Abstract: The present invention is directed to a method of making a live cell construct or a support, comprising: (a) providing a non-cellular organic polymer support having a top surface, a bottom surface, and an intermediate portion there between, and (b) contacting a cross-linking agent to one surface of said support for a time sufficient to generate a gradient of cross-linking of said polymer in said intermediate portion. Also provided are live cell constructs, supports, and methods of use of the supports and live cell constructs.

Abstract: There are suitability and unsuitability of a shape of a substrate used for cell culture, for each step in various cell culture protocols, and it has been difficult to continue providing a culture substrate shape suitable for each step. Provided is a cell culture system including: a culture sheet deformable by external force, to which a cell can adhere and be cultured; a die member configured to be pressed against the culture sheet to deform a shape of the culture sheet into a randomly-selected shape; an optical device configured to observe a state of the cell adhering onto the culture sheet; and a drive unit configured to move the die member, to change a randomly-selected portion of the culture sheet into a shape along the die member or release the culture sheet from deformation.

Abstract: A rocking mechanism for a bioreactor vessel includes a base, a motor mounted to the base and having an eccentric roller driven by the motor, and a rocking plate in contact with the eccentric roller, the rocking plate being configured to receive a bioreactor vessel thereon. The motor is controllable to drive the eccentric roller to transmit a force against an underside of the rocking plate to tilt the rocking plate and bioreactor vessel.

Abstract: A cell culture system includes a first component having an opening at the bottom, a membrane for diffusing a gas into a liquid by dissolution, an enclosure able to hold a volume of gas that can diffuse into the liquid via the membrane and a drive member for driving the container, the container being inclined at a non-zero angle ? that is less than or equal to 30°. A cell culture system comprising a first component and a second component forming a ring-shaped container and a drive member, the container being inclined at a non-zero angle ? that is less than or equal to 30°. A method using a system of the invention and to an assembly comprising a system of the invention and a liquid containing a species that is to be cultured.

Abstract: A foam identification system including a thermal imaging camera and a controller connected to the thermal imaging camera. The thermal imaging camera images a surface of a liquid in a vessel that is exposed to a headspace of the vessel. The headspace being either warmer or cooler than the liquid. The camera and the controller detect a change in temperature of the exposed surface of the liquid to identify foam on the exposed surface of the liquid.

Abstract: Methods and systems to control continuous gas fermentation platforms for improved conversion of gas substrates into products is developed and particularly relates to a control process and system to control feedstock gases and maximize the bioreactor performance with biomarkers. Improved carbon utilization results through providing the most beneficial feed of substrates to the bioreactor of the fermentation process.

Abstract: A method of providing sensing apparatus and a sensing method are disclosed. One aspect provides sensing apparatus comprising: a chamber configured to receive a biological sample at least part of a wall of the chamber comprising a printed circuit board substrate; the part of the wall comprising a printed circuit board substrate comprising an electrode configured to transmit or receive a signal to the biological sample, the electrode being coupleable to a sensing control unit located outside the chamber. The apparatus comprising a channel in communication with the chamber, the channel being dimensioned to allow at least a portion of the biological sample extend through the channel, the channel comprising a conductive surface configured to transmit or receive a signal to at least a portion of the biological cell sample extendable through the channel, the conductive channel surface being coupleable to the sensing control unit located outside the chamber.

Abstract: The present disclosure provides cell culture chambers for use in automated cell engineering systems, and in particular, cell culture chambers that provide increased cell yield. A cell culture chamber may include a first body forming a first portion of the cell culture chamber. A cell culture chamber may include a second body forming a second portion of the cell culture chamber, the first body and the second body configured to couple together, forming an enclosed volume, wherein a first non-porous, gas-permeable material is disposed on the first body; and wherein the first non-porous, gas-permeable material and the first body are formed together. A second non-porous, gas-permeable material may be disposed on the second body, and the second non-porous, gas-permeable material and the second body may be formed together. The cell culture chamber may further include at least one bubble trap integrally formed with the first body and/or the second body.

