Chromatography Patents (Class 210/198.2)
  • Patent number: 11123656
    Abstract: The present invention relates to a method of separating and analyzing a mixture of oligonucleotides, including performing liquid chromatography using a column packed with a packing material obtained by fixing a diol to a surface of each of porous particles formed of a crosslinked organic polymer. According to this method, the oligonucleotides can be separated and analyzed with higher sensitivity compared to cases where columns having silica gel as a base material are used. In addition, the column can be washed with an alkaline solution.
    Type: Grant
    Filed: June 21, 2018
    Date of Patent: September 21, 2021
    Assignee: SHOWA DENKO K.K.
    Inventors: Junji Sasuga, Hirobumi Aoki, Yuzuru Kokido
  • Patent number: 11125730
    Abstract: A flow passage unit has a column for used in a liquid chromatograph and a support body that supports the column. The column has: a porous stationary phase; a porous pressure adjusting part disposed at least at the flow-in end of the stationary phase, a liquid entering the flow-in end, the pressure adjusting part being harder than the stationary phase; and a covering part that covers the stationary phase and pressure adjusting part. The support body has a first plate and a second plate that are mutually joined, the support body forming a column holding part and a liquid flow passage, the column holding part holding the column between the first plate and the second plate, the liquid flow passage communicating with the column holding part. Pressure applied from the first plate and second plate to the pressure adjusting part is higher than pressure applied to the stationary phase.
    Type: Grant
    Filed: December 7, 2017
    Date of Patent: September 21, 2021
    Assignee: ALPS ALPINE CO., LTD.
    Inventors: Yoshimune Suzuki, Setsuo Ishibashi, Yoshihiro Taguchi, Satoshi Kawamura, Junko Ito, Hiroyoshi Minaguchi
  • Patent number: 11110375
    Abstract: Microstructure separation filters are provided herein, as well as chromatography and other separation devices. An exemplary filter device includes a microstructure filter has a plurality of layers of alternating sacrificial and/or structural material which have been etched to create inlet channels and outlet channels. Adjacent ones of the inlet channels and the outlet channels are spaced apart from one another by cross channels that filter a fluid from the inlet channels to the outlet channels. The cross channels include filter features formed by etching away of a portion of the layers. The device also includes a housing configured to receive the microstructure filter.
    Type: Grant
    Filed: October 16, 2018
    Date of Patent: September 7, 2021
    Assignee: Imagine TF, LLC
    Inventor: Brian Edward Richardson
  • Patent number: 11110458
    Abstract: System, including methods and apparatus, for spacing droplets from each other and for detection of spaced droplets.
    Type: Grant
    Filed: February 3, 2014
    Date of Patent: September 7, 2021
    Assignee: Bio-Rad Laboratories, Inc.
    Inventors: George Carman, Thomas H. Cauley, III, David P. Stumbo
  • Patent number: 11112344
    Abstract: According to one embodiment, a particle measuring method is disclosed. The method includes irradiating a detection liquid with light. The detection liquid contains methyl salicylate. The method further includes converting scattered light from the detection liquid into an electric signal by using photoelectric conversion after irradiating the detection liquid with the light. The method further includes performing a particle measurement on the detection liquid by using the electric signal.
    Type: Grant
    Filed: March 3, 2020
    Date of Patent: September 7, 2021
    Assignees: Kioxia Corporation, Rion Co., Ltd.
    Inventors: Hiroshi Tomita, Hidekazu Hayashi, Eishi Shiobara, Kaoru Kondo, Takuya Tabuchi, Kazuna Bando, Sota Kondo
  • Patent number: 11097208
    Abstract: A vise for column, for attaching a plug to a column tube of a column for chromatography comprising the column tube and the plug to be attached to one end of the column tube, is provided. The vise comprises a column supporter configured to be able to support a part of the column for chromatography, a main body configured to be connected to the column supporter and extending from the column supporter by a predetermined distance, and a column presser configured to be supported at another end of the main body opposite to one end to which the column supporter is connected, and configured to be movable toward and away from the column supporter, and configured to be able to press the plug against the column tube.
    Type: Grant
    Filed: March 6, 2019
    Date of Patent: August 24, 2021
    Assignee: JNC CORPORATION
    Inventors: Naoki Yamanaka, Masami Todokoro
  • Patent number: 11092574
    Abstract: The invention relates to poly-amide bonded hydrophilic interaction chromatography (HILIC) stationary phases and novel HILIC methods for use in the characterization of large biological molecules modified with polar groups, known to those skilled in the art as glycans. The invention particularly provides novel, poly-amide bonded materials designed for efficient separation of large biomolecules, e.g. materials having a large percentage of larger pores (i.e. wide pores). Furthermore, the invention advantageously provides novel HILIC methods that can be used in combination with the stationary phase materials described herein to effectively separate protein and peptide glycoforms by eliminating previously unsolved problems, such as on-column aggregation of protein samples, low sensitivity of chromatographic detection of the glycan moieties, and low resolution of peaks due to restricted pore diffusion and long intra/inter-particle diffusion distances.
