Abstract: The microfluidic platform device of the claimed invention makes use of a high throughput lab-on-a-chip format to determine the functional profile of antibodies elicited by infection or vaccination against infection for any pathogen. It can be used to evaluate vaccines, evaluate whether vaccinated individuals show indices of protection, determine whether individuals display resistance or susceptibility to infection, discover new vaccine antigens, discover and test therapeutic interventions, or evaluate mechanisms of disease.

Abstract: The invention relates to a bioassay of insulin peptide in oral formulations and low affinity insulin peptides in liquid formulations, by for quantifying phosphorylated Akt, thereby avoiding the interference of excipients in potency determination.

Abstract: The present application provides a method of analyzing protein binding sequence, a method of making sequencing library, and compositions for performing such methods, which employs a transposome complex.

Abstract: The present invention provides assays and assay systems for use in spatially encoded biological assays. The invention provides an assay system comprising an assay capable of high levels of multiplexing where reagents are provided to a biological sample in defined spatial patterns; instrumentation capable of controlled delivery of reagents according to the spatial patterns; and a decoding scheme providing a readout that is digital in nature.

Abstract: Methods and kits for accurately detecting one or more analytes in a sample by removing non-specific binding signals utilizing capture and control microparticles. The capture microparticles can specifically bind to the analyte while the control microparticles do not specifically bind to the analyte but to the background molecules. Both capture and control microparticles are added to the sample under suitable conditions to allow binding between analytes and the microparticles. Detection agent is then added to bind to analytes and other substances captured by the microparticles. The microparticles are then run through a cytometry-based detection method, where detection signals from the capture and the control microparticles are distinguished.

Abstract: The invention relates to a method for quantitatively determining biological analytes in an aqueous solution in the presence of one or more functionalised surfaces, wherein the aqueous solution comprises at least one type of biological analyte and at least one type of fluorescence marker, characterised in that the quantity and/or concentration of the biological analyte or analytes is determined by measuring the fluorescence emission of the unbound fluorescence markers, as well as to a devices for carrying out said method.

Abstract: Provided herein are compositions and methods for determining the structure of individual targets using by determining long-range distances within such targets.

Abstract: Compositions which comprise a gold nanoparticle, dithiolated diethylenetriamine pentaacetic acid (DTDTPA), and a thioctic acid terminated peptide, wherein the DTDTPA is directly linked to the gold nanoparticle surface via an Au—S bond, and wherein the thioctic acid terminated peptide is directly linked to the gold nanoparticle surface via an Au—S bond.

Abstract: Accordingly, in some embodiments methods for detecting an analyte or analytes in one or more sample(s) are provided. The methods encompass providing a solid support with an addressable marker and an associated ligand, contacting the solid substrate to a sample, thereby forming a contacted solid support, associating the contacted solid support with a FET array and detecting the electrical properties of the FET array and thereby detecting an analyte or analytes in one or more samples. In other embodiments, the sample encompasses a second addressable marker.

Abstract: The present invention relates to a nanovesicle comprising a heterodimeric G-protein coupled receptor, a method for preparing the nanovesicle, a field effect transistor-based taste sensor comprising the nanovesicle, and a method for manufacturing the taste sensor. The field effect transistor based taste sensor functionalized by the nanovesicle comprising the heterodimer G-protein coupled receptor according to the present invention has excellent sensitivity and selectivity and may highly specifically detect a sweet taste substance in real time, by using the heterodimeric G-protein coupled receptor and the nanovesicle comprising the same.

Abstract: The invention relates to a device and a method for automated evaluation of incubated immunoblot strips (5). What is essential for such immunoblot strips (5) is that these have regions coated with antigens, which regions discolour under the formation of so-called bands (6) as soon as a binding occurs, in particular between the antigen and an antibody present in a patient sample. Within the scope of the described technical solution, a camera (2) is initially used to make recordings of the surface of an incubated immunoblot strip (5) and these recordings are transmitted in digitized form to an evaluation unit (1). Finally, the discolourings, which occurred in the form of bands (6), are detected and at least partly quantified in the evaluation unit (1), such that a diagnosis suggestion is generated taking into account the number of bands (6) and the colour strength thereof.

