Abstract: The invention is directed to a device for marking microscopic preparations. It is characterized in that arc-shaped marks can be applied to a preparation in a simple, ergonomic manner by means of writing tips. For this purpose, the writing tips are lowered onto the preparation and the writing movement is carried out by means of an individual operating element.

Abstract: A microscope stage providing improved optical performance. A carriage for supporting an object has a transparent portion for receiving the object and permitting trans-illumination thereof. A base supports the carriage, at least a portion of the base comprising a transparent material to permit illumination of the specimen there through. Bearings disposed between the base and the carriage support the carriage on the base and permit relative movement thereof. The base has a dovetail cross sectional shape with bearings between the top of the carriage and the base and between the sidewalls of the carriage and the base. A cover is coupled to the carriage so as to transfer force thereto without imparting a significant movement thereto. A mechanism connected to the cover for moving the carriage relative to the base is disposed at a position offset from the axis of lateral symmetry of the carriage and base.

Abstract: The invention relates to a microscope slide formed from a transparent glass plate on which a first coating (3) and a second coating (4) contrasting therewith are located aligned one above another in a marking region (1b), the second coating (4) being designed for providing a marking (6) by removing the material of the second coating (4). In order to improve the microscope slide, it is proposed that the two coatings are located on one side (2) of the glass plate (1), the second coating (4) lying over the first coating (3), and wherein at least the surface of the first coating (3) is designed by comparison with the provision of the marking (6) on the second coating (4).

Abstract: A quantitative cell-counting slide includes a plurality of counting chambers juxtapositionally formed in the slide; each counting chamber including a first counting portion consisting of a plurality of miniature grids of which each miniature grid may be formed to have a volume of 0.01 micro-liter for counting any kind of cells, and a second counting portion formed on a platform and consisting of a plurality of HPF (High Power Field) volumetric grids each formed to have a quantitative volume of 0.0083 micro-liter generally equal to one HPF volume adapted for cell counting used in urinary sediment microscopic examination.

Abstract: The contrast of an image produced by epifluorescence microscopy may be increased by placing a high-pass dichroic reflecting film behind the sample. The reflecting film reflects the emission light emitted by the fluorescent tags in the sample back through the objective lens while allowing the shorter wavelength excitation light to pass through the sample holder.

Abstract: The invention relates to a microscope slide formed from a transparent glass plate on which a first coating (3) and a second coating (4) contrasting therewith are located aligned one above another in a marking region (1b), the second coating (4) being designed for providing a marking (6) by removing the material of the second coating (4). In order to improve the microscope slide, it is proposed that the two coatings are located on one side (2) of the glass plate (1), the second coating (4) lying over the first coating (3), and wherein at least the surface of the first coating (3) is designed by comparison with the provision of the marking (6) on the second coating (4).

Abstract: A solid immersion lens device and method of making. A solid immersion lens device is provided having a plurality of solid immersion lenses. The solid immersion lenses are provided in a predetermined pattern and secured so as to cause them to be in a fixed position with respect to each other.

Abstract: A Z-axis stage for use in, e.g., an orthogonal motion microscope stage, includes a carrier plate, an actuator plate, and a base. Three or more upper camming elements with downwardly directed camming surfaces are mounted to the underside of the carrier plate using a semi-kinematic mounting technique. Three or more lower camming elements with upwardly directed camming surfaces are mounted to the top surface of the base using a semi-kinematic mounting technique. The actuator plate includes apertures to accommodate the lower camming elements and the upper and lower camming surfaces of adjacent camming elements are mated. Linear slides are interposed between the mated camming surfaces and the lower camming elements and the base. Movement of the actuator plate in an X-Y reference plane translates into movement of the carrier plate along the Z-axis (i.e., the optical axis) in response to the relative, sliding motion of the mated camming elements.

Abstract: A unitary counting device for cells and other microscopic particles is described in this invention. The invention also relates to the method of producing the counting device and to the method of using the device. The counting device is constructed with a top part, a connection layer, a base part, and a grid of microscopic lines that are built inside a counting chamber to define counting areas. Furthermore, the counting chamber has a sample introduction port and an air escape port. A connection layer bonds the top part and the base along perimeter of the chamber and maintains gap uniformity. The connection layer consists of a polymer film sandwiched by two layers of pressure sensitive adhesive. Grid lines are fabricated using polymerizable solutions, for narrow and thin lines, which enable counting small particles under high magnifications.

