Dielectrophoresis (i.e., Using Nonuniform Electric Field) Patents (Class 204/547)
  • Patent number: 11291970
    Abstract: A gene sequencing chip is provided, which includes: an upper substrate including a plurality of liquid inlets for inletting liquid drops; a lower substrate opposite to the upper substrate and spaced therefrom by a gap, the gap being provided for allowing the liquid drops to move therein, the lower substrate including a liquid drop operation region, the liquid drop operation region including a manipulation electrode array. The manipulation electrode array includes multiple first sub-arrays for preparing a gene library, multiple second sub-arrays for sequencing the gene library which is prepared, each first sub-array being adjacent to one of the multiple second sub-arrays. Based on the gene sequencing chip provided in this disclosure, operations to tiny liquid drops such as movement, fusion and splitting can be accurately manipulated by using digital microfluidic techniques, and all steps of the gene sequencing from library preparation to gene sequencing can be completed on one chip.
    Type: Grant
    Filed: January 2, 2018
    Date of Patent: April 5, 2022
    Assignees: BOE TECHNOLOGY GROUP CO., LTD., BEIJING BOE OPTOELECTRONICS TECHNOLOGY CO., LTD.
    Inventors: Yue Geng, Fengchun Pang, Peizhi Cai, Le Gu
  • Patent number: 11237161
    Abstract: Devices, systems, and methods for detecting molecules of interest within a collected sample are described herein. In certain embodiments, self-contained sample analysis systems are disclosed, which include a reusable reader component, a disposable cartridge component, and a disposable sample collection component. The reader component may communicate with a remote computing device for the digital transmission of test protocols and test results. In various disclosed embodiments, the systems, components, and methods are configured to identify the presence, absence, and/or quantity of particular nucleic acids, proteins, or other analytes of interest, for example, in order to test for the presence of one or more pathogens or contaminants in a sample.
    Type: Grant
    Filed: January 24, 2018
    Date of Patent: February 1, 2022
    Assignee: CUE HEALTH INC.
    Inventor: Ayub Khattak
  • Patent number: 11203734
    Abstract: Methods and apparatus for detecting, quantifying, enriching, and/or separating bacterial species in fluid sample are provided. The fluid sample is provided as input to a microfluidic passage of a microfluidic device, wherein the microfluidic device comprises at least one electrode disposed adjacent to the microfluidic passage. The at least one electrode is activated to capture bacteria in the sample using dielectrophoresis, wherein the capture efficiency of bacteria is at least 99%.
    Type: Grant
    Filed: December 31, 2020
    Date of Patent: December 21, 2021
    Assignee: Fluid-Screen, Inc.
    Inventors: Monika Weber, Slawomir Antoszczyk, Robert Weber
  • Patent number: 11198844
    Abstract: Methods and apparatus for detecting, quantifying, enriching, and/or separating bacterial species in fluid sample are provided. The fluid sample is provided as input to a microfluidic passage of a microfluidic device, wherein the microfluidic device comprises at least one electrode disposed adjacent to the microfluidic passage. The at least one electrode is activated to capture bacteria in the sample using dielectrophoresis, wherein the capture efficiency of bacteria is at least 99%.
    Type: Grant
    Filed: December 31, 2020
    Date of Patent: December 14, 2021
    Assignee: Fluid-Screen, Inc.
    Inventors: Monika Weber, Slawomir Antoszczyk, Robert Weber
  • Patent number: 11198140
    Abstract: Provided is a concentration device suitable for dielectrophoresis. The concentration device comprises a first substrate, a second substrate provided so as to face the first substrate, a flow path formed between the first substrate and the second substrate, a first pillar electrode line disposed in the flow path and including a left-side first pillar electrode L (301L), a right-side first pillar electrode R (301R), and one second pillar electrode B (302B), and a second pillar electrode line disposed in the flow path and including one second pillar electrode A (302A). The value of L3 is not less than 5 micrometers, where L3 is equal to (A1?A2), A1 represents a distance between a second vertex Q2 of the second pillar electrode A and a center point O; and A2 represents a distance between the first vertex Q1 of the second pillar electrode B and the center point O.
    Type: Grant
    Filed: January 8, 2019
    Date of Patent: December 14, 2021
    Assignee: PANASONIC INTELLECTUAL PROPERTY MANAGEMENT CO., LTD.
    Inventors: Noriko Shimba, Kazuaki Nishio
  • Patent number: 11198902
    Abstract: Methods and apparatus for the selection or processing of particles sensitive to the application of an external stimulus to rupture/lysis at least one selected particle or the fusion of first and second selected particles are disclosed herein. Particles are organized using a first field of force by selectively energizing electrodes of an array of selectable electrodes having dimensions comparable to or smaller than those of the particles. A first configuration of stresses is applied to the electrodes; and then a second configuration of stresses is applied to the electrodes, so as to create a second field of force, located substantially close to at least one selected particle to be lysated or to a pair of first and second particles to be fused and such as to produce the application of a stimulus suited to produce their lysis or fusion.
