Methods Patents (Class 250/459.1)
  • Publication number: 20150021496
    Abstract: A method to assist gel analysis and processing and apparatus for the same is disclosed. The method employs a tracing/marking/measuring/analyzing and referencing template of transparent sheet material comprising of a pattern made of vertical and horizontal lines that form quadrilateral shapes upon intersection. The vertical and horizontal lines are clearly visible in white light and ultraviolet light. The template sheet(s) can be inserted in a square shaped template holding assembly (70) which is used in between a UV trans-illuminator (56) and a macromolecule resolving gel matrix (54) such as agarose or polyacrylamide gels. The template pattern can be made on one sheet of transparent material or separate sheets may contain vertical and horizontal lines and the finalized pattern can be achieved by inserting all the separate sheets in the template holding assembly.
    Type: Application
    Filed: July 17, 2013
    Publication date: January 22, 2015
    Inventor: Muhammad Imran Shabbir
  • Publication number: 20150015252
    Abstract: The invention provides methods and kits for detecting the presence of, the amount of or the concentration of a substantially non-emitting metal in a sample by a) providing a sensitizer to a chelator of the substantially non-emitting metal thereby creating a sensitized-metal complex; b) adding an unsensitized chelate, and c) detecting a signal from the unsensitized chelate. The substantially non-emitting metal may be gadolinium and may exist in complex with a chelator. The unsensitized chelate may be terbium (Tb) or europium (Eu), and the detecting a signal may be performed by fluorescence.
    Type: Application
    Filed: March 6, 2013
    Publication date: January 15, 2015
    Inventors: Stewart Russell, Edward A. Fisher, Youssef Zaim Wadghiri, Ryan Casey
  • Patent number: 8933418
    Abstract: A sample observation apparatus includes excitation light irradiation unit to irradiate sample with excitation light; excitation light modulator to modulate spatial intensity distribution of the excitation light on the sample; excitation light modulation control unit to control the excitation light modulator according to modulation control signal; photo detection unit to detect light emission from the sample and to generate a detection signal; image generation unit to generate image data of the sample according to the modulation control signal and the detection signal; modulation control signal generation unit to generate the modulation control signal such that Nyquist frequency of the image data will be larger than cut-off frequency in the spatial intensity distribution of the excitation light on the sample; and image processing unit to emphasize high-frequency component that exceeds the cut-off frequency included in the image data.
    Type: Grant
    Filed: July 3, 2012
    Date of Patent: January 13, 2015
    Assignee: Olympus Corporation
    Inventor: Shinichi Hayashi
  • Publication number: 20150008162
    Abstract: A method for detecting mentholated tobacco, comprising irradiating tobacco containing menthol and a fluorescent taggant with radiation and observing the tobacco for fluorescence from the taggant. A system and method for detecting and separating mentholated tobacco from non-mentholated tobacco within a product stream is also provided.
    Type: Application
    Filed: September 24, 2014
    Publication date: January 8, 2015
    Inventor: Edmond J. Cadieux, Jr.
  • Publication number: 20150008340
    Abstract: A fluorescence observation method of the present invention for detecting plural types of fluorescence emitted from two or more kinds of fluorescent molecules includes: subjecting each of the two or more kinds of fluorescent molecules to multi-photon excitation by exciting light having an exciting wavelength equal to or shorter than 700 nm in a visible region, to generate fluorescence upon making use of an absorption wavelength band in a deep ultraviolet region of each of the two or more kinds of fluorescent molecules; and simultaneously detecting plural types of fluorescence generated on a shorter-wavelength side or on both of the shorter-wavelength side and a longer-wavelength side of the exciting wavelength of the exciting light.
    Type: Application
    Filed: September 24, 2014
    Publication date: January 8, 2015
    Applicant: OLYMPUS CORPORATION
    Inventors: Katsumasa FUJITA, Takeharu NAGAI, Kenta SAITO, Masahito YAMANAKA, Shinichi TAKIMOTO
  • Publication number: 20150011406
    Abstract: The present invention includes a method and system for enhancing the signal-to-noise ratio in emission detection comprising: selecting a probe capable of at least one of fluorescence, phosphorescence, or delayed fluorescence in or about a sample that comprises interfering background signal; and exposing the probe to one or more controllable bursts, each burst comprising two or more pulses, wherein the one or more controllable bursts of high repetition energy pulses enhance the signal from the probe above that of the background signal.
    Type: Application
    Filed: July 3, 2014
    Publication date: January 8, 2015
    Inventors: Ryan M. Rich, Ignacy Gryczynski, Julian Borejdo, Zygmunt Gryczynski
  • Patent number: 8927294
    Abstract: A method of determining a normalized quantity of an analyte adhering to beads in a detection area of a bead-based assaying system, the method comprising: a) causing a complex of the analyte to fluorescently or chemically emit a first light, or to release a dye; b) measuring an integrated intensity of the first light emitted from the beads in the detection area, or a concentration of the dye released from the beads in the detection area, or both; c) causing light to interact with the beads in the detection area, the interaction not depending on whether or how much analyte is adhering to the beads; d) measuring a second light resulting from the interaction with the beads which does not depend on the analyte; and e) determining the normalized quantity of analyte from the integrated intensity of the first light or concentration of the dye or both, and from the measured second light.
