Abstract: The invention relates to a plenoptic ocular device intended to be coupled in an ocular port of an optical instrument configured to generate a real image of a sample on a focal plane situated in a region close to said ocular port, said plenoptic ocular device being configured to capture said real image, generate a set of elemental images and send them to recording means with spatial discretisation which in turn comprises communication means configured to transmit the set of elemental images to external image processing means.

Abstract: A light sheet microscope includes an object slide, and optical illumination and detection systems. The optical illumination system includes an illumination objective configured to illuminate a first object plane that is oblique relative to a slide plane with a light sheet. The optical detection system includes a detection objective and an image sensor device. The image sensor device is configured to define a first image plane which is orthogonal to an optical axis of the detection objective and to define a second image plane which is tilted relative to the first image plane. The detection objective is configured to image a focal plane onto the first image plane and to image a second object plane of the object onto the second image plane, the focal plane being coincident with the first object plane, and the second object plane being parallel to or coincident with the slide plane.

Abstract: The present invention relates in general to microscopy systems. In particular, the present invention relates to microscopes rendering digital images of samples, with the capability to digitally control the focus of the microscope system, and the software used to control the operation of the digital microscope system. Further, the present invention relates to a microscope structure that allows for compact and multi-functional use of a microscope, providing for light shielding and control with samples that require specific light wavelength characteristics, such as fluorescence, for detection and imaging. The microscope is adjustable, with a structure that can move along range(s) of motion and degree(s) of freedom to allow for ease of access to samples, shielding of samples, and manipulation of a display apparatus.

Abstract: An auto-focusing system is disclosed. The system includes an illumination source. The system includes an aperture. The system includes a projection mask. The system includes a detector assembly. The system includes a relay system, the relay system being configured to optically couple illumination transmitted through the projection mask to an imaging system. The relay system also being configured to project one or more patterns from the projection mask onto a specimen and transmit an image of the projection mask from the specimen to the detector assembly. The system includes a controller including one or more processors configured to execute a set of program instructions. The program instructions being configured to cause the one or more processors to: receive one or more images of the projection mask from the detector assembly and determine quality of the one or more images of the projection mask.

Abstract: An imaging device may code light, passing through an imaging optical lens arranged in a multi-lens array (MLA), and may transmit the light to a sensing element, and the sensing element may restore an image based on sensed information.

Abstract: A confocal microscope device for scanning a two-dimensional array of illumination beams over a target surface and scanning a corresponding two-dimensional array of emission beams stimulated by the array of illumination beams on to a sensor of an imaging device. The device comprises first scanning optics operable to scan the array of illumination beams over the target surface along a first axis and scan the array of emission beams over the sensor along the first axis.

Abstract: Disclosed is a defect inspection device for determining anomaly of an inspection object. The defect inspection device may include: a lighting system which includes a light source for transmitting light onto the inspection object; and a dynamic diffuser located between the light source and the inspection object and capable of controlling a diffusivity of light transmitted onto the inspection object; and one or more processors for controlling the dynamic diffuser based on characteristics of the inspection object.

Abstract: The invention relates to an optical assembly for scanning excitation radiation and/or manipulation radiation in a laser scanning microscope.

Abstract: Auto sampler rack mounts and fluid vials that can be used with them are described. In some configurations, the rack mount can be configured to spin each fluid vial rotationally to assist in mixing or stirring of fluid in the vial and/or to maintain fluid homogeneity. If desired, the fluid vial may include one or more internal features to assist in the mixing or stirring.

Abstract: Provided is a microscope apparatus including: a microscope section configured to perform magnified observation of a subject's eye while obtaining a red reflex caused by irradiating a fundus of the subject's eye with illuminating light; a holding section configured to hold the microscope section; and a tilting section configured to tilt an illumination optical axis which is an optical axis of an illumination optical system, and an observation optical axis which is an optical axis of an observation optical system in the microscope section, around a tilt reference point in an interior of the subject's eye as a base point, while maintaining a substantially coaxial state between the illumination optical axis and the observation optical axis.

