Abstract: There is disclosed an antifoam component which includes at least one acrylamide polymer for use in a diesel fuel. Acrylamide polymers prepared by polymerizing a (meth)acrylamide monomer to yield a homopolymer or, alternatively, the acrylamide polymer may be prepared by polymerizing a (meth)acrylamide monomer and a (meth)acrylate monomer to yield a heteropolymer.

Abstract: A process and method for removing hydrogen sulfide from a gas and regenerating ferric ions consumed in the hydrogen sulfide scrubbing process at low pH. A two-scrubber regenerative chemical scrubbing system for removing hydrogen sulfide from a gas that provides an economical system for removing hydrogen sulfide from a gas at low pH without the need for chelating agents. An oxide of manganese is used as a catalyst to enhance the regeneration of ferric ions in an aqueous solution under acidic conditions in the presence of oxygen. The process may further include contacting the aqueous solution with a second gas comprising air to replenish the dissolved oxygen in the aqueous solution. The regenerated solution comprising ferric ions can be reused to treat additional hydrogen sulfide containing gases.

Abstract: The invention describes antimicrobial treatment of animal feed, and other matrices with pulsed electric field (PEF) technology or e-beam technology combined with at least one antimicrobial and/or at least one surfactant. The use of this combined methodology approach results in a synergistic reduction of microbial load in the matrix of interest and shows bactericidal effects instead of bacteriostatic effects compared to the use of the technology alone. The addition of an antimicrobial agent in combination with the technology results in a long-lasting antimicrobial effect, preventing re-contamination, which cannot be achieved by using an energetic field alone. Furthermore, the invention describes treatment of the animal feed and other matrices with PEF or e-beam to increase nutrient digestibly of the matrix. Another aspect of the invention relates to providing a suitable alternative to heat treatment, or formaldehyde treatment, in order to decontaminate feed, human and pet food.

Abstract: A liquid anti-friction composition includes an ester product having a number average molecular weight that is greater than 3800 g/mol, and obtained by subjecting a mixture that includes diglycerol, a monobasic acid component, and a dibasic acid component to an esterification reaction. The monobasic acid component includes at least one C14-C24 branched chain fatty acid.

Abstract: The present invention addresses a problem of providing a grease composition that uses hydrophilic nanofibers but still has excellent water resistance and does not readily experience oil separation. The grease composition contains a base oil, hydrophilic nanofibers having a thickness (d) of 1 to 500 nm, and an organic bentonite.

Abstract: A heat-treatment oil composition can make it possible to achieve both high quenching hardness and reduced quenching distortion. A heat-treatment oil composition may include a base oil (A) and a vapor film rupturing agent (B). The heat-treatment oil composition may make it such that, on a cooling curve obtained in accordance with the cooling performance test method in JIS K2242:2012, the time in seconds to 300° C., e.g., the cooling time from 800 to 300° C., is less than 6 seconds. Component (B) may include a petroleum resin.

Abstract: A dispersant for a lubricating oil, containing at least one compound selected from a specific nitrogen-containing compound which is obtained by using, as raw materials, (A) at least one polyolefin selected from polybutene and polyisobutene, (B) at least one maleic acid compound selected from maleic acid and maleic anhydride, and (C) polyamine; a boride of the specific nitrogen-containing compound; and an acylated product of the specific nitrogen-containing compound. The polyolefin (A) satisfies at least one of the following conditions (?) and (?). (?) a ratio (Sb/Sa) of an integrated value (Sb) of a peak present at 4.40 to 5.00 ppm in a 1H-NMR spectrum to an integrated value (Sa) of a peak present at 5.01 to 5.60 ppm in the 1H-NMR spectrum is 2 or more. (?) a ratio (Sd/Sc) of an integrated value (Sd) of a peak present at 1.76 to 2.10 ppm in the 1H-NMR spectrum to an integrated value (Sc) of a peak present at 1.65 to 1.75 ppm in the 1H-NMR spectrum is 1 or more.

Abstract: A lubricant composition includes a base oil, a phenothiazine-based polymerization inhibitor, and a polymerization inhibitory accelerator including a hindered phenol-based compound and a hydroquinone-based compound. A self-polymerization in a polymerizing process is suppressed by an interaction between the polymerization inhibitor and the polymerization inhibitory accelerator. A method of preparing a copolymer using the lubricant composition is also provided.

