Chromatography Patents (Class 210/198.2)
  • Patent number: 10288635
    Abstract: Provided is a sample analyzing apparatus with which multiple analyses can be performed at the same time in a rapid and accurate manner using a small quantity of a liquid to be measured. This biochemical analyzing apparatus (50) is provided with a chip holder (53) into which an analysis chip (10) can be installed, a chip holder rotation unit (54) for rotating the chip holder (53), a pipetting unit (90) for injecting a candidate liquid into injection ports (22) in the analysis chip (10), and a measurement unit (80) capable of collectively measuring the respective reactions of the candidate liquid and multiple types of antigens (30). The chip holder (53) is rotated by the chip holder rotation unit (54), and injection of the candidate liquid is performed by the pipetting unit (90).
    Type: Grant
    Filed: May 12, 2015
    Date of Patent: May 14, 2019
    Assignee: Takano Co., Ltd.
    Inventors: Tamotsu Minegishi, Shinya Yamamoto, Takamitsu Godo, Kazutaka Hori
  • Patent number: 10290756
    Abstract: Provided are a novel multilayer structure that can be used to protect a device and a device using the multilayer structure. The disclosed multilayer structure is a multilayer structure including a substrate and a barrier layer stacked on the substrate. The 3% strain tension of the substrate in at least one direction is at least 2000 N/m. The barrier layer contains a reaction product (R). The reaction product (R) is a reaction product formed by a reaction at least between a metal oxide (A) and a phosphorus compound (B). In an infrared absorption spectrum of the barrier layer in a range of 800 to 1400 cm?1, a wavenumber (n1) at which maximum infrared absorption occurs is in a range of 1080 to 1130 cm?1. A metal atom constituting the metal oxide (A) is essentially an aluminum atom.
    Type: Grant
    Filed: June 12, 2013
    Date of Patent: May 14, 2019
    Assignee: KURARAY CO., LTD.
    Inventors: Masakazu Nakaya, Noboru Higashida, Kentaro Yoshida, Ryoichi Sasaki, Manabu Shibata, Tatsuya Oshita
  • Patent number: 10283338
    Abstract: A mass spectrometer vacuum interface can include a skimmer apparatus having a skimmer aperture and an internal surface. A method of operating the mass spectrometer vacuum interface can include establishing an outwardly directed flow along the internal surface of the skimmer apparatus.
    Type: Grant
    Filed: July 17, 2017
    Date of Patent: May 7, 2019
    Assignee: Thermo Fisher Scientific (Bremen) GmbH
    Inventors: Alexander Alekseevich Makarov, Lothar Rottmann
  • Patent number: 10281441
    Abstract: A clamp for chromatography columns has a first seal with a first opening a movable seal with a second opening and a movable coupler. The movable coupler has first and second coupler seals with communicating third and fourth openings. The clamp is arranged for pressing a first chromatography column between the first seal and the first coupler seal and for pressing a second chromatography column between the movable seal and the second coupler seal, such that the first opening fluidly communicates with the second opening through the first chromatography column, the third and fourth openings and the second chromatography column.
    Type: Grant
    Filed: December 29, 2017
    Date of Patent: May 7, 2019
    Assignee: BIO-RAD LABORATORIES, INC.
    Inventor: David Lerner
  • Patent number: 10258979
    Abstract: The invention provides novel porous materials that are useful in chromatographic processes, e.g., solid phase extraction, and that provide a number of advantages. Such advantages include superior wetting characteristics, selective capture of analytes of interest, and non-retention of interfering analytes. In certain aspects, the materials feature at least one hydrophobic component, at least one hydrophilic component and a average pore diameter of about 100 ?to about 1000 ?. In certain embodiments the materials also exhibit a nitrogen content from about 1% N to about 20% N. In certain embodiments, the materials also exhibit more than 10% of the BJH surface area of the porous material is contributed by pores that have a diameter greater than or equal to 200 ?. The invention advantageously provides novel porous materials having a large percentage of larger pores (i.e. wide pores). The invention advantageously provides novel porous materials that overcome the problems of SPE of biological samples.
    Type: Grant
    Filed: May 18, 2012
    Date of Patent: April 16, 2019
    Assignee: WATERS TECHNOLOGIES CORPORATION
    Inventors: Darryl W. Brousmiche, Kevin D. Wyndham
  • Patent number: 10254203
    Abstract: The disclosure relates to a method for the selective separation of proteins from liquid biological materials which, based on the total quantity, contain a small proportion of one or more trace components, by means of the addition of polar organic solvents having a dipole moment in the range from 1.6 to 4.0 Debye, and adsorption of the proteins on a solid phase carrier, wherein the trace components are separated from the proteins bound to the solid phase carrier by adsorption by means of a) magnetic field, b) centrifugal force, c) gravitational force or d) compressive force, and the trace components remain in the liquid.
    Type: Grant
    Filed: March 16, 2015
    Date of Patent: April 9, 2019
    Assignee: Magtivio B.V.