Abstract: Disclosed herein include methods, compositions, and kits suitable for use in processing a sample comprising blood cells and at least one microorganism. In some embodiments, the method comprises contacting the sample with a lysis buffer to generate a treated sample. The lysis buffer can comprise a Somatic Cell Digestion Agent (SDA) capable of lysing blood cells in the sample. In some embodiments, the at least one microorganism remains intact and/or viable in the presence of the SDA.

Abstract: The present disclosure relates to methods and compositions for producing a recombinant component for use in a food or other product. The present disclosure further relates to compositions comprising the recombinant component produced by such methods and compositions.

Abstract: Provided herein, in some embodiments, are compositions and methods for a plasmid addiction system based on essential glycolytic genes. Also provided herein, in some embodiments, are compositions and methods for a plasmid addiction system based on an outer membrane efflux protein.

Abstract: Present invention relates to dried lactic acid bacteria compositions are stabilised with synergistic mixtures of stabilisers selected from oligofructans, maltodextrin, inulin and pea fibre. The mixtures have been found to stabilise the compositions during the drying (e.g. freeze-drying) process and during storage.

Abstract: The present disclosure relates to a Corynebacterium sp. mutant strain having increased L-arginine or L-citrulline productivity and a method of producing L-arginine or L-citrulline using the same. The Corynebacterium sp. mutant strain has enhanced activity of acetylglutamate kinase involved in the L-arginine biosynthesis pathway, and thus is capable of producing L-arginine or L-citrulline in an increased yield compared to a parent strain.

Abstract: The present disclosure relates to a Corynebacterium sp. mutant strain having increased L-arginine or L-citrulline productivity and a method of producing L-arginine or L-citrulline using the same. The Corynebacterium sp. mutant strain has enhanced activity of acetylornithine aminotransferase involved in the L-arginine biosynthesis pathway, and thus is capable of producing L-arginine or L-citrulline productivity in an increased yield compared to a parent strain.

Abstract: A method is disclosed for coating and patterning hydrogels in order to modify surface properties. The method exploits the water content of the hydrogel and the hydrophobicity of the reaction solvent to create a thin oxide adhesion layer on the hydrogel surface. This oxide adhesion layer enables rapid transformation of the hydrophilic, cell non-adhesive hydrogel into either a highly hydrophobic or a cell-adhesive hydrogel by reaction with an alkylphosphonic acid or an ?,?-diphosphonoalkane, respectively. Also disclosed are coated, patterned hydrogels and constructs comprising the coated, patterned hydrogels.

Abstract: The present disclosure discloses a culture device and method. The culture device includes a substrate, wherein a surface of the substrate includes a plurality of culture regions. Each of the plurality of culture regions are configured to adhere a culture. An adhesion affinity of an interior of each of the plurality of culture regions is greater than an adhesion affinity of an exterior of each of the plurality of culture regions. The present disclosure further discloses a culture method for biological culture using the culture device.

Abstract: The present invention relates to a method of producing cancer stem cells that comprises culturing a living cell population containing cancer cells in the presence of a gel substance to obtain a living cell population containing cancer stem cells, wherein the gel substance is a material that induces expression of osteopontin in at least a portion of cells contained in the living cell population. Moreover, the present invention relates to an agent for inducing conversion of cancer cells to cancer stem cells that comprises a gel substance that induces expression of osteopontin in at least a portion of the cells contained in a living cell population. The gel substance is a synthetic polymer gel composed of, for example, double network gel, PNaSS gel, PCDME gel, PA gel, PAMPS gel, PDMA gel or PAAc gel. The present invention provides means and a method that enable the preparation of cancer stem cells in a relatively short period of time and at a relatively low culturing cost without requiring expensive equipment.

Abstract: A cell culture article includes a substrate having a polymer coating that is conducive to colony passaging of cells cultured on the coating. Example polymer coatings are formed from polygalacturonic acid (PGA), alginate, or combinations thereof. Cells cultured on the polymer coating can be separated from the substrate as a colony or layer of cells by exposing the polymer coating to (i) a chelating agent, (ii) a proteinase-free enzyme, or (iii) a chelating agent and a proteinase-free enzyme.

