Abstract: A method for testing a structure using laser ultrasound includes steps of: (1) directing positioning light on a surface of the structure; (2) determining a spatial location and a spatial orientation of the surface from an evaluation of the positioning light reflected back from the surface; (3) directing pump light onto the surface to generate ultrasonic waves in the structure; (4) selectively locating a probe-light focal point of probe light on the surface, based on the spatial location determined for the surface; (5) selectively angularly orienting the probe light normal to the surface, based on the spatial orientation determined for the surface; and (6) directing the probe light onto the surface to detect a response to the ultrasonic waves.

Abstract: In accordance with certain embodiments, a smoke detector determines the presence of smoke particles outside its housing based on measurements of light detected at different wavelengths and corrected based on an ambient light level.

Abstract: An interface including a panel (100) defining at least one edge (114, 116, 118, 120) at least one detector (102) arranged along the at least one edge (114) of the panel, an electromagnetic radiation beam emitter (152) operative to direct at least one beam of electromagnetic radiation onto the panel (100) from a variable distance and at a variable angle (158), the panel being operative to transmit electromagnetic radiation from the at least one beam impinging thereon to the at least one edge (114) thereof, for detection by the at least one detector, the panel being operative to generally attenuate the electromagnetic radiation passing therethrough to the at least one edge as a function of the distance traveled by the electromagnetic radiation through the panel (100), whereby the at least one detector (102) is operative to provide at least one output which can be used to determine the variable distance and the variable angle.

Abstract: In accordance with certain embodiments, a smoke detector determines the presence of smoke particles outside its housing based on measurements of light detected at different wavelengths and corrected based on an ambient light level.

Abstract: Apparatus, systems, methods, and related computer program products for handling temperature variation with optoelectronic components of a hazard detection system are described herein. A power characteristic of an optoelectronic component of the hazard detection system may be used to determine a temperature of an environment of the hazard detection system. A power characteristic of an optoelectronic component of the hazard detection system may be used to determine a smoke condition of an environment of the hazard detection system. Optoelectronic components of the hazard detection system may be optically coupled to determine a smoke condition of an environment of the hazard detection system. Multiple optoelectronics of the hazard detection system may be operative to detect forward scatter and back scatter of one or more types of light to determine a characteristic of a hazard particle.

Abstract: A particle sensor includes: a detecting area into which a gas including particles is introduced; a light-projecting element; a light-receiving element which receives scattered light of the light reflected by the particles in the gas in the detecting area; a heater which heats the gas; a reflector which directs the scattered light to the light-receiving element, wherein the reflector includes: a first ellipsoidal portion having an inner surface shape that defines a portion of a surface of revolution of a spheroid; and a first spherical portion having an inner surface shape that defines a portion of a spherical surface of a sphere, wherein the first ellipsoidal portion has one focus of the spheroid located in the detecting area and the other focus of the spheroid located at or proximate to the light-receiving element, and the first spherical portion has a center of the sphere located in the detecting area.

Abstract: A transportable goniospectrometer with a constant observation center for a radiometric measurement of the reflection of a natural surface includes a spectrometer having an optical unit and a sensor. A main pillar has a lower and an upper pillar end. An arc has a fixed and a free arc end. A slide is disposed displaceably and fixably along the arc. The slide carries the optical unit orientated towards the observation center. A cantilever has a fixed cantilever end connected to the upper pillar end via a screw connection, and a free cantilever end which has a suspension that is rotatable and fixable about a vertical axis. The suspension is connected to the sensor and to the fixed arc end of the arc. The suspension is configured to position the arc at a distance of the arc radius of the arc above the natural surface.

Abstract: Endotoxin detection systems and corresponding detection methods integrate an optical path detection system based on a conventional laser particle size detector and detect the particle size distribution characteristics of the endotoxin colloidal particles in an aqueous solution by using laser light scattering. One system calculates the concentration of the endotoxin by fitting the correlation of the astigmatism with the concentration of the endotoxin based on scattering intensity of the endotoxin particles at more than three different angles via a quantitative operator.

Abstract: An optical system includes a radiation source, a radiation-illuminating device, and a radiation-collecting device. The radiation source is configured to generate radiation. The radiation-illuminating device is optically coupled to the radiation source and configured to direct and focus the radiation obliquely with respect to an optical axis thereof onto a sample. The radiation-collecting device is configured to collect back-scattered radiation scattered from the sample and spatially separated from noise radiation. Associated apparatus and method are also described.

