Abstract: The present invention provides a method for the treatment of autosomal dominant Catecholaminergic Polymorphic Ventricular Tachycardia associated with mutations in the cardiac ryanodine receptor type 2 (RYR2) gene, by the use of an AAV mediated RNA interference approach to induce allele specific silencing of mutant mRNA.
Type:
Application
Filed:
February 27, 2023
Publication date:
February 29, 2024
Inventors:
Silvia G. PRIORI, Rossana BONGIANINO, Marco DENEGRI, Carlo NAPOLITANO
Abstract: A method for controlling expression of a nuclear-encoded gene in a plant comprising expressing a chloroplast-encoded dsRNA that silences an endogenous nuclear-encoded gene in the plant to produce a transformed plant line expressing the selected trait.
Abstract: The present invention relates to a wild-type-derived introgression line of a tomato in which the expression of BC2.1 is inhibited and the amount of beta-carotene or lutein is increased; and a transgenic tomato.
Type:
Application
Filed:
December 23, 2021
Publication date:
February 29, 2024
Applicant:
KYUNGPOOK NATIONAL UNIVERSITY INDUSTRY-ACADEMIC COOPERATION FOUNDATION
Abstract: The present disclosure provides composition and methods for improving leaf quality of low-alkaloid tobacco plants. Also provided are the identification and genetic engineering of target genes (Leaf Quality Genes) for producing tobacco plants with altered total alkaloid and nicotine levels and commercially acceptable leaf grade, their development via breeding or transgenic approaches, and production of tobacco products from these tobacco plants.
Abstract: The present invention provides nucleic acids encoding transcription factors and methods of using these nucleic acids to modulate nicotine production in plants and to produce plants having modulated nicotine production.
Abstract: The present disclosure relates to tobacco plants, tobacco seeds, compositions, and methods related to the identification and introgression of the Pale Yellow locus in tobacco. It also relates to generating novel mutations within the PY locus in tobacco.
Type:
Application
Filed:
October 24, 2023
Publication date:
February 29, 2024
Inventors:
Sreepriya PRAMOD, Andrew C. ADAMS, Marcos Fernando DE GODOY LUSSO, Gregory A. DAVIS, Jerry W. MORRIS, Dongmei XU, Jesse FREDERICK
Abstract: Methods and materials for modulating low-nitrogen tolerance levels in plants are disclosed. For example, nucleic acids encoding low nitrogen tolerance-modulating polypeptides are disclosed as well as methods for using such nucleic acids to transform plant cells. Also disclosed are plants having increased low-nitrogen tolerance levels and plant products produced from plants having increased low-nitrogen tolerance levels.
Type:
Application
Filed:
August 14, 2023
Publication date:
February 29, 2024
Inventors:
Gregory Nadzan, Richard Schneeberger, Han Suk Kim, David Van-Dinh Dang, Kenneth A. Feldmann
Abstract: Disclosed are components and methods for RNA-directed DNA cleavage and gene editing. The components include and the methods utilize a Cas9 protein from Neisseria and one or more RNA molecules in order to direct the Cas9 protein to bind to and optionally cleave or nick a target sequence.
Type:
Application
Filed:
November 23, 2022
Publication date:
February 29, 2024
Inventors:
Erik J. Sontheimer, Yan Zhang, Alfonzo Mondragon, Rakhi Rajan, James Thomson, Zhonggang Hou
Abstract: A novel gRNA-ligand binding complex is provided. This complex may be used to bring Type V Cas proteins and additional effectors to DNA for base editing. The design of the systems allows for the production of efficient modular components that provide flexibility when editing DNA.
Type:
Application
Filed:
January 5, 2022
Publication date:
February 29, 2024
Inventors:
Kurt Daniel Marshall, Hide Bueno Machado, Emily Anderson, Alexander Hale, Amanda Smith, Anastasia Kaufman, Leah Nantie, Michael Daniel Rushton, Kevin Hemphill
Abstract: The present disclosure provides isolated promoters, transgene expression cassettes, vectors, kits, and methods for treatment of C9ORF72 associated diseases, including ALS and FTD.
Type:
Application
Filed:
April 24, 2023
Publication date:
February 29, 2024
Inventors:
Peixin Zhu, Xijia Wang, Steven Pennock, Mark Shearman
Abstract: Disclosed herein are chimpanzee adenoviral vectors that include neoantigen-encoding nucleic acid sequences derived from a tumor of a subject. Also disclosed are nucleotides, cells, and methods associated with the vectors including their use as vaccines.