    Type: Grant
    Filed: November 28, 2018
    Date of Patent: December 14, 2021
    Assignee: Menarini Silicon Biosystems S.p.A.
    Inventors: Nicolò Manaresi, Gianni Medoro, Mélanie Abonnenc
  • Patent number: 11192107
    Abstract: A microfluidic apparatus can comprise a dielectrophoresis (DEP) configured section for holding a first liquid medium and selectively inducing net DEP forces in the first liquid medium. The microfluidic apparatus can also comprise an electrowetting (EW) configured section for holding a second liquid medium on an electrowetting surface and selectively changing an effective wetting property of the electrowetting surface. The DEP configured section can be utilized to select and move a micro-object in the first liquid medium. The EW configured section can be utilized to pull a droplet of the first liquid medium into the second liquid medium.
    Type: Grant
    Filed: April 25, 2015
    Date of Patent: December 7, 2021
    Assignee: Berkeley Lights, Inc.
    Inventors: Igor Y. Khandros, J. Tanner Nevill, Steven W. Short, Ming C. Wu
  • Patent number: 11187986
    Abstract: Apparatuses and methods are described for removing edge bead on a wafer associated with a resist coating comprising a metal containing resist compositions. The methods can comprise applying a first bead edge rinse solution along a wafer edge following spin coating of the wafer with the metal based resist composition, wherein the edge bead solution comprises an organic solvent and an additive comprising a carboxylic acid, an inorganic fluorinated acid, a tetraalkylammonium compound, or a mixture thereof. Alternatively or additionally, the methods can comprise applying a protective composition to the wafer prior to performing an edge bead rinse. The protective composition can be a sacrificial material or an anti-adhesion material and can be applied only to the wafer edge or across the entire wafer in the case of the protective composition. Corresponding apparatuses for processing the wafers using these methods are presented.
    Type: Grant
    Filed: March 6, 2020
    Date of Patent: November 30, 2021
    Assignee: Inpria Corporation
    Inventors: Mollie Waller, Brian J. Cardineau, Kai Jiang, Alan J. Telecky, Stephen T. Meyers, Benjamin L. Clark
  • Patent number: 11154863
    Abstract: A system for processing a sample includes a chamber having at least one inlet and at least one outlet, where the chamber is configured to accommodate flow of the sample from the at least one inlet toward the at least one outlet, and an imager array configured to image the flow of the sample in the chamber, where the imager array includes at least one lensless image sensor configurable opposite at least one light source.
    Type: Grant
    Filed: October 8, 2019
    Date of Patent: October 26, 2021
    Assignee: Bioelectronica Corporation
    Inventors: Leonard Sherman, Roger Chen, Jonathan F. Hull
  • Patent number: 11117133
    Abstract: A microfluidic system which enables singular confinement of cells at the capturing stations and impedance measurements of single cells at these stations. The microfluidic system includes an inlet, a dielectrophoretic separation site, a waste outlet I, a connection pad, a hydrodynamic flow resistance. Collective measurements can also be obtained by measuring up to twenty singular cells at capturing stations simultaneously.
    Type: Grant
    Filed: August 17, 2018
    Date of Patent: September 14, 2021
    Assignee: ISTANBUL TEKNIK UNIVERSITESI
    Inventors: Huseyin Kizil, Emre Altinagac
  • Patent number: 11072810
    Abstract: Systems and methods are provided herein for rapid detection, separation, purification, and quantification of viral particles in a sample. According to some embodiments, a microfluidic device is provided for receiving the sample which may contain viral particles. An electrode of the microfluidic device may be used to generate dielectrophoretic (DEP) and/or electroosmotic (EO) forces acting on the sample. The applied DEP and/or EO forces may immobilize components of the sample on the surface of the electrode, may aggregate viral particles of the sample in one region of the microfluidic device, and may separate other components of the sample from the viral particles. The techniques may be performed rapidly, for example, in eight hours or less, and may not affect infectivity of the viral particles. In some embodiments, the sample may be labeled to enhance a response of one or more of the sample components to the DEP and/or EO forces.
    Type: Grant
    Filed: November 12, 2020
    Date of Patent: July 27, 2021
    Assignee: Fluid-Screen, Inc.
    Inventors: Slawomir Antoszczyk, Monika Weber, Robert Weber
  • Patent number: 11067535
    Abstract: Provided are a fluorescent testing system, a dielectrophoresis device, and a molecular testing method that measure only fluorescence emitted from a test object without separating excitation light and the fluorescence by an optical filter and that are able to prevent reduction of an application range of a type of the fluorescence.
