Patents Issued in February 29, 2024
  • Publication number: 20240067974
    Abstract: The present invention provides a method for the treatment of autosomal dominant Catecholaminergic Polymorphic Ventricular Tachycardia associated with mutations in the cardiac ryanodine receptor type 2 (RYR2) gene, by the use of an AAV mediated RNA interference approach to induce allele specific silencing of mutant mRNA.
    Type: Application
    Filed: February 27, 2023
    Publication date: February 29, 2024
    Inventors: Silvia G. PRIORI, Rossana BONGIANINO, Marco DENEGRI, Carlo NAPOLITANO
  • Publication number: 20240067975
    Abstract: A method for controlling expression of a nuclear-encoded gene in a plant comprising expressing a chloroplast-encoded dsRNA that silences an endogenous nuclear-encoded gene in the plant to produce a transformed plant line expressing the selected trait.
    Type: Application
    Filed: May 3, 2023
    Publication date: February 29, 2024
    Inventor: Jeffrey Staub
  • Publication number: 20240067976
    Abstract: The present invention relates to a wild-type-derived introgression line of a tomato in which the expression of BC2.1 is inhibited and the amount of beta-carotene or lutein is increased; and a transgenic tomato.
    Type: Application
    Filed: December 23, 2021
    Publication date: February 29, 2024
    Applicant: KYUNGPOOK NATIONAL UNIVERSITY INDUSTRY-ACADEMIC COOPERATION FOUNDATION
    Inventor: Je Min LEE
  • Publication number: 20240067977
    Abstract: The present disclosure provides composition and methods for improving leaf quality of low-alkaloid tobacco plants. Also provided are the identification and genetic engineering of target genes (Leaf Quality Genes) for producing tobacco plants with altered total alkaloid and nicotine levels and commercially acceptable leaf grade, their development via breeding or transgenic approaches, and production of tobacco products from these tobacco plants.
    Type: Application
    Filed: October 9, 2020
    Publication date: February 29, 2024
    Applicant: ALTRIA CLIENT SERVICES LLC
    Inventors: Chengalrayan KUDITHIPUDI, Rajanikanth GOVINDARAJULU, Nazmul BHUIYAN, Raija PAYYAVULA, Yanxin SHEN, Dongmei XU
  • Publication number: 20240067978
    Abstract: The present invention provides nucleic acids encoding transcription factors and methods of using these nucleic acids to modulate nicotine production in plants and to produce plants having modulated nicotine production.
    Type: Application
    Filed: September 21, 2023
    Publication date: February 29, 2024
    Inventors: Rongda Qu, Bingwu Wang
  • Publication number: 20240067979
    Abstract: The present disclosure relates to tobacco plants, tobacco seeds, compositions, and methods related to the identification and introgression of the Pale Yellow locus in tobacco. It also relates to generating novel mutations within the PY locus in tobacco.
    Type: Application
    Filed: October 24, 2023
    Publication date: February 29, 2024
    Inventors: Sreepriya PRAMOD, Andrew C. ADAMS, Marcos Fernando DE GODOY LUSSO, Gregory A. DAVIS, Jerry W. MORRIS, Dongmei XU, Jesse FREDERICK
  • Publication number: 20240067980
    Abstract: This disclosure relates to stay-green plants and plants with increased agricultural productivity, as well as to methods for obtaining same.
    Type: Application
    Filed: October 17, 2023
    Publication date: February 29, 2024
    Inventors: Dror SHALITIN, Noam GRIMBERG, Aviva KATZ
  • Publication number: 20240067981
    Abstract: Methods and materials for modulating low-nitrogen tolerance levels in plants are disclosed. For example, nucleic acids encoding low nitrogen tolerance-modulating polypeptides are disclosed as well as methods for using such nucleic acids to transform plant cells. Also disclosed are plants having increased low-nitrogen tolerance levels and plant products produced from plants having increased low-nitrogen tolerance levels.