Abstract: Upstream a microscope objective lens, a polarization direction of a light beam is tilted with a first electro-optical deflector between a first polarization direction with which the light beam is deflected by a first polarization beam splitter by a first angle and a second polarization direction with which it is deflected by a second angle. With a second electro-optical deflector, the polarization direction of the light beam is tilted between a third polarization direction with which the light beam is deflected by a second polarization beam splitter by a third angle and a fourth polarization direction with which it is deflected by a fourth angle. By rotating the polarization direction of the light beam by means of the first and second electro-optical deflectors in a coordinated way the light beam is tilted about a fixed point in a pupil of the objective lens.

Abstract: The invention provides an optical super-resolution microscopic imaging system comprising a dichroic beamsplitter for annular parallel light to transmit through; a focusing lens used for converging the annular parallel light transmitted through the dichroic beamsplitter; a confocal pinhole for the annular parallel light after being converged to pass through to filter the annular parallel light; a varifocal lens system for collimating the annular parallel light passing through the confocal pinhole into excited annular parallel light; and a detector for receiving and processing fluorescence emitted by the excited sample, the fluorescence emitted by the excited sample being returned by the same way, and the dichroic beamsplitter separating the fluorescence emitted by the sample from an annular parallel light path and turning the fluorescence to the detector to obtain a super-resolution image of the sample.

Abstract: A microscope objective is disclosed comprising; a lens; a housing; a lens holder that is arranged to couple the lens to the housing; a first conductor and a second conductor, the first conductor and the second conductor extending along a sidewall of the housing, the first conductor and the second conductor being arranged to form at least one loop that is that is disposed about at least a portion of a perimeter of a field of view of the lens.

Abstract: The disclosure relates to a method for scanning microscopy wherein a specimen is scanned simultaneously with a plurality of illumination spots of an excitation light. The light emitted by one specimen location irradiated with one illumination spot is detected independently of the light emitted by another specimen location illuminated with another illumination spot. A microscopic image of the specimen can be compiled from the emitted light detected for the different specimen locations. The method provides that the intensities of the different illumination spots are set independently of one another, and in that the illumination spots are guided over the specimen one after another in a scan line. The disclosure additionally relates to a scanning microscope.

Abstract: A microscope for adaptive sensing may comprise an illumination assembly, an image capture device configured to collect light from a sample illuminated by the assembly, and a processor. The processor may be configured to execute instructions which cause the microscope to capture, using the image capture device, an initial image set of the sample, identify, in response to the initial image set, an attribute of the sample, determine, in response to identifying the attribute, a three-dimensional (3D) process for sensing the sample, and generate, using the determined 3D process, an output image set comprising more than one focal plane. Various other methods, systems, and computer-readable media are also disclosed.

Abstract: A microscope objective for imaging a specimen using a microscope, the microscope objective being designed as an air objective for microscopy without an immersion medium or as an oil immersion objective for microscopy with an oil-based immersion medium or as a water immersion objective for microscopy with a water-based immersion medium. The front lens of the microscope objective is provided with a coating which repels an immersion medium and is lipophobic and hydrophobic if the objective is an air objective, only lipophobic if the objective is a water immersion objective, and only hydrophobic if the objective is an oil immersion objective.

Abstract: An objective optical system (43) includes: a reflection surface (RS1) which reflects light traveling toward a sample (SP); a reflection surface (RS2) which reflects light reflected by the reflection surface (RS1) toward the sample (SP); and a transmission portion (TS) which is disposed on an optical path of light reflected by the reflection surface (RS2), which has a liquid contact surface coming into contact with liquid (WT) interposed between the liquid contact surface and the sample (SP), and of which the liquid contact surface is formed to be substantially orthogonal to the optical path of light reflected by the reflection surface (RS2).

Abstract: An observation device includes: an illumination optical system configured to emit illumination light to above a sample from below; and an objective optical system configured to acquire an image of transmitted light below the sample in a path different from a path for the illumination optical system, the transmitted light resulting from the illumination light that has been emitted from the illumination optical system, that has been reflected above the sample, and that has passed through the sample. The objective optical system includes, in the vicinity of a pupil plane, a pupil modulation element that partially decreases transmittance and that modulates the phase of light, and the illumination optical system includes a light source, and an illumination-region restricting section configured to restrict light from the light source to a particular emission region.

