Dielectrophoresis (i.e., Using Nonuniform Electric Field) Patents (Class 204/547)
  • Patent number: 10821439
    Abstract: A microfluidic apparatus is provided having one or more sequestration pens configured to isolate one or more target micro-objects by changing the orientation of the microfluidic apparatus with respect to a globally active force, such as gravity. Methods of selectively directing the movements of micro-objects in such a microfluidic apparatus using gravitational forces are also provided. The micro-objects can be biological micro-objects, such as cells, or inanimate micro-objects, such as beads.
    Type: Grant
    Filed: July 26, 2017
    Date of Patent: November 3, 2020
    Assignee: Berkeley Lights, Inc.
    Inventors: Keith J. Breinlinger, Eric D. Hobbs, Daniele Malleo, J. Tanner Nevill, Mark P. White
  • Patent number: 10816536
    Abstract: A bioparticle observation apparatus includes a dielectrophoresis electrode that outputs a first signal causing a dielectrophoresis force to act on a bioparticle, a sensor electrode that detects an impedance difference between the bioparticle and the liquid, and a control circuit that controls the first signal so that the detected impedance difference is fixed.
    Type: Grant
    Filed: June 10, 2019
    Date of Patent: October 27, 2020
    Assignees: SHARP KABUSHIKI KAISHA, KYOTO UNIVERSITY
    Inventors: Takeshi Mitsunaka, Yuichi Ogawa
  • Patent number: 10813688
    Abstract: A method for treating Chronic Obstructive Pulmonary Disease (COPD) or chronic bronchitis to alleviate the discomforts of breathing by using non-thermal electroporation energy to ablate diseased portions of the lung including the bronchus, airways and alveoli which, in effect, opens the restrictive diseased portions thereby maximizing the overall surface area thereof causing improved airflow and uninhibited breathing.
    Type: Grant
    Filed: November 23, 2015
    Date of Patent: October 27, 2020
    Assignee: AngioDynamics, Inc.
    Inventors: Robert Pearson, Mark Ortiz, Peter Callas
  • Patent number: 10780444
    Abstract: A system and method for detection of cells is disclosed. Target cells, such as circulating tumor cells (CTCs), may be of interest. Magnetic beads may be bound to the target cells. After which, the target cells (with the magnetic beads attached thereto) may be identified using an applied magnetic field. In one example, magnetic sensors may be used to detect movement of the target cells responsive to an applied magnetic field. In another example, an optical sensor (such as a camera) may be used to detect movement of the target cells responsive to an applied magnetic field. Further, separate from identification of the target cells, the target cells may be sorted using an applied magnetic field. In this way, a magnetic field may be used in either or both of target cell identification or target cell sorting in order to detect target cells of interest.
    Type: Grant
    Filed: January 4, 2018
    Date of Patent: September 22, 2020
    Assignee: Microsensor Labs, LLC
    Inventor: Peng Liu
  • Patent number: 10722887
    Abstract: Method, apparatus, and computer program product for a microfluidic channel having a cover opposite its bottom, the cover allowing visual inspection inside the channel, and having electrodes with patterned planar conducting materials, integrated onto its bottom. Using the planar conducting materials, once a fluid sample with suspended microparticles is applied into the channel, highly localized modulated electric field distributions are generated inside the channel and the fluid sample. This generated field causes inducing of dielectrophoretic (DEP) forces such that the DEP forces gradually increase along the length of the channel occupied by the electrodes. These DEP forces counteract the hydrodynamic drag of the flow acting on the particles suspended in the fluid. Because of the induced forces, micro/nano-particles in the fluid sample are deflected at locations in the microchannel that are a function of the particles velocity and this effect is captured by an image sensing device.
    Type: Grant
    Filed: March 21, 2017
    Date of Patent: July 28, 2020
    Assignee: International Business Machines Corporation
    Inventors: Jaione Tirapu Azpiroz, Emmanuel Delamarche, Yuksel Temiz
  • Patent number: 10705011
    Abstract: A method for dynamic focusing is presented that can be performed by a dynamic focusing controller that can receive images from the image capture device, and for each image, determine a border of the particle within the image, and calculate a pixel intensity ratio of the image based on the border of the particle. The dynamic focusing controller can also calculate a median pixel intensity ratio from the pixel intensity ratios for each image, determine a focal distance direction based on the median pixel intensity ratio, calculate a focal distance based on the median pixel intensity ratio when the focal distance direction is positive, and calculate the focal distance based on a median border width when the focal distance direction is negative. The autofocusing controller can then send an instruction to the focusing mechanism to adjust the image capture device by the focal distance in the focal distance direction.
    Type: Grant
    Filed: October 5, 2017
    Date of Patent: July 7, 2020
    Assignee: Beckman Coulter, Inc.