    Type: Application
    Filed: August 14, 2023
    Publication date: February 29, 2024
    Inventors: Gregory Nadzan, Richard Schneeberger, Han Suk Kim, David Van-Dinh Dang, Kenneth A. Feldmann
  • Publication number: 20240067982
    Abstract: Disclosed are components and methods for RNA-directed DNA cleavage and gene editing. The components include and the methods utilize a Cas9 protein from Neisseria and one or more RNA molecules in order to direct the Cas9 protein to bind to and optionally cleave or nick a target sequence.
    Type: Application
    Filed: November 23, 2022
    Publication date: February 29, 2024
    Inventors: Erik J. Sontheimer, Yan Zhang, Alfonzo Mondragon, Rakhi Rajan, James Thomson, Zhonggang Hou
  • Publication number: 20240067983
    Abstract: A novel gRNA-ligand binding complex is provided. This complex may be used to bring Type V Cas proteins and additional effectors to DNA for base editing. The design of the systems allows for the production of efficient modular components that provide flexibility when editing DNA.
    Type: Application
    Filed: January 5, 2022
    Publication date: February 29, 2024
    Inventors: Kurt Daniel Marshall, Hide Bueno Machado, Emily Anderson, Alexander Hale, Amanda Smith, Anastasia Kaufman, Leah Nantie, Michael Daniel Rushton, Kevin Hemphill
  • Publication number: 20240067984
    Abstract: The present disclosure provides isolated promoters, transgene expression cassettes, vectors, kits, and methods for treatment of C9ORF72 associated diseases, including ALS and FTD.
    Type: Application
    Filed: April 24, 2023
    Publication date: February 29, 2024
    Inventors: Peixin Zhu, Xijia Wang, Steven Pennock, Mark Shearman
  • Publication number: 20240067985
    Abstract: Disclosed herein are chimpanzee adenoviral vectors that include neoantigen-encoding nucleic acid sequences derived from a tumor of a subject. Also disclosed are nucleotides, cells, and methods associated with the vectors including their use as vaccines.
    Type: Application
    Filed: January 10, 2023
    Publication date: February 29, 2024
    Inventors: Wade Blair, Brendan Bulik-Sullivan, Jennifer Busby, Adnan Derti, Leonid Gitlin, Karin Jooss, Ciaran Daniel Scallan, Roman Yelensky, Gijsbert Grotenbreg
  • Publication number: 20240067986
    Abstract: AAV capsid proteins comprising a modification in the amino acid sequence and virus vectors comprising the modified AAV capsid protein are described. Also described are methods of administering the virus vectors and virus capsids to a cell or to a subject in vivo.
    Type: Application
    Filed: September 25, 2023
    Publication date: February 29, 2024
    Inventors: Daniel McCoy, Garrett E. Berry
  • Publication number: 20240067987
    Abstract: The present disclosure provides a variant AAV capsid protein that confers tropism to lung cells and recombinant adeno-associated viruses comprising the variant A.AV and pharmaceutical compositions comprising same and their use in the delivery of heterologous nucleic acids to lung cells for the treatment of pulmonary disorders.
    Type: Application
    Filed: September 15, 2023
    Publication date: February 29, 2024
    Applicant: 4D MOLECULAR THERAPEUTICS INC.
    Inventors: Melissa Kotterman, Peter Francis, Melissa Calton, Johnny Gonzales, Roxanne Croze, Christopher Schmitt
  • Publication number: 20240067988
    Abstract: The present disclosure provides adeno-associated virus (AAV) vectors, comprising coevolved capsid variant proteins, pharmaceutical compositions, methods of making, and methods for delivering such to a subject.
    Type: Application
    Filed: September 26, 2023
    Publication date: February 29, 2024
    Applicant: Duke University
    Inventors: Aravind Asokan, Trevor Gonzalez, Lawrence Patrick Havlik
  • Publication number: 20240067989
    Abstract: The present invention relates to the prevention and/or treatment of retinal disorders, such as cone dystrophies, cone-rod dystrophies, in particular Achromatopsia.