    Inventors: Viorel Rusu, Sven Goethel
  • Patent number: 10254255
    Abstract: Exemplary embodiments are directed to a rotary selector valve that includes a valve body that includes a rotor and a stator. The stator includes a first port for flow of a first flow material and a second port for flow of a second flow material. The rotor includes a groove for flow of at least one of the first flow material and the second flow material. The rotor includes a vent groove disposed between the first port and the second port for venting at least a portion of at least one of the first flow material and the second flow material to an exterior of the valve body. Exemplary methods of operating a rotary selector valve and CO2-based chromatography flow systems including a rotary selector valve are also provided.
    Type: Grant
    Filed: September 18, 2013
    Date of Patent: April 9, 2019
    Assignee: WATERS TECHNOLOGIES CORPORATION
    Inventors: Robert A. Jencks, Mark W. Moeller
  • Patent number: 10254256
    Abstract: A method of packing a chromatography column, including: dispersing chromatographic media particles in a slurry solution to form a slurry, filling a chromatography column with the chromatographic media particles by introducing the slurry to the column and applying a pressure to pack the chromatographic media particles in the chromatography column. The slurry solution is preferably aqueous based slurry solution. The pressure is preferably applied and held substantially constant for a first period at a first pressure and is applied and held substantially constant for a second period, following the first period, at a second pressure higher than the first pressure.
    Type: Grant
    Filed: October 1, 2015
    Date of Patent: April 9, 2019
    Assignees: Thermo Hypersil-Keystone LLC, Dionex Corporation
    Inventors: Xiaodong Liu, Jennifer M. Nguyen, Ronald A. Sherant, Mark L. Tracy
  • Patent number: 10252185
    Abstract: An apparatus for reaction chromatography comprising: a chromatography column, the column having a fluid outlet for an eluate flow, wherein the fluid outlet is configured with two or more fluid ports, the two or more fluid ports comprising one or more reactant ports, wherein each reactant port is for connecting a reactant flow into the eluate flow to react with the eluate flow, and one or more product ports for receiving the reacted eluate flow; one or more reactant sources in fluid communication with the one or more reactant ports to provide the reactant flow; and one or more processing units in fluid communication with the one or more product ports to process the reacted eluate flow.
    Type: Grant
    Filed: May 17, 2013
    Date of Patent: April 9, 2019
    Assignees: Thermo Electron Manufacturing Limited, University of Western Sydney
    Inventors: Ross Andrew Shalliker, Michelle Camenzuli, Harald Ritchie
  • Patent number: 10254254
    Abstract: Provided is a preparative separation liquid chromatograph system and preparative separation condition searching method which allows for an easy setting of the preparative separation condition. A sample temporally separated into components by a separation column is introduced into a detector and a fraction collector, with each component fractionated and collected by the fraction collector based on the result of a detection by the detector. A controlling and processing unit holds the following data for each sample or compound in the form of a database: chromatogram data obtained when a liquid chromatograph analysis in a preparative separation condition searching mode is performed for various standard samples under a search condition; and chromatogram data obtained when a liquid chromatograph analysis in a preparative separation mode is performed under one or more sets of preparative separation conditions for the various standard samples, along with the preparative separation condition used in this analysis.
    Type: Grant
    Filed: August 7, 2015
    Date of Patent: April 9, 2019
    Assignee: SHIMADZU CORPORATION
    Inventors: Hidetoshi Terada, Yuji Katsuyama, Katsuhiko Miwa, Chie Kushibe
  • Patent number: 10241070
    Abstract: An electrolytic device includes four channels separated by three charged barriers. The device can be used to suppress an eluent stream containing separated sample analyte ions and/or to pretreat a sample stream containing unseparated analyte ions.
    Type: Grant
    Filed: April 23, 2018
    Date of Patent: March 26, 2019
    Assignee: DIONEX CORPORATION
    Inventors: Kannan Srinivasan, Sheetal Bhardwaj, Rong Lin
  • Patent number: 10232287
    Abstract: An apparatus for use in a liquid chromatography system includes a chromatography port and a tubing assembly having a chromatography tube coupled at one end to the chromatography port. The end of the tube has an end face covered with a corrosion-resistant material, for example, gold. The corrosion-resistant nature of the material protects the end of the tube from corrosion or erosion, which improves the quality and reliability of a seal between the end face of the tube and a sealing surface of the port. Alternatively, or in addition to covering the end face of the tube with the corrosion-resistant material, a gasket covered with or made of the corrosion-resistant material can be disposed between the end face of the tube and the port. This gasket extends the reach of the tube to facilitate bottoming out the tube within the port.
