Methods Patents (Class 250/459.1)
  • Patent number: 11513075
    Abstract: An observation device is an observation device observing an observation target and includes: an emission light source that generates emission light; a projection light source that generates projection light; a scanning mirror that scans the emission light and the projection light toward the observation target along the same optical path; a light guide optical system that guides detection target light generated in the observation target in accordance with emission of the emission light without it passing through the scanning mirror; an optical detector that detects the detection target light guided by the light guide optical system; and a control unit that controls an intensity of the projection light on the basis of a result of the detection of the detection target light.
    Type: Grant
    Filed: October 12, 2017
    Date of Patent: November 29, 2022
    Assignee: HAMAMATSU PHOTONICS K.K.
    Inventors: Akira Takahashi, Shunsuke Matsuda, Takayuki Miyashita, Mitsuharu Miwa
  • Patent number: 11506593
    Abstract: Methods for classifying fluorescent flow cytometer data are provided. In some instances, methods include processing the flow cytometer data with a supervised algorithm configured to cluster the fluorescent flow cytometer data into distinct populations according to the relationship of data points to relevant threshold values. In embodiments, methods include determining a measure of spillover spreading by calculating spillover spreading coefficients and combining them in a spillover spreading matrix. In some embodiments, populations of fluorescent flow cytometer data are adjusted to subtract the magnitude of spillover spreading. In embodiments, spillover spreading adjusted populations are partitioned after potential partitions are evaluated relative to the threshold values. In embodiments, partitioned populations of fluorescent flow cytometer data are classified (i.e., phenotyped) according to a hierarchy. Systems and computer-readable media for classifying fluorescent flow cytometer data are also provided.
    Type: Grant
    Filed: January 20, 2021
    Date of Patent: November 22, 2022
    Assignee: Becton, Dickinson and Company
    Inventor: Richard Lee Halpert
  • Patent number: 11454588
    Abstract: A fluorescent spectrum correcting method includes comparing fluorescent spectrum obtained from micro-particles labeled with a plurality of fluorescent pigments with reference spectrum to separating the fluorescent spectrum into fluorescent spectrum for each pigment, and previously measured spectrum data is used as the reference spectrum.
    Type: Grant
    Filed: January 6, 2021
    Date of Patent: September 27, 2022
    Assignee: SONY CORPORATION
    Inventors: Masaya Kakuta, Koji Futamura, Yoshitsugu Sakai, Yasunobu Kato
  • Patent number: 11454793
    Abstract: A microscope apparatus includes SLMs each having a modulation plane, an objective lens disposed on an optical path between the modulation plane and an object, and a computer for controlling the SLMs on the basis of a modulation pattern including a correction pattern for correcting aberration caused by a refractive index interface of the object. The computer determines a position of the correction pattern in the modulation pattern on the basis of inclination information of the refractive index interface with respect to a plane perpendicular to an optical axis of the objective lens.
    Type: Grant
    Filed: April 10, 2018
    Date of Patent: September 27, 2022
    Assignee: HAMAMATSU PHOTONICS K.K.
    Inventor: Naoya Matsumoto
  • Patent number: 11435228
    Abstract: Disclosed is a system and method for interrogating a photo-responsive material, such as for authentication purposes, utilizing a light source to illuminate a photo-responsive material, a detector to capture an emission from the photo-responsive material, and a processor to receive a response from the detector while the photo-responsive material is being illuminated after a maximum response has been received, then measuring a change in the received response.
    Type: Grant
    Filed: July 17, 2019
    Date of Patent: September 6, 2022
    Assignee: INTELLIGENT MATERIAL SOLUTIONS, INC.
    Inventors: Joshua E. Collins, Howard Y. Bell
  • Patent number: 11434082
    Abstract: A stuck slide determination system is provided that determines if a slide (e.g., glass slide), positioned on a scanning stage at the start of the scanning process, gets stuck and does not move along with the scanning stage after the scanning process commences. In an embodiment, the system includes a sensor having a transmitter and receiver that are relatively positioned to detect the presence of the stage or a slide or the absence of the stage or a slide. When the scanning stage begins to move at the start of the scanning process, a processor monitors a signal from the sensor and determines if the slide is improperly positioned after the scanning process has commenced. If the slide is improperly positioned, the processor stops movement of the scanning stage in order to protect the slide from damage.
    Type: Grant
    Filed: May 18, 2020
    Date of Patent: September 6, 2022
    Assignee: Leica Biosystems Imaging, Inc.
    Inventors: Nicholas Newberg, Prentash Djelosevic, Aaron Stearrett, Peyman Najmabadi
  • Patent number: 11434523
    Abstract: The present disclosure provides a system and method for the detection of rare mutations and copy number variations in cell free polynucleotides. Generally, the systems and methods comprise sample preparation, or the extraction and isolation of cell free polynucleotide sequences from a bodily fluid; subsequent sequencing of cell free polynucleotides by techniques known in the art; and application of bioinformatics tools to detect rare mutations and copy number variations as compared to a reference. The systems and methods also may contain a database or collection of different rare mutations or copy number variation profiles of different diseases, to be used as additional references in aiding detection of rare mutations, copy number variation profiling or general genetic profiling of a disease.