    Type: Application
    Filed: June 16, 2023
    Publication date: February 29, 2024
    Applicant: UCL BUSINESS LTD
    Inventors: Robin Ali, Takaaki Matsuki, Alexander Smith, Anastasios Georgiadis
  • Publication number: 20240067990
    Abstract: The present invention relates to polymeric micelle compositions. More specifically, the present invention relates to polymeric micelle compositions for gene delivery.
    Type: Application
    Filed: August 25, 2023
    Publication date: February 29, 2024
    Inventors: MATTHEW MOFFIT, SUNDIATA KLY
  • Publication number: 20240067991
    Abstract: Certain aspects of the present disclosure provides systems, compositions, and methods for regulating expression or activity of an endogenous cytokine of a cell. In some cases, the present disclosure provides a system comprising an actuator moiety capable of complexing with a target gene encoding the endogenous cytokine to regulate expression or activity of the endogenous cytokine. The actuator moiety can be heterologous to the cell. The actuator moiety can be activatable upon exposing the cell to an external stimulus. Upon the exposure of the cell to the external stimulus, the actuator moiety can be activated to regulate expression or activity of the endogenous cytokine.
    Type: Application
    Filed: May 2, 2023
    Publication date: February 29, 2024
    Applicant: FUNDAÇÃO D. ANNA DE SOMMER CHAMPALIMAUD E DR. CARLOS MONTEZ CHAMPALIMAUD FOUNDATION
    Inventors: Maggie L. BOBBIN, Vitaly BALAN, Rona HARARI-STEINFELD, Francesco M. MARINCOLA, Zhifen YANG
  • Publication number: 20240067992
    Abstract: Methods and compositions useful in targeting a payload to or editing target nucleic acid are disclosed herein.
    Type: Application
    Filed: August 16, 2023
    Publication date: February 29, 2024
    Applicant: Editas Medicine, Inc.
    Inventors: Alexandra GLUCKSMANN, Deborah PALESTRANT, Louis Anthony TARTAGLIA, Jordi MATA-FINK, Agnieszka Dorota CZECHOWICZ
  • Publication number: 20240067993
    Abstract: The present invention provides a method for anaerobic culture of denitrifying microorganisms in culture medium in which nitric acid and/or nitrous acid are added two or more times to the medium during culture, and in which the pH of the medium is maintained within a pH interval by the addition of the nitric acid and/or nitrous acid and the nitric acid and/or nitrous acid also provides the electron acceptor for denitrification in the culture. Methods of producing a cell culture product using such methods and the products which are obtained are also provided.
    Type: Application
    Filed: January 5, 2022
    Publication date: February 29, 2024
    Applicant: NORWEGIAN UNIVERSITY OF LIFE SCIENCES
    Inventors: Lars Reier BAKKEN, Linda BERGAUST, Svein Jarle HORN
  • Publication number: 20240067994
    Abstract: The disclosure provides a process for the production of ethanol and one or more low boiling compound from a fermentable carbon source. The ethanol and the low boiling compound(s) are produced using an ethanol-producing yeast modified to further produce the one or more low boiling point compounds. In one embodiment, the low boiling compound(s) are acetone, 1-propanol, and/or 2-propanol. Additionally, the disclosure provides a process for the isolation ad purification of the one or more low boiling compounds from ethanol.
    Type: Application
    Filed: August 23, 2023
    Publication date: February 29, 2024
    Inventors: Renata PINTO DA SILVA MATOS, Thiago BEZERRA TAKETA, Felipe GALZERANI, Adler GOMES MOURA
  • Publication number: 20240067995
    Abstract: A foodstuff can include a free acid ?-hydroxybutyrate, and a base. The ?-hydroxybutyrate, and base, are present at a less than 1:1 molar equivalence.
    Type: Application
    Filed: April 11, 2023
    Publication date: February 29, 2024
    Inventor: Frank Borges LLOSA
  • Publication number: 20240067996
    Abstract: The invention provides a Saccharomyces cerevisiae strain for producing a human milk lipid substitute. By integrating a heterologous lysophosphatidic acid acyltransferase into Saccharomyces cerevisiae and knocking out its own natural lysophosphatidic acid acyltransferase, the content of palmitic acid (C16:0) at Sn-2 position of triacylglycerol produced by Saccharomyces cerevisiae is increased, to synthesize a human milk lipid substitute. On this basis, a metabolic pathway related gene is knocked out, to further increase the content of human milk lipid substitute in the product. In the present invention, a human milk lipid substitute is de novo synthesized by Saccharomyces cerevisiae for the first time, in which the total fatty acid is 15% or more, and the relative content of C16:0 at Sn-2 position reaches about 60%.