    Type: Grant
    Filed: August 2, 2017
    Date of Patent: July 20, 2021
    Assignees: SHARP KABUSHIKI KAISHA, The University of Tokyo
    Inventors: Kunihiko Iizuka, Yoshihisa Fujimoto, Takeshi Mitsunaka, Soo-Hyeon Kim, Teruo Fujii
  • Patent number: 11059052
    Abstract: A method for dielectrophoresis includes applying an electric field across a micro-fluidic chamber with an alternating current (AC), trapping the target particles on the at least one carrier particle, transporting the target particles from a first location in the chamber to a second location in the chamber distanced from the first location with the at least one carrier particle and dynamically controlling the trapping and the transporting based on remotely applying forces on the at least one carrier particle. The trapping is based on localized gradients of the electric field induced by the carrier particle. The applied electric field is uniform absent a carrier particle present in the micro-fluidic chamber. The micro-fluidic chamber contains an electrolyte-solution with suspended target particles and at least one carrier particle freely floating on or in the electrolyte-solution.
    Type: Grant
    Filed: June 2, 2017
    Date of Patent: July 13, 2021
    Assignee: Technion Research & Development Foundation Limited
    Inventors: Gilad Yossifon, Alicia Boymelgreen
  • Patent number: 11042085
    Abstract: Provided are a pellicle film, a pellicle frame and a pellicle having a higher EUV transmittance. An exposure pattern plate capable of performing EUV lithography with the pellicle film, the pellicle frame or the pellicle, and a method for producing a semiconductor device, are provided. A pellicle film for exposure extendable over an opening of a support frame and having a thickness of 200 nm or less is provided. The film includes a carbon nanotube sheet. The carbon nanotube sheet includes bundles each including a plurality of carbon nanotubes, the bundles each have a diameter of 100 nm or shorter, and the bundles are aligned in a planar direction in the carbon nanotube sheet.
    Type: Grant
    Filed: December 18, 2018
    Date of Patent: June 22, 2021
    Assignees: MITSUI CHEMICALS, INC., NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCE AND TECHNOLOGY
    Inventors: Yosuke Ono, Atsushi Okubo, Kazuo Kohmura, Atsuko Sekiguchi, Yuichi Kato, Takeo Yamada
  • Patent number: 11040345
    Abstract: An embodiment of the present invention provides a microfluidic device into which fluid can be more easily introduced. A microfluidic device (1) is configured such that: (i) an upper substrate (2) is bonded to a lower substrate (6) via a sealing pattern (5) in such a manner that at least a portion of an edge of the upper substrate (2) is located inward of an edge of the lower substrate (6); and (ii) the sealing pattern (5) includes at least one gap (12) that is provided at a position where the edge of the upper substrate (2) is located inward of the edge of the lower substrate (6).
    Type: Grant
    Filed: March 23, 2017
    Date of Patent: June 22, 2021
    Assignee: Sharp Life Science (EU) Limited
    Inventors: Tomoko Teranishi, Yunting Shen, Hao Li
  • Patent number: 11003111
    Abstract: An example apparatus includes a conveyor. The apparatus also includes a first developer unit. The first developer unit is to concentrate first printing liquid. The first developer unit also is to deliver the first printing liquid to the conveyor. The apparatus also includes a second developer unit. The second developer unit is to concentrate second printing liquid. The second developer also is to deliver the second printing liquid to the conveyor to form a thick layer of printing liquid comprising the first and second printing liquid.
    Type: Grant
    Filed: December 2, 2019
    Date of Patent: May 11, 2021
    Assignee: HP Indigo B.V.
    Inventors: Giries Kadis, Marc Klein, Mark Sandler, Peter Nedelin, Marko Richter
  • Patent number: 10990001
    Abstract: Provided are a pellicle film, a pellicle frame and a pellicle having a higher EUV transmittance. An exposure pattern plate capable of performing EUV lithography with the pellicle film, the pellicle frame or the pellicle, and a method for producing a semiconductor device, are provided. A pellicle film for exposure extendable over an opening of a support frame and having a thickness of 200 nm or less is provided. The film includes a carbon nanotube sheet. The carbon nanotube sheet includes bundles each including a plurality of carbon nanotubes, the bundles each have a diameter of 100 nm or shorter, and the bundles are aligned in a planar direction in the carbon nanotube sheet.
    Type: Grant
    Filed: December 18, 2018
    Date of Patent: April 27, 2021
    Assignees: MITSUI CHEMICALS, INC., NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCE AND TECHNOLOGY
    Inventors: Yosuke Ono, Atsushi Okubo, Kazuo Kohmura, Atsuko Sekiguchi, Yuichi Kato, Takeo Yamada
  • Patent number: 10895575
    Abstract: The present invention relates to a method for the diagnosis of tumoural conditions and/or of the corresponding state of advance, wherein a sample from a patient comprising at least one tumour cell is obtained. According to the invention, a purified specimen of the at least one tumour cell is obtained by individually selecting and isolating single cells in a microfluidic device the purified specimen having a purity of at least 90%. On the purified specimen thus obtained there is subsequently performed a molecular analysis such as to highlight a characteristic thereof suited to enabling diagnosis.
    Type: Grant
    Filed: November 14, 2014
    Date of Patent: January 19, 2021
    Assignee: Menarini Silicon Biosystems S.p.A.