    Type: Grant
    Filed: November 29, 2010
    Date of Patent: January 6, 2015
    Assignee: Bio-Rad Laboratories Inc.
    Inventors: Boaz Ran, Chanan Sluszny, Shay Nimri
  • Patent number: 8927944
    Abstract: A method of performing a hot test of a wafer-level, packaged high-brightness phosphor converted light-emitting diode (pc-HBLED) includes selectively heating portions of the phosphor layer using a laser to provide a predetermined temperature gradient in the phosphor layer. The selective heating can directly heat the silicone in a silicone-based phosphor layer, or directly heat the active ion(s) of the phosphor in a Lumiramic™-based phosphor or even the active ion(s) of a silicone-based phosphor layer. A current is applied to the InGaN film to establish a predetermined temperature at the InGaN film junction, the film junction being adjacent to the phosphor layer. Photometric measurements are performed on the HBLED after the selective heating and during the applied electroluminescent current. This method quickly establishes the temperatures and temperature gradients in the HBLED consistent with those of an operating, product-level HBLED, thereby ensuring accurate binning of the HBLED.
    Type: Grant
    Filed: November 9, 2012
    Date of Patent: January 6, 2015
    Assignee: KLA-Tencor Corporation
    Inventor: Richard W. Solarz
  • Publication number: 20150001420
    Abstract: A method for classifying a tissue sample of a biopsy specimen into one of a plurality of classes is presented. The method includes receiving a light from at least one location of the tissue sample including a plurality of chromophores, wherein the received light comprises at least one of an attenuated illumination light and a re-emitted light. The method further includes processing a spectrum of the received light to determine a feature for each of the chromophores in the at least one location of the tissue sample. In addition, the method includes estimating a Z-score for each of the chromophores based on the determined feature. Also, the method includes classifying the tissue sample into one of the plurality of classes based on the estimated Z-score for each of the chromophores.
    Type: Application
    Filed: June 26, 2013
    Publication date: January 1, 2015
    Inventors: Rajesh Veera Venkata Lakshmi Langoju, Abhijit Vishwas Patil, Sridhar Dasaratha, Vignesh Thirukazhukundram Subrahmaniam, Dmitry Vladimirovich Dylov, Siavash Yazdanfar, Stephen B. Solomon
  • Publication number: 20150001421
    Abstract: Defect detection and photoluminescence measurement of a sample directing a beam of oblique-illumination wavelength light onto a portion of the sample, directing a beam of normal-illumination wavelength light for causing one or more photoluminescing defects of the sample to emit photoluminescent light onto a portion of the sample, collecting defect scattered radiation or photoluminescence radiation from the sample, separating the radiation from the sample into a first portion of radiation in the visible spectrum, a second portion of radiation including the normal-illumination wavelength light, and at least a third portion of radiation including the oblique-illumination wavelength light, measuring one or more characteristics of the first portion, the second portion or the third portion of radiation; detecting one or more photoluminescence defects or one or more scattering defects based on the measured one or more characteristics of the first portion, the second portion or the third portion of radiation.
    Type: Application
    Filed: March 14, 2014
    Publication date: January 1, 2015
    Applicant: KLA-Tencor Corporation
    Inventor: Romain Sappey
  • Publication number: 20150001422
    Abstract: Nitrogen vacancies in bulk diamonds and nanodiamonds can be used to sense temperature, pressure, electromagnetic fields, and pH. Unfortunately, conventional sensing techniques use gated detection and confocal imaging, limiting the measurement sensitivity and precluding wide-field imaging. Conversely, the present sensing techniques do not require gated detection or confocal imaging and can therefore be used to image temperature, pressure, electromagnetic fields, and pH over wide fields of view. In some cases, wide-field imaging supports spatial localization of the NVs to precisions at or below the diffraction limit. Moreover, the measurement range can extend over extremely wide dynamic range at very high sensitivity.
    Type: Application
    Filed: June 27, 2014
    Publication date: January 1, 2015
    Applicant: Massachusetts Institute of Technology
    Inventors: Dirk Robert Englund, Matthew Edwin Trusheim
  • Patent number: 8922638
    Abstract: A method and an apparatus for imaging a sample structure (34) with a light microscope are described. The sample structure (34) is prepared with markers and an active marker subset is generated in that a part of the markers is brought into a state in which they can be imaged. When a predetermined activation state is present, the sample structure (34) is imaged onto an arrangement (40) of sensor elements which each generate an image signal, these image signals resulting in a single frame of the sample structure (34). The presence of a predetermined activation state is checked in that at least two test single frames are generated at an interval, and the respective change of the image signal between the two test single frames is detected. The presence of the predetermined activation state is determined when the detected changes of the image signals in their entirety exceed a quantity.
