Electrophoresis Or Electro-osmosis Processes And Electrolyte Compositions Therefor When Not Provided For Elsewhere Patents (Class 204/450)
  • Patent number: 11813234
    Abstract: Method of treating hyperglycemia induced Red Blood Cell Disease/Dysfunction (RBCD) caused by generation of early and late glycation end products. Method of treating hemolytic anemia, smoldering hemolytic anemia, sickle cell anemia, hemorrhagic diseases, hemorrhagic stroke, hemorrhagic bleeding. Method of treating RBCD to prevent progression to diabetes associated vascular complications referred to as Syndrome X, particularly to prevent progression to chronic kidney disease, coronary vascular disease, and peripheral vascular disease.
    Type: Grant
    Filed: August 22, 2016
    Date of Patent: November 14, 2023
    Inventors: Paiboon Maraprygsavan, Friedrich-Wilhelm Kuehne
  • Patent number: 11747701
    Abstract: A capsule comprising a capsule wall and an electrophoretic fluid encapsulated by the capsule wall. The capsule wall comprises a cross-linked nonionic, water-soluble or water-dispersible polymer. The electrophoretic fluid comprises a suspending fluid, first pigment particles, second pigment particles, and third pigment particles. In some embodiments, the electrophoretic fluid includes a fourth electrophoretic particle. The first, second, and third particles are electrically charged, suspended in the suspending fluid, and capable of moving through the suspending fluid upon application of an electric field to the capsule.
    Type: Grant
    Filed: December 22, 2020
    Date of Patent: September 5, 2023
    Assignee: E Ink Corporation
    Inventors: Stephen J. Telfer, Rosa Casado, Sergey Egorov, Erin Criswell Chang, Jay William Anseth, Ana L. Lattes, Lynne A. McCullough
  • Patent number: 11745189
    Abstract: A nanocarbon separation device includes a separation tank that is configured to accommodate a dispersion liquid including nanocarbons, a first electrode provided at an upper part in the separation tank, a second electrode provided at a lower part in the separation tank, an evaluation unit that is configured to evaluate a physical state or a chemical state of the dispersion liquid, and a determination unit that is configured to determine a separation state between metallic nanocarbons and semiconducting nanocarbons included in the dispersion liquid from the physical state or the chemical state.
    Type: Grant
    Filed: October 26, 2017
    Date of Patent: September 5, 2023
    Inventor: Kazuki Ihara
  • Patent number: 11740255
    Abstract: A system includes a droplet actuator having a droplet-operation gap between top and bottom substrates, a reservoir(s) external to and coupled to the droplet actuator, the reservoir(s) sized for a large-volume fluid, and pressure source(s) external to the droplet actuator and coupled to the at least one reservoir. Operation of the system includes filling the reservoir(s) with a large volume of fluid(s), dispensing droplet(s) of the fluid(s) to the droplet-operation gap using the pressure source(s) as part of performing a droplet operation(s). Movement of the droplet(s) may be effectuated by activating the droplet actuator.
    Type: Grant
    Filed: November 9, 2018
    Date of Patent: August 29, 2023
    Assignee: ILLUMINA, INC.
    Inventors: Jennifer Olivia Foley, Darren Segale, Cyril Delattre
  • Patent number: 11731131
    Abstract: The present disclosure discloses a method for driving a microfluidic chip including: controlling a first electrode that currently carries a droplet to be electrically connected to a first power supply by a first switch circuit connected to the first electrode; after controlling the first electrode to be in electrical connection to the first power supply for a first period of time, controlling the first electrode to be in electrical connection to a second power supply for a second period of time; and after the second period of time, continuing to control the first electrode to keep disconnected from two power supplies.
    Type: Grant
    Filed: December 23, 2019
    Date of Patent: August 22, 2023
    Inventor: Chengqian Li
  • Patent number: 11703616
    Abstract: An example device includes a fluid lens, where the fluid lens includes a membrane, a substrate, and a fluid located within an enclosure formed at least in part by the membrane and the substrate. The membrane may be an elastic membrane. The fluid may include an amount of an additive that is effective to appreciably reduce bubble formation within the fluid, such as when a negative pressure is applied to the fluid. In some examples, the additive may include particles, such as nanoparticles. The additive may include a thixotropic agent that helps to impart an appreciable thixotropic property to the fluid. Various other methods, systems, and computer-readable media are also disclosed.