Abstract: An optical lens system using ultraviolet for imaging includes, in order from a magnified side to a minified side, a first lens group of positive refractive power and a second lens group of positive refractive power. The second lens group includes at least one cemented lens and at least one aspheric lens. The optical lens system satisfies the condition of TE(?=400)>94%, where TE(?=400) denotes an overall transmittance of all of the lenses in the optical lens system measured at a wavelength of 400 nm and is equal to a product of respective internal transmittances of all of the lenses measured at a wavelength of 400 nm.

Abstract: An optical apparatus includes a lens part including a first imaging portion forming a first image of an object and a second imaging portion forming a second image of the object, and a filter part including a filter arranged on at least one of optical axes of the first and second imaging portions and configured to make properties of light for forming the first image and light for forming second image different from each other. The first and second images correspond to a common field stop. F numbers of the first and second imaging portions are different from each other.

Abstract: A medical observation device that includes a microscope that images a surgical site, a holding section that holds the microscope, a base section to which the holding section is connected, and an operation section that receives operating input, the operation section being provided at the base section, the holding section being configured as a balance arm provided with a counterweight, when the base section is viewed from an upper side of the medical observation device, the base section having a first area inside a movable range of the counterweight and a second area outside the movable range of the counterweight, the base end of the holding section being connected to the base section at a location in the first area, and the operation section being arranged at another location in the second area.

Abstract: A medical observation system includes: a microscope; a support including a first joint configured to hold the microscope such that the microscope is rotatable about a first axis parallel to an optical axis of the microscope; and a first arm configured to hold the first joint and extend in a direction different from a direction of the optical axis of the microscope; a light guide cable inserted in the support and configured to guide illumination light to the microscope; and a binder configured to bind the light guide cable, wherein at least a part of the light guide cable is arranged along the first axis in a first section, the light guide cable extends in a direction different from the first axis in a second section, and the binder is configured to bind the light guide cable in the second section.

Abstract: Provided is a light detecting apparatus comprising a light emitting section that emits light; a light detecting section that detects light from an observation target irradiated with the light emitted by the light emitting section; a mount section attached to a test subject that includes the observation target; and a holding section that holds the light emitting section and the light detecting section and is detachably attached to the mount section. The holding section holds the light emitting section and the light detecting section in a manner to secure a relative positional relationship between the light emitting section and the light detecting section, and a relative positional relationship between the holding section and the mount section is determined by attaching the holding section to the mount section.

Abstract: A microscope and method for high resolution scanning microscopy of a sample, having an illumination device, an imaging device for the purpose of scanning at least one point or linear spot across the sample and of imaging the point or linear spot into a diffraction-limited, static single image below a reproduction scale in a detection plane. A detector device is used for detecting the single image in the detection plane for various scan positions, with a location accuracy which, taking into account the reproduction scale in at least one dimension/measurement, is at least twice as high as a full width at half maximum of the diffraction-limited single image.

Abstract: A system includes: an objective lens; a first light source to feed first illuminating light through the objective lens and into a flowcell (e.g., with a relatively thin film waveguide) to be installed in the system, the first illuminating light to be fed using a first grating on the flowcell; and a first image sensor to capture imaging light using the objective lens, wherein the first grating is positioned outside a field of view of the first image sensor. Dual-surface imaging can be performed. Flowcells with multiple swaths bounded by gratings can be used. An auto-alignment process can be performed.

Abstract: A method to capture microscopy images from multiple image sensors and to relay them to one or more central processing units with high frame rates can include preprocessing the image data from the image sensors to reduce the sizes of the captured images, before sending the image data to a central processing station for analysis. Using multiple image sensors and an image reduction process, large image frames of over 20 megapixels and up to 1 gigapixel or more can be obtained at high imaging frame rates of 30 or more to up to hundreds or thousands of frames per second.