    Type: Grant
    Filed: October 18, 2011
    Date of Patent: December 30, 2014
    Assignee: Leica Microsystems CMS GmbH
    Inventor: Jonas Fölling
  • Patent number: 8921810
    Abstract: Described is a portable dosimeter reader that is small in size and light in weight.
    Type: Grant
    Filed: April 7, 2014
    Date of Patent: December 30, 2014
    Assignee: Landauer, Inc.
    Inventors: R. Craig Yoder, Mark S. Akselrod, Kent J. Dillin, Vasiliy V. Fomenko, David F. Johnson, Yoshikazu Tatsumi, Tse-Min Lo
  • Patent number: 8921809
    Abstract: A microscopy device, particularly for use in an imaging fluorescence lifetime microscopy method is provided. The microscopy device comprises an illumination means for generating an illumination beam, an imaging detector for spatially resolved acquisition of an emission radiation emitted by an object to be examined, an illumination beam path between the illumination means and the object to be examined, and a detection beam path between the object to be examined and the detector. The illumination beam path comprises illumination optics which are designed to generate a light sheet of illumination radiation extending transverse to the axis of the illumination beam path, wherein the axis of the detection beam path is oriented substantially perpendicular to a section plane of the light sheet and of the object to be examined. The illumination means comprise a pulsed laser.
    Type: Grant
    Filed: April 4, 2009
    Date of Patent: December 30, 2014
    Assignee: Carl Zeiss Microscopy GmbH
    Inventors: Helmut Lippert, Christopher Power, Christian Dietrich, Benno Radt
  • Patent number: 8922637
    Abstract: A sample-image acquisition apparatus includes: an image taking device; a movement control section; and a sample-image acquisition section.
    Type: Grant
    Filed: February 23, 2011
    Date of Patent: December 30, 2014
    Assignee: Sony Corporation
    Inventor: Koichiro Kishima
  • Publication number: 20140377792
    Abstract: A technique for determining whether or not a fluorescent material exhibits a directionally dependent property, such as anisotropy or chirality, involves illuminating the particle at its excitation wavelength to stimulate fluorescent emission at both a full-frequency (fundamental) wavelength and a half-frequency wavelength. The ratio of the full-frequency signal strength to the half-frequency signal strength provides an indication of the sample's directionally dependent property. This half-frequency spectral analysis can be used to sort anisotropic particles suspended in fluid flowing through a flow cytometer. For instance, the present technique may be used to separate racemic mixtures of chiral enantiomers of cells, pharmaceutical compounds, and other samples.
    Type: Application
    Filed: August 6, 2012
    Publication date: December 25, 2014
    Inventors: Anjan Kr. Dasgupta, Sarita Roy
  • Publication number: 20140374622
    Abstract: The invention provides a high resolution, wide dynamic range, multi-colour detection platform for microfluidic analysers/instruments and methods. The detection platform uses multiple high gain semiconductor optical sensors for the detection of luminescence from cellular or biological samples. The digitized outputs from these sensors are combined and weighted in a signal processing unit, using pre-determined algorithms for each colour, which optimise the resolution in each of these high gain semiconductor optical sensors while extending the dynamic range of the detection platform.
    Type: Application
    Filed: December 21, 2012
    Publication date: December 25, 2014
    Inventors: Donal Cronin, Jerry O'Brien, Lee Barry
  • Publication number: 20140374623
    Abstract: A method for measuring optical properties of particles of a flowable dispersion using a measuring cuvette. As the dispersion flows through the flow chamber of the cuvette, two laser light beams, offset 90 degrees to one another, illuminate the inner chamber of the cuvette and excite fluorescence in a particle. Regardless of the orientation of the particle, the total fluorescing light of the particle provides an accurate measurement of the contents of the cell and balances out form factor errors.
    Type: Application
    Filed: September 9, 2014
    Publication date: December 25, 2014
    Inventor: Wolfgang Goehde
  • Publication number: 20140377890
    Abstract: An apparatus for monitoring deposition rate, an apparatus including the same, for depositing an organic layer, a method of monitoring deposition rate, and a method of manufacturing an organic light emitting display apparatus using the same, are provided. The deposition rate monitoring apparatus for measuring deposition rate of a deposition material discharged from a deposition source, includes: a light source for irradiating light having a wavelength within a photoexcitation bandwidth of the deposition material; a first optical system for irradiating the light emitted from the light source toward the discharged deposition material; a second optical system for collecting the light emitted from the deposition material; and a first light sensor for detecting the amount of the light which is emitted from the deposition material and collected in the second optical system.
    Type: Application
    Filed: November 13, 2013
    Publication date: December 25, 2014
    Applicant: SAMSUNG DISPLAY CO., LTD.