Type:
Application
Filed:
January 10, 2023
Publication date:
February 29, 2024
Inventors:
Wade Blair, Brendan Bulik-Sullivan, Jennifer Busby, Adnan Derti, Leonid Gitlin, Karin Jooss, Ciaran Daniel Scallan, Roman Yelensky, Gijsbert Grotenbreg
Abstract: AAV capsid proteins comprising a modification in the amino acid sequence and virus vectors comprising the modified AAV capsid protein are described. Also described are methods of administering the virus vectors and virus capsids to a cell or to a subject in vivo.
Abstract: The present disclosure provides a variant AAV capsid protein that confers tropism to lung cells and recombinant adeno-associated viruses comprising the variant A.AV and pharmaceutical compositions comprising same and their use in the delivery of heterologous nucleic acids to lung cells for the treatment of pulmonary disorders.
Type:
Application
Filed:
September 15, 2023
Publication date:
February 29, 2024
Applicant:
4D MOLECULAR THERAPEUTICS INC.
Inventors:
Melissa Kotterman, Peter Francis, Melissa Calton, Johnny Gonzales, Roxanne Croze, Christopher Schmitt
Abstract: The present disclosure provides adeno-associated virus (AAV) vectors, comprising coevolved capsid variant proteins, pharmaceutical compositions, methods of making, and methods for delivering such to a subject.
Type:
Application
Filed:
September 26, 2023
Publication date:
February 29, 2024
Applicant:
Duke University
Inventors:
Aravind Asokan, Trevor Gonzalez, Lawrence Patrick Havlik
Abstract: The present invention relates to the prevention and/or treatment of retinal disorders, such as cone dystrophies, cone-rod dystrophies, in particular Achromatopsia.
Type:
Application
Filed:
June 16, 2023
Publication date:
February 29, 2024
Applicant:
UCL BUSINESS LTD
Inventors:
Robin Ali, Takaaki Matsuki, Alexander Smith, Anastasios Georgiadis
Abstract: The present invention relates to polymeric micelle compositions. More specifically, the present invention relates to polymeric micelle compositions for gene delivery.
Abstract: Certain aspects of the present disclosure provides systems, compositions, and methods for regulating expression or activity of an endogenous cytokine of a cell. In some cases, the present disclosure provides a system comprising an actuator moiety capable of complexing with a target gene encoding the endogenous cytokine to regulate expression or activity of the endogenous cytokine. The actuator moiety can be heterologous to the cell. The actuator moiety can be activatable upon exposing the cell to an external stimulus. Upon the exposure of the cell to the external stimulus, the actuator moiety can be activated to regulate expression or activity of the endogenous cytokine.
Type:
Application
Filed:
May 2, 2023
Publication date:
February 29, 2024
Applicant:
FUNDAÇÃO D. ANNA DE SOMMER CHAMPALIMAUD E DR. CARLOS MONTEZ CHAMPALIMAUD FOUNDATION
Inventors:
Maggie L. BOBBIN, Vitaly BALAN, Rona HARARI-STEINFELD, Francesco M. MARINCOLA, Zhifen YANG
Abstract: The present invention provides a method for anaerobic culture of denitrifying microorganisms in culture medium in which nitric acid and/or nitrous acid are added two or more times to the medium during culture, and in which the pH of the medium is maintained within a pH interval by the addition of the nitric acid and/or nitrous acid and the nitric acid and/or nitrous acid also provides the electron acceptor for denitrification in the culture. Methods of producing a cell culture product using such methods and the products which are obtained are also provided.
Type:
Application
Filed:
January 5, 2022
Publication date:
February 29, 2024
Applicant:
NORWEGIAN UNIVERSITY OF LIFE SCIENCES
Inventors:
Lars Reier BAKKEN, Linda BERGAUST, Svein Jarle HORN
Abstract: The disclosure provides a process for the production of ethanol and one or more low boiling compound from a fermentable carbon source. The ethanol and the low boiling compound(s) are produced using an ethanol-producing yeast modified to further produce the one or more low boiling point compounds. In one embodiment, the low boiling compound(s) are acetone, 1-propanol, and/or 2-propanol. Additionally, the disclosure provides a process for the isolation ad purification of the one or more low boiling compounds from ethanol.