    Type: Grant
    Filed: June 26, 2020
    Date of Patent: September 6, 2022
    Assignee: GUARDANT HEALTH, INC.
    Inventors: AmirAli Talasaz, Stefanie Ann Ward Mortimer
  • Patent number: 11427137
    Abstract: An emblem arrangement for a motor vehicle has an emblem support part, an element forming a brand emblem, and at least one luminous element for illuminating the emblem arrangement. The element forming the brand emblem is a layered structure having at least two layers, which layered structure has, when viewed in a plan view of the emblem arrangement, translucent and non-translucent regions. The translucent regions are able to depict the shape of the brand emblem in its entirety or wherein the translucent regions are able to depict merely the contour of the brand emblem.
    Type: Grant
    Filed: December 2, 2019
    Date of Patent: August 30, 2022
    Assignee: Volkswagen Aktiengesellschaft
    Inventors: Christian Studeny, Falko Seifert, Miguel Almeida Estevao, Sandra Sturmat
  • Patent number: 11427742
    Abstract: A method of surface logging a well includes adding each of multiple polymeric taggants to a circulating drilling fluid in an addition sequence while drilling the well. Each polymeric taggant includes a polymer and a respective fluorescent dye having an emission wavelength or excitation wavelength different from that of each other fluorescent dye. The method includes taking a sample of drill cuttings carried by a drilling fluid while drilling a well, wherein the sample of drill cuttings includes polymeric taggants attached to the drill cuttings. The method includes extracting the dyes from the sample of drill cuttings into an extract solution; determining an indication of the type of and the concentration of each of the dyes in the extract solution; and determining a depth associated with the sample of drill cuttings based on the indication of the concentration of each of the dyes and on the addition sequence.
    Type: Grant
    Filed: August 24, 2020
    Date of Patent: August 30, 2022
    Assignee: Saudi Arabian Oil Company
    Inventors: Nouf AlJabri, Alberto F. Marsala, Shitong Sherry Zhu, Marta Antoniv
  • Patent number: 11421154
    Abstract: A composition for photoluminescence of the chemical formula M2(1-y-z)Yb2yRem2zADO5-xF2x, a method for producing said composition, and uses of said composition are provided.
    Type: Grant
    Filed: July 18, 2018
    Date of Patent: August 23, 2022
    Assignee: Karlsruher Institut für Technologie
    Inventors: Dmitry Busko, Guojun Gao, Ian Howard, Bryce Richards, Andrey Turshatov
  • Patent number: 11415528
    Abstract: A method and system for automated in-line inspection of optically transparent material is disclosed herein. The method includes illuminating a top and bottom surface of the optically transparent material with at least one sheet of light and then generating an image based on light that is received by an imaging device. The image that is generated may either be a bright field image or a dark field image.
    Type: Grant
    Filed: October 10, 2018
    Date of Patent: August 16, 2022
    Assignee: WDI WISE DEVICE INC.
    Inventors: Adam Weiss, Aleksey Lopatin
  • Patent number: 11389339
    Abstract: Techniques are disclosed for detecting a presence of a biological substance through an article such as a diaper. For example, a detection system causes a light source to transmit light through the article. The light includes a peak wavelength that corresponds to an excitation wavelength of a biological substance that may be present in the article. The detection system detects a measurement of light intensity within a range of emission wavelengths of the biological substance. By comparing the measurement of light intensity to a threshold, the detection system identifies a presence of the biological substance on the article.
    Type: Grant
    Filed: August 16, 2019
    Date of Patent: July 19, 2022
    Assignee: VERILY LIFE SCIENCES LLC
    Inventor: Brian Schiffer
  • Patent number: 11365349
    Abstract: The present invention relates to a method for producing luminescent nanoparticles wherein particle size of the nanoparticles is controlled. The method of the present invention includes admixing two or more rare earth metal salts in a first solvent and an organic oil to form a reaction mixture, and subjecting the reaction mixture to an inert gas so that flow rate of the inert gas is at least 2-5 L/h and pressure in the reaction vessel is at least 50 Pa over atmospheric pressure, preferably 50-80 Pa over atmospheric pressure.
    Type: Grant
    Filed: March 10, 2017
    Date of Patent: June 21, 2022
    Assignee: KAIVOGEN OY
    Inventors: Eeva Junkkari, Terhi Riuttamäki, Johanna Pyylampi, Jarkko Karvinen, Antti Pihlava
  • Patent number: 11363215
    Abstract: A light observation apparatus includes: a first photodetector that, upon irradiation of at least part of a physical object with irradiation light, receives first light containing ambient light and at least one selected from the group consisting of reflected light returning from the at least part and fluorescence produced from the at least part, and that outputs a first output signal representing a reception intensity of the first light; a second photodetector that receives second light containing the ambient light and outputs a second output signal representing a reception intensity of the second light; and a signal processing circuit. The signal processing circuit attenuates a first signal component corresponding to the ambient light from the first output signal based on the first output signal and the second output signal.
    Type: Grant
    Filed: February 7, 2020
    Date of Patent: June 14, 2022
    Assignee: PANASONIC INTELLECTUAL PROPERTY MANAGEMENT CO., LTD.
