Abstract: The subject matter of the invention is an adhesive tape, in particular a wrapping tape for sheathing cables (1) in automobiles, comprising a strip-type fabric backing (2, 3) that has at least one first layer (2) made of a woven fabric and a second layer (3) made of a nonwoven, the two layers being coupled together by a polymer layer (4) and/or by threads that penetrate both layers (2, 3), and comprising an adhesive layer (5) applied to at least one side of the backing (2, 3), characterised in that the nonwoven is produced from an aromatic polyamide.

Abstract: (A) an organopolysiloxane having, per molecule, two groups each represented by formula (1) (R1 represents a C1-20 monovalent hydrocarbon group, R2 represents an oxygen atom or the like, R3 represents an acryloyloxyalkyl group or the like, and p and a respectively represent numbers satisfying 0-10 and 1-3); (B) a monofunctional (meth)acrylate compound not including a siloxane structure; (C) an organopolysiloxane resin which comprises a unit (a) represented by formula (2) (R1, R2, R3, a, and p are identical to those described above), a R43SiO1/2 unit (b) (in the formula, R4 represents a monovalent hydrocarbon group having 1-10 carbon atoms), and a SiO4/2 unit (c), and in which the mole ratio of the total of units (a) and (b) to unit (c) is in a range of 0.4 to 1.2:1; and (D) a photoinitiator.

Abstract: A thermally activated delayed fluorescent material, preparing method thereof, and an organic electroluminescent device are disclosed. The organic electroluminescent device includes an anode layer, a cathode layer, and a light-emitting layer disposed between the anode layer and the cathode layer. The light-emitting layer includes a thermally activated delayed fluorescent material. A structural formula of the thermally activated delayed fluorescent material is shown in the following structural formula I: and R1-R6 are electron donor units.

Abstract: Aerogel materials, aerogel composites, and the like may be improved by the addition of opacifiers to reduce the radiative component of heat transfer. Such aerogel materials, aerogel composites, and the like may also be treated to impart or improve hydrophobicity. Such aerogel materials and methods of manufacturing the same are described.

Abstract: This invention relates to treated geothermal brine compositions containing reduced concentrations of lithium, iron and silica compared to the untreated brines. Exemplary compositions contain concentration of lithium ranges from 0 to 200 mg/kg, concentration of silica ranges from 0 to 30 mg/kg, concentration of iron ranges from 0 to 300 mg/kg. Exemplary compositions also contain reduced concentrations of elements like arsenic, barium, and lead.

Abstract: The present invention disclosed a cross-linked plugging agent stimulated by high density brine which comprises: main agent: 21 wt %-53 wt %, gelling agent: 0.5 wt %-9 wt % and pure water; wherein the saline solution is selected from the group consisting of a Dipotassium phosphate (K2HPO4), a Tripotassium phosphate (K3PO4) and a Potassium pyrophosphate (K4P2O7); the gelling agent is selected from the group consisting of a Xanthan gum and a kappa carrageenan. The gelling time is controlled within 0.5-12 h under an experiment temperature of 90° C.-160° C. The final gelling strength is controlled within D-H. The density is controlled within 1.2-1.55 g/cm3.

Abstract: A composition for lost circulation material (LCM) usable in subsurface well intervention includes particles of a hydrated superabsorbent polymer (SAP) mixed with an aqueous solution of a salt tolerant osmotic barrier polymer. A method for stopping fluid loss from a well into a formation penetrated by the well includes pumping a selected volume of the composition into the well until the selected volume is adjacent to the formation.

Abstract: Imidazoline-derived compounds are used in compositions and methods for inhibiting natural gas hydrate agglomerates. The imidazoline-derived compounds are reaction products between a tall oil fatty acid and an amine. The methods include introducing a composition downhole, the composition comprising an imidazoline-derived compound formed by reacting a tall oil fatty acid with an amine; and contacting the composition with a downhole fluid to inhibit formation of hydrate agglomerates, wherein the downhole fluid comprises natural gas or hydrocarbons.

