Abstract: A spectroscopic microscope includes a laser or other light source which emits light from the entrance aperture of its spectrograph, and also includes a light sensor situated on the microscope sample stage upon which a specimen is to be situated for microscopic/spectrometric analysis. The sample stage is positioned such that the signal from the light sensor is maximized, thereby indicating good alignment between the sample stage and spectrograph. Additionally, the microscope sample stage bears a light source which can emit light to be detected by a light sensor situated at the vantage point of a user/viewer utilizing the microscope, and such a light sensor can simply take the form of a video camera or other image recordation unit associated with the microscope. The sample stage can also be positioned to optimize the signal at the light sensor to signify good alignment between the sample stage and the microscope.

Abstract: Without using an interferometer, small displacement and/or three-dimensional shape of an object is detected in a noncontact way with high accuracy using pseudo-phase information calculated from e.g., a speckle pattern having a spatially random structure. A speckle image of the test object of the before displacement is obtained, and a spatial frequency spectrum is calculated by executing an N-dimensional Fourier transform for this. The complex analytic signal is obtained by setting the amplitude of frequency spectrum in the half plane including zero frequency in this amplitude distribution to zero, and executing the frequency spectrum amplitude in the half plane of the remainder in the inverse Fourier transform. And then, the amplitude value of this complex analytic signal is replaced with the constant value, a part of the obtained analytic signal domain is clipped, the phase information is calculated by the phase-only correlation function, and the cross-correlation peak in N-dimension is obtained.

Abstract: A method comprising obtaining a first set of spectral data for a first sample film measured by a first system, extracting intensities for one or more elemental species associated with the first sample film to provide a first set of extracted intensities using a function, and determining a first quantitative characteristic associated with the first sample film using the first set of extracted intensities. Next, obtain a second set of spectral data measured for a comparable sample film measured by a second photoelectron spectroscopy system. Next, apply the same function and continually adjust the function to extract intensities for the respective elemental species associated with the comparable sample film to provide a second set of corrected-extracted intensities. A second quantitative characteristic for the comparable sample is determined. The function is continually adjusted until the determined second quantitative characteristic closely or substantially matches the first quantitative characteristic.

Abstract: Most scanning systems today employ the conventional prior art method of using mirrors, moving the lens, rotating the slit, and moving the internal optics of the system. This system moves the slit plane across the projected focal plane that is formed by the fore/objective lens. The basic system includes a camera/sensor, a dispersive spectrograph with entrance slit, a computer with control software, an encoded stage and/or motor control, an objective lens, a computer, and an enclosure. The system works as a stationary enclosure with internal moving parts. The spectrograph and camera are moved in precision by a linear stage in the vertical or horizontal plane depending on whether the slit is vertically set or horizontally set. The precision movement of the assembly is controlled via an intelligent motor controller interface and customized software.

Abstract: A digital spectrophotometer and a spectrologial method for determining spectrum wavelength of an unknown illuminant, and the digital spectrophotometer has a base, an operating assembly and a photographic assembly. The operating assembly is attached to the base and has an operating pedestal, a rotating frame and a spectrometer. The rotating frame is connected rotatably to the operating pedestal. The spectrometer is connected solidly to the rotating frame. The photographic assembly is connected to the operating assembly. The spectrologial method is calculated the diffraction angle ? i and the wavelength of the unknown illuminant by putting the parameters into the into the first and second functions.

Abstract: A method and apparatus for imaging using a double-clad fiber is described.

Abstract: This invention comprises the means for the capture of full spectrum images in an electronic camera without the use of color primary filters to limit the spectral color gamut of the captured image. The fundamental principle of the invention is that each pixel of the image sensor acts as an independent spectrophotometer and spectral separator. Electromagnetic energy enters though a slit or collimating optic. Electromagnetic energy gets diffracted into component spectra by diffraction grating spectrophotometer for each pixel of image Electromagnetic energy leaves diffraction grating at different angles based on wavelength of the energy Spectrophotometer separates light for each pixel into its spectral components onto photodetector line array elements. Individual line array elements which are activated determine the original radiance level of the light source containing that specific wavelength region. The sum of these regions determines the spectral signature of the light at that pixel element.

