Abstract: A flow cell for confining molecules in a fluid. The flow cell includes an upper substrate, an upper support member, a center substrate, a membrane, a lower support member and a lower substrate. The lower support member comprises an imaging chamber it is positioned below the membrane and above the lower substrate. In one embodiment the membrane comprises a nanopore and nanoscale groove extending through the membrane. In another embodiment the lower substrate comprises an upper face in communication with the imaging chamber, and the upper face comprises a plurality of nanoscale grooves extending partially through the lower substrate. In both embodiments the upper substrate, upper support member, center substrate and membrane are displaceable through the imaging chamber, thereby causing molecules in the imaging chamber to be confined or trapped through the nanoscale groove(s) of the membrane or of the lower substrate.

Abstract: Systems and methods for analysis of liquids by covered fluidic channels integrated onto sensor platforms. According to an aspect, a method includes receiving at least one of a liquid and an analyte of interest into a covered fluidic channel with a predetermined orientation. The method also includes confining at least one of the liquid and the analyte of interest within the covered fluidic channel. The method further includes analyzing properties of at least one of the liquid and the analyte of interest.

Abstract: Systems, devices and methods are provided for fabricating anisotropic polymer materials. According to various embodiments, a fluidic device is employed to distribute a polymer solution and a flow-confining solution in order to generate a layered flow, where the layered flow is formed such that a polymer liquid sheet is sheathed on opposing sides by flow-confining liquid sheets. The fluidic device includes first and second fluid conduits, where the first fluid conduit receives the layered flow. The second fluid conduit has a reduced height relative to the first fluid conduit, such that the layered flow is constricted as it flows through the second fluid conduit. The constriction formed by the second flow conduit causes hydrodynamic focusing, reducing the thickness of the polymer liquid sheet, and inducing molecular alignment and anisotropy within the polymer liquid sheet as it is hardened and as strain is applied during extrusion of the sheet.

Abstract: A micro-fluidic system for a perfusion cell culture is disclosed. The system includes: a substrate; a micro-fluid injection channel defined in the substrate to guide fluid in a plane direction of the substrate; at least two micro-fluid branch channels defined in the substrate, wherein the branch channels are branched from the micro-fluid injection channel; micro-fluid outlet channels defined in the substrate, wherein each of the outlet channels extends from a distal end of each branch channel to a top face of the substrate, wherein each outlet channel has each through-hole defined in the top face portion of the substrate; and well plates disposed on the top face of the substrate, wherein each of the well plates fluid-communicates with each outlet channel. The micro-fluidic system refers to a technique that adjusts a flow of liquid or gas of a very small amount (nanoliter or picoliter) in an extremely miniaturized device.

Abstract: A microfluidic device for storing a reagent includes a single unit includes a first portion having a reagent storage chamber configured to hold a reagent. The device also includes a second portion having a reaction chamber configured to support the reagent during a reaction process to form a product. The device also includes a valve configured to isolate the reagent storage compartment from the reaction chamber when the valve is in a closed state.

Abstract: Multilevel microfluidic devices include a control line that can simultaneously actuate valves for both sample and reagent lines. Microfluidic devices are configured to contain a first reagent in a first chamber and a second reagent in a second chamber, where either or both of the first and second reagents are contained at a desired or selected pressure. Operation of a microfluidic device includes transmitting second reagent from the second chamber to the first chamber, for mixing or contact with the first reagent. Microfluidic device features such as channels, valves, chambers, can be at least partially contained, embedded, or formed by or within one or more layers or levels of an elastomeric block.

Abstract: A microfluidic device and a method of fabricating the microfluidic device are provided. The microfluidic device includes: a platform including an upper substrate and a lower substrate that are bonded to face each other; a microfluidic structure obtained by forming grooves in the lower substrate; a lower substrate protrusion pattern including an outline protrusion that protrudes from the lower substrate toward the upper substrate along an outline of the microfluidic structure; and an adhesive layer disposed between the lower substrate protrusion pattern and the upper substrate in order to bond the upper substrate and the lower substrate to each other. The lower substrate protrusion pattern only supports the upper substrate, and remaining portions of the lower substrate except for the lower substrate protrusion pattern do not have structures for supporting the upper substrate.