    Inventors: Tatsushi Ohyama, Mariko Miyashita
  • Patent number: 11342825
    Abstract: A rigid-flexible coupling high-accuracy galvo scanning motor comprises: a stator, a rotor rotating relative to the stator, bearing seats and at least two groups of encoders. The rigid-flexible coupling bearings are installed on the rotating shaft of the rotor; each of the rigid-flexible coupling bearings comprises: a rigid bearing and a flexible hinge ring which is elastically deformable, and the flexible hinge ring is fixed in an inner ring of the rigid bearing; the at least two groups of encoders comprise: a first group of encoders and a second group of encoders; the first group of encoders is used to measure a rotation angle of the rotating shaft; and the second group of encoders is used to measure a rotation angle of the inner ring of the rigid bearing. A friction dead zone is avoided through the elastic deformation of the flexible hinge ring, thereby reducing a disturbance bandwidth.
    Type: Grant
    Filed: January 14, 2020
    Date of Patent: May 24, 2022
    Assignee: Guangdong University of Technology
    Inventors: Zhijun Yang, Shengqiang Wei, Hao Peng, Youdun Bai, Guanxin Huang
  • Patent number: 11285477
    Abstract: A method for measuring a temperature of a fluid for use with a microfluidic analysis apparatus includes a reading step and a determination step. During the reading step, at least one diffusion signal is read, the latter representing a diffusion characteristic of the diffusion of the fluid. During the determination step, the temperature is determined using the at least one diffusion signal.
    Type: Grant
    Filed: August 29, 2019
    Date of Patent: March 29, 2022
    Assignee: Robert Bosch GmbH
    Inventor: Tino Frank
  • Patent number: 11268954
    Abstract: An immunoassay apparatus may include: a sample dispenser part that dispenses a sample into a first reaction container; a reagent dispenser part that dispenses, into the first reaction container: a solid-phase reagent containing a solid-phase carrier; a labeled reagent; and a releasing reagent that releases, from the solid-phase carrier, an immune complex including a target substance and a labeled substance; a measurement part that measures a signal based on the labeled substance in the immune complex in a second reaction container; a container supply part that stores a plurality of reaction containers; a transfer part that transfers the first reaction container so that the sample dispenser part and the reagent dispenser part perform a dispensing operation to the first reaction container, and that transfers the second reaction container so that the immune complex dispended from the first reaction container is dispensed into the second container.
    Type: Grant
    Filed: April 30, 2020
    Date of Patent: March 8, 2022
    Assignee: SYSMEX CORPORATION
    Inventors: Toshiyuki Sato, Toshikatsu Fukuju, Shuhei Kaneko, Atsushi Aoki, Tomohiro Okuzaki, Yasuhiro Takeuchi, Yutaka Kawamoto
  • Patent number: 11266756
    Abstract: A system for bacteria irradiation from an occupant zone of a vehicle is provided. The system comprises an interior portion disposed in the occupant zone. The interior portion has one of a predetermined surface porosity and a predetermined surface roughness. The interior portion has phosphorescent paint for visual indication. The system further comprises a high energy visible (HEV) light source integrated within the interior portion. The HEV light source has an emission wavelength of between about 375 nm and about 425 nm. The HEV light source further provides a cumulative energy of between about 1 J/cm2 and about 50 J/cm2. The system further comprises a vehicle processor in communication with the HEV light source. The vehicle processor is configured to enable the HEV light source. The system further comprises a sensor in communication with the vehicle processor and configured to provide input on when to enable light source.
    Type: Grant
    Filed: July 31, 2019
    Date of Patent: March 8, 2022
    Assignee: GM Global Technology Operations LLC
    Inventors: Janet C. Robincheck, Adam F. Gross, Nancy L. Johnson
  • Patent number: 11266771
    Abstract: To provide a concentration calculating module configured to measure the concentrations of two constituents simultaneously with higher accuracy. The concentration measuring module includes a light source configured to emit light into a housing; a first light receiving unit configured to have sensitivity to a wavelength of output light of the light source and receive light radiated from inside the housing; and a second light receiving unit configured to have sensitivity to a longer wavelength than the first light receiving unit and receive light radiated from inside the housing.
    Type: Grant
    Filed: July 13, 2018
    Date of Patent: March 8, 2022
    Assignee: ASAHI KASEI KABUSHIKI KAISHA
    Inventors: Sho Sugiyama, Shinji Miya, Yoshiro Yamaha
  • Patent number: 11262570
    Abstract: Methods for imaging a sample using fluorescence microscopy, systems for imaging a sample using fluorescence microscopy, and illumination systems for fluorescence microscopes. In some examples, a method includes positioning a dual convex paraboloidal mirror enclosure around the sample. The dual convex paraboloidal mirror enclosure includes an upper paraboloidal mirror and a lower paraboloidal mirror oriented antiparallel to each other. An aperture is defined in the lower paraboloidal mirror, a hemispherical dome is mounted in the aperture, and the sample is surrounded by the hemispherical dome. The method includes directing excitation light onto the sample to form a primary image at an upper vertex of the upper paraboloidal mirror and a secondary image at a lower vertex of the lower paraboloidal minor. The method includes imaging the sample through a detection objective of a microscope.