Abstract: A substrate processing system includes a processing module to process a substrate, a factory interface module configured to accommodate at least one cassette for holding the substrate, a spectrographic monitoring system positioned in or adjoining the factory interface module, and a substrate handler to transfer the substrate between the at least one cassette, the spectrographic monitoring system and the processing module.

Abstract: A chemical imaging system is provided which uses a near infrared radiation microscope. The system includes an illumination source which illuminates an area of a sample using light in the near infrared radiation wavelength and light in the visible wavelength. A multitude of spatially resolved spectra of transmitted, reflected, emitted or scattered near infrared wavelength radiation light from the illuminated area of the sample is collected and a collimated beam is produced therefrom. A near infrared imaging spectrometer is provided for selecting a near infrared radiation image of the collimated beam. The filtered images are collected by a detector for further processing. The visible wavelength light from the illuminated area of the sample is simultaneously detected providing for the simultaneous visible and near infrared chemical imaging analysis of the sample. Two efficient means for performing three dimensional near infrared chemical imaging microscopy are provided.

Abstract: The present invention discloses an optical measurement and/or inspection device that, in one application, may be used for inspection of semiconductor devices. It comprises a light source for providing light rays; a half-parabolic-shaped reflector having an inner reflecting surface, where the reflector having a focal point and an axis of summary, and a device-under-test is disposed thereabout the focal point. The light rays coming into the reflector that is in-parallel with the axis of summary would be directed to the focal point and reflect off said device-under-test and generate information indicative of said device-under-test, and then the reflected light rays exit said reflector. A detector receives the exited light rays and the light rays can be analyzed to determine the characteristics of the device-under-test.

Abstract: A chemical imaging system is provided which uses a near infrared radiation microscope. The system includes an illumination source which illuminates an area of a sample using light in the near infrared radiation wavelength and light in the visible wavelength. A multitude of spatially resolved spectra of transmitted, reflected, emitted or scattered near infrared wavelength radiation light from the illuminated area of the sample is collected and a collimated beam is produced therefrom. A near infrared imaging spectrometer is provided for selecting a near infrared radiation image of the collimated beam. The filtered images are collected by a detector for further processing. The visible wavelength light from the illuminated area of the sample is simultaneously detected providing for the simultaneous visible and near infrared chemical imaging analysis of the sample. Two efficient means for performing three dimensional near infrared chemical imaging microscopy are provided.

Abstract: A chemical imaging system is provided which uses a near infrared radiation microscope. The system includes an illumination source which illuminates an area of a sample using light in the near infrared radiation wavelength and light in the visible wavelength. A multitude of spatially resolved spectra of transmitted, reflected, emitted or scattered near infrared wavelength radiation light from the illuminated area of the sample is collected and a collimated beam is produced therefrom. A near infrared imaging spectrometer is provided for selecting a near infrared radiation image of the collimated beam. The filtered images are collected by a detector for further processing. The visible wavelength light from the illuminated area of the sample is simultaneously detected providing for the simultaneous visible and near infrared chemical imaging analysis of the sample. Two efficient means for performing three dimensional near infrared chemical imaging microscopy are provided.

Abstract: Improved ease of mode matching to a passive optical cavity is provided by selecting a cavity design that has a predetermined deviation from a reference cavity design having high transverse mode degeneracy. This predetermined deviation tends to be small, so that the first overlap of high-order transverse modes with the lowest order transverse mode in frequency occurs at relatively high transverse mode numbers. Coupling to high-order transverse modes is thereby reduced, since high-order transverse modes having relatively high transverse mode numbers tend to be more difficult to couple to, and tend to have high loss. During assembly of such a cavity, it can be useful to apply a perturbation to the cavity to further optimize mode matching. For example, the length of an enclosed cavity can be adjusted by altering the number and/or length of spacers in the cavity housing.

Abstract: A system for laser scanning provides spectral flexibility needed for the spectroscopic monitoring of highly multiplexed samples, such as cellular and particle assays in whole blood or other suspensions. In accordance with embodiments of the present invention, the system comprises a scanner to direct an excitation laser through a sample, an objective to collect light emitted by the sample in response to the excitation laser, a spectrograph to disperse the emitted light over a plurality of wavelengths as a spectrum, and a charge coupled device for detecting the spectrum. The system can be used with samples having a variety of reporter tags, including one or more SERS tags, fluorescent organic and protein tags, and quantum dot tags. A laser scanning apparatus and method of using the same is also provided.