Abstract: A method is described for determining the size of a transparent particle (2), wherein the particle (2) is illuminated with light from a light source (6), wherein using a radiation detector (7) a time-resolved intensity curve of light from the light source (6) scattered on the particle (2) is measured at a preselectable scattering angle ?s, wherein characteristic scattered light peaks are determined in the intensity curve, and wherein the size of the particle (2) is determined on the basis of the time difference between two scattered light peaks, characterized in that, with the help of two radiation detectors (7) or light sources (6), a first and a second time-resolved intensity curve of scattered light, scattered on the particle (2) in the forward direction, are measured; a transmission peak (12) and a reflection peak (11) are determined from the first intensity curve and from the second intensity curve; a first time difference between the transmission peaks (12) is determined, and a second time difference be

Abstract: A method and system for controlled fractionation of particles. A sample having a plurality of particles of different size distributions. A uniform array for the preparing of optical traps having a selected array lattice constant. The plurality of particles for inputting the plurality of particles to the uniform array of optical traps at a driving direction angle ? and the plurality of particles separating along different directions ?v based on variable particle attributes.

Abstract: An analyzer comprises: a sample stream forming section for forming a sample stream including particles; a light source; an optical fiber bundle formed by a plurality of optical fibers, into which light from the light source enters, and which emits light onto the sample stream; and an imaging device for imaging a particle in the irradiated sample stream.

Abstract: An apparatus and method for measuring optical forces acting on a trapped particle. In one implementation the apparatus and method are adaptable for use in the optical train of an optical microscope that is configured to trap, with a single light beam, a particle suspended in a suspension medium between an entry cover and an exit cover of a chamber positioned on or within the microscope. The apparatus and method involves the use of a single collection lens system having a numerical aperture designed to be greater than or equal to an index of refraction index of the suspension medium intended to suspend the particle in the chamber which is placeable at or near the exit cover of the chamber of the microscope.

Abstract: A light detection value determines concentration of a target component, without using a laser emitter of high laser intensity or a large light collector. By changing orientation of the laser emitter about a horizontal axis, or height of the laser emitter, a laser irradiation position on a ground or water surface is switched between first and second irradiation positions. A photodetector detects first scattered light scattering from the first laser beam at the first irradiation position, second scattered light scattering from the second laser beam at the first irradiation position, third scattered light scattering from the first laser beam at the second irradiation position, and fourth scattered light scattering from the second laser beam at the second irradiation position. A concentration calculator calculates concentration of a target component between the first and second irradiation positions, based on detection values of the first, second, third, and fourth scattered light.

Abstract: A light guiding device is operable to receive incident light emitted by a light source through a capture surface. The received light exits the light guiding device through an exit surface provided adjacent to and aligned with an aperture of light receiver. In this manner, light from the light source can be inserted into the receiver where it may be combined with additional incident light captured by the receiver. The light source might be a projector and the light projected may correspond to operational data relating to the operation of the receiver or images corresponding to data captured by a further receiver device operating with a different form of sensor or in a different region of the spectrum. In order to improve the composite image observed by a user of the light receiving device, the operation of the light source can be controlled to vary the intensity of the light emitted. In one example, this variation can be in response to the ambient light level, as sensed by a suitable sensor.

Abstract: A fluid characterization measuring instrument comprises a sample vessel (14) for a bulk complex sample fluid having a capacity that is substantially larger than a domain size of the complex sample fluid and that is sufficiently large to cause bulk scattering effects to substantially exceed surface effects for the complex fluid sample, a coherent light source (12) positioned to illuminate the bulk complex sample fluid in the sample vessel and a first fibre (16) having a first end positioned to receive backscattered light from the sample after it has interacted with the sample. The first fibre is positioned close enough to an optical axis of the coherent light source and to the sample vessel to substantially decrease a contribution of multiply scattered light in the backscattered light.

Abstract: A method for detecting and counting particles suspended in fluids, such as bacteria suspended in urine, utilizing dynamic features of the suspended particles and employing light scattering measurements. The disclosed method is suitable for determining the susceptibility of bacteria to antibiotics. A cuvette for detecting bacteria in fluids, which is especially suited for the light scattering measurements, is provided.

Abstract: A method for detecting and identifying a particle in a liquid, the system comprises controlling the provisioning of a water sample using a computer controlled metering pump; mixing the water sample with particle free filtered water to provide a diluted water sample when required; at the end of a measurement interval, determining a Total Counts Per Minute (TCPM) for the diluted water sample; determining an additional counts per minute from the sample (SCPM) for the diluted water sample; if the SCPM is greater then a Lower Optimum count Rate (LOCR) and less than a Upper Optimum Count Rate (UOCR), then setting a dilution ratio (DR); and correcting an events classification based on the DR.