    Type: Grant
    Filed: October 20, 2020
    Date of Patent: July 18, 2023
    Assignee: Meta Platforms Technologies, LLC
    Inventors: Hayden Erik Hernández, Thomas Norman Llyn Jacoby, Robert Edward Stevens
  • Patent number: 11619020
    Abstract: Disclosed is a sludge three-dimensional electroosmosis drainage reinforcing method based on a novel electric geotextile complex, and belongs to the field of soft soil foundation reinforcement. A novel electric geotextile complex is adopted, and a designed multidirectional three-dimensional continuous electroosmosis drainage method is adopted to perform rapid drainage reinforcement treatment on sludge, wherein the electric geotextile complex is prepared by combining fibers and conductive materials with a flexible drainage plate and has the effects of electric conductive, drainage, corrosion resistance and reinforcement. The proposed drainage method can realize the electroosmotic drainage in vertical and horizontal directions. Through layer by layer electroosmosis from bottom to top, the consolidation drainage effect of sludge in the lower layer can be enhanced by the increasing loading pressure from the upper sludge.
    Type: Grant
    Filed: December 9, 2021
    Date of Patent: April 4, 2023
    Inventors: Xinyu Xie, Shangqi Ge, Lingwei Zheng, Xunli Zhang, Huai Nie, Xuexin Huang, Kang Zhang, Puxiu Dai, Minyang Sun, Chunyang Liu
  • Patent number: 11598699
    Abstract: Among other things, a diluted sample is generated based on mixing a small sample of blood with a one or more diluents. A thin film of the diluted sample is formed on the surface of a contact optical microscopy sensor. Red blood cells within a portion of the thin film of the diluted sample are illuminated using light of a predetermined wavelength. One or more images of the diluted sample are acquired based on illuminating the red blood cells within the portion of the thin film of the diluted sample. The acquired one or more images of the diluted sample are then processed. The mean corpuscular hemoglobin in the red blood cells within the portion of the thin film of the diluted sample is determined based on processing the acquired images of the diluted sample.
    Type: Grant
    Filed: August 14, 2020
    Date of Patent: March 7, 2023
    Assignee: Alentic Microscience Inc.
    Inventors: Alan Marc Fine, Hershel Macaulay
  • Patent number: 11596901
    Abstract: A technology that provides various modular biomimetic microfluidic modules emulating varieties of microvasculature in body. These microfluidic-base capillaries and lymphatic Technology modules are constructed as multilayered-microfluidic microchannels of various shapes, and aspect ratios using diverse biocompatible microfluidic polymers. Then, various semipermeable membranes are sandwiched in between these multilayered microfluidic microchannels. These membranes have different chemical, physical characteristics and MWCO values. Consequently, this design will produce much smaller dimension channels similar to human vasculature to achieve biomimetic properties like of human organs and tissues. By interchanging microfluidic-layers or the membranes various diverse modules are designed that act as building blocks for constructing various medical devices, various forms of dialysis devices including albumin and lipid dialysis, water purification, bioreactors, bio-artificial organ support systems.
    Type: Grant
    Filed: May 29, 2020
    Date of Patent: March 7, 2023
    Inventor: Saeid Mordechai Nosrati
  • Patent number: 11567085
    Abstract: The present invention provides photo-cleavable anionic surfactants, particularly 4-hexylphenylazosulfonate (Azo) and sodium 4-hexylphenylazosulfonate derivatives, which can be rapidly degraded upon UV irradiation, for top-down and bottom-up proteomics. These surfactants can effectively solubilize proteins and peptide fragments with performance comparable to sodium dodecyl sulfate (SDS) and are compatible with mass spectrometry analysis of the solubilized proteins and peptide fragments. Top-down proteomic studies using the present photo-cleavable anionic surfactants has allowed the detection of 100-fold more unique proteoforms as compared to controls and has enabled the solubilization of membrane proteins for comprehensive characterization of protein post-translational modifications.
    Type: Grant
    Filed: June 4, 2019
    Date of Patent: January 31, 2023
    Assignee: Wisconsin Alumni Research Foundation
    Inventors: Ying Ge, Song Jin, Tania Guardado Alvarez, Kyle Brown
  • Patent number: 11560635
    Abstract: Disclosed are an electrode for gas generation, a method of preparing the electrode, and a device including the electrode for gas generation. The electrode includes a gas generating electrode layer and a three-dimensional (3D) super-aerophobic layer formed on at least one portion of the gas generating electrode layer and including porous hydrogel.
    Type: Grant
    Filed: September 23, 2019
    Date of Patent: January 24, 2023
    Inventors: Jungki Ryu, Dong Woog Lee, Dasom Jeon, Jinwoo Park
  • Patent number: 11534762
    Abstract: A microfluidic device, a method of using a microfluidic device and a micro total analysis system are provided. The microfluidic device includes a first substrate, and the first substrate includes a base substrate and a pixel array. The pixel array includes a plurality of pixels and is on the base substrate, and each of the plurality of pixels includes a driving electrode. Driving electrodes of two adjacent pixels are in different layers.
    Type: Grant
    Filed: May 23, 2019
    Date of Patent: December 27, 2022
    Inventors: Yingming Liu, Haisheng Wang, Xiaochuan Chen, Xue Dong, Xiaoliang Ding, Pinchao Gu, Lei Wang, Changfeng Li, Ping Zhang
  • Patent number: 11530445
    Abstract: The present invention provides methods, compositions, and systems for distributing molecules and complexes into reaction sites. In particular, the methods, compositions, and systems of the present invention result in an active loading of molecules and complexes into reaction sites with improved efficiency over loading by passive diffusion methods alone.
