Chromatography Patents (Class 210/198.2)
  • Patent number: 11480505
    Abstract: A kit for extracting drug residues from livestock or poultry aquatic products according to the present disclosure includes a pipe, a first powder mixture layer and a second powder mixture layer. The pipe has an output port at the bottom thereof and an input port at the top thereof for inputting a sample solution. The first powder mixture layer is in the form of powder and filled in the pipe. The first powder mixture layer contains anhydrous sodium sulfate powder and sodium chloride powder. The second powder mixture layer is in the form of powder and filled in the pipe. The second powder mixture layer is located below the first powder mixture layer and above the output port. The second powder mixture layer contains anhydrous sodium sulfate powder and C18 powder. The present disclosure further provides a method of obtaining a primary test liquid from livestock or poultry aquatic products using the above kit.
    Type: Grant
    Filed: October 18, 2019
    Date of Patent: October 25, 2022
    Assignee: GREAT ENGINEERING TECHNOLOGY CORPORATION
    Inventors: Hung-Ta Hua, Yi-Jia Ku
  • Patent number: 11479644
    Abstract: The invention provides a polymerizable composition and a hydrogel obtained or obtainable from the polymerizable composition. The polymerizable composition comprises cucurbituril and a first monomer having a guest for the cucurbituril, wherein the total monomer concentration, Cmon, within the polymerizable composition is at least 0.5 M. The composition may be an aqueous composition.
    Type: Grant
    Filed: October 13, 2017
    Date of Patent: October 25, 2022
    Inventors: Oren Scherman, Ji Liu
  • Patent number: 11478524
    Abstract: The present disclosure relates to methods of modifying complex extracts such that components or mixtures of components are selectively removed or added, thus providing a complex mixture that does not naturally occur with a refined or a tuned therapeutic or nutraceutical effect. In various aspects, the complex extract can be an extract obtained from one or more plants, e.g., an extract obtained from green tea leaves. The present disclosure pertains to compositions obtained by the disclosed methods, nutraceutical compositions comprising same, pharmaceutical compositions comprising same, and methods of treating various conditions, including physiological dysfunctions associated with elevated reactive oxygen species and/or inflammatory molecule, e.g., TNF?, expression using same. This abstract is intended as a scanning tool for purposes of searching in the particular art and is not intended to be limiting of the present disclosure.
    Type: Grant
    Filed: July 31, 2018
    Date of Patent: October 25, 2022
    Assignee: UNIVERSITY OF SOUTH FLORIDA
    Inventor: Bill J. Baker
  • Patent number: 11464779
    Abstract: The present invention belongs to the pharmaceutical field, and in particular, it relates to a pharmaceutical formulation of palbociclib and a preparation method thereof. The pharmaceutical formulation comprises palbociclib, an acidic auxiliary material, and optionally a hydrophilic high-molecular material, which has better solubility and in vitro dissolution property as compared with the conventional formulation and can be used for enhancing in vivo absorption and bioavailability of palbociclib.
    Type: Grant
    Filed: September 17, 2020
    Date of Patent: October 11, 2022
    Assignee: Shenzhen Pharmacin Co., Ltd.
    Inventors: Zeren Wang, Jun Xu, Long Qu
  • Patent number: 11460452
    Abstract: Embodiments of the present disclosure may assist in managing, storing, using, and re-using dedicated chromatography column bed supports. For example, devices and systems disclosed herein may include reusable cases for storing dedicated column bed supports when the column bed supports are not in use. Such cases may include one or more dividers and inserts configured to fit column bed supports, and may be designed and built specifically to ensure efficient, secure storage of column bed supports while minimizing risk of damaging or contaminating them. Methods disclosed herein may include performing a particular chromatography operation in a column, removing the column bed supports from the column, storing the column bed supports in a column bed support storage case, and installing another set of column bed supports in the column for another chromatography operation.
    Type: Grant
    Filed: November 29, 2018
    Date of Patent: October 4, 2022
    Assignee: Regeneron Pharmaceuticals, Inc.
    Inventors: Kevin Kent, Joseph Gallea, Benjamin Binga
  • Patent number: 11454618
    Abstract: A system and method are disclosed for analyzing samples, which includes a spectrometry system for detecting components of a sample; a gas chromatography column for separating the components of a sample; a first sample unit for receiving a first sample from a sample source; and a second sample unit for receiving a second sample from a sample source. Each sample loop unit allows independent processing of samples in preparation for analysis.
    Type: Grant
    Filed: July 9, 2019
    Date of Patent: September 27, 2022
    Assignee: MLS ACQ, INC.