Abstract: Measuring and tracking velocity of individual aerosol particles in a bio-threat detection system are accomplished using a single beam laser source in combination with a birefringent crystal that splits the laser beam into two beams having orthogonal polarization. Scattered light is collected with an elliptical reflector and directed into two detection channels, sampling total elastic scatter in the first channel and sampling polarized elastic scatter in the second channel. The difference in intensity of the scattered light in the polarized channel is used to identify the position of the particles. By taking the ratio of signal output from the polarized detector to the total scatter detector, a threshold level can be established to determine the presence of particles traversing the two beams. The particles are time stamped as they traverse the two beams and the time difference between the pulses can be used to measure the velocity of the particles.

Abstract: Described herein is a particle detection system capable of spatially resolving the interaction of particles with a beam of electromagnetic radiation. Using a specific electromagnetic beam cross sectional shape and orientation, the detection sensitivity of a particle detection system can be improved. Also provided are methods for detecting and sizing particles in a manner that has low background signal and allows for spatially resolving the scattering or emission of electromagnetic radiation from particles.

Abstract: A system (1) for analyzing solid particles in a medium (2), includes illumination elements (3) capable of generating a light field (30) in the medium (2), elements (4) for trapping at least a portion (30?) of the light field (30) generated and arranged in the direction (31) of the light field, and main detection element (5) for detecting the light field (30?) diffused by the solid particles within the medium (2). The main detection element includes a photodetector (52) for the light field (30?) diffused by the solid particles in the medium and a counter (53) for counting these solid particles in this medium, this main detection element being positioned in a direction (51) forming an angle (?) substantially ranging between 10° and 20° with respect to the direction of the light field generated. A method for analyzing solid particles in a medium implementing such an analysis system is described.

Abstract: There is provided an inside observation apparatus of an endoscope and the like which can perform an inside observation for irradiating an illumination light to a minute area of a surface of an object (for example, a living tissue) having a light scattering property and detecting a back-scattered light of the illumination light, can increase a detected light amount by a simply and low cost configuration by making an area of a detection region larger than an illumination region, and can reduce a time necessary to detect an body (for example, a blood vessel) to be observed and detect a region deeper than a conventional region.

Abstract: The determination of wax crystal particle size and population is used to monitor the performance of wax crystallizers used in lubricant oil processing using solvent dewaxing. The wax crystal particle size is monitored using online measurements. The information obtained from on-line monitoring is then used to control crystallization in the dewaxing equipment in order to optimize performance of the dewaxing units.

Abstract: This smoke detector is provided with: a plurality of light emitting devices which emit light beams of mutually different wavelengths; and a plurality of scattered light receiving sections which receive, at a different scattering angle for each light beam of the respective wavelengths, scattered light generated due to the plurality of light beams emitted simultaneously from these light emitting devices impinging on smoke.

Abstract: A measuring device determines the size, size distribution, and/or concentration of nanoscopic particles or hollow spaces in a measuring sample, the degree of opacity of such measuring samples, or the degree of roughness of surfaces by determining the wavelength and scattering angle dependent intensities of a measuring radiation scattered on a measuring sample. The measuring device comprises a retaining device for a measuring sample to be measured, a detector comprising at least one detector inlet, an evaluation unit, and at least two radiation sources that are respectively at a distance from each other and at a distance from the measuring sample. Via the radiation sources, a ray bundle can in each case be emitted in an essentially parallel beam in the direction of the measuring sample. The ray bundles directed onto the measuring sample are aligned or can be aligned in different angles onto the measuring sample.

Abstract: The present invention relates to a method and an apparatus for determining the layer thickness and the refractive index of a sample. It is an object of the present invention to provide a method for determining the layer thickness of a sample (layer) having high light scattering characteristics that allows a fast (real-time process) and cost-effective measurement having a high accuracy.

Abstract: A device (1) for imaging the interior of an optically turbid medium is provided. The device comprises a receptacle (3; 103) structured to accommodate an optically turbid medium for examination and an optically matching medium filling a space between an inner surface (6; 106) of the receptacle (3; 103) and the optically turbid medium. The device comprises at least one light source generating light to be coupled into the receptacle (3; 103) and at least one detector for detecting light emanating from the receptacle (3; 103). A coupling surface (10; 110) optically coupled to the inner surface (6; 106) of the receptacle and a coupling member (11; 111) optically coupled to the light source and the detector are provided.