Abstract: The invention relates to a method for separating viable target cells from a sample comprising the steps of contacting a sample comprising a suspension of viable target cells that display a molecule on the cell surface with non-porous microparticles that have a density of about 1.

Abstract: An osmotically-active closed-cell composite comprises a closed-cell structure including fluid-filled cells separated by cell walls, where the fluid-filled cells comprise water and a solute, and the cell walls comprise a polymer permeable to water and impermeable to the solute. The closed-cell structure is configured to undergo osmotically-induced swelling during exposure to an aqueous environment having a different chemical potential from the fluid-filled cells.

Abstract: Disclosed herein are compositions and methods for treating, ameliorating or preventing a retinal disease or condition; improving a photopic (day light) vision; for improving correcting visual acuity, improving macular function, improving a visual field, or improving scotopic (night) vision by administration of retinal progenitor cells. The subject matter described herein also provides cell populations comprising retinal progenitor cells and methods of isolation thereof.

Abstract: The invention relates to a device (1) for preparing a cell suspension comprising an enzyme reservoir (10), a first purification compartment (20) for receiving a first purification material (P1), wherein the first purification compartment (20) is in fluid connection or can be brought into fluid connection with the enzyme reservoir (10), a syringe (40) comprising a barrel (41) defining a barrel compartment (45) and a piston (42) which is movably arranged in the barrel compartment (45), wherein the syringe (40) comprises a syringe inlet (43) connected to the barrel compartment (45), wherein the syringe inlet (43) is in fluid connection or can be brought into fluid connection with the first purification compartment (20), and wherein the syringe (40) comprises a syringe outlet (44), and wherein the device (1) comprises a flow path from the enzyme reservoir (10) via the first purification compartment (20) to the syringe inlet (43). Also provided herein are methods for preparing a cell suspension.

Abstract: The present invention provides genetically modified lymphocyte with reduced cytotoxicity. Also provided are methods of using these genetically modified lymphocytes to deliver cargo molecules to a target cell.

Abstract: The present invention relates to compositions and methods for enhancing T cell metabolism and activity for more effective adoptive T cell therapy. By expressing an intracellular signaling molecule in T cells, the T cells are metabolically enhanced with improved cytotoxicity and resistance to immunosuppression imposed by tumor microenvironments. One aspect includes a modified T cell and pharmaceutical compositions comprising the modified cells for adoptive cell therapy and treating a disease or condition associated with enhanced immunity.

Abstract: The present disclosure generally relates target cancer cell specific immunotherapy compositions, methods of making and use thereof. Also provided in the disclosure are methods of treating a cancer in a subject in need thereof.

Abstract: Provided is the use of a compound for improving the transplantation efficiency of human hematopoietic stem cells, wherein the compound is selected from one of or two or more of a tubulin polymerization inhibitor, LND-212854, AZD0364, SCH772984, Pimasertib, Trametinib, IWR-1-endo, TTNPB, JNK-inhibitor IX and CHIR99021. The compound provided can improve the expression of the hematopoietic stem cell surface protein CD184 by means of in vitro short-time treatment, without affecting the phenotype, survival rate and various characteristics of the cell. By means of the binding of the chemokine receptor CD184 expressed by HSC to the chemokine ligand SDF1 in the bone marrow microenvironment, the migration ability of the HSC and the homing ability thereof in the bone marrow are improved, and the transplantation efficiency and the therapeutic effect of the HSC are enhanced.

Abstract: A glass-ceramic composition as defined herein. The glass-ceramic composition includes a first crystalline phase comprising lithium disilicate. The glass-ceramic composition can include up to 10 wt % CaO, up to 5 wt % Na2O, up to 10 wt % B2O3, and greater than 0.5 wt % ZrO2. The glass-ceramic composition can also include from 50 to 75 wt % SiO2, from 1 to 5 wt % Al2O3, from 1 to 8 wt % P2O5, and from 5 to 20 wt % Li2O. In aspects, the glass-ceramic composition can include a second crystalline phase including wollastonite, apatite, cristobalite, ?-quartz, lithiophosphate, or a combination thereof. Also disclosed are methods of making and using the disclosed compositions.

