Abstract: A confocal microscope system includes a light source configured to form a light beam, a scanning unit, and an objective lens. The scanning unit is in the form of a mechanically driven scanning unit with a controllable scanning trajectory, and is configured to direct the light beam through the scanning trajectory. The objective lens defines a pupil plane and a focal plane. The light beam is directed from the scanning unit to the objective lens. The confocal microscope system is configured for multi-color line-scanning confocal microscopy, and implements multi-color fluorescence imaging without laser excitation crosstalk.

Abstract: A microscope comprises a housing having a receiving portion for receiving at least one biological sample, an optics module comprising several objectives and an illumination system for illuminating at least one biological sample and/or an acquiring system for acquiring light coming from at least one biological sample, wherein the optics module is arranged in an inner space of the housing. The microscope is characterized in that the microscope comprises a replacement system for replacing an objective by one of the other objectives wherein the replacement system is configured to replace the objective by means of moving the optics module relative to the housing and/or by means of moving the housing relative to the optics module.

Abstract: The present invention is to provide a surgical instrument for performing an endoscopy or a laparoscopy. The surgical instrument includes an adaptive element capable of holding an eyepiece of a 2D single channel endoscope and two eyepieces of a Stereo 2 channel 3D laparoscope. The adaptive element is arranged on a camera head cover of the surgical instrument. Further, the adaptive element is a holder having a cavity which allows the eyepieces of the 2D endoscope or Stereo 2 channel 3D laparoscope to fit positively therewith.

Abstract: Embodiments described herein provide for devices and methods of measuring a pitch P of optical device structures and an orientation angle ? of the optical device structures. One embodiment of the system includes an optical arm coupled to an arm actuator. The optical arm includes a light source. The light source emits a light path operable to be diffracted to the stage. The optical arm further includes a first beam splitter and a second beam splitter positioned in the light path. The first beam splitter directs the light path through a first lens and the second beam splitter directs the light path through a first dove prism and a second lens. The optical arm further includes a first detector operable to detect the light path from the first lens and second detector operable to detect the light path from the second lens.

Abstract: Microscopes with objective assembly crash detection and methods of utilizing the same are disclosed herein. For example, a microscope comprises a microscope body, an objective assembly comprising an objective lens, an objective assembly mount configured to separably attach the objective assembly to the microscope body, and an orientation detection circuit configured to indicate when a relative orientation between the microscope body and the objective assembly differs from a predetermined relative orientation.

Abstract: An immersion objective comprises an objective body in which optical components are accommodated, at least one immersion fluid tank and at least one objective-body coupling connection on the objective body. The immersion fluid tank can be supported in a detachable manner via the objective-body coupling connection. At least one pump is supported via the objective body, wherein the pump is arranged in order to convey immersion fluid from the immersion fluid tank to an objective front side. A control electronics component is supported via the objective body and is configured to control the at least one pump.

Abstract: An immersion set for retrofitting an immersion objective comprises at least one immersion fluid tank, at least one pump fluidly connected to the immersion fluid tank, and a control electronics unit configured at least to control the pump. A fastening device is configured to realize the load-bearing fastening of the at least one immersion fluid tank, the at least one pump and the control electronics unit on the immersion objective or on a mounting adapter for the immersion objective.

Abstract: An image detection scanning method for object surface defects and an image detection scanning system thereof are provided. The method includes capturing a test image according to test light, determining whether the test image is normal, generating a coincidence signal if the test image is normal, coinciding the object with the detection position according to the coincidence signal, sequentially moving one of a plurality of areas on a surface of the object to the detection position, providing detection light facing the detection position, the detection light illuminating the detection position with a light incident angle of less than or equal to 90 degrees relative to a normal line of the area located at the detection position, and capturing a detection image of each of the areas sequentially located at the detection position according to the detection light after the object is coincided with the detection position.

Abstract: Provided is a microscope parameter setting method including step of setting a focal point position relative to a specimen, step of adjusting a microscope parameter, step of acquiring an image of the specimen at the set focal point position, step of recognizing the shape of an observation object, steps of displaying the recognized shape of the observation object such that the recognized shape is superimposed on the image, step of determining whether or not the microscope parameter is appropriate, and step of storing the microscope parameter that has been determined to be appropriate, such that the microscope parameter is associated with the focal point position.