Abstract: An analyzing method and system includes projecting light onto a specimen, detecting an optical spectrum from the specimen, dividing the detected optical spectrum by a predetermined time interval, applying a weighting vector and a gain and offset vector to the divided spectra to generate a resolution vector, quantizing an analog signal of the resolution vector to a digital signal, and applying a synthesis vector to the quantized spectra to restore the quantized spectra to a size of the optical spectrum. A spectrum having a large amplitude difference is temporally divided and is analyzed by applying different resolutions to individual time intervals. Spectral portions of the spectrum having relatively small peak amplitudes are selected as a region of interest (ROI) to be modified.

Abstract: A Method for identifying one or a small number of molecules, especially in a dilution of ?1 ?M, using laser excited FCS with measuring times ?500 ms and short diffusion paths of the molecules to be analyzed, wherein the measurement is performed in small volume units of preferably ?10?14 l, by determining material-specific parameters which are determined by luminescence measurements of molecules to be examined. The device which can be preferably used for performing the method according to the invention is a per se known system of microscope optics for laser focusing for fluorescence excitation in a small measuring compartment of a very diluted solution and for imaging the emitted light in the subsequent measurement through confocal imaging wherein at least one system of optics with high numerical aperture of preferably ?1.2 N.A.

Abstract: According to the present invention, the expansion of measurable concentration range for an antigen in a sample solution can be achieved, without a step of dilution or the like. The concentration of an antigen contained in a sample solution is determined from the maximum value and/or minimum value of turbidity level detected in the observation of the transient phenomenon of turbidity, elapsed time when the maximum and/or minimum value is observed, and turbidity level.

Abstract: The present invention relates to interfacing new sensors to incumbent controls. In particular, it relates to optically interfacing a new sensor, such as a spectrometer with plasma generator, to an incumbent electro-optical sensor. Logic and resources to control activation of the incumbent electro-optical sensor may be included. Particular aspects of the present invention are described in the claims, specification and drawings.

Abstract: An ion implanted semiconductor surface is illuminated with a flood illumination of monochromatic radiation, and an image of the surface is taken using light which has been Raman scattered. The illumination and imaging system are calibrated by flood illuminating a uniformly Raman scattering surface.

Abstract: Apparatus for the manipulating and testing of molecules and in particular of DNA comprising a surface on which the molecule is anchored on multiple points at one end and a paramagnetic bead on which said molecule is anchored on multiple points at its other end, magnetic means for applying a force to the bead, said magnetic means being used to control the stretching and rotation of said bead and molecule, optical magnification means and a camera for the visualisation of said bead, computer means to which the images of the camera are transmitted, said computer means comprising means for analyzing the motions of the bead. A method for the manipulating and testing of molecules and in particular of DNA in which a molecule is anchored at one end to a fixed surface and at its other end to a paramagnetic bead wherein said molecule is anchored on multiple points at each of said ends.

Abstract: An apparatus and method of measuring an article is provided. The method includes providing an article having a feature to be measured, the article having a surface; measuring the surface of the article with a measuring instrument to obtain article surface data; and analyzing the article surface feature data such that data on the feature to be measured is developed. Measuring the surface of the article can include scanning the measuring instrument over the article surface. Analyzing the article surface feature data can include associating portions of the article surface data with individual features thereby producing associated feature surface data; and analyzing the associated feature surface data. The measuring instrument can be, for example, a contact measuring instrument or an interference measuring instrument. A computer storage medium having instructions stored therein for causing a computer to perform the method described above is also provided.

Abstract: A method of monitoring sample crystallization from a solution. The method includes the collection of multiple Raman spectra from a sample dissolved in a solvent as a function of time and under conditions promoting crystallization. Within each of the multiple Raman spectra, a first signal is identified corresponding to the sample associated with the solvent. A second signal corresponding to the sample in a microcrystallite state is also identified. Thereafter, the intensity of the multiple Raman spectra are measured for an increase relating to formation of the sample in a microcrystallite state. A method of monitoring sample crystallization from a solution as a function of turbidity is also disclosed. The method includes the collection of multiple Raman spectra from a sample dissolved in a solvent as a function of time under conditions promoting crystallization.