    Type: Grant
    Filed: March 12, 2019
    Date of Patent: March 1, 2022
    Assignee: THE UNIVERSITY OF NORTH CAROLINA AT CHAPEL HILL
    Inventors: Paul Samuel Maddox, Tanner Christian Fadero
  • Patent number: 11255791
    Abstract: A method of spatially measuring a plurality of nano-scale structures in a sample comprises the steps of: marking the individual structures at different locations with fluorescent markers, coupling the individual structures to individual positioning aids whose positions in the sample are known, exciting the fluorescent markers with excitation light for emission of fluorescence light, wherein an intensity distribution of the excitation light has a local minimum, arranging the local minimum at different positions in a close-up range around the position of respective positioning aid whose dimensions are not larger than the diffraction limit at the wavelength of the excitation light, registering the fluorescence light emitted out of the sample separately for the individual fluorescent markers and for the different positions of the minimum, and determining positions of the individual fluorescent markers in the sample from the intensities of the fluorescence light registered.
    Type: Grant
    Filed: August 28, 2020
    Date of Patent: February 22, 2022
    Assignee: MAX-PLANCK-GESELLSCHAFT ZUR FOERDERUNG DER WISSENSCHAFTEN
    Inventors: Stefan W. Hell, Yvan Eilers, Klaus Gwosch, Francisco Balzarotti
  • Patent number: 11239433
    Abstract: A compound comprising a ligand LA having the formula: is disclosed. Such a compound is suitable for use an emitter in organic light emitting devices.
    Type: Grant
    Filed: July 18, 2018
    Date of Patent: February 1, 2022
    Assignee: UNIVERSAL DISPLAY CORPORATION
    Inventors: Morgan C. MacInnis, Diana Drennan, Tongxiang Lu, Daniel W. Silverstein, Hsiao-Fan Chen, George Fitzgerald, Jason Brooks, Paul M. Lahti
  • Patent number: 11224681
    Abstract: To provide a concentration calculating module configured to measure the concentrations of two constituents simultaneously with higher accuracy. The concentration measuring module includes a light source configured to emit light into a housing; a first light receiving unit configured to have sensitivity to a wavelength of output light of the light source and receive light radiated from inside the housing; and a second light receiving unit configured to have sensitivity to a longer wavelength than the first light receiving unit and receive light radiated from inside the housing.
    Type: Grant
    Filed: July 13, 2018
    Date of Patent: January 18, 2022
    Assignee: ASAHI KASEI KABUSHIKI KAISHA
    Inventors: Sho Sugiyama, Shinji Miya, Yoshiro Yamaha
  • Patent number: 11221297
    Abstract: Accelerated methods and apparatuses for three-dimensional microscopy with structured illumination, in which focal planes of the sample are focused and each focal plane is illuminated in a plurality of phases sequentially with structured illumination light and the sample light emitted by the sample is recorded in a respective individual image. A resulting image having a resolution that is increased with respect to the individual images is reconstructed from the individual images to produce a super-resolved image stack, By reconstructing a resulting image from individual images of two different focal planes by approximation methods, said resulting image represents a sample plane that is situated between said focal planes, an image stack can be produced in a shorter period with less stress on the sample.
    Type: Grant
    Filed: November 7, 2019
    Date of Patent: January 11, 2022
    Assignee: Carl Zeiss Microscopy GmbH
    Inventors: Ingo Kleppe, Yauheni Novikau
  • Patent number: 11209237
    Abstract: A toy gun capable of illuminating soft projectile being ejected therefrom, comprising a gun body, a grip assembly is fixedly attached to the gun body, and a trigger assembly mounted on the grip assembly. The gun body has a power source, a motor, a launch assembly with a launch tube, a light source and a light reflecting unit. When the trigger assembly of the toy gun is actuated, the power source activates the motor and switches on the light source. The activated motor causes the launch assembly to discharge a soft projectile in the launch tube and the light emitted from the light source is reflected by the light reflecting unit onto the soft projectile once it is discharged, thereby illuminating the soft projectile.
    Type: Grant
    Filed: August 6, 2020
    Date of Patent: December 28, 2021
    Inventor: Siew Buan Quek
  • Patent number: 11209367
    Abstract: Methods and systems for fluorescence imaging are described herein. The method can include: receiving a fluorescence signal including an excitation signal, a first emission signal for a first fluorophore having a first emission spectra, and a second emission signal for a second fluorophore having a second emission spectra; filtering the fluorescence signal to: isolate a first channel encompassing at least one of: a bandwidth of at least 1 nm within which an emission intensity of the first emission spectra is at least twice an emission intensity of the second emission spectra, a bandwidth having a rising edge of the first emission spectra; and at least 10% by height of a rising edge of the first emission spectra, and produce a channel including the fluorescence signal less the first channel; and directing the first channel and the resulting channel to different regions of one or more cameras for collecting fluorescence emissions.