    Type: Grant
    Filed: April 2, 2015
    Date of Patent: August 17, 2021
    Assignee: Waters Technologies Corporation
    Inventors: Matthew A. Lauber, Stephan M. Koza, Pamela C. Iraneta, Kevin D. Wyndham
  • Patent number: 11090631
    Abstract: The present invention provides a method for hydrophobization of a hydrophilic material, the method including introducing a hydrophobic group into a hydroxyl group (—OH group) on a surface of the hydrophilic material. A method for hydrophobization of a hydrophilic material, the method comprising reacting a hydrophilic material to be hydrophobized with a hydrophobic group-containing silylating agent in presence of an amino acid as a reaction accelerator, to introduce a hydrophobic group-containing silyl group to a surface of the hydrophilic material. A hydrophobized silica gel column filler is produced by using the method. Further, a hydrophobized silica gel column is produced by filling a column with the hydrophobized silica gel column filler.
    Type: Grant
    Filed: March 6, 2020
    Date of Patent: August 17, 2021
    Assignee: SHIMADZU CORPORATION
    Inventors: Yukio Oikawa, Shigeyoshi Horiike, Hirotaka Koyama
  • Patent number: 11079360
    Abstract: Methods of chromatographic detection are provided that include the step of providing a chromatography instrument without an autosampler, an external device, a pressurized sample injection device and a chromatographic data system. The chromatographic instrument has a sample valve, a column manager and a solvent manager. The present methods further include the steps: injecting sample into the sample valve with a pressurized sample injection device; transmitting a signal from the chromatographic data system to the external device, receiving the signal by the external device, producing an event output with the external device, discharging solvent from the solvent manager into the sample valve, and discharging sample into the column for chromatographic detection.
    Type: Grant
    Filed: September 24, 2018
    Date of Patent: August 3, 2021
    Assignee: WATERS TECHNOLOGIES CORPORATION
    Inventors: Aaron D. Phoebe, Sylvain G. Cormier, Charles H. Phoebe, Jr.
  • Patent number: 11060974
    Abstract: An analyzing device includes a flow path plate having a flow path, through which a liquid flows, shaped like a rectangle, and having a light permeability, a light emitting unit having a light emitting lens and emitting light, and a light receiving unit including a light receiving lens and receiving the light, the analyzing device analyzing the liquid flowing inside the flow path plate, wherein the flow path plate has a pair of cutouts formed at an end face so as to face each other through a part of the flow path, wherein the pair of cutouts includes a first cutout in which the light emitting unit is disposed and a second cutout in which the light receiving unit is disposed.
    Type: Grant
    Filed: January 8, 2020
    Date of Patent: July 13, 2021
    Assignee: ALPS ALPINE CO., LTD.
    Inventor: Junko Ito
  • Patent number: 11058970
    Abstract: A vise for column, for attaching a plug to a column tube of a column for chromatography comprising the column tube and the plug to be attached to one end of the column tube, is provided. The vise comprises a column supporter configured to be able to support a part of the column for chromatography, a main body configured to be connected to the column supporter and extending from the column supporter by a predetermined distance, and a column presser configured to be supported at another end of the main body opposite to one end to which the column supporter is connected, and configured to be movable toward and away from the column supporter, and configured to be able to press the plug against the column tube.
    Type: Grant
    Filed: March 6, 2019
    Date of Patent: July 13, 2021
    Assignee: JNC CORPORATION
    Inventors: Naoki Yamanaka, Masami Todokoro
  • Patent number: 11041150
    Abstract: The present disclosure relates to fluidic systems and devices for processing, extracting, or purifying one or more analytes. These systems and devices can be used for processing samples and extracting nucleic acids, for example by isotachophoresis. In particular, the systems and related methods can allow for extraction of nucleic acids, including non-crosslinked nucleic acids, from samples such as tissue or cells. The systems and devices can also be used for multiplex parallel sample processing.
    Type: Grant
    Filed: August 1, 2018
    Date of Patent: June 22, 2021
    Assignee: Purigen Biosystems, Inc.
    Inventors: Lewis A. Marshall, Amy L. Hiddessen, Nathan P. Hoverter, Klint A. Rose, Juan G. Santiago, Matthew S. Munson, Janine Mok, Sean Arin, Yatian Qu, Andrew Lee, Michael Christopher DeRenzi
  • Patent number: 11035781
    Abstract: The present invention relates to an automated liquid transmission cell system capable of transferring solvents and samples to clean and fill the transmission cells. Three valves allow a plurality of system configurations to perform a variety of flushing and filling processes by changing the position of each valve. A plurality of tubes are connected to two of the valves such that changing the valve positions will redirect fluid transmission paths.