Abstract: In a method, a device and a computer program for determining the blood flow in a tissue or organ region, the fluorescence intensity of an exogenous chromophore is measured as a function of time in the tissue region to be examined and from this the permeation rate of the chromophore is calculated, from which the blood flow can be derived.

Abstract: A produce recognition system and method which use an internal reference to calibrate a produce data collector. The produce data collector collects first data from an external reference, collects second and third data from an internal reference, and collects fourth data from a produce item. A computer determines a first calibration value from the first and second data and a second calibration value from the third data and applies the first and second calibration values to the fourth data to produce fifth data. The computer further obtains sixth data from reference produce data and compares the fifth and sixth data to identify the produce item.

Abstract: A system for the detection of bacteria based on bacteria-antibody complexes. Bacteria attached to antibody are detected with resonance Raman spectroscopy. The bacteria are detected directly in a great numerical excess, e.g. 100 to 10,000 of antibody molecules. A sample to be tested is placed in a medium, the medium containing antibodies attached to a surface for binding to a specific bacteria to form an antigen to antibody complex. The medium is contacted with a beam of light energy. The bacteria, as a lower resonance enhanced Raman backscattered energy, is analyzed for the presence or absence of the bacteria.

Abstract: A hyperspectral scene generator generates a projected linear scene where the spectral characteristics at each location that forms the scene are dynamically and arbitrarily controllable. The generator can be controlled to generate a projected linear scene including a targeted object and arbitrary spectral content that duplicates the spectral content of real targets and backgrounds to facilitate testing of target identification software of a hyperspectral imaging system in view of expected actual field operation of the sensor of the imaging system.

Abstract: According to one embodiment of the present invention, identifying a molecule of a sample includes illuminating the sample with a preparation light beam, where the preparation light beam can initiate a substantially maximized coherence of a target molecule to yield a molecular signature corresponding to the target molecule. The sample is illuminated with a probe light beam, where the probe light beam can scatter radiation from the sample. Radiation scattered from the sample is detected, and whether the radiation exhibits the molecular signature is determined. The target molecule is identified in accordance with the determination of whether the radiation exhibits the molecular signature.

Abstract: A method and apparatus for temperature-independent determination of a concentration of a probe gas in a sample over a selected temperature range between a low temperature TL corresponding to a lowest temperature expected or found in the sample and a high temperature TH corresponding to a highest temperature expected or found in the sample. In accordance with the method, a probe temperature function of the probe gas is determined over the temperature range using a first spectroscopic technique. Then, a second spectroscopic technique is selected, a reference gas is identified and a reference temperature function of the reference gas is determined using the second spectroscopic technique over the temperature range. In particular, the reference gas is identified such that a ratio of the probe temperature function and the reference temperature function is substantially constant over the temperature range.

Abstract: Systems and methods for filter based spectrographic analysis are provided that permit rapid analysis of bioanalytes. Systems include devices for illuminating a sample with electromagnetic radiation and capturing radiation emitted from the sample. Emitted radiation can be collected by a plurality of waveguides each associated with a filter for a particular wavelength of radiation. Focusing devices are associated with filters and waveguides in certain embodiments. Radiation captured by waveguides can then be transmitted to a remote detector, which can determine the intensity of radiation for each waveguide. The use of a plurality of filters having different, band pass characteristics can permits the simultaneous detection of a plurality of different wavelengths of radiation emitted by a sample, thereby providing spectrographic information about the sample under study. Systems can include computers for storing acquired spectrographic information, addressable arrays of samples, and information security measures.

Abstract: In order to provide an arrangement for pressure compensation for optical devices, particularly spectrometers or the like optical devices, for compensating pressure differences caused by changes in temperature and air pressure between the internal pressure and the external pressure at a housing of an optical device enclosing optical units, which arrangement prevents a contamination of optical functional surfaces of the optical units of the optical device and ensures a constant pressure balance between the interior space and the external surroundings of the housing of an optical device with its optical units while economizing on manufacturing costs, it is proposed that the arrangement for pressure compensation comprises at least one pressure compensating element which is constructed on both sides so as to be permeable to air and which is arranged in a housing opening of the housing wall of the optical device enclosing the optical units.