    Type: Grant
    Filed: August 26, 2019
    Date of Patent: December 28, 2021
    Assignee: YALE UNIVERSITY
    Inventors: Yongdeng Zhang, Joerg Bewersdorf
  • Patent number: 11199689
    Abstract: The invention relates to a method for microscopic evaluation (120) of a sample (2), in particular at least one uncolored object or cell sample (2), in an optical detection system (1), where the following steps are performed: providing at least two different detection information (110) about the sample (2), in particular by the detection system (1), performing an evaluation (120) of the detection information (110), in particular by an analysis means (60), on the basis of machine-learned transfer information (200), in order to determine result information (140) about the sample (2), the transfer information (200) being trained for a different detection parameterization of the detection information (110), in which the detection information (110) differs from one another in terms of at least one illumination parameter of the detection system (1), in particular in terms of polarization and/or color coding.
    Type: Grant
    Filed: July 19, 2019
    Date of Patent: December 14, 2021
    Assignee: Olympus Soft Imaging Solutions GmbH
    Inventors: Daniel Krueger, Mike Woerdemann, Stefan Diepenbrock
  • Patent number: 11195276
    Abstract: The invention provides systems and methods for imaging a sample. In various embodiments, the invention provides a system comprising an image sensor, a laser for emitting excitation light for an infrared or near-infrared fluorophore, a visible light source, a notch beam splitter, a notch filter, a synchronization module, an image processing unit, an image displaying unit, and light-conducting channels. In various embodiments, the present invention provides a system comprising an image sensor, a laser for emitting excitation light for an infrared or near-infrared fluorophore, a laser clean-up filter, a notch filter, a white light source, an image processing unit, an image displaying unit, and light-conducting channels. In accordance with the present invention, the image sensor can detect both visible light and infrared light.
    Type: Grant
    Filed: August 18, 2020
    Date of Patent: December 7, 2021
    Assignee: CEDARS-SINAI MEDICAL CENTER
    Inventors: Pramod Butte, Adam Mamelak
  • Patent number: 11194144
    Abstract: A microscopy method, and related microscope, including producing illumination radiation and directing it at a focus. The illumination radiation is switched temporally between at least two modes, such that focus modulation is effected at which temporally varying and mutually different mode fields of the illumination radiation are produced in the focus. The focus is guided at least over regions of a sample to be examined, wherein detection radiation in the sample is or may be brought about by the illumination radiation in the focus at least at a point of origin. The detection radiation is captured in a manner assigned to the at least one point of origin. In addition to the illumination radiation, at least one disexcitation beam of rays of disexcitation radiation is directed at the focus. The disexcitation radiation prevents the detection radiation from being brought about in the region that is illuminated by the disexcitation radiation.
    Type: Grant
    Filed: June 21, 2017
    Date of Patent: December 7, 2021
    Assignee: Carl Zeiss Microscopy GmbH
    Inventors: Ralf Netz, Thomas Kalkbrenner, Tiemo Anhut
  • Patent number: 11181490
    Abstract: An x-ray apparatus, that may include a mount that is configured to hold a sample; an x-ray source, that is configured to direct an x-ray beam toward a first side of the sample; a detector, positioned downstream to a second side of the sample, the detector is configured to detect, during a sample measurement period, at least a part of x-rays that have been transmitted through the sample; and an x-ray intensity detector that is positioned, during a beam intensity monitoring period at a measurement position that is located between the x-ray source and the first side of the sample, so as to detect at least a part of the x-ray beam before the x-ray beam reaches the sample.
    Type: Grant
    Filed: July 4, 2019
    Date of Patent: November 23, 2021
    Assignee: BRUKER TECHNOLOGIES LTD.
    Inventors: Alex Dikopoltsev, Matthew Wormington, Yuri Vinshtein, Alexander Krokhmal
  • Patent number: 11175288
    Abstract: The invention concerns a method for detecting indicators for determining diseases (disease indicators), in which aggregates of misfolded proteins play a role, and a method for selective quantitation and/or characterization of these disease indicators.
    Type: Grant
    Filed: June 25, 2014
    Date of Patent: November 16, 2021
    Assignee: FORSCHUNGSZENTRUM JUELICH GMBH
    Inventors: Dieter Willbold, Susanne Aileen Funke, Eva Birkmann, Kateryna Kravchenko, Oliver Bannach, Carsten Korth, Verian Bader, Steffen Huebinger
  • Patent number: 11150185
    Abstract: The present technology is directed to the nanoparticles for use as molecular environmental sensors. The nanoparticles comprise a photoluminescence core and a plurality of ligands bound to the core and forming a quencher permeable ligand shell. The ligands comprise a reactive or charged moiety capable of being modulated between a first stand and a second state, and the proportion of ligands in each state determine the permeability of the ligand shell to a photoluminescence quencher.
    Type: Grant
    Filed: June 25, 2018
    Date of Patent: October 19, 2021
    Assignee: Northwestern University
    Inventors: Chen He, David J. Weinberg, Emily A. Weiss, Jeremiah Yoonsung Kim, Chen Wang, Andrew Lee
  • Patent number: 11131630
    Abstract: For setting a laser-scanning fluorescence microscope to a correct alignment in which an intensity maximum of excitation light and an intensity minimum of fluorescence inhibition light coincide in a focal area of an objective lens, a structure in a sample marked with a fluorescent dye is scanned with the intensity maximum of the excitation light to generate first and second pictures of the sample, the first picture corresponding to a higher and the second picture corresponding to a lower intensity of the fluorescence inhibition light. A spatial offset of a first image of the structure in the first picture with regard to a second image of the structure in the second picture is calculated; and the intensity maximum of the excitation light is shifted with regard to the intensity minimum of the fluorescence inhibition light in the direction of the offset calculated to set the microscope to the correct alignment.