Abstract: The present invention discloses a novel high-temperature visual acid etching test device and method; the test device includes a visual rock slab holder, an acid solution storage tank I, an acid solution storage tank II, a heating apparatus, a high-pressure water pump, a low-pressure acid pump, a back-pressure valve, an acid valve A, an acid valve B, an acid valve C, an acid valve D, an acid valve E, an acid valve F, a water valve G, a water valve H, a water valve I, a water valve J, and a water valve K; both the acid solution storage tank I and the acid solution storage tank II are provided with a piston therein, where the acid solution storage tank is divided into upper and lower independent parts by the piston. According to the present invention, the flowing process of an acid solution in a fracture can be directly observed to accurately characterize the distribution of the acid solution in the fracture, which facilitates a study on an acid etching mechanism.

Abstract: A system and method for hydraulic fracturing a subterranean formation with fracturing fluid to generate fractures, and intermittently adjusting a characteristic of the fracturing fluid conveying proppant to form pillars of proppant in the fractures.

Abstract: A pyrolysis oil fractionation system for treating a pyrolysis oil feed includes a fractionation column, at least one treatment catalyst bed, and a plurality of distillation trays. The system further includes a condenser to receive a light fraction and produce a condensed gasoline product and a vapor, a receiver coupled to the condenser, a knockout drum, and a distillate stripper coupled to the fractionation column. A method for treating a pyrolysis oil feed includes, in a fractionating column, dehydrohalogenating, decontaminating, and/or dehydrating a pyrolysis oil feed in at least one treatment catalyst bed, and distilling the treated pyrolysis oil feed into a light fraction, a middle fraction, a heavy fraction, and a bottom fraction. The method further includes condensing the light fraction and producing a condensed gasoline product and a vapor, separating a fuel gas product from the vapor, and stripping the middle fraction to produce a distillate product.

Abstract: In a method and system for treating a catalytic cracking gasoline, a catalytic cracking process, or a plant employs a fluidized reactor to carry out hydrodealkylation treatment on a catalytic cracking oil gas or catalytic cracking gasoline, so that heavy aromatics present therein can be efficiently converted into light olefins and light aromatics. The method and system can improve the yield of light olefins, allow a long-period stable operation, relieve the contradiction between supply and demand of light aromatics, and solve the problem of high content of heavy aromatics that have low value and are difficult to be utilized in aromatics present in oil gas from catalytic cracking units.

Abstract: The present invention aims to provide a viscosity index improver composition capable of providing a lubricant composition that has an excellent viscosity index improving effect, excellent antifoaming properties, and excellent persistence of the antifoaming properties. The present invention relates to a viscosity index improver composition, containing: a (co)polymer (A) containing a monomer (a) represented by the following formula (1) as an essential monomer; a C18-C40 chain aliphatic alcohol (B); and a base oil, [Chem. 1] wherein R1 is a hydrogen atom or a methyl group; —X1— is a group represented by —O— or —NH—; R2 is a C2-C4 alkylene group; R3 and R4 are each independently a C8-C24 linear or branched alkyl group; and p is an integer of 0 to 20, with each R2 being optionally the same as or different from each other when p is 2 or greater.

Abstract: The present invention provides, among other things, a process to produce a low molecular weight dispersant polymethacrylate polymer (“LMWDPMA”), and the LMWDPMA itself, along with the use of the LMWDPMA in a lubricating composition, as well as a method for lubricating an internal combustion engine as disclosed herein.

Abstract: A zinc coordination organoselenium polymer may be a catalyst. The zinc coordination polymer may be a catalyst for biodiesel fuel. A method of forming biodiesel from waster oil may include using the zinc coordination polymer. The waste oil may be selected from a group consisting of vegetable oil and animal fats.

Abstract: A detergent composition is a detergent composition for removing temporary adhesive containing a silicone compound that is present on a substrate. The detergent composition contains: (A) an organic solvent: 75 to 99 parts by mass; (B) water: 0 to 5 parts by mass; and (C) an ammonium salt: 1 to 20 parts by mass (where (A)+(B)+(C)=100 parts by mass). The organic solvent does not contain an organic solvent having a hydroxy group and contains, in 100 parts by mass of the organic solvent, 50 parts by mass or more of an organic solvent having a heteroatom. The ammonium salt contains at least one kind of a hydroxide ion, a fluoride ion, and a chloride ion.

Abstract: A composition including a plurality of particles, each of the particles including: about 40% to about 90% by weight of the particles water soluble polymer; about 1% to about 40% by weight of the particles of a salt hydrate; and about 0.1% to about 20% by weight of the particles perfume; wherein the perfume is dispersed in a matrix of the water soluble polymer and the salt hydrate. A process for forming particles that includes using an anhydrous salt to manage water.