    Inventors: Nicolò Manaresi, Gianni Medoro, Giuseppe Giorgini
  • Patent number: 10888875
    Abstract: Devices, systems, and methods for applying a dielectrophoretic force on a particle include: a cell defining at least one channel for confining the particle; and a first electrode and a second electrode electrically isolated from the first electrode, at least one of the first and second electrodes being formed from a two-dimensional (2D) material providing an atomically sharp edge. The first and second electrodes are arranged sufficiently close to one another and sufficiently close to the channel such that application of a sufficient voltage across the first and second electrodes generates an electric field in at least part of the channel, the electric field having an electric field gradient sufficient to apply the dielectrophoretic force on the particle in the channel.
    Type: Grant
    Filed: June 18, 2018
    Date of Patent: January 12, 2021
    Assignee: Regents of the University of Minnesota
    Inventors: Sang-Hyun Oh, Steven John Koester
  • Patent number: 10821439
    Abstract: A microfluidic apparatus is provided having one or more sequestration pens configured to isolate one or more target micro-objects by changing the orientation of the microfluidic apparatus with respect to a globally active force, such as gravity. Methods of selectively directing the movements of micro-objects in such a microfluidic apparatus using gravitational forces are also provided. The micro-objects can be biological micro-objects, such as cells, or inanimate micro-objects, such as beads.
    Type: Grant
    Filed: July 26, 2017
    Date of Patent: November 3, 2020
    Assignee: Berkeley Lights, Inc.
    Inventors: Keith J. Breinlinger, Eric D. Hobbs, Daniele Malleo, J. Tanner Nevill, Mark P. White
  • Patent number: 10813688
    Abstract: A method for treating Chronic Obstructive Pulmonary Disease (COPD) or chronic bronchitis to alleviate the discomforts of breathing by using non-thermal electroporation energy to ablate diseased portions of the lung including the bronchus, airways and alveoli which, in effect, opens the restrictive diseased portions thereby maximizing the overall surface area thereof causing improved airflow and uninhibited breathing.
    Type: Grant
    Filed: November 23, 2015
    Date of Patent: October 27, 2020
    Assignee: AngioDynamics, Inc.
    Inventors: Robert Pearson, Mark Ortiz, Peter Callas
  • Patent number: 10816536
    Abstract: A bioparticle observation apparatus includes a dielectrophoresis electrode that outputs a first signal causing a dielectrophoresis force to act on a bioparticle, a sensor electrode that detects an impedance difference between the bioparticle and the liquid, and a control circuit that controls the first signal so that the detected impedance difference is fixed.
    Type: Grant
    Filed: June 10, 2019
    Date of Patent: October 27, 2020
    Assignees: SHARP KABUSHIKI KAISHA, KYOTO UNIVERSITY
    Inventors: Takeshi Mitsunaka, Yuichi Ogawa
  • Patent number: 10780444
    Abstract: A system and method for detection of cells is disclosed. Target cells, such as circulating tumor cells (CTCs), may be of interest. Magnetic beads may be bound to the target cells. After which, the target cells (with the magnetic beads attached thereto) may be identified using an applied magnetic field. In one example, magnetic sensors may be used to detect movement of the target cells responsive to an applied magnetic field. In another example, an optical sensor (such as a camera) may be used to detect movement of the target cells responsive to an applied magnetic field. Further, separate from identification of the target cells, the target cells may be sorted using an applied magnetic field. In this way, a magnetic field may be used in either or both of target cell identification or target cell sorting in order to detect target cells of interest.
    Type: Grant
    Filed: January 4, 2018
    Date of Patent: September 22, 2020
    Assignee: Microsensor Labs, LLC
    Inventor: Peng Liu
  • Patent number: 10722887
    Abstract: Method, apparatus, and computer program product for a microfluidic channel having a cover opposite its bottom, the cover allowing visual inspection inside the channel, and having electrodes with patterned planar conducting materials, integrated onto its bottom. Using the planar conducting materials, once a fluid sample with suspended microparticles is applied into the channel, highly localized modulated electric field distributions are generated inside the channel and the fluid sample. This generated field causes inducing of dielectrophoretic (DEP) forces such that the DEP forces gradually increase along the length of the channel occupied by the electrodes. These DEP forces counteract the hydrodynamic drag of the flow acting on the particles suspended in the fluid. Because of the induced forces, micro/nano-particles in the fluid sample are deflected at locations in the microchannel that are a function of the particles velocity and this effect is captured by an image sensing device.