    Type: Application
    Filed: November 1, 2023
    Publication date: February 29, 2024
    Inventors: Long LIU, Jian Chen, Xueqin LV, Wenyang Wu, Guocheng Du, Jianghua Li, Guolin Zhou, Yanfeng Liu, Chenyang Zhang
  • Publication number: 20240067997
    Abstract: The disclosure relates to host cells having altered NADPH availability, allowing for increased production of compounds produced using NADPH, and methods of use thereof. NADPH availability is altered by one or more of: expressing an altered GAPDH, expressing a variant glutamate dehydrogenase (gdh), aspartate semialdehyde dehydrogenase (asd), dihydropicolinate reductase (dapB), and meso-diaminopimelate dehydrogenase (ddh), expressing a novel nicotinamide nucleotide transhydrogenase, expressing a novel threonine aldolase, and expressing or modulating the expression of a pyruvate carboxylase in the host cells.
    Type: Application
    Filed: October 7, 2022
    Publication date: February 29, 2024
    Inventors: Shawn MANCHESTER, Benjamin MASON, Alexi GORANOV
  • Publication number: 20240067998
    Abstract: Disclosed are strain having enhanced L-glutamic acid production capacity, and method for constructing the same and use thereof. A nucleotide sequence is provided by introducing a point mutation to a wild-type BBD29-00405 gene in Corynebacterium glutamicum so that the base at position 597 of SEQ ID NO: 1 is mutated from guanine (G) into adenine (A). Also provided is a recombinant strain obtained by introducing the polynucleotide sequence into L-glutamic acid-producing Corynebacterium glutamicum, the recombinant strain comprising a BBD29-00405 gene containing a point mutation. Compared with an unmodified strain, the resulting strain facilitates production of L-glutamic acid at a higher concentration.
    Type: Application
    Filed: December 29, 2021
    Publication date: February 29, 2024
    Applicant: NINGXIA EPPEN BIOTECH CO., LTD
    Inventors: Houbo SU, Aiying WEI, Gang MENG, Lipeng YANG, Fengyong MA, Huiping JIA, Xiaoqun ZHOU, Chunguang ZHAO
  • Publication number: 20240067999
    Abstract: A recombinant strain with modified gene BBD29_11265 and a method for constructing the same are provided. The recombinant strain is a bacterium that generates L-glutamic acid, and has an improved expression of a polynucleotide encoding an amino acid sequence of SEQ ID NO: 3 or a homologous sequence thereof; the improved expression can be having a point mutation in, and an enhanced expression of the polynucleotide encoding an amino acid sequence of SEQ ID NO: 3 or a homologous sequence thereof. A genetically engineered bacterium in which the base at position 70 in the BBD29_112665 gene sequence is mutated to adenine from guanine, causing alanine at position 24 in the coded corresponding amino acid sequence to be substituted with threonine, and an engineered bacterium overexpressing the BBD29_112665 gene or BBD29_11265G70A gene are constructed in the present invention, facilitating an increase in the production and conversion rate of L-glutamic acid.
    Type: Application
    Filed: December 28, 2021
    Publication date: February 29, 2024
    Applicant: NINGXIA EPPEN BIOTECH CO., LTD
    Inventors: Aiying WEI, Gang MENG, Chunguang ZHAO, Huiping JIA, Houbo SU, Lipeng YANG, Xiaowei GUO, Bin TIAN, Fengyong MA, Xiaoqun ZHOU
  • Publication number: 20240068000
    Abstract: The present invention relates to an E. coli hisG-derived ATP-phosphoribosyltransferase variant having a reduced feedback inhibition by histidine and a strain expressing the same. The variant may maintain its activity even at a high histidine concentration, thus increasing histidine production.