Abstract: This invention is a super-resolution optical cover glass slip or mount which can resolve the optical information on a sample surface without diffraction limit. This super-resolution optical cover glass slip or mount is a multi-layered body at least comprising: (a) a substrate of transparent material; (b) a first protective and spacer layer formed on one surface of the substrate, which is made from transparent dielectric material; (c) a nano structure thin film which is capable of causing localized non-linear near-field optical interaction; (d) a second protective and spacer layer formed on the localized non-linear near-field optical interaction layer, which is also made from transparent dielectric material.

Abstract: A solid immersion lens device and method of making. A solid immersion lens device is provided having a plurality of solid immersion lenses. The solid immersion lenses are provided in a predetermined pattern and secured so as to cause them to be in a fixed position with respect to each other.

Abstract: A near field solid immersion lens array, method of using, and making a near field solid immersion lens array. A cover slide is provided having a near field solid optical lens array having least one fiducial mark. The fiducial mark is used by a positioning device for locating the position of each of the lens in the array. A unique identifier may be provided for locating information of the position of each lens in the array that can be used for driving the positioning device.

Abstract: The glue-on tissue mount is a device to hold small and/or irregular-shaped biologic tissue so tissue can be manipulated, cut, trimmed, split or divide for transplant and reconstructive surgery. Biologic tissue is quickly mounted onto an acrylic mount with fast-bonding cyanoacrylate glue. On the mount tissue can be held firmly. The tissue can be cut on the mount like on a cutting board or dissected into layers. The mount can rest on flat surfaces and be used with an operating microscope or held between fingers. The glue-on tissue mount has a spherical acrylic mounting surface 12 or flat acrylic mounting surface 14 upon which the tissue is glued. The size and shape of the tissue determines if a spherical or flat mount is used. The mounting surface is attached to a cylindrical base 10 that is easily held. If the glue adhesion breaks the tissue can be anchored with sutures tied to the eyelets in the hexagonal footing of the base 10.

Abstract: A slide for the microscopic examination of biological fluids, such as urine, blood, spinal fluids, designed to count particles or elements (e.g., corpuscles) present in a predetermined sample. The slide has two plates made of a transparent material, which distinguish on their inside at least one well that is filled, by capillarity, with drops of biological fluid deposited onto the slide itself. Counting chambers are formed on the bottom of at least one well. Each chamber defines a precise volume of biological fluid to be examined. The slide is characterized by the particular and innovative shape and definition of these counting chambers. In particular, the counting chambers have a bottom surface with a roughness differing from the roughness of the boundary defining or demarcating the counting chamber.

Abstract: A slide for use in microscopic examination of biological fluid is constituted by two plates of transparent material, one plate being a bottom and the other plate being a cover, the plates forming wells which are filled by capillarity with the biological fluid to be examined. A plurality of reliefs form a plurality of computation chambers in each well. The reliefs which define each computation chamber sectioned along a plane perpendicular to the bottom of the well have a shaped section along a profile assimilable to a polygon having at least one right angle.

Abstract: An adhesive label 3 for attaching to a microscope slide 1 is disclosed. The label 3 may have one or more grids or arrays 2 provided on it to help an operator to manually spot a microscope slide 1.

Abstract: The system includes a confocal optical system to illuminate an object at an intended detection plane and to focus light from the object onto a pinhole. An opaque obstacle is disposed within the confocal optical system, the obstacle selected to suppress light originating outside the intended detection plane from passing through the pinhole. It is preferred that the obstacle size and location be selected to achieve a desired level of suppression. A design goal is a 100-fold reduction in background with a 10% or less loss of signal.