    Inventors: Jiuliu Lu, Bian Qian, Bart J. Wanders, Ken Good, John Riley
  • Patent number: 10684254
    Abstract: The present invention provides a method for detecting an analyte with high sensitivity. In the present method, a solution is supplied onto a substrate comprising a first electrode and a second electrode. Then, an alternating voltage is applied between the first electrode and the second electrode to aggregate, onto the surface between the first electrode and the second electrode by dielectrophoresis, bioparticles and dielectric particles contained in the solution. The aggregated bioparticles are broken to release the analyte contained in the bioparticles. The released analyte is bound to a first antibody and a second antibody to cause the dielectric particles to be immobilized onto the substrate through formation of a sandwich structure composed of the first antibody, the analyte, and the second antibody. Finally, the analyte is detected through the fluorescent substance contained in the immobilized dielectric particles.
    Type: Grant
    Filed: December 1, 2016
    Date of Patent: June 16, 2020
    Assignee: Panasonic Intellectual Property Management Co., Ltd.
    Inventors: Yasuaki Okumura, Tatsurou Kawamura, Noriko Shimba
  • Patent number: 10627719
    Abstract: Methods are described for removing edge bead on a wafer associated with a resist coating comprising a metal containing resist compositions. The methods can comprise applying a first bead edge rinse solution along a wafer edge following spin coating of the wafer with the metal based resist composition, wherein the edge bead solution comprises an organic solvent and an additive comprising a carboxylic acid, an inorganic fluorinated acid, a tetraalkylammonium compound, or a mixture thereof. Alternatively or additionally, the methods can comprise applying a protective composition to the wafer prior to performing an edge bead rinse. The protective composition can be a sacrificial material or an anti-adhesion material and can be applied only to the wafer edge or across the entire wafer in the case of the protective composition. Corresponding apparatuses for processing the wafers using these methods are presented.
    Type: Grant
    Filed: August 11, 2017
    Date of Patent: April 21, 2020
    Assignee: Inpria Corporation
    Inventors: Mollie Waller, Brian J. Cardineau, Kai Jiang, Alan J. Telecky, Stephen T. Meyers, Benjamin L. Clark
  • Patent number: 10611135
    Abstract: Embodiments of the present invention relate to a device comprising a platform comprising a layer of a 2-dimensional material. The device further comprises a plurality of electrodes and one or more molecules arranged on the platform. The device is configured to apply control signals to the plurality of electrodes to position the molecules by means of an electric field. Embodiments of the invention further concern a corresponding method for fabricating such a device and a method for positioning molecules by such a device.
    Type: Grant
    Filed: March 15, 2017
    Date of Patent: April 7, 2020
    Assignee: INTERNATIONAL BUSINESS MACHINES CORPORATION
    Inventor: Pio Peter N. Nirmalraj
  • Patent number: 10612155
    Abstract: Provided are a protein crystal device and method for crystallizing protein capable of generating protein crystal without imparting a heat effect, a protein crystal-cutting device and method for cutting protein crystal capable of cutting protein crystal without imparting a heat effect on protein crystal, and bubble-jetting member and protein-adsorbing-bubble-jetting member used in said device. A bubble-jetting member is used in a protein crystal device to jet bubbles into a protein solution to thereby allow protein crystals to be obtained, the bubble-jetting member comprising: a core formed of a conductive material; a shell part formed of an insulating material, including an extended section extending from the tip of the core, and in which at least a portion closely adheres to the core to cover the core; and a gap having a bubble-jetting port, the gap being formed between the extended section and the tip of the core.
    Type: Grant
    Filed: August 27, 2014
    Date of Patent: April 7, 2020
    Assignee: JAPAN SCIENCE AND TECHNOLOGY AGENCY
    Inventors: Yoko Yamanishi, Fumihito Arai, Hiroki Kuriki
  • Patent number: 10571475
    Abstract: Methods and systems for rapid and efficient screening of monoclonal antibodies and antibody-secreting cells, and particularly of single antibody-secreting cells, for both primary and functional characteristics, and particularly cell to cell interactions, in a microfluidic system, in particular an inverted open microwell system, where the particle(s) is not bound to a substrate.
    Type: Grant
    Filed: December 5, 2011
    Date of Patent: February 25, 2020
    Assignee: Cellply S.R.L.
    Inventors: Massimo Bocchi, Roberto Guerrieri
  • Patent number: 10564125
    Abstract: A method of forming a semiconductor structure includes forming a substrate, forming an anchor layer, and forming one or more self-aligned nanotip pillar pairs disposed vertically between the substrate and the anchor layer. A given one of the nanotip pillar pairs comprises a bottom nanotip pillar and a top nanotip pillar, the bottom nanotip pillar comprising a base portion disposed on a top surface of the substrate and the top nanotip pillar comprising a base portion disposed in the anchor layer. The bottom nanotip pillar and the top nanotip pillar comprise sidewalls that taper to points as distance from the respective base portions increases.