Abstract: An optical device for detecting the drift of a light beam of a laser machining system includes a beam splitter for obtaining a first light beam along a first optical path and a second light beam along a second optical path. The optical device further includes a focal module positioned at least partially along the first optical path to obtain a focused light beam that is directed towards a first light beam matrix detection means positioned in a focusing plane associated with the focal module. The optical device also includes an afocal module positioned at least partially along the second optical path to obtain a collimated light beam that is directed towards a second light beam matrix detection means.

Abstract: A sensor head is provided and achieves improved measurement accuracy while reducing measurement time. The sensor head includes: a case including a first case section having a lens therein, a second case section having an objective lens therein, and a third case section providing connection between the first case section and the second case section. Inside the third case section, a mirror member for folding light incident thereon from the lens toward the objective lens is disposed, and a hollow tube providing communication between through holes respectively formed in the mirror member and the objective lens is provided.

Abstract: An optical imaging equipment and method. The optical imaging equipment includes an optical microscope, an objective table, a light source module and an objective lens.

Abstract: To acquire an image of a sample. A microscope includes: an illumination optical system that includes a light flux splitter that splits light from a light source into a plurality of light fluxes, and scans a sample in a plurality of directions with interference fringes generated by interference of at least part of the light fluxes split by the light flux splitter; a detection optical system on which light from the sample is incident; a detection device that includes a plurality of detectors that detect the light from the sample via the detection optical system; and an image processor that generates an image using detection results of two or more of the detectors of the detection device.

Abstract: A scanning microscope and method of operation has a scan filter with a repeat pattern of a plurality of rows that is repeated at least across an active area of an entire surface of an area detector. Each row is covered by a bandpass filter or an emission filter for a specific fluorophore or filters of a particular colour for each row. The scanning microscope can be used to obtain one or more of hyperspectral images, multispectral images, RGB images, RGBW images, W images and Single Field Of View images of a specimen using Moving Specimen Image Averaging. A method of obtaining one or more of the images is also described.

Abstract: A high content imaging system and a method of operating the high content imaging system are disclosed. A microscope has a first objective lens and a second objective lens, and an objective lens database has first and second transformation values associated with the first and the second objective lenses, respectively. A microscope controller operates the microscope with the first objective lens to develop first values of acquisition parameters. A configuration module automatically determines second values of the acquisition parameters using the first values of the acquisition parameters, first transformation values associated with the first objective lens, and second transformation values associated with the second objective lens. The microscope controller operates the microscope using the second objective lens and the second values of the acquisition parameters.

Abstract: A microscopic imaging system and a microscopic imaging method. The system includes: an illumination module configured to generate a laser illumination, an LCOS device located in a Fourier plane of the laser illumination and configured to modulate a phase of the laser illumination, a 4-F system configured to adjust a size of a light beam of the laser illumination, an excitation lens group configured to generate a point illumination focused in a sample plane, a detecting lens group configured to capture an image of a PSF of the point illumination, a camera sensor, and a controller configured to synchronously control a change in a phase pattern of the LCOS device and an image capture of the camera sensor.

Abstract: A dual inclined beam line-scanning confocal microscope apparatus, associated method, and applications thereof. An embodied dual-inclined beam line-scanning confocal microscope (2iLS) utilizes dual, parallel excitation beams each having a focused line shape that are scanned over a fluorescent sample. The emitted fluorescence from the sample is spatially filtered and detected by an array detector. 2iLS microscopy provides high resolution, ultrasensitivity, and deep optical sectioning capability. A reduced excitation intensity lowers photobleaching and photodamage.

Abstract: An additively manufactured component is provided. The additively manufactured component includes an additively manufactured first part defining a first trench, an additively manufactured second part defining a second trench and a fiber optic sensor. The additively manufactured first and second parts are additively manufactured together with the first and second trenches corresponding in position such that the additively manufactured first and second parts form an assembled part with a fiber channel cooperatively defined by the first and second trenches. The fiber optic sensor includes a first sensor part embedded in the fiber channel and a second sensor part operably coupled to the first sensor part and extendible at an exterior of the assembled part.