    Type: Grant
    Filed: February 26, 2019
    Date of Patent: September 28, 2021
    Assignee: ABBERIOR INSTRUMENTS GMBH
    Inventors: Joern Heine, Matthias Reuss, Benjamin Harke, Lars Kastrup
  • Patent number: 11131622
    Abstract: An information processing device includes a statistical processing unit that performs statistical processing for a group of spectra obtained by applying light to a group of microparticles that exhibit one response property with respect to light, and on a basis of a result of the statistical processing, exclude a spectrum indicating an outlier from the group of spectra, and a reference spectrum calculation unit that calculates a reference spectrum using the group of spectra from which the spectrum indicating the outlier has been excluded.
    Type: Grant
    Filed: February 10, 2017
    Date of Patent: September 28, 2021
    Assignee: SONY CORPORATION
    Inventors: Tomoyuki Umetsu, Yasunobu Kato, Nobuhiro Hayashi, Naoki Ide
  • Patent number: 11131631
    Abstract: A microscopy method and system includes obtaining a plurality of raw fluorescence images, respective ones of the plurality of raw fluorescence images obtained by exciting a sample using a light source at a respective irradiance value in a weak saturation region of the sample, and capturing the respective raw fluorescence image; applying a plurality of weights to the plurality of raw fluorescence images in a one-to-one correspondence so as to generate a plurality of weighted fluorescence images, wherein a respective weight is based on the respective irradiance value at which the corresponding raw fluorescence image was obtained; and linearly combining the plurality of weighted fluorescence images, thereby generating the output image having a resolution greater than a diffraction limit. Respective raw fluorescence images correspond to irradiance values different from one another.
    Type: Grant
    Filed: December 28, 2018
    Date of Patent: September 28, 2021
    Assignee: UNIVERSITY OF NOTRE DAME DU LAC
    Inventors: Scott S. Howard, Yide Zhang, Cody J. Smith
  • Patent number: 11118221
    Abstract: Disclosed herein in are methods and systems for determining genetic variants (e.g., copy number variation) in a polynucleotide sample. A method for determining copy number variations includes tagging double-stranded polynucleotides with duplex tags, sequencing polynucleotides from the sample and estimating total number of polynucleotides mapping to selected genetic loci. The estimate of total number of polynucleotides can involve estimating the number of double-stranded polynucleotides in the original sample for which no sequence reads are generated. This number can be generated using the number of polynucleotides for which reads for both complementary strands are detected and reads for which only one of the two complementary strands is detected.
    Type: Grant
    Filed: January 7, 2020
    Date of Patent: September 14, 2021
    Assignee: GUARDANT HEALTH, INC.
    Inventors: AmirAli Talasaz, Stefanie Ann Ward Mortimer, Helmy Eltoukhy
  • Patent number: 11112593
    Abstract: In some instances, an apparatus can include a light sensitive imaging sensor having a surface to receive a fluid sample, a body to be moved relative to the light sensitive imaging sensor and having a surface to touch a portion of the fluid sample, and a carrier to move the body toward the surface of the light sensitive imaging sensor to cause the surface of the body to touch the portion of the fluid sample, so that as the surface of the body touches the portion of the fluid, the surface of the body (i) is parallel to the surface of the light sensitive imaging sensor, and (ii) settles on top of the fluid sample independently of motion of the carrier.
    Type: Grant
    Filed: February 28, 2020
    Date of Patent: September 7, 2021
    Inventor: Alan Marc Fine
  • Patent number: 11103861
    Abstract: Disclosed are N-heterocyclic carbene (NHC) and 4-pyridinol-derived pincer ligands and metal complexes containing these ligands. These compounds can be used to photocatalyticaly reduce CO2 to CO.
    Type: Grant
    Filed: September 14, 2018
    Date of Patent: August 31, 2021
    Assignees: The Board of Trustees of The University of Alabama, University of Mississippi
    Inventors: Elizabeth T. Papish, Jared Heath Delcamp
  • Patent number: 11084839
    Abstract: Provided herein are a symmetrical pincer metal and bimetallic complexes. The symmetrical pincer metal complex includes a structure according to Formula I: wherein M is a metal; each N and N? is independently nitrogen or carbon; Z is selected from the group consisting of CH, C, and N; n is 0-3; each L is independently a neutral or charged ligand; and each R is independently an alkyl, Nx, CH2TMS. The symmetrical bimetallic complex includes a structure according to Formula II: wherein M is a metal; each N and N? is independently nitrogen or carbon; Z is selected from the group consisting of CH, C, and N; n is 0-3; each L is independently a neutral or charged ligand; and wherein each R is independently an alkyl, Nx, CH2TMS. Also provided herein is a method of catalyzing a reaction including administering one or more of the compounds disclosed herein.
