Cell Analysis, Classification, Or Counting Patents (Class 382/133)
  • Patent number: 11456059
    Abstract: Embodiments of a system, method and apparatus incorporate two-stage identification of targets in hyperspectral image analysis. In various embodiments, unmixing is employed that integrates F-test and model averaging approaches. Further, a multi-tier target library process provides an improvement in the spectra that can be used to detect target materials and spectra that can be used for unmixing in identification. Additionally, the hierarchical identification of the present disclosure combines probabilities from model averaging to generate target identifications simultaneously at multiple levels of specificity.
    Type: Grant
    Filed: August 22, 2018
    Date of Patent: September 27, 2022
    Assignee: Geospatial Technology Associates LLC
    Inventor: William F. Basener
  • Patent number: 11455730
    Abstract: An image processing apparatus includes a first extraction unit configured to extract a first target region from an image using a trained classifier, a setting unit configured to set region information to be used in a graph cut segmentation method based on a first extraction result including the first target region, a second extraction unit configured to extract a second target region using the graph cut segmentation method based on the set region information, and a generation unit configured to generate a ground truth image corresponding to the image based on a second extraction result including the second target region.
    Type: Grant
    Filed: March 30, 2020
    Date of Patent: September 27, 2022
    Assignee: CANON KABUSHIKI KAISHA
    Inventors: Daisuke Furukawa, Fukashi Yamazaki, Keita Nakagomi
  • Patent number: 11449972
    Abstract: The present invention concerns identifying matching tissue objects in two sections of a tissue block, as imaged using a microscope, for the purpose of mapping the biomarker-specific staining in one section onto the other section. The invention is useful because, in many workflows, it is not possible to add a sufficient number of different biomarker-specific stains to a single slide. By staining instead multiple slides, and mapping the staining data obtained across the slides, one obtains a data set that is similar to what one would be able to obtain by staining a single slide with all those stains. The invention first identifies a set of obviously correct matches, then propagates from those matches, using a priority queue driven process, to optimally match up all fibers in the two sections. The matching is based on the shape and neighborhood configuration of each tissue object.
    Type: Grant
    Filed: October 14, 2019
    Date of Patent: September 20, 2022
    Assignee: Flagship Biosciences, Inc
    Inventor: Cris L. Luengo Hendriks
  • Patent number: 11443426
    Abstract: Techniques are provided for determining a cell count within a whole slide pathology image. The image is segmented using a global threshold value to define a tissue area. A plurality of patches comprising the tissue area are selected. Stain intensity vectors are determined within the plurality of patches to generate a stain intensity image. The stain intensity image is iteratively segmented to generate a cell mask using a local threshold value that is and gradually reduced after each iteration. A chamfer distance transform is applied to the cell mask to generate a distance map. Cell seeds are determined on the distance map. Cell segments are determined using a watershed transformation, and a whole cell count is calculated for the plurality of patches based on the cell segments. A client device may be configured for real-time cell counting based on the whole cell count.
    Type: Grant
    Filed: January 15, 2019
    Date of Patent: September 13, 2022
    Assignee: NantOmics, LLC
    Inventors: Bing Song, Liudmila A. Beziaeva, Shahrooz Rabizadeh
  • Patent number: 11442018
    Abstract: A system (90) for imaging microscopic samples comprises a light source (31) for exciting fluorescence from at least one sample, a photosensor (40) configured to detect light deflected by a beam splitter (20) from an optical excitation path directed to the sample and output an electrical signal of optical flux to the sample, a camera (30) configured to receive and form an image of fluorescence light emitted from the sample, and a controller (134) comprising an integrator (122) configured to integrate the electrical signal from the photodetector and a comparator configured to compare the integrated output to a predetermined threshold, wherein the controller is configured to control an exposure time of the camera such that each sample receives substantially the same total optical flux of incident light during a duration of camera exposure which is terminated when a predetermined threshold representative of the total optical flux is met.
    Type: Grant
    Filed: January 23, 2019
    Date of Patent: September 13, 2022
    Inventors: Andreas Gfroerer, Frank Keidel, Bernhard Schinwald
  • Patent number: 11442262
    Abstract: An image processor includes an image generator configured to generate corresponding image data corresponding to first microscopic image data obtained under a first observation condition, based on second microscopic image data and third microscopic image data obtained under a second observation condition, and an image output unit configured to output the corresponding image data. The corresponding image data may be image data corresponding to a first focal plane from which the first microscopic image data are obtained, and wherein the second microscopic image data and the third microscopic image data may be image data on a second focal plane and a third focal plane, respectively, which are different from the first focal plane.
    Type: Grant
    Filed: January 16, 2017
    Date of Patent: September 13, 2022
    Assignee: NIKON CORPORATION
    Inventors: Ichiro Sase, Yutaka Sasaki, Takaaki Okamoto, Yuki Terui, Kohki Konishi, Masafumi Mimura, Martin Berger, Petr Gazak, Miroslav Svoboda
  • Patent number: 11436718
    Abstract: An image analysis method for generating data indicating a tumorigenic state of an image of a tissue or a cell. The image analysis method is an image analysis method for analyzing an image of a tissue or a cell using a deep learning algorithm of a neural network structure, analysis data are generated from the analysis target image including a tissue or cell to be analyzed, the analysis data are input to the deep learning algorithm, and data indicating the tumorigenic state of tissues or cells in the analysis target image are generated by the depth learning algorithm.