    Type: Grant
    Filed: March 4, 2013
    Date of Patent: March 19, 2019
    Assignee: WATERS TECHNOLOGIES CORPORATION
    Inventors: Kurt D. Joudrey, Steven D. Trudeau, Paul Keenan, Joseph A. Luongo, Daniel J. McCormick, Eugene Berthiaume
  • Patent number: 10227377
    Abstract: An arrangement for a column comprising a stand provided with a platform adapted to receive a second end of a tubular housing and for supporting the arrangement on a floor, a first end unit removably connected to a first end of the tubular housing, an adaptor assembly movable within said tubular housing and an adaptor rod connected to said adaptor assembly, which adaptor rod is arranged to extend through an opening in the first end unit. A frame is connected to the first end unit, which frame and first end unit can be lifted and lowered in a substantially vertical direction, so that the tubular housing may be removed and substituted for another tubular housing with different dimensions in relation to the removed tubular housing when the frame and first end unit are lifted.
    Type: Grant
    Filed: July 8, 2014
    Date of Patent: March 12, 2019
    Assignee: GE Healthcare Bio-Sciences AB
    Inventors: Johan Avallin, Per Denker, Carina Andersson
  • Patent number: 10221412
    Abstract: The present invention relates to a multiplex microfluidic device for selection of nucleic acid aptamers and a method for high-throughput selection of nucleic acid aptamers using the same, and more particularly to a multiplex microfluidic device (SELEX lap-on-a-chip) that uses an improved multiplex platform in place of the development of an aptamer for a single target and to a method for high throughput selection of aptamers using the same together with high-throughput sequencing. A multiplex microfluidic device according to the present invention can simultaneously detect aptamers for a plurality of targets, and it can greatly increase the screening throughput and greatly shorten the process time compared to conventional multiplex techniques.
    Type: Grant
    Filed: October 5, 2011
    Date of Patent: March 5, 2019
    Assignees: PCL, INC., DONGGUK UNIVERSITY INDUSTRY-ACADEMIC COOPERATION FOUNDATION
    Inventors: So Youn Kim, Ji-Young Ahn, Minjoung Jo, Tae Kyung Kim
  • Patent number: 10221209
    Abstract: The present invention provides a method and automated system for the purification of polypeptides including the direct filtration of solutions containing the polypeptides after purification.
    Type: Grant
    Filed: October 28, 2014
    Date of Patent: March 5, 2019
    Assignee: Lonza Biologics PLC
    Inventors: Mark R. Whickman, Sam Mansoor
  • Patent number: 10215741
    Abstract: Methods, systems, devices, and products for evaluating a fluid. Methods include introducing a sample comprising the fluid to a solvating fluid at a point in a chamber associated with the instrument at a first time to create a heterogeneous admixture; measuring concentrations of each of a plurality of components in the admixture at a plurality of distances from the point in the chamber at, at least one additional time later than the first time, each of the plurality of distances being non-zero; and estimating a relative concentration for each of the plurality of components in the fluid by extrapolating the relative concentration of each of the plurality of components in the sample at the point at the first time using the measured concentrations in the admixture at the plurality of distances.
    Type: Grant
    Filed: May 5, 2016
    Date of Patent: February 26, 2019
    Assignee: BAKER HUGHES, A GE COMPANY, LLC
    Inventor: Rocco DiFoggio
  • Patent number: 10201768
    Abstract: An adaptor for a chromatography column, the adaptor comprising a substantially circular bottom plate provided with an end surface; a ring shaped wall element arranged at the periphery of the bottom plate. The bottom plate and the wall element having a common axis and the adaptor is provided with a filter connection arrangement for removably connecting a filter on the end surface of the bottom plate of the adaptor.
    Type: Grant
    Filed: June 18, 2013
    Date of Patent: February 12, 2019
    Assignee: GE HEALTHCARE BIO-SCIENCES AB
    Inventors: Stefan Kjell Eriksson, Andreas Bergstrom
  • Patent number: 10197170
    Abstract: A rotary valve 1 comprising a stator 3 and a rotor 5, wherein the stator comprises a plurality of connection ports (17a-17l) and orifices (19a-19l) and the rotor comprises a plurality of pairs of rotor valve orifices (23a-23h) joined by transfer channels (25a-25d). The rotor is able to be placed in different working positions whereby fluids can be fed to, for example, chromatography columns and/or can be bypassed through or around the valve. The invention also relates to a system comprising two components such as chromatography columns and a rotary valve.
    Type: Grant
    Filed: December 10, 2015
    Date of Patent: February 5, 2019
    Assignee: GE HEALTHCARE BIO-SCIENCES AB
    Inventors: Bjorn Markus Olovsson, Christer Olof Eriksson
  • Patent number: 10196615
    Abstract: Disclosed herein is provided a virus purification and formulation process for purifying a flavivirus represented by one of a a Yellow Fever Virus, Japanese Encephalitis virus, Dengue virus, and West Nile virus. The highly purified flavivirus virus product is characterized as having a low level of sucrose without significant virus loss such as that which is typically encountered by prior art virus purification processes. The disclosed process captures and purifies the virus, separating it from the host cell proteins and DNA, and leaving the host cell proteins and DNA behind. The process also can be used to inactivate and/or concentrate the virus sufficiently for use in formulations.