Abstract: The present disclosure relates to methods of lubricating an electric or a hybrid-electric transmission using a lubricant including a solvent system with a blend of one or more base oils with a branched diester and one or more poly(meth)acrylate copolymers, transmissions therefor, and lubricating compositions suitable for such applications that exhibit good lubricant properties, good electrical properties, and good cooling efficiency at the same time.

Abstract: The present disclosure relates to a process for converting atranol and/or its derivatives into hydrosoluble compound(s). In particular, the present disclosure relates to a process for converting atranol and/or its derivative(s), said process comprising a step of mixing a composition comprising atranol and/or its derivative(s) with an enzyme belonging to the peroxidase family and a peroxide. The present disclosure also relates to a process for producing an oakmoss extract having less than 100 ppm of atranol and/or its derivative(s).

Abstract: Novel methods and compositions for treating textiles and other materials are disclosed in which persistent odor or other symptoms of biofilm presence can be reduced through the use of compositions comprising a biofilm attack agent such as N-acetyl cysteine or certain enzymes, coupled with surfactants and other agents.

Abstract: An aqueous cleaning formulation is provided including a water; an essential oil, wherein the essential oil is selected from the group consisting of lavender oil, rosemary oil, thyme oil and mixtures thereof; a derivative of castor oil having a formula selected from formula I and formula II; an ethoxylated phenol having formula III; and a cleaning surfactant.

Abstract: Detergent compositions and methods of preparing and using the detergent compositions are disclosed. The detergent compositions comprise a polymer system comprising at least one polycarboxylic acid polymer, copolymer, or terpolymer, an alkalinity source comprising an alkali metal carbonate, a nonionic surfactant and water. The detergent compositions are suitable at alkaline pH and reduce or prevent scale formation, improve soil dispersion, and provide effective detergency.

Abstract: Alkaline sprayable aqueous compositions for cleaning, sanitizing and disinfecting are disclosed. In particular, the sprayable compositions include an alkali soluble emulsion polymer, an alkalinity source, a foaming agent, and water. Beneficially, the sprayable cleaning compositions have reduced misting and are environmentally safe for users to apply. Further the compositions exhibit less running on non-horizontal surfaces than acrylamide-based compositions.

Abstract: The invention provides a process for the preparation of a modified triethanolamine/fatty acid ester composition ratio by treatment of a conventional tnethanolamine fatty acid ester composition with a hydrolytic enzyme. Also provided are novel mixtures of tnethanolamine and mono-, di-, and tri-esters of fatty acids which are useful in the preparation of cationic surfactants useful in fabric softening applications. In one aspect, the method increases the triester fraction without significantly affecting the amount of mono-ester and unesterified species, which in turn adds to the flexibility in the formulation of the corresponding fabric softening compositions.

Abstract: Disclosed is the use of polypeptides having carbohydrate binding properties, such as CBM, for reducing the wrinkles in laundry. Also detergent compositions comprising CBM are disclosed.

Abstract: The present invention is directed to a cleaning composition for sanitizing or disinfecting hard surfaces containing a cationic biocide, surfactant and low levels of VOC solvents. The cleaning composition is adapted to clean a variety of hard surfaces without leaving behind a visible residue and creates low levels of streaking and filming on the treated surface. The cleaning composition contains less than 5% by weight of VOCs. The cleaning composition may be used alone as a liquid or spray formulation or in combination with a substrate, for example, a pre-loaded cleaning wipe.

Abstract: The present invention relates to a process for preparing a spray-dried laundry detergent particle, wherein the process includes the step of contacting water-insoluble silicate salt to monomeric organic carboxylic acid in an aqueous mixture, wherein the aqueous mixture has a pH of 4.2 or less, wherein the aqueous mixture includes detersive surfactant, wherein the aqueous mixture is substantially free of carbonate salt, wherein the water-insoluble silicate salt reacts with the monomeric organic carboxylic acid to form silica, wherein the aqueous mixture is spray-dried to form a spray-dried laundry detergent particle, wherein the particle comprises: detersive surfactant; monomeric organic carboxylic acid; and silica, wherein the particle is substantially free of carbonate salt.