Abstract: A method for examining a sample by light sheet microscopy includes illuminating a sample surface located in an illumination plane by a light sheet propagating in the illumination plane. A position of a light sheet focal point of the light sheet in the illumination plane is moved by changing an optical length of a light path of illumination light forming the light sheet. Detection light emanating from the illumination plane is detected.

Abstract: The invention relates to a method, in particular a fluorescence microscopy or endoscope imaging method for intensity normalization, a non-transient computer readable storage medium (101) and a controller (51) for an endoscope (3) or microscope device (5). In the prior art, common observation devices (1) as surgical microscopes, endoscopes (3) or microscopes (5) have the disadvantage that it is ambiguous whether a detected intensity (97) is due to fluorophore (29) concentration or due to optics setting parameters (73). The inventive method overcomes said disadvantages by automatically adjusting at least one exposure control parameter (83) if at least one optics setting parameter (73) is changed.

Abstract: A liquid immersion microscope objective assembly includes an objective, a liquid delivery system for delivering an immersion liquid to a lens of the objective, and a liquid return system for removing liquid from an area around the lens. The objective may be positioned below a sample container. Liquid is delivered to the lens so as to form a bolus between the lens and the sample container. Excess liquid drains into the return system, which may be done via a notch or a V-shaped channel. Additionally, the direction of flow through the delivery system may be reversed, such that liquid may also be removed from the area around the lens via the delivery system. The assembly may also be utilized to remove liquid from the underside of the sample container. The assembly may also include a leak sensor and/or an electrowetting device.

Abstract: A structured illuminating apparatus includes a branching unit branching an exit light flux from a light source into at least two branched light fluxes, an illuminating optical system making the two branched light fluxes to be respectively collected at mutually different positions on a pupil plane of an objective lens and making the two branched light fluxes to be interfered with each other to illuminate a specimen with an interference fringe of the two branched light fluxes, and an adjusting unit adjusting a height from an optical axis of the illuminating optical system to two collecting points formed on the pupil plane of the objective lens by the two branched light fluxes.

Abstract: Handheld optical devices (HOD) such as binoculars, spotting scopes and riflescopes that have an integrated camera wherein the camera, via Bluetooth and/or Wi-Fi, sends an image to a mobile phone, which then processes the image with a third party computer application for real time identification of the object being viewed are disclosed.

Abstract: A microscope illumination device includes a white LED light source and an illumination optical system. The white LED light source includes a substrate, a plurality of LED chips, and a fluorescent substance layer provided to cover the plurality of LED chips, the plurality of LED chips being arrayed on the substrate and being configured to emit excitation light. The illumination optical system includes a field stop and a light diffusion element that is arranged between the white LED light source and the field stop. The microscope illumination device satisfies 0.2<d/p<1??(1) where p is a minimum interval between centers of the plurality of LED chips and d is a size of each of the plurality of LED chips.

Abstract: The invention relates to a microscope (12) having an objective interchange apparatus (22) comprising a holder (21) for receiving a number of objectives (1) at respective holder positions (21.n) and an objective receptacle (11) which is configured for receiving an objective (1) and is arranged in an optical beam path (13) of the microscope (12). The microscope (12) is characterized by an objective delivery device (20) which is configured for transporting in each case a selected objective (1) having an objective retainer (3) between its holder position (21.n), which is delivered to a transfer position (ÜP), and the objective receptacle (11), wherein the objective receptacle (11) remains in the optical beam path (13) during the transport of the objective (1).

Abstract: Provided is a measuring device that can easily perform high-accuracy measurement at low cost. A stage is held on an installation part. A head unit including a light projecting unit and a light receiving unit, and the installation part are fixedly coupled together by a stand. A light shielding mechanism is attached to the stand in order to block ambient light. The light shielding mechanism includes a front cover member that covers a space on the stage from above. Point cloud data representing the three-dimensional shape of the measuring object is generated based on the light reception signal. Designation of a point to be measured in the measuring object is received, and a measurement value at the designated point is calculated based on the obtained point cloud data.