    Inventors: Alexander Voronov, Dmitry Maslov, Gyoo-Wan Han
  • Publication number: 20140366918
    Abstract: Compositions and methods are provided for cleaning management, such as evaluating the cleaning of surfaces. Compositions and methods for controlling or preventing the spreading of biological contamination are also provided. The compositions may include at least one of a fluorescent indicator, an alcohol, and water.
    Type: Application
    Filed: September 25, 2012
    Publication date: December 18, 2014
    Inventors: Katherine E. Pech, Maynard Riley
  • Publication number: 20140367590
    Abstract: The present invention relates to an apparatus for the optical analysis of a sample, also referred to as microscope, which is configured for an optical analysis process having high resolution for the detection of fluorescent molecules. The apparatus and the process applied when using the apparatus are configured for excitation light generated by an excitation light source to be directed onto a sample and the light emitted by the probe is detected. The apparatus and the process are characterized in that the excitation light is synchronized with the detection. The apparatus is characterized in that it has a polarization device which is configured to modulate the polarization of the excitation light with a modulation signal, wherein the modulation signal has or consists of at least one frequency, in particular a pre-determined frequency or several pre-determined superimposed frequencies, or the modulation signal consists of a sequence of signals that has no repetition.
    Type: Application
    Filed: December 5, 2012
    Publication date: December 18, 2014
    Applicant: Technische Universitaet Braunschweig
    Inventor: Peter J. Walla
  • Publication number: 20140367591
    Abstract: A module is provided for a multi-photon imaging system for simultaneously exciting chromophores of a specimen. A first femtosecond laser source emits a first pulsed excitation beam having a repetition rate 1/T and a wavelength ?1 exciting a first chromophores, the absorbed photons originating from the first excitation beam. A second femtosecond laser source emits a second pulsed excitation beam with a wavelength ?2 exciting a second chromophores, the absorbed photons originating from the second beam; the first beam including an “excitation” part exciting the specimen and a “pumping” part acting as a pump beam for exciting the second laser source to synchronize the second laser source with the first laser source. An optical delay line superimposes spatially and temporally the second beam and the excitation part of the first beam to excite at least a third chromophore, the absorbed photons originating from the first and second excitation beams.
    Type: Application
    Filed: February 1, 2013
    Publication date: December 18, 2014
    Inventors: Pierre Mahou, Emmanuel Jean Marc Beaurepaire, Delphine Debarre, Willy Supatto, Jean Louis Martin
  • Publication number: 20140370612
    Abstract: A method and system for using spatially modulated excitation/emission and relative movement between a particle (cell, molecule, aerosol, . . . ) and an excitation/emission pattern are provided. In at least one form, an interference pattern of the excitation light with submicron periodicity perpendicular to the particle flow is used. As the particle moves along the pattern, emission is modulated according to the speed of the particle and the periodicity of the stripe pattern. A single detector, which records the emission over a couple of stripes, can be used. The signal is recorded with a fast detector read-out in order to capture the “blinking” of the particles while they are moving through the excitation pattern. This concept enables light detection with high signal-to-noise ratio and high spatial resolution without the need of expensive and bulky optics.
    Type: Application
    Filed: September 2, 2014
    Publication date: December 18, 2014
    Inventors: Michael Bassler, Peter Kiesel, Oliver Schmidt, Noble M. Johnson
  • Patent number: 8912511
    Abstract: A device for multi-photon fluorescence microscopy for obtaining information from biological tissue is provided. The device comprising a laser unit for generating an excitation radiation, an optical unit which is formed to focus the excitation radiation for generating an optical signal at different locations in or on an object to be examined, and a detector module for detecting the optical signal from the region of the object. The optical unit for generating the optical signal at different locations in or on the object is movable in at least one direction relative to the object.
    Type: Grant
    Filed: June 17, 2010
    Date of Patent: December 16, 2014
    Assignee: W.O.M. World of Medicine AG
    Inventor: Karl-Heinz Guenter Schoenborn
  • Patent number: 8912512
    Abstract: A method for classifying a tissue sample of a biopsy specimen into one of a plurality of classes is presented. The method includes receiving a light from at least one location of the tissue sample including a plurality of chromophores, wherein the received light comprises at least one of an attenuated illumination light and a re-emitted light. The method further includes processing a spectrum of the received light to determine a feature for each of the chromophores in the at least one location of the tissue sample. In addition, the method includes estimating a Z-score for each of the chromophores based on the determined feature. Also, the method includes classifying the tissue sample into one of the plurality of classes based on the estimated Z-score for each of the chromophores.