    Type: Application
    Filed: April 26, 2021
    Publication date: February 29, 2024
    Applicant: DAESANG CORPORATION
    Inventors: Jong Yun HAN, Chel Min YANG, Yong Soo KIM, Young Il JO
  • Publication number: 20240068001
    Abstract: The disclosure discloses a method for chemically synthesizing a Helicobacter pylori core lipopolysaccharide oligosaccharide antigen carbohydrate chain, and belongs to the technical field of carbohydrate chemistry. The disclosure uses D-glucose, D-galactose and D-mannose as starting materials, which undergo a series of protection and deprotection reactions to prepare eight monosaccharide blocks. The eight monosaccharide block compounds as shown in formulas 2 to 9 undergo glycosylation reactions under catalysis of corresponding activating reagents, to prepare H. pylori lipopolysaccharide core oligosaccharide antigen fragments as shown in formula 1. The disclosure further combines the synthesized oligosaccharide fragments with a chip to make a carbohydrate chip, screens the optimal antigen fragments using patient serum, or combines the synthesized oligosaccharide fragments with carrier proteins to make glycoconjugates.
    Type: Application
    Filed: September 27, 2023
    Publication date: February 29, 2024
    Inventors: Jian YIN, Jing HU, Xiaopeng ZOU
  • Publication number: 20240068002
    Abstract: The present invention relates to a recombinant microorganism comprising one or more nucleotide sequence(s) encoding: a polypeptide having ent-copalyl pyrophosphate synthase activity; a polypeptide having ent-Kaurene synthase activity; a polypeptide having ent-Kaurene oxidase activity; and a polypeptide having kaurenoic acid 13-hydroxylase activity, whereby expression of the nucleotide sequence(s) confer(s) on the microorganism the ability to produce at least steviol.
    Type: Application
    Filed: June 23, 2023
    Publication date: February 29, 2024
    Inventors: Viktor Marius BOER, Erwin SUIR
  • Publication number: 20240068003
    Abstract: Described herein are engineered organelles comprising multi-component proteins from different species incorporated into a membrane structure with interior and exterior aspects. In one embodiment the artificial organelle incorporates one or more protein complexes that absorb optical energy and catalyze electron transfer in biochemical reactions that can be used to reduce NAD+ to NADH or analogues thereof.
    Type: Application
    Filed: September 21, 2023
    Publication date: February 29, 2024
    Inventors: Kyle A. Minor, Carlo D. Montemagno, David W. Wendell
  • Publication number: 20240068004
    Abstract: Provided herein are methods and cell-free systems for production of a recombinant antibody in which the antibody is directed against a target protein that is natively expressed as an intracellular protein. Also provided herein are modified cell-free systems for use in such methods in which the cell free protein expression system is modified by an agent which binds to the intracellular target protein or to the antibody paratope and thus prevents the antibody from binding to the target protein.
    Type: Application
    Filed: May 2, 2023
    Publication date: February 29, 2024
    Applicant: MBrace Therapeutics, Inc.
    Inventors: Yongjian Wu, Fernanda I. Staquicini, Isan Chen
  • Publication number: 20240068005
    Abstract: The present invention provides engineered ketoreductase and phosphite dehydrogenase enzymes having improved properties as compared to a naturally occurring wild-type ketoreductase and phosphite dehydrogenase enzymes, as well as polynucleotides encoding the engineered ketoreductase and phosphite dehydrogenase enzymes, host cells capable of expressing the engineered ketoreductase and phosphite dehydrogenase enzymes, and methods of using the engineered ketoreductase and phosphite dehydrogenase enzymes to synthesize a chiral catalyst used in the synthesis of antiviral compounds, such as nucleoside inhibitors. The present invention further provides methods of using the engineered enzymes to deracemize a chiral alcohol in a one-pot, multi-enzyme system.