    Type: Grant
    Filed: November 16, 2018
    Date of Patent: September 6, 2022
    Assignees: NATIONAL CANCER CENTER, SYSMEX CORPORATION
    Inventors: Hiroshi Yoshida, Yosuke Sekiguchi, Kazumi Hakamada, Yuki Aihara, Kohei Yamada, Kanako Masumoto, Krupali Jain
  • Patent number: 11436493
    Abstract: A chromosome recognition method based on deep learning includes the following steps: step 1, obtaining an independent chromosome image; step 2, calculating a manual feature of a chromosome; step 3, performing basic image processing on the chromosome; step 4, building a deep learning model; and step 5, predicting a type of the chromosome based on the deep learning model. By adopting a deep learning method, the chromosome recognition method can be used for recognizing the chromosome type accurately and efficiently. Compared with an existing recognition technology, the chromosome recognition method based on deep learning of the present invention has the advantages that the chromosome karyotype analysis efficiency can be effectively improved, the recognition sequencing time can be shortened, automatic classification and sequencing of chromosomes can be completely with high accuracy.
    Type: Grant
    Filed: June 6, 2019
    Date of Patent: September 6, 2022
    Assignee: Hangzhou Diagens Biotech Co., LTD.
    Inventors: Ning Song, Chaoyu Wu, Weiqi Ma
  • Patent number: 11434463
    Abstract: A system for controlling a motion of a cell culture includes a tray adapted to hold a cell culture, a camera adapted to capture an image of the cell culture, and a device adapted to control a movement of the tray. The system also includes a processor adapted to determine a first movement of the tray, receive from the camera data representing an image of the cell culture, determine a characteristic of the cell culture based on the image data, determine a second movement of the tray based on the characteristic, the second movement being different from the first movement, and cause the tray to move in accordance with the second movement.
    Type: Grant
    Filed: August 31, 2020
    Date of Patent: September 6, 2022
    Assignee: VBC Holdings LLC
    Inventors: Francesco Armani, Giacomo Cattaruzzi, Francesco Curcio, Massimo Moretti, Antonio Sfiligoj, Piero Fissore
  • Patent number: 11427849
    Abstract: Culture medium devices and systems are shown and described. In one embodiment, the device comprises a culture medium adapted for test fluid inoculation without the concerns associated with a spreading step. In particular examples, a printed grid on the outer surface of a culture device is visible on the inner surface for colony counting after a test has been developed. The result is a device that allows for detection, identification, and transportation of various microorganisms without preparation or spreading steps, and more particularly to a culture medium in which a test fluid inoculated thereto diffuses rapidly.
    Type: Grant
    Filed: September 16, 2013
    Date of Patent: August 30, 2022
    Assignee: Charm Sciences, Inc.
    Inventors: Robert S. Salter, Gregory W. Durbin, Stanley E. Charm, Richard T. Skiffington
  • Patent number: 11417096
    Abstract: Methods, systems, and computer program products for classifying a spatial format of a video file. The system includes one or more processors and a memory coupled to the processors. The memory stores data comprising program code that, when executed by the processors, causes the system to allow video sharing platforms to support multiple formats without asking users to manually identify the format of the video.
    Type: Grant
    Filed: May 21, 2020
    Date of Patent: August 16, 2022
    Assignee: Vimeo.com, Inc.
    Inventor: Or Fleisher
  • Patent number: 11416983
    Abstract: A client and server are provided with the same digital image of a slice of a biological material, which has been applied with a staining substance. The server is used for pre-processing the digital image and provides results of the pre-processing to the client in turn of a request. The server is configured to classify each pixel of the digital image, wherein the pixel is classified as stained, The client is configured to determine the region of interest at the digital image, and request data related to the classification of the pixels at the region of interest at the server. The server can provide the classification results related to the classification of the pixels at the region of interest at the digital image to the client.
    Type: Grant
    Filed: August 12, 2016
    Date of Patent: August 16, 2022
    Assignee: KONINKLIJKE PHILIPS N.V.
    Inventor: Christiaan Varekamp
  • Patent number: 11409093
    Abstract: An image processor includes an image generator configured to generate corresponding image data corresponding to first microscopic image data obtained under a first observation condition, based on second microscopic image data and third microscopic image data obtained under a second observation condition, and an image output unit configured to output the corresponding image data. The corresponding image data may be image data corresponding to a first focal plane from which the first microscopic image data are obtained, and wherein the second microscopic image data and the third microscopic image data may be image data on a second focal plane and a third focal plane, respectively, which are different from the first focal plane.