    Type: Grant
    Filed: June 26, 2013
    Date of Patent: December 16, 2014
    Assignee: General Electric Company
    Inventors: Rajesh Veera Venkata Lakshmi Langoju, Abhijit Vishwas Patil, Sridhar Dasaratha, Vignesh Thirukazhukundram Subrahmaniam, Dmitry Vladimirovich Dylov, Siavash Yazdanfar, Stephen B. Solomon
  • Publication number: 20140363091
    Abstract: Exemplary embodiments of the present invention disclose a method for detecting a tampering event of a component. In a step, an exemplary embodiment encapsulates a fluorescent dye in one or more microcapsules. In another step, an exemplary embodiment embeds the one or more microcapsules in a translucent polymeric resin. In another step, an exemplary embodiment secures at least part of a component in the translucent polymeric resin. In another step, an exemplary embodiment detects a fluorescence of the fluorescent dye from a microcapsule in the translucent polymeric resin that is ruptured during a tampering with a light source that causes the fluorescent dye to fluoresce.
    Type: Application
    Filed: June 6, 2013
    Publication date: December 11, 2014
    Inventors: Dylan J. Boday, Jason T. Wertz
  • Patent number: 8906698
    Abstract: A method of measuring the fluorescence of a fluorescent marker compound dissolved or dispersed in a bulk material includes: (a) measuring a characteristic of the fluorescence of a mixture of said bulk material and said fluorescent marker compound; (b) quenching the fluorescence of the fluorescent marker compound to produce a quenched mixture; (c) measuring the characteristic of the fluorescence of the quenched mixture; (d) comparing the fluorescent characteristic of the mixture with the fluorescent characteristic of the quenched mixture; and (e) correcting the measured fluorescent emission characteristic for the effects of the absorbance of the bulk material. The measurement may be further corrected to account for the absorbance of the material which is also known to have an effect on the measured fluorescence. A method of tagging and identifying a bulk material with a fluorescent marker compound, and an apparatus for carrying out the methods are also described.
    Type: Grant
    Filed: February 2, 2010
    Date of Patent: December 9, 2014
    Assignee: Johnson Matthey PLC
    Inventors: Vincent Brian Croud, Duncan William John McCallien, Ian Stuart Edworthy
  • Publication number: 20140353523
    Abstract: A method for inspecting a polysilicon layer includes: radiating excitation light to the polysilicon layer; and detecting a photoluminescence signal generated by the excitation light, wherein average power of the excitation light has a range of 1 W/cm2 to 10 W/cm2, and peak power of the excitation light has a range of 100 W/cm2 to 1000 W/cm2.
    Type: Application
    Filed: December 2, 2013
    Publication date: December 4, 2014
    Applicant: Samsung Display Co., Ltd.
    Inventors: Alexander VORONOV, Suk-Ho LEE, Jae-Seung YOO, Kyung-Hoe HEO, Gyoo-Wan HAN
  • Publication number: 20140353524
    Abstract: Embodiments of the present invention are directed to imaging technologies, and, in particular, to an imaging system that detects relatively weak signals, over time, and that uses the detected signals to determine the positions of signal emitters. Particular embodiments of the present invention are directed to methods and systems for imaging fluorophore-labeled samples in order to produce images of the sample at resolutions significantly greater than the diffraction-limited resolution associated with optical microscopy. Embodiments of the present invention employ overlapping-emitter-image disambiguation to allow data to be collected from densely arranged emitters, which significantly decreases the data-collection time for producing intermediate images as well as the number of intermediate images needed to computationally construct high-resolution final images. Additional embodiments of the present invention employ hierarchical image-processing techniques to further resolve and interpret disambiguated images.
    Type: Application
    Filed: August 18, 2014
    Publication date: December 4, 2014
    Applicant: GE HEALTHCARE BIO-SCIENCES CORP.
    Inventors: GAUDENZ DANUSER, PAUL C. GOODWIN
  • Patent number: 8901514
    Abstract: System, including methods and apparatus, for sample analysis using at least one array of spotlights to illuminate a microplate with excitation light for photoluminescence emission.
    Type: Grant
    Filed: June 28, 2012
    Date of Patent: December 2, 2014
    Assignee: Molecular Devices, LLC
    Inventor: Chun-Shen Lee
  • Patent number: 8901515
    Abstract: A method for nondestructive testing of workpiece surfaces by a fluorescent penetration test is disclosed. An embodiment of the method includes a) cleaning the area of the workpiece surface that is to be inspected; b) applying a fluorescent liquid penetrant to the area of the workpiece surface that is to be inspected, where the penetrant penetrates into possible recesses in the workpiece surface; c) removing the excess penetrant from the workpiece surface; d) applying a developer to the area of the workpiece surface that is to be inspected; e) bleaching the fluorescent penetrant by a beam of light in the layer formed by applying the developer to the workpiece surface; and f) visual evaluation of the fluorescent penetrant remaining in the recesses present in the workpiece surface.
    Type: Grant
    Filed: January 18, 2013
    Date of Patent: December 2, 2014
    Assignee: MTU Aero Engines GmbH
    Inventors: Joachim Bamberg, Wilhelm Satzger
  • Patent number: 8901513
    Abstract: A system or method for analyzing a sample include an input light source, a double subtractive monochromator positioned to receive light from the input light source and to sequentially illuminate the sample with each of a plurality of wavelengths, a multi-channel fluorescence detector positioned to receive and substantially simultaneously detect multiple wavelengths of light emitted by the sample for each of the plurality of excitation wavelengths, an absorption detector positioned to receive and detect light passing through the sample, and a computer in communication with the monochromator, the fluorescence detector, and the absorption detector, the computer controlling the monochromator to sequentially illuminate the sample with each of the plurality of wavelengths while measuring absorption and fluorescence of the sample based on signals received from the fluorescence and absorption detectors.