    Type: Grant
    Filed: June 9, 2020
    Date of Patent: December 20, 2022
    Assignee: Pacific Biosciences of California, Inc.
    Inventor: Stephen Turner
  • Patent number: 11465147
    Abstract: A method and device for transfecting a cell to introduce an exogenous material into the cell. The method includes exposing the cell to a region of unsteady flow in the presence of an electric field to encourage introduction of the exogenous material into a cell without lysing the cell.
    Type: Grant
    Filed: June 29, 2019
    Date of Patent: October 11, 2022
    Assignee: INDEE. PTY. LTD.
    Inventors: Ryan Pawell, Amy Twite, Geoff Facer, Katherine Lau, Adrian Lievano, Julyana Acevedo
  • Patent number: 11459614
    Abstract: Disclosed herein is a method of performing polymerase chain reaction (PCR) to determine a repeating number of CGG sequence in fragile X mental retardation 1 (FMR1) gene. Also disclosed herein are a kit, and uses thereof in making a diagnosis of Fragile X syndrome (FXS) in a human subject based on the repeating number of the CGG sequence in a DNA sample isolated from the human subject. According to embodiments of the present disclosure, the kit comprises four primers, in which the first primer comprises a first polynucleotide sequence of SEQ ID NO: 1; the second primer comprises a second polynucleotide sequence of SEQ ID NO: 2; the third primer comprises a third polynucleotide sequence of SEQ ID NO: 3, and a non-human sequence disposed at and connected to the 5?-end of the third polynucleotide sequence; and the fourth primer comprises the non-human sequence.
    Type: Grant
    Filed: January 31, 2020
    Date of Patent: October 4, 2022
    Inventors: Yi-Yi Kuo, Yu-Chiao Hsiao, I-Fan Chiu, Lai-Ha Chung, Shu-Ju Lee
  • Patent number: 11422504
    Abstract: The invention provides a method, apparatus and system for separating blood and other types of cellular components, and can be combined with holographic optical trapping manipulation or other forms of optical tweezing. One of the exemplary methods includes providing a first flow having a plurality of blood components; providing a second flow; contacting the first flow with the second flow to provide a first separation region; and differentially sedimenting a first blood cellular component of the plurality of blood components into the second flow while concurrently maintaining a second blood cellular component of the plurality of blood components in the first flow. The second flow having the first blood cellular component is then differentially removed from the first flow having the second blood cellular component. Holographic optical traps may also be utilized in conjunction with the various flows to move selected components from one flow to another, as part of or in addition to a separation stage.
    Type: Grant
    Filed: August 27, 2021
    Date of Patent: August 23, 2022
    Assignee: ABS GLOBAL, INC.
    Inventors: Daniel Mueth, Joseph Plewa, Jessica Shireman, Amy Anderson, Lewis Gruber, Neil Rosenbaum
  • Patent number: 11408849
    Abstract: The presence of oxygen or red blood cells in a sample applied to an electrochemical test strip that makes use of a reduced mediator is corrected for by an additive correction factor that is determined as a function of the temperature of the sample and a measurement that reflects the oxygen carrying capacity of the sample. The measured oxygen carrying capacity can also be used to determine hematocrit and to distinguish between blood samples and control solutions applied to a test strip.
    Type: Grant
    Filed: April 6, 2020
    Date of Patent: August 9, 2022
    Assignee: AgaMatrix, Inc.
    Inventors: Ian Harding, Richard Williams, Sridhar Iyengar
  • Patent number: 11366943
    Abstract: A computer-implemented method, computer program product and prototyping platform creates a design blueprint for a substrate-based microfluidic device. A design and prototyping platform receives at least one blueprint parameter and at least one constraint associated with a proposed substrate-based microfluidic device including a hydrophilic material and arrangement of a pattern of a hydrophobic material. The platform determines an arrangement of a plurality of microfluidic device elements as candidates for implementation of the proposed substrate-based microfluidic device and outputs a design blueprint of the proposed substrate-based microfluidic device.
    Type: Grant
    Filed: June 18, 2019
    Date of Patent: June 21, 2022
    Inventors: Jaione Tirapu Azpiroz, Ademir Ferreira da Silva, Matheus Esteves Ferreira, Ricardo Luis Ohta, Mathias B. Steiner
  • Patent number: 11351256
    Abstract: Stable lyophilized therapeutic protein compositions and their methods of manufacture are provided. Specifically, the use of water as a solid cake plasticizer and protein stabilizer is described. Also, the inclusion of a multicomponent stabilizer comprising a larger molecular entity and a smaller molecular entity is described. Also, the inclusion of post-drying annealing under certain conditions improves protein stability. Proteins are predicted to remain stable over 24 months at 25° C.