Abstract: Disclosed herein is a simple and reproducible 3D-culture-based process for generating cardiac spheroids containing all four cardiac-cell types (cardiomyocytes, endothelial cells, smooth muscle cells, and cardiac fibroblasts) that is compatible with a wide range of applications and research equipment. Subsequent experiments demonstrated that the inclusion of vascular cells and cardiac fibroblasts was associated with an increase in spheroid size, a decline in apoptosis, an improvement in sarcomere maturation and a change in CM bioenergetics. These suggest a three-dimensional (3D) environment with endothelial cells, smooth muscle cells, and cardiac fibroblasts which promoted CM maturation and electrical activity.

Abstract: A method for aligning and culturing cells in three dimensions includes mixing cells with a hydrogel to prepare a bio-ink, outputting the bio-ink while moving a nozzle of a discharge device to form an output product having a pattern having at least parallel portions, curing the output product, and culturing the cured product. According to the present invention, cells may be cultured to a high density in a three-dimensionally aligned state along a parallel pattern, so that there is an advantage in that the texture of a tissue formed by the cells, for example, a unique texture of a muscle tissue, may be simulated or reproduced.

Abstract: Methods and products obtained from the method for culturing stem cells and tissues using stem cells, cord tissue fragments, or stem cells, decellularized cord tissue fragments, or mixed cells from cord tissue or combinations thereof to amplify stem cells are described.

Abstract: The present disclosure relates to a scaffold for culture and transplantation of a kidney organoid using a decellularized kidney tissue (KEM).

Abstract: Methods for generating CD31+VE?cadherin+KDR+ endothelial cells (ECs) are provided using chemically-defined culture media that allow for generating ECs from early mesoderm progenitors in as little as three days and from pluripotent stem cells in as little as five days. Culture media, isolated cell populations and kits are also provided.

Abstract: Methods for generating human arterial endothelial cells under defined conditions in the absence of insulin are described.

Abstract: Described herein are various systems, methods, and apparatus for systematic creation of isolated homogeneous colonies of cells from vector-based lineages. The vector-based lineages may originate from multiple types of viral vector families (e.g., Paramyx-oviridae, Retroviridae, Parvoviridae) or non-natural engineered vectors or a plurality of vector combinations, for example. In certain embodiments, the isolated homogeneous colonies of cells are vector-free sub-colonies; in other embodiments, the isolated homogeneous colonies of cells are homogeneous vector sub-colonies. In other embodiments, vector mixed sub-colonies are created. The disclosed systems, methods, and apparatus are useful for inducible pluripotent stem cell (iPSC) production and work by selectively binding to one or more corresponding protein markers expressed on the surface of a cell that indicate that cellular reprogramming has occurred. Software is used to automate the purification and isolation of the iPSCs produced.

Abstract: Provided is a method for producing a stem cell clone, which comprises the steps of: (i) introducing into stem cells an exogenous gene associated with induction of differentiation into somatic cells; (ii) inducing differentiation of the stem cells, introduced with an exogenous gene, into the somatic cells; (iii) dedifferentiating the differentiation-induced somatic cells; and (iv) isolating stem cells having the exogenous gene incorporated into a chromosome thereof from a colony of the stem cells formed in step (iii).

Abstract: Provided herein are recombinant viruses and artificially coated delivery systems, and methods of use.

Abstract: The present disclosure provides an automated method of producing viral vectors, utilizing engineered viral vector-producing cell lines within a fully-enclosed cell engineering system. Exemplary viral vectors that can be produced include lentivirus vectors, adeno-associated virus vectors, baculovirus vectors and retrovirus vectors.

