Cell Analysis, Classification, Or Counting Patents (Class 382/133)
  • Patent number: 10254530
    Abstract: An imaging method including: controlling luminance distribution of light such that the light continuously attenuates from an inner side toward an edge in a peripheral area within an imaging range of an imaging unit; emitting, in the imaging range, the light whose luminance distribution has been controlled; imaging an object within the imaging range where the light has been emitted to generate image data; sequentially moving the imaging range relative to the object such that peripheral areas where the light attenuates overlap with each other in adjacent imaging ranges; sequentially obtaining a plurality of images with different imaging ranges based on the image data; and creating a combined image by stitching the plurality of images in such a way that adjacent images of the plurality of images corresponding to the adjacent imaging ranges are stitched together such that image areas corresponding to the peripheral areas overlap with each other.
    Type: Grant
    Filed: March 9, 2016
    Date of Patent: April 9, 2019
    Assignee: OLYMPUS CORPORATION
    Inventor: Takashi Miyoshi
  • Patent number: 10241310
    Abstract: A method of forming an imaging calibration device for a biological material imaging system, the method comprises: providing one or more discrete regions upon or within a retaining member, each region for the receipt of a selected predetermined biological stain material; selecting one or more predetermined biological stain materials, wherein each of the selected predetermined biological stain materials has a predetermined optical response, and providing one or more of the selected predetermined biological stain materials to the said one or more discrete regions such that the said material is localised in the said region.
    Type: Grant
    Filed: June 3, 2013
    Date of Patent: March 26, 2019
    Assignee: FFEI Limited
    Inventors: Martin Philip Gouch, Craig Revie, Peter Walsh, Jacqueline Margaret Deane
  • Patent number: 10236166
    Abstract: A lenslet based integral field spectrograph (IFS) may have a design that makes better use of the detector pixels by placing adjacent spectra next to each other rather than staggering the spectra. Such a design maintains the main compactness and simplicity of prior lenslet array based IFSs, but improves the detector efficiency, which is rather low in conventional lenslet array based IFSs.
    Type: Grant
    Filed: June 9, 2017
    Date of Patent: March 19, 2019
    Assignee: The United States of America as represented by the Administrator of the National Aeronautics and Space Administration
    Inventors: Qian Gong, David A. Content, Michael W. McElwain, Avram Max Mandell, Tyler D. Groff, Maxime J. Rizzo
  • Patent number: 10235750
    Abstract: A method is proposed for identifying an anatomical structure within a spatial-temporal image (i.e. a series of frames captured as respective times). A current frame of spatial-temporal medical image is processed using information from one or more previous and/or subsequent temporal frames, to aid in the segmentation of an object or a region of interest (ROI) in a current frame. The invention is applicable to both two- and three-dimensional spatial-temporal images (i.e., 2D+time or 3D+time), and in particular to cardiac magnetic resonance (CMR images). An initialization process for this method segments the left ventricle (LV) in a CMR image by a fuzzy c-means (FCM) clustering algorithm which employs a circular shape function as part of the definition of the dissimilarity measure.
    Type: Grant
    Filed: February 27, 2015
    Date of Patent: March 19, 2019
    Assignees: Agency for Science, Technology and Research, Singapore Health Services Pte Ltd
    Inventors: Xulei Yang, Yi Su, Si Yong Yeo, Liang Zhong, Ru San Tan
  • Patent number: 10235559
    Abstract: The present disclosures relates to a method of detecting, classifying, and counting dots in an image of a tissue specimen comprising detecting dots in an image of the tissue sample that meet criteria for absorbance strength, black unmixed image channel strength, red unmixed image channel strength, and a difference of Gaussian threshold, wherein the detected dots correspond to in situ hybridization signals in the tissue samples; classifying the detected dots as belonging to a black in situ hybridization signal or to a red in situ hybridization signal; and calculating a ratio of those dots belonging to the black in situ hybridization signal and those belonging to the red in situ hybridization signal.
    Type: Grant
    Filed: July 25, 2017
    Date of Patent: March 19, 2019
    Assignee: Ventana Medical Systems, Inc.