    Type: Grant
    Filed: January 16, 2017
    Date of Patent: August 9, 2022
    Assignee: NIKON CORPORATION
    Inventors: Ichiro Sase, Yutaka Sasaki, Takaaki Okamoto, Yuki Terui, Kohki Konishi, Masafumi Mimura, Martin Berger, Petr Gazak, Miroslav Svoboda
  • Patent number: 11409094
    Abstract: A template-based image analysis system and method are disclosed. A slide is loaded in a high-content image system (HCIS), wherein the slide includes a plurality of reference marks and a plurality of sample locations. An acquired images data store has previously acquired images stored therein. An image acquisition module acquires an image of the slide from the high-content imaging system. A feature identification module develops a binary image of the acquired image, wherein the binary image identifies areas associated with reference marks. A template generation module generates a template in accordance with the previously acquired images and a template alignment module aligns the generate template with the binary image. An offset calculation module determines an offset between the template and the binary image and an image segmentation module determines the coordinates of pixels of the acquired image that are associated with the sample locations in accordance with the calculated offset.
    Type: Grant
    Filed: August 24, 2017
    Date of Patent: August 9, 2022
    Assignee: Molecular Devices, LLC
    Inventor: Paula Karen Gedraitis
  • Patent number: 11405592
    Abstract: Systems and methods are provided for selecting colony locations. Selecting colony locations can include determine a location of a selection tool on a culture plate image, determining a location of a potential source of error on the culture plate image, comparing the location of the selection tool to the location of the potential source of error; and determining an error when the location of the selection tool overlays the location of the potential source of error.
    Type: Grant
    Filed: November 2, 2017
    Date of Patent: August 2, 2022
    Assignee: BECTON, DICKINSON AND COMPANY
    Inventors: Strett Roger Nicolson, Mark Sakowski, Paul Fieni, Mark Larsen, Keri Lynne Jones Aman, Amy Alcott Llanso, Harry Yuheng Chou
  • Patent number: 11398032
    Abstract: [Problem] To evaluate objective cell groups by estimating a mixing ratio of objective cell group without affecting treatment itself and its production process when the objective cell groups include plural kinds of cell groups having different attributes, and to provide an image analysis system and a culture management system capable of accurately perform a quality control and a production control with low cost. [Solution] The cell quality evaluation system 1 detects a feature amount in each cell easily analyzable from images, and estimates a mixing ratio of each of plural kinds of cell groups included in the objective cell groups based on a distribution of the detected feature amount and pre-recorded information of the feature amount. As the feature amount, the embodiment uses a migration speed of each cell, easily distinguishable by analyzing tracking of each cell from plural images in time series.
    Type: Grant
    Filed: July 13, 2017
    Date of Patent: July 26, 2022
    Assignees: DAI NIPPON PRINTING CO., LTD., TERUMO KABUSHIKI KAISHA
    Inventors: Ryoma Bise, Yoshitaka Maeda, Masatoshi Kuroda, Kazumasa Yamaki, Ryohei Takeuchi, Fumiya Ohashi, Kouichirou Yori
  • Patent number: 11379983
    Abstract: An analysis device configured to analyze an attribute of a correlation between responses with respect to a stimulus to a first subcellular component that is a subcellular component of a cell and a second subcellular component different from the first subcellular component, the analysis device including an attribute analysis unit that analyzes the attribute of the correlation between the first subcellular component and the second subcellular component based on a first change in a feature value of the second subcellular component with respect to the stimulus in a state a function of the first subcellular component is suppressed and a second change in a feature value of the second subcellular component with respect to the stimulus in a state the function of the first subcellular component is not suppressed.
    Type: Grant
    Filed: June 23, 2017
    Date of Patent: July 5, 2022
    Assignees: NIKON CORPORATION, THE UNIVERSITY OF TOKYO
    Inventors: Takuro Saigo, Shoko Yamasaki, Masayuki Murata, Fumi Kano, Yoshiyuki Noguchi
  • Patent number: 11376441
    Abstract: Graph-theoretic segmentation methods for segmenting histological structures in H&E stained images of tissues. The method rely on characterizing local spatial statistics in the images. Also, a method for quantifying intratumor spatial heterogeneity that can work with single biomarker, multiplexed, or hyperplexed immunofluorescence (IF) data. The method is holistic in its approach, using both the expression and spatial information of an entire tumor tissue section and/or spot in a TMA to characterize spatial associations. The method generates a two-dimensional heterogeneity map to explicitly elucidate spatial associations of both major and minor sub-populations.
    Type: Grant
    Filed: May 19, 2020
    Date of Patent: July 5, 2022
    Assignee: University of Pittsburgh—Of the Commonwealth System of Higher Education
    Inventors: S. Chakra Chennubhotla, D. Lansing Taylor
  • Patent number: 11378517
    Abstract: A biological substance detection method for detecting a biological substance specifically in a pathological specimen, includes a step of immunologically staining the pathological specimen using a fluorescent label, a step of staining the pathological specimen with a staining reagent for morphology observation purposes (eosin) to observe the morphology of the pathological specimen, a step of irradiating the stained pathological specimen with excited light to cause the emission of a fluorescent and detecting the biological substance in the pathological specimen. In the step of immunologically staining the pathological specimen, a special fluorescent particle for which the excitation wavelength appears in a region that is different from the excitation wavelength region of eosin is used as the fluorescent label.