    Type: Grant
    Filed: January 31, 2019
    Date of Patent: June 15, 2021
    Assignee: The United States of America, as represented by the Secretary of the Navy
    Inventors: Michael L. Bishop, Christopher H. Clark, Gary G. Yeakley
  • Patent number: 11027220
    Abstract: A separation column for expanded bed adsorption comprises a column tube (2), a base (15) carrying an inlet rotor structure (6) for pumping in process liquid, and a top cap (3). The top cap (3) is conical in form, and has a peripheral flange 31 by which it is rigidly fixed to the top edge flange (22) of the column tube (2). The angle of the convergent interior surface (35) of the conical top cap (3) may be between 10 and 25°. A vortex-inhibitor component (8) projects down below the outlet structure (4) at the top of the cap, projecting into the operating space (15) of the column to inhibit rotation of liquid in the column interior. An expanded bed adsorption process is done with upward flow of liquid in the column through a bed of media particles.
    Type: Grant
    Filed: September 30, 2019
    Date of Patent: June 8, 2021
    Inventor: Martin John Hofmann
  • Patent number: 11022586
    Abstract: The invention provides an apparatus and system for the separation and optional analysis of the components of a sample of material, the apparatus and system comprising a cartridge comprising: at least one sample inlet port, at least one resin inlet port and a multiplicity of reagent and purge fluid input ports which are fluidically connected via a multiplicity of control valves to a multiplicity of chromatographic columns which are fluidically connected together in series; and a multiplicity of outlet ports wherein each outlet port additionally comprises an outlet valve which is adapted to control the flow of fluid through said outlet ports; wherein each of said multiplicity of chromatographic columns is aligned with one of said multiplicity of outlet ports so as to allow for fluid flow from said column through said outlet port. The system optionally additionally facilitates the analysis of the components.
    Type: Grant
    Filed: January 21, 2015
    Date of Patent: June 1, 2021
    Assignee: National Nuclear Laboratory Limited
    Inventors: Steven J. Stanley, Thomas O. Myers
  • Patent number: 11022593
    Abstract: Sampling devices for sampling an aqueous source (e.g., field testing of ground water) for multiple different analytes are described. Devices include a solid phase extraction component for retention of a wide variety of targeted analytes. Devices include analyte derivatization capability for improved extraction of targeted analytes. Thus, a single device can be utilized to examine a sample source for a wide variety of analytes. Devices also include an isotope dilution capability that can prevent error introduction to the sample analysis and can correct for sample loss and degradation from the point of sampling until analysis as well as correction for incomplete or poor derivatization reactions. The devices can be field-deployable and rechargeable.
    Type: Grant
    Filed: October 25, 2018
    Date of Patent: June 1, 2021
    Assignee: Savannah River Nuclear Solutions, LLC
    Inventors: Andrew J. Boggess, Stephen L. Crump, Thomas L. White
  • Patent number: 11009243
    Abstract: The techniques described herein provide systems and methods for controlling climate characteristics in a building. The control system of a climate management system may calculate a default airflow rate for each zone in the building based on a minimum permitted airflow rate of climate control equipment, a maximum permitted airflow rate of the climate control equipment, and the number of zones serviced by the climate control equipment. The control system may also adjust the default airflow rate for a zone to a customized airflow rate based on a user input. The control system may adjust the default airflow rate to the customized airflow rate if the customized airflow rate is greater than or equal to the minimum permitted airflow rate. The control system may adjust the default airflow rate to the minimum permitted airflow rate if the customized airflow rate is less than the minimum permitted airflow rate.
    Type: Grant
    Filed: May 22, 2018
    Date of Patent: May 18, 2021
    Assignee: Johnson Controls Technology Company
    Inventor: Shaun B. Atchison
  • Patent number: 10981085
    Abstract: A reaction assembly separating plant material from water includes a first annular filter element defining an axis. The first annular filter element is defined by an outer annular coil of flat wire and an optional second filter element is defined by an inner annular coil of flat wire, being generally helical in the axial direction. A cylindrically or frustoconical filter membrane is concentrically disposed between the first and second annular filter element. The filter membrane is porous having aperture size of less than a nano-particulate size of the plant material, but greater than a nano-particulate size of the water molecule. The second annular filter includes adjustable porosity for selectively preventing particles from reaching the filter membrane and selectively cleaning the membrane by reversed flow of water through the membrane. The assembly generates radial and distal flows and differential pressure forces, for use in high throughput industrial, agricultural and municipal facilities.
    Type: Grant
    Filed: January 16, 2020
    Date of Patent: April 20, 2021
    Inventor: E. Bayne Carew
  • Patent number: 10968252
    Abstract: Provided herein are methods of processing a fluid that include the use of one or both of a circuit system including a tangential flow filtration (TFF) unit and a circuit system including a tangential flow virus filtration (TFVF) unit. Also provided are integrated and continuous processes for manufacturing a therapeutic protein drug substance that include a step of processing a fluid including the recombinant therapeutic protein using any of the methods provided herein.
    Type: Grant
    Filed: August 15, 2017
    Date of Patent: April 6, 2021
    Assignee: Genzyme Corporation
    Inventors: Tarl Vetter, Michael Coolbaugh, Veena Warikoo, Konstantin Konstantinov
  • Patent number: 10960179
    Abstract: An applicator device for applying an agent to a target site with a malleable tip that can be cut to length with a pair of nursing dressing scissors or similar type of scissors and a malleable tip with a formable tube that that can be cut to length with a pair of nursing dressing scissors or similar type of scissors. The malleable tip is configured for passage of fluid therethrough and includes a malleable member. The malleable member is made of a soft metal that is strong enough to retain the shape of the tip after is has been bend into configuration, but soft and weak enough to be easily cut with a pair of nursing dressing scissors or the like.