    Type: Grant
    Filed: March 21, 2017
    Date of Patent: July 28, 2020
    Assignee: International Business Machines Corporation
    Inventors: Jaione Tirapu Azpiroz, Emmanuel Delamarche, Yuksel Temiz
  • Patent number: 10705011
    Abstract: A method for dynamic focusing is presented that can be performed by a dynamic focusing controller that can receive images from the image capture device, and for each image, determine a border of the particle within the image, and calculate a pixel intensity ratio of the image based on the border of the particle. The dynamic focusing controller can also calculate a median pixel intensity ratio from the pixel intensity ratios for each image, determine a focal distance direction based on the median pixel intensity ratio, calculate a focal distance based on the median pixel intensity ratio when the focal distance direction is positive, and calculate the focal distance based on a median border width when the focal distance direction is negative. The autofocusing controller can then send an instruction to the focusing mechanism to adjust the image capture device by the focal distance in the focal distance direction.
    Type: Grant
    Filed: October 5, 2017
    Date of Patent: July 7, 2020
    Assignee: Beckman Coulter, Inc.
    Inventors: Jiuliu Lu, Bian Qian, Bart J. Wanders, Ken Good, John Riley
  • Patent number: 10684254
    Abstract: The present invention provides a method for detecting an analyte with high sensitivity. In the present method, a solution is supplied onto a substrate comprising a first electrode and a second electrode. Then, an alternating voltage is applied between the first electrode and the second electrode to aggregate, onto the surface between the first electrode and the second electrode by dielectrophoresis, bioparticles and dielectric particles contained in the solution. The aggregated bioparticles are broken to release the analyte contained in the bioparticles. The released analyte is bound to a first antibody and a second antibody to cause the dielectric particles to be immobilized onto the substrate through formation of a sandwich structure composed of the first antibody, the analyte, and the second antibody. Finally, the analyte is detected through the fluorescent substance contained in the immobilized dielectric particles.
    Type: Grant
    Filed: December 1, 2016
    Date of Patent: June 16, 2020
    Assignee: Panasonic Intellectual Property Management Co., Ltd.
    Inventors: Yasuaki Okumura, Tatsurou Kawamura, Noriko Shimba
  • Patent number: 10627719
    Abstract: Methods are described for removing edge bead on a wafer associated with a resist coating comprising a metal containing resist compositions. The methods can comprise applying a first bead edge rinse solution along a wafer edge following spin coating of the wafer with the metal based resist composition, wherein the edge bead solution comprises an organic solvent and an additive comprising a carboxylic acid, an inorganic fluorinated acid, a tetraalkylammonium compound, or a mixture thereof. Alternatively or additionally, the methods can comprise applying a protective composition to the wafer prior to performing an edge bead rinse. The protective composition can be a sacrificial material or an anti-adhesion material and can be applied only to the wafer edge or across the entire wafer in the case of the protective composition. Corresponding apparatuses for processing the wafers using these methods are presented.
    Type: Grant
    Filed: August 11, 2017
    Date of Patent: April 21, 2020
    Assignee: Inpria Corporation
    Inventors: Mollie Waller, Brian J. Cardineau, Kai Jiang, Alan J. Telecky, Stephen T. Meyers, Benjamin L. Clark
  • Patent number: 10612155
    Abstract: Provided are a protein crystal device and method for crystallizing protein capable of generating protein crystal without imparting a heat effect, a protein crystal-cutting device and method for cutting protein crystal capable of cutting protein crystal without imparting a heat effect on protein crystal, and bubble-jetting member and protein-adsorbing-bubble-jetting member used in said device. A bubble-jetting member is used in a protein crystal device to jet bubbles into a protein solution to thereby allow protein crystals to be obtained, the bubble-jetting member comprising: a core formed of a conductive material; a shell part formed of an insulating material, including an extended section extending from the tip of the core, and in which at least a portion closely adheres to the core to cover the core; and a gap having a bubble-jetting port, the gap being formed between the extended section and the tip of the core.
    Type: Grant
    Filed: August 27, 2014
    Date of Patent: April 7, 2020
    Assignee: JAPAN SCIENCE AND TECHNOLOGY AGENCY
    Inventors: Yoko Yamanishi, Fumihito Arai, Hiroki Kuriki
  • Patent number: 10611135
    Abstract: Embodiments of the present invention relate to a device comprising a platform comprising a layer of a 2-dimensional material. The device further comprises a plurality of electrodes and one or more molecules arranged on the platform. The device is configured to apply control signals to the plurality of electrodes to position the molecules by means of an electric field. Embodiments of the invention further concern a corresponding method for fabricating such a device and a method for positioning molecules by such a device.
    Type: Grant
    Filed: March 15, 2017
    Date of Patent: April 7, 2020
    Assignee: INTERNATIONAL BUSINESS MACHINES CORPORATION
    Inventor: Pio Peter N. Nirmalraj
  • Patent number: 10571475
    Abstract: Methods and systems for rapid and efficient screening of monoclonal antibodies and antibody-secreting cells, and particularly of single antibody-secreting cells, for both primary and functional characteristics, and particularly cell to cell interactions, in a microfluidic system, in particular an inverted open microwell system, where the particle(s) is not bound to a substrate.
    Type: Grant
    Filed: December 5, 2011
    Date of Patent: February 25, 2020
    Assignee: Cellply S.R.L.