Abstract: A cover slip material and a method of making and using the same are provided. The material comprises (a) a light transmissible polymeric backing having first and second surfaces; (b) a tack free bonding layer disposed on the first surface of the backing, the bonding layer comprising polymers selected from the group consisting of acrylate, methacrylate, and combinations thereof; and (c) a protective coating disposed on the second surface of the backing. Upon exposure to an environmentally friendly activating solvent such as terpene, d-limonene, esters of coconut oil and aliphatic hydrocarbon blends, the bonding layer acquires tacky properties causing the cover slip to adhere to a specimen previously mounted on a microscope slide.

Abstract: A microscope slide having a well formed therein, wherein the well is filled with culture media. The slide is designed for on-site collection, staining, and viewing of cells in biological fluid and tissue samples, preferably with an epi-fluorescence microscope. This novel slide permits point-of-care screening in a matter of minutes of any biological fluid or tissue sample for presence of infectious agents, after which, the slide can be transported to a central lab for culture and/or definitive identification.

Abstract: A transparent elongated, rectangular glass microscope slide having a specimen observation spot defined by a removable border or frame. The border corresponds to the rectangular perimeter of the glass slide and includes a tab which may be grasped to peel off the border. In use, a cytological GYN PAP smear specimen is placed on the plain glass within the confines of the releasable paper border. The border is then removed after fixing the specimen. The slide also features a frosted end portion for marking identification data. The frosting may include permanent markings such as “NAME” to designate a certain frosted area for marking the desired information such as the patient's name and identification number. A tab is placed on the border end and overlaps a portion of the frosted area, and thus does not protrude outward from the side of the slide.

Abstract: A cassette for retaining a specimen of surgically exposed tissue from a patient in an orientation that facilitates optical sectioning of the tissue by a confocal microscopic or other optical imaging microscope. The cassette includes a base member having a rigid optically transparent window upon which a tissue specimen is situated, a pliable membrane locatable over a substantial portion of the base member including the window, and an upper member, having an aperture therethrough, which can cover the base member to provide an enclosed cavity between the membrane and the window sealing the tissue specimen therein. With the tissue specimen in the enclosed cavity of the cassette, the edges of the tissue specimen may be positioned planar against the window and retained in that position by bonds formed between the membrane and window at multiple points or locations around the tissue specimen.

Abstract: A variable magnification optical system includes at least three optical units which are a first moving optical unit, a fixed optical unit and a second moving optical unit. The three optical units are arranged in that order in a propagation direction of light, and a variation of magnification is effected by a relative movement between the first moving optical unit and the second moving optical unit.

Abstract: The present invention concerns a microscope, in particular a confocal or double confocal scanning microscope, as well as a method for operating a microscope, at least one specimen support unit associated with the specimen being provided, at least one reference specimen of known configuration being provided, and the reference specimen being detectable by light microscopy for calibration, alignment or adjustment of the microscope. With the microscope according to the present invention and the method according to the invention for operating a microscope, drift-related changes can be detected and compensated for. Auxiliary means with which a specimen can easily and reliably be focused are also provided.

Abstract: A tray or holder for tissue specimens, especially of excised tissue, such as biopsied specimens, is used with a confocal imaging system, especially a laser scanning confocal microscope system. The tray may be disposable after imaging of the specimen carried therein or may archive the specimen. A window supports the specimen. Clamps mounted inside the tray restrain the tissue. A compliant bag is mounted outside the tray on one side of a window of the tray on which the specimen is disposed. During imaging the specimen is immersed in a liquid contained in the tray having an index of refraction which closely matches the index of refraction of the tissue. The bag also contains an index matching liquid preferably having the same index as the liquid in the tray. A stabilizing plate is attached to a surface of the bag which faces the window.

Abstract: The slide for the microscopic examination of biological fluids is constituted by two plates of transparent material, one plate being the bottom and the other plate being the cover, the plates forming wells which are filled by capillarity with the biological fluid to be examined. The reliefs which define each computation chamber sectioned along a plane perpendicular to the bottom of the well have a shaped section along a profile assimilable to a polygon having at least one right angle.

Abstract: A micromanipulator having a high-stiffness manipulation probe capable of carrying out a manipulation process in the view range of a microscope without modifying an existing observation optical system. The micromanipulator comprises a manipulation probe protruding from a probe supporting member, which is connectedly supported on a transfer control device by means of a hold member. The manipulation probe can be moved (e.g., translated or rotated) for a desired stroke in a desired direction by driving the transfer control device to move the probe supporting member finely.