    Type: Grant
    Filed: March 8, 2011
    Date of Patent: December 2, 2014
    Assignee: Horiba Instruments, Incorporated
    Inventors: Adam M. Gilmore, Xiaomei Tong
  • Publication number: 20140346371
    Abstract: Fluorescing gel formulations are disclosed for monitoring cleaning of a surface. The fluorescing gel formulations are stable, fluoresce under UV light, and do not leave a mark after drying and removal. The compositions include an oppositely charged complexing agent which is used in combination with an anionic or cationic optical brightener. In some embodiments, the compositions include a cationic optical brightener with no complexing agent.
    Type: Application
    Filed: August 6, 2014
    Publication date: November 27, 2014
    Inventors: Nicole Burns, Xin Sun, Thomas R. Mohs, Joseph R. Wegner, Mark Levitt, Steven E. Lentsch
  • Patent number: 8895941
    Abstract: Depth-sensitive fluorescent spectroscopy can be executed by directing UV radiation through a face of a laminated glass sheet to induce distinct fluorescence in respective target layers of the laminated glass sheet. The respective target layers define glass compositions and relative indices of refraction that permit formation of an externally-viewable fluorescent intensity profile across the target layers of the laminated glass sheet. In an alternative embodiment, non-UV laser radiation is directed from a non-UV laser radiation source through a face of the laminated glass sheet to define a series of multi-photon focal points in the laminated glass sheet and induce fluorescence in respective ones of the plurality of target layers of the laminated glass sheet at a UV excitation frequency that exceeds the frequency of the radiation source.
    Type: Grant
    Filed: February 5, 2013
    Date of Patent: November 25, 2014
    Assignee: Corning Incorporated
    Inventor: Stephan Lvovich Logunov
  • Publication number: 20140339439
    Abstract: A method for high-resolution 3D localization microscopy, in which sample is used which has a boundary surface on the imaging side. The sample is illuminated with excitation light in order to excite fluorescence markers to emit light. The sample is imaged to a still image along an imaging direction, by means of imaging optics. The still image contains images of the fluorescing fluorescence markers. The imaging optics has a focal plane and an optical resolution. The excitation and imaging steps are repeated multiple times so that multiple still images are obtained. The excitation steps create images of at least a subset of the fluorescing fluorescence markers isolated in each of the still images. A location is determined in each of the still images and this location has a precision which is greater than the optical resolution. A high-resolution composite image is generated from the locations determined in this manner.
    Type: Application
    Filed: May 13, 2014
    Publication date: November 20, 2014
    Applicant: Carl Zeiss Microscopy GmbH
    Inventors: Joerg RITTER, Joerg SIEBENMORGEN, Thomas KALKBRENNER
  • Publication number: 20140339438
    Abstract: The present invention relates to devices and methods for spectrometric analysis of light-emitting samples. The device comprises a particle beam source, which generates a primary particle beam directed to the sample in such a way that the primary particle beam is incident on the sample and photons are released from the sample due to the interaction between primary particle beam and sample material. Moreover, the device comprises a plurality of light-pickup elements, which are suitable for capturing the photons released from the sample, wherein the light-pickup elements capture the photons emitted in the respectively assigned solid-angle range. Furthermore, the device comprises conduction elements, which are embodied to forward captured photons to an evaluation unit, and an analysis system, which comprises a plurality of evaluation units in such a way that photons captured by each light-pickup element are analyzed spectrally.
    Type: Application
    Filed: May 13, 2014
    Publication date: November 20, 2014
    Inventors: Nico Correns, Michael Rode
  • Patent number: 8890089
    Abstract: Described are methods for analyzing an animal carcass, meat obtained therefrom, or product produced by or obtained from an animal for the presence or absence of faecal matter. The methods comprise analyzing an animal carcass, meat obtained therefrom, or product produced by or obtained from the animal for the presence or absence of a detectable marker, the presence of the detectable marker being indicative of the presence of faecal matter and the absence of the detectable marker being indicative of the absence of faecal matter, wherein the animal carcass, meat obtained therefrom, or product produced by or obtained from the animal has been obtained from an animal fed a composition comprising a supplement of the detectable marker and/or a precursor thereof. Also described are compositions for feeding to an animal and for use in the methods.