Abstract: Method for imaging regions of a sample using a light source and an optical detection means and at least one device for moving the sample in three dimensions, comprising the following method steps: a) introducing at least one magnetic element into the sample, b) applying a magnetic field by means of the at least one device for moving the sample in three dimensions, the magnetic field interacting with the at least one magnetic element introduced into the sample, c) arranging the region of the sample in a radiation region of the light source and in a detection region of the detection means, d) emitting first light beams from the light source onto the sample, e) generating second light beams by means of the sample, f) recording an image of a region of the sample by capturing a proportion, incident on the detection means from the sample, of the second light beams, g) moving the at least one magnetic element and the sample containing this at least one magnetic element by varying the magnetic field, h) repeating ste

Abstract: A first resin plate includes a phthalocyanine-based pigment as an organic fluorescent material and is used for shading correction in a case in which infrared excitation light with a center wavelength of 770 nm to 800 nm. A second resin plate includes an anthraquinone-based pigment as the organic fluorescent material and is used for shading correction in a case in which red excitation light with a center wavelength of 650 nm to 690 nm. An acquisition unit acquires a reference image obtained by irradiating the resin plates with the infrared excitation light and the red excitation light, respectively. A correction unit performs shading correction for a fluorescence image on the basis of the reference image.

Abstract: An observation assistance device of the present invention includes: an information storage unit that stores sample state information showing a sample state change, a type of sample, a type of fluorescent substance, and an observation condition in fluorescence observation performed on a predetermined sample, and under a predetermined observation condition; an input unit via which inputs the type of sample to be observed and the type of fluorescent substance to be used; an assistance-display generating unit that reads sample state information and an observation condition of fluorescence observation performed on the same type of sample as the type of sample input, using the same type of fluorescent substance as the type of fluorescent substance, and generates an assistance display showing the sample state information in relation to the two or more parameters included in the observation condition; and a display unit that displays the generated assistance display.

Abstract: An indirect contact lens is mechanically coupled to a surgical microscope during ophthalmic surgery, such as vitreoretinal surgery. The indirect contact lens rests on a cornea of an eye of a patient during the surgery but is supported by a surgical microscope attachment having multiple degrees of freedom to accommodate small movements of the eye while remaining aligned to an optical axis of the surgical microscope.

Abstract: A system for holding a sample for a microscope includes a first container with a substantially transparent wall, and a second container formed from a substantially transparent embedding medium molded on a mounting. The second container is configured to enclose the sample and at least a portion of the second container is positioned within the first container. The second container is configured to be rotated at least in part within the first container about a rotational axis and configured to be placed within a detection optical path of an optical detection axis of the microscope.

Abstract: A lens assembly includes a housing defining an interior volume having a substantially linear optical axis extending therethrough. A zoom group is disposed in the housing in an optical path segment on the optical path between a first lens and an aperture mechanism. The zoom group is movable between a plurality of field-of-view (FOV) positions along the optical path segment. An ultra-narrow field-of-view (UNFOV) lens group is movable between a first UNFOV position outside the optical path and a second UNFOV position in the optical path segment. The lens assembly has a plurality of FOV configurations with the UNFOV lens group in the first UNFOV position, and a UNFOV configuration with the UNFOV lens group in the second UNFOV position. The lens assembly accommodates a number of different FOVs within a limited volume with a small number of lenses, and without folding or otherwise redirecting the optical path.

Abstract: A microscope attachment includes a lens apparatus with one or more lenses, a light source, and a sample holder. The sample holder is disposed between the lens apparatus and the light source and is positioned to transmit light from the light source through the sample holder and through the lens apparatus. The lens apparatus is disposed to enlarge an optical area in the sample holder. An attachment mechanism is disposed to connect the microscope attachment to a personal electronic device.

Abstract: A miniaturized microscope provides the combined capability for simultaneous or sequential optogenetic stimulation of light sensitive ion channels with the capability for fluorescence imaging of fluorescent proteins for applications requiring simultaneous optical stimulation and monitoring of cell activity. The microscope includes a dual illumination output coupling for providing illumination to two different regions within the sample and/or two different illumination source inputs and a dual transmission band optical filter to clean two different bands of light that are separately available for optogenetic stimulation and fluorescence imaging.

Abstract: A method for image processing that includes: obtaining a mask of a connected component (CC) from an image; generating a stroke width transform (SWT) image based on the mask; calculating multiple stroke width parameters for the mask based on the SWT image; identifying a hole in the CC of the mask; calculating a stroke width estimate for the hole based on the stroke width values of pixels in the SWT image surrounding the hole; generating a comparison of the stroke width estimate for the hole with a limit based on the multiple stroke width parameters for the mask; and generating a revised mask by filling the hole in response to the comparison.