Abstract: The purpose of the present invention is to provide a technique whereby multiple projection targets are efficiently identified from multiple neurons in multiple brain areas with the use of multis-point light stimulation. An acquisition unit 52 acquires spike signals generated from multiple neurons existing in the vicinity of two or more recording sites. A stimulation control unit 51 selects one projection target candidate from two or more candidates in accordance with a definite system on the basis of the spike signals and then determines irradiation timing of light stimulation. Upon the light stimulation, a management unit 53 acquires the spike signals in all of the recording sites within a definite period of time before or after the light stimulation, while dividing the spike signals into anti responses and collision responses. An anti response management unit 81 acquires and manages information relating to the anti responses.

Abstract: The present disclosure is drawn to microfluidic cellular membrane modification devices. In one example, a microfluidic cellular membrane modification device can include a microfluidic channel including a pumping portion and an electric field portion. An electrode pair can be positioned about the electric field portion. A bidirectional pump can be in fluid communication with the microfluidic channel at the pumping portion to move fluid backward and forward through the electric field portion.

Abstract: The subject invention concerns materials and methods for culture of cell aggregates. The subject invention utilizes three-dimensional (3-D) inserts comprising micro-channels having selected dimensions. The inserts are provided in or on tissue culture plates that can be supported on a programmable rocking platform/station, thereby providing for a hydrodynamic environment that promotes 3-D aggregation of cells cultured on the plates. The supporting rocker is programmed to provide motion that generates hydrodynamic conditions that support 3-D cell aggregation and long-term culture. The subject invention also concerns an apparatus comprising a tissue culture vessel that comprises a 3-D insert of the present invention, and a programmable rocking platform/station that can provide motion to the vessel provided thereon, thereby generating hydrodynamic conditions and wave motion that support 3-D cell aggregation and cell culture. The subject invention also concerns methods for growing 3-D aggregates of cells.

Abstract: A sampler for use with a bioreactor for growing a cell culture is disclosed. In one embodiment, the bioreactor includes a structured fixed bed including a removable sample portion for recovering a sample of cells from the cell culture. Related apparatuses and methods are also disclosed.

Abstract: When detachment of a cell sheet from a culture dish has started at an unintended timing, immediately detach the cell sheet from the culture dish while preventing generation of wrinkles and the like in the cell sheet. A cell culture device captures an image of cells in the culture dish, and controls a cooling mechanism and a shaking mechanism based on the image so as to shake the cells while cooling them.

Abstract: An object of the invention is to provide a cell culture monitoring device capable of monitoring the number of cells during culture and a cell culture system. The cell culture monitoring device for monitoring proliferation of cells includes: a detection unit configured to detect particles of exosomes in culture supernatant; and an analysis unit configured to calculate the number of cells based on an obtained detection result. The cell culture system includes the cell culture monitoring device and an automatic culture device.

Abstract: A fluid pumping and bioreactor system including at least two cassettes, at least one storage reservoir, at least one bioreactor, at least one manifold including valve modules, and tubing to connect the cassettes to the storage reservoir and the bioreactor. The cassettes can include pumps, valves, and fluid conduits and can be communicatively connected to the at least one manifold. The bioreactor can include an adapter and fluid conduits extending through the adapter from the exterior of the bioreactor to the interior of the bioreactor. System and method for generating a tissue for transplant by decellularizing and recellularizing a supplied tissue.

Abstract: Disclosed herein is an improved system and method for transporting biological samples, including a unique transport medium configured to protect bacterial viability and optimize sample quality which greatly enhances diagnostic accuracy. The improved system and methods can include a transport media, improved collection tube including a screen and sampling brush, and an improved transport case. The improved systems and methods allows samples to be frozen and thawed while preserving cellular wall integrity during transport or storage, and furthermore can preserve the survival of the bacterial sample, which dramatically increases the ability of the lab to correctly identify the infectious agent. The improved systems and methods allow for samples to be incubated at 37 degrees Celsius for up to 24 hours in order to increase the density of bacteria in samples with low initial bacterial density at collection.