    Type: Grant
    Filed: December 14, 2017
    Date of Patent: February 18, 2020
    Assignee: International Business Machines Corporation
    Inventors: Juntao Li, Kangguo Cheng, Peng Xu, Heng Wu
  • Patent number: 10556234
    Abstract: An integrated system to isolate and diagnose circulating tumor cells (CTCs) within a cellular sample includes an isolating mechanism to isolate and trap large biological cells at a detection zone from among the cellular sample based on cells size, and includes a diagnosing mechanism to diagnose CTCs among the trapped large biological cells, based on cells electrical impedance.
    Type: Grant
    Filed: August 16, 2016
    Date of Patent: February 11, 2020
    Inventors: Mohammad Abdolahad, Seiedali Hosseini, Somayeh Zanganeh
  • Patent number: 10557150
    Abstract: In an illustrative embodiment, automated instruments comprising one or more flow-through electroporation devices or modules are provided to automate transformation of nucleic acids in live cells.
    Type: Grant
    Filed: September 14, 2019
    Date of Patent: February 11, 2020
    Assignee: Inscripta, Inc.
    Inventors: Jorge Bernate, Don Masquelier, Phillip Belgrader
  • Patent number: 10519037
    Abstract: An intermediate transfer surface includes a substrate, a two-dimensional array of electrodes, a dielectric spacer layer on the two-dimensional array of electrodes, and a voltage controller electrically connected to the array of electrodes. A method of manufacturing an intermediate transfer surface, depositing an array of etch stops on a conductive surface, etching the conductive surface to form mesas of the conductive surface separated by gaps, and coating the mesas with a dielectric coating. A microassembly system includes an assembly surface having a first two dimensional array of potential wells on a first surface, a first voltage source electrically connected to the first array of potential wells, an intermediate transfer surface having a second two dimensional array of potential wells on a second surface arranged to face the first surface, and a second voltage source electrically connected to the second array of potential wells.
    Type: Grant
    Filed: January 18, 2016
    Date of Patent: December 31, 2019
    Assignee: PALO ALTO RESEARCH CENTER INCORPORATED
    Inventors: Sourobh Raychaudhuri, Jeng Ping Lu, David K. Biegelsen
  • Patent number: 10495580
    Abstract: An inspection device (1) inspects an amount of dielectric particles contained in a sample liquid. The inspection device includes a dielectric collection unit (3), a pump unit (10) and an AC voltage supply unit (11). The dielectric collection unit includes at least one pair of electrodes (41, 42) and a flow channel (13) extending in a predetermined direction on the pair of electrodes. The pump unit is configured to feed the sample liquid to follow the flow channel in the predetermined direction. The AC voltage supply unit is configured to supply, to the pair of electrodes, an AC voltage with a predetermined frequency to cause dielectrophoresis for dielectric particles in the fed sample liquid. The dielectric collection unit includes a plurality of slit regions (Rs) aligned in the predetermined direction between the pair of electrodes. Each of the plurality of slit regions is separated from each other within the flow channel.
    Type: Grant
    Filed: October 5, 2016
    Date of Patent: December 3, 2019
    Assignee: AFI Corporation
    Inventors: Takaharu Enjoji, Yoshikazu Wakizaka, Satoshi Uchida, Eiko Kato, Masayo Takano
  • Patent number: 10478822
    Abstract: The present disclosure provides a reagent cartridge configured for use in an automated multi-module cell processing environment.
    Type: Grant
    Filed: July 29, 2019
    Date of Patent: November 19, 2019
    Assignee: Inscripta, Inc.
    Inventors: Don Masquelier, Phillip Belgrader, Brian Van Hatten, Jorge Bernate, Bruce Chabansky
  • Patent number: 10413912
    Abstract: Disclosed are apparatuses, systems, and methods for programmable fluidic processors. In one embodiment, the invention involves manipulating droplets across a reaction surface of the processor substantially contact-free of any surfaces. The reaction surface and the electrodes of the processor may include a coating repelling the droplets. Further, the present invention provides for a suitable suspending medium for repelling droplets away from fixed surfaces.
    Type: Grant
    Filed: March 2, 2015
    Date of Patent: September 17, 2019
    Assignee: THE BOARD OF REGENTS OF THE UNIVERSITY OF TEXAS SYSTEM
    Inventors: Peter R. C. Gascoyne, Jody Vykoukal, Jon Schwartz
  • Patent number: 10406525
    Abstract: The present disclosure provides a reagent cartridge configured for use in an automated multi-module cell processing environment.