    Type: Grant
    Filed: May 24, 2016
    Date of Patent: February 5, 2019
    Assignee: GE HEALTHCARE BIO-SCIENCES CORP.
    Inventors: John J. Vicalvi, Jr., Edward G. Hayman, Joseph Makowiecki
  • Patent number: 10189226
    Abstract: A three-dimensional micro-truss structure includes: a plurality of first struts extending along a first direction; a plurality of second struts extending along a second direction; a plurality of third struts extending along a third direction; and a plurality of fourth struts extending along a fourth direction, wherein the first, second, third, and fourth struts interpenetrate one another at a plurality of nodes and wherein at least one of the first, second, and third directions extends at a non-perpendicular angle with respect to a plane, the plane being substantially perpendicular to the fourth direction.
    Type: Grant
    Filed: January 15, 2014
    Date of Patent: January 29, 2019
    Assignee: HRL Laboratories, LLC
    Inventors: Joanna A. Kolodziejska, Alan J. Jacobsen
  • Patent number: 10188964
    Abstract: A method of making and loading a chromatography column includes selecting a column tube having an appropriate elasticity, and inner diameter and length, selecting appropriately sized flow distributors, a second flow distributor having a diameter that is larger than the inner diameter of the tube, permanently securing a first flow distributor to a first end of the tube, loading packing medium into the tube, inserting the second flow distributor into a second end of the tube to drive the second flow distributor into the tube to form a sealed chamber within the tube, adjusting the longitudinal position of the second flow distributor within the tube by applying a force to the second flow distributor until it reaches a desired location within the tube, and/or forcing liquid into the chamber to move the second flow distributor and permanently securing the properly positioned second flow distributor within the tube.
    Type: Grant
    Filed: January 30, 2013
    Date of Patent: January 29, 2019
    Assignee: REPLIGEN CORPORATION
    Inventors: Daniel P. Witt, William J. Wilde, Adrian Lowe, Michael Shamashkin, Peter Rezac, Alexander Slocum, Travis Ward
  • Patent number: 10189007
    Abstract: The objective of the present invention is to provide a method for easily and efficiently producing cellulose beads which have pore shape suitable for an adsorbent and of which adsorption performance is excellent without using highly toxic and highly corrosive auxiliary raw material and without industrially disadvantageous cumbersome step. The method for producing porous cellulose beads according to the present invention is characterized in comprising (a) the step of preparing a fine cellulose dispersion by mixing a low temperature alkaline aqueous solution and cellulose, (b) the step of preparing a mixed liquid by adding a crosslinking agent to the fine cellulose dispersion, (c) the step of preparing an emulsion by dispersing the mixed liquid in a dispersion medium, (d) the step of contacting the emulsion with a coagulating solvent.
    Type: Grant
    Filed: October 14, 2014
    Date of Patent: January 29, 2019
    Assignee: KANEKA CORPORATION
    Inventors: Asuka Hayashi, Yoshikazu Kawai, Ken-ichiro Morio
  • Patent number: 10183236
    Abstract: A chromatography column distribution system includes a set of first bed support ribs extending radially from an inner, first radial position near the center of the plate to an outer radial position nearer to the periphery of the plate and at least one set of intermediate bed support ribs starting at an intermediate radial position and extending to an outer radial position nearer to the periphery of the plate. Channels are formed between adjacent bed support ribs. The desired local effective channel height varies in accordance with a predetermined formula from the first radial position to the outer radial position. The transverse cross-sectional areas of the ribs or the channels are adapted such that the actual local effective channel height is within 15% of the desired local effective channel height over portions of the distribution system situated between the first radial position and the outer radial position.
    Type: Grant
    Filed: January 29, 2016
    Date of Patent: January 22, 2019
    Assignee: GE Healthcare Bio-Sciences AB
    Inventor: Klaus Gebauer
  • Patent number: 10183237
    Abstract: Disclosed is a method for equilibrating a chromatography column including the steps of (i) providing a mobile phase; and (ii) passing the mobile phase through a packed chromatography column; wherein the mobile phase includes two liquids, the proportions of which change as it is passed through the column. Also discussed are automated aspects of the method, as well as a system capable of performing such method.
    Type: Grant
    Filed: March 20, 2015
    Date of Patent: January 22, 2019
    Assignee: BIOTAGE AB
    Inventors: Sunil Rana, Steve Jordan, Geoff Davies
  • Patent number: 10184922
    Abstract: A capillary column includes a fused silica tubing and a polyimide coating over the fusing silica tubing. Additionally, the capillary column further includes a first plurality of integrated ferrules positioned along at least a first portion of the fused silica tubing and spaced apart from one another by a first fixed interval.