Abstract: An observation apparatus including: a top plate on which a container in which a specimen is accommodated can be placed, and through which illumination light can pass; a light source that emits the illumination light upward from below the specimen; an objective lens that focuses, below the specimen and the top plate, transmitted light which is the illumination light that has passed through the specimen from thereabove and that has passed through the top plate; and a camera that captures the transmitted light, wherein the light source emits the illumination light toward an area above the specimen from outside the objective lens in a radial direction, and the top plate is provided with a mark that specifies a viewing-field area of the camera.

Abstract: A confocal measuring apparatus (1) includes a light source (10), an optical system (30) configured to receive reflected light from a measurement surface, a light guide part (20) into which a plurality of cores including a first core (26) and a second core (28) is built and configured to propagate the reflected light by the plurality of cores, a displacement amount measurement part (40) including a spectroscope (42) configured to separate the reflected light propagated by the first core into each wavelength components and a detector (44) having a plurality of light receiving elements arranged to correspond to a spectral direction by the spectroscope, and a peripheral image measurement part (60) configured to form an image of the reflected light propagated by the second core on the plurality of image pickup elements and to generate a peripheral image with respect to a measurement position of the measurement surface.

Abstract: A protective device for protecting microscope components from contact with a liquid comprises at least one objective protection ring for arrangement around an objective, wherein the objective protection ring comprises an annular contact area for contacting the objective; and a stand protector with a drainage channel for draining a liquid. The objective protection ring comprises a lower ring area located below the contact area and protruding further outwards radially than the contact area in order to form a free space in the inward direction.

Abstract: An image reproducing device includes one or more processors. The processor acquires image group data including a plurality of images and tag information on at least one tag. The images relate to a biological sample along time series. The tag information relates to an operation on the biological sample and is associated with at least part of the images. The processor selects from the plurality of images using the tag information, images to be reproduced along the time series as reproduction selected images. The processor outputs to a display, data relating to the images for the reproduction selected images to be reproduced along time series.

Abstract: An objective-changer apparatus for a microscope system comprising an objective-transfer element with at least one objective holder for holding an objective that has been provided with an adapter, wherein the objective-transfer element moves a selected active objective into position in a transfer position in controlled fashion and an objective axis of the active objective does not coincide with the optical axis of the microscope system in the transfer position. A receiving apparatus is adjustable in the direction of the optical axis of the microscope system and can be brought into contact with the adapter. The receiving apparatus transports the active objective along a transport path in a transport direction orthogonal to the microscope system's optical axis between the transfer position and a work position in line with the microscope system's optical axis. The transport path is shorter than the extent of the objective holder in the transport direction.

Abstract: A method of preparing a stage for use in a slide imaging apparatus including positioning a stage in relation to a flat surface so that the flat surface is positioned in front of the top surfaces of slide support pin bases on the top surface of the stage. The method also includes injecting a fluid pin surfacing material configured to solidify into the hole of each slide support pin base so that at least some of the fluid pin surfacing material exits the hole at the top surface of the slide support pin bases and pushes up against the flat surface. The method also includes removing the flat surface so that a tip of solid pin surfacing material is formed on the top surface of each slide support pin base, thereby providing the stage with a plurality of slide support pins.

Abstract: A microscope assembly can be used during microbiological processes. The microscope assembly includes a lens assembly for magnified imaging of an object range in an imaging plane along an optical path; a sample receiving unit for a sample arranged in the object range; and a camera receiving unit for receiving a camera in a range of the imaging plane the camera adapted to generate a digital image of the sample; where the lens assembly is a ball lens, a halved ball lens, or a lens in the form of a rotational spheroid; and the camera receiving unit is adapted to receive a customary mobile end device equipped with a camera or a camera cooperating with a mobile end device.

Abstract: An arrangement for microscopy, having an illumination optical unit with an illumination objective for illuminating a specimen on a specimen carrier. An optical axis of the illumination objective lies in a plane which includes an illumination angle that differs from zero with the normal of a specimen plane and the illumination is implemented in the plane. A detection optical unit with a detection objective is located in a detection beam path. The optical axis of the detection objective includes a detection angle that differs from zero with the normal of the specimen plane. The illumination objective and/or the detection objective has an illumination correction element. A meniscus lens is located between the specimen carrier and the illumination and detection objectives being arranged both in the illumination beam path and in the detection beam path.