Abstract: A procedure and a device for terminating the immersion at a microscope having one or more immersion lenses, or both immersion lenses and dry lenses, comprising a protective sheath around the immersion film area for preventing the escape of immersion media and an immersion media supply unit and an immersion media discharge unit connected with the protective sheath. The immersion media is removed in a controlled manner in particular in an automated microscope. Subsequently, the microscopic testing can be continued without the user having to intervene in the device or the process.

Abstract: A microscope apparatus includes a stage and a revolver that holds objective lenses. At least one of the stage and the revolver constitutes an electric movable device that is electrically operated. The microscope apparatus further includes an input device for inputting a command for operating the electric movable device, a manual focusing device for adjusting a relative distance between the stage and the objective lenses, an operation control device for controlling operation of the electric movable device, a state determining device for determining whether the electric movable device is in an operable state or not, and a restriction device for restricting an output of a control signal for commanding the operation to the operation control device when the command for operating the electric movable device is input if the electric movable device is in an inoperable state.

Abstract: An X-ray camera includes a camera housing, an image pickup element which is sensitive at least for X-rays, and an X-ray objective. The X-ray objective lens has a capillary structure and the image pickup element is constructed as a two-dimensional pixilated semiconductor sensor for simultaneous spatial, energy and time resolution.

Abstract: A retaining device is provided for an instrument, including at least one joint having at least two parts, and at least one drive unit associated with the joint designed to move the joint by driving at least one of the parts. The retaining device includes a securing device, which has a locking mechanism associated with the joint and a release unit that can be activated, wherein the locking mechanism interacts with the release unit such that the locking mechanism holds the joint in a locked position as long as the release unit is not activated. The securing device permits motion of the joint out of the particular locked position within a specified range of motion even if the release unit is not activated. A corresponding securing device for a retaining device and to an operating method for the securing device are also provided.

Abstract: A method for correcting the parcentricity in zoom systems. The positional difference in the image between at least two different zoom positions is detected in order to explicitly or implicitly determine the position in the image which remains stationary during zooming. The travel lengths of a means for positioning the object in the object plane required for correcting the zoom-dependent parcentricity error are calculated and provided as control variable for the appropriate positioning of the means for positioning the object in the object plane, such that the target position appears stationary in the image after or during zooming.

Abstract: A microscope apparatus and a microscopic method that uses the microscope apparatus for examining a sample or a specimen, such as but not limited to a tissue sample or a tissue specimen, includes a convex curved distal exit window at a distal end of the microscope apparatus. Due to the presence of the convex curved distal exit window, the microscope apparatus may readily make contact with the sample or the specimen absent trauma to the sample or the specimen. In addition, an index of refraction matched immersion fluid may be used for focusing the microscope apparatus by hydraulic movement of an objective optic lens assembly interior to the distal exit window with respect to the distal exit window. The convex curved distal exit window and index of refraction matched immersion fluid characteristics may be extended to various microscope apparatuses and methods, and in particular medical microscope apparatuses and methods.

Abstract: A plurality of mounting devices are selectively exchanged while keeping the same parfocal distances for objective lenses among these mounting devices. A microscope system includes a microscope main unit and a plurality of attachable/detachable objective lens units that are selectively attached to the microscope main unit. The microscope main unit includes raising mechanism that can move the attached objective lens unit in an optical axis direction. The plurality of objective lens units have a revolver or a nosepiece that can be attached to the microscope main unit and an objective lens that can be mounted on the revolver or the nosepieces in an attachable/detachable manner. Distances from an attachment position in the microscope main unit for the revolver or the nosepiece to focal positions of the objective lenses are set to be mutually equal among the objective lens units.

Abstract: An optical device includes: a focusing optic that focuses a light beam in a focal plane; at least two phase filters for selectively focusing the light beam and effecting a phase shift of the light beam; and a filter wheel supporting the at least two phase filters which are individually introducible along an optical axis of the light beam, where the filter wheel is rotationally adjusted in relation to the optical axis by a stepper motor and linearly adjusted in an r-direction along a plane of the filter wheel by a linear adjustment mechanism.