Type:
Application
Filed:
August 23, 2023
Publication date:
February 29, 2024
Inventors:
Renata PINTO DA SILVA MATOS, Thiago BEZERRA TAKETA, Felipe GALZERANI, Adler GOMES MOURA
Abstract: A foodstuff can include a free acid ?-hydroxybutyrate, and a base. The ?-hydroxybutyrate, and base, are present at a less than 1:1 molar equivalence.
Abstract: The invention provides a Saccharomyces cerevisiae strain for producing a human milk lipid substitute. By integrating a heterologous lysophosphatidic acid acyltransferase into Saccharomyces cerevisiae and knocking out its own natural lysophosphatidic acid acyltransferase, the content of palmitic acid (C16:0) at Sn-2 position of triacylglycerol produced by Saccharomyces cerevisiae is increased, to synthesize a human milk lipid substitute. On this basis, a metabolic pathway related gene is knocked out, to further increase the content of human milk lipid substitute in the product. In the present invention, a human milk lipid substitute is de novo synthesized by Saccharomyces cerevisiae for the first time, in which the total fatty acid is 15% or more, and the relative content of C16:0 at Sn-2 position reaches about 60%.
Type:
Application
Filed:
November 1, 2023
Publication date:
February 29, 2024
Inventors:
Long LIU, Jian Chen, Xueqin LV, Wenyang Wu, Guocheng Du, Jianghua Li, Guolin Zhou, Yanfeng Liu, Chenyang Zhang
Abstract: The disclosure relates to host cells having altered NADPH availability, allowing for increased production of compounds produced using NADPH, and methods of use thereof. NADPH availability is altered by one or more of: expressing an altered GAPDH, expressing a variant glutamate dehydrogenase (gdh), aspartate semialdehyde dehydrogenase (asd), dihydropicolinate reductase (dapB), and meso-diaminopimelate dehydrogenase (ddh), expressing a novel nicotinamide nucleotide transhydrogenase, expressing a novel threonine aldolase, and expressing or modulating the expression of a pyruvate carboxylase in the host cells.
Type:
Application
Filed:
October 7, 2022
Publication date:
February 29, 2024
Inventors:
Shawn MANCHESTER, Benjamin MASON, Alexi GORANOV
Abstract: Disclosed are strain having enhanced L-glutamic acid production capacity, and method for constructing the same and use thereof. A nucleotide sequence is provided by introducing a point mutation to a wild-type BBD29-00405 gene in Corynebacterium glutamicum so that the base at position 597 of SEQ ID NO: 1 is mutated from guanine (G) into adenine (A). Also provided is a recombinant strain obtained by introducing the polynucleotide sequence into L-glutamic acid-producing Corynebacterium glutamicum, the recombinant strain comprising a BBD29-00405 gene containing a point mutation. Compared with an unmodified strain, the resulting strain facilitates production of L-glutamic acid at a higher concentration.
Type:
Application
Filed:
December 29, 2021
Publication date:
February 29, 2024
Applicant:
NINGXIA EPPEN BIOTECH CO., LTD
Inventors:
Houbo SU, Aiying WEI, Gang MENG, Lipeng YANG, Fengyong MA, Huiping JIA, Xiaoqun ZHOU, Chunguang ZHAO
Abstract: A recombinant strain with modified gene BBD29_11265 and a method for constructing the same are provided. The recombinant strain is a bacterium that generates L-glutamic acid, and has an improved expression of a polynucleotide encoding an amino acid sequence of SEQ ID NO: 3 or a homologous sequence thereof; the improved expression can be having a point mutation in, and an enhanced expression of the polynucleotide encoding an amino acid sequence of SEQ ID NO: 3 or a homologous sequence thereof. A genetically engineered bacterium in which the base at position 70 in the BBD29_112665 gene sequence is mutated to adenine from guanine, causing alanine at position 24 in the coded corresponding amino acid sequence to be substituted with threonine, and an engineered bacterium overexpressing the BBD29_112665 gene or BBD29_11265G70A gene are constructed in the present invention, facilitating an increase in the production and conversion rate of L-glutamic acid.
Type:
Application
Filed:
December 28, 2021
Publication date:
February 29, 2024
Applicant:
NINGXIA EPPEN BIOTECH CO., LTD
Inventors:
Aiying WEI, Gang MENG, Chunguang ZHAO, Huiping JIA, Houbo SU, Lipeng YANG, Xiaowei GUO, Bin TIAN, Fengyong MA, Xiaoqun ZHOU
Abstract: The present invention relates to an E. coli hisG-derived ATP-phosphoribosyltransferase variant having a reduced feedback inhibition by histidine and a strain expressing the same. The variant may maintain its activity even at a high histidine concentration, thus increasing histidine production.