Abstract: A subtractive immunoassay for detecting the presence or absence of first and second analytes in a sample includes in the following order the steps of: a) conjugating a first detector antibody to the first analyte to tag the first analyte with a first detector tag; b) conjugating a first capture antibody to the first analyte to capture the first analyte to completely deplete the first analyte from the sample; c) conjugating a second detector antibody to the second analyte in the depleted sample to tag the second analyte with a second detector tag; d) conjugating a second capture antibody to the second analyte to capture the second analyte from the sample; e) detecting presence or absence of the first and second detector tags at the site of the first and second capture antibody, respectively, and thereby detecting the presence or absence of the first and second analytes in the sample.

Abstract: Mycobacterial-specific biomarkers and methods of using such biomarkers for diagnosis of mycobacterial infection in a mammal are disclosed.

Abstract: A method of detecting serous ovarian cancer, particularly early stage serous ovarian cancer is described. The method uses up to sixteen compounds found in the blood serum of a person, and determines that changes of some portion of these sixteen compounds can determine the presence of serous ovarian cancer in a women with up to 100% accuracy, specificity, and sensitivity.

Abstract: Disclosed are 13C and 15N derivatization reagents and their use for gas chromatography-mass spectroscopy and liquid chromatography/mass spectroscopy chemical identification and quantification.

Abstract: Methods for producing isotopically-labelled peptides are provided. Aspects of the method include: contacting a sample including a metabolically tagged protein with a cleavable probe to produce a probe-protein conjugate; separating the probe-protein conjugate from the sample; digesting the probe-protein conjugate to produce a probe-peptide conjugate; and cleaving a cleavable linker to release an isotopically labelled peptide. The method may further include: identifying a predetermined isotopic pattern in a mass spectrum; determining an amino acid sequence of the isotopically labelled peptide; and identifying the site of protein glycosylation based on the determined amino acid sequence. Also provided are cleavable probes for practicing the subject methods, described by the Formula: A-L-(M-Z) where A is an affinity tag, L is a cleavable linker, M is an isotopic label and Z is a chemoselective tag capable of cross-linking a metabolically tagged protein.

Abstract: Provided are methods and kits, e.g.; for testing the immune status of patients with chronic inflammatory diseases by measuring the TCR zeta chain (CD247) expression levels, and in particular a method and a kit for testing the selective downregulation of TCR zeta chain expression in T cells, NK cells, or NKT cells. Zeta chain expression is measured using antibodies directed against the intracellular zeta chain region, and these levels are compared with the expression levels of other T cell receptor subunits and NK cell markers. Provided are methods for determining diabetes complications in a diabetic patient. Further provided are kits for diagnosis, prognosis, and monitoring of same.

Abstract: Disclosed is a method for diagnosing allergy in a human or animal patient, wherein the level of species-specific lipocalin, especially lipocalin 2 in a human patient, is measured in a sample of said patient and wherein a lowered level of said lipocalin compared to the level of said lipocalin in the corresponding sample of a human or animal that has no allergy, is indicative of an allergy, as well as a kit for performing this method, and a kit for quality control of allergen molecules or extracts used for immunotherapy of allergy by determining its siderophore-iron ligand load and thus immunomodulatory potency. Further, lipocalin proteins for use in treatment or prevention of allergies are provided, preferably complexed with siderophore-iron ligands.

Abstract: The present invention provides biomarker-based methods for diagnosing stroke in a patient suspected of having suffered a stroke, and also for discriminating between ischemic stroke and transient ischemic attack. Substrates comprising probes for specific combinations of biomarkers useful in the methods of the invention are also described.

Abstract: The invention relates to the field of the diagnosis and prognosis methods of molecular pathologies. In particular, the invention relates to methods for determining the diagnosis of an ectatic disease of the cornea in a subject, for determining the risk of developing an ectatic disease of the cornea in a subject, for determining the clinical outcome of a subject suffering from an ectatic disease of the cornea and for selecting a subject to be treated with a therapy for an ectatic disease of the cornea based on the determination of the expression levels of TLR2 and/or TLR4 markers. The invention also relates to the use of the TLR2 and/or TLR4 as diagnosis and prognosis markers for an ectatic disease of the cornea.