Abstract: An optical probe system for probing an electronic device includes a sample plate configured to hold a target device comprising an integrated circuit, a temperature control chamber configured to hold a fluid to control the temperature of the target device, a first optical objective system that can collect reflected or emitted light from the integrated circuit in the target device, a rotational stage including ports that can hold the optical objective systems, a vertical translation stage that can move the first optical objective system in a vertical direction substantially perpendicular to the sample plate, and an x-y translation stage that can move the first optical objective system in horizontal directions. A portion of the first optical objective system is moved through the temperature control chamber to allow the first optical objective system to focus at the target device.

Abstract: A microscope includes a light source, a condenser lens disposed on an optical path of the light source to illuminate a specimen, an objective lens disposed on the optical path on an opposite side of the specimen from the condenser lens, a first polarizing plate disposed between the light source and the condenser lens, a compensator disposed between the condenser lens and the first polarizing plate to adjust retardation of light transmitted through the first polarizing plate, a second polarizing plate for transmitting only one-directional polarization component of the light transmitted through the specimen according to a relative positional relationship with the first polarizing plate, a driving unit for changing retardation of the compensator, and a control unit for causing the driving unit to drive the compensator to increase or decrease the retardation within a range including a position where the retardation is zero as a reference.

Abstract: An illuminating system for transmitted-light microscopes has a illuminator unit seated on a bracket. A condenser is mounted on the bracket by way of a condenser holder and is rotatable with respect to the bracket. The condenser has an integrated modulator slider that slidably extends through the condenser. The bracket has an upright portion with a window through which the modulator slider may be fed in the direction facing away from the operator. the bracket may be provided with a stand mount.

Abstract: The present invention relates to a microscope illumination system for switching between a first, confocal and a second, non-confocal microscope illumination mode, the system having an illumination unit that, in order to provide the first illumination mode, includes an illumination source for generating an illumination beam propagating parallel to the optical axis; a scanning mirror for deflecting the illumination beam perpendicular to the optical axis; and a scanning eyepiece and a downstream scanning tube lens for imaging the scanning mirror into the back focal plane of a microscope objective and for expanding the illumination beam, the objective focusing the illumination beam onto a specimen to be examined. In order to provide the second illumination mode, the system has a focusing lens inserted into the path of the illumination beam in such a way that the illumination beam is focused into the back focal plane of the microscope objective.

Abstract: A microscope includes: a first epi-illumination light-source unit to perform fluorescence observation; a second transmitted-illumination light-source unit to perform transmission observation, the second transmitted-illumination light-source unit including a light source provided with a light emitting element that emits excitation light and a fluorescent substance that emits fluorescence upon irradiation with the excitation light; and an incidence limiting section configured to limit an incidence of light on the light source from an outside of the second light source unit during a light-off period of the light emitting element. The incidence limiting section is configured to remove an incidence limitation of the light from the outside while the light emitting element is being lit.

Abstract: An optical imaging system generates an image of an object plane and includes a lens system which, in turn, includes a main objective and a reduction optical unit between the main objective and the object plane. The reduction optical unit includes a first composite element with a positive refractive power and a second composite element with a negative refractive power. The first composite element includes a first and second lens. The second composite element includes a third and fourth lens. An object-side first main plane and an image-side second main plane are defined by the lens system. The optical imaging system defines an observation beam path which is guided through the lens system so that, in each of the first main plane and the second main plane, the observation beam path is at a distance from the optical axis of the lens system.

Abstract: An optical imaging system generates an image of an object plane and includes a lens system which, in turn, includes a main objective and a reduction optical unit ahead of the main objective. The lens system is aligned along an optical axis and the reduction optical unit includes a first lens with a positive refractive power and a second lens with a negative refractive power. An object-side first main plane and an image-side second main plane are defined by the lens system. The optical imaging system defines an observation beam path which is guided through the lens system so that, in the first main plane and in the second main plane, the observation beam path is at a distance (B) from the optical axis. The first lens is of a first material having a first Abbe number and the second lens is of a second material having a second Abbe number, wherein the first Abbe number is greater than the second Abbe number.

Abstract: A modular laser scanning system where the laser scanner and various optical “building blocks” are contained in separate mechanical and optical modules. These modules can be combined to provide flexible systems with unique laser scanning capabilities or combinations of techniques. Additionally, the combining of these modules is achieved through the use of a mechanical and optical coupling standard.

Abstract: The invention relates to an inverted microscope, including a microscope stand having a stand housing. The stand housing has a rear wall, a front wall, and side walls, and an objective turret, a focusing drive, and a rotatable retaining device, which is designed as a reflector turret and which has a plurality of locations for receiving exchangeable optical components. The retaining device protrudes from a side wall of the stand housing so that the optical components can be quickly changed without having to remove the retaining device from the stand housing.