Abstract: A sample is introduced from a bioreactor to a chamber included in a manifold. The chamber includes at least a first probe. The at least the first probe is coupled to a controller. One or more measurements are performed on the sample while the sample is located in the chamber for a measurement period. The sample is returned from the chamber to the bioreactor after the one or more measurements of the sample have been performed.

Abstract: The present invention relates to a process for producing enriched algal biomass having high lipid productivity. More particularly, the present invention provides a process for obtaining an enriched biomass with omega-3 fatty acids by using a microalgal strain Schizochytrium MTCC 5890 in a unique media composition and substrate residual band in a steady state continuous fermentation. The process of the present invention results in high biomass and lipid productivity.

Abstract: Provided herein are compositions and methods for reducing bacterial contamination during cell culture. Such compositions and methods utilize engineered peptides or recombinant cells capable of secreting such peptides into culture medium. Also provided are methods of using the engineered peptides for inhibiting bacterial growth during culturing of cells.

Abstract: A discontinuous wall magnet having an opening or channel is provided. A bead separation magnet having a discontinuous or segmented wall is also provided. The segmented wall causes bead formation to form in a segmented or gapped ring to allow for easier manual pipetting. Also provided are systems and kits having the inventive magnets. Methods of purifying a macromolecule using the inventive magnets are also provided.

Abstract: This invention pertains to mutant Cas9 nucleic acids and proteins for use in CRISPR/Cas endonuclease systems, and their methods of use. In particular, the invention pertains to an isolated mutant Cas9 protein, wherein the isolated mutant Cas9 protein is active in a CRISPR/Cas endonuclease system, wherein the CRISPR/Cas endonuclease system displays reduced off-target editing activity and maintained on-target editing activity relative to a wild-type CRISPR/Cas endonuclease system. The invention also includes isolated nucleic acids encoding mutant Cas9 proteins, ribonucleoprotein complexes and CRISPR/Cas endonuclease systems having mutant Cas9 proteins that display reduced off-target editing activity and maintained on-target editing activity relative to a wild-type CRISPR/Cas endonuclease system.

Abstract: The present embodiments provide methods, compounds, and compositions useful for inhibiting APOL1 expression, which may be useful for treating, preventing, or ameliorating a disease associated with APOL1.

Abstract: One aspect of the present invention relates to double-stranded RNA (dsRNA) agent capable of inhibiting the expression of a target gene. The sense strand of the dsRNA agent comprises at least one thermally destabilizing nucleotide, and at least one said thermally destabilizing nucleotide occurring at a site opposite to the seed region (positions 2-8) of the antisense strand; and the antisense strand of the dsRNA agent comprises at least two modified nucleotides that provide the nucleotide a steric bulk that is less than or equal to the steric bulk of a 2?-OMe modification, wherein said modified nucleotides are separated by 11 nucleotides in length. Other aspects of the invention relates to pharmaceutical compositions comprising these dsRNA agents suitable for therapeutic use, and methods of inhibiting the expression of a target gene by administering these dsRNA agents, e.g., for the treatment of various disease conditions.

Abstract: The present invention is directed to a yeast strain, or strains, secreting a full suite, or any subset of that full suite, of enzymes to hydrolyze corn starch, corn fiber, lignocellulose, (including enzymes that hydrolyze linkages in cellulose, hemicellulose, and between lignin and carbohydrates) and to utilize pentose sugars (xylose and arabinose). The invention is also directed to the set of proteins that are well expressed in yeast for each category of enzymatic activity. The resulting strain, or strains can be used to hydrolyze starch and cellulose simultaneously. The resulting strain, or strains can be also metabolically engineered to produce less glycerol and uptake acetate. The resulting strain, or strains can also be used to produce ethanol from granular starch without liquefaction.

Abstract: The present invention provides high efficiency targeted and marker-less single, double, triple, quadruple, and quintuple integrations by using CRISPR in host cells, including Pichia.

Abstract: The invention provides DNA molecules and constructs, including their nucleotide sequences, useful for modulating gene expression in plants and plant cells. Transgenic plants, plant cells, plant parts, seeds, and commodity products comprising the DNA molecules operably linked to heterologous transcribable polynucleotides are also provided, as are methods of their use.