    Inventors: Martin L. Spartz, Anthony S. Bonanno, Peter P. Behnke
  • Patent number: 11434464
    Abstract: The disclosure provides a filtration system for a cell culture apparatus and a method of cell culture. The filtration system comprises a bioreactor vessel and two or more alternating tangential flow (ATF) filters connected in parallel. A failure in either filter is detected by an in-line sensor, and an automated response system functions to sequester the malfunctioning filter by stopping the flow of liquid media through the filter. Media flow through the remaining operable filters can be increased so that the rate of perfusion through the bioreactor remains relatively unchanged. Such a system may prevent issues that arise from ATF filter failures in conventional perfusion bioreactors, thereby improving the long-term viability of cell cultures.
    Type: Grant
    Filed: November 11, 2021
    Date of Patent: September 6, 2022
    Assignee: Genzyme Corporation
    Inventors: Jiuyi Lu, Jason Walther, Jonathan Wang, Kevin Victor Chen
  • Patent number: 11422117
    Abstract: A flow cell for a detector in a liquid chromatography system includes a flow cell body, an inlet, an outlet, at least two windows situated on opposing sides of the flow cell body, which are transparent for light, and at least two electrodes configured for a conductivity measurement. A detection channel is formed within said flow cell body, fluidly connecting said inlet and outlet, includes an optical path situated in between said two windows such that a light absorption measurement may be performed for a liquid passing through the detection channel, and a conductivity path formed by at least partially drilling through said electrodes to allow for a physical contact of a liquid passing through the detection channel. A detector or liquid chromatography system including such a flow cell as well as uses of the flow cell, detector or liquid chromatography system are also described.
    Type: Grant
    Filed: August 30, 2019
    Date of Patent: August 23, 2022
    Assignee: KNAUER Wissenschaftliche Geräte GmbH
    Inventors: Detlef Halle, Michael Melnykov, Karl Zenichowski
  • Patent number: 11406915
    Abstract: Chromatography method in which a gaseous, liquid or supercritical mobile phase containing species to be separated is circulated through a packing, said packing being characterized in that: —it comprises a plurality of capillary ducts extending in the packing between an upstream face through which the mobile phase enters the packing and a downstream face through which the mobile phase leaves the packing—the material of the walls comprises a first population of connected pores, providing passages from one duct to the next enabling molecular diffusion to take place between adjacent ducts, pores having a mean diameter (dpore) of greater than 2 times the molecular diameter of at least one species to be separated—the diameter of the ducts is less than 50 ?m.
    Type: Grant
    Filed: September 29, 2015
    Date of Patent: August 9, 2022
    Assignee: SEPARATIVE
    Inventor: Francois Parmentier
  • Patent number: 11402359
    Abstract: The present invention describes a method of operating a fractionation collector in chromatography, and a chromatograph system for carrying out the method, wherein a solvent composition is pumped into a chromatography column, which contains at least a portion of a first solvent that is liquid under normal conditions and contains a portion of a second solvent that is gaseous at normal conditions, and the composition flowing out of the chromatography column is at least partially introduced into a detector, wherein a composition containing the first solvent, after leaving the chromatography column, is introduced into a collection vessel of the fraction collector in dependence of the detector signal, and the amount of liquid that is introduced into a collecting vessel of the fraction collector is selected in dependence of the portion of said first solvent.
    Type: Grant
    Filed: September 3, 2018
    Date of Patent: August 2, 2022
    Inventor: Alexander Bozic
  • Patent number: 11402357
    Abstract: The invention relates to containers or bags for chromatographic media and methods of packing chromatography columns using such containers. The bags may be used for storing and/or transporting chromatographic media and can be inserted directly into the chamber of a chromatography column in readiness for use.
    Type: Grant
    Filed: July 12, 2016
    Date of Patent: August 2, 2022
    Assignee: Cytiva Sweden AB
    Inventor: Klaus Gebauer
  • Patent number: 11391706
    Abstract: A chromatography system includes a modulator, a chromatograph, a cooler, and a detector. The chromatograph is connected to the modulator by a first heated transfer line. The detector is connected to the modulator by a second heated transfer line. The cooler is connected to the modulator. The modulator is arranged outside of the chromatograph.
    Type: Grant
    Filed: December 13, 2017
    Date of Patent: July 19, 2022
    Assignee: LECO Corporation
    Inventor: Mark Firmer Merrick
  • Patent number: 11389747
    Abstract: The invention provides filters and methods of assembling filters. In an exemplary embodiment, the filter includes a porous element, a compression element, and a housing. The compression element can be configured to receive the porous element, thereby forming an assembly. For example, the compression element can receive the porous element in a slip-fit relationship. The housing can have an opening formed therein that is configured to receive the assembly. In some embodiments, the assembly can be retained within the opening when the assembly is received therein. For example, the opening can receive the assembly in a press-fit relationship.