    Inventors: Massimo Bocchi, Roberto Guerrieri
  • Patent number: 10564125
    Abstract: A method of forming a semiconductor structure includes forming a substrate, forming an anchor layer, and forming one or more self-aligned nanotip pillar pairs disposed vertically between the substrate and the anchor layer. A given one of the nanotip pillar pairs comprises a bottom nanotip pillar and a top nanotip pillar, the bottom nanotip pillar comprising a base portion disposed on a top surface of the substrate and the top nanotip pillar comprising a base portion disposed in the anchor layer. The bottom nanotip pillar and the top nanotip pillar comprise sidewalls that taper to points as distance from the respective base portions increases.
    Type: Grant
    Filed: December 14, 2017
    Date of Patent: February 18, 2020
    Assignee: International Business Machines Corporation
    Inventors: Juntao Li, Kangguo Cheng, Peng Xu, Heng Wu
  • Patent number: 10557150
    Abstract: In an illustrative embodiment, automated instruments comprising one or more flow-through electroporation devices or modules are provided to automate transformation of nucleic acids in live cells.
    Type: Grant
    Filed: September 14, 2019
    Date of Patent: February 11, 2020
    Assignee: Inscripta, Inc.
    Inventors: Jorge Bernate, Don Masquelier, Phillip Belgrader
  • Patent number: 10556234
    Abstract: An integrated system to isolate and diagnose circulating tumor cells (CTCs) within a cellular sample includes an isolating mechanism to isolate and trap large biological cells at a detection zone from among the cellular sample based on cells size, and includes a diagnosing mechanism to diagnose CTCs among the trapped large biological cells, based on cells electrical impedance.
    Type: Grant
    Filed: August 16, 2016
    Date of Patent: February 11, 2020
    Inventors: Mohammad Abdolahad, Seiedali Hosseini, Somayeh Zanganeh
  • Patent number: 10519037
    Abstract: An intermediate transfer surface includes a substrate, a two-dimensional array of electrodes, a dielectric spacer layer on the two-dimensional array of electrodes, and a voltage controller electrically connected to the array of electrodes. A method of manufacturing an intermediate transfer surface, depositing an array of etch stops on a conductive surface, etching the conductive surface to form mesas of the conductive surface separated by gaps, and coating the mesas with a dielectric coating. A microassembly system includes an assembly surface having a first two dimensional array of potential wells on a first surface, a first voltage source electrically connected to the first array of potential wells, an intermediate transfer surface having a second two dimensional array of potential wells on a second surface arranged to face the first surface, and a second voltage source electrically connected to the second array of potential wells.
    Type: Grant
    Filed: January 18, 2016
    Date of Patent: December 31, 2019
    Assignee: PALO ALTO RESEARCH CENTER INCORPORATED
    Inventors: Sourobh Raychaudhuri, Jeng Ping Lu, David K. Biegelsen
  • Patent number: 10495580
    Abstract: An inspection device (1) inspects an amount of dielectric particles contained in a sample liquid. The inspection device includes a dielectric collection unit (3), a pump unit (10) and an AC voltage supply unit (11). The dielectric collection unit includes at least one pair of electrodes (41, 42) and a flow channel (13) extending in a predetermined direction on the pair of electrodes. The pump unit is configured to feed the sample liquid to follow the flow channel in the predetermined direction. The AC voltage supply unit is configured to supply, to the pair of electrodes, an AC voltage with a predetermined frequency to cause dielectrophoresis for dielectric particles in the fed sample liquid. The dielectric collection unit includes a plurality of slit regions (Rs) aligned in the predetermined direction between the pair of electrodes. Each of the plurality of slit regions is separated from each other within the flow channel.
    Type: Grant
    Filed: October 5, 2016
    Date of Patent: December 3, 2019
    Assignee: AFI Corporation
    Inventors: Takaharu Enjoji, Yoshikazu Wakizaka, Satoshi Uchida, Eiko Kato, Masayo Takano
  • Patent number: 10478822
    Abstract: The present disclosure provides a reagent cartridge configured for use in an automated multi-module cell processing environment.
    Type: Grant
    Filed: July 29, 2019
    Date of Patent: November 19, 2019
    Assignee: Inscripta, Inc.
    Inventors: Don Masquelier, Phillip Belgrader, Brian Van Hatten, Jorge Bernate, Bruce Chabansky
  • Patent number: 10413912
    Abstract: Disclosed are apparatuses, systems, and methods for programmable fluidic processors. In one embodiment, the invention involves manipulating droplets across a reaction surface of the processor substantially contact-free of any surfaces. The reaction surface and the electrodes of the processor may include a coating repelling the droplets. Further, the present invention provides for a suitable suspending medium for repelling droplets away from fixed surfaces.
