation process of converting chenodeoxycholic acid (CDCA) and related compounds to ursodeoxycholic acid (UDCA) and related compounds. It also relates to the cloning, expression, and biochemical characterization of a novel NADP+-dependent 7?-hydroxysteroid dehydrogenase (7?-HSDH) from Clostridium difficile, cofactor switch mutants thereof, and their application for the oxidation of bile acids. A further aspect of the invention relates to novel NADP-dependent cofactor switch mutants of the NADP+-dependent 7?-HSDH of E. coli and their application for the oxidation of bile acids.
    Type: Application
    Filed: June 19, 2017
    Publication date: December 31, 2020
    Inventors: Daniel Bakonyi, Werner Hummel, Ralf Gross
  • Publication number: 20200407767
    Abstract: One aspect of the invention provides a nucleic acid encoding a 7?-hydroxysteroid dehydrogenase (7?-HSDH) that catalyzes at least the stereospecific enzymatic reduction of a 7-ketosteroid to the corresponding 7-hydroxysteroid. The enzyme includes a mutation at position 64 of SEQ ID NO:2 or in the corresponding sequence positions of an amino acid sequence derived therefrom with at least 90% sequence identity to SEQ ID NO:2. The mutation at position 64 is the mutation R64X1, wherein X1 represents E, D, T, L, S, P, V, K, C, A, G, Q, F, W, I or Y. The enzyme shows the following property profile in comparison with the 7?-HSDH with SEQ ID NO:2: (a) an increased specific activity (Vmax [U/mg]) for NADPH in the enzymatic reduction of dehydrocholic acid (DHCA) with NADPH as cofactor.
    Type: Application
    Filed: July 10, 2020
    Publication date: December 31, 2020
    Applicant: Pharmazell GmbH
    Inventors: Werner Hummel, Daniel Bakonyi
  • Publication number: 20200407768
    Abstract: It is an object of an aspect of the present invention to provide (i) transformants, whose proliferation depends on phosphite, of various species of organism and (ii) a method for detecting the presence of a reduced phosphorous compound with use of such a transformant. Use is made of a transformant which is defective in functions of a gene encoding a phosphate transporter protein and a gene encoding a phosphate ester transporter protein and into which a gene encoding a hypophosphite transporter protein is introduced, a signal peptide of a hypophosphite binding protein being substituted with a signal peptide derived from a host or a species of organism closely related to the host.
    Type: Application
    Filed: March 1, 2019
    Publication date: December 31, 2020
    Inventors: Ryuichi HIROTA, Akio KURODA, Kei MOTOMURA
  • Publication number: 20200407769
    Abstract: Provided is a technique that can be used to detect a pathogenic microorganism such as influenza virus with high sensitivity, and specifically, a method for detecting a reaction product, including reacting an enzyme with a substance serving as a substrate for a reaction involving the enzyme in a hydrophilic solvent that is in interfacial contact with a hydrophobic solvent, wherein the reaction product is produced directly and released from the substrate, and wherein the hydrophilic solvent contains at least one of a buffering substance or trimethylamine oxide (TMAO).
    Type: Application
    Filed: March 4, 2019
    Publication date: December 31, 2020
    Inventors: Kazuhito Tabata, Yoshihiro Minagawa, Hiroyuki Noji
  • Publication number: 20200407770
    Abstract: The present invention relates to a method for detecting the presence of expanded-spectrum ?-lactamase (?-lactamase hydrolyzing expanded-spectrum cephalosporin)-producing bacteria in a sample, said method comprising the steps of: a) performing cell lysis on a test sample in order to obtain an enzymatic suspension; b) reacting a fraction of the enzymatic suspension obtained in step a) with a reagent kit, said reagent kit comprising—expanded-spectrum ?-lactamase substrate selected from the group consisting of cephalosporins, aztreonam, and cephamycins, —a pH color indicator which will change color when the pH of the reaction mixture is comprised between 6.4 and 8.4, wherein a color change after step b) indicates the presence of expanded-spectrum ?-lactamase-producing bacteria in the test sample. The invention also relates to a reagent kit, to a microtiter plate and to their uses in detecting the presence of expanded-spectrum ?-lactamase producers in a test sample.