    Type: Grant
    Filed: September 17, 2014
    Date of Patent: March 30, 2021
    Assignee: FERROSAN MEDICAL DEVICES, A/S
    Inventors: Flemming Reissig Jensen, Bettina Metzler, Martin Bue Bertelsen, Rasmus Kaastrup Torekov
  • Patent number: 10955393
    Abstract: A gas chromatograph with a removable column holder includes a column and a removable column holder. The column is configured for gas chromatography. The removable column holder houses the column. The removable column holder is configured to be removable from the gas chromatograph device with the column. The removable column holder houses only the column. The removable column holder is configured to allow removing and interchanging of the column within the removable column holder. The removable column holder is configured to protect the column within the column holder from mechanical damage and contamination due to handling. The removable column holder is configured to allow a user to install the column into the gas chromatograph without the need for tools. The removable column holder is also configured to reduce the possibility of error when installing the column.
    Type: Grant
    Filed: July 27, 2018
    Date of Patent: March 23, 2021
    Assignee: CEM CORPORATION, LUCIDITY DIVISION
    Inventors: Alyssa Whitney Sabolis, Michael John Collins, Jr., Michael Craig Davis, Daniel Charles Scheid, Brian Thomas Unites, Richard Michael Chapman
  • Patent number: 10949570
    Abstract: In an embodiment, a processing system includes a non-volatile memory, a hardware block, a protection circuit associated with the hardware block, and a password verification circuit. The non-volatile memory stores at least one reference password. The password verification circuit is configured to receive a password verification command, obtain a reference password, and test whether the passwords correspond. In case the passwords correspond, the password verification circuit generate an overwrite signal. The protection circuit is configured to receive a control command and selectively forward the control command to the associated hardware block as a function of the overwrite signal.
    Type: Grant
    Filed: July 18, 2018
    Date of Patent: March 16, 2021
    Assignee: STMICROELECTRONICS APPLICATION GMBH
    Inventor: Roberto Colombo
  • Patent number: 10941081
    Abstract: A cohesive granular material comprises granules made of a stiff substance and having a grain size in the range from 55 ?m to 2.0 mm; an elastomeric substance connecting the granules, a Young's modulus of the elastomeric substance being at maximum 0.5 times a Young's modulus of the stiff substance; and voids between the granules, the voids being interconnected and providing a fluid permeability to the cohesive granular material.
    Type: Grant
    Filed: January 16, 2019
    Date of Patent: March 9, 2021
    Assignee: MAX-PLANCK-GESELLSCHAFT ZUR FOERDERUNG DER WISSENSCHAFTEN E.V.
    Inventors: Arnaud Hemmerle, Lucas Goehring, Matthias Schroeter, Stephan Herminghaus
  • Patent number: 10933384
    Abstract: The object of the present invention is a new cryopolymerization process that provides crosslinked polymeric materials in the form of a macroporous gel (cryogel) capable of sequestering (neutralize) the anticoagulant heparin, its low molecular weight derivatives (LMWH and ULMWH) and heparinoids, from aqueous solutions, physiological solutions and biological fluids, such as whole blood, serum and plasma. A further object of the invention are also crosslinked polymeric materials in the form of a macroporous gel (cryogel) obtained by the cryopolymerization process of the invention that, thanks to said specific process, result to be comprised of varying proportions of HEMA and HEMA-R monomers. The molar ratio between the components (HEMA/HEMA-R) may vary between 99.9% HEMA:0.1% HEMA-R and 0.1% HEMA:99.9% HEMA-R.
    Type: Grant
    Filed: February 9, 2017
    Date of Patent: March 2, 2021
    Assignee: Consiglio Nazionale Delle Ricerche
    Inventors: Francesca Cunsolo, Tommaso Mecca, Rita La Spina, Fabiola Spitaleri
  • Patent number: 10927145
    Abstract: This application describes compositions, kits, and methods for removing contaminants from a protein preparation, for example a cell culture harvest comprising a recombinant protein. The methods described herein can enhance the performance of a later applied chromatographic purification step. The method generally involves contacting a protein preparation comprising a desired protein with a combination of allantoin, a cationic polymer, and a fatty acid, thereby causing the formation of solids and removal of the solids using a suitable process. Further treatment by contacting the treated sample with a chemically reactive surface further enhances results achieved by the first purification step.