    Type: Grant
    Filed: March 2, 2015
    Date of Patent: September 17, 2019
    Assignee: THE BOARD OF REGENTS OF THE UNIVERSITY OF TEXAS SYSTEM
    Inventors: Peter R. C. Gascoyne, Jody Vykoukal, Jon Schwartz
  • Patent number: 10406525
    Abstract: The present disclosure provides a reagent cartridge configured for use in an automated multi-module cell processing environment.
    Type: Grant
    Filed: June 25, 2019
    Date of Patent: September 10, 2019
    Assignee: Inscripta, Inc.
    Inventors: Don Masquelier, Phillip Belgrader, Brian Van Hatten, Jorge Bernate, Bruce Chabansky
  • Patent number: 10384204
    Abstract: A system for operating an electrokinetic device includes a support configured to hold and operatively couple with the electrokinetic device, an integrated electrical signal generation subsystem configured to apply a biasing voltage across a pair of electrodes in the electrokinetic device, and a light modulating subsystem configured to emit structured light onto the electrokinetic device. The system can further include a thermally controlled flow controller, and/or be configured to measure impedance across the electrokinetic device. The system can be a light microscope, including an optical train. The system can further include a light pipe, which can be part of the light modulating subsystem, and which can be configured to supply light of substantially uniform intensity to the light modulating subsystem or directly to the optical train.
    Type: Grant
    Filed: December 9, 2015
    Date of Patent: August 20, 2019
    Assignee: Berkeley Lights, Inc.
    Inventors: Andrew W. McFarland, Daniele Malleo, J. Tanner Nevill, Russell A. Newstrom, Keith J. Breinlinger, Paul M. Lundquist, Justin K. Valley, Jonathan Cloud Dragon Hubbard
  • Patent number: 10376889
    Abstract: The present disclosure provides a reagent cartridge configured for use in an automated multi-module cell processing environment.
    Type: Grant
    Filed: April 10, 2019
    Date of Patent: August 13, 2019
    Assignee: Inscripta, Inc.
    Inventors: Don Masquelier, Phillip Belgrader, Brian Van Hatten, Jorge Bernate, Bruce Chabansky
  • Patent number: 10369565
    Abstract: Example methods, apparatus, systems for diluting samples are disclosed. An example method includes depositing a first fluid droplet on a first electrode of a plurality of electrodes. The first electrode has a first area. The first fluid droplet has a first volume associated with the first area. The example method includes depositing a second fluid droplet on a second electrode of the plurality of electrodes. The second electrode has a second area. The second fluid droplet has a second volume associated with the second area. The second volume is different than the first volume. The example method includes forming a combined droplet by selectively activating at least one of the first electrode or the second electrode to cause one of the first fluid droplet or the second fluid droplet to merge with the other of the first fluid droplet or the second fluid droplet.
    Type: Grant
    Filed: December 21, 2015
    Date of Patent: August 6, 2019
    Assignee: Abbott Laboratories
    Inventor: Andrew Fischer
  • Patent number: 10316363
    Abstract: Provided is a sensing apparatus comprising a chip for integrated amplification and sequencing of a template polynucleotide in a sample. The apparatus comprises a chip with at least one ISFET in a well or chamber, amplification means for amplifying the template polynucleotide on a surface of said chip and comprising at least one heating means suitable for conducting amplification of the template polynucleotide at temperatures elevated with respect to room temperature, and sequencing means for sequencing the amplified template polynucleotide in said well or chamber. Methods of use are also provided.
    Type: Grant
    Filed: April 24, 2017
    Date of Patent: June 11, 2019
    Assignee: DNAE Group Holdings Limited
    Inventors: Zahid Ansari, Krishna Amin, Ginny Jorgensen, Kurt Kolb, Daniel Morley, Alpesh Patel, Samuel Reed, Leila Shepherd, Christofer Toumazou
  • Patent number: 10307769
    Abstract: There is described herein methods and devices for confining and/or manipulating molecules. At least one molecule is introduced into a fluidic chamber. The fluidic chamber is formed inside a device comprising at least one first electrode having a first surface spaced from at least one second electrode having a second surface facing the first surface. The at least one second electrode has a plurality of dielectric structures arranged to form openings along the second surface. At least one electrical signal is applied across the at least one first electrode and the at least one second electrode to generate a non-uniform electric field having electric field lines extending from the first surface of the at least one first electrode to the second surface of the at least one second electrode in the openings formed between the dielectric structures. The at least one electrical signal has a frequency level causing the at least one molecule to move inside the fluidic chamber in accordance with a predetermined movement.
    Type: Grant
    Filed: May 16, 2017
    Date of Patent: June 4, 2019
    Assignee: THE ROYAL INSTITUTION FOR THE ADVANCEMENT OF LEARNING/MCGILL UNIVERSITY
    Inventors: Sara Mahshid, Mohammed Jalal Ahamed, Walter Reisner, Robert Sladek
  • Patent number: 10252269
    Abstract: Methods and devices are provided for focusing and/or sorting activated T cells. The device comprises a microfluidic channel comprising a plurality of electrodes arranged to provide dielectrophoretic (DEP) forces that are perpendicular to forces from hydrodynamic flows along the channel. The device may be configured to apply voltages to a plurality of electrodes in a first upper region of the microfluidic channel to focus the cells into a single flow, and to apply different voltages to a plurality of electrodes in a second downstream region of the microfluidic channel to sort cells based on size. The output of the microfluidic channel may diverge into multiple channels, wherein cells of various sorted sizes are directed into the appropriate output channel.