Abstract: The present invention relates to the field of microscopic sample analysis, and specifically relates to a device for preparing thin microscopic samples of liquids.

Abstract: A microscope slide having a well formed therein, wherein the well is filled with culture media. The slide is designed for on-site collection, staining, and viewing of cells in biological fluid and tissue samples, preferably with an epi-fluorescence microscope. This novel slide permits point-of-care screening in a matter of minutes of any biological fluid or tissue sample for presence of infectious agents, after which, the slide can be transported to a central lab for culture and/or definitive identification.

Abstract: A flexible plastic microscope slide which can be folded over such that a viewing portion of the slide can be placed against a specimen to obtain a sample directly therefrom without the need of transferring devices, thereby reducing biological hazards. The slide is designed for on-site collection, staining, and viewing of cells in biological fluid and tissue samples, preferably with an epi-fluorescence microscope. This novel slide permits point-of-care screening in a matter of minutes of any biological fluid or tissue sample for presence of infectious agents.

Abstract: A method and system for imaging a small object in or behind a highly scattering medium comprises a laser source for illuminating the object with an ultrashort collimated beam of light and a novel microscope for forming a magnified image of the object using light emergent from the highly scattering medium, the emergent light consisting of a scattered component and a non-scattered component. The novel microscope comprises an objective, an eyepiece and an aperture centered at the back focal plane of the objective. The aperture, which may be of a fixed or variable size and controlled electronically or by a computer serves to spatially filter the scattered light component of the light emergent from the highly scattering medium. The system may also comprise a streak camera or similar time resolving device positioned at the image plane of the microscope for temporally filtering the scattered light component of the light emergent from the highly scattering medium.

Abstract: The invention is directed to a slide holder for receiving one or more microscope slides. The slide holder includes a generally rectangular frame and at least one slot, each of the slots for receiving one slide. Flexible retaining latches and retaining grooves are provided at each of the slots for facilitating the securing of the slides.

Abstract: A microscope slide receptacle system for positionably receiving and releasably holding specimen retaining microscope slides in a generally vertical disposition. The system includes a plurality of hinged first and second frames each frame comprising a generally inverted "U"-shaped changer and having complementary coupling hinges for inter-coupling multiple frames together to form the system. A releasable clip is fastened to the frame structure for engaging and supporting the slides. Each frame has a support hanger along the upper surface to permit coupling to a system support.

Abstract: The present invention is a blood smearing cassette that quickly, efficiently and reliably prepares peripheral blood smears. The cassette holds two glass slides, a spreader slide and a smear slide, at specific angles to each other. The blood smearing cassette allows untrained personnel to make consistent and proper blood smears. The user slips a smear slide into the smear slide receiver, and a spreader slide into the spreader slide receiver. A small amount of blood is placed on the smear slide, which is then pulled up to the spreader slide until the drop of blood is in contact with the spreader slide. The blood is allowed to spread across the bottom of the spreader slide. The smear slide is then pulled back out of the cassette, leaving a thin film of blood on the smear slide. The length and thickness of the film are controlled by varying the speed at which the smear slide is pulled and varying angles between the two slides.

Abstract: An improved receptacle or holder for microscope slides used in scientific, medical or medically related procedures, with the receptacles being configured to positionably receive, hold and index microscope slides either vertically or horizontally by means of an articulated array of individual holders. The design further minimizes slide-to-cradle contact, with the configuration allowing for rapid drainage of liquids from the slide surfaces. The cradle is also configured to facilitate manual grasping of the edge surfaces of slides being held within the cradle.

Abstract: An illumination apparatus with a specimen slide holder, an illumination source, an optical cavity producing multiple reflection of illumination light to a specimen comprising a first and a second reflective surface arranged to achieve multiple reflections of light to a specimen is provided.