    Inventors: Anindya Sarkar, Jim Martin
  • Patent number: 10234442
    Abstract: The present invention discloses a method for finding a cell nucleus of a target cell from a cell image, wherein the cell image includes the target cell and at least one variation cell, and the target cell includes cytoplasm and the cell nucleus. The method includes steps of: (a) processing the cell image via an image processor such that the cytoplasm, the cell nucleus and the variation cell have different shades of color; (b) demarcating the outlines of the cytoplasm, the cell nucleus and the variation cell; (c) calculating geometrical reference points of the outlines; (d) calculating the distances from the geometrical reference point of the cytoplasm outline to the geometrical reference point of the cell nucleus outline and to the geometrical reference points of the variation cell outlines; and (e) finding a specific geometrical reference point having a shortest distance to locate a specific outline corresponding to the specific geometrical reference point as the cell nucleus.
    Type: Grant
    Filed: November 29, 2016
    Date of Patent: March 19, 2019
    Assignee: METAL INDUSTRIES RESEARCH & DEVELOPMENT CENTRE
    Inventors: Ming-Hui Cheng, Yan-Jun Chen, Tsung-Chih Yu, Bo-Wei Pan, Chun-Sen Wu
  • Patent number: 10208352
    Abstract: There is provided a canceration information providing method which can detect the possibility of cancer in the initial stage with high accuracy. The canceration information providing method for providing information pertaining to canceration of cells includes: acquiring measurement data including first data pertaining to size of a cell nucleus and second data pertaining to size of a cytoplasm for each cell contained in a measurement sample which includes cells collected from epithelial tissue; extracting the measurement data of cells to be analyzed, which are at least some of the cells located toward the basal membrane side of the cells existing in the surface layer in the epithelial tissue, from the measurement data of a plurality of cells in the measurement sample based on the first data and the second data acquired for each cell; and analyzing the extracted measurement data and outputting the information pertaining to the canceration of cells.
    Type: Grant
    Filed: January 21, 2014
    Date of Patent: February 19, 2019
    Assignee: SYSMEX CORPORATION
    Inventors: Ryuichiro Ebi, Koki Tajima, Shigeki Abe, Masanori Kawano
  • Patent number: 10198832
    Abstract: Methods, systems, and apparatus, including computer programs encoded on a computer storage medium, for generating a final classification output for an image of eye tissue. The image is provided as input to each of one or more segmentation neural networks to obtain one or more segmentation maps of the eye tissue in the image. A respective classification input is generated from each of the segmentation maps. For each of the segmentation maps, the classification input for the segmentation map is provided as input to each of one or more classification neural networks to obtain, for each segmentation map, a respective classification output from each classification neural network. A final classification output for the image is generated from the respective classification outputs for each of the segmentation maps.
    Type: Grant
    Filed: June 28, 2018
    Date of Patent: February 5, 2019
    Inventors: Jeffrey De Fauw, Joseph R. Ledsam, Bernardino Romera-Paredes, Stanislav Nikolov, Nenad Tomasev, Samuel Blackwell, Harry Askham, Xavier Glorot, Balaji Lakshminarayanan, Trevor Back, Mustafa Suleyman, Pearse A. Keane, Olaf Ronneberger, Julien Robert Michel Cornebise
  • Patent number: 10192306
    Abstract: A nucleolus detection unit, which detects nucleoli in a plurality of cells in a cell image obtained by imaging the cells, and a cell recognition unit, which acquires information indicating a distance between the nucleoli and recognizes the individual cells based on the information indicating the distance, are provided.
    Type: Grant
    Filed: September 23, 2016
    Date of Patent: January 29, 2019
    Assignee: FUJIFILM Corporation
    Inventor: Kenta Matsubara
  • Patent number: 10191264
    Abstract: Systems and methods for capturing a digital image of a slide using an imaging line sensor and a focusing line sensor. In an embodiment, a beam-splitter is optically coupled to an objective lens and configured to receive one or more images of a portion of a sample through the objective lens. The beam-splitter simultaneously provides a first portion of the one or more images to the focusing sensor and a second portion of the one or more images to the imaging sensor. A processor controls the stage and/or objective lens such that each portion of the one or more images is received by the focusing sensor prior to it being received by the imaging sensor. In this manner, a focus of the objective lens can be controlled using data received from the focusing sensor prior to capturing an image of a portion of the sample using the imaging sensor.
    Type: Grant
    Filed: December 11, 2017
    Date of Patent: January 29, 2019
    Assignee: LEICA BIOSYSTEMS IMAGING, INC.