    Type: Grant
    Filed: June 12, 2017
    Date of Patent: February 23, 2021
    Assignee: AGENCY FOR SCIENCE, TECHNOLOGY AND RESEARCH
    Inventors: Peter Stanley Gagnon, Yan Ling Phyllicia Toh, Wei Zhang
  • Patent number: 10916381
    Abstract: Forward and back electron transfer at molecule oxide interfaces are pivotal events in dye-sensitized solar cells, dye-sensitized photoelectrosynthesis cells and other applications. Disclosed herein are self-assembled multilayers as a strategy for manipulating electron transfer dynamics at these interfaces. The multilayer films are achieved by stepwise layering of bridging molecules, linking ions, and active molecule on an oxide surface. The formation of the proposed architecture is supported by ATR-IR and UV-Vis spectroscopy. Time-resolved emission and transient absorption establishes that the films exhibit an exponential decrease in electron transfer rate with increasing bridge length. The findings indicate that self-assembled multilayers offer a simple, straight forward and modular method for manipulating electron transfer dynamics at dye-oxide interfaces.
    Type: Grant
    Filed: July 14, 2015
    Date of Patent: February 9, 2021
    Assignee: The Florida State University Research Foundation. Inc.
    Inventors: Kenneth Hanson, Jamie Wang, Tanmay Banerjee, Omotola Ogunsolu
  • Patent number: 10913031
    Abstract: The present invention concerns an electronic safety system for an RO device which is configured to be used with at least one dialysis device (e.g., a hemodialysis or a peritoneal dialysis device). The system comprises the RO device, which is configured for the production of ultrapure water and which includes a sensor unit for collecting sensor data and whereby the RO device comprises an electronic data interface in order to send the sensor data collected by the sensor unit; and it also comprises an analysis unit which is configured to analyze a water sample with regards to safety requirements and for example with regard to contamination and to generate result data whereby the analysis unit also includes an analysis interface in order to send the generated result data in electronic form; and a network for the data exchange between the medical entities, for example between the RO device and the analysis unit.
    Type: Grant
    Filed: October 24, 2018
    Date of Patent: February 9, 2021
    Assignee: Fresenius Medical Care Deutschland GmbH
    Inventors: Christian Ruff, Thomas Pohl, Michael Wild
  • Patent number: 10894252
    Abstract: Provided is a fabrication method of a photocatalytic material in which a single layer of a carbon-based participate is formed on a surface of each of titanium dioxide particle. The method includes (a) loading titanium dioxide particles into an electric furnace comprising a mechanism for rotating a core tube; (b) heating an inside of the core tube of the electric furnace into which the titanium dioxide particles have been loaded to a temperature of not less than 400° C. and not more than 800° C., while an inert gas is introduced into the inside of the core tube; (c) supplying a hydrocarbon gas to the inside of the core tube in addition to the inert gas; and (d) performing a thermal CVD on each of the titanium dioxide particles in a fluidized state inside the core tube, while the core tube is rotated, to form a single layer of a carbon-based precipitate containing graphene on a surface of each of the titanium dioxide particles. A photocatalyst material is provided.
    Type: Grant
    Filed: July 16, 2019
    Date of Patent: January 19, 2021
    Assignee: PANASONIC INTELLECTUAL PROPERTY MANAGEMENT CO., LTD.
    Inventors: Kohei Hara, Shunsuke Kori, Yasuhiro Hashimoto, Daisuke Ino
  • Patent number: 10895009
    Abstract: The invention provides metal liquid chromatography components with uniformly coated internal surfaces and methods for achieving the same. The invention addresses the problem of corrosion or interference of metal components in the flow path for LC analyses in which the sample interacts with metal ions or surfaces. The invention also alleviates the difficulties in coating very long metal tubes and very small metal channels with an inert, continuous coating that adheres well to metal surfaces. The metal flow path is rendered inert by the coating, and thus compatible with bioanalytical separations, for example, by using a vapor phase deposition process to coat the inner surfaces with a coating that continuously covers all metal surfaces in the flow path.
    Type: Grant
    Filed: June 27, 2019
    Date of Patent: January 19, 2021
    Assignee: Agilent Technologies, Inc.
    Inventors: Elizabeth Carr, Karen L. Seaward, Kevin P. Killeen
  • Patent number: 10875008
    Abstract: The present invention provides a separation material that comprises porous polymer particles comprising a styrene-based monomer as a monomer unit; and a coating layer comprising a macromolecule having hydroxyl groups, which covers at least a portion of the surface of the porous polymer particles, and the separation material has a 5% compressive deformation modulus of 100 to 1,000 MPa, and has a mode diameter in the pore size distribution of 0.1 to 0.5 ?m.
    Type: Grant
    Filed: January 19, 2016
    Date of Patent: December 29, 2020
    Assignee: SHOWA DENKO MATERIALS CO., LTD.
    Inventors: Masaru Watanabe, Tomoko Higashiuchi, Fumihiko Kawauchi, Yasushi Gotoh, Michio Butsugan, Ryoichi Nakanishi
  • Patent number: 10873071
    Abstract: A negative electrode active material of the present invention includes a core containing silicon-based nanoparticles and polymer carbides distributed on the nanoparticles, wherein the core has a size of 30-300 nm, and such a negative electrode active material is prepared using a method including dispersing a suspension in which silicon-based nanoparticles and water-soluble polymer are added to a solvent using ultrasonic waves; and preparing a core including the silicon-based nanoparticles having the polymer carbides on the surface by carbonizing the water-soluble polymer. As a result, a negative electrode active material having a significantly low volume expansion rate compared with general non-carbon-based negative electrode active materials, and having excellent electric conductivity may be provided.