    Type: Grant
    Filed: March 29, 2016
    Date of Patent: January 22, 2019
    Assignee: Thermo Finnigan LLC
    Inventor: Edward B. McCauley
  • Patent number: 10181396
    Abstract: Methods, systems and devices that provide fluid devices with at least one SPE bed adjacent (upstream of) a separation channel which may be in communication with an inlet of a Mass Spectrometer. The fluid device can be configured to operate using independently applied pressures to a BGE reservoir and a sample reservoir for pressure-driven injection that can inject a discrete sample plug into a separation channel that does not require voltage applied to the sample reservoir and can allow for in-channel focusing methods to be used. The methods, systems and devices are particularly suitable for use with a mass spectrometer but optical or other electronic detectors may also be used with the fluidic devices.
    Type: Grant
    Filed: June 29, 2017
    Date of Patent: January 15, 2019
    Assignee: The University of North Carolina at Chapel Hill
    Inventors: John Scott Mellors, William A. Black, John Michael Ramsey
  • Patent number: 10174871
    Abstract: A tubing and ferrule assembly for use in joining a clad tubing to a fitting. The ferrule is bound to the clad tubing, which includes a partial jacket of stainless steel, such that the inert core protrudes from, and has a common outer diameter with, the jacket. The ferrule is bound to the jacket slightly more distant than necessary for the inert core to contact a fining. When the ferrule is retained in place, the inert core is compressed and provides a true zero dead volume connection between the clad tubing and the fitting. The relationship of the ferrule, the clad tubing, and the fitting limits the position of the clad tubing and therefore precludes damaging of the associated valve or component.
    Type: Grant
    Filed: October 20, 2016
    Date of Patent: January 8, 2019
    Assignee: Valco Instruments Company, L.P.
    Inventors: Stanley D. Stearns, H. Max Loy, Jr.
  • Patent number: 10155253
    Abstract: The invention discloses a method for individually assessing the cleanness of at least one of an upper (2) and a lower (3) bed support in a process column (1). The method comprises the steps of: a) providing a process column (1) comprising an upper (2) and a lower (3) bed support, a column chamber, a movable adaptor (5), an upper outlet (6) and a lower outlet (7); b) conveying liquid through one of the lower (3) and upper (2) bed supports, e.g. by moving the adaptor (5) downwards at a predetermined rate with one of the upper and lower outlets closed and the other open to force liquid through one of the lower and upper bed supports, and; c) measuring the differential pressure over the bed support through which liquid passes.
    Type: Grant
    Filed: November 25, 2015
    Date of Patent: December 18, 2018
    Assignee: GE Healthcare Bio-Sciences AB
    Inventors: Lars Rosengren, Magnus Asplund, Niklas Pettersson
  • Patent number: 10150098
    Abstract: The present invention provides novel chromatographic materials, e.g., for chromatographic separations, processes for its preparation and separations devices containing the chromatographic material; separations devices, chromatographic columns and kits comprising the same; and methods for the preparation thereof.
    Type: Grant
    Filed: August 4, 2010
    Date of Patent: December 11, 2018
    Assignee: WATERS TECHNOLOGIES CORPORATION
    Inventors: Kevin D. Wyndham, Pamela C. Iraneta, Thomas H. Walter
  • Patent number: 10151759
    Abstract: A method of purifying a glycan and/or a glycoconjugate comprising the steps of: (a) providing a stationary phase that comprises cotton; (b) applying a glycan and/or glycoconjugate-containing sample to the stationary phase; (c) washing the stationary phase with a first solvent; and (d) eluting the glycan and/or glycoconjugate from the stationary phase with a second solvent. A kit for purifying a glycan and/or glycoconjugate, the kit comprising: a stationary phase comprising cotton; and instructions for purifying a glycan and/or glycoconjugate according to the disclosed method.
    Type: Grant
    Filed: February 10, 2012
    Date of Patent: December 11, 2018
    Assignee: Hoffmann-La Roche Inc.
    Inventors: Manfred Wuhrer, Mahdi Hemayatkar, Maurice Henricus Johannes Selman
  • Patent number: 10132798
    Abstract: The present invention relates to a microcolumn device for selecting nucleic acid aptamers for single and multiple target molecules, as well as a method for making the microcolumn device. The present invention also relates to a system for selecting nucleic acid aptamers for single and multiple target molecules. The present invention further relates to methods of using the microcolumn device for selecting nucleic acid aptamers for multiple target molecules. Kits that include one or more microcolumn device and/or system of the present invention are also disclosed.
    Type: Grant
    Filed: May 13, 2013
    Date of Patent: November 20, 2018
    Assignee: CORNELL UNIVERSITY
    Inventors: Harold G. Craighead, David R. Latulippe, John T. Lis, Abdullah Ozer, Kylan Szeto
  • Patent number: 10130900
    Abstract: A multi-walled tube that is useful as an analytical column in which chemical mixtures can be separated into their individual components is described. In order to be acceptable as an analytical column, the inner surface of the multi-walled tube must support effective separation, but not react chemically with or contaminate the solvent or the analyte (sample to be separated). Grade 316 stainless steel is typically preferred for this purpose. Moreover, the inner diameter (ID) surface of the multi-walled column is preferably very smooth (less than 10 micro inch Ra) with no interruptions in the surface such as scratches, pits, or asperities. However, since the column is designed to be attached to chromatographic equipment using standard size connection features, the size of standard fittings define the outer diameter (OD) of the column.