    Type: Grant
    Filed: July 16, 2018
    Date of Patent: August 10, 2021
    Assignees: Mississippi State University, University of Mississippi
    Inventors: Thedford Keith Hollis, Charles Edwin Webster, Jared H. Delcamp
  • Patent number: 11086119
    Abstract: A fluorescence-lifetime imaging microscopy method with time-correlated single-photon counting includes using excitation light pulses separated in each case by a measurement interval to excite a sample to emit fluorescence photons. A detector signal that represents the captured fluorescence photons is generated. Detection times are determined based on the detector signal. Imaging is performed based on the detection times. The detection times of all captured fluorescence photons are compiled in a first data memory, common to a plurality of image pixels. The detection times of only those fluorescence photons which were captured in a predetermined number within the respective measurement intervals are compiled in a second data memory, common to the same plurality of image pixels. The detection times compiled in the data memories are combined within a calculation step. The results of the calculation step are stored in a third data memory.
    Type: Grant
    Filed: December 18, 2018
    Date of Patent: August 10, 2021
    Assignee: LEICA MICROSYSTEMS CMS GMBH
    Inventors: Frank Hecht, Bernd Widzgowski
  • Patent number: 11079373
    Abstract: Aspects of the present disclosure include methods that include co-culturing a cell and a microparticle that includes a capture ligand, in a culture medium under conditions in which a biomarker produced by the cell is bound by the capture ligand. Such methods may further include detecting (e.g., by flow or mass cytometry) complexes that include the microparticle, the capture ligand, the biomarker, and a detection reagent. The methods may further include determining the proportion or number of cells among a heterogeneous cell population that produced the biomarker and/or the level of biomarker secreted by such cells. Compositions, systems and kits are also provided.
    Type: Grant
    Filed: November 26, 2019
    Date of Patent: August 3, 2021
    Assignee: The Board of Trustees of the Leland Stanford Junior University
    Inventors: Dolly B. Tyan, Ge Chen
  • Patent number: 11067552
    Abstract: A multi-core sensor system is provided. The multi-core sensor system can intelligently determine whether the reason for an abrupt dramatic change in sensor data is a sub-sensor fault or sudden pollution, so as to increase reliability of detected data of the sub-sensor. The multi-core sensor system can automatically determine whether the repair is needed when a device fault occurs, so as to ensure the continuity of sub-sensor detected data, which has significant value for continuous monitoring required for a haze treatment operation. In addition, human and material resources for device maintenance may be saved, thereby reducing waste.
    Type: Grant
    Filed: July 31, 2020
    Date of Patent: July 20, 2021
    Assignee: NOVA FITNESS CO., LTD.
    Inventors: Shitian Kou, Michael Jun Xu, Shuaishuai Jia, Jiangshan Song, Shuchun Si
  • Patent number: 11059977
    Abstract: Provided is a near-infrared-absorbing dye increasing a visible light transmittance and having a near-infrared blocking characteristic. The near-infrared-absorbing dye has an absorption characteristic measured by dissolving the dye in dichloromethane satisfying the following requirements. ?In an absorption spectrum at a wavelength of 400 to 800 nm, there is a maximum absorption wavelength ?max in a wavelength region of 670 nm or more. ?The following relational expression is established between a maximum absorption constant ?A with respect to light with a wavelength of 430 to 550 nm and a maximum absorption constant ?B with respect to light with a wavelength of 670 nm or more, where ?B/?A?65. ?In a spectral transmittance curve, an average transmittance of light with a wavelength of 430 to 460 nm is 94.0% or more when a transmittance at the maximum absorption wavelength ?max is set to 1%.
    Type: Grant
    Filed: August 2, 2018
    Date of Patent: July 13, 2021
    Assignee: AGC Inc.
    Inventors: Noriaki Miyake, Satoshi Okada, Jun Irisawa, Teppei Konishi, Keigo Matsuura
  • Patent number: 11035844
    Abstract: An image processing device (2A) comprises: an input means for inputting a brightfield image representing cell morphology in a tissue section, and a fluorescence image representing, by fluorescent bright spots, the expression of a specific protein in the same range of the tissue section; a first generation means for generating a cell image obtained by extracting a specific site of a cell from the brightfield image; a second generation means for generating an image obtained by extracting bright spot regions from the fluorescence image, creating a brightness profile for each bright spot region, and generating a fluorescent particle image obtained by extracting the fluorescent particles in the bright spot regions on the basis of the fluorescence profile for one fluorescent particle, which serves as a fluorescence bright spot source; and a calculation means for superimposing the cell image and the fluorescent particle image on one another.
    Type: Grant
    Filed: July 18, 2019
    Date of Patent: June 15, 2021
    Assignee: KONICA MINOLTA, INC.