    Type: Grant
    Filed: April 11, 2018
    Date of Patent: July 5, 2022
    Assignee: KONICA MINOLTA, INC.
    Inventors: Kensaku Takanashi, Hideki Goda, Hisatake Okada, Yasushi Nakano, Kohsuke Gonda, Motohiro Takeda, Noriaki Ohuchi
  • Patent number: 11361561
    Abstract: Systems and methods discussed herein include, among other things, a method comprising quantifying analyte staining of a biological compartment in a region in which said staining is intermixed with analyte staining of an analytically-distinct distinct biological compartment. Disclosed systems and methods include, for example, a system and method for identifying membrane staining of an analyte of interest in regions where diffuse membrane staining is intermixed with cytoplasmic staining and/or punctate staining is disclosed. Disclosed systems and methods include, for example, a system and method for quantifying membrane staining of an analyte of interest in tissue or cytological samples having regions in which membrane staining is intermixed with cytoplasmic staining and/or punctate staining.
    Type: Grant
    Filed: July 8, 2020
    Date of Patent: June 14, 2022
    Assignee: Ventana Medical Systems, Inc.
    Inventors: Christophe Chefd'hotel, Kien Nguyen
  • Patent number: 11361438
    Abstract: In various embodiments, an experiment analysis application detects executional artifacts in experiments involving microwell plates. The experiment analysis application computes one or more sets of spatial features based on one or more heat maps associated with a microwell plate. The experiment analysis application then aggregates the set(s) of spatial features to generate a feature vector. The experiment analysis application inputs the feature vector into a trained classifier. In response, the trained classifier generates a label indicating that the microwell plate is associated with a first executional artifact.
    Type: Grant
    Filed: July 27, 2020
    Date of Patent: June 14, 2022
    Assignee: RECURSION PHARMACEUTICALS, INC.
    Inventors: Benjamin Marc Feder Fogelson, Peter McLean, Imran Haque, Marissa Saunders, Eric Fish, Charles Baker, Juan Sebastián Rodríguez Vera
  • Patent number: 11347044
    Abstract: A digital pathology imaging apparatus includes a single line scan camera sensor optically coupled with first and second optical paths. In a first embodiment, transmission mode illumination and oblique mode illumination are simultaneously used during a single stage movement that captures a low resolution macro image of the entire sample area and the entire label area of the slide via the first optical path. In a second embodiment, transmission mode illumination is used during a first stage movement that captures a low resolution macro image of at least the entire sample area via the first optical path and oblique mode illumination is used during a second stage movement that captures a low resolution macro image of at least the entire label area via the first optical path.
    Type: Grant
    Filed: October 16, 2020
    Date of Patent: May 31, 2022
    Assignee: Leica Biosystems Imaging, Inc.
    Inventors: Yunlu Zou, Nicholas Newberg, Gregory Crandall
  • Patent number: 11348661
    Abstract: A method for qualifying a specimen prepared on one or more hematoxylin and eosin (H&E) slides by assessing an expected yield of nucleic acids for tumor cells and providing associated unstained slides for subsequent nucleic acid analysis is provided.
    Type: Grant
    Filed: December 31, 2020
    Date of Patent: May 31, 2022
    Assignee: TEMPUS LABS, INC.
    Inventors: Stephen Yip, Irvin Ho, Lingdao Sha, Boleslaw Osinski, Aly Azeem Khan, Andrew J. Kruger, Michael Carlson, Abel Greenwald, Caleb Willis, Andrew Westley, Ryan Jones, Brett Mahon
  • Patent number: 11348238
    Abstract: A method for training a chromosome recognition model includes: identifying objects on a karyotype image, obtaining a mask and a minimal bounding box of each of the chromosome objects, and obtaining an organized image that includes a set of organized chromosome objects; generating a simulated metaphase image in which the chromosome objects are randomly reorganized; detecting the plurality of chromosome objects on the simulated metaphase image; obtaining a recalibrated image in which the chromosome objects are separated from one another, so as to train the chromosome recognition model for identifying feature of chromosome objects included in an image.
    Type: Grant
    Filed: June 22, 2020
    Date of Patent: May 31, 2022
    Assignee: Ever Fortune.AI CO., Ltd.
    Inventors: Christian Pascal Tchou, Fuu-Jen Tsai, Ken Ying-Kai Liao, Tzung-Chi Huang
  • Patent number: 11348239
    Abstract: A method for determining tumor block sufficiency for generating one or more hematoxylin and eosin (H&E) slides by assessing an expected yield of nucleic acids for tumor cells and determining a number of H&E slide for satisfying a desired total nucleic yield is provided.
    Type: Grant
    Filed: December 31, 2020
    Date of Patent: May 31, 2022
    Assignee: TEMPUS LABS, INC.