Abstract: Changes in cell parts are assayed by transforming light scattering data from a detection optimized control ensemble of cells and a detection optimized treatment ensemble sample of cells, where the control sample is from a sample and the treatment sample is from the sample with at least one detection agent added, and where the light scattering data have at least a primary assay specific data density in at least a primary assay specific angular range at least partly within zero to four degrees from an incident light central ray.

Abstract: The present invention relates to a method and a device for determining a concentration of a biological active substance in a sample by the means of an enzyme-linked immunosorbent assay (ELISA). The device comprises a solid support within a tubing for binding an immunosorbent and an inlet for fluids, a detector for detecting radiation due to an activity in the tubing, wherein the tubing is arranged inside a microchip extending substantially in one plane, for conducting the fluids along the plane of the microchip. The tubing forms a reaction cell having a large detection area. The reaction cell of the microchip is arranged perpendicular to the detector. The method comprises the steps of introducing the fluids into the tubing, conducting the fluids through the tubing forming a reaction cell, in which reaction cell the radiation emitting activity takes place, and detecting the light emitted from the reaction cell substantially perpendicular to the plane.

Abstract: The system and method of the present invention relate to characterizing lipoproteins in a sample. The system includes a light source that delivers electromagnetic energy in a predetermined range of wavelengths to the sample and a sensor that senses an intensity spectrum which emerges from the sample when the sample is illuminated by the light source. A processor determines a chemical composition of the sample to determine the presence of lipoprotein particles. The processor then characterizes lipoproteins that are within in the sample by deconvoluting the intensity spectrum into a scattering spectrum and absorption spectrum. The method includes illuminating the sample with electromagnetic energy having a predetermined range of wavelengths and sensing the electromagnetic energy that emerges from the sample. The method further includes transducing the sensed electromagnetic energy which emerges from the sample into an intensity spectrum that determines the types of lipoproteins that are within the sample.

Abstract: The apparatus for deriving at least one property like turbidity or fluorescence of a sample liquid from measuring emitted light emitted from said sample liquid upon irradiation of said sample liquid with a probe light beam comprises a container for containing said sample liquid, said sample liquid forming a sample surface. It furthermore comprises a light source for generating said probe light beam directed, in an angle ?1?0° with respect to a sample surface normal, at said sample surface, and a detector adapted to detecting an intensity of said emitted light emitted through said sample surface out of said sample liquid generally along a first detection axis, said first detection axis forming an angle ?1?0° with a sample surface normal.

Abstract: Physical property determination of a particle or classification of the particle as a function of the physical property by evaluating scattered light profile from a single particle is disclosed. The particle may include chemical structures that vibrate as a function of a physical property of the particle. The physical property may include an absorptive property of the particle or a chemical composition. From a detected scattered light spectrum, at least two anomalous dispersive regions may be identified. The physical property of the particle may be determined as a function of the at least two regions. A system employing the physical property determination can achieve sensitivities useful for low particle density applications such as detection for biological and chemical agents.

Abstract: Apparatus, systems and methods for measurement of B22 values of proteins in aqueous solutions in flow-mode utilize a dual-detector cell are provided. Simultaneous measurement of protein concentration and scattered light intensity is facilitated as the protein elutes from a size-exclusion column. Each data point on the resulting chromatograms is converted to Rayleigh's ratio, R?, and to concentration c, respectively. The B22 value is calculated from the slope of the Debye plot (Kc/R? versus c) generated from a range of the concentrations obtained from these chromatograms for a single protein injection. Measurements may be analyzed using modeling data derived from a predetermined modeling equation to quantify self-association of molecules. The apparatus and method provide a reliable means for determining B22 values for such proteins as lysozyme, chymotrypsinogen, and chymotrypsin in various solution conditions.

Abstract: A method for optical inspection of a surface includes selecting an apodization scheme in response to a characteristic of the surface, and applying an apodizer to apodize a beam of radiation in response to the selected apodization scheme. The apodized beam of radiation is directed to impinge on the surface, whereby a plurality of rays are scattered from the surface, and at least one of the scattered rays is detected, typically in order to detect a defect on the surface.

Abstract: A system having a flow channel for conveying a sample and having fluid light guides for projecting light to the sample target area and collecting light from the sample target area. The system may have fluid light guides on or off the card containing the flow channel. Accurate alignment may be provided by the fluid light guides in lieu of requiring precise alignment for the light source and detectors. The flow channel may be part of a cytometer system.