    Inventors: Yasuhiro Watanabe, Shuji Ichitani, Kohsuke Gonda, Noriaki Ohuchi, Mika Watanabe
  • Patent number: 11009463
    Abstract: Fluorescence imaging based on stimulated emission. In one example a system includes an excitation source (110) that excites fluorescent markers (122) in a sample (120) with an excitation beam (112), a stimulation source (130) that stimulates the excited fluorescent markers (122) to produce stimulated emission (124), and an objective lens (150) that receives the stimulation beam (132) and the stimulated emission (124). The system further includes a phase shifting and attenuation apparatus (200) configured to receive the stimulation beam (132) and the stimulated emission (124) from the objective lens (150), to attenuate the stimulation beam (132), and to induce a relative phase shift between a reference beam (optionally being at least a portion of the stimulation beam) and the stimulated emission, thereby causing constructive interference between the reference beam and the stimulated emission.
    Type: Grant
    Filed: April 11, 2018
    Date of Patent: May 18, 2021
    Assignee: CALICO LIFE SCIENCES LLC
    Inventors: Andrew Gregory York, Sanjay Ramesh Varma
  • Patent number: 11009400
    Abstract: Aspects of the present disclosure include methods for spectrally resolving light from fluorophores having overlapping fluorescence spectra in a sample. Methods according to certain embodiments include detecting light with a light detection system from a sample having a plurality of fluorophores having overlapping fluorescence spectra and spectrally resolving light from each fluorophore in the sample. In some embodiments, methods include estimating the abundance of one or more of the fluorophores in the sample, such as on a particle. In certain instances, methods include identifying the particle in the sample based on the abundance of each fluorophore and sorting the particle. Methods according to some embodiments includes spectrally resolving the light from each fluorophore by calculating a spectral unmixing matrix for the fluorescence spectra of each fluorophore. Systems and integrated circuit devices (e.g., a field programmable gate array) for practicing the subject methods are also provided.
    Type: Grant
    Filed: December 23, 2019
    Date of Patent: May 18, 2021
    Assignee: Becton, Dickinson and Company
    Inventors: Peter Mage, Keegan Owsley
  • Patent number: 10995914
    Abstract: Embodiments of the present disclosure describe a white light illumination system using InGaN-based orange nanowires (NWs) LED, in conjunction with a blue LD for high speed optical wireless communications. By changing the relative intensities of an ultrabroad linewidth orange LED and narrow-linewidth blue LD components, a hybrid LED/LD device achieves correlated color temperature (CCT) ranging from 3000 K to above 6000K with color rendering index (CRI) values reaching 83.1. Orange-emitting NWs LED are utilized as an active-phosphor, while a blue LD was used for both color mixing and optical wireless communications.
    Type: Grant
    Filed: November 18, 2019
    Date of Patent: May 4, 2021
    Assignee: KING ABDULLAH UNIVERSITY OF SCIENCE AND TECHNOLOGY
    Inventors: Boon S. Ooi, Bilal Janjua, Chao Shen, Chao Zhao, Tien Khee Ng
  • Patent number: 10993646
    Abstract: Systems, devices, kits, and methods are provided herein in the form of example embodiments that relate to calibration of medical devices. The medical devices can be sensors adapted to sense a biochemical attribute. The embodiments can be used to determine calibration information specific to an individual medical device. The embodiments can determine the calibration information by reference to one or more parameters obtained during manufacturing of the medical device. The embodiments can also determine the calibration information by reference to in vitro testing of the medical devices. The embodiments also apply to systems incorporating those medical devices in their use in the field. Also described are embodiments of modifications to surfaces of sensor substrates, such as through applied radiation and/or the creation of a well, to aid in the placement and/or sizing of a sensor element on the substrate.
    Type: Grant
    Filed: August 17, 2018
    Date of Patent: May 4, 2021
    Assignee: Abbott Diabetes Care Inc.
    Inventors: Steven Scott, Christopher J. Bennell, Claire Bhogal, Katie A. Cranfield, Marc B. Taub, Bree M. Winter, Owen D. Reynolds, Jean-Pierre Babka, Dharmendra S. Patel, Christopher A. Thomas, Udo Hoss, Mark S. Yahnke, Tahir S. Kahn
  • Patent number: 10983271
    Abstract: Some embodiments are directed to optical conductors comprising one or more fibrillar organic supramolecular species including an association of triarylamines, methods of preparation and applications thereof as optical and plasmonic waveguides.
    Type: Grant
    Filed: January 27, 2017
    Date of Patent: April 20, 2021
    Assignees: CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE, UNIVERSITÉ DE STRASBOURG
    Inventors: Nicolas Giuseppone, Joseph John Armao, IV, Gero Decher
  • Patent number: 10955349
    Abstract: A fluorescence observation device for observing fluorescence from an observed subject including a negative switching fluorescent substance that undergoes a transition from a deactivated state to an activated state triggered by activation light, and is excited by pump light when in the activated state includes: a first intensity-modulating unit that intensity-modulates the activation light at a frequency f1; a second intensity-modulating unit that intensity-modulates probe light at a frequency f3 different from the frequency f1, the probe light inducing stimulated emission of the observed subject; a light-receiving unit that receives fluorescence from the observed subject irradiated with the pump light, and the intensity-modulated probe light and activation light; and a sensing unit that senses a component with a frequency of f1±f3 in a reception-light signal from the light-receiving unit.
    Type: Grant
    Filed: April 30, 2019
    Date of Patent: March 23, 2021
    Assignee: NIKON CORPORATION
    Inventor: Fumihiro Dake