    Inventors: Stephen Yip, Irvin Ho, Lingdao Sha, Boleslaw Osinski, Aly Azeem Khan, Andrew J. Kruger, Michael Carlson, Abel Greenwald, Caleb Willis, Andrew Westley, Ryan Jones, Brett Mahon
  • Patent number: 11341648
    Abstract: An imaging system and method for microbial growth detection, counting or identification. One colony may be contrasted in an image that is not optimal for another type of colony. The system and method provides contrast from all available material through space (spatial differences), time (differences appearing over time for a given capture condition) and color space transformation using image input information over time to assess whether microbial growth has occurred for a given sample.
    Type: Grant
    Filed: May 15, 2020
    Date of Patent: May 24, 2022
    Assignee: Timothy M. Wiles
    Inventors: Timothy M. Wiles, Raphael Rodolphe Marcelpoil
  • Patent number: 11333661
    Abstract: A detection analyzer including a first sample input/output element, a second sample input/output element, a sample compartment, a vibration platform, a vibration generator, a data acquisition system, a laser converter, and a data display. The first sample input/output element and the second sample input/output element are each connected to the sample compartment; the vibration platform is located inside the sample compartment; the vibration generator is located outside the sample compartment, and the vibration platform is connected to the vibration generator; the data acquisition system is located outside the sample compartment, and is connected to the vibration platform; and the data display is connected to the data acquisition system.
    Type: Grant
    Filed: January 9, 2018
    Date of Patent: May 17, 2022
    Assignees: CIXI INSTITUTE OF BIOMEDICAL ENGINEERING NINGBO INSTITUTE OF INDUSTRIAL TECHNOLOGY, CHINESE ACADEMY OF SCIENCES, NINGBO INSTITUTE OF MATERIALS TECHNOLOGY & ENGINEERING, CHINESE ACADEMY OF SCIENCES
    Inventors: Fang Yang, Aiguo Wu
  • Patent number: 11320362
    Abstract: A lens-free microscope system for automatically analyzing yeast cell viability in a stained sample includes a portable, lens-free microscopy device that includes a housing containing a light source coupled to an optical fiber, the optical fiber spaced away several centimeters from an image sensor disposed at one end of the housing, wherein the stained sample is disposed on the image sensor or a sample holder adjacent to the image sensor. Hologram images are transferred to a computing device having image processing software contained therein, the image processing software identifying yeast cell candidates of interest from back-propagated images of the stained sample, whereby a plurality of spatial features are extracted from the yeast cell candidates of interest and subject to a trained machine learning model to classify the yeast cell candidates of interest as live or dead.
    Type: Grant
    Filed: September 22, 2017
    Date of Patent: May 3, 2022
    Assignee: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
    Inventors: Aydogan Ozcan, Alborz Feizi, Alon Greenbaum
  • Patent number: 11321836
    Abstract: An image-processing device including: a determination unit that determines a captured image on the basis of a ratio of cells and a distribution of the cells in the captured image acquired by imaging a sample including cells; and an area setting unit that sets an area used for analyzing the cells of the sample on the basis of a result of the determination acquired by the determination unit.
    Type: Grant
    Filed: April 2, 2020
    Date of Patent: May 3, 2022
    Assignees: NIKON CORPORATION, THE UNIVERSITY OF TOKYO
    Inventors: Mamiko Masutani, Kenta Imai, Wataru Tomosugi, Takuro Saigo, Chisako Iwamoto, Masafumi Yamashita, Kenichi Ohba, Masayuki Murata, Fumi Kano, Yoshiyuki Noguchi
  • Patent number: 11320642
    Abstract: This information processing apparatus includes: a search image acquisition unit that acquires enlarged images at focal positions different from each other; a first feature amount calculation unit that obtains, for each of the multiple captured enlarged images, a first feature amount based on a direct current component and a dynamic range of an alternating current component of pixel values for each of blocks forming each of the enlarged images; and an in-focus position determination unit that determines an in-focus position of each enlarged image based on the first feature amount.
    Type: Grant
    Filed: May 6, 2020
    Date of Patent: May 3, 2022
    Assignee: Sony Corporation
    Inventor: Takuya Oshima
  • Patent number: 11315250
    Abstract: Systems and methods for rapidly analyzing cell containing samples, for example to identify morphology or to localize and quantitate biomarkers are disclosed.
    Type: Grant
    Filed: February 26, 2019
    Date of Patent: April 26, 2022
    Assignee: YALE UNIVERSITY
    Inventors: David Rimm, Robert Camp
  • Patent number: 11314994
    Abstract: A mask structure optimization device includes a classification target image size acquisition unit that is configured to acquire a size of a classification target image which is an image including a classification target, a mask size setting unit that is configured to set a size of a mask applied to the classification target image, a brightness detection unit that is configured to detect a brightness of each pixel within the classification target image at a position on an opposite side of the mask from the classification target image, a sum total brightness calculation unit that is configured to calculate the sum total brightness of the each pixel within the classification target image detected by the brightness detection unit, an initial value setting unit that is configured to set an initial value for a mask pattern of the mask, and a movement unit that is configured to relatively move the mask with respect to the classification target image.
