Abstract: The disclosed embodiments include a rock sample inspection method. The method may include preparing a sample of formation rock by encapsulating the sample, inserting the sample into a vessel body as part of a test assembly, enclosing the sample within an low compressibility fluid, applying pressure to an interior of the vessel body by tightening a compression screw employing a piston acting on said low compressibility fluid, monitoring the pressure, conducting a test on the sample, and recording results of the test for further analysis.

Abstract: An inspection apparatus for inspecting an inspection target object, includes an X-ray generation tube having a target including an X ray generation portion that generates X-rays by irradiation with an electron beam, and configured to emit X-rays to an inspection target surface of the inspection target object, an X-ray detector configured to detect X-rays emitted from a foreign substance existing on the inspection target surface irradiated with the X-rays from the X ray generation portion and totally reflected by the inspection target surface, and an adjustment mechanism configured to adjust a relative position between the inspection target surface and the X-ray detector.

Abstract: A sample inspection system contains a source of electromagnetic radiation and an apparatus that includes a beam former, a collimator and an energy resolving detector. The beam former is adapted to receive electromagnetic radiation from the source to provide a polygonal shell beam formed of at least three walls of electromagnetic radiation. The collimator has a plurality of channels adapted to receive diffracted or scattered radiation at an angle. The energy resolving detector is arranged to detect radiation diffracted or scattered by a sample upon incidence of the polygonal shell beam onto the sample and transmitted by the collimator.

Abstract: The invention relates to a method of examining a sample using a charged particle microscope, comprising the steps of providing a charged particle beam, as well as a sample; scanning said charged particle beam over said sample at a plurality of sample locations; and detecting, using a first detector, emissions of a first type from the sample in response to the beam scanned over the plurality of sample locations. Spectral information of detected emissions of the first type is used to assign a plurality of mutually different phases to said sample at said plurality of sample locations. Information relating to at least one previously assigned phase and its respective sample location is used for establishing an estimated phase for at least one other of the plurality of sample locations. Said estimated phase is assigned to said other sample location. A control unit is used to provide a data representation of said sample containing at least information on said plurality of sample locations and said phases.

Abstract: This disclosure relates to monitoring and assessing the mechanical stability and fluid accumulation in natural or man-made slopes comprising primarily of unconsolidated material, such as embankments, dams, roads, waste dumps, as well as man-made heaps of bulk materials that may occur in the stockpiling of grains, gravel, stones, sand, coal, cement, fly ash, salts, chemicals, clays, crushed limestone as well as heaps of mining ores, including crushed, milled and/or agglomerated ore, and run-of-mine materials.

Abstract: A method of using the transverse relaxation time (T2) of solvent NMR signal to detect filling errors of an alum-containing product in real-time and in-line during manufacturing, for example during a fill-finish unit operation. This method can be used for quality control in vaccine manufacturing to ensure the delivery of the correct concentration of alum-containing product to the product container such as a vial or pre-filled syringe.

Abstract: A method for detecting adsorption performance of microplastics for heavy metals using low-field NMR relaxation method, including: establishing a reference line reflecting a relationship between a target heavy metal ion concentration and a transverse relaxation time; conducting a heavy metal adsorption experiment on microplastics and obtaining samples at different time points; detecting transverse relaxation times of the multiple samples obtained; determining a heavy metal ion concentration of each sample based on the reference line of the target heavy metal ion concentration and the transverse relaxation time; analyzing data and implementing a model fitting process. The method according to the present disclosure does not require cumbersome and complicated sample pre-processing, it can be measured immediately after sampling, it is simple, fast and low-cost, the method has a wide detection limit, the detection is faster, non-destructive testing can be achieved.

Abstract: An experimental technology for detecting a hydrogen bond based on an ssNMR technology includes: (1) exciting a 1H nucleus of an RNA sample with a ?/2 pulse; (2) applying two ? pulses every half rotation period on an X nucleus of the RNA sample; (3) applying a ? pulse on the 1H nucleus of the RNA sample; (4) applying two ? pulses every half rotation period on the X nucleus of the RNA sample; (5) applying a 90° pulse on 1H and X atoms of the RNA sample; r a chemical shift of X in indirect dimension; (7) applying the 90° pulse on the 1H and X nuclei of the RNA sample; (8) repeating steps 2, 3, and 4; and (9) collecting the 1H signal in direct dimension; where X is selected from the group consisting of 15N and 13C.