    Type: Grant
    Filed: June 16, 2017
    Date of Patent: November 20, 2018
    Assignee: Bandera Acquisition, LLC
    Inventors: Mark W. Broadley, John K. Eckert
  • Patent number: 10124275
    Abstract: Microstructure separation filters are provided herein, as well as chromatography and other separation devices. An exemplary filter device includes a microstructure filter has a plurality of layers of alternating sacrificial and/or structural material which have been etched to create inlet channels and outlet channels. Adjacent ones of the inlet channels and the outlet channels are spaced apart from one another by cross channels that filter a fluid from the inlet channels to the outlet channels. The cross channels include filter features formed by etching away of a portion of the layers. The device also includes a housing configured to receive the microstructure filter.
    Type: Grant
    Filed: September 4, 2015
    Date of Patent: November 13, 2018
    Assignee: Imagine TF, LLC
    Inventor: Brian Edward Richardson
  • Patent number: 10119944
    Abstract: The invention relates to poly-amide bonded hydrophilic interaction chromatography (HILIC) stationary phases and novel HILIC methods for use in the characterization of large biological molecules modified with polar groups, known to those skilled in the art as glycans. The invention particularly provides novel, poly-amide bonded materials designed for efficient separation of large biomolecules, e.g. materials having a large percentage of larger pores (i.e. wide pores). Furthermore, the invention advantageously provides novel HILIC methods that can be used in combination with the stationary phase materials described herein to effectively separate protein and peptide glycoforms by eliminating previously unsolved problems, such as on-column aggregation of protein samples, low sensitivity of chromatographic detection of the glycan moieties, and low resolution of peaks due to restricted pore diffusion and long intra/inter-particle diffusion distances.
    Type: Grant
    Filed: December 19, 2014
    Date of Patent: November 6, 2018
    Assignee: WATERS TECHNOLOGIES CORPORATION
    Inventors: Matthew A. Lauber, Stephan M. Koza, Pamela C. Iraneta, Kevin D. Wyndham
  • Patent number: 10118171
    Abstract: An ion-exchange composition suitable for use in ion exchange chromatography, comprising neutral vinyl polymer support particles irreversibly bound to a dispersant having ionizable sites which are un-ionized at a neutral pH and which are ionized under highly acidic or highly basic conditions, and fine layering particles functionalized with ion-exchanging sites on the surfaces thereof. A portion of the ionizable sites are ionized and bound electrostatically to a portion of the fine layering particles ion-exchanging sites, producing a support particle-dispersant-fine layering particle complex.
    Type: Grant
    Filed: July 30, 2015
    Date of Patent: November 6, 2018
    Assignee: DIONEX CORPORATION
    Inventors: Laurence A. Woodruff, Christopher A. Pohl, Jeffrey S. Rohrer
  • Patent number: 10092893
    Abstract: The present invention provides novel chromatographic materials, e.g., for chromatographic separations, processes for its preparation and separations devices containing the chromatographic material; separations devices, chromatographic columns and kits comprising the same; and methods for the preparation thereof. The chromatographic materials of the invention are chromatographic materials comprising having a narrow particle size distribution.
    Type: Grant
    Filed: July 25, 2011
    Date of Patent: October 9, 2018
    Assignee: WATERS TECHNOLOGIES CORPORATION
    Inventors: Kevin D. Wyndham, Beatrice W. Muriithi
  • Patent number: 10093815
    Abstract: A surface treatment agent capable of effectively preventing pattern collapse of an inorganic pattern or a resin pattern provided on a substrate, and a surface treatment method using such a surface treatment agent. The surface treatment agent includes a silylation agent and a nitrogen-containing heterocyclic compound which does not include a silicon atom.
    Type: Grant
    Filed: September 19, 2016
    Date of Patent: October 9, 2018
    Assignee: TOKYO OHKA KOGYO CO., LTD.
    Inventors: Daijiro Mori, Akira Kumazawa
  • Patent number: 10092858
    Abstract: An apparatus and a method are provided for column chromatography, which provide improvements in separation resolution and detection sensitivity, comprising a chromatography column having an inlet and an outlet, wherein the inlet is configured to introduce a flow of mobile phase into the column carrying a sample, wherein the inlet is further configured to introduce the flow of mobile phase into the column in at least two separate portions which are independently controllable, and to introduce the portions into different radial regions of the column, such that the portions flow longitudinally through the column in different radial regions. The sample preferably is contained in one of the portions, especially a central portion, in a higher concentration than in the other portion(s). Preferably the flow velocities of the portions are independently controllable.