    Type: Grant
    Filed: June 6, 2014
    Date of Patent: July 19, 2022
    Assignee: WATERS TECHNOLOGIES CORPORATION
    Inventors: Mathew H. Delano, Jonathan Belanger, Raymond P. Fisk
  • Patent number: 11340198
    Abstract: The present disclosure relates to methodologies, systems, and apparatus for controlling fluid flow within a chromatography system. The chromatography system includes a mobile phase pump configured to pump a liquid mobile phase through a column and a restrictor positioned downstream of the column and upstream of a detector. The system also includes a valve configured to operate in at least two positions. In a first position, the valve is configured to direct the output of the column to bypass the valve and reach the detector, while in the second position the valve directs the output of the column to waste.
    Type: Grant
    Filed: May 29, 2019
    Date of Patent: May 24, 2022
    Assignee: Waters Technologies Corporation
    Inventors: Michael O. Fogwill, Theodore A. Dourdeville
  • Patent number: 11331596
    Abstract: A chromatography system includes a first chromatography column for receiving and separating a flow stream, a plurality of traps configured to trap a plurality of distinct flow segments exiting the first chromatography column during separation of the flow stream, and a second chromatography column operatively associated with the plurality of traps for receiving and separating the distinct flow segments. The system can include at-column dilution at trapping and separating stages thereof. A chromatography method for operating the chromatographic system includes measuring a plurality of time segments corresponding to a plurality of peaks of a fluid sample flowing through the first chromatographic column, and sequentially fluidly coupling the plurality of distinct flow segments with the corresponding plurality of traps during time segments corresponding to the plurality of peaks.
    Type: Grant
    Filed: September 16, 2020
    Date of Patent: May 17, 2022
    Assignee: Waters Technologies Corporation
    Inventors: Amanda B. Dlugasch, Thomas E. Wheat, Daniel Root, Jean-Michel Plankeele, Isabelle Francois, Edward R. Aig
  • Patent number: 11333639
    Abstract: Systems, apparatuses, kits, and methods for purification and analysis of analytes having a broad range of hydrophobicities by liquid chromatography-mass spectrometry (LC-MS). Using one set of liquid chromatography columns, one set of mobile phase buffers, and, optionally, a single ionization method (e.g., electrospray ionization), a wide range of analytes can be purified and analyzed on a liquid chromatography-mass spectrometry (LC-MS) system. LC-MS purification and analysis of analytes having a broad range of partition coefficients is accomplished by selecting LC run parameters and MS system parameters that are particular to different classes of analytes without having to make column or buffer changes or any other hardware configuration changes to the LC-MS system.
    Type: Grant
    Filed: March 12, 2020
    Date of Patent: May 17, 2022
    Assignee: Cohesive Technologies, Inc.
    Inventors: Joseph L. Herman, Robert Dewitte, Dayana Argoti
  • Patent number: 11320409
    Abstract: There is provided an integrated system for liquid separation, such as LC, CE, affinity chromatography, and ion exchange chromatography, comprising a column and end-fittings embedded in a plastic material, such as a thermoplastic polymer. The system may further comprise an electrospray emitter directly connected with the outlet of the column, wherein a substantial part of the emitter is covered with the polymer material. There is also provided a method by which a separation column along with the accompanying end fittings for connection with adjacent liquid conduits is embedded in a polymer matrix. This configuration e.g. ensures that the factory-made, correct attachment of the fittings to the column is preserved (since the matrix prevents further user intervention, accidental or otherwise). Accordingly, the responsibility for the correct attachment of the fittings is shifted from the end user to the manufacturer.
    Type: Grant
    Filed: February 17, 2020
    Date of Patent: May 3, 2022
    Assignee: PROXEON BIOSYSTEMS ApS
    Inventors: Ole Vorm, Soren Theodorsen, Peter Aagaard Nielsen
  • Patent number: 11305272
    Abstract: An ion exchanger filled cartridge accommodated inside an accommodating container of a metal removing column and in which an ion exchanger is filled is provided. The ion exchanger filled cartridge includes a cylinder portion, an upper lid in which a through hole for a liquid to be treated is formed and which is provided on an upper end of the cylinder portion, a lower lid in which a through hole for a treated liquid is formed and which is provided on a lower end of the cylinder portion, an insertion pipe connected to the lower lid, having an O-ring attached to an outer side, and inserted into a treated liquid discharge pipe provided on a bottom portion of the accommodating container of the metal removing column, and the ion exchanger filled inside the cylinder portion.
    Type: Grant
    Filed: November 10, 2017
    Date of Patent: April 19, 2022
    Assignee: ORGANO CORPORATION
    Inventors: Noriko Takada, Akira Nakamura
  • Patent number: 11291974
    Abstract: The present invention provides novel chromatographic materials, e.g., for chromatographic separations, processes for their preparation and separations devices containing the chromatographic materials. The preparation of the inorganic/organic hybrid materials of the invention wherein a surrounding material is condensed on a porous hybrid core material will allow for families of different hybrid packing materials to be prepared from a single core hybrid material. Differences in hydrophobicity, ion-exchange capacity, surface charge or silanol activity of the surrounding material may be used for unique chromatographic separations of small molecules, carbohydrates, antibodies, whole proteins, peptides, and/or DNA.