Abstract: The present invention describes a versatile, robust and environmentally controlled platform with a linear temperature gradient for massively parallel chemical or biochemical processing. This apparatus is capable of probing the phase transition behavior of macromolecules in solution, both thermodynamically and kinetically. This includes—but is not limited to—liquid/liquid phase transition behavior of antibody solutions and in situ gelation of thermo-responsive polymers. The device can be operated in a multiplex fashion using a controlled temperature gradient architecture and visualized by dark field microscopy or by other optical intensity measurements.

Abstract: The thermal sensor chip includes a substrate in which a cavity having an opening is formed, a membrane provided on a surface of the substrate so as to cover the opening, and a heater provided on or inside the membrane, wherein the heater includes wires in a mesh form constituted by a conductive member.

Abstract: The invention relates to a method for producing a fiber-plastic composite reference body for simulating delamination for the nondestructive testing of FPC components, in particular aircraft components, comprising the following steps: i. producing a first insert by a. arranging a first FPC layer; b. forming a recess in the first FPC layer; c. procuring the first FPC layer, in order to obtain the first insert; ii. producing a second insert by a. arranging a second FPC layer; b. pre-curing the second FPC layer, in order to obtain the second insert; iii. providing at least one first FPC layer and at least one second FPC layer with a first clearance and a second clearance; iv. inserting the first insert and the second insert into the respective clearance of the corresponding FPC layer; v. curing the arrangement, a delamination being simulated at the recess of the first insert.

Abstract: A pump device has at least one chamber (22) or conduit containing or provided for containing a liquid, a concentration sensor (24) arranged in the chamber (22) or conduit for detecting a concentration of a substance in the liquid and an evaluation unit (28) connected to the sensor (24). The sensor (24) and the evaluation unit (28) are configured for an electrical impedance measurement. The evaluation unit (28) is configured such that a measurement for detecting the concentration is carried out by use of an electrical signal applied to the sensor (24) having at least one frequency corresponding to or above an upper cut-off frequency (f2) of a frequency range showing a constant electrical impedance (Rm). A method is provided for determining the concentration of a substance inside a liquid.

Abstract: Provided is a gas detection device used in a humid environment such as a kitchen or a cooking room, which is excellent in moisture resistance and also excellent in sensitivity. In a gas detection device which includes a thin film type gas sensor including a heater portion, a gas detection portion, and a catalyst portion on a substrate, energizes the heater portion to heat the gas detection portion and the catalyst portion, and detects the detection target gas, the gas sensor configured by supporting a catalyst metal containing platinum as a main component on a support containing a transition metal oxide as a main component is adopted.

Abstract: System for measuring the composition of a multiphase fluid flow in a pipe, the multiphase flow containing a mixture of gas and liquid. The system comprising a first and a second sensor arrangement, wherein each sensor arrangement is adapted to measuring electrical characteristics (i.e. impedance) of the flow at a predetermined rate. The system also including an analysis unit being adapted to monitor the measured electrical characteristics from said first and second sensor arrangement and to detect occurrences of time periods involving a predetermined relationship between the measured characteristics, and during these periods assuming a liquid rich phase and calculating the composition based on the electrical characteristics.

Abstract: The invention relates to a method of patterning a substrate with graphene-based or other electroactive-material-based solution that includes solid-phase particles as hard templates, reducing the solution, and processing the reduced solution to expose the particles. The exposed hard template particles are removed to leave a three-dimensional (3D) porous architecture that can be beneficially used for a variety of applications, including but not limited to bio sensors and supercapacitors. In one example, the exposure is by etching with a CO2 laser. The method can be practiced with scalable MEMS fabrication technologies.

Abstract: Electrophoresis is used to identify presence of a target compound in a patient sample based on a charge state of the compound and a label. The charge state of the compound correlates to a total net charge of a binder conjugated to the compound. The bound complex or “bound complex” with the label is then applied to the electrophoresis substrate. An electric potential is applied to the substrate for a time period and causes the labeled bound complex to migrate toward the electrode with opposite charge of the labeled bound complex at a migration velocity to form a migration pattern over the time period. At some time during or at the end of the time period, the labeled bound complex produces a bound complex band as a result of its migration across the substrate. The presence of the compound is identified based on the labeled bound complex band and one or both of the migration pattern and the migration velocity.

Abstract: A cyclic capillary electrophoresis device includes a capillary channel that forms a closed loop. The capillary channel comprises an inner half facing toward a space enclosed by the loop, where the inner half having an inner wall of first charge density, and an outer half facing away from the space enclosed by the loop, where the outer half having an inner wall surface of second charge density. A difference between the first and the second charge densities exists or can be turned on. The difference is configured to create a smaller average electroosmotic flow velocity in the inner half than in the outer half.