    Inventors: Yunlu Zou, Gregory Crandall, Allen Olson
  • Patent number: 10181015
    Abstract: A processor receives a first list comprising a plurality of events from a portion of digital data of an unknown work and one or more metrics between each pair of adjacent events from the plurality of events. The processor compares the first list to a second list comprising events and metrics between events for a known work to determine a first quantity of hits and a second quantity of misses. The processor determines whether the first list matches the second list based on the first quantity of hits and the second quantity of misses. The processor determines that the unknown work is a copy of the known work responsive to determining that the first list matches the second list.
    Type: Grant
    Filed: October 9, 2017
    Date of Patent: January 15, 2019
    Assignee: Audible Magic Corporation
    Inventor: Erling Wold
  • Patent number: 10180387
    Abstract: The identification apparatus includes a quantitative phase image acquisition unit, a feature quantity extraction unit, a learning unit, a storage unit, and an identification unit. The feature quantity extraction unit extracts a feature quantity of a quantitative phase image of a cell. The learning unit performs machine learning for a quantitative phase image of a known cell of which a type is known based on the feature quantity extracted by the extraction unit. The storage unit stores a result of the machine learning by the learning unit. The identification unit determines, based on the feature quantity extracted by the extraction unit for the quantitative phase image of an unknown cell of which a type is unknown, the type of the unknown cell using the learning result stored by the storage unit.
    Type: Grant
    Filed: July 23, 2015
    Date of Patent: January 15, 2019
    Assignees: NATIONAL UNIVERSITY CORPORATION HAMAMATSU UNIVERSITY SCHOOL OF MEDICINE, HAMAMATSU PHOTONICS K.K.
    Inventors: Yusuke Ozaki, Hidenao Iwai, Hiroyuki Konno, Hirotoshi Kikuchi, Toyohiko Yamauchi
  • Patent number: 10176577
    Abstract: Certain aspects of an apparatus and method for automatic ER/PR scoring of tissue samples may include for determining a cancer diagnosis score comprising identifying a positive stained nucleus in a slide image of the tissue sample, identifying a negative stained nucleus in the slide image, computing a proportion score based on number of the positive stained nucleus identified and number of the negative stained nucleus identified and determining the cancer diagnosis score based on the proportion.
    Type: Grant
    Filed: April 24, 2018
    Date of Patent: January 8, 2019
    Assignee: SONY CORPORATION
    Inventors: Ming-Chang Liu, Ximin Zhang
  • Patent number: 10168520
    Abstract: Provided is a microscope system including a motor-driven stage on which is mounted a culture vessel containing one or more cell clusters, each including cells having a target molecule labeled with a fluorescent or luminescent chemical; a low-magnification-image acquiring unit that acquires low-magnification images of the cell clusters in the culture vessel mounted on the stage; a detecting unit that detects the position of each cell cluster in the culture vessel by analyzing the acquired low-magnification images; and a high-magnification-image acquiring unit that, after the detected position is aligned with the optical axis of an objective lens, acquires slice images of fluorescence or luminescence emitted from the cells forming the cell cluster at a higher magnification than the low-magnification-image acquiring unit at intervals along the optical axis while the stage and/or the lens is moved to change stepwise the distance between the lens and the cell cluster.
    Type: Grant
    Filed: May 23, 2016
    Date of Patent: January 1, 2019
    Assignee: OLYMPUS CORPORATION
    Inventors: Hisao Kitagawa, Yoshihiro Shimada, Kosuke Takagi
  • Patent number: 10168526
    Abstract: An cell contour formation apparatus includes a cell image acquiring unit, a subband image creating unit, a features calculating unit, a correcting unit, a contour forming unit. The subband image creating unit creates subband images including a low frequency image and a high frequency image. The features calculating unit calculates a local texture features from the high frequency image. The correcting unit corrects the high frequency image on the basis of the pixel value of the low frequency image and the texture features. The contour forming unit forms contours of cells included in the cell group on the basis of the corrected high frequency image.