    Type: Grant
    Filed: November 27, 2015
    Date of Patent: December 22, 2020
    Inventors: Hyun Chul Kim, Yong Ju Lee, Yoon Ah Kang, Eun Kyung Kim
  • Patent number: 10866217
    Abstract: A liquid chromatograph includes a plurality of columns, fluid supply units each configured to feed a predetermined solution in parallel with inlet side flow paths of the plurality of columns, a sample injection unit capable of selectively injecting a sample into one of the inlet side flow paths of the plurality of columns, and a flow path switching unit disposed on exit sides of the plurality of columns and configured to selectively connect one of outlet side flow paths of the plurality of columns to a flow path connected to a detector arranged on a downstream side of the flow path switching unit and connect outlet side flow paths of other columns to a waste liquid flow path. The liquid chromatograph may improve a throughput by shortening a time required for a series of measurements in which a sample is measured under a plurality of conditions by alternatively using a plurality of columns.
    Type: Grant
    Filed: March 27, 2018
    Date of Patent: December 15, 2020
    Assignee: Shimadzu Corporation
    Inventor: Kenichi Yasunaga
  • Patent number: 10864461
    Abstract: The invention relates to a chromatography method in which a gaseous, liquid or supercritical mobile phase containing species to be separated is circulated through a packing, said packing comprising: —a plurality of capillary ducts extending in the packing between an upstream face through which the mobile phase enters the packing and a downstream face through which the mobile phase leaves the packing, and —a continuous medium permeable to molecular diffusion extending between said ducts, comprising a porous organic gel or an organic liquid and including at least one network of connected pores, the size of which is greater than two times the molecular diameter of at least one species to be separated and opening to the ducts, so as to give said at least one species a diffusive path between said ducts. The invention also relates to a packing for the implementation of such a method and a method for manufacturing such a packing.
    Type: Grant
    Filed: September 29, 2015
    Date of Patent: December 15, 2020
    Inventor: Francois Parmentier
  • Patent number: 10859544
    Abstract: A secondary ultrasonic nebulisation device is disclosed comprising: a liquid sample delivery capillary; a sample receiving surface arranged for receiving a liquid sample from the capillary; and an ultrasonic transducer configured for oscillating the surface so as to nebulise the liquid sample received thereon, wherein the device is configured such that the oscillations of the surface by the ultrasonic transducer cause charged droplets and/or gas phase ions to be generated from the sample.
    Type: Grant
    Filed: September 24, 2019
    Date of Patent: December 8, 2020
    Assignee: Micromass UK Limite
    Inventors: Stevan Bajic, David S. Douce, Gordon A. Jones
  • Patent number: 10859476
    Abstract: A liquid chromatography having an on-line cleaning function, comprising a first flow channel (L3), a second flow channel (L21), an analysis flow channel (L22) and waste liquid flow channels, further comprising a cleaning flow channel (L25), a direction switch valve (V1) and a multi-flow channel switch valve (V2), etc. The liquid chromatography changes the flow path of the liquid by changing the communication relationship between the two-position switch valves, thus realizes the on-line cleaning function for a first chromatographic column (C1), a middle chromatographic column (C2), a filter or a protector (B2) respectively, and realize the simultaneous on-line cleaning function for the first chromatographic column (C1) and the middle chromatographic column (C2).
    Type: Grant
    Filed: November 27, 2014
    Date of Patent: December 8, 2020
    Assignee: HUNAN DEMETER INSTRUMENTS CO., LTD.
    Inventor: Feng Wang
  • Patent number: 10852278
    Abstract: The present invention provides a detector and method for detecting substances in complex mixtures. The detector includes a microfabricated preconcentrator, a separation column with an on-chip thermal conductivity detector, a controller for controlling flow and thermal management and a user interface. The thermal conductivity detector includes a first resistor located at an inlet of the separation column and a second resistor located at an outlet of the separation column.
    Type: Grant
    Filed: August 3, 2018
    Date of Patent: December 1, 2020
    Assignee: Virginia Tech Intellectual Properties, Inc.
    Inventors: Masoud Agah, Muhammad Akbar, Apoorva Garg, Leyla Nazhandali, Hamza Shakeel
  • Patent number: 10849927
    Abstract: Covalently cross-linked copolymers are described herein. More specifically, polysaccharide-polyamine copolymeric matrices or structures and cationic copolymeric matrices are described herein. The polysaccharide-polyamine copolymers, when protonated, can form cationic copolymeric matrices having exceptionally high densities of cationic sites. In one form, the covalently cross-linked copolymers provide a three-dimensional structure, especially when hydrated.