Abstract: A monitoring system detects and measures a quantity of radical particles within a gas. A test chamber is coupled to a flow channel that transmits a gas. The test chamber defines an aperture connecting the test chamber and the flow channel, and the aperture permits a subset of the gas to enter the test chamber from the flow channel. An ionizer is positioned within the test chamber and generates radical ions from radical particles of the subset of the gas. A mass spectrometer measures a quantity of the radical ions, thereby providing a measurement of the radical particles in the gas.

Abstract: The subject disclosure presents systems and computer-implemented methods for determining an acoustic time-of-flight (TOF) of sound waves through a sample material with greater accuracy and in a more repeatable fashion, by invoking one or more of an envelope generation for an error function, fitting a non-linear curve to an ultrasound frequency sweep, or performing a clustered piece-wise linear regression on individual linear parts of the ultrasonic frequency sweep. The systems and methods are useful for, among other things, monitoring diffusion of fluids through porous materials, such as tissue samples.

Abstract: A test device for a destruction-free test of rotationally symmetrical objects, in particular rotors of gas or steam turbines. The test device has at least three elongated skid bodies which extend in the circumferential direction and parallel to one another, which are interconnected, and which define contact surfaces for positioning on the circumferential surface of the object to be tested, wherein a test body receiving area for an ultrasonic test probe is provided at least on the center skid body.

Abstract: Systems, methods, and apparatus for ultra-sonic inspection of a surface are described. An example system may include an inspection robot structured to move in a direction of travel on an inspection surface. The inspection robot may include a payload including a first ultrasonic (UT) phased array and a second UT phased array, the first UT phased array and the second UT phased array being arranged in a parallel configuration. The inspection robot may include a rastering device structured to move the payload in a direction of inspection, the direction of inspection being distinct from the direction of travel and the direction of inspection being distinct from the parallel configuration of the first UT phased array and the second UT phased array.

Abstract: A method includes two or more reference strain gradient information being a relationship between the strain at the hole edge and a strain gradient along a radial direction. Hole expansion forming is performed on the evaluation metal sheet under the same forming conditions as respective forming conditions corresponding to at least two pieces of the reference strain gradient information to obtain at least two limit hole expansion ratios at a hole expansion limit of the evaluation metal sheet. A formable region of the evaluation metal sheet is obtained from the at least two pieces of the reference strain gradient information and the obtained at least two limit hole expansion ratios at the hole expansion limit. Stretch flange cracking at the sheared end face of the evaluation metal sheet is evaluated by the obtained formable region.

Abstract: An in-situ bollard tester. The in-situ bollard tester may comprise: a frame, cable, and tensioner. The frame is preferably adapted to mount onto a pier or wharf and around a bollard to provide structural support for the cable and tensioner. The frame may comprise a rectangular frame, pair of hanging columns, pair of jacks and pair of legs. The pair of jacks are coupled near proximal corners of the rectangular frame and are vertically disposed. The hanging columns are coupled near distal corners of the rectangular frame. The pair of legs are coupled at the lower ends of the hanging columns and are disposed in a horizontal manner. The tensioner may be coupled above the rectangular frame. The cable may fasten to the bollard, and the tensioner may apply tension to the cable at various load angles in order to test the integrity of the bollard.

Abstract: The present disclosure describes a field flow fractionator (FFF). In an embodiment, the FFF includes a spacer including a core, and a coating covering the core. In an embodiment, the FFF includes a spacer including a core, and a coating covering the core, a bottom block including pins, and a top block including mating holes to receive the pins, where the spacer is configured to be positioned between the top block and the bottom block. In an embodiment, the FFF includes a spacer including a core including an opening, a top side, and a bottom side, a first coating covering the top side, and a second coating covering the bottom side, a top block configured to press on the first coating along a first periphery of the opening, and a bottom block configured to press on the second coating along a second periphery of the opening.

Abstract: Embodiments of the present disclosure may assist in managing, storing, using, and re-using dedicated chromatography column bed supports. For example, devices and systems disclosed herein may include reusable cases for storing dedicated column bed supports when the column bed supports are not in use. Such cases may include one or more dividers and inserts configured to fit column bed supports, and may be designed and built specifically to ensure efficient, secure storage of column bed supports while minimizing risk of damaging or contaminating them. Methods disclosed herein may include performing a particular chromatography operation in a column, removing the column bed supports from the column, storing the column bed supports in a column bed support storage case, and installing another set of column bed supports in the column for another chromatography operation.