    Type: Grant
    Filed: January 30, 2019
    Date of Patent: April 26, 2022
    Inventors: Issei Sato, Masahiro Kazama, Masashi Ugawa, Hiroaki Adachi, Fumiya Shimada
  • Patent number: 11315292
    Abstract: Systems and methods of using the same for functional fluorescence imaging of live cells in suspension with isotropic three dimensional (3D) diffraction-limited spatial resolution are disclosed. The method-live cell computed tomography (LCCT)-in-volves the acquisition of a series of two dimensional (2D) pseudo-projection images from different perspectives of the cell that rotates around an axis that is perpendicular to the optical axis of the imaging system. The volumetric image of the cell is then tomographically reconstructed.
    Type: Grant
    Filed: March 2, 2018
    Date of Patent: April 26, 2022
    Assignee: ARIZONA BOARD OF REGENTS ON BEHALF OF ARIZONA STATE UNIVERSITY
    Inventors: Deirdre Meldrum, Roger Johnson, Laimonas Kelbauskas, Jeff Houkal, Brian Ashcroft, Dean Smith, Hong Wang, Shih-Hui (Joseph) Chao, Rishabh Shetty, Jakrey Myers, Iniyan Soundappa Elango
  • Patent number: 11298010
    Abstract: Disclosed herein are devices having both imaging components and cell collection apparatuses. More specifically, the various embodiments disclosed herein include swallowable capsules having deployable collection structures, expansion structures to deploy the collection structures, and imaging components.
    Type: Grant
    Filed: January 31, 2018
    Date of Patent: April 12, 2022
    Assignees: University of Kansas, Department of Veterans Affairs
    Inventors: Ajay Bansal, Lane Christenson, Nick Baker, Ryan Baskins, John Stark, Nourouddin Sharifi, Hadi Kh A Kh Alzuabi, Parker Gill
  • Patent number: 11287629
    Abstract: A method for determining a height position of an object at of using a microscope which images using a point-spread function along a z-direction (height direction), comprising the steps of imaging the object in the far field and determining a far-field intensity, calculating a maximum intensity expected by multiplying the far-field intensity by a scaling factor, partially confocally imaging the object with the focus in the z-direction within the depth-of-field range, and determining a partially-confocal intensity of the imaging, calculating the intensity of the point-spread function (at the first location) by forming a difference between the partially-confocal intensity and a product of the far-field intensity and a predefined combination factor, calculating the z-coordinate of the focus at a point-spread function maximum, using a previously-known form of the point spread function, its calculated intensity, and the calculated expected maximum intensity, and using the z-coordinate as the height position of the
    Type: Grant
    Filed: April 21, 2021
    Date of Patent: March 29, 2022
    Assignee: Carl Zeiss Microscopy GmbH
    Inventors: Nils Langholz, Viktor Drescher
  • Patent number: 11282201
    Abstract: Provided is an information processing device that includes a first information acquisition section that acquires first information on the basis of a still image in a frame corresponding to a predetermined time point, from among a plurality of images of a biological sample captured in a time-series manner, a second information acquisition section that acquires second information on the basis of an interframe change of the plurality of images in a predetermined period, and a determination section that determines an event regarding the biological sample, using the first information and the second information.
    Type: Grant
    Filed: October 18, 2017
    Date of Patent: March 22, 2022
    Assignee: SONY CORPORATION
    Inventors: Rei Murata, Shinji Watanabe
  • Patent number: 11280991
    Abstract: In an image acquisition device, when capturing an optical image of a sample S through lane scanning, the number of tile images T included in a tile image row R acquired in one lane is counted, and a determination is made as to whether or not the number of images reaches a planned acquisition count that is set in advance. In a case where it is determined that the number of images does not reach the planned acquisition count, lane scanning with respect to the lane is re-executed. Accordingly, even when a loss of the tile images T occurs due to an environment load, the tile images T are complemented by re-execution of the lane scanning, and thus it is possible to prevent the loss of the tile images T in an observation image.
    Type: Grant
    Filed: May 28, 2018
    Date of Patent: March 22, 2022
    Assignee: HAMAMATSU PHOTONICS K.K.
    Inventors: Akira Takahashi, Takayuki Kawashima, Jinichi Susuki
  • Patent number: 11276172
    Abstract: Various image diagnostic systems, and methods of operating thereof, are disclosed herein. Example embodiments relate to operating the image diagnostic system to identify one or more tissue types within an image patch according to a hierarchical histological taxonomy, identifying an image patch associated with normal tissue, generating a pixel-level segmented image patch for an image patch, generating an encoded image patch for an image patch of at least one tissue, searching for one or more histopathological images, and assigning an image patch to one or more pathological cases.
    Type: Grant
    Filed: May 1, 2020
    Date of Patent: March 15, 2022
    Assignee: Huron Technologies International Inc.