    Type: Grant
    Filed: June 1, 2010
    Date of Patent: April 5, 2022
    Assignee: Waters Technologies Corporation
    Inventor: Kevin D. Wyndham
  • Patent number: 11285473
    Abstract: Sample preparation systems and methods are described having pump control, valve configurations, and control logic that facilitate automatic, inline preparation dilutions of a sample according to at least two dilution operating modes. A system embodiment includes, but is not limited to a first pump configured to drive a carrier fluid; a second pump configured to drive a diluent; and a plurality of selection valves fluidically coupled with the first pump and the second pump, the plurality of selection valves being configured to direct fluid flows from the first pump and the second pump according to at least two modes of operation to provide a single-stage sample dilution according to a first operating mode and to provide a dual-stage sample dilution according to a second operating mode.
    Type: Grant
    Filed: November 10, 2020
    Date of Patent: March 29, 2022
    Assignee: ELEMENTAL SCIENTIFIC, INC.
    Inventors: Daniel R. Wiederin, Austin Schultz
  • Patent number: 11285403
    Abstract: The disclosure relates to a recycling chromatography method that includes injecting a sample into a mobile phase flow stream of a chromatography system to create a combined flow stream. The sample includes an API and at least one impurity. The chromatography system includes a first column and a column in series, a first valve in fluid communication with the first and second chromatographic columns, a heater in communication with the first and second chromatographic columns, a fraction collector in fluid communication with the first and second chromatographic columns, and a second valve positioned before the fraction collector. The combined flow stream is recycled from the first chromatographic column to the second chromatographic column and vice versa by switching the first valve until a baseline resolution is achieved to separate the at least one impurity from the API. The at least one impurity is collected in the fraction collector.
    Type: Grant
    Filed: May 30, 2019
    Date of Patent: March 29, 2022
    Assignee: Waters Technologies Corporation
    Inventors: Fabrice Gritti, Sylvain Cormier
  • Patent number: 11285404
    Abstract: An object of the present invention is to provide a packing material suitable for use as a packing material for size exclusion chromatography for fractionation that requires large-scale treatment, the packing material being capable of being produced by a simple process and reducing column pressure drop even when the particle diameter is small, and is to provide a method for producing the packing material. In the present invention, a packing material for size exclusion chromatography is obtained by a production process including polymerizing glycerol 1,3-dimethacrylate and glycidyl methacrylate in the presence of a polymerization initiator, hydrophilizing the resulting porous particles made of a copolymer using a sugar alcohol, and then opening the rings of remaining glycidyl groups using a mineral acid.
    Type: Grant
    Filed: February 9, 2018
    Date of Patent: March 29, 2022
    Assignee: SHOWA DENKO K.K.
    Inventor: Shuhei Maruta
  • Patent number: 11262224
    Abstract: Methods and apparatuses associated with an example flow sensing device are provided. In some examples, the flow sensing device may include a flow cap component and a sensor component. In some examples, the flow cap component may include a heating element disposed in a first layer of the flow cap component. In some examples, the sensor component may include at least one thermal sensing element disposed in a second layer of the sensor component. In some examples, the first layer and the second layer are noncoplanar. In some examples, the flow cap component may be bonded to a first surface of the sensor component to form a flow channel. In some examples, the first layer and the second layer may be noncoplanar and separated by the flow channel.
    Type: Grant
    Filed: June 19, 2020
    Date of Patent: March 1, 2022
    Assignee: HONEYWELL INTERNATIONAL INC.
    Inventors: Scott Edward Beck, Yong-Fa Wang, Philip C. Foster
  • Patent number: 11247192
    Abstract: Provided is a packing material for HILIC columns for more accurately and more easily performing oligosaccharide analysis by liquid chromatography; an HILIC column which is filled with the packing material for HILIC columns; and a method for analyzing an oligosaccharide with use of this packing material for HILIC columns A packing material for HILIC columns according to the present invention is composed of particles, each of which is obtained by reacting glycidol to a hydroxyl group of a porous cross-linked polymer base material having the hydroxyl group, and which have a hydrophilicity index of 2.30 or more and a surface-pH index of from 0.95 to 1.05.
    Type: Grant
    Filed: August 10, 2017
    Date of Patent: February 15, 2022
    Assignee: SHOWA DENKO K.K.
    Inventors: Junya Kato, Hideyuki Kondo, Ryuji Takahashi
  • Patent number: 11241638
    Abstract: Herein is reported the use of a chromatography column support comprising at least one plane of symmetry, one axis of symmetry, at least three legs, at least three straight connectors, whereby the connectors define a plane that is perpendicular to the axis of symmetry of the support, whereby the connectors are connected to each other at the axis of symmetry, whereby each leg is connected to a connector, whereby each leg is perpendicular to the plane defined by the connectors, whereby all legs are on the same side of the plane defined by the connectors for stabilizing the packing of a chromatography column.