Type:
Application
Filed:
April 26, 2021
Publication date:
February 29, 2024
Applicant:
DAESANG CORPORATION
Inventors:
Jong Yun HAN, Chel Min YANG, Yong Soo KIM, Young Il JO
Abstract: The disclosure discloses a method for chemically synthesizing a Helicobacter pylori core lipopolysaccharide oligosaccharide antigen carbohydrate chain, and belongs to the technical field of carbohydrate chemistry. The disclosure uses D-glucose, D-galactose and D-mannose as starting materials, which undergo a series of protection and deprotection reactions to prepare eight monosaccharide blocks. The eight monosaccharide block compounds as shown in formulas 2 to 9 undergo glycosylation reactions under catalysis of corresponding activating reagents, to prepare H. pylori lipopolysaccharide core oligosaccharide antigen fragments as shown in formula 1. The disclosure further combines the synthesized oligosaccharide fragments with a chip to make a carbohydrate chip, screens the optimal antigen fragments using patient serum, or combines the synthesized oligosaccharide fragments with carrier proteins to make glycoconjugates.
Abstract: The present invention relates to a recombinant microorganism comprising one or more nucleotide sequence(s) encoding: a polypeptide having ent-copalyl pyrophosphate synthase activity; a polypeptide having ent-Kaurene synthase activity; a polypeptide having ent-Kaurene oxidase activity; and a polypeptide having kaurenoic acid 13-hydroxylase activity, whereby expression of the nucleotide sequence(s) confer(s) on the microorganism the ability to produce at least steviol.
Abstract: Described herein are engineered organelles comprising multi-component proteins from different species incorporated into a membrane structure with interior and exterior aspects. In one embodiment the artificial organelle incorporates one or more protein complexes that absorb optical energy and catalyze electron transfer in biochemical reactions that can be used to reduce NAD+ to NADH or analogues thereof.
Type:
Application
Filed:
September 21, 2023
Publication date:
February 29, 2024
Inventors:
Kyle A. Minor, Carlo D. Montemagno, David W. Wendell
Abstract: Provided herein are methods and cell-free systems for production of a recombinant antibody in which the antibody is directed against a target protein that is natively expressed as an intracellular protein. Also provided herein are modified cell-free systems for use in such methods in which the cell free protein expression system is modified by an agent which binds to the intracellular target protein or to the antibody paratope and thus prevents the antibody from binding to the target protein.
Type:
Application
Filed:
May 2, 2023
Publication date:
February 29, 2024
Applicant:
MBrace Therapeutics, Inc.
Inventors:
Yongjian Wu, Fernanda I. Staquicini, Isan Chen
Abstract: The present invention provides engineered ketoreductase and phosphite dehydrogenase enzymes having improved properties as compared to a naturally occurring wild-type ketoreductase and phosphite dehydrogenase enzymes, as well as polynucleotides encoding the engineered ketoreductase and phosphite dehydrogenase enzymes, host cells capable of expressing the engineered ketoreductase and phosphite dehydrogenase enzymes, and methods of using the engineered ketoreductase and phosphite dehydrogenase enzymes to synthesize a chiral catalyst used in the synthesis of antiviral compounds, such as nucleoside inhibitors. The present invention further provides methods of using the engineered enzymes to deracemize a chiral alcohol in a one-pot, multi-enzyme system.
Type:
Application
Filed:
July 14, 2023
Publication date:
February 29, 2024
Inventors:
Jeffrey C. Moore, Jack Liang, Jonathan Penfield, Jovana Nazor, Nikki Dellas, Vesna Mitchell, Da Duan, Iman Farasat, Agustina Rodriguez-Granillo, Grant Murphy, Nicholas Marshall
Abstract: A method for assessing drug-resistant Klebsiella pneumoniae includes the following steps. A test sample is provided, wherein the test sample includes a Klebsiella pneumoniae. A spectrum analysis step is performed, wherein the test sample is detected by a mass spectrometry method so as to obtain a target mass spectrum data. An assessing step for drug-resistant Klebsiella pneumoniae is performed, wherein the target mass spectrum data is analyzed so as to assess whether the Klebsiella pneumoniae is resistant to a carbapenem antibiotic or a colistin or not. When the Klebsiella pneumoniae is resistant to the carbapenem antibiotic, the target mass spectrum data includes a first anti-carbapenem feature mark, and when the Klebsiella pneumoniae is resistant to the colistin, the target mass spectrum data includes a first anti-colistin feature mark.
