Boron Containing Patents (Class 205/469)
  • Detection of single base mutations and other variations in double stranded DNA by conformation-sensitive gel electrophoresis
    Patent number: 5874212
    Abstract: Simple methods of detecting single base pair mutations and other mutations in nucleic acid sequences are provided comprising generating a sample which may comprise heteroduplexes and homoduplexes and performing gel electrophoresis on the sample. The sample may be analyzed for one or more isolated high-melting domains prior to performance of gel electrophoresis. Kits are also provided.
    Type: Grant
    Filed: June 6, 1995
    Date of Patent: February 23, 1999
    Assignee: Thomas Jefferson University
    Inventors: Darwin J. Prockop, Matthew J. Rock, Arupa Ganguly