    Type: Grant
    Filed: May 25, 2012
    Date of Patent: October 9, 2018
    Assignees: Thermo Electron Manufacturing Limited, University of Western Sidney
    Inventors: Harald Ritchie, Ross Andrew Shalliker
  • Patent number: 10092894
    Abstract: The present disclosure relates to a method of separating a compound of interest, particularly unsaturated compound(s) of interest, from a mixture. The compound is separated using a column having a chromatographic stationary phase material for various different modes of chromatography containing a first substituent and a second substituent. The first substituent minimizes compound retention variation over time under chromatographic conditions. The second substituent chromatographically and selectively retains the compound by incorporating one or more aromatic, polyaromatic, heterocyclic aromatic, or polyheterocyclic aromatic hydrocarbon groups, each group being optionally substituted with an aliphatic group.
    Type: Grant
    Filed: March 1, 2014
    Date of Patent: October 9, 2018
    Assignee: Waters Technologies Corporation
    Inventors: Darryl W. Brousmiche, Jacob N. Fairchild, Jason F. Hill, Giorgis Isaac, Michael F. Morris, Kevin D. Wyndham
  • Patent number: 10086128
    Abstract: The present invention provides devices and methods for concentrating a fluid and for treating a patient with the concentrated fluid. The concentrator apparatus includes a main housing (12) defining a separation chamber (14), a filter housing (48) containing a filter (46) comprising a filter element, a piping (44) for moving concentrated fluid from the separation chamber to the filter, and ports (32) for pressurizing the concentrated fluid past the filter element of the filter. The present invention also provides a variety of uses of concentrated body fluids, including autologous concentrated body fluid. The concentrated fluids can be used for example in surgical applications, including graft applications such as allograft, xenograft and autograft applications.
    Type: Grant
    Filed: July 2, 2014
    Date of Patent: October 2, 2018
    Assignee: SpineSmith Holdings, LLC
    Inventors: Matthew R. Kyle, Thomas Coull
  • Patent number: 10082489
    Abstract: Valves, pumps, detectors, sample loops, fraction collectors and the like are individually incorporated into modules that are mountable in interchangeable manner at individual mounting sites on a base unit which also supports one or more chromatography columns. Each module includes fluid connections to other modules and a microcontroller joining the module to a computed and monitor through an electronic connector at each mounting site. The fluid connections between the modules and the column(s) are removed from the electronic connections and accessible to the user. A software platform recognizes the modules and their locations, coordinates fluid connections between the modules, and provides a variety of control, monitoring, data generating and data processing functions to generate chromatographic data.
    Type: Grant
    Filed: March 15, 2016
    Date of Patent: September 25, 2018
    Assignee: Bio-Rad Laboratories, Inc.
    Inventors: Robert Iovanni, Alec Gordon, Bob Avarbock, Wayne Bland, Glenn Price, Ken Baker, Farah Mavandadi, Christof Schultz
  • Patent number: 10078071
    Abstract: Herein is described an apparatus for adaptation to existing GC/MS systems, utilizing a splitless injector as the desorption oven with a liquid CO2 cooled low thermal mass cryo trap that eliminates the need for flash heating of volatile compounds.
    Type: Grant
    Filed: September 29, 2015
    Date of Patent: September 18, 2018
    Assignee: The United States of America, as Represented by the Secretary of Agriculture
    Inventors: Hans T. Alborn, Steven D. Willms
  • Patent number: 10073037
    Abstract: The invention relates to the use of a construct for the detection of an analyte using surface enhanced spectroscopic techniques. The construct includes linked surfaces, where the link is formed by a linker providing a fixed inter-surface separation between the linked surfaces, wherein the linker is a linking compound, and at least one of the surfaces is the surface of a nanoparticle. The linking compound is suitable for interacting with the analyte. The linking compound may be a cucurbituril. The surface enhanced spectroscopic technique may be surface enhanced Raman spectroscopy (SERS).
    Type: Grant
    Filed: June 24, 2011
    Date of Patent: September 11, 2018
    Inventors: Richard William Taylor, Jeremy John Baumberg, Sumeet Mahajan, Tung-Chun Lee, Oren Alexander Scherman, Setu Kasera, Roger Coulston
  • Patent number: 10071361
    Abstract: A filter material is for the selective removal of siloxanes from a gas. The filter material contains a titanium compound, which is an organotitanate and/or a compound, which can be obtained by hydrolysis of an organotitanate. A method for the production of the filter material is also provided to use the filter material for the selective removal of siloxanes from a gas. A gas sensor is provided which includes the filter material.