    Type: Application
    Filed: July 14, 2023
    Publication date: February 29, 2024
    Inventors: Jeffrey C. Moore, Jack Liang, Jonathan Penfield, Jovana Nazor, Nikki Dellas, Vesna Mitchell, Da Duan, Iman Farasat, Agustina Rodriguez-Granillo, Grant Murphy, Nicholas Marshall
  • Publication number: 20240068006
    Abstract: A method for assessing drug-resistant Klebsiella pneumoniae includes the following steps. A test sample is provided, wherein the test sample includes a Klebsiella pneumoniae. A spectrum analysis step is performed, wherein the test sample is detected by a mass spectrometry method so as to obtain a target mass spectrum data. An assessing step for drug-resistant Klebsiella pneumoniae is performed, wherein the target mass spectrum data is analyzed so as to assess whether the Klebsiella pneumoniae is resistant to a carbapenem antibiotic or a colistin or not. When the Klebsiella pneumoniae is resistant to the carbapenem antibiotic, the target mass spectrum data includes a first anti-carbapenem feature mark, and when the Klebsiella pneumoniae is resistant to the colistin, the target mass spectrum data includes a first anti-colistin feature mark.
    Type: Application
    Filed: January 16, 2023
    Publication date: February 29, 2024
    Applicant: China Medical University
    Inventors: Der-Yang Cho, Po-Ren Hsueh, Jiaxin Yu, Ni Tien, Hsiu Hsien Lin, Chia-Fong Cho
  • Publication number: 20240068007
    Abstract: Provided herein are biomarkers associated with risk of bacterial infection, particularly hospital inquired infections, such as following surgeries. In particular, provided herein are fecal biomarkers (e.g., microbiomic, metabolomic, etc.) that correlate with elevated risk of infection in subjects following surgery (e.g., organ transplant surgery), and methods of treating and/or preventing such infections.
    Type: Application
    Filed: August 21, 2023
    Publication date: February 29, 2024
    Inventors: Eric Gerd Pamer, Nicholas Dylla, Christopher Lehmann
  • Publication number: 20240068008
    Abstract: The present invention relates generally to materials and methods for detection of bacteria, and for testing and determination of antibiotic susceptibility of bacteria in specimens of bodily fluid and other samples. The invention also relates to materials and methods for monitoring the physiological response of bacteria to antimicrobial agents, and for reducing background and increasing sensitivity of assays that involve the detection and/or measurement of RNA, such as rRNA. The invention provides kits comprising an RNase packaged for use in the methods described herein.
    Type: Application
    Filed: August 21, 2023
    Publication date: February 29, 2024
    Inventors: David A. Haake, Daniel Gussin, Gabriel K. Monti, Bernard M. Churchill, Colin W. Halford
  • Publication number: 20240068009
    Abstract: Described are substituted imidazo[1,2-a]pyrazine compounds, which are coelenterazine analogues, kits comprising the analogues, and methods of using the compounds for the detection of luminescence in luciferase-based assays. Also described are methods from making the compounds, such as a method using aminopyrazine acetophosphonates as synthesis intermediates.
    Type: Application
    Filed: August 29, 2023
    Publication date: February 29, 2024
    Inventors: Anton Shakhmin, Thomas Kirkland, Joel Walker, Thomas Machleidt, Mary Hall, Keith V. Wood
  • Publication number: 20240068010
    Abstract: The present invention disclosure relates to a next generation DNA sequencing method and use for accurate and massively parallel quantification of one or more nucleic acid targets, for example in large volumes of unpurified sample material. More particularly, the invention is related to a method and a kit comprising probes for detecting and quantifying genetic targets in complex samples. The invention includes at least target-specific nucleic acid probes per genetic target (first probe, second probe and target-specific probe) and a bridge oligo or bridge oligo complex.
    Type: Application
    Filed: August 31, 2022
    Publication date: February 29, 2024
    Applicant: Genomill Health Oy
    Inventors: Juha-Pekka Pursiheimo, Tatu Hirvonen, Anttoni Korkiakoski, Manu Tamminen
  • Publication number: 20240068011
    Abstract: A bottleneck in the Next Generation Sequencing (NGS) workflow is the quantification of libraries for accurate pooling and loading of the sequencing instrument flow cell or chip. Disclosed herein are methods that improve performance and reduce time compared to existing methods.