    Type: Grant
    Filed: June 25, 2019
    Date of Patent: September 10, 2019
    Assignee: Inscripta, Inc.
    Inventors: Don Masquelier, Phillip Belgrader, Brian Van Hatten, Jorge Bernate, Bruce Chabansky
  • Patent number: 10384204
    Abstract: A system for operating an electrokinetic device includes a support configured to hold and operatively couple with the electrokinetic device, an integrated electrical signal generation subsystem configured to apply a biasing voltage across a pair of electrodes in the electrokinetic device, and a light modulating subsystem configured to emit structured light onto the electrokinetic device. The system can further include a thermally controlled flow controller, and/or be configured to measure impedance across the electrokinetic device. The system can be a light microscope, including an optical train. The system can further include a light pipe, which can be part of the light modulating subsystem, and which can be configured to supply light of substantially uniform intensity to the light modulating subsystem or directly to the optical train.
    Type: Grant
    Filed: December 9, 2015
    Date of Patent: August 20, 2019
    Assignee: Berkeley Lights, Inc.
    Inventors: Andrew W. McFarland, Daniele Malleo, J. Tanner Nevill, Russell A. Newstrom, Keith J. Breinlinger, Paul M. Lundquist, Justin K. Valley, Jonathan Cloud Dragon Hubbard
  • Patent number: 10376889
    Abstract: The present disclosure provides a reagent cartridge configured for use in an automated multi-module cell processing environment.
    Type: Grant
    Filed: April 10, 2019
    Date of Patent: August 13, 2019
    Assignee: Inscripta, Inc.
    Inventors: Don Masquelier, Phillip Belgrader, Brian Van Hatten, Jorge Bernate, Bruce Chabansky
  • Patent number: 10369565
    Abstract: Example methods, apparatus, systems for diluting samples are disclosed. An example method includes depositing a first fluid droplet on a first electrode of a plurality of electrodes. The first electrode has a first area. The first fluid droplet has a first volume associated with the first area. The example method includes depositing a second fluid droplet on a second electrode of the plurality of electrodes. The second electrode has a second area. The second fluid droplet has a second volume associated with the second area. The second volume is different than the first volume. The example method includes forming a combined droplet by selectively activating at least one of the first electrode or the second electrode to cause one of the first fluid droplet or the second fluid droplet to merge with the other of the first fluid droplet or the second fluid droplet.
    Type: Grant
    Filed: December 21, 2015
    Date of Patent: August 6, 2019
    Assignee: Abbott Laboratories
    Inventor: Andrew Fischer
  • Patent number: 10316363
    Abstract: Provided is a sensing apparatus comprising a chip for integrated amplification and sequencing of a template polynucleotide in a sample. The apparatus comprises a chip with at least one ISFET in a well or chamber, amplification means for amplifying the template polynucleotide on a surface of said chip and comprising at least one heating means suitable for conducting amplification of the template polynucleotide at temperatures elevated with respect to room temperature, and sequencing means for sequencing the amplified template polynucleotide in said well or chamber. Methods of use are also provided.
    Type: Grant
    Filed: April 24, 2017
    Date of Patent: June 11, 2019
    Assignee: DNAE Group Holdings Limited
    Inventors: Zahid Ansari, Krishna Amin, Ginny Jorgensen, Kurt Kolb, Daniel Morley, Alpesh Patel, Samuel Reed, Leila Shepherd, Christofer Toumazou
  • Patent number: 10307769
    Abstract: There is described herein methods and devices for confining and/or manipulating molecules. At least one molecule is introduced into a fluidic chamber. The fluidic chamber is formed inside a device comprising at least one first electrode having a first surface spaced from at least one second electrode having a second surface facing the first surface. The at least one second electrode has a plurality of dielectric structures arranged to form openings along the second surface. At least one electrical signal is applied across the at least one first electrode and the at least one second electrode to generate a non-uniform electric field having electric field lines extending from the first surface of the at least one first electrode to the second surface of the at least one second electrode in the openings formed between the dielectric structures. The at least one electrical signal has a frequency level causing the at least one molecule to move inside the fluidic chamber in accordance with a predetermined movement.
    Type: Grant
    Filed: May 16, 2017
    Date of Patent: June 4, 2019
    Assignee: THE ROYAL INSTITUTION FOR THE ADVANCEMENT OF LEARNING/MCGILL UNIVERSITY
    Inventors: Sara Mahshid, Mohammed Jalal Ahamed, Walter Reisner, Robert Sladek
  • Patent number: 10252269
    Abstract: Methods and devices are provided for focusing and/or sorting activated T cells. The device comprises a microfluidic channel comprising a plurality of electrodes arranged to provide dielectrophoretic (DEP) forces that are perpendicular to forces from hydrodynamic flows along the channel. The device may be configured to apply voltages to a plurality of electrodes in a first upper region of the microfluidic channel to focus the cells into a single flow, and to apply different voltages to a plurality of electrodes in a second downstream region of the microfluidic channel to sort cells based on size. The output of the microfluidic channel may diverge into multiple channels, wherein cells of various sorted sizes are directed into the appropriate output channel.