Type:
Application
Filed:
January 16, 2023
Publication date:
February 29, 2024
Applicant:
China Medical University
Inventors:
Der-Yang Cho, Po-Ren Hsueh, Jiaxin Yu, Ni Tien, Hsiu Hsien Lin, Chia-Fong Cho
Abstract: Provided herein are biomarkers associated with risk of bacterial infection, particularly hospital inquired infections, such as following surgeries. In particular, provided herein are fecal biomarkers (e.g., microbiomic, metabolomic, etc.) that correlate with elevated risk of infection in subjects following surgery (e.g., organ transplant surgery), and methods of treating and/or preventing such infections.
Type:
Application
Filed:
August 21, 2023
Publication date:
February 29, 2024
Inventors:
Eric Gerd Pamer, Nicholas Dylla, Christopher Lehmann
Abstract: The present invention relates generally to materials and methods for detection of bacteria, and for testing and determination of antibiotic susceptibility of bacteria in specimens of bodily fluid and other samples. The invention also relates to materials and methods for monitoring the physiological response of bacteria to antimicrobial agents, and for reducing background and increasing sensitivity of assays that involve the detection and/or measurement of RNA, such as rRNA. The invention provides kits comprising an RNase packaged for use in the methods described herein.
Type:
Application
Filed:
August 21, 2023
Publication date:
February 29, 2024
Inventors:
David A. Haake, Daniel Gussin, Gabriel K. Monti, Bernard M. Churchill, Colin W. Halford
Abstract: Described are substituted imidazo[1,2-a]pyrazine compounds, which are coelenterazine analogues, kits comprising the analogues, and methods of using the compounds for the detection of luminescence in luciferase-based assays. Also described are methods from making the compounds, such as a method using aminopyrazine acetophosphonates as synthesis intermediates.
Type:
Application
Filed:
August 29, 2023
Publication date:
February 29, 2024
Inventors:
Anton Shakhmin, Thomas Kirkland, Joel Walker, Thomas Machleidt, Mary Hall, Keith V. Wood
Abstract: The present invention disclosure relates to a next generation DNA sequencing method and use for accurate and massively parallel quantification of one or more nucleic acid targets, for example in large volumes of unpurified sample material. More particularly, the invention is related to a method and a kit comprising probes for detecting and quantifying genetic targets in complex samples. The invention includes at least target-specific nucleic acid probes per genetic target (first probe, second probe and target-specific probe) and a bridge oligo or bridge oligo complex.
Type:
Application
Filed:
August 31, 2022
Publication date:
February 29, 2024
Applicant:
Genomill Health Oy
Inventors:
Juha-Pekka Pursiheimo, Tatu Hirvonen, Anttoni Korkiakoski, Manu Tamminen
Abstract: A bottleneck in the Next Generation Sequencing (NGS) workflow is the quantification of libraries for accurate pooling and loading of the sequencing instrument flow cell or chip. Disclosed herein are methods that improve performance and reduce time compared to existing methods.
Abstract: The invention provides methods, compositions, kits and devices for the detection of target molecules. In some embodiments, the invention allows for multiplexed target molecule detection.
Abstract: The invention provides methods, compositions, kits and devices for the detection of target molecules. In some embodiments, the invention allows for multiplexed target molecule detection.
Abstract: A nucleic acid sequence measurement method for measuring a target RNA having a specific nucleic acid sequence included in a cell suspension by hybridization, including a step of heating the cell suspension at 100° C. to 200° C. in a pressurized state to obtain an RNA extract, a step of adding a proteolytic enzyme to the RNA extract to cause the RNA extract to be reacted, thereby preparing a sample solution, a step of supplying the sample solution to a device for nucleic acid sequence measurement, the device being equipped with a fluorescent probe that hybridizes with the target RNA, a step of subjecting the target RNA and the fluorescent probe to a hybridization reaction, and a step of measuring fluorescence from the device for nucleic acid sequence measurement.
Abstract: The invention provides a rapid, sensitive and specific nucleic acid detection system which utilizes isothermal nucleic acid amplification in combination with a lateral flow chromatographic device, or DNA dipstick, for DNA-hybridization detection. The system of the invention requires no complex instrumentation or electronic hardware, and provides a low cost nucleic acid detection system suitable for highly sensitive pathogen detection. Hybridization to single-stranded DNA amplification products using the system of the invention provides a sensitive and specific means by which assays can be multiplexed for the detection of multiple target sequences.