Abstract: An optical system deviation estimating apparatus includes an erected/inverted attitude setting means 9 for changing dispositional attitude of an optical system under test, a non-interferometric type wavefront measuring means 10 for measuring wavefronts at the attitudes as set up without resorting to interference phenomenon of light, a polynomial approximation means 15 for expanding measured wavefront values determined by the non-interferometric type wavefront measuring means 10 to a polynomial, an averaging arithmetic means 11 for averaging the measured values derived from output of the non-interferometric type wavefront measuring means 10 or alternatively arithmetic values derived from output of the polynomial approximation means 15, and a polynomial specific coefficient extraction arithmetic means 16 for extracting specific coefficient values of the polynomial.

Abstract: A tunable optical filter for simulating the waveband spectrums of selected substances. The filter includes an optical waveguide with a core material for transmitting light energy and a nominal core refractive index for the core material. Predetermined periodic variations are formed in the core material of the optical waveguide between the input and output ends that alter the core refractive index of the waveguide at the location of the periodic variations. Depending upon the periodic variations, the waveguide produces a predetermined reference waveband spectrum output that matches the waveband spectrum of a selected substance. A modulator is coupled to the waveguide to selectively modulate the periodic variations to intermittently shift the reference waveband spectrum output to fine tune the filter and reduce signal noise.

Abstract: A device determines and subsequently corrects the dispersive influence on a measurement made according to the principles of phase or pulse modulation along a visible range. The device has an element for emission of electro-magnetic radiation with two carrier wavelengths in the border regions of the visible spectrum. After passing through a volume of atmosphere to be probed, and reflection, the radiation is received and the dispersive influence, for instance, on the distance measurements, is calculated and corrected.

Abstract: An angularly multiplexed store contains filters derived from prior examination of input image reference samples, a spectrum analyzer produces spectral data representing the frequency spectrum of the input image under examination, a computer produces an encoded map of the spectral data representing the input image frequency spectrum. The encoded map is transformed, inputted into the store while close match spectral correlation light beams emerge from the multiplexed store, each having an emerging angle associated with that filter within the multiplexed store producing a close match with the first transform. An array of light beam detectors and a display present images having colors that indicate the nature of the input image such as a type of cancer. An associated memory-retro-reflector arrangement displays characters naming the materials making up the input image such as types of abnormal tissue.

Abstract: A topocompositional image of an inanimate object or surface can be generated that comprises data that relates to a topographical image, data that relates to a chemical compositional image, and combining such topographical and chemical compositional data to form a composite image. Conceptually a laser or other suitable imaging source that functions to induce both topographical and chemical compositional data directs a laser beam into a scanning optics source. The scanning optics source directs an active beam to a point on a surface. A sensor subsequently collects data and information from the surface by receiving the reflection or deflection of the active beams in order to provide position, chemical compositional and topographical data that represent the interaction of the active beam with a point. Position data, chemical compositional data and topographical data are transmitted along individual feeds to a data analysis component where they are analyzed and combined in order to generate a composite image.

Abstract: A method for identifying one or a small number of molecules, especially in a dilution of ≦1 &mgr;M, using laser excited FCS with measuring times ≦500 ms and short diffusion paths of the molecules to be analyzed, wherein the measurement is performed in small volume units of preferably ≦10−14 l, by determining material-specific parameters which are determined by luminescence measurements of molecules to be examined.

Abstract: A top surface of a wafer is provided with an n-type source region, an n-type drain region, and an n-type semiconductor region. Dry etching using a plasma is performed with respect to an interlayer insulating film deposited on the wafer to form openings reaching the respective regions, followed by light etching for removing a damaged layer. In this case, exciting light is supplied intermittently to the n-type semiconductor region. The progression of the removal of the damaged layer and the stage of development of a newly damaged layer are sensed by monitoring the change rate of the intensity of reflected probe light in the presence and absence of the exciting light, resulting in the formation of a semiconductor device having low and equal contact resistance. In-line control using optical evaluation enables the implementation of semiconductor devices with excellent and consistent properties.