    Type: Grant
    Filed: October 29, 2014
    Date of Patent: September 11, 2018
    Assignee: DRÄGER SAFETY AG & CO. KGAA
    Inventor: Jürgen Osswald
  • Patent number: 10073068
    Abstract: A liquid chromatography (LC) column includes a wall having a length along a central axis from the inlet end to the outlet end, the wall enclosing a column interior and having a column radius relative to the central axis, the wall comprising a structured portion configured such that the column radius varies along the length; and a plurality of particles packed in the column interior, wherein at least some of the particles are in contact with the structured portion.
    Type: Grant
    Filed: August 19, 2014
    Date of Patent: September 11, 2018
    Assignee: Agilent Technologies, Inc.
    Inventors: Hongfeng Yin, Reid A. Brennen, Eric Lyster, Roger Slocum
  • Patent number: 10067140
    Abstract: Embodiments of the present disclosure present novel systems, devices and methods for an automated biological sample analysis using mass-spectrometry. The time from sample introduction to the reporting of data, in some embodiments, takes a relatively short amount of time (e.g., several minutes). In some embodiments, a biological sample to be analyzed is a blood sample. For many applications, only a single drop of blood may be sufficient. Through the use of a mixture of standards with unique molecular mass, a quantitative analysis of the target analyte can be performed in a single MS run (for example), eliminating the need to create and analyze standard curves. One advantage of such embodiments may be that the system, devices, and methods can eliminate the need for batch creation since the requirement to amortize the time and effort of creating and analyzing standard curves can be eliminated.
    Type: Grant
    Filed: November 15, 2016
    Date of Patent: September 4, 2018
    Assignee: PureHoney Technologies, Inc.
    Inventor: Can Ozbal
  • Patent number: 10060560
    Abstract: Described is a mobile phase fitting having reduced corrosion and erosion. The fitting includes a coupling body, compression screw, compression member and gasket. The coupling body has a threaded bore at one end, a tapered cavity, a narrow bore and a fluid channel. The compression screw has an axial bore to receive a tube and a threaded outer surface in engagement with the threaded bore of the coupling body. The compression member is disposed in the tapered cavity and has an axial opening to pass the tube and a tapered surface to engage a surface of the tapered cavity. The gasket is disposed in the narrow bore and has one surface in contact with the first internal surface and has a parallel surface to receive an end face of the tube. Mobile phase flows along a path that includes the tube, an opening in the gasket and the fluid channel.
    Type: Grant
    Filed: June 25, 2013
    Date of Patent: August 28, 2018
    Assignee: WATERS TECHNOLOGIES CORPORATION
    Inventors: Jonathan L. Belanger, Sylvain Cormier, Steven D. Trudeau, Paul Keenan, Daniel J. McCormick
  • Patent number: 10060886
    Abstract: Described is a method of generating a flow having a composition gradient such as a mobile phase gradient for liquid chromatography. A pair of pumps is operated such that the initiations of pump strokes for one pump are controlled to occur between the initiations of pump strokes for the other pump so that the sequences of pump strokes fort the two pumps are interspersed in time. Initiations of the pump strokes of the second pump are offset in time relative to initiations of the pump strokes of the first pump such that variations in the flow rates of the first and second pumps due to initiation do not overlap in time. The volume of liquid contributed by a pump stroke is controlled according to the relative contribution of the respective pump to the composition gradient.
    Type: Grant
    Filed: February 3, 2015
    Date of Patent: August 28, 2018
    Assignee: WATERS TECHNOLOGIES CORPORATION
    Inventors: Michael R. Jackson, Christopher Seith, Joshua A. Shreve
  • Patent number: 10060885
    Abstract: Provided is a liquid chromatography apparatus for fast measuring, and more particularly, a liquid chromatography apparatus which includes a first column and a second column having different component separations for an interest component within a sample and makes components primarily separated in the first column be secondarily separated in the second column of a first channel unit before a switching valve is switched, makes the components primarily separated in the first column be continuously discharged through a second channel unit after the switching valve is switched, thereby improving the component separation and achieving the fast measuring.
    Type: Grant
    Filed: July 10, 2014
    Date of Patent: August 28, 2018
    Assignee: WITHTECH INC
    Inventors: Seoung-Kyo Yoo, Eung Sun Lee, Jin Yong Lee, Dae Hee Kim
  • Patent number: 10054961
    Abstract: The present invention provides manipulation of spacing between microdroplets flowing in a microfluidic system. The manipulation includes increasing and/or decreasing spacing between a pair of adjacent droplets of a sample fluid flowing in an immiscible carrier fluid within a main microfluidic channel. The main microfluidic channel includes a region having one or more side branch channels. The spacing between the pair of droplets flowing through the region can be increased or reduced with either the addition of or removal of immiscible carrier fluid between the adjacent droplets via at least one of the side branch channels, wherein the pair of droplets remain intact while flowing through the region and continue to flow within the main microfluidic channel downstream of the region.
    Type: Grant
    Filed: February 19, 2014
    Date of Patent: August 21, 2018
    Assignee: The University of Chicago
    Inventors: Rustem F. Ismagilov, Bo Zheng, Cory John Gerdts