Abstract: The invention relates to an apparatus for transmitted light illumination for light microscopes having changing effective entrance pupil of an objective. The apparatus has a light source for emitting an illuminating light beam, and a holding device for holding a sample to be examined, and at least one diaphragm edge for trimming the illuminating light beam bundle, wherein the diaphragm edge is arranged between the holding device and the light source and extends transversely to an optical axis, wherein a beam path of the illuminating light between the diaphragm edge and a sample held by the holding device is free from beam focussing components.

Abstract: A dual-configuration microscope is provided. The microscope may be converted into an upright configuration or an inverted configuration. The microscope includes a base having a lower portion and an upper portion, the lower portion configured to support the microscope. The microscope further includes a body having a first portion, a second portion, and an intermediate portion extending between the first and second portions. The intermediate portion of the body is rotatably coupled to the upper portion of the base at a rotational coupling. The rotational coupling defines a rotating axis that extends in a longitudinal direction with respect to the microscope. The microscope further includes an objective disposed proximal to the first portion and a light source disposed proximal to the second portion.

Abstract: An image measuring apparatus with an automated control of variable-power nose wheel rotation and 3D displacement includes a base on a work table on a floor, a platform on the base and having first and second moving parts for controlling the platform moving along X-axis and Y-axis respectively, an upright column on the base and a side of the platform and having a third moving part capable of moving along Z-axis, and a measuring lens group disposed on and displaced with the third moving part. The control device includes a control program for controlling the displacement of the first and second moving parts to change the position of the platform, controlling the displacement of the third moving part to change the position of the measuring lens group, and controlling the measuring lens group to switch to an objective microscope to a different magnification to achieve a fully automated measurement.

Abstract: A device for holding filters for a microscope includes a filter wheel (20) rotatable about an axis of rotation (16) and a drive unit (14) for rotating the filter wheel (20). The filter wheel (20) comprises a basic body (44) rotatable about the axis of rotation (16) and at least one segment (46-54) selectively connectable to the basic body. The segment (46-54) comprises at least two housing areas (58, 59) each holding at least one filter (11). Another housing area may provide a transmission range (36, 59) for unfiltered transmission of light. A second rotatable filter wheel may be arranged at a location displaced along the axis of rotation relative to the filter wheel, and may have its own respective transmission range for unfiltered transmission of light, whereby a filter on either filter wheel may be aligned with the transmission range on the other filter wheel.

Abstract: The invention relates to an illuminating device for a microscope with an illumination magazine comprising a plurality of light emitting units. A mechanical illuminator changer can change the light emitting unit currently active in the operative position. A filter magazine having a plurality of filter units is present, wherein a mechanical filter changer for changing the filter unit currently in the active operative position is associated with the filter magazine. At least one mechanical coupling component is provided for cooperation with the filter changer and the illuminator and for uniquely assigning each filter unit to a specific light emitting unit.

Abstract: A microscope system includes an accommodation unit, a stage, an optical system, an image pickup unit, a movement mechanism, a control unit, an image processing unit, and a display unit. The accommodation unit is capable of accommodating a plurality of specimens. On the stage, each of the specimens loaded from the accommodation unit is placed. The optical system includes a lens for spherical aberration correction. The image pickup unit is capable of capturing a partial image of each of the specimens placed on the stage, via the optical system. The movement mechanism moves the lens for spherical aberration correction along an optical axis. The control unit controls movement of the lens for spherical aberration correction by the movement mechanism and correct spherical aberration. The image processing unit combines the partial images captured by the image pickup unit and generate a composite image. The display unit displays the generated composite image.

Abstract: There is provided a microscope apparatus including: a plurality of objective lenses having different magnifications; an imaging system that receives light, which is generated from a sample and emitted from the objective lens when excitation light is emitted to a sample including a fluorescent material that is activated when irradiated with activation light having a predetermined wavelength and fluoresces to be inactivated when irradiated with excitation light having a different wavelength from the activation light in the activation state and that images the light in a state where an astigmatic difference is given to the image of the sample; and an imaging device that captures the image of the sample from the imaging system. The imaging system includes an astigmatic difference changing device that changes the astigmatic difference according to the depth of focus of the objective lens.