Abstract: The invention provides recombinant DNA molecules and constructs, as well as their nucleotide sequences, useful for modulating gene expression in plants. The invention also provides transgenic plants, plant cells, plant parts, and seeds comprising the recombinant DNA molecules operably linked to heterologous transcribable DNA molecules. Also provided are methods of the use of the recombinant DNA molecules and constructs and the transgenic plants, plant cells, plant parts, and seeds comprising the recombinant DNA molecules and constructs.

Abstract: The invention provides methods of transforming photosynthetic organisms, such as green algae. The methods involve methylating one or more DNA fragments of a DNA construct and transforming the one or more fragments into the photosynthetic organism. The DNA fragments can be the product of a DNA amplification procedure, such as PCR or a PCR-like procedure. In one embodiment the one or more fragments of DNA that comprise a DNA construct are dam methylated prior to being transformed into the photosynthetic organism.

Abstract: The present disclosure provides plants that express a variant phosphoenolpyruvate carboxylase (PEPC) enzyme. The plants have enhanced resistance to aluminum than comparable plants that lack the variant PEPC enzyme. In addition, the plants more effectively sequester carbon, extract phosphate, and produce oxaloacetate-derived amino acids and glucose than comparable plants that lack the variant PEPC enzyme. The disclosure also provides tools for production of plants that express the variant PEPC enzyme.

Abstract: Compositions and methods for controlling pests are provided. The methods involve transforming organisms with a nucleic acid sequence encoding an insecticidal protein. In particular, the nucleic acid sequences are useful for preparing plants and microorganisms that possess insecticidal activity. Thus, transformed bacteria, plants, plant cells, plant tissues and seeds are provided. Compositions are insecticidal nucleic acids and proteins of bacterial species. The sequences find use in the construction of expression vectors for subsequent transformation into organisms of interest including plants, as probes for the isolation of other homologous (or partially homologous) genes. The pesticidal proteins find use in controlling, inhibiting growth or killing Hemipteran, Lepidopteran, Coleopteran, Dipteran, fungal, and nematode pest populations and for producing compositions with insecticidal activity.

Abstract: Compositions and methods for modulating male fertility in a plant are provided. Compositions comprise nucleotide sequences, and active fragments and variants thereof, which modulate male fertility. Further provided are expression cassettes comprising the male fertility polynucleotides, or active fragments or variants thereof, operably linked to a promoter, wherein expression of the polynucleotides modulates the male fertility of a plant. Various methods are provided wherein the level and/or activity of the sequences that influence male fertility is modulated in a plant or plant part.

Abstract: The present disclosure provides nucleic acid molecules comprising a first inverted terminal repeat (ITR), a second ITR, and a genetic cassette encoding a miRNA and/or a therapeutic protein. In certain embodiments, the therapeutic protein comprises a clotting factor, e.g., a FVIII polypeptide, a FIX polypeptide, or a fragment thereof. In some embodiments, the first ITR and/or the second ITR is an ITR of a non-adeno-associated virus (AAV). The present disclosure also provides methods of treating bleeding disorders such as hemophilia comprising administering to the subject the nucleic acid molecule or a polypeptide encoded thereby.

Abstract: Methods for preparing and purifying viral particles, and compositions and uses comprising the same, are provided.

Abstract: The present disclosure provides methods for generating induced cardiomyocytes and/or inducing a cardiomyocyte phenotype in cells in vivo or in vitro, such as by expression of ASCL1 or MYF6 and MYOCD. The present disclosure further provides gene-delivery vectors comprising one or more polynucleotides selected from ASCL1, MYF6, MYOCD, MEF2C, and TBX5. It further provides compositions comprising induced cardiomyocytes and provides methods of treating a heart condition, such as myocardial infarction. The disclosure also provides engineered myocardin proteins with an internal deletion, vectors encoding such engineered mycocardins, and methods of use thereof.