Abstract: A system for managing specimens in a clinical laboratory comprises specimens and a computer controlled instrument. Each specimen includes a biological sample and an identifier which is unique to the specimen to which it is applied. The computer controlled instrument includes a reader for automatically logging and verifying the specimen to be analyzed, and a print head for modifying the identifier to indicate whether the specimen has been analyzed and whether the sample includes any abnormalities. The print head is also used to indicate whether the specimen has been reanalyzed. Further, the print head works to print symbols adjacent the locations of the abnormalities in the sample.

Abstract: An erect movable objective lens type optical microscope includes a frame, an observation optical portion having an objective lens, a stage portion, a moving mechanism, and a vibration synchronizing mechanism. The observation optical portion is movably provided on the frame, and the stage portion is fixedly provided on the frame. The stage portion includes a specimen moving stage, and a stationary stage adapted to receive a manipulator for manipulating a specimen provided on the specimen moving stage. The moving mechanism is provided on the frame for moving the observation optical portion and the stage portion relative to each other, and the vibration synchronizing mechanism is provided for synchronizing a vibration system of the stationary stage and a vibration system of the specimen moving stage.

Abstract: The present invention relates to the field of quantitative microspectroscopy, and in particular to an apparatus for determining the exact thickness of an optical microscopic sample at the time an absorbance measurement or other spectroscopic measurements are performed on this sample. This apparatus can be an optical microscope comprising a means for containing a sample wherein said means has first and second optical markers; a means for holding said means for containing a sample; a means for illuminating the sample in said means for containing a sample; a means for automatically focusing on first and second optical markers in said means for containing a sample; and a means for performing a pattern recognition procedure for said optical samples, wherein said means comprises an imaging receiver which is connected to an image processor and a computer.

Abstract: Disclosed are methods for accurately determining the total emission intensity of a single fluorochrome, under imaging conditions, using a digital imaging fluorescence microscopy system. Also disclosed are methods for detecting and localizing probe-target molecule binding. The detection method has sufficient resolution and sensitivity to locate and detect a single target-bound probe.

Abstract: A calcium phosphate based thin film on which bone cells may be cultured to permit evaluation of bone cell functional properties comprises calcium phosphate entities which provide for varying degrees of resorption of the calcium phosphate entities in evaluating the functional properties. The film is sufficiently thin that resorption of the entities can be detected. Such film, as applied to a support, is a very useful analytical component for evaluating such bone cell functional characteristics. An analytical device, which may be used in an analytical kit, can be provided having a plurality of wells with the devices located at the bottom thereof. A process is provided for making the film and especially the film which has a combination of calcium hydroxyapatite with tricalcium phosphate.

Abstract: A slide marker that automatically makes a mark onto a predetermined location of a slide using a dry marking medium. A puncher during a full cycle of movement tamps a punched through portion of the marking medium onto the predetermined location of the slide with near zero contact velocity. The marking medium has a contact surface having a pressure sensitive adhesive such that the mark will remain securely on the predetermined location of the slide. A section of unused marking medium is spooled into a throat of a puncher guide that carries the puncher over the predetermined location on the slide. A controller and sensor automate the process of making a mark at a predetermined location of the puncher by limiting the movement of the puncher to one full cycle of movement when the puncher is over any predetermined location of the slide for marking.

Abstract: A self-staining microscopic slide designed for immediate staining and viewing of cells in biological fluid and tissue samples, preferably with an epi-fluorescence microscope. The pre-prepared microscope slide preferably has a supravital fluorescent stain applied thereon, which is overlaid with a transparent tape or film. During use, the film is peeled back to expose the stain so that a sample can be applied thereon for intermixture therewith. The film is then replaced over the stained sample to act as a cover slip for immediate viewing. Living cells and microorganisms are rendered dramatically visible and cellular dysmorphology can be readily ascertained. The time and cost associated with preparing a fixed and sectioned sample is completely avoided as well as the problem of artifacts and sample fragmentation found in fixed preparations. The slide can include reference standards to facilitate microscope focusing, and to allow measurements of cells and microorganisms.