Abstract: The electron capture detector (100) is a device for detecting a sample (?1). The electron capture detector (100) includes a detection cell (1), a sample inlet (2), and an electron emitting element (20). The detection cell (1) forms a reaction chamber (6). The sample inlet (2) introduces a first carrier gas containing the sample (?1) into the reaction chamber (6). The electron emitting element (20) emits electrons (?) into the reaction chamber (6). An ion (?2) derived from the sample component is generated as a result of the electron emitting element (20) emitting electrons (?) into the reaction chamber (6).

Abstract: A breath alcohol device calibration system includes a computerized calibration module operable to calibrate a breath alcohol device, and an interface operable to couple the breath alcohol device to a remote server. The interface uses a connection employing a cryptographic function such that data stored on the breath alcohol device can be securely transferred from the breath alcohol device to the remote server using the calibration system. The interface is further operable to transfer data stored on the breath alcohol device from the breath alcohol device to the remote server directly without storing the data in nonvolatile storage on the calibration station.

Abstract: A system and method for sampling air at multiple locations in a controlled environment. The system and method includes automatic adjustment of mass flow rates and duration of vacuum connections (either via time elapsed or indirectly by volume) based on rates set by an operator. Additionally, the system and method enables users to monitor and control aspects of the system via network-connected devices. Additionally, the system enable a vacuum pump to be disconnected from power in response to a physical emergency button, a software-based emergency stop button available on network connected devices, and an automatic power disconnection in response to an abnormal mass flow reading that could potentially impact the vacuum pump.

Abstract: A gas sensing device includes one or more chemo-resistive gas sensors; one or more heat sources, wherein the gas sensors are heated according to one or more first temperature profiles during the recovery phases and according to one or more second temperature profiles during the sense phases; a preprocessing processor for generating preprocessed signal samples; a feature extraction processor for extracting one or more feature values from the received preprocessed signal samples; and a gas concentration processor for creating a sensing result, wherein the gas concentration processor includes a classification processor for outputting a class decision value, wherein the classification processor is configured for outputting a confidence value, wherein the classification processor includes a first trained model based algorithm processor, wherein the gas concentration processor comprises a quantification processor for creating an estimation value, and wherein the quantification processor comprises a second trained m

Abstract: Methods, apparatuses, and kits for detection of substances in water by forming a complex with an indicator reagent and filtering the complex on a filter or membrane are described. The concentrated indicator on the filter, membrane or other capture device provides a colorimetric readout that can determine the amount of substance present in a water sample. A computer-implemented method for determining the concentration of substances in water based on color data from the colorimetric readout is also described.

Abstract: A resonant sensor probe assembly includes a substrate formed from one or more dielectric materials and free-standing electrodes coupled with the substrate. The free-standing electrodes are configured to be placed into the fluid and to generate an electric field between the free-standing electrodes. A controller measures an impedance response of the sensor to the fluid between the electrodes to determine an aging effect of the sensor.

Abstract: The method includes the steps of performing in-vitro liver, intestinal and/or expressed enzyme assays with selected ethnobotanical substances, for both humans and a variety of animal species, to produce an array of resulting chemical entities, such as metabolites, for the human and the animals. Comparisons are then made between the chemical entities from the human in-vitro studies and the animal in-vitro studies to determine the closest match. The animal with the closest match is then used for an in-vivo study. If a match is present between the animal in-vivo results and the human in-vitro results, the matched chemical entity is isolated or synthesized and then further tested to determine the suitability of the matched chemical entity as a treatment drug.

Abstract: The present invention relates to methods and kits for prognosing, treating, and managing treatment of cancer in a subject. The methods involve selecting a subject having cancer and obtaining, from the selected subject, a sample containing exosomes or an S100 molecule containing sample. The exosomes or S100 molecule containing sample, respectively, are then contacted with one or more reagents suitable to detect higher or lower levels or the presence or absence of one or more integrins on said exosomes or higher or lower levels or the presence or absence of one or more S100 molecules in the S100 molecule containing sample. The cancer is then prognosed, treatment is administered, or treatment is managed.