    Type: Grant
    Filed: November 17, 2017
    Date of Patent: April 9, 2019
    Assignees: NANTBIO, INC., NANOCAV, LLC
    Inventors: Yu-Chun Kung, Ting-Hsiang Wu, Peter Sieling, Kayvan Niazi
  • Patent number: 10208095
    Abstract: Non-centrifugal methods for generating a solution rich in interleukin-1 receptor antagonist from a tissue comprising cytokine-producing cells. The solution rich in IL-1ra can also include at least one of sTNF-RI, sTNF-RII, IGF-I, EGF, HGF, PDGF-AB, PDGF-BB, VEGF, TGF-?1, and sIL-1 RII.
    Type: Grant
    Filed: March 15, 2013
    Date of Patent: February 19, 2019
    Assignee: Biomet Manufacturing, LLC
    Inventors: Michael D. Leach, Jennifer E. Woodell-May, Joel C. Higgins, Krista O'Shaughnessey
  • Patent number: 10100418
    Abstract: According to one embodiment, an oxidation electrode includes: a collector; an oxidation catalyst formed on the collector; and a modified organic molecule which is bonded to the surface of the oxidation catalyst, and comprises a cationic functional group.
    Type: Grant
    Filed: September 9, 2016
    Date of Patent: October 16, 2018
    Assignee: KABUSHIKI KAISHA TOSHIBA
    Inventors: Jun Tamura, Satoshi Mikoshiba, Akihiko Ono, Yuki Kudo, Ryota Kitagawa, Chingchun Huang
  • Patent number: 10081015
    Abstract: A device for trapping at least one microparticle in a fluid flow is suggested. The device comprises a trapping element and an electrode. The trapping element is configured for trapping the at least one microparticle and has at least one recess for receiving the at least one microparticle. The electrode is configured for generating an asymmetric electric field. In operation, at least one microparticle of a plurality of microparticles passing through the asymmetric electric field is forced into the at least one recess of the trapping element.
    Type: Grant
    Filed: July 12, 2015
    Date of Patent: September 25, 2018
    Assignee: International Business Machines Corporation
    Inventors: Jaione Tirapu Azpiroz, Emmanuel Delamarche, Claudius Feger, Yuksel Temiz
  • Patent number: 10001449
    Abstract: Systems, methods, and test kits for detecting and quantifying an analyte level in a biological fluid sample using impedance measurements, are disclosed. The fluid sample is applied to a lateral flow strip, and impedance of the strip is measured as the assay dries. Analysis of the drying-dependent impedance measurements indicates the presence and quantity of the analyte in the fluid sample.
    Type: Grant
    Filed: December 15, 2014
    Date of Patent: June 19, 2018
    Assignee: Church & Dwight Co., Inc.
    Inventors: Albert Nazareth, Shang Li, Timothy Snowden, Giles H. W. Sanders, Anthony Cass
  • Patent number: 9983169
    Abstract: An integrated fluidic circuit has a supporting surface that carries a first fluid to be moved at a first functional region; a dielectric structure, defining the supporting surface; and an electrode structure, coupled to the dielectric structure for generating an electric field at the first functional region, such as to modify electrowetting properties of the interface between the first fluid and the supporting surface. The dielectric structure has a first spatially variable dielectric profile at the first functional region, thus determining a corresponding spatially variable profile of the electric field, and, consequently, of the electrowetting properties of the interface between the first fluid and the supporting surface. The integrated fluidic circuit may achieve mixing between the first fluid and a second fluid.
    Type: Grant
    Filed: September 17, 2014
    Date of Patent: May 29, 2018
    Assignee: STMICROELECTRONICS S.R.L.
    Inventor: Alessandro Paolo Bramanti
  • Patent number: 9981273
    Abstract: The procedure of dielectric electrophoresis (dielectrophoresis or DEP) utilizes field-polarized particles that move under the application of positive (attractive) and/or negative (repulsive) applied forces. This invention uses negative dielectric electrophoresis (negative dielectrophoresis or nDEP) within a microchannel separation apparatus to make particles move (detached) or remain stationary (attached). In an embodiment of the present invention, the nDEP force generated was strong enough to detach Ag-Ab (antigen-antibody) bonds, which are in the order of 400 pN (piconewtons) while maintaining the integrity of the system components.