Abstract: An observation apparatus includes: a stage on which a container accommodating a specimen is mounted; a light source generating illumination light emitted in an upward direction from below the specimen on a specimen placement surface; a light-collecting lens disposed parallel to the surface and collecting the light; a diffusion plate disposed between the lens and the surface, parallel to the surface, and diffusing the light collected by the lens; an objective optical system disposed below the stage and collecting light passing through the stage from thereabove; and an image-capturing optical system capturing, below the specimen, transmitted light, which is the light emitted from the source, reflected above the specimen, transmitted through the specimen, and collected by the objective optical system, wherein the source is positioned so that an optical axis thereof is shifted from an optical axis of the lens in a direction away from the image-capturing optical system.

Abstract: With the aim of shortening the entire length and configuring an entire upright microscope compactly even if coded aperture technology and light field technology are applied thereto, a microscope pupil relay optical system is disposed on an image side of a microscope image-forming optical system including an objective lens, and includes, in the following order from an object side, a first lens group that has a positive refractive power and to which a convergent light flux is introduced from the object side; and a second lens group that has a positive refractive power. A lens located closest to the object-side end of the first lens group is disposed on the object side than a primary image-forming plane on which the microscope image-forming optical system forms an object image, and an exit pupil of the objective lens is re-formed at a position optically conjugate with the exit pupil.

Abstract: A user interface for display of multiview image sets captured by slide scanning digital microscopes, including a graphical user interface (GUI) for the display of one or more of multi-level, multi-angle, and multi-region images.

Abstract: An alignment system for a HUD is provided and includes a mirror, a mirror actuator, first and second light sources, an optic assembly, and a control module. The mirror actuator adjusts mirror orientation. The first light source generates a first light beam. The mirror directs the first light beam from the HUD to a combiner to generate a virtual image. The second light source generates a second light beam. The optic assembly directs the second light beam to the combiner to generate a fiducial. The control module: performs an alignment process including, based on received inputs, controlling the mirror actuator to adjust mirror orientation to adjust a position of the virtual image and align a portion of the virtual image, the fiducial and an eye of a viewer; determines a height of the eye based on a mirror angle; and displays another virtual image based on the height.

Abstract: In a microscopic observation unit (10), hologram data is acquired at each measurement position on a cell culture plate (13) while a light-source section (11) and other related sections are gradually moved by a moving section (15). Every time a set of data for one measurement position is acquired, a measurement monitoring image creator (24) creates a thumbnail image by reducing the size of a hologram image which is based on original data (two-dimensional distribution of light intensity). A display processor (25) pastes the create thumbnail image to progressively complete the hologram image of the entire plate to be displayed on a display unit (27). A measurement operator watches the hologram image during the execution of the measurement. When it has been concluded that the ongoing measurement is inappropriate, the operator presses a measurement stop button to immediately discontinue the measurement.

Abstract: The present disclosure concerns a broadband hyperspectral imaging spectrophotometer configured to analyze an object and includes an illumination assembly having a source for emitting a light beam and configured so that the light beam scans line by line the object to be analyzed, a focusing mirror, a first mirror folding, and a planar scanning mirror movable in rotation. The illumination assembly, the focusing mirror, the first folding mirror and the planar mirror are arranged to bring the light beam to the object along a line which will be displaced on the object via the scanning mirror. The imaging spectrophotometer further includes two measuring sensors by a distance between the object and the scanning mirror. The focusing mirror is movable in translation to adapt the imager to the measured distance by the measuring sensors.

Abstract: A microscope includes illumination optics for fluorescence excitation of point light sources of a sample, detection optics and a camera having a sensor. A density of the point light sources is kept low so as to minimize a crossover of point light sources that are behind or close to one another in each image captured by the camera. A means for subdividing a detection aperture into individual sub-apertures is provided in a beam path of the detection optics such that images generated by the individual sub-apertures on the sensor of the camera depict an object volume from different spatial directions.