    Inventors: Mahdi S. Hosseini, Konstantinos N. Plataniotis, Lyndon Chan, Jasper Hayes, Savvas Damaskinos
  • Patent number: 11268883
    Abstract: Provided are: a past relation identification unit that combines past sensor data with job data based on information on an item relating to data combination, and identifies a data relation between items based on an item of the past sensor data and an item of the job data; a real-time relation identification unit that identifies a data relation between items of real-time sensor data; a similarity calculation unit that calculates a similarity between the data relation identified by the past relation identification unit and the data relation identified by the real-time relation identification unit; and an output controller that outputs the data relation identified by the past relation identification unit and the data relation identified by the real-time relation identification unit while associating with each other by the item of the past sensor data and the item of the real-time sensor data, the similarity between those data relations exceeding a threshold, the similarity being calculated by the similarity calcu
    Type: Grant
    Filed: March 13, 2019
    Date of Patent: March 8, 2022
    Assignee: HITACHI, LTD.
    Inventors: Kenji Kawasaki, Hidenori Yamamoto, Takeshi Handa, Takashi Tsuno
  • Patent number: 11272142
    Abstract: In a system and method for determining vital sign information of a subject the subject is illuminated with radiation, and radiation reflected from the subject is received. A region of interest is located in a first phase. Said illumination is controlled to locally illuminate, in a second phase, the located region of interest with radiation allowing determination of vital sign information. Finally, vital sign information of the subject is determined from the radiation reflected from said region of interest and detected in said second phase.
    Type: Grant
    Filed: February 28, 2014
    Date of Patent: March 8, 2022
    Assignee: Koninklijke Philips N.V.
    Inventors: Erik Bresch, Jens Muehlsteff, Rolf Neumann, Mukul Julius Rocque, Willem Verkruijsse
  • Patent number: 11268880
    Abstract: A method for detecting lens cleanliness of a lens in a flat-field optical path, the flat-field optical path includes a light source, the lens, a camera, the light source is a narrow-band multispectral uniform surface light source, the camera's light-sensitive surface is disposed perpendicular to an optical axis of the lens and in the light position of the lens and a pupil interposed between the lens and the light source, the method including collecting the bright-field image data and dark-field image data for a plurality of pupil aperture sizes through the lens; for each pixel, performing a pupil differential flat field correction to yield a plurality of PiPj pupil differentials; and displaying the pupil differentials in the form of a plurality of images to show uniformity of each image, wherein a non-uniform area on each image is determined to have been caused by an impurity of the lens.
    Type: Grant
    Filed: September 24, 2021
    Date of Patent: March 8, 2022
    Assignee: MLOptic Corp.
    Inventors: Jiang He, Teresa Zhang, Wei Zhou, Weida Liu
  • Patent number: 11262308
    Abstract: Methods and devices for cytometric analysis are provided. A cytometry apparatus is provided which may be used with a stationary sample cuvette for analysis of a stationary sample or with a flow sample cuvette for analysis of a flowing sample. The methods and devices provided herein may be used to perform cytometric analysis of samples under a wide range of experimental and environmental conditions.
    Type: Grant
    Filed: October 23, 2020
    Date of Patent: March 1, 2022
    Assignee: Labrador Diagnostics LLC
    Inventors: Chinmay Pangarkar, Karan Mohan
  • Patent number: 11263551
    Abstract: A method for machine-learning based process flow recommendation is provided. The method may include training a machine-learning model by at least processing training data with the machine-learning model. The training data may include a matrix representing one or more existing process flows by at least indicating actions that are performed on a document object to generate a subsequent document object. An indication that a first document object is created as part of a process flow may be received. In response to the indication, the trained machine-learning model may be applied to generate a recommendation to perform, as part of the process flow, an action to generate a second document object. Related systems and articles of manufacture, including computer program products, are also provided.
    Type: Grant
    Filed: November 8, 2018
    Date of Patent: March 1, 2022
    Assignee: SAP SE
    Inventors: Kavitha Krishnan, Kumar Nitesh, Naga Sai Narasimha Guru Charan Koduri
  • Patent number: 11250568
    Abstract: Techniques for processing multiplexed immunofluorescence (MxIF) images. The techniques include obtaining at least one MxIF image of a same tissue sample, obtaining information indicative of locations of cells in the at least one MxIF image, identifying multiple groups of cells in the at least one MxIF image at least in part by determining feature values for at least some of the cells using the at least one MxIF image and the information indicative of locations of the at least some cells in the at least one MxIF image and grouping the at least some of the cells into the multiple groups using the determined feature values, and determining at least one characteristic of the tissue sample using the multiple cell groups.