    Type: Grant
    Filed: October 6, 2017
    Date of Patent: June 7, 2022
    Assignee: Regeneron Pharmaceuticals, Inc.
    Inventors: Xiaolin Tang, David Brett Ludwig
  • Patent number: 11342153
    Abstract: Provided is a field emission device including a cathode electrode and an anode electrode, which are spaced apart from each other, an emitter disposed on the cathode electrode, a gate electrode disposed between the cathode electrode and the anode electrode and including a gate opening that overlaps the emitter, and a plurality of alignment electrodes disposed between the gate electrode and the cathode electrode. Here, the alignment electrodes surround a side surface of the emitter.
    Type: Grant
    Filed: March 15, 2021
    Date of Patent: May 24, 2022
    Inventors: Jae-Woo Kim, Yoon-Ho Song, Eun Sol Ko
  • Patent number: 11325124
    Abstract: An apparatus for separating an analyte from a test sample, such as bacteria from blood components, based on their dielectric properties, localizing or condensing the analyte, flushing substantially all remaining waste products from the test sample, and detecting low concentrations of the analyte. The module array includes a plurality of microfluidic channels with connecting microfluidic waste channels for directing undesired material away from the analyte. An electric field is applied causing a positive dielectrophoretic force to the analyte to capture the analyte. The electric field is applied to at least one electrode having a plurality of concentric rings or concentric arcs extending radially outwards from a center point, electrically connected to a voltage source such that when voltage is applied to the at least one electrode, the concentric rings or concentric arcs alternate in voltage potential.
    Type: Grant
    Filed: December 27, 2019
    Date of Patent: May 10, 2022
    Assignee: Fluid-Screen, Inc.
    Inventors: Monika Weber, Siu Lung Lo, Hazael Fabrizio Montanaro Ochoa, Christopher Daniel Yerino, Mark A. Reed
  • Patent number: 11291991
    Abstract: A sample carrier device including a single substrate, a penetration structure and a fixing structure is provided. The penetration structure is formed on a side of the substrate. The penetration structure has a fluid passage. The fixing structure is formed on a side of the penetration structure. The sample carrier device is divided into an end portion, an observation portion and an operation portion. The user can separate the observation portion from the end portion by operating the operation portion. After the observation portion is separated from the end portion, the user can inject the sample into the fluid passage through a port of the fluid passage exposed to the observation portion. Once the sample is carried by the fluid passage of the observation portion, the user can seal the port of the fluid passage and place the observation portion in an electron microscope device.
    Type: Grant
    Filed: September 20, 2019
    Date of Patent: April 5, 2022
    Assignee: Materials Analysis Technology Inc.
    Inventor: Hung-Jen Chen
  • Patent number: 11262333
    Abstract: A method for concentrating electrically charged objects in a non-Newtonian liquid medium comprises: feeding a sample containing electrically charged objects into a channel having a flow axis, a first transverse cross-section orthogonal to the flow axis, and at least one second transverse cross-section orthogonal to the flow axis, one dimension of the second cross-section being less than the corresponding dimension of the first cross-section; and applying a hydrodynamic flow in a direction of the channel together with the application, in the opposite direction, of an electric field in the channel, thus making it possible to move the electrically charged objects in the channel along the flow axis from the first cross-section to the second cross-section, stop the objects, and concentrate the objects in at least one area upstream from the second transverse cross-section.
    Type: Grant
    Filed: August 3, 2015
    Date of Patent: March 1, 2022
    Inventors: Aurélien Bancaud, Hubert Ranchon, Thierry Leichle, Pattamon Teerapanich
  • Patent number: 11255769
    Abstract: A microfluidic device includes: a microchannel defining a flow path; a Brownian motor structure comprising two or more sorting channels having distinct ratchet topographies, the Brownian motor structure in fluid communication with the microchannel; and a filter extending transversely to the microchannel, the filter configured to filter particles, subject to sizes thereof, in a liquid advancing along the flow path, whereby smaller particles of the liquid can pass downstream of the filter in the flow path, and larger particles of the liquid are directed to the Brownian motor structure to be sorted out according to sizes thereof via the sorting channels.
    Type: Grant
    Filed: August 1, 2019
    Date of Patent: February 22, 2022
    Assignee: International Business Machines Corporation
    Inventors: Armin Knoll, Francesca Ruggeri, Christian Michael Schwemmer
  • Patent number: 11251791
    Abstract: Disclosed are a microfluid detection device, system and method, a processing device and a storage medium, in the field of biochemistry. The device includes a first substrate and a second substrate facing each other, and a microfluid chamber between the first substrate and the second substrate; wherein the first substrate has a plurality of photoelectric sensors and an output circuit, each of the photoelectric sensors is configured to convert an optical signal passing through the second substrate and the microfluid chamber to an electrical signal, and the output circuit is configured to output the electrical signal obtained by the photoelectric sensor.