    Type: Application
    Filed: September 3, 2020
    Publication date: December 31, 2020
    Inventors: Patrice NORDMANN, Laurent DORTET, Laurent POIREL
  • Publication number: 20200407771
    Abstract: Provided is a diagnostic test for the positive identification of patients with bradykinin-mediated angioedema. The test is valuable for distinguishing bradykinin-mediated angioedema from non-bradykinin-mediated angioedema. The test allows clinicians to clearly separate patients with bradykinin-mediated from histamine-mediated angioedema. Results can be obtained in under an hour, allowing for the proper treatment of angioedema based on the underlying etiology.
    Type: Application
    Filed: February 22, 2019
    Publication date: December 31, 2020
    Inventors: Bruce Zuraw, Maria Luz Lara-Marquez
  • Publication number: 20200407772
    Abstract: Provided herein are methods of determining NAT acetylation status of a subject with a 3,4-DAP-sensitive disease, methods of selecting a dose of 3,4-DAP or a pharmaceutically acceptable salt thereof adjusted to a subject's acetylation status, methods of administering 3,4-diaminopyridine or a pharmaceutically acceptable salt thereof to a patient in need thereof, and methods of treating 3,4-DAP sensitive diseases.
    Type: Application
    Filed: September 1, 2020
    Publication date: December 31, 2020
    Inventors: Marvin R. GAROVOY, Peter E. HAROLDSEN, Donald G. MUSSON
  • Publication number: 20200407773
    Abstract: Provided herein are methods of determining NAT acetylation status of a subject with a 3,4-DAP-sensitive disease, methods of selecting a dose of 3,4-DAP or a pharmaceutically acceptable salt thereof adjusted to a subject's acetylation status, methods of administering 3,4-diaminopyridine or a pharmaceutically acceptable salt hereof to a patient in need thereof, and methods of treating 3,4-DAP sensitive diseases.
    Type: Application
    Filed: September 1, 2020
    Publication date: December 31, 2020
    Inventors: Marvin R. GAROVOY, Peter E. HAROLDSEN, Donald G. MUSSON
  • Publication number: 20200407774
    Abstract: The present disclosure provides a nucleic acid homogenization method, and a kit and use thereof. The method including at least the following steps: respectively adding nucleic acid adsorption materials having the same nucleic acid saturation adsorption amount into a plurality of nucleic acid solutions, and the nucleic acid adsorption materials added into each nucleic acid solution can all achieve a nucleic acid adsorption saturation state; separating the nucleic acid adsorption materials of the saturation absorbed nucleic acids; eluting the nucleic acids from the separated nucleic acid adsorption materials. The nucleic acid homogenization method disclosed in the present disclosure is easy to operate, and allows for rapid and stable equal proportional dilution of a nucleic acid, a PCR product or a high throughput sequencing library concentration.
    Type: Application
    Filed: August 8, 2017
    Publication date: December 31, 2020
    Applicant: Shanghai ZJ Bio-Tech Co., Ltd.
    Inventors: Jie ZHANG, Qiang LI, Kai WANG, Ning WANG, Jingyi NI, Zhongying LU, Junbin SHAO
  • Publication number: 20200407775
    Abstract: The present disclosure provides methods and systems for generating and processing optically tagged beads. A nucleic acid probe comprising a fluorescent tag may be hybridized to a bead to generate an optically tagged bead. The nucleic acid probe hybridized to the optically tagged bead may be detectable by one or more signals and may be substantially unreactive to one or more chemical reactions performed with, or in the presence of, the optically tagged bead.
    Type: Application
    Filed: March 10, 2020
    Publication date: December 31, 2020
    Inventors: Rajiv Bharadwaj, Zachary Bent
  • Publication number: 20200407776
    Abstract: This invention pertains to compositions and methods for detecting single and/or multiple nucleotide mismatches on DNA fragments in vitro by enzymatic cleavage using a mixture of mismatch endonucleases. Additionally, this invention pertains to the ability to detect successful genome editing events by programmable nucleases, e.g., TALENS, RGENs, or ZFNs.
    Type: Application
    Filed: June 25, 2020
    Publication date: December 31, 2020
    Inventors: Christopher A Vakulskas, Michael A Collingwood, Mark A Behlke