    Type: Grant
    Filed: March 6, 2021
    Date of Patent: February 15, 2022
    Assignee: BostonGene Corporation
    Inventors: Viktor Svekolkin, Ilia Galkin, Ekaterina Postovalova, Ravshan Ataullakhanov, Alexander Bagaev, Arina Varlamova, Pavel Ovcharov
  • Patent number: 11243387
    Abstract: Systems and methods for capturing a digital image of a slide using an imaging line sensor and a focusing line sensor. In an embodiment, a beam-splitter is optically coupled to an objective lens and configured to receive one or more images of a portion of a sample through the objective lens. The beam-splitter simultaneously provides a first portion of the one or more images to the focusing sensor and a second portion of the one or more images to the imaging sensor. A processor controls the stage and/or objective lens such that each portion of the one or more images is received by the focusing sensor prior to it being received by the imaging sensor. In this manner, a focus of the objective lens can be controlled using data received from the focusing sensor prior to capturing an image of a portion of the sample using the imaging sensor.
    Type: Grant
    Filed: November 25, 2020
    Date of Patent: February 8, 2022
    Assignee: Leica Biosystems Imaging, Inc.
    Inventors: Yunlu Zou, Gregory Crandall, Allen Olson
  • Patent number: 11244445
    Abstract: According to one embodiment, a medical information processing apparatus includes processing circuitry. The processing circuitry is configured to acquire information on an accuracy of a trained model that outputs information on a lesion included in data related to a medical image based on inputted data related to the medical image. Further, the processing circuitry is configured to determine a data collection condition for the medical image in a medical image diagnostic apparatus based on the information on the accuracy.
    Type: Grant
    Filed: September 19, 2019
    Date of Patent: February 8, 2022
    Assignee: CANON MEDICAL SYSTEMS CORPORATION
    Inventor: Kenta Moriyasu
  • Patent number: 11243389
    Abstract: A method is used for optical scanning of at least one object region placed on a transparent specimen holder. The method is as follows: for each sample lateral position of plural predefined sample lateral positions performing a focus determination by: performing laser reflection and using a first camera taking plural first images to determine a reference distance between the specimen holder and an objective lens; performing transmission flash illumination and using a second camera taking plural second images to define a focus distance taking into account the reference distance; after completing the focus determination, determining a focus distance topology across the object region based on the focus distances determined for ail sample lateral positions; and laterally moving the specimen holder and acquiring third images while focusing according to the focus distance topology.
    Type: Grant
    Filed: April 9, 2018
    Date of Patent: February 8, 2022
    Assignee: EUROIMMUN Medizinische Labordiagnostika AG
    Inventors: Christian Feirer, Erik Bernitt, Marc Paul, Rafael Roznowicz
  • Patent number: 11238581
    Abstract: Disclosed are a method for computer-assisted evaluation of biomarkers in a tissue sample as well as a corresponding system and computer program. The method includes the following steps: providing an image of the tissue sample in which cell nuclei of the tissue as well as biomarkers are represented, automatically determining for a plurality of locations or regions within the image a blood vessel probability that represents a probability that the image at the corresponding location or region represents a blood vessel, automatically determining an assumed blood vessel location within the image based on a distribution of blood vessel probability with respect to the locations or regions within the image, automatically selecting biomarkers represented in the image at locations outside the assumed blood vessel location but within a threshold distance from the assumed blood vessel location, and automatically evaluating the selected biomarkers.
    Type: Grant
    Filed: September 19, 2018
    Date of Patent: February 1, 2022
    Assignees: Deutsches Krebsforschungszentrum Stiftung des öffentlichen Rechts, Brandenburgische Technische Universität Cottbus-Senftenberg, GA Generic Assays GmbH
    Inventors: Cläre Von Neubeck, Rico Hiemann, Alexey Elimport
  • Patent number: 11238268
    Abstract: An image processing device and an image processing method capable of clarifying a specific region, especially a stained region of the cell nucleus, by correcting in consideration of overlap of staining, thereby making discrimination easy, and a pathological diagnosis assistance system using the same are provided. A pathological stained image is decomposed into dye concentration images of respective dyes, dye information on the respective staining dyes are correlated, and the respective dye concentration images are corrected to emphasize a specific region.
    Type: Grant
    Filed: May 24, 2018
    Date of Patent: February 1, 2022
    Assignee: KONICA MINOLTA, INC.
    Inventor: Yuki Kazayama
  • Patent number: 11232567
    Abstract: As the capabilities of digital histopathology machines grows, there is an increasing need to ease the burden on pathology professionals of finding interesting structures in such images. Digital histopathology images can be at least several Gigabytes in size, and they may contain millions of cell structures of interest. Automated algorithms for finding structures in such images have been proposed, such as the Active Contour Model (ACM). The ACM algorithm can have difficulty detecting regions in images having variable colour or texture distributions. Such regions are often found in images containing cell nuclei, because nuclei do not always have a homogeneous appearance. The present application describes a technique to identify inhomogeneous structures, for example, cell nuclei, in digital histopathology information. It is proposed to search pre-computed super-pixel information using a morphological variable, such as a shape-compactness metric, to identify candidate objects.
    Type: Grant
    Filed: May 24, 2017
    Date of Patent: January 25, 2022
    Assignee: KONINKLIJKE PHILIPS N.V.
    Inventor: Christiaan Varekamp