    Type: Grant
    Filed: March 18, 2016
    Date of Patent: December 1, 2020
    Assignee: HOWARD UNIVERSITY
    Inventors: James W. Mitchell, Dazhi Yang
  • Patent number: 10845345
    Abstract: A gas chromatograph is provided with a main body, a detector 4, a display 11, and a display processing unit 132. The display 11 is provided in the main body. Further, a touch panel type display screen is included in the display 11. The display processing unit 132, based on a detection signal from the detector 4, displays a chromatogram in a first graph region in the display screen, and, based on a touch operation performed by an operator with respect to the first graph region, modifies the display in the first graph region.
    Type: Grant
    Filed: December 28, 2015
    Date of Patent: November 24, 2020
    Assignee: SHIMADZU CORPORATION
    Inventor: Masashi Yamane
  • Patent number: 10829758
    Abstract: The present invention relates to a highly automatable method for isolation and/or purification of nucleic acids from a biological sample, which is particularly suitable for nucleic acids-shorter than 250 bp and can be performed without a proteolytic pre-digestion step in an automated system, preferably a cartridge-based system. In a further aspect, the present invention also provides automated nucleic acid detection methods based on said isolation and/or purification method, as well as buffers and kits to be used in performing said methods.
    Type: Grant
    Filed: June 9, 2016
    Date of Patent: November 10, 2020
    Assignee: BIOCARTIS, NV
    Inventors: Geert Meersseman, Klaas DeCanniere
  • Patent number: 10823712
    Abstract: A method of performing a chromatographic separation includes modulating a portion of a flowstream to a trap column to retain at least one analyte from the flowstream on the trap column. A flow of a modifier is provided through the trap column to generate an elution comprising the at least one analyte. A flow of a compressible fluid-based chromatography (CFC) mobile phase or CFC solvent is merged with the elution from the trap column to generate a diluted elution. Carbon dioxide may be used as the CFC solvent or as a component of the CFC mobile phase. The diluted elution is provided to a CFC column where at least one analyte is focused at a head of the CFC column. Examples of a flowstream that may be used include an eluent from a chromatography column or a fluid flow from an extraction system.
    Type: Grant
    Filed: December 21, 2017
    Date of Patent: November 3, 2020
    Assignee: WATERS TECHNOLOGIES CORPORATION
    Inventors: Michael O. Fogwill, Isabelle François
  • Patent number: 10816489
    Abstract: A monitoring device is provided for analytical measurement of reaction fluid produced in a reaction vessel in a spectrometer with a monitoring cell. The distribution apparatus includes at least four supply and return lines that open into the distribution apparatus, wherein the distribution apparatus comprises a distribution device for distributing reaction fluid to the supply and return lines. The distribution apparatus comprises a distribution vessel in which the distribution device and an electrically controllable pump device for pumping of the reaction fluid are provided, wherein the distribution device comprises an electrically controllable valve device for distributing the reaction fluid to the lines that open into the distribution vessel.
    Type: Grant
    Filed: November 14, 2017
    Date of Patent: October 27, 2020
    Assignee: BRUKER BIOSPIN GMBH
    Inventors: Ernst Ulrich Braumann, Martin Hofmann
  • Patent number: 10809259
    Abstract: Methods for detecting and quantifying one or more microcystin compounds in a sample are described. The methods may include a preconcentration step, and generally utilize an LC-MS or LC-MS/MS analysis with an Orbitrap Fusion mass spectrometer or a QqQ mass spectrometer. The methods provide excellent recoveries and limits of quantification of microcystins.
    Type: Grant
    Filed: July 12, 2018
    Date of Patent: October 20, 2020
    Assignee: The University of Toledo
    Inventors: Dragan Isailovic, Dilrukshika Palagama, David Baliu-Rodriguez, Bruce S. Levison, David J. Kennedy, Steven T. Haller, Kenneth Hensley
  • Patent number: 10801928
    Abstract: A fluid sample collection system for directly collecting a fluid sample from a fluid source without exposing the fluid sample to an ambient environment that surrounds the fluid sample collection system includes a fluid collector including (i) a sample vial that is configured to retain fluid from the fluid source, the sample vial including a sample vial body and a vial cap that is selectively coupled and sealed to the sample vial body; (ii) a collector body that defines a passenger vial chamber, the sample vial being positioned at least partially within the passenger vial chamber during collection of the fluid; and (iii) a cap access facilitator that is configured to engage a portion of the sample vial to enable a user to selectively couple the vial cap to the sample vial body to seal the sample vial so that the fluid is retained within the sample vial.
    Type: Grant
    Filed: February 9, 2018
    Date of Patent: October 13, 2020
    Assignee: BESST, Inc.
    Inventors: Noah R. Heller, Jacob Aman, Gaunt Murdock, Miles Koehler
  • Patent number: 10802000
    Abstract: A method of calibrating, a chromatography system is described. The method includes injecting a standard into a chromatographic separator. The standard including a first analyte having a first calibrant concentration and a second analyte having a second calibrant concentration. The standard can be separated in the chromatographic separator and measured with a detector. The method automatically identifies whether the first peak corresponds to the first analyte or the second analyte and whether the second peak corresponds to the first analyte or the second analyte, based on either an area or a peak height of the first peak and the second peak, and a ratio based on the first calibrant concentration and the second calibrant concentration.