    Type: Grant
    Filed: March 9, 2010
    Date of Patent: November 18, 2014
    Assignee: Aberystwyth University
    Inventors: Michael Lee, Michael Theodorou, Helen Ougham, Howard Thomas
  • Patent number: 8890077
    Abstract: Various embodiments of the present invention provide a method of detecting inaccessible radiation sources by measuring corresponding ions and excited molecules created by radiation, using LIDAR technology. The LIDAR system of the present invention employs a pulsed laser transmitter, a telescope receiver, and associated control and acquisition systems. Light propagates out from the laser transmitted and is directed into the volume surrounding the radioactive source, or the “ion cloud.” The ion cloud absorbs the transmitted light, which induces the non-fluorescing ions to fluoresce. Light from the ion cloud is then backscattered and the telescope receiver subsequently collects the photons from the backscattered light. The intensity of the fluorescence (determined by the photon count) is measured, which provides an indication of the number density of the ionized atoms. Algorithms can then be used to relate the measured ionization rates to the source activity.
    Type: Grant
    Filed: August 4, 2010
    Date of Patent: November 18, 2014
    Assignee: Georgia Tech Research Corporation
    Inventors: Robert L. Rosson, Bernd Kahn, Brent Wagner, David Roberts
  • Publication number: 20140332697
    Abstract: The method of detecting thalassemia by optical analysis of blood components is a spectral detection method that is based on the fluorescence spectra of a set of biomolecules, including tyrosine, tryptophan, nicotinamide adenine dinucleotide, and flavin adenine dinucleotide, which are all found in blood plasma, and porphyrin, which is found in red blood cells (RBCs). Measured ratios of intensity maxima between tryptophan and nicotinamide adenine dinucleotide, flavin adenine dinucleotide and nicotinamide adenine dinucleotide, tyrosine and tryptophan, and the normal form of porphyrin and the basic form of porphyrin may each be used, alone or in combination, to diagnose a patient as suffering from thalassemia.
    Type: Application
    Filed: May 7, 2013
    Publication date: November 13, 2014
    Applicant: KING SAUD UNIVERSITY
    Inventors: MOHAMAD SALEH AL SALHI, VADIVEL MASILAMANI, FARJAH HASSAN ALGAHTANI
  • Publication number: 20140333925
    Abstract: Methods and apparatus for obtaining an image of light scattering biological tissue. A series of pulses of substantially monochromatic light of a first wavelength is generated and split into two parts, of which one part illuminates a scattering biological tissue at an intensity too low to damage the tissue, while a second part is upconverted to generate a pump beam. Sample light from the biological tissue, which may be scattered (or transmitted) light, or fluorescence, or Raman scattering, etc., is collected and directed from the scattering biological tissue, along with the pump beam, into a non-linear optical element, in a single pass or multiple passes. Parametrically amplified sample light emerging from the non-linear optical element is detected and analyzed or displayed.
    Type: Application
    Filed: January 17, 2014
    Publication date: November 13, 2014
    Applicant: The Board of Trustees of the University of Illinois
    Inventors: Stephen A. Boppart, Youbo Zhao
  • Patent number: 8884211
    Abstract: The invention provides imaging apparatus and methods useful for obtaining a high resolution image of a sample at rapid scan rates. A rectangular detector array having a horizontal dimension that is longer than the vertical dimension can be used along with imaging optics positioned to direct a rectangular image of a portion of a sample to the rectangular detector array. A scanning device can be configured to scan the sample in a scan-axis dimension, wherein the vertical dimension for the rectangular detector array and the shorter of the two rectangular dimensions for the image are in the scan-axis dimension, and wherein the vertical dimension for the rectangular detector array is short enough to achieve confocality in a single axis.
    Type: Grant
    Filed: March 12, 2012
    Date of Patent: November 11, 2014
    Assignee: Illumina, Inc.
    Inventors: Wenyi Feng, Theofilos Kotseroglou, Mark Wang, Alexander Triener, Diping Che, Robert Kain
  • Publication number: 20140326898
    Abstract: System and method for fluorescent light excitation and detection from samples to enhance the numerical aperture and/or reduce the cross-talk of the fluorescent light.
    Type: Application
    Filed: March 31, 2014
    Publication date: November 6, 2014
    Applicant: Applied Biosystems, LLC
    Inventors: Richard REEL, Eric NORDMAN
  • Publication number: 20140326899
    Abstract: A particulate composition comprises a plurality of particles wherein at least one of the particles comprises at least two different crystalline and/or glass phases, each phase comprising a host lattice and a dopant sensitive to electromagnetic radiation. The different phases simultaneously produce different responses on exposure to photons of the same energy, whereby the output from the particulate composition when exposed to said photons is the sum of the responses from the different phases.
    Type: Application
    Filed: June 16, 2014
    Publication date: November 6, 2014
    Applicant: CABOT SECURITY MATERIALS, INC.