    Type: Grant
    Filed: June 28, 2019
    Date of Patent: February 15, 2022
    Assignee: BOE Technology Group Co., Ltd.
    Inventors: Yuzhen Guo, Haisheng Wang, Yingming Liu, Xiaoliang Ding, Yanling Han, Xueyou Cao
  • Patent number: 11235286
    Abstract: The present disclosure describes compositions and methods for preparing membrane protein nanosheets and two-dimensional crystals. In particular, the methods employ a solvent. A mixture of a polymer and a membrane protein is solubilized in the solvent, applied to a substrate, and subsequently dried to form the nanosheet or two-dimensional crystal. Applicants have surprisingly found that the membrane proteins maintain their structure when exposed to solvents during the short processing time utilized.
    Type: Grant
    Filed: May 16, 2019
    Date of Patent: February 1, 2022
    Assignee: The Penn State Research Foundation
    Inventors: Manish Kumar, Tingwei Ren, Woochul Song, Yu-Ming Tu
  • Patent number: 11235325
    Abstract: A microfluidic control system for controlling an EWOD device has an enhanced thermal control system for generating a temperature profile within an EWOD device that is inserted into the microfluidic control system. The microfluidic control system includes a housing that defines an aperture for receiving an EWOD device; an active heating component located within the housing at a base of the aperture; and a lid attached to the housing that is moveable between a closed position and an open position, the lid including a thermal control component. When the lid is in the closed position, the thermal control component is positioned at the aperture and aligned oppositely from the active heating component. The active heating component may include a plurality of independently controllable individual heating elements, and the thermal control component may include a respective plurality of individual thermal control elements.
    Type: Grant
    Filed: November 11, 2019
    Date of Patent: February 1, 2022
    Assignee: Sharp Life Science (EU) Limited
    Inventors: Philip Mark Shryane Roberts, Adam Christopher Nightingale
  • Patent number: 11179721
    Abstract: Provided herein are microfluidic devices that can be configured to generate an electrophoretic flow that is in opposition to a fluid flow through a microcapillary of a microfluidic device provided herein. Also provided herein are methods that include adding an amount of particle to the inlet area of a microfluidic device as provided herein, generating a first fluid flow through a microcapillary of a microfluidic device provided herein; and applying a uniform electric field to the microfluidic device, where the uniform electric field generates an electrophoretic flow that is in opposition to the fluid flow.
    Type: Grant
    Filed: July 21, 2016
    Date of Patent: November 23, 2021
    Assignee: University of Florida Research Foundation, Inc.
    Inventors: Jason E. Butler, Anthony J. Ladd, Mert Arca
  • Patent number: 11168847
    Abstract: The invention relates to a directional lighting device (10) which comprises: a light emission source (30); a cover (40) covering the light emission source (30), and provided with an inner wall (41) and an outer wall (42) which delimit an inter-wall space (44), and filled with a fluid, the cover (40) comprising transmission zones (45), each formed of an inner zone (45a) and an outer zone (45b), facing one another, and at which an electric field is capable of being applied to the functional fluid by means of a first electrode (46a) and a second electrode (46b), the functional fluid being adapted to, under the effect of an electric field sensed at a given transmission zone, form with the latter a window transparent to the luminous radiation, and be either opaque or reflective and/or diffusive to said radiation in the remainder of the inter-wall volume (43).
    Type: Grant
    Filed: February 1, 2021
    Date of Patent: November 9, 2021
    Inventors: Marianne Consonni, Anis Daami, Frédéric Sermet
  • Patent number: 11142740
    Abstract: The present disclosure provides automated modules and instruments for improved detection of nuclease genome editing of live cells. The disclosure provides improved modules—including high throughput modules—for screening cells that have been subjected to editing and identifying and selecting cells that have been properly edited.
    Type: Grant
    Filed: August 8, 2019
    Date of Patent: October 12, 2021
    Assignee: Inscripta, Inc.
    Inventors: Andrew Garst, Michael Graige, Richard Fox, Eileen Spindler, Amy Hiddessen, Phillip Belgrader, Don Masquelier, Bruce Chabansky
  • Patent number: 11136285
    Abstract: The present invention relates to a process for the continuous production of nitrobenzene by the nitration of benzene with nitric acid and sulphuric acid under adiabatic conditions, not the entire production plant being shut down during a production stop, but the production plant being entirely or at least partly operated in recirculation mode. The invention further relates to a plant for producing nitrobenzene and to a method for operating a plant for producing nitrobenzene.
    Type: Grant
    Filed: June 15, 2018
    Date of Patent: October 5, 2021
    Assignee: Covestro Deutschland AG
    Inventors: Thomas Knauf, Peter Drinda
  • Patent number: 11119068
    Abstract: The present invention provides, in some embodiments, an isotachophoresis (ITP) apparatus, a kit comprising same and method of use thereof for the focusing analytes of interest from large sample volumes.