Abstract: A laser light source emits a first light beam irradiating a solution including target particles and being flowed in a flow cell to generate forward scattered light and orthogonal scattered light therefrom. A light emitting diode emits a second light beam irradiating the solution in the flow cell to generate at least one wavelength of fluorescence therefrom. A first detector is adapted to detect the forward scattered light. A second detector is adapted to detect the orthogonal scattered light. At least one third detector is adapted to detect the at least one fluorescence. A first filter is disposed between the flow cell and the third detector and adapted to eliminate scattered light generated from the target particles by the irradiation of the first light beam.

Abstract: The invention provides systems and methods for detecting aerosols. The systems and methods can be used to detect harmful aerosols, such as, bio-aerosols.

Abstract: Imaging systems may include a light source that directs light towards a scattering medium. A digital imaging system receives transmitted light that is transmitted through the scattering medium substantially without being scattered, and also receives multiple components of scattered light that are passed through the scattering medium after being scattered. The digital imaging system then outputs image intensity information related to the transmitted light and the scattered light. An imaging controller determines a property of the scattering medium, based on spatial correlations related to the image intensity information.

Abstract: Imaging systems may include a light source that directs light towards a scattering medium. A digital imaging system receives transmitted light that is transmitted through the scattering medium substantially without being scattered, and also receives multiple components of scattered light that are passed through the scattering medium after being scattered. The digital imaging system then outputs image intensity information related to the transmitted light and the scattered light. An imaging controller determines a property of the scattering medium, based on spatial correlations related to the image intensity information.

Abstract: Described is a dark-field imaging device for the spatially resolved dark-field imaging of a sample (60), especially a fluorescent sample, comprising, for the illumination of the sample with excitation light, an illumination assembly (20) comprising a grid (22) of individually addressable light sources (24), wherein the lines connecting the light sources and the sample define a region (64) with the optical path of the direct excitation light and a region (66) with the optical path of the specular reflected excitation light, and a detection assembly (30) for the detection, with radiation-receiving elements (34), of the radiation emitted by the sample (60) as an optical response to the illumination, wherein the radiation-receiving elements (34) of the detection assembly (30) are disposed outside the region (64) with the optical path of the direct excitation light and the region (66) with the optical path of the specular reflected excitation light.

Abstract: A method and apparatus is described by which means molecules in suspension may be characterized in terms of the size and mass distributions present. As a sample solution is separated by centrifugal means, it is illuminated at a particular radial distance from the axis of rotation by a fine, preferably monochromatic, light beam. Despite the high resolution of such devices, a key problem associated with most separators based upon use of centrifugal forces is the difficulty in deriving the absolute size and/or molar mass of the separating molecules. By integrating means to detect light scattered, over a range of scattering angles, from samples undergoing centrifugal separation, molecular sizes in the sub-micrometer range may be derived, even in the presence of diffusion. Adding a second light beam at a displaced rotational angle, preferably of an ultraviolet wavelength, that intersects the sample at the same radial region as the first beam permits determination of the molecular concentration at that region.

Abstract: A response period is determined arbitrarily. A satisfactory detecting ability may not be provided because of an artifact or the like contained in a signal. Therefore, an effective detection method and an effective display method of presenting results of detection must be developed. A series of tasks (stimuli) or a selected measurement signal is used as a reference signal, and a phase difference of any other measurement signal from the reference signal is calculated. The synchronousness of the phase of the measurement signal with the phase of the reference signal is numerically expressed. The thus obtained numerical value is statistically processed in order to numerically express a degree of reliability. Thus, a brain activity or a functional connectivity is visualized.

Abstract: Semiconductor processors, sensors, semiconductor processing systems, semiconductor workpiece processing methods, and turbidity monitoring methods are provided. According to one aspect, a semiconductor processor includes a process chamber configured to receive a semiconductor workpiece for processing; a supply connection in fluid communication with the process chamber and configured to supply slurry to the process chamber; and a sensor configured to monitor the turbidity of the slurry. Another aspect provides a semiconductor workpiece processing method including providing a semiconductor process chamber; supplying slurry to the semiconductor process chamber; and monitoring the turbidity of the slurry using a sensor.