    Type: Grant
    Filed: September 30, 2013
    Date of Patent: May 29, 2018
    Assignee: The Board of Trustees of the Leland Stanford Junior University
    Inventors: Mehdi Javanmard, Sam Emaminejad, Janine Mok, Michael N. Mindrinos
  • Patent number: 9962714
    Abstract: A microchannel for processing microparticles in a fluid flow comprises a first and second pairs of electrodes. The first pair of electrodes is configured for generating an asymmetric first electric field and for sorting the microparticles to provide sorted microparticles. The second pair of electrodes is configured for generating an asymmetric second electric field and for trapping at least some of the sorted microparticles.
    Type: Grant
    Filed: July 12, 2015
    Date of Patent: May 8, 2018
    Assignee: INTERNATIONAL BUSINESS MACHINES CORPORATION
    Inventors: Jaione Tirapu Azpiroz, Emmanuel Delamarche, Claudius Feger, Yuksel Temiz
  • Patent number: 9915599
    Abstract: There is provided a microparticle analysis apparatus including a sample channel configured to receive liquid containing a plurality of microparticles, a first pair of electrodes configured to form an alternating electric field in at least a part of the sample channel, a measuring part configured to measure impedance between the first pair of electrodes, an analyzing part configured to calculate property values of the microparticles from the impedance measured in the measuring part, and a determining part configured to determine whether data of the impedance measured in the measuring part is derived from the microparticles.
    Type: Grant
    Filed: December 26, 2013
    Date of Patent: March 13, 2018
    Assignee: Sony Corporation
    Inventors: Marcaurele Brun, Kazumasa Sato, Shinji Omori, Yoichi Katsumoto
  • Patent number: 9891157
    Abstract: An inverted microwell (102) provides rapid and efficient microanalysis system (100) and method for screening of biological particles (128), particularly functional analysis of cells on a single cell basis. The use of an inverted open microwell system (102) permits identification of particles, cells, and biomolecules that may be combined to produce a desired functional effect also functional screening of secreted antibody therapeutic activity as well as the potential to recover cells and fluid, and optionally expand cells, such as antibody secreting cells, within the same microwell.
    Type: Grant
    Filed: December 5, 2011
    Date of Patent: February 13, 2018
    Assignee: CELLPLY S.R.L.
    Inventors: Massimo Bocchi, Roberto Guerrieri
  • Patent number: 9879354
    Abstract: The present invention discloses an electrochemical process for water splitting for production of oxygen using porous Co3O4 nanorods with a considerably low overpotential and high exchange current density. The present invention further discloses a simple, industrially feasible process of for preparation of said nanostructured porous cobalt oxide catalyst thereof.
    Type: Grant
    Filed: April 25, 2013
    Date of Patent: January 30, 2018
    Assignee: Council of Scientific and Industrial Research
    Inventors: Pattayil Alias Joy, Vijayamohanan Kunjukrishna Pillai, Ramasundar Mohan Rani, Joyashish Debgupta
  • Patent number: 9861979
    Abstract: The present invention generally pertains to a system for performing injection of multiple substantially controlled volumes into or out of a droplet, and methods and kits comprising the same. The system of the present invention comprises at least one microfluidic channel, one or more injection channels, an injection inlet associated with each of the one or more injection channels, and a mechanism for disrupting an interface between a droplet and a fluid and/or emulsion, wherein the at least one microfluidic channel comprises one or more droplets are flowing therein, and wherein each of the one or more injection channels comprises at least one fluid and/or emulsion therein.
    Type: Grant
    Filed: March 28, 2012
    Date of Patent: January 9, 2018
    Assignee: Bio-Rad Laboratories, Inc.
    Inventors: Adam Abate, Sepehr Kiani, Tony Hung, Pascaline Mary, Adnan Moez Esmail
  • Patent number: 9846150
    Abstract: A particle separating apparatus and method are provided, which pass a fluid sample such as blood through a filter to remove foreign matter, and separate target particles by using a MOFF channel, and re-separate the separated target particles through dielectrophoresis. The particle separating apparatus includes a MOFF (Multi Orifice Flow Fractionation) channel including a multi orifice segment through which a fluid sample passes to discharge a primarily separated material that are target particles separated from the fluid sample, through a central passage; a dielectrophoresis channel including a pair of electrodes to which AC power is applied and forming an electric field in a flow channel connected to the central passage of the MOFF channel to re-separate the target particles from the primarily separated material discharged from the central passage of the MOFF channel through dielectrophoresis.
    Type: Grant
    Filed: August 8, 2011
    Date of Patent: December 19, 2017
    Assignee: INDUSTRY-ACADEMIC COOPERATION FOUNDATION YONSEI UNIVERSITY
    Inventors: Hyo Il Jung, Ki Ho Kwon, Hui Sung Moon, Joo Hyuk Sohn, Seung Il Kim
  • Patent number: 9816988
    Abstract: The present disclosure provides apparatuses and methods for analyzing the presence of a target analyte. The apparatuses and methods of the present disclosure can be operated in a multiplexed format to perform various assays of clinical significance.