Abstract: The present invention relates to a method for restoring sexual reproduction between two sterile, female strains of Trichoderma reesei using a helper strain ?MAT, said helper strain being a fertile female strain of Trichoderma reesei in which the sexual-type locus MAT has been eliminated.

Abstract: Disclosed herein is a low-cost method to maximize malonyl-CoA production in E. coli, and consequently a high yield of its derived bioproducts.

Abstract: The present invention provides Streptomyces coelicolor strain A3(2)_M22-2C43 obtained by inducing a point mutation in the base sequence of the DagB gene in a wild-type Streptomyces coelicolor strain A3(2) by UV radiation. Since the Streptomyces coelicolor strain A3(2)_M22-2C43 according to the present invention expresses a DagB mutant enzyme expressing little or no DagB beta-agarase or exhibiting little or no beta-agarase activity, there is no need for separate isolation and purification of DagA enzymes from culture fluid, and the culture fluid of the Streptomyces coelicolor strain A3(2)_M22-2C43 or supernatant thereof may be used to produce, from agar or agarose, neoagarose oligosaccharides with a higher content of neoagarotetraose or neoagarohexaose than that of neoagarobiose.

Abstract: The present invention provides a composition for promoting cell proliferation containing polylysine in which at least one amino group is mono-substituted by dicarboxylic acid.

Abstract: The present invention is directed generally to dry cell culture media or feeds in pellet formats which can be reconstituted into liquid media for culturing cells in vitro. Each pellet composition may comprise the same or a different composition; for example, different vitamins, amino acids, buffers, trace salts, pH, iron chelators, etc. The invention also relates to methods of making dry cell culture media by altering ratios of different pellet compositions, or, methods of making modular dry cell culture media, or customizing media formulations for growing a cell type using pellets. The media pellets may be easier to handle either before reconstitution, during shipping and handling; and/or during reconstitution. Media pellets may be used in any container like bags including sterile, single use bags for preparing media formulations. The invention also relates to kits and culture systems using media pellets.

Abstract: By establishing effective methods for shrimp 3D cell culture and passage, the present invention provides a technology of continuous shrimp cell culture intended for the establishment of immortalized shrimp cell lines. The present invention provides a preparation method of matrigel for 3D cell culture of shrimp by optimizing an additive proportion of matrigel. The present invention further provides a technology of separation and 3D cell culture of shrimp haemolymph cells, where shrimp haemolymph cells adhere to and grow on the surface of the matrigel in the form of a single round cell and a cell pellet/cellular spheroid, with survival and growth abilities being superior to 2D culture effects. The above technology is achieved by optimizing a formula of complete medium for shrimp cells, selecting the medium as an anticoagulant and a diluent for shrimp haemolymph cells, selecting a 3D culture method for surface-adhered growth in the matrigel.

Abstract: The present invention relates to a method for isolating umbilical cord-derived stem cells, and more specifically to a method for isolating a significantly large number of mesenchymal stem cells from the umbilical cord having a specified size. The method of the present invention has a great advantage in that since stem cells can be isolated from an umbilical cord tissue without enzymatic treatment, stress applied to the cells can be significantly suppressed. In addition, stem cells obtained by the method of the present invention have superior proliferative capacity compared to stem cells obtained by conventional isolation methods.

Abstract: Methods are provided for generating multiple cellular products via differentiation of cells from single clinically compliant pluripotent cells into multiple cellular products selected from retinal epithelium, retinal progenitors, neural stem cells, dopaminergic neurons, astrocytes, hepatocytes, endothelial cells and mesenchymal cells using standard differentiation protocols for the multiple cellular products.

Abstract: The present invention relates to a method of screening highly efficiently stem cells using the protein marker GRP78, and more particularly, to a method of removing old stem cells with decreased expression of GRP78 and isolating only highly efficient stem cells. The use of the protein marker GRP78 according to the present invention makes it possible to isolate only highly efficient stem cells by removing old stem cells. Thus, the protein marker GRP78 may be used as an effective marker for controlling the quality of stem cell therapy products and evaluating the stability and effectiveness thereof, and is useful in the production of stem cell therapy products having excellent therapeutic efficacy.

Abstract: Culture medium formulations are described that are capable of large-scale expansion of stem cell-derived hepatocyte-like cells while sustaining the activity of those cells to maintain the phenotype and biological characteristics of normal hepatocytes.