Abstract: The present disclosure provides immunoassay methods of detecting a cleaved high molecular weight kininogen (HMWK) with high sensitivity and specificity and isolated antibodies that specifically bind cleaved HMWK.

Abstract: The present description relates to methods for clinically assessing Parkinson's disease in a subject using erythrocyte-derived extracellular vesicles (EEV) as a biomarker.

Abstract: The present invention relates to the field of cognitive function. More specifically, the present invention provides compositions and methods useful for assessing cognitive dysfunction/function in Alzheimer's disease and other diseases of cognition. In one embodiment, the method comprises the steps of (a) reducing heterocomplexes comprising NPTX1 and NPTX2 present in a biological sample obtained from the patient into NPTX1 and NPTX2 monomers; (b) covalently modifying the thiol groups of the NPTX1 and NPTX2 monomers to prevent re-formation of NPTX1/NPTX2 heterocomplexes; (c) detecting NPTX2 in the sample; and (d) assessing cognitive function in the patient by comparing NPTX2 detected in the sample to a control.

Abstract: A center processor generates multiple instruction strings from sequence data based on a conversion table. The multiple instruction strings are transmitted to multiple local units via an internal network. Each of the local units includes a local processor and an I/O board. Layers from an internal layer to an input/output layer form a common platform.

Abstract: A sample is measured using a cartridge from a batch comprising a plurality of cartridges of the same type. The measurement results measured in the process are evaluated. In order to evaluate the measurement results, reference results are used in addition, which reference results were measured previously, separately, during measurements of reference samples, using a plurality of cartridges of the same batch. An analyte of the sample is determined from the measurement results. During the evaluation, the reference results and/or measurement results are preferably normalized.

Abstract: This sample rack conveying apparatus is provided with a pusher unit, a linear motion guide, and a conveyance drive mechanism. The pusher unit has a base portion, a moving member, and a base-side guide. The base portion is supported by the linear motion guide so as to be movable in a first guide direction. The moving member is provided with a pusher that pushes a sample rack. The base-side guide supports the moving member such that the moving member is movable in a second guide direction that crosses the first guide direction.

Abstract: A system senses, analyzes, and reports a collision event experienced by a person or object. A sensor module records angular velocities over time and a processor analyzes the sensed velocities, calculates properties of angular velocities, such as jerk and jolt, compares these properties with threshold values selected to correlate to predicted severities of injury to the person or object, and transmits information regarding these properties to a communication device controlled by user-designated persons. Group tracking and communication devices ar used by monitors to manage multiple persons equipped with sensor modules. The sensor modules and group tracking and communication devices are designed to be portable, attachable, and detachable so that they can be attached to different types of gear used by persons engaging in different activities.

Abstract: Methods and apparatus for obtaining extremely high sensitivity chemical composition maps with spatial resolution down to a few nanometers. In some embodiments these chemical composition maps are created using a combination of three techniques: (1) Illuminating the sample with IR radiation than is tuned to an absorption band in the sample; and (2) Optimizing a mechanical coupling efficiency that is tuned to a specific target material; (3) Optimizing a resonant detection that is tuned to a specific target material. With the combination of these steps it is possible to obtain (1) Chemical composition maps based on unique IR absorption; (2) spatial resolution that is enhanced by extremely short-range tip-sample interactions; and (3) resonant amplification tuned to a specific target material. In other embodiments it is possible to take advantage of any two of these steps and still achieve a substantial improvement in spatial resolution and/or sensitivity.