    Type: Grant
    Filed: June 27, 2014
    Date of Patent: January 1, 2019
    Assignee: OLYMPUS CORPORATION
    Inventor: Hideya Aragaki
  • Patent number: 10163218
    Abstract: An image analysis device includes a first calculating part that calculates a magnitude of motion of an image element from time-series images of pulsating myocardial cells, a second calculating part that calculates a feature quantity on the basis of the magnitude of the motion of the image element calculated by the first calculating part, and an identifying part that identifies whether the image element is a predetermined image element on the basis of the feature quantity calculated by the second calculating part.
    Type: Grant
    Filed: September 12, 2014
    Date of Patent: December 25, 2018
    Assignee: NIKON CORPORATION
    Inventors: Kei Nakagawa, Masafumi Mimura, Takayuki Uozumi
  • Patent number: 10163203
    Abstract: [Object] To provide a cell analysis system, a cell analysis program and a cell analysis method suitable for analyzing the movement of ions or molecules across cell membranes. [Solving Means] A cell analysis system according to the present disclosure includes a motion information extracting unit and a motion characteristics calculating unit. The motion information extracting unit extracts motion information arising from a movement of ions or molecules across a cell membrane, out of a cell image obtained from imaging a cell in time series. The motion characteristics calculating unit calculates motion characteristics of the motion information.
    Type: Grant
    Filed: September 18, 2014
    Date of Patent: December 25, 2018
    Assignee: Sony Corporation
    Inventors: Kazuhiro Nakagawa, Takuya Kishimoto, Eriko Matsui
  • Patent number: 10151746
    Abstract: A sample analyzer prepares a measurement sample from a blood sample or a body fluid sample which differs from the blood sample; measures the prepared measurement sample; obtains characteristic information representing characteristics of the components in the measurement sample; sets either a blood measurement mode for measuring the blood sample, or a body fluid measurement mode for measuring the body fluid sample as an operating mode; and measures the measurement sample prepared from the blood sample by executing operations in the blood measurement mode when the blood measurement mode has been set, and measuring the measurement sample prepared from the body fluid sample by executing operations in the body fluid measurement mode that differs from the operations in the blood measurement mode when the body fluid measurement mode has been set, is disclosed. A computer program product is also disclosed.
    Type: Grant
    Filed: February 28, 2018
    Date of Patent: December 11, 2018
    Assignee: SYSMEX CORPORATION
    Inventors: Takaaki Nagai, Noriyuki Narisada, Daigo Fukuma, Masanori Imazu
  • Patent number: 10147182
    Abstract: A method for calibrating a biological instrument is provided. The method comprises the steps of acquiring an image of at least one biological sample array, determining a first region of interest within the image, wherein the first region of interest comprises a first plurality of locations on the at least one biological array; and identifying within the first region of interest, a plurality of image elements associated with each of the first plurality of locations on the at least one biological array.
    Type: Grant
    Filed: November 14, 2016
    Date of Patent: December 4, 2018
    Assignee: LIFE TECHNOLOGIES CORPORATION
    Inventors: Jacob Freudenthal, Jeffrey Marks, Francis Cheng, Thomas Wessel
  • Patent number: 10140419
    Abstract: A graphical user interface on a computer provides for the analysis of location specific data and the presentation of analysis results for visual comparison by a user. Results of the analysis are visually presented as icons subdivided into regions and arranged in such a way that the user is able to associate each icon with a data location. A visual presentation of results in the icons and regions allows a user to visually compare the analysis results in two or more data sets according to location. The graphical user interface further provides for the definition and adjustment of an analysis through the interaction of a user with a graphical representation of the analysis. In some cases, the visual presentation of results tracks the analysis adjustments so the user can visually observe the effects that the adjustments have on the results. A method of interacting with the interface to define an analysis and represent results and a method of presenting two or more data sets using the interface are described.
    Type: Grant
    Filed: January 16, 2015
    Date of Patent: November 27, 2018
    Assignee: EMD Millipore Corporation
    Inventors: Amedeo Joseph Cappione, III, Ray Lefebvre, Neil Kilcoin, David Andrew King, George J. Dixon
  • Patent number: 10127696
    Abstract: One or more embodiments may include techniques to computer generate one or more plots based on computational clustering performed by a system. Embodiments include performing clustering on a dataset to generate a number of clusters of data for the dataset. The clusters may be processed and used to generate the one or more plots. In some embodiments, the plots may include one or more variables plotted against a weighted average score associated with a cluster, the plot may visually indicate the effect that the one or more variables has on the predicted outcome. The one or more plots may be presented in a display on a display device. In some embodiments, the plots may be segmented and each segment may correspond with a number of individual curves. The segmented curves may be plotted and displayed on the display device.