Abstract: An exosome active formulation for promoting endothelial cell angiogenesis, and a preparation method and use thereof include the following steps: isolating primary umbilical vein endothelial cells and performing cell culture and passage; adding anisodamine to a culture medium of the subcultured endothelial cells to treat the endothelial cells, and then replacing the culture medium with a new endothelial cell culture medium to continue the culture of the endothelial cells; extracting exosomes from the endothelial cell culture medium obtained after the endothelial cells are cultivated; and identifying the exosomes. The exosome active formulation for promoting endothelial cell angiogenesis prepared by the preparation method and its use in the manufacture of a medicament for treating microvascular injury, microcirculation dysfunction and cardio-cerebral infarction.

Abstract: BANF1, PPP2CA, and ANKLE2 were identified as genes that promote tau aggregation when disrupted. Improved tauopathy models such as cells, tissues, or animals having mutations in or inhibition of expression of BANF1 and/or PPP2CA and/or ANKLE2 are provided. Methods of using such improved tauopathy models for assessing therapeutic candidates for the treatment of a tauopathy, methods of making the improved tauopathy models, and methods of accelerating or exacerbating tau aggregation in a tauopathy model are also provided.

Abstract: The present invention relates to an angiogenesis-inducing method using neural stem cells. According to the present invention, neural stem cells derived from human brain tissues release MCP-1 or Gro, thus exhibiting an angiogenesis-inducing effect. When CoCl2 was treated to the neural stem cells of the present invention and when the neural stem cells were cultured in a hypoxia chamber, it was observed that a hypoxia condition increased the release amounts of MCP-1 and Gro. Therefore, the human brain tissue-derived neural stem cells of the present invention can be used to induce angiogenesis.

Abstract: A population of immune cells comprising modified immune cells with reduced inflammatory properties, wherein such modified immune cells may have reduced production of one or more inflammatory cytokines (e.g., interleukin 2) and/or express one or more antagonists of one or more inflammatory cytokines (e.g., interleukin 6). Also provided herein are methods of producing such immune cell populations comprising the modified immune cells and methods of using such in cell therapy (e.g., to treat cancer, infectious diseases or immune diseases).

Abstract: The present invention relates to a cancer-specific tumor antigen neoepitope represented by any one of SEQ ID NOs: 1 to 184, an antigen-presenting cell loaded with the neoepitope, and a method for activating T cells for cancer treatment using the antigen-presenting cell. An antigen-presenting cell, that is, a dendritic cell, loaded with a cancer-specific tumor antigen epitope provided in the present invention enables rapid and effective induction of differentiation and proliferation of cancer antigen-specific T cells, preferably memory T cells, and the memory T cells thus activated can treat a cancerous or neoplastic condition or prevent recurrence, progression, or metastasis of cancer while avoiding the defense mechanism of cancer cells.

Abstract: Cord blood or peripheral blood NK cells are prepared from whole blood mononuclear cells without the need to isolate CD34+ hematopoietic stem cells or NK cells, and without the need for a feeder layer. Advantageously, the methods presented herein use an enrichment process that uses antiCD16 agonist antibodies, antiCD3 antibodies, and N-803. Moreover, contemplated processes are suitable for adaptation into a fully automated production process (GMP in a box).

Abstract: Cord blood or peripheral blood NK cells are prepared from whole blood mononuclear cells without the need to isolate CD34+ hematopoietic stem cells or NK cells, and without the need for a feeder layer. Advantageously, the methods presented herein use an enrichment process that uses antiCD16 agonist antibodies, antiCD3 antibodies, and N-803. Moreover, contemplated processes are suitable for adaptation into a fully automated production process (GMP in a box).

Abstract: The present invention relates in part to nucleic acids encoding proteins, nucleic acids containing non-canonical nucleotides, therapeutics comprising nucleic acids, methods, kits, and devices for inducing cells to express proteins, methods, kits, and devices for transfecting, gene editing, and reprogramming cells, and cells, organisms, and therapeutics produced using these methods, kits, and devices. Methods for inducing cells to express proteins and for reprogramming and gene-editing cells using RNA are disclosed. Methods for producing cells from patient samples, cells produced using these methods, and therapeutics comprising cells produced using these methods are also disclosed.