Abstract: A miniature probe for measuring small voltage signals of a DUT includes a probe body having a flexible substrate and signal transmission lines running a longitudinally, and a first probe connection circuit located at a first end of the probe body and including exposed wires, SMT components coupled between the exposed wires and the signal transmission lines, respectively, and a local mechanical stiffener mounted adjacent the SMT components. The wires are connectable to the DUT for receiving the voltage signals. The probe further includes a second probe connection circuit located at a second end of the probe body, and including transmission line connectors coupled to the signal transmission lines, respectively, and a bent portion of the flexible substrate between the probe body and the transmission line connectors. The bent portion enables the transmission line connectors to exit the probe substantially axially, relative to the longitudinal length of the probe body.

Abstract: An example apparatus includes a connection module. The example connection module includes a connection interface and a connection matrix having a root transmission line to conduct signals to and from the connection interface. The connection matrix also includes branch transmission lines that are connectable electrically to the root transmission line to conduct the signals to and from the root transmission line. Each of the branch transmission lines is part of an electrical pathway between a device and the root transmission line. A housing encloses the connection matrix and enables access to the connection interface. The root transmission line and the branch transmission lines are each multi-conductor transmission lines that conduct the signals in transverse electromagnetic (TEM) mode.

Abstract: This inspection jig is provided with: an inspection-side support member having a counter plate (51) provided with a facing surface (F) disposed to face the substrate; and an electrode-side support member (6) having supporting plates (61-63) disposed to face an electrode plate (9) located on the side opposite to the facing surface (F) of the counter plate (51) A probe supporting hole (23), into and by which the rear end portion of the probe (Pr) is inserted and supported, is provided in the supporting plates (61-63), and the probe supporting hole (23) is provided with a restricting surface which is formed along a supporting line (V) inclined at a certain angle (?) with respect to a reference line (Z), and which restricts the rear end portion of the probe (Pr) from moving in the direction perpendicular to the inclined direction of the supporting line (V).

Abstract: The invention relates to a method for placing and contacting a contact element formed in particular as a test contact of a test contact arrangement, wherein in said method, for forming a heat transfer surface, a contact head provided with a contact element holding device, with the contact element received in the contact element holding device, is placed between the contact element and a contact material deposit arranged on a contact surface of a contact carrier in the direction of a feeding axis against a contact surface of the connecting material deposit, and, for realizing an at least partial fusing of the connecting material deposit and for producing a materially bonded connection between the contact element and the connecting material deposit, thermal energy is introduced into the connecting material deposit by means of treating the contact element with thermal energy, the temperature T of the contact element being measured while the contact element is being treated and the duration of the treatment being

Abstract: In general, the subject matter described in this disclosure can be embodied in methods, systems, and program products for changing the manner in which an input signal is filtered based on a characteristic of an electronic test instrument. The method includes receiving, by the electronic test instrument, user input that modifies a characteristic of a channel of the electronic test instrument, and as a result changing the electronic test instrument from having the first digital filtering configuration to having a second digital filtering configuration, the first digital filtering configuration specifying that all or a majority of digital filtering occurs after the acquisition memory in the channel and the second digital filtering configuration specifying that all or a majority of digital filtering occurs before the acquisition memory in the channel.

Abstract: A voltage detector includes a voltage division circuit which outputs a divided voltage based on an input voltage, a comparison circuit which compares the divided voltage and a reference voltage to output a detection signal and a release signal, and a voltage limiting circuit which limits the divided voltage to a preset voltage.

Abstract: Methods and systems are described for monitoring and compensating an offset between a reference voltage used in a first device and a corresponding reference voltage used in a second device. The first device can include offset circuitry. The offset circuitry receives two voltage signals. The first voltage signal is equal to a first voltage value that is used as a reference voltage in the first device. The second voltage signal can be a time-varying voltage signal that has a known relationship with a second voltage value that is used as a reference voltage in the second device. The offset circuitry can then determine the second voltage value from the second voltage signal, and output an offset value based on a difference between the first voltage value and the second voltage value.

Abstract: A current transducer including a housing comprising an outer casing, a magnetic circuit core configured to encircle at least one primary conductor, and a magnetic field detector positioned in a magnetic circuit gap of the magnetic circuit core. The housing further includes an inner support part comprising a main body portion and a magnetic core support portion. The magnetic circuit core is in the form of a thin strip of amorphous material supported on the main body portion and magnetic core support portion, the magnetic circuit gap being formed between overlapping ends of the strip.