    Type: Grant
    Filed: March 21, 2018
    Date of Patent: November 13, 2018
    Assignee: SAS Institute Inc.
    Inventors: Raymond Eugene Wright, Ilknur Kaynar Kabul, Susan Edwards Haller
  • Patent number: 10121245
    Abstract: Systems and methods are provided for identifying markers for inflammation in a tissue image. The tissue image is captured as an image of a histology slide. Subcellular structures in the tissue image are segmented via a first automated process to identify at least one variety of immune cells within the image. Glands and vilii are identified within the tissue image via a second automated process. Neutrophils are identified within the tissue image via a third automated process. An output representing the identified glands, villi, neutrophils, and other immune cells is provided to a human operator.
    Type: Grant
    Filed: September 14, 2016
    Date of Patent: November 6, 2018
    Assignee: UNIVERSITY OF NOTRE DAME
    Inventors: Danny Ziyi Chen, Jiazhuo Wang, John DeWolfe MacKenzie, Rageshree Ramachandran
  • Patent number: 10115013
    Abstract: Accurate localization of isolated particles is important in single particle based super-resolution microscopy. It allows the imaging of biological samples with nanometer-scale resolution using a simple fluorescence microscopy setup. Nevertheless, conventional techniques for localizing single particles can take minutes to hours of computation time because they require up to a million localizations to form an image. In contrast, the present particle localization techniques use wavelet-based image decomposition and image segmentation to achieve nanometer-scale resolution in two dimensions within seconds to minutes. This two-dimensional localization can be augmented with localization in a third dimension based on a fit to the imaging system's point-spread function (PSF), which may be asymmetric along the optical axis. For an astigmatic imaging system, the PSF is an ellipse whose eccentricity and orientation varies along the optical axis.
    Type: Grant
    Filed: July 17, 2015
    Date of Patent: October 30, 2018
    Assignee: CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE (CNRS)
    Inventor: Jean-Baptiste Sibarita
  • Patent number: 10114003
    Abstract: A stem cell differentiation determination device includes an observation image acquisition unit that captures an image of an observation region including a stem cell in time series to acquire at least two observation images, a feature amount acquisition unit that acquires at least one feature amount of the stem cell for each observation image, a determination unit that determines whether or not the stem cell has been differentiated, on the basis of the feature amount, a change information acquisition unit that acquires information about a change in the feature amount between the observation images captured in time series or information about a change in a determination result from undifferentiation to differentiation between the observation images, and an output unit that outputs the information about a change in the feature amount or the information about a change in the determination result.
    Type: Grant
    Filed: February 17, 2016
    Date of Patent: October 30, 2018
    Assignee: FUJIFILM Corporation
    Inventor: Takayuki Tsujimoto
  • Patent number: 10108779
    Abstract: Automatic nuclear segmentation. In an embodiment, a plurality of superpixels are determined in a digital image. For each of the superpixels, any superpixels located within a search radius from the superpixel are identified, and, for each unique local combination between the superpixel and any identified superpixels located within the search radius from the superpixel, a local score for the local combination is determined. One of a plurality of global sets of local combinations with an optimum global score is identified based on the determined local scores.
    Type: Grant
    Filed: December 12, 2016
    Date of Patent: October 23, 2018
    Assignee: LEICA BIOSYSTEMS IMAGING, INC.
    Inventors: Walter Georgescu, Kiran Saligrama, Allen Olson, Bharat Annaldas
  • Patent number: 10102665
    Abstract: Described embodiments include a system that includes a display and a processor. The processor is configured to position an indicator, in response to a positioning input from a user, over a particular point on a three-dimensional electroanatomical map that is displayed on the display, and over which are displayed a plurality of markers that mark respective data points. The processor is further configured to expand a contour, subsequently, along a surface of the map, while a selecting input from the user is ongoing, such that all points on the contour remain equidistant, at an increasing geodesic distance with respect to the surface, from the particular point, and to display, on the display, one or more properties of each of the data points that is marked by a respective one of the markers that is inside the contour. Other embodiments are also described.
    Type: Grant
    Filed: December 30, 2016
    Date of Patent: October 16, 2018
    Assignee: Biosense Webster (Israel) Ltd.