    Type: Grant
    Filed: October 3, 2017
    Date of Patent: September 14, 2021
    Inventors: Moran Bercovici, Govind Kaigala, Xander Frank Van Kooten, Nadya Ostromohov, Federico Paratore
  • Patent number: 11097272
    Abstract: According to an example, a microfluidic apparatus may include a fluid slot and a foyer that is in fluid communication with the fluid slot via a channel having a relatively smaller width than the foyer. The microfluidic apparatus may also include an electrical sensor to measure a change in an electrical field caused by a particle of interest in a fluid passing through the channel from the fluid slot to the foyer, an actuator to apply pressure onto fluid contained in the foyer, and a controller to receive the measured change in the electrical field from the electrical sensor, determine, from the received change in the electrical field, an electrical signature of the particle of interest, and control the actuator to control movement of the particle of interest based upon the determined electrical signature of the particle of interest.
    Type: Grant
    Filed: July 26, 2016
    Date of Patent: August 24, 2021
    Assignee: Hewlett-Packard Development Company, L.P.
    Inventors: Chantelle Domingue, Tod Woodford, Manish Giri, Matthew David Smith, George H Corrigan, III, Masoud Zavarehi, Joshua M. Yu
  • Patent number: 11092598
    Abstract: A chemically differentiated sensor array system includes a plurality of environmentally-gated transistors and an environmental gate, wherein the environmental gate includes a liquid solution and each environmentally-gated transistor includes a drain, a source, and a Carbon-based substrate channel, the drain electrically couples to a first location on the substrate channel, the source electrically couples to a second location on the substrate channel separated by a gap from the first location on the substrate channel, the environmental gate covers and contacts the substrate channel, a first insulating layer covers and separates the drain from the environmental gate, and a second insulating layer covers and separates the source from the environmental gate.
    Type: Grant
    Filed: May 8, 2017
    Date of Patent: August 17, 2021
    Assignee: Cardea Bio, Inc.
    Inventor: Brett Goldsmith
  • Patent number: 11079372
    Abstract: Polymers and conjugates comprising polymers are disclosed herein. In some embodiments, the conjugates disclosed are suitable for use as detection probes in immunohistochemical assays, including multiplex immunohistochemical assays.
    Type: Grant
    Filed: March 31, 2017
    Date of Patent: August 3, 2021
    Assignee: Ventana Medical Systems, Inc.
    Inventors: Lei Tang, Wenjun Zhang
  • Patent number: 11035795
    Abstract: A method of distinguishing between proteinaceous and non-proteinaceous particulates in a fluid sample includes the steps of acquiring a brightfield background image of a membrane filter, introducing a fluid sample onto the membrane filter, acquiring a brightfield image of filtered particles resting on the membrane filter, generating a particle mask based on the brightfield background image and the brightfield image of filtered particles, introducing a fluorescent dye onto the membrane filter, detecting fluorescence on the particle mask, and distinguishing between proteinaceous and non-proteinaceous particulates based on the detected fluorescence. A method for detecting other types of particles, such as polysorbate particles, silicone oil or protein monomers is also disclosed.
    Type: Grant
    Filed: December 24, 2019
    Date of Patent: June 15, 2021
    Assignee: Optofluidics, Inc.
    Inventors: Bernardo Cordovez, Brian DiPaolo, Gjergji Konica, Alexey Aprelev, Colby Ashcroft, Robert Hart
  • Patent number: 11028432
    Abstract: Methods, devices, and kits are provided for performing PCR and other thermal cycling reactions in <20 seconds per cycle, using induction heating.
    Type: Grant
    Filed: November 5, 2014
    Date of Patent: June 8, 2021
    Assignees: BioFire Diagnostics, LLC, University of Utah Research Foundation
    Inventors: David Jones, Carl T. Wittwer
  • Patent number: 10974243
    Abstract: A sensor using electrophoresis may include a microfluidic channel and electrodes on opposite sides of the microfluidic channel to generate an electric field across, or normal to, the channel. The electric field may be used to drive charged particles of material, particularly material suspended in fluid in the microfluidic channel, toward or away from the one of the electrodes. The electric field may be modulated to allow material to continue flowing through the microfluidic channel, to remove non-target material, or to measure another target material.
    Type: Grant
    Filed: July 26, 2017
    Date of Patent: April 13, 2021
    Assignee: Qorvo US, Inc.
    Inventor: Thayne L. Edwards
  • Patent number: 10933418
    Abstract: The present invention relates to biological sensing apparatus (12) which is configured to sense particles comprised in fluent material. The biological sensing apparatus (12) comprises particle sensing apparatus (32) comprised in an integrated circuit formed by a semiconductor fabrication process, the particle sensing apparatus being configured to sense an electrical property. The biological sensing apparatus further comprises a flow arrangement 30 configured to contain and provide for flow of fluent material. The particle sensing apparatus (32) is disposed relative to the flow arrangement (30) such that the particle sensing apparatus is operative to sense an electrical property of particles comprised in the fluent material as the fluent material flows through the flow arrangement.