Abstract: A magnetic balance type current sensor includes a plurality of coils and a magnetic detection element whose characteristics change depending on a magnetic field induced by a current to be measured. Each of the plurality of coils includes a coil wire section disposed on a surface located away from a reference plane, a lead wire section connected to the coil wire section, and two electrode sections connected individually to the coil wire section and to the lead wire section. Each of the plurality of coils is capable of generating a canceling magnetic field when a feedback current is passed by using the electrode sections. The lead wire sections and the coil wire sections have crossing portions in which the lead wire sections and the coil wire sections are layered with an insulating section interposed so as to overlap each other in a winding axis direction of the coil wire sections.

Abstract: A current sensor can include a lead frame. The lead frame can include a first lead and a second lead, wherein the first and second leads are coupled together at a first junction region of the lead frame, wherein the current sensor is operable to sense a magnetic field generated by a first current passing through the first junction region. The current sensor can further include a first die disposed proximate to the lead frame. The first die can include a first magnetic field sensing element disposed on a surface of the first die, a first circuit coupled to the first magnetic field sensing element for generating a first signal indicative of a first current, and a first node coupled to the first signal. The current sensor can further include a second die disposed proximate to the lead frame.

Abstract: A Fast Faraday cup includes a group of electrodes including a ground electrode having a through hole and a collector electrode configured with a blind hole that functions a collector hole. The electrodes are configured to allow a beam (e.g., a non-relativistic beam) to fall onto the ground electrode so that the through hole cuts a beamlet that flies into the collector hole and facilitates measurement of the longitudinal distribution of particle charge density in the beam. The diameters, depths, spacing and alignment of the collector hole and the through hole are controllable to enable the Fast Faraday day cup to operate with a fast response time (e.g., fine time resolution) and capture secondary particles.

Abstract: The present disclosure relates to a measuring arrangement for identifying a malfunction of an energy accumulator arrangement having a first energy accumulator and at least one further energy accumulator, the first energy accumulator and the further energy accumulator being electrically connected in parallel, comprising: a measuring device configured to capture a polarity of an electrical measurement variable of the first energy accumulator and a polarity of a further electrical measurement variable in the energy accumulator arrangement; and a processor device configured to identify the malfunction of the energy accumulator arrangement by comparing the polarity of the electrical measurement variable of the first energy accumulator and the polarity of the further electrical measurement variable.

Abstract: A voltage detector (200) for monitoring an input signal and outputting a detection signal at an output when a voltage of the input signal meets a first threshold having: an input configured for receiving the input signal; a voltage reference circuit for receiving an input voltage and producing a reference voltage having a maximum value independent of the input voltage; and a trigger configured to compare the input signal and the reference voltage and to output a detection signal to the output when the voltage of the input signal reaches the first threshold. The voltage reference circuit comprises a reset input connected to either the input or the output and is configured to reduce the reference voltage when a predetermined reset signal is received.

Abstract: According to an aspect of a present invention, there is provided a semiconductor device including a first power monitoring device and a second power monitoring device. The first power monitoring device outputs first operating power that is to be supplied to a second control section. The second power monitoring device outputs second operating power that is to be supplied to a first control section. Based on a first setting given from the first control section, a first power monitoring circuit autonomously verifies whether the second operating power is normal, and periodically transmits the result of verification to the second control section as first error information. Based on a second setting given from the second control section, a second power monitoring circuit autonomously verifies whether the first operating power is normal, and periodically transmits the result of verification to the first control section as second error information.

Abstract: Various embodiments relate to detecting theft of electrical energy. A method of detecting theft of electrical energy may include measuring, for each time sample of a number of time samples, a neutral current of an electrical energy metering system. The method may further include summing, for each time sample of the number of time samples, a number of measured phase current values of the electrical energy metering system to determine an imputed neutral current. Further, the method may include determining, for each time sample of the number of time samples, a squared difference between the measured neutral current and the imputed neutral current. Moreover, the method may include integrating, for each time sample of the number of time samples, the squared difference to determine an accumulator value. In addition, the method may include detecting, based on the accumulator value, theft of electrical energy from the electrical energy metering system.