    Type: Application
    Filed: May 23, 2023
    Publication date: February 29, 2024
    Inventors: Vladimir Makarov, Sergey Chupreta
  • Publication number: 20240068012
    Abstract: The invention provides methods, compositions, kits and devices for the detection of target molecules. In some embodiments, the invention allows for multiplexed target molecule detection.
    Type: Application
    Filed: October 12, 2023
    Publication date: February 29, 2024
    Inventor: Garry P. Nolan
  • Publication number: 20240068013
    Abstract: The invention provides methods, compositions, kits and devices for the detection of target molecules. In some embodiments, the invention allows for multiplexed target molecule detection.
    Type: Application
    Filed: October 12, 2023
    Publication date: February 29, 2024
    Inventor: Garry P. Nolan
  • Publication number: 20240068014
    Abstract: A nucleic acid sequence measurement method for measuring a target RNA having a specific nucleic acid sequence included in a cell suspension by hybridization, including a step of heating the cell suspension at 100° C. to 200° C. in a pressurized state to obtain an RNA extract, a step of adding a proteolytic enzyme to the RNA extract to cause the RNA extract to be reacted, thereby preparing a sample solution, a step of supplying the sample solution to a device for nucleic acid sequence measurement, the device being equipped with a fluorescent probe that hybridizes with the target RNA, a step of subjecting the target RNA and the fluorescent probe to a hybridization reaction, and a step of measuring fluorescence from the device for nucleic acid sequence measurement.
    Type: Application
    Filed: January 14, 2022
    Publication date: February 29, 2024
    Inventors: Takashi TADENUMA, Yuki MIYAUCHI, Tomoyuki TAGUCHI
  • Publication number: 20240068015
    Abstract: The invention provides a rapid, sensitive and specific nucleic acid detection system which utilizes isothermal nucleic acid amplification in combination with a lateral flow chromatographic device, or DNA dipstick, for DNA-hybridization detection. The system of the invention requires no complex instrumentation or electronic hardware, and provides a low cost nucleic acid detection system suitable for highly sensitive pathogen detection. Hybridization to single-stranded DNA amplification products using the system of the invention provides a sensitive and specific means by which assays can be multiplexed for the detection of multiple target sequences.
    Type: Application
    Filed: July 6, 2023
    Publication date: February 29, 2024
    Inventors: Hong Cai, Jian Song
  • Publication number: 20240068016
    Abstract: A method for spatially tagging nucleic acids of a biological specimen, including steps of (a) providing a solid support comprising different nucleic acid probes that are randomly located on the solid support, wherein the different nucleic acid probes each includes a barcode sequence that differs from the barcode sequence of other randomly located probes on the solid support; (b) performing a nucleic acid detection reaction on the solid support to locate the barcode sequences on the solid support; (c) contacting a biological specimen with the solid support that has the randomly located probes; (d) hybridizing the randomly located probes to target nucleic acids from portions of the biological specimen; and (e) modifying the randomly located probes that are hybridized to the target nucleic acids, thereby producing modified probes that include the barcode sequences and a target specific modification, thereby spatially tagging the nucleic acids of the biological specimen.
    Type: Application
    Filed: November 9, 2023
    Publication date: February 29, 2024
    Applicants: 10x Genomics Sweden AB, Illumina, Inc.
    Inventors: Jonas Frisen, Patrik Stahl, Joakim Lundeberg, Gordon M. Cann, Leila Bazargan, Alex Aravanis
  • Publication number: 20240068017
    Abstract: Provided herein are methods and kits for determining the optimal conditions for analyte capture in biological samples.