Abstract: Spectrographic instruments at remote site locations measure near infrared absorbance spectra for unknown materials and transmit the absorbance spectra to a central web site where, the measurements are standardized to be the same as if they were measured by a master instrument. The standardized absorbance spectra are then compared with a library of spectra stored at the central web site to select a subset of absorbance spectra from the library which most closely correlate with the absorbance spectrum received from the remote sites. The library spectra are obtained from measurements on materials, the measurable characteristics of which are known. From the selected subset, equations are developed relating the measurable characteristics of the unknown material to the absorbance spectra and from these equations, the measurable characteristics of the unknown materials are determined. The results of this analysis are then transmitted back to the remote site locations electronically.

Abstract: The present invention relates to an apparatus for detecting tumorous tissue comprising at least one excitation light source 12, which first excitation light source 12 emits a first excitation light 34 of a wavelength of between 300 nm and 314 nm and includes at least one optical fiber 14 for guiding said first excitation light 34 to an object field 18 of the tissue 16 to be examined, and at least one lens 24 for projecting an auto-fluorescence signal and/or a remission signal 20 of said tissue 16, generated by means of said first excitation light 34, to a CCD or ICCD chip of a camera 22, as well as a data processing system 28 for processing the signals transmitted by said camera 22, said lens 24 being capable of processing UV light and being designed such that at least two images 48, 50 from different spectral regions of said fluorescent object field 18 are generated and projected to the CCD or ICCD chip, of which at least one image 48, 50 represents the UV range and another, different wavelength range of sai

Abstract: A system and method for classifying tissue by application of discriminant analysis to spectral data. Spectra are recorded as amplitudes at a series of discrete wavelengths. Pluralities of reference spectra are recorded for specimens having known conditions. The reference spectra are subjected to discriminant analysis to determine wavelength regions of interest for the analysis. A plurality of amplitudes are selected for the analysis, and are plotted in an N-dimensional space. For each plurality of reference spectra corresponding to a specific known condition, a characteristic point is determined and plotted, the characteristic point representative of the known condition. A test spectrum is recorded from a test specimen, and the plurality of amplitudes corresponding in wavelength to the wavelength regions of interest are selected. A characteristic point in N-dimensional space is determined for the test spectrum.

Abstract: A device and a method enable the rapid, quantitative evaluation of a large collection of ligands for binding affinity with a certain immobilized receptor, the improvements being that binding pan be detected without the need for a label and that binding is carried out in solution phase at a high rate. The instrument has at least two embodiments, one is based on a sensitive absorption photometer and the other on a sensitive light scatter photometer operating at a specific resonance wavelength, &lgr;R, of small, metallic, colloidal particles. The resonance is present in small particles having a complex refractive index with real part n(&lgr;) approaching 0 and imaginary part k(&lgr;) approaching 2 simultaneously at a specific wavelength &lgr;R. The particles are substantially spherical and substantially smaller than &lgr;R. The receptor is immobilized on a suspension of such particles and ligand binding is detected by a change in optical absorption or light scatter at the resonance wavelength.

Abstract: Disclosed is a method for obtaining feedback to drive a servo system for aligning and maintaining alignment in optical systems that bring light to an in vivo skin sample, for adjusting the focus of the optical system, and for adjusting the net depth of focus of the optical system within the in vivo system under characterization. The invention additionally provides a method for obtaining feedback to drive a servo system for aligning and maintaining alignment in optical systems that collect light from an in vivo skin sample, and for adjusting the focus of the optical system. These methods comprise adjusting the angle of incidence of electromagnetic radiation and/or providing a shielding lens to block scattered incident light, or otherwise limiting the field of view of the Raman scattered radiation collection system to exclude optical surfaces of the excitation delivery portion of the optical system. Also provided is a method for identifying spectroscopic depth markers in tissues.

Abstract: A method and system for laser surgery produces controlled laser pulses and simultaneously verifies that a sequence of pulses of prescribed energy are being delivered to the patient. A photo detector receives a predetermined portion of the energy of each treatment pulse. A separate monitoring computer compares an output signal from the photo detector corresponding to each treatment laser pulse with a reference value for that type of pulse obtained in a calibration sequence. Implementation in an ophthalmic laser surgery system is also disclosed.