Abstract: Conventional real-time spectrum analyzers have a degree of technical complexity in the hardware which increases disproportionately as the analysis bandwidth increases for Fourier transformations of the measured sampling values. When using high analysis bandwidths, a detailed resolution is not needed of each analyzed individual frequency on the time plane at the same time; instead, detection of the presence of short pulses can be important as well. For this application, mixing sampling values on the time plane using a variable auxiliary frequency allows the sampling rate to be reduced, in that the bandwidth is maintained but a compression is carried out on the time plane. A very high time resolution which far exceeds the capabilities of conventional real-time spectrum analyzers can additionally be achieved overall for the analysis bandwidth, the time resolution then being computationally assignable to the individual frequencies for signal forms, in particular pulses, which occur in practice.

Abstract: The present disclosure relates to phase measurement circuitry operable based on a first clock signal having an intended clock frequency F1 and a second clock signal having an intended clock frequency F2, the circuitry comprising: a delay line configured to receive the first clock signal, the delay line comprising a plurality of delay units each configured to cause a propagation delay, and the plurality of delay units connected in series along the length of the delay line and defining a series of positions therebetween through which signal edges of the first clock signal propagate over time; an edge detector configured to sample the delay line at successive sample times based on the second clock signal and to record at each sample time the position of a given signal edge of the first clock signal along the delay line; and a phase angle determiner configured to determine a phase angle per delay unit based on successive recorded said positions.

Abstract: Technology for adjusting touch resolution for a capacitive touch sensor. A touch resolution of a capacitive touch sensor is set to a first resolution. A footprint of an object in contact with a surface of the capacitive touch sensor is determined. The capacitive touch sensor is adjusted to a second resolution to detect objects based on the footprint of the object, wherein the second resolution is different than the first resolution.

Abstract: A measurement method for increasing the effective size of a quiet zone is provided. The measurement method comprises the step of in the case that an amplitude and/or a phase distribution of the quiet zone is known, determining a set of correction factors on the basis of said amplitude and/or phase distribution of the quiet zone. Additionally or alternatively in the case that the amplitude and/or phase distribution of the quiet zone and an antenna radiating aperture location on a device under test are known, the device under test is centered in the middle of the quiet zone and the device under test is moved radially in at least one axis.

Abstract: A measuring system for measuring properties of a device under test over the air comprises an antenna array, adapted to receive first measuring signals from the device under test, a measuring device adapted to process the first measuring signals received by the antenna array, and to generate second measuring signals and transmit them to the device under test, using the antenna array. The measuring device comprises a quiet zone generator, which is adapted to perform a beamforming of the first measuring signals after reception by the antenna array. It is preferably also adapted to perform a beamforming on the second measuring signals before transmission by the antenna array. The quiet zone generator is adapted to apply the beamforming, so that at least one adjustable quiet zone is achieved.

Abstract: The invention relates to a method for detecting an error in a generator unit which has an electric machine (100) with a rotor winding (110), a stator winding (120), and a rectifier (130) connected thereto, via which the electric machine (100) is connected to an onboard electrical system (150) of a motor vehicle, wherein a voltage of the onboard electrical system (150) is controlled to a target value via an excitation current (IE) through the rotor winding (110) of the electric machine (100), and a curve of a phase voltage (Uy) of the stator winding (120) is monitored, wherein an error in the generator unit is inferred when a change of the phase voltage (Uy) is detected and a voltage of the onboard electrical system (150) changing within a threshold value range (S) is detected.

Abstract: A system and method for an aircraft includes a probe, first and second current sensors, and a control circuit. The probe includes a heater that includes a resistive heating element routed through the probe, wherein an operational current is provided to the resistive heating element to provide heating for the probe. The first current sensor is configured to sense a first current through the resistive heating element, and the second current sensor is configured to sense a second current through the resistive heating element. The control circuit is configured to determine a leakage current based on the first and second currents and determine a remaining useful life the probe based on the leakage current over time.