    Type: Grant
    Filed: March 15, 2013
    Date of Patent: October 13, 2020
    Assignee: Dionex Corporation
    Inventor: Christopher A. Pohl
  • Patent number: 10802003
    Abstract: A column-sealing tool is provided for use during decoupling of a capillary column from a gas chromatography-mass spectrometry (GC-MS) system. The column-sealing tool includes an engagement structure for removably securing the column-sealing tool within a high vacuum enclosure of the GC-MS system, at a location between a high vacuum end of a transfer line and a mass analyzer of the GC-MS system. Additionally, the column-sealing tool includes a sealing element that is actuatable between a non-sealing position and a sealing position. During use the sealing element forms a secondary vacuum seal with the first end of the transfer line when the column-sealing tool is inserted into the GC-MS system and when the sealing element is in the sealing position.
    Type: Grant
    Filed: December 14, 2018
    Date of Patent: October 13, 2020
    Assignee: THERMO FINNIGAN LLC
    Inventors: James M. Hitchcock, George B. Guckenberger
  • Patent number: 10799813
    Abstract: A chromatography system includes a first chromatography column for receiving and separating a flow stream, a plurality of traps configured to trap a plurality of distinct flow segments exiting the first chromatography column during separation of the flow stream, and a second chromatography column operatively associated with the plurality of traps for receiving and separating the distinct flow segments. The system can include at-column dilution at trapping and separating stages thereof. A chromatography method for operating the chromatographic system includes measuring a plurality of time segments corresponding to a plurality of peaks of a fluid sample flowing through the first chromatographic column, and sequentially fluidly coupling the plurality of distinct flow segments with the corresponding plurality of traps during time segments corresponding to the plurality of peaks.
    Type: Grant
    Filed: January 25, 2017
    Date of Patent: October 13, 2020
    Assignee: Waters Technologies Corporation
    Inventors: Amanda B. Dlugasch, Thomas E. Wheat, Daniel Root, Jean-Michel Plankeele, Isabelle Francois, Edward R. Aig
  • Patent number: 10793592
    Abstract: The present invention relates to the purification of target molecules like recombinant and/or biotherapeutic proteins. Activated carbon can be used to remove leachables and/or extractables resulting from disposable equipment employed in the process.
    Type: Grant
    Filed: October 20, 2015
    Date of Patent: October 6, 2020
    Assignee: Merck Patent GmbH
    Inventors: Romas Skudas, Klaus Adrian, Bianca Edlemann, Sven Andrecht, Wilson Moya
  • Patent number: 10788468
    Abstract: A clamp for chromatography columns has a first seal with a first opening a movable seal with a second opening and a movable coupler. The movable coupler has first and second coupler seals with communicating third and fourth openings. The clamp is arranged for pressing a first chromatography column between the first seal and the first coupler seal and for pressing a second chromatography column between the movable seal and the second coupler seal, such that the first opening fluidly communicates with the second opening through the first chromatography column, the third and fourth openings and the second chromatography column.
    Type: Grant
    Filed: January 17, 2020
    Date of Patent: September 29, 2020
    Assignee: BIO-RAD LABORATORIES, INC.
    Inventor: David Lerner
  • Patent number: 10788405
    Abstract: Systems and methods for inline and automatic dilution of chemicals of interest for speciation and subsequent analysis by ICP spectrometry are described. A system embodiment includes a first valve to receive a sample into a holding loop; a plurality of syringe pumps coupled to the first valve to deliver an inline diluted sample from the first valve; and a second valve coupled to the first valve to receive the inline diluted sample from the first valve into a sample holding loop coupled to the second valve, the second valve configured to couple to at least one of an eluent source or a carrier fluid source to receive at least one of an eluent fluid or a carrier fluid to transfer the inline diluted sample from the sample holding loop to a speciation column to separate one or more species from the inline diluted sample.
    Type: Grant
    Filed: March 26, 2019
    Date of Patent: September 29, 2020
    Assignee: ELEMENTAL SCIENTIFIC, INC.
    Inventors: Michael Paul Field, Daniel R. Wiederin, Patrick Sullivan
  • Patent number: 10774957
    Abstract: Described is a mobile phase fitting having reduced corrosion and erosion. The fitting includes a coupling body, compression screw, compression member and gasket. The coupling body has a threaded bore at one end, a tapered cavity, a narrow bore and a fluid channel. The compression screw has an axial bore to receive a tube and a threaded outer surface in engagement with the threaded bore of the coupling body. The compression member is disposed in the tapered cavity and has an axial opening to pass the tube and a tapered surface to engage a surface of the tapered cavity. The gasket is disposed in the narrow bore and has one surface in contact with the first internal surface and has a parallel surface to receive an end face of the tube. Mobile phase flows along a path that includes the tube, an opening in the gasket and the fluid channel.
    Type: Grant
    Filed: July 30, 2018
    Date of Patent: September 15, 2020
    Assignee: WATERS TECHNOLOGIES CORPORATION
    Inventors: Jonathan L. Belanger, Sylvain Cormier, Steven D. Trudeau, Paul Keenan, Daniel J. McCormick