    Type: Grant
    Filed: November 17, 2017
    Date of Patent: April 9, 2019
    Assignees: NANTBIO, INC., NANOCAV, LLC
    Inventors: Yu-Chun Kung, Ting-Hsiang Wu, Peter Sieling, Kayvan Niazi
  • Patent number: 10208095
    Abstract: Non-centrifugal methods for generating a solution rich in interleukin-1 receptor antagonist from a tissue comprising cytokine-producing cells. The solution rich in IL-1ra can also include at least one of sTNF-RI, sTNF-RII, IGF-I, EGF, HGF, PDGF-AB, PDGF-BB, VEGF, TGF-?1, and sIL-1 RII.
    Type: Grant
    Filed: March 15, 2013
    Date of Patent: February 19, 2019
    Assignee: Biomet Manufacturing, LLC
    Inventors: Michael D. Leach, Jennifer E. Woodell-May, Joel C. Higgins, Krista O'Shaughnessey
  • Patent number: 10100418
    Abstract: According to one embodiment, an oxidation electrode includes: a collector; an oxidation catalyst formed on the collector; and a modified organic molecule which is bonded to the surface of the oxidation catalyst, and comprises a cationic functional group.
    Type: Grant
    Filed: September 9, 2016
    Date of Patent: October 16, 2018
    Assignee: KABUSHIKI KAISHA TOSHIBA
    Inventors: Jun Tamura, Satoshi Mikoshiba, Akihiko Ono, Yuki Kudo, Ryota Kitagawa, Chingchun Huang
  • Patent number: 10081015
    Abstract: A device for trapping at least one microparticle in a fluid flow is suggested. The device comprises a trapping element and an electrode. The trapping element is configured for trapping the at least one microparticle and has at least one recess for receiving the at least one microparticle. The electrode is configured for generating an asymmetric electric field. In operation, at least one microparticle of a plurality of microparticles passing through the asymmetric electric field is forced into the at least one recess of the trapping element.
    Type: Grant
    Filed: July 12, 2015
    Date of Patent: September 25, 2018
    Assignee: International Business Machines Corporation
    Inventors: Jaione Tirapu Azpiroz, Emmanuel Delamarche, Claudius Feger, Yuksel Temiz
  • Patent number: 10001449
    Abstract: Systems, methods, and test kits for detecting and quantifying an analyte level in a biological fluid sample using impedance measurements, are disclosed. The fluid sample is applied to a lateral flow strip, and impedance of the strip is measured as the assay dries. Analysis of the drying-dependent impedance measurements indicates the presence and quantity of the analyte in the fluid sample.
    Type: Grant
    Filed: December 15, 2014
    Date of Patent: June 19, 2018
    Assignee: Church & Dwight Co., Inc.
    Inventors: Albert Nazareth, Shang Li, Timothy Snowden, Giles H. W. Sanders, Anthony Cass
  • Patent number: 9981273
    Abstract: The procedure of dielectric electrophoresis (dielectrophoresis or DEP) utilizes field-polarized particles that move under the application of positive (attractive) and/or negative (repulsive) applied forces. This invention uses negative dielectric electrophoresis (negative dielectrophoresis or nDEP) within a microchannel separation apparatus to make particles move (detached) or remain stationary (attached). In an embodiment of the present invention, the nDEP force generated was strong enough to detach Ag-Ab (antigen-antibody) bonds, which are in the order of 400 pN (piconewtons) while maintaining the integrity of the system components.
    Type: Grant
    Filed: September 30, 2013
    Date of Patent: May 29, 2018
    Assignee: The Board of Trustees of the Leland Stanford Junior University
    Inventors: Mehdi Javanmard, Sam Emaminejad, Janine Mok, Michael N. Mindrinos
  • Patent number: 9983169
    Abstract: An integrated fluidic circuit has a supporting surface that carries a first fluid to be moved at a first functional region; a dielectric structure, defining the supporting surface; and an electrode structure, coupled to the dielectric structure for generating an electric field at the first functional region, such as to modify electrowetting properties of the interface between the first fluid and the supporting surface. The dielectric structure has a first spatially variable dielectric profile at the first functional region, thus determining a corresponding spatially variable profile of the electric field, and, consequently, of the electrowetting properties of the interface between the first fluid and the supporting surface. The integrated fluidic circuit may achieve mixing between the first fluid and a second fluid.
    Type: Grant
    Filed: September 17, 2014
    Date of Patent: May 29, 2018
    Assignee: STMICROELECTRONICS S.R.L.
    Inventor: Alessandro Paolo Bramanti