    Type: Grant
    Filed: April 10, 2019
    Date of Patent: February 8, 2022
    Assignee: Hoffmann-La Roche Inc.
    Inventor: Andreas Blaschyk
  • Patent number: 11241640
    Abstract: A solvent reservoir filter for a liquid chromatograph system includes a first screen extending in a first plane, the first screen configured to filter solvent received through the first screen, a second screen extending in a second plane that is parallel to the first plane, the second screen configured to filter solvent received through the second screen, a main body extending between and connecting the first screen and the second screen, and a fluid outlet configured to expel solvent filtered by the first and second screens from the solvent reservoir filter. Methods of use and assembly of the solvent reservoir filter for a liquid chromatograph system are further disclosed.
    Type: Grant
    Filed: June 24, 2019
    Date of Patent: February 8, 2022
    Assignee: WATERS TECHNOLOGIES CORPORATION
    Inventors: Mark W. Moeller, William B. Clark
  • Patent number: 11224827
    Abstract: Disclosed is a chromatography column cylinder (200) formed from a polymer composite comprising an outer layer (220) and inner layer (210) intended to be in direct contact with the intended contents of the cylinder in a volume (202), wherein the outer layer supports the inner layer, for example when there is negative pressure in the volume (202). In one embodiment, one of said layers is molded directly onto a surface of the other layer such that there is no gap between the two layers. Disclosed also is a method for forming a chromatography column cylinder.
    Type: Grant
    Filed: November 10, 2017
    Date of Patent: January 18, 2022
    Assignee: Cytiva Sweden AB
    Inventors: Mats Lundkvist, Daniel Salomonsson
  • Patent number: 11221315
    Abstract: A structural reinforcement and biocompatible pump head for a pump includes a reinforcement structure having a plurality of ports and fluid pathways therein. The fluid pathways in the reinforcement may be coated or lined with a biocompatible material to form a biocompatible pump head useful for liquid chromatography and other analytical instrument systems. The biocompatible material may be injection molded into the fluid pathways of the reinforcement structure and may be machined after core pins are removed to obtain a desired surface finish and/or size of the biocompatible fluid pathways of the pump head.
    Type: Grant
    Filed: April 6, 2018
    Date of Patent: January 11, 2022
    Assignee: IDEX HEALTH & SCIENCE LLC
    Inventors: David Medeiros, Scott Ellis, Eric Beemer, Jason Polly
  • Patent number: 11207631
    Abstract: A filter assembly includes a conditioning device that conditions a flow of air upstream of a mass air flow sensor. The filter assembly includes a support frame. The filter assembly further includes a filter media coupled to the support frame, the filter media having a dirty side configured to receive a stream of air and a clean side configured to output a stream of air that has been filtered through the filter media. The filter assembly includes a conditioning device coupled to the support frame, the conditioning device positioned in a downstream direction from the clean side of the filter media with respect to the stream of air, the conditioning device offset from the clean side of the filter media by a separation distance.
    Type: Grant
    Filed: August 27, 2019
    Date of Patent: December 28, 2021
    Assignee: CUMMINS FILTRATION IP, INC.
    Inventors: Miao Li, Peter K. Herman, John C. Lukasavitz, Abhijit Shimpi, Gregory W. Hoverson, Thomas J. Braun, Robert A. Bannister
  • Patent number: 11207659
    Abstract: A carrier for adsorption a compound, comprising a support; and a shrink-fitted monolithic body attached to and surrounding at least a portion of the support. The monolithic body can be porous and configured to bind compounds in a solution either for the isolation or depletion of the compounds from the solution.
    Type: Grant
    Filed: October 19, 2018
    Date of Patent: December 28, 2021
    Assignee: DIONEX CORPORATION
    Inventors: Christopher A. Pohl, John M. Riviello
  • Patent number: 11198904
    Abstract: A substrate for medical test and a gene sequencing method thereof are disclosed. The substrate for medical test includes a micro flow channel substrate, a first substrate, and a second substrate. A side of the micro flow channel substrate facing the first substrate is provided with at least one first micro flow channel, and the first substrate includes a first sample inlet and a first sample outlet which are in communication with the first micro flow channel; a side of the micro flow channel substrate facing the second substrate is provided with at least one second micro flow channel, and the second substrate includes a second sample inlet and a second sample outlet which are communication with the second micro flow channel.
    Type: Grant
    Filed: January 26, 2018
    Date of Patent: December 14, 2021
    Assignees: BEIJING BOE OPTOELECTRONICS TECHNOLOGY CO., LTD., BOE TECHNOLOGY GROUP CO., LTD.