    Type: Application
    Filed: December 28, 2021
    Publication date: February 29, 2024
    Inventors: Joakim Lundeberg, Eva Gracia Villacampa
  • Publication number: 20240068018
    Abstract: A sensor and a method of use may include a structure comprising a plurality of walls that define a plurality of air gaps in the structure, wherein each wall of the plurality of walls may include a plurality of surfaces. The sensor may include a functional layer, wherein the functional layer may be coated on the plurality of walls, wherein the functional layer comprises an extraction component to extract an analyte of interest, at least one amplification initiator to amplify the analyte of interest after extraction, and a material coating the plurality of walls providing an initial surface energy for at least a portion of the plurality of surfaces of the plurality of walls. The initial surface energy of at least the portion of the plurality of surfaces of the plurality of walls provided by the material coating may change when the analyte of interest is present.
    Type: Application
    Filed: August 25, 2023
    Publication date: February 29, 2024
    Inventors: Min Hu, Jacob Timothy Trevino
  • Publication number: 20240068019
    Abstract: The subject invention provides microbe-based products, as well as their use in simultaneously enhancing oil recovery from an oil well while efficiently removing contaminating compositions such as biofilm, scale, paraffin, and/or asphaltenes from oil production equipment and oil-bearing formations. The subject invention can also be used to disperse paraffin and asphaltene precipitates, and to reduce the viscosity of heavy crude oil. The subject invention further provides materials and methods for bioremediation of hydrocarbon-contaminated sites.
    Type: Application
    Filed: October 27, 2023
    Publication date: February 29, 2024
    Inventors: SEAN FARMER, KEN ALIBEK, KENT ADAMS, KARTHIK N. KARATHUR
  • Publication number: 20240068020
    Abstract: Methods are provided herein for identifying rare and/or unknown DNA sequences by next-generation sequencing approaches. Isolated double-stranded (ds), single-stranded (ss), or ds/ss DNA is fragmented and the fragments are polished, phosphorylated, and tailed, as necessary. Fragmentation can be enzymatic or mechanical. A universal adapter sequence is ligated to each fragment, wherein the adapter can have a top strand without a 5? phosphate, a 3? with an —H in place of the —OH, and/or a 3? extra base complementary to any base added to the polished fragments. The ligatamers may then serve as templates for amplification using a forward primer complementary to the adapter sequence and a reverse primer targeted to the fragment sequence. Compositions produced by these methods and kits adapted for performing these methods are also described herein.
    Type: Application
    Filed: September 20, 2023
    Publication date: February 29, 2024
    Inventor: Brian Ward
  • Publication number: 20240068021
    Abstract: Methods, devices, and kits are provided for performing PCR in <20 seconds per cycle, with improved efficiency and yield.
    Type: Application
    Filed: October 25, 2023
    Publication date: February 29, 2024
    Inventors: Carl T. Wittwer, Jared Steven Farrar
  • Publication number: 20240068022
    Abstract: The present invention disclosure relates to a next generation DNA sequencing method and use for accurate and massively parallel quantification of one or more nucleic acid targets, for example in large volumes of unpurified sample material. More particularly, the invention is related to a method and a kit comprising probes for detecting and quantifying genetic targets in complex samples. The invention includes two target-specific nucleic acid probes per genetic target, a barcode loop oligo and a bridge oligo or bridge oligo complex.
    Type: Application
    Filed: August 31, 2022
    Publication date: February 29, 2024
    Applicant: Genomill Health Oy
    Inventors: Juha-Pekka Pursiheimo, Tatu Hirvonen, Anttoni Korkiakoski, Manu Tamminen
  • Publication number: 20240068023
    Abstract: A method for controlling pain in a patient in need thereof includes administering to the patient multiple low doses of methadone, wherein each subsequent dose of methadone is administered within an effective period of time from a previously administered dose of methadone, and wherein the patient has no respiratory depression and no QT prolongation. A method for providing personalized analgesic therapy to a surgical patient includes directing preoperative genotyping of a patient to determine what allele is present at a gene locus to obtain a patient-specific genetic data set, wherein the gene locus is one or more of a locus that encodes an enzyme or protein or transporter or receptor associated with methadone metabolism and responses; producing a prediction of the patient's response to perioperative methadone administration based on the data set; and making a determination whether to administer methadone and a precision perioperative dosing regimen.
    Type: Application
    Filed: January 12, 2022
    Publication date: February 29, 2024
    Inventor: Senthilkumar Sadhasivam