Abstract: A method of calculating optical frequency spectrum for use in an optical-spectrum measuring apparatus for measuring optical spectrum characteristics of a light source. In the method, a bandwidth storage section stores a characteristic of a bandwidth of passed wavelengths with respect to a measuring wavelength of a spectrometer. A CPU obtains a bandwidth of wavelengths with respect to each measuring point in accordance with the stored bandwidth of wavelengths. Then, measured intensities of light at the measuring points are used to add measured values across the measured value in a required range of bandwidth of optical frequencies. Moreover, correction is performed in accordance with a ratio of the bandwidth of wavelengths at each of the measuring points and the intervals of wavelength at the measuring points. Thus, an intensity of light at each of the measuring points is obtained.

Abstract: The present invention provides a hyperspectral imaging apparatus and methods for employing such an apparatus for multi-dye/base detection of a nucleic acid molecule coupled to a solid surface.

Abstract: A method and apparatus for counteracting adverse drug events (ADE's) which are caused by the administration of intravenous medications of incorrect types, concentrations or dosages, and which may result in morbidity and even mortality to recipient patients. A container, preferably in the nature of a transparent plastic bag employed for intravenous administrations, contains the requisite infusion at a prescribed volume, adding a specified amount of prescribed medication possessing a predefined amount of a coloring material, such as a vegetable dye, to the volume of infusion material or liquid in order to form a prescribed concentration of medication, with the coloring material defining a specific type of medication. Analyzing of the concentration of medication is effected through the utilization of spectrophotometric equipment, and to resultingly obtain a real medication concentration value.

Abstract: The invention uses a resonance Raman spectrometer 1 for achieving the identification and quantitation of analytes including biomolecules, organic and inorganic molecules. According to the present invention, a) a sample 2 is deuterated 3 with D20 for facilitating identification and quantitation of analytes 4 of said sample, b) a monochromatic light 6 illuminates sample 2 of analytes 4 for producing Raman sample light 12 and rejecting Rayleigh light 14, c) the Raman sample light 12 is passed through a depolarizer 19 for producing randomized polarization components 20, d) a Raman sample spectrum 22 is generated, calibrated with respect to an absolute differential Raman cross-section standard in response to said randomized polarization components 22, e) the Raman sample spectrum 22 is provided to a spectral analyzer 24 for identification 26 and/or quantitation 28 of the analytes 4.

Abstract: A physical camera is modeled to render an image in a computer graphics system. When given the manufacturer's specifications of the physical camera's lenses, including the dimensions and indices of refraction of its lenses, stops, and shutter characteristics, the location of the film surface relative to the lens system, and the orientation of the camera within the scene, the invention accurately and efficiently mimics the physical principles of image formation creating an image which approximates an image produced by the physical camera. The procedure comprises four main elements: (1) the geometric relationships between the lens system, object, and film plane are modeled by precise placement and movement of lens elements, (2) image geometry is computed by using principles of geometric optics, (3) an exit pupil is calculated in order to define a region for efficiently sampling rays, (4) the image irradiance, or exposure at a pixel, is computed according to radiometric principles.

Abstract: A digital image processing system reduces errors in the parameters of a sensor geometry model, through which points in a captured digital image are geolocated to the surface of the earth by means of a `real time` co-registration mechanism that refines the geometry model associated with the working image in a matter of seconds. Using a co-registration mechanism such as that described in the U.S. Pat. No. 5,550,937, the system co-registers the reduced accuracy working digital image with a reference image, geographical spatial locations of respective pixels of which have been previously determined with a high degree of accuracy. The imagery co-registration operator adjusts the respective geometry models associated with its input images, in accordance with differences in cross-correlations of the respectively different spatial resolution versions of the two images, so as to bring the respective images into effective co-registration on image registration surface.

Abstract: A method of collecting optical energy which allows the use of short focal length mirrors of relatively small physical size and in particular the use of the standard collecting mirrors usually employed in such spectrophotometers, for a wide range of sample sizes and collection configurations. A relatively large diameter internal surface, which in many cases can be incorporated into the design of other elements in the sampling system, is used. A physically large cell is interfaced to a radiation collector, such as a mirror. In this case the nut sealing the end window on the cell is internally coated (with gold in the preferred embodiment) to constitute a surface and this acts as a transfer mechanism for the backs-scattered radiation from sample. A solid collecting angle of 60 degrees (in this example) can be so obtained to transfer the energy to the standard radiation collector, such as a collecting mirror, of the spectrophotometer.