Abstract: Apparatus for detecting light scattered by a small particle (e.g., a blood cell) irradiated by a light beam comprises one or more photodetectors and a plurality of optical fibers that serve to optically couple the scattered light and the photodetector(s). To enhance the efficiency of such optical coupling, a portion of each of the optical fibers in the vicinity of its light-collecting end is supported so that its optical axis extends towards the light-scattering source. By this arrangement, scattered light enters each fiber from a direction substantially parallel to the fiber axis. Preferably, the light-collecting ends of the optical fibers are supported on a concave surface and so that the respective optical axes of the fibers converge at a point representing the apparent position of the light-scattering source, taking into account the refractive effects of an optical flow cell through which scattering is detected.

Abstract: A metrology system for characterizing three-dimensional structures and methods for manufacturing and using same. The metrology system includes a measurement system that preferably comprises an energy source and energy detector and that is in communication with a processing system. Under control of the processing system, the metrology system rotates the measurement system relative to a structure while the energy source directs a beam of incident energy toward the structure. The incident energy rebounds from the structure as scattered energy, at least a portion of which propagates toward the energy detector. Due to the relative rotation, the energy detector receives scattered energy from the structure at a plurality of angles, and the measurement system produces data signals therefrom, which data signals are provided to the processing system. The processing system analyzes the data signals to determine whether the structure has any defects, such as yield limiting deviations or other processing defects.

Abstract: A particle sizing method and apparatus of the PIDS type uses randomly polarized radiation to irradiate a particle sample. Portions of the resulting side scattering pattern are decomposed to simultaneously produce, for each decomposed portion, first and second linearly polarized beams of radiation in which the respective planes of polarization of the two beams are mutually perpendicular. Each of the polarized beams is focused onto a photodetector, and the respective photodetector outputs are differentiated to provide PIDS signals that are useful in calculating a particle size distribution for the sample.

Abstract: A method and apparatus are presented for detecting an effect of interactions of electromagnetic radiation with ultrasound radiation at different locations within a region of interest in a scattering medium to thereby enable imaging of said medium. A plurality of sequences of pulses of ultrasound radiation is transmitted towards a plurality of locations, respectively, in said region of interest within an X-Y plane perpendicular to axes of propagation of the ultrasound pulses. Said region of interest is illuminated with incident electromagnetic radiation of at least one wavelength. The phases of the ultrasound radiation or the phases of the electromagnetic radiation components are appropriately controlled in order to be enable identification of the interactions between the electromagnetic and ultrasound radiation that occur at different locations along the Z-axis.

Abstract: According to the present invention, there is provided a light scattering type particle detector, using a semiconductor laser as a light source, for detecting particles contained in sample fluid which defines a flow path, wherein laser light generated from the semiconductor laser is irradiated to irradiate a region of the flow path with a concave mirror and thereby a particle detecting region is defined. According to the present invention, there is also provided a laser oscillator wherein the optical axis of a semiconductor laser for generating pumping laser light has a predetermined angle with respect to the optical axis of a laser medium for irradiating laser light by pumping. Using such a laser oscillator, laser light irradiated from the laser oscillator is condensed to irradiate a region of a flow path defined by sample fluid, and thereby a particle detecting region is defined. Particles contained in the particle detecting region are detected by receiving scattered light with a light receiving portion.

Abstract: Apparatus for detecting light scattered by a small particle (e.g., a blood cell) irradiated by a light beam comprises one or more photodetectors and a plurality of optical fibers that serve to optically couple the scattered light and the photodetector(s). To enhance the efficiency of such optical coupling, a portion of each of the optical fibers in the vicinity of its light-collecting end is supported so that its optical axis extends towards the light-scattering source. By this arrangement, scattered light enters each fiber from a direction substantially parallel to the fiber axis. Preferably, the light-collecting ends of the optical fibers are supported on a concave surface and so that the respective optical axes of the fibers converge at a point representing the apparent position of the light-scattering source, taking into account the refractive effects of an optical flow cell through which scattering is detected.

Abstract: The present invention is a flow cytometry-based hematology system useful in the analysis of biological samples, particularly whole blood or blood-derived samples. The system is capable of determining at least a complete blood count (CBC), a five-part white blood cell differential, and a reticulocyte count from a whole blood sample. The system preferably uses a laser diode that emits a thin beam to illuminate cells in a flow cell and a lensless optical detection system to measure one or more of axial light loss, low-angle forward scattered light, high-angle forward scattered light, right angle scattered light, and time-of-flight measurements produced by the cells. The lensless optical detection system contains no optical components, other than photoreactive elements, and does not include any moving parts. Finally, the system uses a unique system of consumable reagent tubes that act as reaction chambers, mixing chambers, and waste chambers for the blood sample analyses.