Abstract: The present invention provides a cardiomyocyte maturation promoter. The present invention provides a cardiomyocyte maturation promoter comprising one or more compounds selected from 2-methoxy-5-((Z)-2-(3,4,5-trimethoxyphenyl)vinyl)phenol, (1-ethyl-1H-benzotriazol-5-yl)methyl (2-(2-methoxy-4-methylphenyl)-4-methyl-1,3-thiazol-5-yl)carbamate, (2?beta)-22-oxovincaleukoblastine, 2-(2-(4-chlorophenyl)ethyl)-6-(2-furyl)-3H-imidazo[4,5-b]pyridine, 4,5-anhydro-1,2-dideoxy-4-methyl-2-((N-(morpholin-4-ylacetyl)-L-alanyl-O-methyl-L-tyrosyl)amino)-1-phenyl-L-threo-pent-3-ulose, 3-(3-methoxyphenyl)-N7,N7-dimethylisoquinoline-1,7-diamine, methyl 4-(2-benzylbenzoyl)-2,5-dimethyl-1H-pyrrole-3-carboxylate, 2?-(4-aminophenyl)-1H,1?H-2,5?-bibenzimidazol-5-amine, and salts thereof.

Abstract: Provided are a cardiomyocyte preparation, a preparation method therefor and the use of the preparation in treating heart failure. The cardiomyocyte preparation comprises cardiomyocytes and fibroblasts, differentiated from pluripotent stem cells, wherein the concentration of the cardiomyocytes in the cardiomyocyte preparation is 0.75×107-1.0×109 cells/mL, and the content of the cardiomyocytes is higher than 80% while the content of the fibroblasts is not higher than 20%. The cardiomyocyte preparation can improve cardiac ejection fraction and left ventricular fractional shortening, and increase the thickness of the left ventricular inner wall.

Abstract: The present disclosure relates to a novel musculoskeletal stem cell (MSSC) differentiated from an ESC (embryonic stem cell) or an iPSC (induced pluripotent stem cell). The musculoskeletal stem cell of the present disclosure can be easily induced from a human embryonic stem cell or a human-derived pluripotent stem cell and can be effectively differentiated not only into bone but also into cartilage, tendon and muscle. Accordingly, it can be usefully used for prevention or treatment of various musculoskeletal diseases.

Abstract: A method of enhancing osteogenic differentiation using vitamin D treatment is disclosed. The method utilizes the combined effect of vitamin D treatment and flow-induced shear stress in a modified perfusion bioreactor to treat the bone defect. The method comprising the steps of: isolating adipose tissue from a subject by liposuction; separating adipose-derived stem cells from the adipose tissue; pre-treating the separated adipose-derived stem cells for a predefined time 20 to 40 minutes with vitamin D3; seeding the pre-treated stem cells onto one or more scaffolds; washing of unattached stem cells from the scaffolds after a predefined time of 20 to 30 minutes; culturing the stem cell seeded scaffold by utilizing a modified perfusion bioreactor to form a tissue-engineered construct, where flow induced shear stress is applied, and implanting the tissue-engineered construct into the subject without a need to obtain autologous bone graft.

Abstract: An object of the present application is to provide a method for inducing the differentiation from pluripotent stem cells, particularly iPS cells and ES cells, into ureteric bud cells. Another object of the present application is to provide a system for producing ureteric bud-like tissue from pluripotent stem cells through each stage of differentiations, i.e. anterior primitive streak cells, anterior intermediate mesoderm cells and Wolffian duct cells. Yet another object of the present application is to provide a method for maintaining ureteric bud-like organoids. Another object of the present application is to provide a method for expansion-culturing ureteric bud-like tip tissue.

Abstract: Methods and materials are described for producing immortalized hybrid cells composed of a fusion of immortalized multi-lineage progenitor cells (MLPC) and primary hepatocytes. The hybrid cells express the biological activity of the primary hepatocytes and the immortality and expansion capacities of the immortalized MLPC. The methods of culture and expansion of the resultant hybrid cells and the methods to confirm the characteristics of the hybrid cells are described.