Abstract: A method and related apparatus for confirming whether a kill laser successfully destroys an undesired population of cells includes introducing fluorescent dye into cells, exciting the cells with a detection laser or a light emitting diode to cause the cell to fluoresce for a first time, measuring the amount of fluorescence in the cells with a detector capable of emitting a detection pulse, classifying the cells via embedded processing as undesired or desired cells based on the amount of fluorescence, firing a kill beam with a kill laser at any undesired cells, measuring the amount of fluorescence in the cells a second time to determine whether a fluorescent event was generated from the kill beam striking the cells, and providing feedback to an operator of the kill laser as to whether any fluorescent events were generated from the kill beam striking the cells.

Abstract: The invention is directed to modified T cells, methods of making and using isolated, modified T cells, and methods of using these isolated, modified T cells to address diseases and disorders. In one embodiment, this invention broadly relates to TCR-deficient T cells, isolated populations thereof, and compositions comprising the same. In another embodiment of the invention, these TCR-deficient T cells are designed to express a functional non-TCR receptor. The invention also pertains to methods of making said TCR-deficient T cells, and methods of reducing or ameliorating, or preventing or treating, diseases and disorders using said TCR-deficient T cells, populations thereof, or compositions comprising the same.

Abstract: The present invention relates to a method for producing T cells or NK cells including culturing T cells or NK cells in a culture medium containing a CaMKII inhibitor such as a bisbenzylisoquinoline alkaloid compound. The disclosed compounds and methods can be used for maintaining the undifferentiated state of undifferentiated T cells, or for efficiently proliferating T cells or NK cells.

Abstract: A method for culturing heart tissue by culturing a slice of the heart tissue in a culture medium, wherein the culture medium comprises fetal bovine serum (FBS), vascular endothelial cell growth factor (VEGF), and fibroblast growth factor (FGF); and applying electrical stimulation to the slice while the heart slice is in culture is provided. Methods of screening candidate therapeutic agents for therapeutic effect or cardiotoxicity using the culture system are also provided.

Abstract: A system and method for preparing a cancer vaccine (and optionally purifying the blood) has a blood filtration system, controlled by a processing unit, for filtering exogenous blood plasma to isolate tumor cells, tumor stem cells and tumor breakdown products. The blood filtration system filter may include multiple layers having differently sized apertures to retain differently sized materials (from among tumor cells of different sizes, tumor stem cells and tumor breakdown products. A device directs electromagnetic radiation at the separated tumor cells, tumor stem cells and/or tumor breakdown products. The electromagnetic radiation may cause the separated tumor cells, separated tumor stem cells and/or separated tumor breakdown products (for example tumor protein such as DNA and/or tumor exosomes) to have a coagulated outer layer such as by degrading the outer surface. The electromagnetic radiation may have a UV wavelength. A conical coil improves flow rate uniformity. Tumor exosomes may be centrifuged.

Abstract: The subject invention concerns materials and methods for treating or preventing disease and conditions associated with various sulfatase enzymes that are defective or that are not properly expressed in a person or animal. In one embodiment, the disease is Sanfilippo A (MPS-IIIA) disease. The subject invention also concerns materials and methods for treating or preventing multiple sulfatase deficiency (MSD) in a person or animal. Compounds of the invention include a fusion protein comprising i) a mammalian sulfatase, or an enzymatically active fragment or variant thereof, and ii) a plant lectin or a binding subunit thereof. In a specific embodiment, the mammalian sulfatase is a human sulfatase, or an enzymatically active fragment or variant thereof. Polynucleotides encoding the fusion proteins are also contemplated for the subject invention. The subject invention also concerns materials and methods for producing proteins of the invention.

Abstract: Provided is a recombinant KOD polymerase, which is the following A) or B): the polymerase shown in A) is a protein having DNA polymerase activity that is obtained by modifying amino acid residues in at least one of the following 18 positions in a wild-type KOD DNA polymerase amino acid sequence: 675th, 385th, 710th, 674th, 735th, 736th, 606th, 709th, 347th, 349th, 590th, 676th, 389th, 589th, 680th, 384th, 496th and 383rd; the polymerase described by B) is a protein having DNA polymerase activity that is derived from A) by adding a tag sequence to an end of the amino acid sequence of the protein shown in A).