Abstract: This invention relates to methods and materials for nucleic acid delivery, vaccination, and/or treating cancer. More specifically, methods and materials for nucleic acid delivery, vaccination, and/or treating cancer using one or more recombinant adenoviruses (Ads) as an oncolytic agent are provided.

Abstract: The objective of the present invention is to provide a novel bacteriophage useful for treating bacterial endophthalmitis and a therapeutic agent for bacterial endophthalmitis comprising the novel bacteriophage. The therapeutic agent for bacterial endophthalmitis according to the present invention is characterized in comprising 1 or more bacteriophages selected from the group essentially consisting of Myoviridae Spounavirinae phiEF7H (accession number: NITE BP-02886), Myoviridae Spounavirinae phiEF19G (accession number: NITE BP-02887), Myoviridae Spounavirinae phiEF14H1 (accession number: NITE BP-02888), and mutants thereof.

Abstract: This invention relates to a HEK-293 cell line that grows under animal component-free suspension conditions. This invention further relates a HEK-293 cell line that grows under adherent conditions. The cell lines may provide rapid and scalable production of adenoassociated virus (AAV) and support production of all serotypes and chimera of AAV.

Abstract: The present invention relates to the field of prevention and/or treatment of metabolic disorders such as obesity, metabolic syndrome, type-2 diabetes, dyslipidemia and insulin resistance,

Abstract: Provided is a technique for improving the translation efficiency of mRNA. The mRNA includes: a 5? untranslated region of an mRNA encoding a protein; and a 3? untranslated region having 40% or more and 80% or less complementarity to the 5? untranslated region.

Abstract: The subject invention provides a method for purifying a target protein from a mixture comprising the target protein and contaminating protein, comprising the steps of exposing the mixture to an effective amount of a cationic surfactant such that the contaminating protein is preferentially precipitated and recovering the target protein. Proteins purified according to the method of the invention are also provided.

Abstract: The present invention provides improved P450-BM3 variants with improved activity. In some embodiments, the P450-BM3 variants exhibit improved activity over a wide range of substrates.

Abstract: Described herein is a polypeptide encoding a prenyltransferase for prenylating a polyphenol and a polypeptide encoding an O-methyltransferase for methylating a polyphenol. For example, the polypeptide comprises or consists of the sequence of SEQ ID NO: 1, 2, 3, 4, 5, and/or 6, and/or a polypeptide listed in Table 1, and/or SEQ ID NO: 7-30, or a variant thereof having at least 80% sequence identity to SEQ ID NO: 1, 2, 3, 4, 5, and/or 6, and/or the polypeptide listed in Table 1, and/or SEQ ID NO: 7-30, or a fragment of the polypeptide or the variant thereof.

Abstract: Provided are mutant polymerases having DNA polymerase activity and reverse transcriptase activity or strand displacement activity, along with target nucleic acid amplification methods employing such mutant polymerases.

Abstract: The present disclosure provides methods and systems for amplifying and analyzing nucleic acid samples. The present disclosure provides methods for preparing cDNA and/or DNA molecules and cDNA and/or DNA libraries using modified reverse transcriptases.

Abstract: Compositions and methods are provided for potent mRNA vaccines for treatment of cancers with a mutation in the ras gene family. The compositions include a pharmaceutical composition comprising, or consisting essentially of, or yet further comprising mRNA molecules encoding at least one of multiple peptides of a group of somatic mutants and a pharmaceutically acceptable carrier. Methods for stimulating system immune responses and treatment are provided, including intratumoral, intravenous, intramuscular, intradermal, or subcutaneous injection of the composition as disclosed herein.

Abstract: The present invention relates to methods for treating ischemic events in a patient, especially ST-segment elevation myocardial infarction and acute ischemic stroke, by administrating a recombinant apyrase protein in conjunction with a P2Y12 inhibitor.

Abstract: The present invention relates to plant-produced angiogenins, to related plant cells, plant calli, plants, seeds and other plant parts and products derived therefrom and to uses of plant-produced angiogenins. The present invention also relates to expression of angiogenin genes in plants and to related nucleic acids, constructs and methods.