Abstract: A by-pass fluid flow amplifier which contains a primary nozzle and a primary profile for discharging compressed air into a conduit of the air amplifier and entraining ambient air in the process. A secondary nozzle and a secondary profile for discharging compressed air into the conduit of the air amplifier towards the rear that assists the primary nozzle such as to allow consistent fluid wall attachment of the compressed air and the entrained air caused by the primary nozzle and the primary profile. The secondary nozzle increases the total flow of the amplifier and prohibits the total flow from traveling towards the center of the conduit where flow reversal and turbulence are likely to occur.

Abstract: A method of fabricating an elastomeric structure, comprising: forming a first elastomeric layer on top of a first micromachined mold, the first micromachined mold having a first raised protrusion which forms a first recess extending along a bottom surface of the first elastomeric layer; forming a second elastomeric layer on top of a second micromachined mold, the second micromachined mold having a second raised protrusion which forms a second recess extending along a bottom surface of the second elastomeric layer; bonding the bottom surface of the second elastomeric layer onto a top surface of the first elastomeric layer such that a control channel forms in the second recess between the first and second elastomeric layers; and positioning the first elastomeric layer on top of a planar substrate such that a flow channel forms in the first recess between the first elastomeric layer and the planar substrate.

Abstract: A device for processing fluids includes a filter to capture a cell sample. The filter has a physical barrier to isolate the cell sample on the filter. Growth detection circuitry is associated with the filter. The growth detection circuitry identifies, through electrical measurements, a cell growth rate and hence the presence of live cells associated with the cell sample.

Abstract: The present invention relates to fluidic systems, including switches for fluidic systems. The switches of the present invention may be particularly applicable to microfluidic systems. The switches of the invention may include a switching region having more than one position corresponding to more than one aspect ratio. Alternatively, the switches of the invention may include multiple inlets and a system for the selective supply of carrier fluid. The present invention also relates to a method of controlling a fluid in a microfluidic system and may be performed using the switches of the present invention.

Abstract: Microfluidic devices with multiple fluid process regions for subjecting similar samples to different process conditions in parallel are provided. One or more common fluid inputs may be provided to minimize the number of external fluid supply components. Solid materials such as chromatographic separation media or catalyst media is preferably provided in each fluid process region. Solid materials may be supplied to the devices in the form of slurry, with particles retained by porous elements or frits. Different fluid process regions may having different effective lengths, different solid material types or amounts, or may receive different ratios of common fluids supplied to the device. The flow resistances of dissimilar fluid process regions may be balanced passively with the addition of impedance elements in series with each fluid process region.

Abstract: A microfluidic reactor for performing chemical and biological synthesis reactions, including chemical and biological syntheses of organic, polymer, inorganic, oligonucleotide, peptide, protein, bacteria, and enzymatic products is provided. Two fluids are input into the device, mixed in a mixing region and provided to a long, composite reaction channel. Fluids flowing through the reaction channel may be diverted at a diversion region into a sample channel. Fluids in the sample channel may be mixed at a second region, with additional reagents.

Abstract: The present invention relates to fluidic systems, including switches for fluidic systems. The switches of the present invention may be particularly applicable to microfluidic systems. The switches of the invention may include a switching region having more than one position corresponding to more than one aspect ratio. Alternatively, the switches of the invention may include multiple inlets and a system for the selective supply of carrier fluid. The present invention also relates to a method of controlling a fluid in a microfluidic system and may be performed using the switches of the present invention.

Abstract: A microfluidic reactor for performing chemical and biological synthesis reactions, including chemical and biological syntheses of organic, polymer, inorganic, oligonucleotide, peptide, protein, bacteria, and enzymatic products is provided. Two fluids are input into the device, mixed in a mixing region and provided to a long, composite reaction channel. Fluids flowing through the reaction channel may be diverted at a diversion region into a sample channel. Fluids in the sample channel may be mixed at a second region, with additional reagents.