    Inventors: Scott T. Haubrich, Jainisha Shah, Steven R. Cordero
  • Publication number: 20140319379
    Abstract: A method and apparatus for three dimensional fluorescent analysis of a target in a liquid sample having fluorescent analyte as a target substance or fluorescent label on the target substance. A rotating carousel carries liquid samples beneath a beam having a beam spot at a horizontal plane of the sample. The carousel advances slowly a linear direction during rotation so that a pattern of overlapping spiral scans provide R-theta two-dimensional samples of the entire plane to identify locations of volumes of interest where fluorescence is measurably higher than background. These locations are probed in the depthwise direction to find locations of fluorescent analyte in three dimensions. Light from the analyte or fluorescent label is detected and processed to characterize the target by color, size or other morphology.
    Type: Application
    Filed: July 9, 2014
    Publication date: October 30, 2014
    Inventor: Bala S. Manian
  • Publication number: 20140319377
    Abstract: A solar concentrator comprising: a light-transmissive sheet including: a plurality of luminescent particles capable of becoming excited by absorbing light within at least a first spectrum of absorption frequencies and, once excited, capable of being stimulated to emit light having a spectrum within at least a first spectrum of emission frequencies; and a first light-guide; and a light source for generating a pulsed probe light having a spectrum, at least a portion of which is within at least the first spectrum of emission frequencies, for stimulating at least one of the excited luminescent particles having absorbed light within the first spectrum of absorption frequencies such that when the probe light traveling in a first direction of travel stimulates the excited luminescent, the excited luminescent particles emit emitted light having a spectrum within the first spectrum of emission frequencies in the first direction of travel of the probe light.
    Type: Application
    Filed: February 6, 2014
    Publication date: October 30, 2014
    Applicant: MORGAN SOLAR INC.
    Inventors: John Paul MORGAN, Pascal DUFOUR
  • Publication number: 20140319378
    Abstract: An optical fluorescence-based sensor comprising at least one sensing element is disclosed. In one aspect, the at least one sensing element comprises a waveguide comprising a waveguide core, a light source optically coupled to an input part of the waveguide core, and a photodetector optically coupled to an output part of the waveguide core, the waveguide core being made of a material comprising a mixture of an optical material and a fluorescent dye.
    Type: Application
    Filed: May 28, 2014
    Publication date: October 30, 2014
    Applicants: IMEC, Universiteit Gent
    Inventors: Geert Van Steenberge, Sandeep Kalathimekkad, Jeroen Missinne
  • Publication number: 20140318401
    Abstract: The present invention relates to a composition having a first response to a first electromagnetic radiation and, after intermediate exposure to a second electromagnetic radiation, a second response to the first electromagnetic radiation, different from the first response. In one aspect, the composition exhibits a regenerated first response to the first electromagnetic radiation after exposure to a third electromagnetic radiation.
    Type: Application
    Filed: July 8, 2014
    Publication date: October 30, 2014
    Inventors: Mark J. Hampden-Smith, Liam Noailles, Richard A. Einhorn
  • Patent number: 8872132
    Abstract: A system of analysis with the naked eye and by fluorescence of a field in an illuminated area comprising a periodically-excited first low-remanence white light illumination source; a second light source for exciting fluorescent elements located in said field, active at least during part of the time periods when the first source is off; and a fluorescence analysis device active during time periods when the first source is off and the second source is on.
    Type: Grant
    Filed: October 30, 2013
    Date of Patent: October 28, 2014
    Assignee: Commissariat a l'Energie Atomique
    Inventors: Jean-Pierre Moy, Philippe Rizo, Anabella Da Silva
  • Patent number: 8872114
    Abstract: A technology of inspecting a photoexcited carrier generation area of a photo device in a non-contact manner is provided. An inspection apparatus inspects a photovoltaic cell panel in which the photo device is formed. The inspection apparatus includes an irradiation part that irradiates the photovoltaic cell panel with pulsed light from a light receiving surface side and a detecting part (detector) that detects electric field intensity of a terahertz wave pulse, which is generated according to the irradiation of the pulsed light.
    Type: Grant
    Filed: June 12, 2012
    Date of Patent: October 28, 2014
    Assignees: Dainippon Screen Mfg. Co., Ltd., Osaka University
    Inventors: Hidetoshi Nakanishi, Masayoshi Tonouchi, Iwao Kawayama
  • Patent number: 8872133
    Abstract: A device for measuring the fluorescence of a medium having a radiation source, an emission-receiving element and an optical imaging element arranged on the sensor side of the optical imaging element, and a scattering-receiving element arranged on the sensor side of the optical imaging element and in which the radiation source, the imaging element and the emission-receiving element are aligned and configured relative to one another so that the medium present on the medium side of the imaging element can be illuminated by radiation from the radiation source, and the emission intensity of the medium radiation emitted by the medium based on fluorescence can be detected with the emission-receiving element. To provide a device for measuring the fluorescence of a medium which has an increased reliability in measuring the fluorescence, temperature compensation is performed relative to the temperature of the medium and/or at least one of the receiving elements.
    Type: Grant
    Filed: May 7, 2010
    Date of Patent: October 28, 2014
    Assignee: KROHNE Optosens GmbH
    Inventors: Marco Schreiber, Thomas Fritsch