    Type: Grant
    Filed: March 6, 2017
    Date of Patent: November 14, 2017
    Assignee: MULTERRA BIO, INC.
    Inventors: Kee-Hyun Paik, Kaveh M. Milaninia, Kilian Dill
  • Patent number: 9779189
    Abstract: A computerized method for designing a discrete droplet microfluidic system: (a) provides an initial set of droplet based networks; (b) codes each droplet based network into a data structure such that all the data structures form a current set of data structures; (c) creates new data structures by performing one or more genetic operators on the current set of data structures; (d) adds new data structures to the current set of data structures; (e) creates a new set of data structures that satisfies one or more design parameters; (f) replaces the current set of data structures with the new set of data structures; (g) repeats steps (c), (d), (e) and (f) until the new set of data structures has been created a third number of times; and (h) displays/outputs the current set of data structures as possible designs for the discrete droplet microfluidic system to one or more output devices.
    Type: Grant
    Filed: July 11, 2014
    Date of Patent: October 3, 2017
    Assignee: Texas Tech University System
    Inventors: Jeevan Maddala, Raghunathan Rengaswamy
  • Patent number: 9759683
    Abstract: A miniaturized electrophoresis device with integrated electrochemical detection for detecting target molecules by electrochemical separation. The miniaturized electrophoresis device with integrated electrochemical detection includes a planar member having a top side and made of an inert substrate; and unit cells integrated and adjacently arranged consecutively upon the top side of the planar member and connectable to a power source to effect an electric potential across the unit cells to separate ionic target molecules from a solution deposited upon the planar member increasing signal to noise ratio.
    Type: Grant
    Filed: May 12, 2015
    Date of Patent: September 12, 2017
    Inventor: Stephen D. House
  • Patent number: 9757737
    Abstract: A cell sorting apparatus includes a flow channel through which fluid including cells flows, an electric-field application section capable of applying an electric field having a gradient in a direction different from the flowing direction of the fluid at a first position on the flow channel in accordance with a cell sorting signal requesting an operation to sort the cells, and a flow splitting section configured to split the cells changing their flowing directions due to a dielectrophoretic force caused by application of the electric field at a second position on the downstream side of the first position on the flow channel.
    Type: Grant
    Filed: October 13, 2015
    Date of Patent: September 12, 2017
    Assignee: Sony Corporation
    Inventors: Yoichi Katsumoto, Kazumasa Sato, Heloise Cockenpot
  • Patent number: 9731293
    Abstract: The combined value of integrating optical forces and electrokinetics allows for the pooled separation vectors of each to be applied, providing for separation based on combinations of features such as size, shape, refractive index, charge, charge distribution, charge mobility, permittivity, and deformability. The interplay of these separation vectors allow for the selective manipulation of analytes with a finer degree of variation. Embodiments include methods of method of separating particles in a microfluidic channel using a device comprising a microfluidic channel, a source of laser light focused by an optic into the microfluidic channel, and a source of electrical field operationally connected to the microfluidic channel via electrodes so that the laser light and the electrical field to act jointly on the particles in the microfluidic channel. Other devices and methods are disclosed.
    Type: Grant
    Filed: October 1, 2013
    Date of Patent: August 15, 2017
    Assignee: The United States of America, as represented by the Secretary of the Navy
    Inventors: Alexander V. Terray, Sean J. Hart, Sarah J. R. Staton, Gregory E. Collins
  • Patent number: 9580742
    Abstract: Contemplated methods and devices are drawn to preparation and analysis of analytes from biological samples. In a preferred embodiment the analytes are nucleic acids that are both released from biological compartment present in the sample and fragmented through the use of a voltage potential applied to a pair of electrodes. The nucleic acids thus prepared are subsequently characterized.
    Type: Grant
    Filed: March 12, 2012
    Date of Patent: February 28, 2017
    Inventors: Shana O. Kelley, Susan Bortolin, Reginald James McKenzie Orton, Stefan Christopher Wiechula
  • Patent number: 9566558
    Abstract: The present invention concerns a microfluidic device (1) for performing physical, chemical or biological treatment to at least one packet without contamination.
    Type: Grant
    Filed: September 9, 2005
    Date of Patent: February 14, 2017
    Assignees: INSTITUT CURIE, CENTRE NATIONAL DE RECHERCHE SCIENTIFIQUE
    Inventors: Jean-Louis Viovy, Max Chabert, Kevin Dorfman
  • Patent number: 9555421
    Abstract: Provided is a sorting apparatus including: a flow channel device including a flow channel through which a fluid including particles flows and an operation electrode portion that causes a dielectrophoretic force to act on the particles in the flow channel; and a controller configured to detect characteristics of the particles flowing through the flow channel, generate a voltage signal by a pulse modulation using a square pulse based on the detected characteristics of the particles, and output the voltage signal to the operation electrode portion.