    Inventors: Peizhi Cai, Fengchun Pang, Yue Geng, Chuncheng Che, Hailin Xue
  • Patent number: 11187700
    Abstract: A closed system is provided for enlarging viral and bacterial particles for identification by diffraction scanning. The closed system includes a transparent tube, a deformable dispenser, a quantity of first antibodies, a quantity of second antibodies, and a diffraction scanning device. The transparent tube contains the quantity of first antibodies, the quantity of second antibodies, and receives the exhaled air from an individual. The deformable dispenser drives the quantity of second antibodies to mix with a complex formed by target matter in the exhaled air and the quantity of first antibodies. The quantity of first antibodies interacts with the target matter. The quantity of second antibodies interacts with the complex formed by the target matter and the quantity of first antibodies. The diffraction scanning device measures the number and size of particles within the transparent tube.
    Type: Grant
    Filed: January 28, 2021
    Date of Patent: November 30, 2021
    Inventor: Eckhard Kemmann
  • Patent number: 11175266
    Abstract: A method of preparing a liquid mixture for use in a liquid chromatography system is provided. The mixture comprises one or more acids, one or more bases, one or more solvents and water, and the method comprises the steps of: calculating pH and/or solvent concentration at a particular time t from a user-determined gradient function; and, based on the values obtained, calculating percent acid, percent base, percent solvent and percent water in the liquid mixture at time t. A liquid chromatography system incorporating such method is also provided.
    Type: Grant
    Filed: August 14, 2019
    Date of Patent: November 16, 2021
    Assignee: Waters Technologies Corporation
    Inventors: Thomas E. Wheat, Patricia McConville, John R. Lamoureux, Aparna Chavali
  • Patent number: 11173418
    Abstract: In various aspects, the present disclosure pertains to materials (e.g., kits, column assemblies, liquid chromatography systems, etc.) methods for performing liquid chromatography that employ a first column (e.g., a trapping column) and a second column (e.g., an analytical column). The first column comprises a first chromatographic material having a first chromatographic surface that comprises first hydrophobic surface groups and first ionizable surface groups having a first pKa value. The second column comprises a second chromatographic material having a second chromatographic surface that comprises second hydrophobic surface groups and (a) permanently ionized surface groups or (b) second ionizable surface groups having a second pKa value. The first hydrophobic surface groups have a hydrophobicity that is less than a hydrophobicity of the second hydrophobic surface groups.
    Type: Grant
    Filed: July 9, 2019
    Date of Patent: November 16, 2021
    Assignee: Waters Technologies Corporation
    Inventors: Matthew A. Lauber, Babajide O. Okandeji
  • Patent number: 11162922
    Abstract: Before start of analysis, a user inputs pipe capacity difference relative to reference pipe capacity and separation conditions, such as a mobile phase flow rate. A control unit calculates retention time shift from the pipe capacity difference and the flow rate. The control unit controls sample injection and data processing units so as to start collection of chromatogram data when a correction time has passed from the time point of sample injection in the case in which the value of the retention time shift is a positive value, and to perform sample injection when a correction time has passed from the time point at which the collection of chromatogram data is started in the case in which the value of the retention time shift is a negative value. Retention time shift caused by difference in the pipe capacity is corrected even when the mobile phase flow rate is different.
    Type: Grant
    Filed: August 3, 2018
    Date of Patent: November 2, 2021
    Assignee: SHIMADZU CORPORATION
    Inventor: Kohji Okada
  • Patent number: 11161057
    Abstract: Disclosed is a device for a solid phase extraction comprising two or more of the sorbents to remove phospholipids and salts from a sample, to thereby eliminate matrix effects during mass spectrometry analysis. In particular, the sorbents includes at least one sorbent which is water-wettable and contains at least one hydrophobic component and at least one hydrophilic component and at least one of sorbent having a specific affinity for a matrix interference like phospholipids. Further disclosed is a method using the device of the present invention.
    Type: Grant
    Filed: December 16, 2015
    Date of Patent: November 2, 2021
    Assignee: Waters Technologies Corporation
    Inventors: Pamela C. Iraneta, Xin Zhang, Frank John Marszalkowski, Jr.
  • Patent number: 11152200
    Abstract: An interface device, for providing a fluidic interface between a sample separation device and a mass spectrometer, includes an emitter capillary and a plurality of sample capillaries. The sample capillaries are movably arranged within the emitter capillary for transferring fluidic sample from the sample separation device to the mass spectrometer.
    Type: Grant
    Filed: January 30, 2020
    Date of Patent: October 19, 2021
    Assignee: Agilent Technologies, Inc.