Abstract: A method for spatially tagging nucleic acids of a biological specimen, including steps of (a) providing a solid support comprising different nucleic acid probes that are randomly located on the solid support, wherein the different nucleic acid probes each includes a barcode sequence that differs from the barcode sequence of other randomly located probes on the solid support; (b) performing a nucleic acid detection reaction on the solid support to locate the barcode sequences on the solid support; (c) contacting a biological specimen with the solid support that has the randomly located probes; (d) hybridizing the randomly located probes to target nucleic acids from portions of the biological specimen; and (e) modifying the randomly located probes that are hybridized to the target nucleic acids, thereby producing modified probes that include the barcode sequences and a target specific modification, thereby spatially tagging the nucleic acids of the biological specimen.
Type:
Application
Filed:
November 9, 2023
Publication date:
February 29, 2024
Applicants:
10x Genomics Sweden AB, Illumina, Inc.
Inventors:
Jonas Frisen, Patrik Stahl, Joakim Lundeberg, Gordon M. Cann, Leila Bazargan, Alex Aravanis
Abstract: A sensor and a method of use may include a structure comprising a plurality of walls that define a plurality of air gaps in the structure, wherein each wall of the plurality of walls may include a plurality of surfaces. The sensor may include a functional layer, wherein the functional layer may be coated on the plurality of walls, wherein the functional layer comprises an extraction component to extract an analyte of interest, at least one amplification initiator to amplify the analyte of interest after extraction, and a material coating the plurality of walls providing an initial surface energy for at least a portion of the plurality of surfaces of the plurality of walls. The initial surface energy of at least the portion of the plurality of surfaces of the plurality of walls provided by the material coating may change when the analyte of interest is present.
Abstract: The subject invention provides microbe-based products, as well as their use in simultaneously enhancing oil recovery from an oil well while efficiently removing contaminating compositions such as biofilm, scale, paraffin, and/or asphaltenes from oil production equipment and oil-bearing formations. The subject invention can also be used to disperse paraffin and asphaltene precipitates, and to reduce the viscosity of heavy crude oil. The subject invention further provides materials and methods for bioremediation of hydrocarbon-contaminated sites.
Type:
Application
Filed:
October 27, 2023
Publication date:
February 29, 2024
Inventors:
SEAN FARMER, KEN ALIBEK, KENT ADAMS, KARTHIK N. KARATHUR
Abstract: Methods are provided herein for identifying rare and/or unknown DNA sequences by next-generation sequencing approaches. Isolated double-stranded (ds), single-stranded (ss), or ds/ss DNA is fragmented and the fragments are polished, phosphorylated, and tailed, as necessary. Fragmentation can be enzymatic or mechanical. A universal adapter sequence is ligated to each fragment, wherein the adapter can have a top strand without a 5? phosphate, a 3? with an —H in place of the —OH, and/or a 3? extra base complementary to any base added to the polished fragments. The ligatamers may then serve as templates for amplification using a forward primer complementary to the adapter sequence and a reverse primer targeted to the fragment sequence. Compositions produced by these methods and kits adapted for performing these methods are also described herein.
Abstract: The present invention disclosure relates to a next generation DNA sequencing method and use for accurate and massively parallel quantification of one or more nucleic acid targets, for example in large volumes of unpurified sample material. More particularly, the invention is related to a method and a kit comprising probes for detecting and quantifying genetic targets in complex samples. The invention includes two target-specific nucleic acid probes per genetic target, a barcode loop oligo and a bridge oligo or bridge oligo complex.
Type:
Application
Filed:
August 31, 2022
Publication date:
February 29, 2024
Applicant:
Genomill Health Oy
Inventors:
Juha-Pekka Pursiheimo, Tatu Hirvonen, Anttoni Korkiakoski, Manu Tamminen
Abstract: A method for controlling pain in a patient in need thereof includes administering to the patient multiple low doses of methadone, wherein each subsequent dose of methadone is administered within an effective period of time from a previously administered dose of methadone, and wherein the patient has no respiratory depression and no QT prolongation. A method for providing personalized analgesic therapy to a surgical patient includes directing preoperative genotyping of a patient to determine what allele is present at a gene locus to obtain a patient-specific genetic data set, wherein the gene locus is one or more of a locus that encodes an enzyme or protein or transporter or receptor associated with methadone metabolism and responses; producing a prediction of the patient's response to perioperative methadone administration based on the data set; and making a determination whether to administer methadone and a precision perioperative dosing regimen.