Abstract: A time-resolved fluorescence kit for synchronously detecting 4,15-diacetoxyscirpenol, aflatoxin B1 and sterigmatocystin includes an immunochromatography time-resolved fluorescence test strip and a sample reaction bottle containing europium-labeled anti-4,15-diacetoxyscirpenol, anti-aflatoxin B1 and anti-sterigmatocystin monoclonal antibodies, where a water absorption pad, a detection pad and a sample pad are sequentially disposed on one side of the immunochromatography time-resolved fluorescence test strip from top to bottom, adjacent pads are connected in an overlapping manner at a joint, the detection pad uses a nitrocellulose membrane as a base pad, a quality control line and detection lines are transversely arranged on the nitrocellulose membrane from top to bottom, the quality control line is coated with a rabbit antimouse polyclonal antibody, and the three detection lines each are coated with a toxin-protein conjugate.

Abstract: The present disclosure provides a system comprising a communication interface and computer for assigning a label to the biomolecule fingerprint, wherein the label corresponds to a biological state. The present disclosure also provides a sensor arrays for detecting biomolecules and methods of use. In some embodiments, the sensor arrays are capable of determining a disease state in a subject.

Abstract: In one aspect, molecular sensors and methods of making molecular sensors are described herein. In some embodiments, such a sensor comprises a first layer having a dual nanohole structure and a second layer having at least one nanopore. In some embodiments, the first and second layer define a chip of the sensor. In another aspect, methods of sensing are described herein, which in some embodiments comprise (i) providing a test sample comprising complexed and/or non-complexed biomolecules; (ii) contacting the test sample with the first layer of the molecular sensor; (iii) irradiating the dual nanohole structure of the sensor with a beam of electromagnetic radiation; (iv) optically trapping the biomolecules in the dual nanohole structure and measuring a surface plasmon resonance; (v) applying an electric field across the nanopore of the sensor; and (vi) measuring change in current across the nanopore during one or more translocation events of the biomolecules.

Abstract: Systems, methods, and devices for selective capture and release of target particles, e.g., living cells, from liquid samples, e.g., blood, are provided. The particle capture systems include a substrate; a first layer of gelatin bound to the substrate by physical adsorption, wherein the gelatin is functionalized with a plurality of first members of a binding pair; a second layer of gelatin wherein the gelatin is functionalized with a plurality of the first members of the binding pair and the second layer is bound to the first layer via a plurality of second members of the binding pair that are associated with the first members of the binding pair on both the first and the second layers; and a plurality of nanostructures bound to the second members of the binding pair and to one or more particle-binding moieties that selectively bind to the target particles.

Abstract: An in vitro method is disclosed for detecting an antibody to p53 (anti-p53 antibody) in a sample, the method comprising: incubating a sample to be analyzed with a p53 capture antigen and a p53 detection antigen, whereby a complex comprising the p53 capture antigen, the anti-p53 antibody and the p53 detection antigen is formed, separating the complex formed from unbound detection antigen and measuring the complex obtained via the detection antigen comprised therein, thereby detecting the anti-p53 antibody comprised in the sample.

Abstract: The present invention, in some embodiments thereof, generally relates to methods and devices for determining the health status of a subject, e.g., whether the subject has a disease or other condition. In some embodiments, a plurality or mixture of species may be differentially solubilized in a single two-phase aqueous system, or other multi-phase aqueous system. The nature or degree of the solubilization of the species may be used to determine the health status of a subject. For example, some embodiments are directed to devices and methods for determining a disease or other condition as a function of the changes to the structure of two or more species. The species may be selected based on their differential solubility behavior in a two-phase or other multi-phase aqueous system.

Abstract: A method of preparing an antibody therapeutic is provided comprising: (a) providing a dissociated cell sample from at least one solid tumor sample obtained from a patient; (b) loading the dissociated cell sample into a microfluidic device having a flow region and at least one isolation region fluidically connected to the flow region; (c) moving at least one B cell from the dissociated cell sample into at least one isolation region in the microfluidic device, thereby obtaining at least one isolated B cell; and (d) using the microfluidic device to identify at least one B cell that produces antibodies capable of binding to cancer cells. The cancer cells can be the patient's own cancer cells. Also provided are methods of treating patients, methods of labeling or detecting cancer, engineered T or NK cells comprising antibodies or fragments thereof, and engineered antibody constructs.

Abstract: Provided herein are methods for classifying how a subject having a cancer will respond to immunotherapeutic (IT) therapy based on the subject's immunosignature or frameshift signature. Also provided herein are methods for classifying a subject having a cancer as having a good prognosis or a poor prognosis based on the subject's immunosignature or frameshift signature.