    Type: Grant
    Filed: January 23, 2014
    Date of Patent: January 31, 2017
    Assignee: SONY CORPORATION
    Inventors: Kazumasa Sato, Yoichi Katsumoto
  • Patent number: 9511368
    Abstract: A device for transporting, trapping and escaping a single biomaterial using a magnetic structure, and a method of transporting, trapping and escaping of the single biomaterial using the same are provided, and a method is provided for controlling movement and direction of the single biomaterial including soft magnetic micro structure and magnetic structure in a linear, square storage, apartment type, radial soft magnetic micro structure. Accordingly, the device for transporting, trapping and escaping a single biomaterial and the method for transporting, trapping and escaping single biomaterial using the same can control movement on the lap-on-a-chip with increased precision and ease, by using magnetic force, and thus can be advantageously used in the field of magneto-resistive sensor, or categorization of single cells or biomolecules.
    Type: Grant
    Filed: August 29, 2013
    Date of Patent: December 6, 2016
    Assignee: The Industry & Academic Cooperation in Chungnam National University (IAC)
    Inventors: Cheolgi Kim, Byeonghwa Lim, Venu Reddy, XingHao Hu, KunWoo Kim, Benjamin B. Yellen
  • Patent number: 9506936
    Abstract: The invention pertains to a method which allows separation of nucleated fetal cells, particularly fetal erythroblasts, from maternal peripheral blood. More specifically the invention relates to a non-invasive method which can isolate and provide intact nucleated fetal cells, and is useful for subsequent chromosome, gene expression and protein investigations, and is feasible at ail gestational ages.
    Type: Grant
    Filed: April 24, 2014
    Date of Patent: November 29, 2016
    Assignee: FONDAZIONE IRCCS CA' GRANDA OSPEDALE MAGGIORE POLICLINICO
    Inventor: Debora Lattuada
  • Patent number: 9499812
    Abstract: The present invention includes methods, devices and systems for isolating a nucleic acid from a fluid comprising cells. In various aspects, the methods, devices and systems may allow for a rapid procedure that requires a minimal amount of material and/or results in high purity nucleic acid isolated from complex fluids such as blood or environmental samples.
    Type: Grant
    Filed: October 28, 2015
    Date of Patent: November 22, 2016
    Assignee: BIOLOGICAL DYNAMICS, INC.
    Inventors: Rajaram Krishnan, David Charlot, Eugene Tu, James McCanna, Lucas Kumosa, Paul Swanson, Robert Turner, Kai Yang, Irina Dobrovolskaya, David Liu
  • Patent number: 9480992
    Abstract: The invention relates to a method for the separation of a polarizable bioparticle comprising the steps: a) dielectrophoretic preseparation of a polarizable bioparticle from a suspension of bioparticles; b) fluidic separation of the selected bioparticle by fixing the bioparticle in a dielectrophoretic field cage and circulating fluid around the bioparticle; c) transferring the separated bioparticle from the dielectrophoretic field cage to a culture chamber; d) dielectrophoretic fixing of the separated bioparticle in the culture chamber and study, observation, manipulation and/or culturing of the separated bioparticle. The invention further relates to a microfluidic system and use thereof.
    Type: Grant
    Filed: May 9, 2012
    Date of Patent: November 1, 2016
    Assignee: Technische Universitat Dortmund
    Inventors: Andreas Schmid, Lars M. Blank, Steffen Howitz, Frederik Sven Ole Fritzsch
  • Patent number: 9433941
    Abstract: In one implementation, a microfluidic device based on optical trapping of particles is disclosed to include a substrate structured to include a fluidic channel which can carry a fluid having particles; and an optical waveguide loop formed on the substrate to include one or more waveguide sections that reside within the fluidic channel, an input optical port for the optical waveguide to receive an input optical beam, and an optical power splitter coupled to the optical waveguide loop to split the received input optical beam into two counter-propagating optical beams that prorogate in the optical waveguide loop in opposite directions and interfere with each other to form standing optical waves in at least the one or more waveguide sections that reside within the fluidic channel to optically trap particles at or near a surface of the one or more waveguide sections that reside within the fluidic channel.
    Type: Grant
    Filed: December 23, 2013
    Date of Patent: September 6, 2016
    Assignee: CORNELL UNIVERSITY
    Inventors: Michelle D. Wang, Michal Lipson, Mohammad Soltani, Jun Lin, Summer N. Saraf