Abstract: An analog electro-fluidic signal transducer utilizing one or a tandem series of laminar proportional amplifiers. The transducer has input passages at a varying pressure differential produced by movements of a piezoelectric bender bimorph between opposed nozzles in a pressurized chamber. The pressure in the chamber and the excursion of the bender between the nozzles relative to the nozzle diameters are limited to minimize turbulence and achieve a pressure differential between the input passages that varies proportionally to or as an analog of a voltage applied to the bender. The pressure differential between the input passages is amplified by the amplifier or plural amplifiers in tandem series.

Abstract: A fluidic amplifier or switch for supplying pulses of a power gas to be controlled which is insensitive to load impedance or back pressure. One arm of the fluidic switch comprising part of the gas injection path is coupled to a working outlet and the other arm is coupled through a sonic orifice to a vent outlet. An orifice is provided in the downstream end of the power gas injection path, the injection pressure is maintained at preferably at least about twice the maximum back pressure and a substitute gas is injected into the arm of the fluidic switch not carrying the power gas to be controlled. The two gases are each alternately supplied to the working outlet and the vent outlet. When the power gas to be controlled is supplied to the working outlet, the substitute gas is supplied to the vent outlet and when the substitute gas is supplied to the working outlet, the power gas to be controlled is supplied to the vent outlet to maintain the pressure constant across the working outlet orifice.

Abstract: A fluidic oscillator includes a chamber having a common inflow and outflow opening into which a jet is issued in a generally radial direction. After impinging upon the far chamber wall the jet is redirected to form a vortex on each side of the incoming jet. The vortices alternate in strength and position to direct outflow through the common opening along one side and then the other of the inflowing jet. A spray-forming output chamber is arranged to receive the pulsating outflows from the aforementioned or other fluid oscillator and establish an output vortex which is thereby alternately spun in opposite directions. An outlet opening from the output chamber issues fluid in a sweeping spray pattern determined by the vectorial sum of a first vector, tangential to the output vortex and a function of the spin velocity, and a second vector, directed radially from the vortex and determined by the static pressure in the chamber.

Abstract: A fluidic flow control device comprising at least two generally aligned members having tapering bores therethrough, the members being so arranged as to constitute a convergence followed by a gradual divergence, separated by a gap, the gap communicating with an inlet/outlet or feed port. A cage connects the adjacent ends of the members and forms said port. A system for pumping may incorporate the device between a cylinder charged with pressure gas and an out-feed pipe. In operation, fluid is alternatively drawn in through the gap and into the cylinder and then pumped out by the gas across the gap and into and through the out-feed pipe. A level detector in the cylinder controls the oscillation of the system and an accumulator may be provided to smooth the outflow. Substantially continuous flow may be provided by coupling two systems to a common out-feed pipe.

Abstract: A fluidic oscillator includes a chamber having a common inflow and outflow opening into which a jet is issued in a generally radial direction. After impinging upon the far chamber wall the jet is redirected to form a vortex on each side of the incoming jet. The vortices alternate in strength and position to direct outflow through the common opening along one side and then the other of the inflowing jet. A spray-forming output chamber is arranged to receive the pulsating outflows from the aforementioned or other fluid oscillator and establish an output vortex which is thereby alternately spun in opposite directions. An outlet opening from the output chamber issues fluid in a sweeping spray pattern determined by the vectorial sum of a first vector, tangential to the output vortex and a function of the spin velocity, and a second vector, directed radially from the vortex and determined by the static pressure in the chamber.

Abstract: A fluidic oscillator includes a chamber having a common inflow and outflow opening into which a jet is issued in a generally radial direction. After impinging upon the far chamber wall the jet is redirected to form a vortex on each side of the incoming jet. The vortices alternate in strength and position to direct outflow through the common opening along one side and then the other of the inflowing jet. A spray-forming output chamber is arranged to receive the pulsating outflows from the aforementioned or other fluid oscillator and establish an output vortex which is thereby alternately spun in opposite directions. An outlet opening from the output chamber issues fluid in a sweeping spray pattern determined by the vectorial sum of a first vector, tangential to the output vortex and a function of the spin velocity, and a second vector, directed radially from the vortex and determined by the static pressure in the chamber.