Abstract: The present invention provides methods for screening and diagnosing prostate cancer based on a correlation between cancer cell growth, cancer lethality or recurrence and the expression level of ZFP36 or NEDD9 and PTEN in conjunction with ZFP36. The disclosure also provides methods for screening and diagnosing prostate cancer based on a correlation between cancer lethality and the genotype of rs1910301 SNP. Also provided are methods for treating prostate cancer comprising providing a treatment or monitoring based on the expression level of ZFP36, the expression level of NEDD9, and the expression level of ZFP36 in conjunction with PTEN levels or the genotype of rs1910301 SNP.

Abstract: Complexes containing a labeled polypeptide and an antibody, and the use of such complexes as research, diagnostic, and clinical tools, are described herein.

Abstract: The embodiments disclosed herein provide a peptoid, which is a compound of Formula I or a stereoisomer, a mixture of stereoisomers, or a pharmaceutically acceptable salt thereof. N—R4, N—R3, N—R2, and N—R1 in Formula I are respectively. The peptoid has a strong binding ability with an EpCAM protein. It can be used as molecular probes for diseases associated with an EpCAM protein, to achieve targeted therapy, in vivo imaging diagnosis and prognosis monitoring of the diseases associated with the EpCAM protein at low cost, high accuracy and high efficiency.

Abstract: Described herein is cancer biomarker, e.g., a vesicle, capture surface that includes a substrate, a first plurality of nanoparticles attached to the substrate; a plurality of bifunctional tethers, wherein a first functionality of the bifunctional tethers is attached to at least a portion of the first plurality of nanoparticles; a second plurality of nanoparticles attached to a second functionality of at least a portion of the plurality of bifunctional tethers; a capture agent attached to at least a portion of the second plurality of nanoparticles; and a plurality of polymer brush molecules attached to the surface, wherein the polymer brush molecules have a lower molecular weight than the bifunctional tethers, and wherein the polymer brush molecules reduce nonspecific binding to the surface. Also described are method of capturing cancer biomarkers such as vesicles from a liquid biopsy sample.

Abstract: Devices, kits, and methods related to embodiments of an improved liquid test sample injection device comprising a sample mixture that comprises at least one sample flag compound for detecting the presence or non-presence of a patient's liquid test sample upon being interrogated by a pre-determined wavelength of light.

Abstract: An analysis apparatus (1) includes a housing unit (10) that houses a biological sample (S), a staining dispenser unit (20) that fluorescently stains the biological sample (S), an image acquisition unit (30) that acquires an image of the biological sample (S), a washer unit (40) that destains the biological sample (S), a drive unit (50), and a control unit (90) that analyzes the image and controls the drive unit (50). The control unit (90) performs adjustments so that the staining dispenser unit (20) and the housing unit (10) satisfy a first position condition, performs adjustments so that the image acquisition unit (30) and the housing unit (10) satisfy a second position condition, and performs adjustments so that the washer unit (40) and the housing unit (10) satisfy a third position condition. The analysis apparatus (1) maximizes the actual photography range of information related to the biological sample.

Abstract: The present description provides methods, assays and reagents useful for sequencing proteins. Sequencing proteins in a broad sense involves observing the plausible identity and order of amino acids, which is useful for sequencing single polypeptide molecules or multiple molecules of a single polypeptide. In one aspect, the methods are useful for sequencing multiple polypeptides. The methods and reagents described herein can be useful for high resolution interrogation of the proteome and enabling ultrasensitive diagnostics critical for early detection of diseases.

Abstract: The present invention relates to methods of diagnosing glomerulonephritis (GN) in a patient, as well as methods of monitoring the progression of GN and/or methods of monitoring a treatment protocol of a therapeutic agent or a therapeutic regimen. The invention also relates to assay methods used in connection with the diagnostic methods described herein.

Abstract: Provided is an antibody composition comprising antibodies that specifically bind to 22-hydroxycholesterol, 24S-hydroxycholesterol, 25-hydroxycholesterol, 26-hydroxycholesterol or 27-hydroxycholesterol. Also provided is a method of making antibodies that specifically bind to 22-hydroxycholesterol, 24S-hydroxycholesterol, 25-hydroxycholesterol, 26-hydroxycholesterol or 27-hydroxycholesterol is provided. Further provided is a method of assaying for 22-hydroxycholesterol, 24S-hydroxycholesterol, 25-hydroxycholesterol, 26-hydroxycholesterol or 27-hydroxycholesterol is provided. Still further provided is a kit for detecting 22-hydroxycholesterol, 24S-hydroxycholesterol, 25-hydroxycholesterol, 26-hydroxycholesterol or 27-hydroxycholesterol.