    Inventor: Hans-Peter Zimmermann
  • Patent number: 11143639
    Abstract: A system and method for separating the functions of a liquid chromatography (LC) system into physically separate systems that allow for a more versatile LC system, wherein an LC device provides column 50 a liquid solvent, a sample, and a pump and injector that pushes the sample in the solvent to an output port, and providing an attachable module containing a module input port, a column, a heater for the column, and at least one detector for on-column detection, then attaching the module to the LC device using a press-fit connection that enables the sample in the solvent to be pumped through the column to the at least one detector in order to separate, identify and quantify substances in the sample and transmit results from the at least one detector to the LC system for collection and analysis.
    Type: Grant
    Filed: February 26, 2019
    Date of Patent: October 12, 2021
    Assignee: BRIGHAM YOUNG UNIVERSITY
    Inventors: Xiaofeng Xie, Luke T. Tolley, Paul B. Farnsworth, H. Dennis Tolley, Milton L. Lee
  • Patent number: 11143636
    Abstract: A fluid distributor unit comprising a channel system where one or more inlet channels (2) starting on an inlet side (3) of the distributor unit branch out successively into several channels (6) ending on the other side of the distributor unit, called the outlet side (4), characterised in that said distributor unit is provided in one single body (1) by free form fabrication.
    Type: Grant
    Filed: May 24, 2010
    Date of Patent: October 12, 2021
    Assignee: Cytiva Sweden AB
    Inventors: Klaus Gebauer, Jan Kranse, Mats Lundkvist
  • Patent number: 11130125
    Abstract: A method for manufacturing a fluidic device is provided. The method comprises providing a capillary, providing a structure having a fluidic channel and an opening, reducing an outer diameter of a portion of the capillary to be smaller than the opening of the structure. Furthermore, the method comprises inserting, at least partly, the portion of the capillary through the opening of the structure into the fluidic channel and applying heat to the structure to expand the inserted portion of the capillary to fit the capillary to the structure.
    Type: Grant
    Filed: August 6, 2019
    Date of Patent: September 28, 2021
    Assignee: Bio-Rad Laboratories, Inc.
    Inventors: Govind Kaigala, Robert Dean Lovchik, Anna Fomitcheva Khartchenko, Iago Pereiro Pereiro, Lorenzo Franco Teodoro Petrini
  • Patent number: 11125730
    Abstract: A flow passage unit has a column for used in a liquid chromatograph and a support body that supports the column. The column has: a porous stationary phase; a porous pressure adjusting part disposed at least at the flow-in end of the stationary phase, a liquid entering the flow-in end, the pressure adjusting part being harder than the stationary phase; and a covering part that covers the stationary phase and pressure adjusting part. The support body has a first plate and a second plate that are mutually joined, the support body forming a column holding part and a liquid flow passage, the column holding part holding the column between the first plate and the second plate, the liquid flow passage communicating with the column holding part. Pressure applied from the first plate and second plate to the pressure adjusting part is higher than pressure applied to the stationary phase.
    Type: Grant
    Filed: December 7, 2017
    Date of Patent: September 21, 2021
    Assignee: ALPS ALPINE CO., LTD.
    Inventors: Yoshimune Suzuki, Setsuo Ishibashi, Yoshihiro Taguchi, Satoshi Kawamura, Junko Ito, Hiroyoshi Minaguchi
  • Patent number: 11123656
    Abstract: The present invention relates to a method of separating and analyzing a mixture of oligonucleotides, including performing liquid chromatography using a column packed with a packing material obtained by fixing a diol to a surface of each of porous particles formed of a crosslinked organic polymer. According to this method, the oligonucleotides can be separated and analyzed with higher sensitivity compared to cases where columns having silica gel as a base material are used. In addition, the column can be washed with an alkaline solution.
    Type: Grant
    Filed: June 21, 2018
    Date of Patent: September 21, 2021
    Assignee: SHOWA DENKO K.K.
    Inventors: Junji Sasuga, Hirobumi Aoki, Yuzuru Kokido
  • Patent number: 11112344
    Abstract: According to one embodiment, a particle measuring method is disclosed. The method includes irradiating a detection liquid with light. The detection liquid contains methyl salicylate. The method further includes converting scattered light from the detection liquid into an electric signal by using photoelectric conversion after irradiating the detection liquid with the light. The method further includes performing a particle measurement on the detection liquid by using the electric signal.
    Type: Grant
    Filed: March 3, 2020
    Date of Patent: September 7, 2021
    Assignees: Kioxia Corporation, Rion Co., Ltd.
    Inventors: Hiroshi Tomita, Hidekazu Hayashi, Eishi Shiobara, Kaoru Kondo, Takuya Tabuchi, Kazuna Bando, Sota Kondo
  • Patent number: 11110375
    Abstract: Microstructure separation filters are provided herein, as well as chromatography and other separation devices. An exemplary filter device includes a microstructure filter has a plurality of layers of alternating sacrificial and/or structural material which have been etched to create inlet channels and outlet channels. Adjacent ones of the inlet channels and the outlet channels are spaced apart from one another by cross channels that filter a fluid from the inlet channels to the outlet channels. The cross channels include filter features formed by etching away of a portion of the layers. The device also includes a housing configured to receive the microstructure filter.