    Type: Grant
    Filed: July 4, 2014
    Date of Patent: March 2, 2021
    Inventor: Keith Muir
  • Patent number: 10913062
    Abstract: Digital microfluidics system with electrodes attached to a PCB has control unit for manipulating liquid droplets by electrowetting, cartridge accommodation site for taking up a disposable cartridge having a working gap in-between two hydrophobic surfaces, and magnetic conduit/backing combination. A barrier element on an individual electrode of the PCB for narrowing the working gap. A disposable cartridge is positioned at the cartridge accommodation site, its flexible working film touching there and of the barrier element an uppermost surface. In the working gap and above a path of selected electrodes a liquid portion or liquid droplet with magnetically responsive beads moves by electrowetting on the electrode path until a magnetic field of the magnetic conduit is reached. The backing magnet is activated before and during the moving to thereby attract and remove magnetically responsive beads therefrom.
    Type: Grant
    Filed: September 1, 2016
    Date of Patent: February 9, 2021
    Assignee: TECAN TRADING AG
    Inventors: Thomas Perroud, Daniel Tran, Daniel Hoffmeyer, Tiffany Lay, Manjeet Dhindsa
  • Patent number: 10898894
    Abstract: Improved diagnostic assemblies are provided. More particularly, the present disclosure provides improved and highly advantageous chip based diagnostic assemblies configured to detect human diseases (e.g., cancer) and/or pathogens, and related methods of use. In exemplary embodiments, the present disclosure provides for consumable micro- or nano-fluidic chip based diagnostic assemblies having visual biosensors, with the diagnostic assemblies using continuous flow-based micro- or nano-fluidic channels and antibody-based immuno-complex designs. In certain embodiments, the diagnostic assembly includes a self-sustainable and operable chip (e.g., thumb-sized chip) that is configured to be deployed as a single use consumable with a direct all-or-none readout as an output to satisfy a point of screening method to screen a population.
    Type: Grant
    Filed: June 21, 2017
    Date of Patent: January 26, 2021
    Assignee: New Jersey Institute of Technology
    Inventors: Eon Soo Lee, Bharath Babu Nunna, K. Stephen Suh
  • Patent number: 10888863
    Abstract: This disclosure provides an apparatus and a method for quickly, efficiently and continuously fractionating biomolecules, such as DNAs and proteins based on size and other factors, while allowing imaging of the separated biomolecules as they are processed within the apparatus. The apparatus employs angled nanochannels to first preconcentrate and then separate like molecules. Its embodiments offer improved detection sensitivity and separation resolution over existing technologies and multiplexing capabilities.
    Type: Grant
    Filed: September 18, 2015
    Date of Patent: January 12, 2021
    Assignee: Massachusetts Institute of Technology
    Inventors: Sung Hee Ko, Jongyoon Han
  • Patent number: 10876088
    Abstract: Fluidic multiwell bioreactors are provided as a microphysiological platform for in vitro investigation of multi-organ crosstalks for an extended period of time of at least weeks and months. The disclosed platform is featured with one or more improvements over existing bioreactors, including on-board pumping for pneumatically driven fluid flow, a redesigned spillway for self-leveling from source to sink, a non-contact built-in fluid level sensing device, precise control on fluid flow profile and partitioning, and facile reconfigurations such as daisy chaining and multilayer stacking. The platform supports the culture of multiple organs in a microphysiological, interacted systems, suitable for a wide range of biomedical applications including systemic toxicity studies and physiology-based pharmacokinetic and pharmacodynamic predictions. A process to fabricate the disclosed bioreactors is also provided.
    Type: Grant
    Filed: February 6, 2017
    Date of Patent: December 29, 2020
    Inventors: Linda Griffith, David Trumper, Collin Edington, Gaurav Rohatgi, Duncan Freake, Luis Soenksen, Mohan Brij Bhushan
  • Patent number: 10866404
    Abstract: The disclosure provides an electrowetting device in which a sealing material is formed with satisfactory precision while maintaining a good adhesive property between both substrates. In a first hydrophobic layer (12) and a second hydrophobic layer (5), opening patterns (12a, 12b, 5a, and 5b) are provided, and an active substrate (7) and a common electrode substrate (2) are bonded together with a sealing material (14) provided in the opening patterns (12a, 12b, 5a, and 5b) such that a space is formed between the active substrate (7) and the common electrode substrate (2).