Abstract: A computerized slide encoder for use with microscope analysis and pathological studies is provided. The slide encoder is attached to the movable microscope stage, whereby x-y direction plane movement and location, is correlated to examination of an identified slide, with information marking and location being directly correspondingly written, at pre-defined times, on computer storage media, during the examination. Subsequent use of the computer-stored information, coupled with the slide encoder, in a slide re-examination, permits independent retrieval of such information and location on the slide. The computer storage media includes digitized vocal transcription of history of the slide made during the original examination. An embodiment of the slide encoder includes a computer mouse pen affixed to the stage of the microscope and a rolling surface, for the mouse pen, being affixed to the movable slide holder.

Abstract: A slide for depositing for observation material under a microscope comprising a mounting surface to receive material for observation and a removable layer on the mounting surface formed with at least one opening in the removable layer to define at least one exposed region on the mounting surface for retaining material to be observed when the removable layer is detached. The exposed region has definite boundaries and any material to be examined remaining on the slide is confined to the exposed region to facilitate examination of the slide by human visual identification or machine vision by making material easier to locate, by limiting material to regions appropriate for best observation (e.g. away from edges of the slide and coverglass), and by limiting the amount of material or area covered by material to permit quick and rigorous observation and analysis. A method for using slides according to the present invention is also disclosed.

Abstract: A computerized specimen encoder for use with microscope analysis and pathological studies. The slide encoder is attached to the movable microscope stage, whereby X-Y plane movement and location, is correlated to examination of a specimen on an identified slide, with information marking and location being directly correspondingly written on computer storage media, during the examination. The information marking is in the form of computer generated indicia which are placed at a computer image location of the slide at predetermined time intervals. Subsequent use of the computer-stored information, coupled with the slide encoder, in a slide re-examination, permits independent retrieval of such information and location on the slide. The encoder device is provided with a grayscale marker which marks in varying shades of gray, ranging from white to black, the time spent by a slide screener on a particular portion of the specimen and the number of times spent viewing a particular portion of the specimen.

Abstract: A microscope slide having on a first side an opaque, scratchable coating located at a marking region of the slide, and on a second opposing side located generally behind the scratchable coating at the marking region, a contrastingly-colored coating. Upon scribing an identification mark into the scratchable coating and thus removing the scratchable coating where scribed, the contrastingly-colored coating on the second side of the slide becomes visible from the first side of the slide in the area scribed.

Abstract: A computerized slide encoder for use with microscope analysis and pathological studies. The slide encoder is attached to the movable microscope stage, whereby x-y direction plane movement and location, is correlated to examination of an identified slide, with information marking and location being directly correspondingly written, at pre-defined times, on computer storage media, during the examination. Subsequent use of the computer-stored information, coupled with the slide encoder, in a slide re-examination, permits independent retrieval of such information and location on the slide. The computer storage media includes digitized vocal transcription of history of the slide made during the original examination. An embodiment of the slide encoder includes a computer mouse pen affixed to the stage of the microscope and a rolling surface, for the mouse pen, being affixed to the movable slide holder.

Abstract: Slide for the microscopic evaluation of liquid specimens comprising a base plate, at least one evaluation chamber and a cover foil. The evaluation chambers have a specimen application zone and an evaluation area. A capillary gap is located between the evaluation zone and the cover foil. An improved slide compared to known devices was achieved by using a foil to cover the evaluation chambers and by spacers which ensure a defined thickness of the capillary gap.

Abstract: A microscope stage which provides for displacements in simultaneous rotation and linear translation or combinations thereof while also providing for optical coupling of an illumination light source to an object being addressed. The stage can generally be a plurality of optically transparent plates in proximity to one another having a grease interface therebetween. The grease allows movement of the plates with respect to each other in any direction parallel to their surfaces. The grease and plates are arranged to allow light to be transmitted therethrough. An object can be illuminated through the stage from a source located with the stage between the object and the source.

Abstract: A microscope slide and read-write system whereby the slide has a pathology specimen thereon and machine readable high density recording media in the form of a magnetic strip, optical reading strip or the like. The read-write system accommodates initial writing of slide and patient identification information on the recording media. The read-write system further embodies elements for operative connection to a microscope system whereby a computer generated representation of the screening history of pathology specimen is recorded and maintained during pathology analysis of the slide showing the mode and parameters of the analysis as well as position related events of interest. The computer generated screening representation is written to the recording media by the read-write system, for constant proximate availability with the slide.