Abstract: Provided are methods and compositions for activating oligonucleotide aptamer-deactivated DNA polymerases, comprising modifying the aptamer by uracil-DNA glycosylase enzymatic activity to reduce or eliminate binding of the oligonucleotide aptamer to the DNA polymerase, thereby activating DNA synthesis activity of the DNA polymerase in a reaction mixture. Mixtures for use in methods of the invention are also provided. In some aspects, the oligonucleotide aptamers are circular and comprise one or more deoxyuridine nucleotides providing for aptamer-specific recognition and modification of the circular aptamer by the uracil-DNA glycosylase enzymatic activity. Exemplary oligonucleotide aptamers, mixtures and methods employing uracil-DNA glycosylase enzymatic activity are provided.

Abstract: A process of recovering oil, comprising (a) converting a starch-containing material into dextrins with an alpha-amylase; (b) saccharifying the dextrins using a carbohydrate source generating enzyme to form a sugar; (c) fermenting the sugar in a fermentation medium into a fermentation product using a fermenting organism; (d) recovering the fermentation product to form a whole stillage; (e) separating the whole stillage into thin stillage and wet cake; (e?) optionally concentrating the thin stillage into syrup; (f) recovering oil from the thin stillage and/or optionally the syrup, wherein a protease and a phospholipase are present and/or added during steps (a) to (c). Use of a protease and a phospholipase for increasing oil recovery yields from thin stillage and/or syrup in a fermentation product production process.

Abstract: The present disclosure relates to compositions, kits, and methods of making RNA vaccines having an appropriate cap structure. Systems, apparatus, compositions, and/or methods may include and/or use, in some embodiments, non-naturally occurring single-chain RNA capping enzymes. In some embodiments, an RNA capping enzyme may include an FCE variant having (a) an amino acid sequence at least 90% identical to positions 1 to 878 of SEQ ID NO: 1, and/or (b) one or more substitutions relative to SEQ ID NO: 1 at a position selected from positions corresponding to positions 215, 337, 572, 648, and 833 (e.g., a position selected from positions corresponding to position 215, 337, and 572) of SEQ ID NO: 1.

Abstract: The invention provides for systems, methods, and compositions for altering expression of target gene sequences and related gene products. Provided are structural information on the Cas protein of the CRISPR-Cas system, use of this information in generating modified components of the CRISPR complex, vectors and vector systems which encode one or more components or modified components of a CRISPR complex, as well as methods for the design and use of such vectors and components. Also provided are methods of directing CRISPR complex formation in eukaryotic cells and methods for utilizing the CRISPR-Cas system. In particular the present invention comprehends optimized functional CRISPR-Cas enzyme systems. In particular the present invention comprehends engineered new guide architectures and enzymes to be used in optimized Staphylococcus aureus CRISPR-Cas enzyme systems.

Abstract: Disclosed are a medium composition for culturing Clostridium botulinum comprising a potato peptone, a yeast extract and glucose, and a method of preparing a botulinum toxin using the same. The medium excludes animal-derived products and major allergens, and enables the botulinum toxin-producing strain to reach the maximum growth level within a short time, thereby providing effects of shortening the production time and reducing the production cost in the botulinum toxin production process.

Abstract: The present invention relates to a method for modifying the glycosylation profile of a recombinant glycoprotein produced in cell culture comprising culturing eukaryotic cells expressing the recombinant glycoprotein in a cell culture medium, wherein the cell culture medium is supplemented with fucose, manganese, and taurine, wherein the glycosylation profile of the produced recombinant glycoprotein is modified to better resemble the glycosylation profile of a reference glycoprotein than when cultured without said supplementation.

Abstract: Processes are described herein for realizing an enhanced yield of ethanol and co-products from processes for producing ethanol from biomass containing lignocellulosic material. Also described herein are product outputs of these processes having new and useful properties and systems implementing the processes.

Abstract: A method for enzymatically preparing a n-3 polyunsaturated fatty acid diacylglycerol using oil rich in n-3 polyunsaturated fatty acid as raw material to prepare diacylglycerol oil with selective enzymatic hydrolysis. Before the enzymatic reaction, the raw materials are homogenized to improve the catalytic efficiency of lipase in the hydrolysis and achieve the purpose of reducing the reaction time and the addition amount of lipase. As a result, the cost of industrial diacylglycerol preparation is reduced, ultimately reducing the cost of industrial diacylglycerol preparation. Therefore, the present invention provides an effective, mild and low-cost enzymatic method for preparing diacylglycerol rich in n-3 polyunsaturated fatty acids, which has a promising potential for industrial application.