    Type: Grant
    Filed: October 16, 2018
    Date of Patent: September 7, 2021
    Assignee: Imagine TF, LLC
    Inventor: Brian Edward Richardson
  • Patent number: 11110458
    Abstract: System, including methods and apparatus, for spacing droplets from each other and for detection of spaced droplets.
    Type: Grant
    Filed: February 3, 2014
    Date of Patent: September 7, 2021
    Assignee: Bio-Rad Laboratories, Inc.
    Inventors: George Carman, Thomas H. Cauley, III, David P. Stumbo
  • Patent number: 11097208
    Abstract: A vise for column, for attaching a plug to a column tube of a column for chromatography comprising the column tube and the plug to be attached to one end of the column tube, is provided. The vise comprises a column supporter configured to be able to support a part of the column for chromatography, a main body configured to be connected to the column supporter and extending from the column supporter by a predetermined distance, and a column presser configured to be supported at another end of the main body opposite to one end to which the column supporter is connected, and configured to be movable toward and away from the column supporter, and configured to be able to press the plug against the column tube.
    Type: Grant
    Filed: March 6, 2019
    Date of Patent: August 24, 2021
    Assignee: JNC CORPORATION
    Inventors: Naoki Yamanaka, Masami Todokoro
  • Patent number: 11090631
    Abstract: The present invention provides a method for hydrophobization of a hydrophilic material, the method including introducing a hydrophobic group into a hydroxyl group (—OH group) on a surface of the hydrophilic material. A method for hydrophobization of a hydrophilic material, the method comprising reacting a hydrophilic material to be hydrophobized with a hydrophobic group-containing silylating agent in presence of an amino acid as a reaction accelerator, to introduce a hydrophobic group-containing silyl group to a surface of the hydrophilic material. A hydrophobized silica gel column filler is produced by using the method. Further, a hydrophobized silica gel column is produced by filling a column with the hydrophobized silica gel column filler.
    Type: Grant
    Filed: March 6, 2020
    Date of Patent: August 17, 2021
    Assignee: SHIMADZU CORPORATION
    Inventors: Yukio Oikawa, Shigeyoshi Horiike, Hirotaka Koyama
  • Patent number: 11092574
    Abstract: The invention relates to poly-amide bonded hydrophilic interaction chromatography (HILIC) stationary phases and novel HILIC methods for use in the characterization of large biological molecules modified with polar groups, known to those skilled in the art as glycans. The invention particularly provides novel, poly-amide bonded materials designed for efficient separation of large biomolecules, e.g. materials having a large percentage of larger pores (i.e. wide pores). Furthermore, the invention advantageously provides novel HILIC methods that can be used in combination with the stationary phase materials described herein to effectively separate protein and peptide glycoforms by eliminating previously unsolved problems, such as on-column aggregation of protein samples, low sensitivity of chromatographic detection of the glycan moieties, and low resolution of peaks due to restricted pore diffusion and long intra/inter-particle diffusion distances.
    Type: Grant
    Filed: April 2, 2015
    Date of Patent: August 17, 2021
    Assignee: Waters Technologies Corporation
    Inventors: Matthew A. Lauber, Stephan M. Koza, Pamela C. Iraneta, Kevin D. Wyndham
  • Patent number: 11079360
    Abstract: Methods of chromatographic detection are provided that include the step of providing a chromatography instrument without an autosampler, an external device, a pressurized sample injection device and a chromatographic data system. The chromatographic instrument has a sample valve, a column manager and a solvent manager. The present methods further include the steps: injecting sample into the sample valve with a pressurized sample injection device; transmitting a signal from the chromatographic data system to the external device, receiving the signal by the external device, producing an event output with the external device, discharging solvent from the solvent manager into the sample valve, and discharging sample into the column for chromatographic detection.
    Type: Grant
    Filed: September 24, 2018
    Date of Patent: August 3, 2021
    Assignee: WATERS TECHNOLOGIES CORPORATION
    Inventors: Aaron D. Phoebe, Sylvain G. Cormier, Charles H. Phoebe, Jr.
  • Patent number: 11058970
    Abstract: A vise for column, for attaching a plug to a column tube of a column for chromatography comprising the column tube and the plug to be attached to one end of the column tube, is provided. The vise comprises a column supporter configured to be able to support a part of the column for chromatography, a main body configured to be connected to the column supporter and extending from the column supporter by a predetermined distance, and a column presser configured to be supported at another end of the main body opposite to one end to which the column supporter is connected, and configured to be movable toward and away from the column supporter, and configured to be able to press the plug against the column tube.
    Type: Grant
    Filed: March 6, 2019
    Date of Patent: July 13, 2021
    Assignee: JNC CORPORATION
    Inventors: Naoki Yamanaka, Masami Todokoro