    Type: Grant
    Filed: March 22, 2017
    Date of Patent: December 15, 2020
    Inventors: Takeshi Hara, Akihiko Shibata
  • Patent number: 10852273
    Abstract: Methods, kits, and systems are provided for separating, immobilizing, and/or detecting analytes of one or more samples using dipsticks. A ‘dipstick’ is an object that can be embedded and subsequently removed from a separation medium, and to which analytes can be immobilized while the object is embedded in the separation medium. Examples of separation media include an electrophoresis gel of any format and a stationary phase for column chromatography. Embodiments of the present methods include applying a sample to a separation medium; separating analytes of the sample in the separation medium along a separation axis; immobilizing the analytes on a dipstick embedded in the separation medium; removing the dipstick from the separation medium; and detecting the analytes immobilized on the removed dipstick.
    Type: Grant
    Filed: September 21, 2018
    Date of Patent: December 1, 2020
    Assignee: Bio-Rad Laboratories, Inc.
    Inventor: William Strong
  • Patent number: 10816456
    Abstract: A reconfigurable point-of-care system, comprising an analysis device having one or more detection components to perform a diagnostic method on a sample, the sample being loaded on a microfluidic chip, wherein the analysis device provides identification information, an interface device coupled to the analysis device to provide a communication channel, and a reader unit coupled to the communication channel and having a processor to select the diagnostic method based on the identification information and reconfigure one or more components of the interface device based on the analysis device.
    Type: Grant
    Filed: October 19, 2016
    Date of Patent: October 27, 2020
    Inventors: Andre de Oliveira Botelho, Ricardo L. Ohta, Mathias B. Steiner, Jaione Tirapu Azpiroz
  • Patent number: 10807091
    Abstract: A method of driving an active matrix electro-wetting on dielectric (AM-EWOD) device comprises (i) setting a reference electrode to a first reference voltage; (ii) writing a set of data to array element electrodes of array elements of the device; and (iii) either (a) maintaining the voltages written to the array element electrodes until a time t0 or (b) re-writing the set of data N?1 times (where N?2). The reference electrode is then set to a second reference voltage different from the first reference voltage, and features (i) to (iii) are repeated. When the data are first written, there is a delay between the time when the voltage on the reference electrode is transitioned and the time when a given array element is next written with data.
    Type: Grant
    Filed: December 10, 2015
    Date of Patent: October 20, 2020
    Assignee: Sharp Life Science (EU) Limited
    Inventors: Benjamin James Hadwen, Jonathan Buse
  • Patent number: 10794895
    Abstract: A nanopore sensor is provided, including a nanopore disposed in a support structure. A fluidic passage is disposed between a first fluidic reservoir and the nanopore to fluidically connect the first fluidic reservoir to the nanopore through the fluidic passage. The fluidic passage has a passage length that is greater than the passage width. A second fluidic reservoir is fluidically connected to the nanopore, with the nanopore providing fluidic communication between the fluidic passage the second reservoir. Electrodes are connected to impose an electrical potential difference across the nanopore. At least one electrical transduction element is disposed in the nanopore sensor with a connection to measure the electrical potential that is local to the fluidic passage.
    Type: Grant
    Filed: February 4, 2016
    Date of Patent: October 6, 2020
    Assignee: President and Fellows of Harvard College
    Inventor: Ping Xie
  • Patent number: 10773214
    Abstract: A technology that provides various modular biomimetic microfluidic modules emulating varieties of microvasculature in body. These microfluidic-base capillaries and lymphatic Technology modules are constructed as multilayered-microfluidic microchannels of various shapes, and aspect ratios using diverse biocompatible microfluidic polymers. Then, various semipermeable membranes are sandwiched in between these multilayered microfluidic microchannels. These membranes have different chemical, physical characteristics and MWCO values. Consequently, this design will produce much smaller dimension channels similar to human vasculature to achieve biomimetic properties like of human organs and tissues. By interchanging microfluidic-layers or the membranes various diverse modules are designed that act as building blocks for constructing various medical devices, various forms of dialysis devices including albumin and lipid dialysis, water purification, bioreactors, bio-artificial organ support systems.
    Type: Grant
    Filed: March 3, 2016
    Date of Patent: September 15, 2020
    Inventor: Saeid Mordechai Nosrati
  • Patent number: 10767940
    Abstract: A method of operating a heat exchanger is disclosed in which an electric field is applied to a hydrophobic surface having condensed water droplets thereon to reduce a contact angle between the individual droplet surfaces and the hydrophobic surface, and to increase droplet surface energy to a second surface energy level. The electric field is removed to increase the contact angle between the individual droplet surfaces and the hydrophobic surface, and to reduce droplet surface energy to a third surface energy level. The third surface energy level is greater than the first surface energy level and greater than a surface energy level for a free droplet. A portion of the droplet surface energy is converted to kinetic energy to detach droplets from the hydrophobic surface. The detached droplets are removed from the heat rejection side fluid flow path.
    Type: Grant
    Filed: December 31, 2018
    Date of Patent: September 8, 2020
    Inventors: Abbas A. Alahyari, Miad Yazdani, Craig R. Walker