    Inventors: Assaf Cohen, Fady Massarwi, Ido Ilan
  • Patent number: 10089556
    Abstract: An artificial neural network for analyzing input data, the input data being a 3D tensor having D channels, such as D frames of a video snippet, to recognize an action therein, including: D spatial transformer modules, each generating first and second spatial transformations and corresponding first and second attention windows using only one of the D channels, and transforming first and second regions of each of the D channels corresponding to the first and second attention windows to generate first and second patch sequences; first and second CNNs, respectively processing a concatenation of the D first patch sequences and a concatenation of the D second patch sequences; and a classification network receiving a concatenation of the outputs of the first and second CNNs and the D sets of transformation parameters of the first transformation outputted by the D spatial transformer modules, to generate a predicted action class.
    Type: Grant
    Filed: June 12, 2017
    Date of Patent: October 2, 2018
    Assignee: KONICA MINOLTA LABORATORY U.S.A., INC.
    Inventor: Ting Xu
  • Patent number: 10061108
    Abstract: The present invention relates to the field of digital pathology and in particular to whole slide scanners. Autofocus imaging can be performed by sampling a first number of pixels of a primary image sensor and sampling a second number of pixels of an autofocus image sensor, wherein the second number is between one quarter and three quarters of the first number. Thus, continuous autofocus for rapid light scanning may be provided based on an additional image sensor that is tilted with respect to the optical axis.
    Type: Grant
    Filed: May 4, 2011
    Date of Patent: August 28, 2018
    Assignee: Koninklijke Philips N.V.
    Inventors: Bas Hulsken, Sjoerd Stallinga
  • Patent number: 10052038
    Abstract: A device for determining a vital sign of a subject comprises an interface that receives a data stream derived from detected electromagnetic radiation reflected from a region of interest including a skin area of the subject, said data stream comprising a data signal per skin pixel area of one or more skin pixels for a plurality of skin pixel areas of said region of interest, a data signal representing the detected electromagnetic radiation reflected from the respective skin pixel area over time. An analyzer analyzes spatial and/or temporal properties of the skin area. A processor determines a vital sign information signal of the subject based on the data signals of skin pixel areas within the skin area, and a post-processor determines the desired vital sign from said vital sign information signal, wherein said determined spatial and/or temporal properties are used by the processor for determining the vital sign information signal and/or by the post-processor for determining the desired vital sign.
    Type: Grant
    Filed: March 7, 2014
    Date of Patent: August 21, 2018
    Assignee: KONINKLIJKE PHILIPS N.V.
    Inventors: Ihor Olehovych Kirenko, Albertus Cornelis Den Brinker
  • Patent number: 10049450
    Abstract: Methods, apparatus, and other embodiments associated with classifying a region of tissue represented in a digitized whole slide image (WSI) using iterative gradient-based quasi-Monte Carlo (QMC) sampling. One example apparatus includes an image acquisition circuit that acquires a WSI of a region of tissue demonstrating cancerous pathology, an adaptive sampling circuit that selects a subset of tiles from the WSI using an iterative QMC Sobol sequence sampling approach, an invasiveness circuit that determines a probability of a presence of invasive pathology in a member of the subset of tiles, a probability map circuit that generates an invasiveness probability map based on the probability, a probability gradient circuit that generates a gradient image based on the invasiveness probability map, and a classification circuit that classifies the region of tissue based on the probability map. A prognosis or treatment plan may be provided based on the classification of the WSI.
    Type: Grant
    Filed: September 30, 2016
    Date of Patent: August 14, 2018
    Assignee: Case Western Reserve University
    Inventors: Anant Madabhushi, Angel Alfonso Cruz Roa, Fabio Gonzalez
  • Patent number: 9983134
    Abstract: In accordance with preferred embodiments of the present invention, a method for imaging tissue, for example, includes the steps of mounting the tissue on a computer controlled stage of a microscope, determining volumetric imaging parameters, directing at least two photons into a region of interest, scanning the region of interest across a portion of the tissue, imaging a plurality of layers of the tissue in a plurality of volumes of the tissue in the region of interest, sectioning the portion of the tissue, capturing the sectioned tissue, and imaging a second plurality of layers of the tissue in a second plurality of volumes of the tissue in the region of interest, and capturing each sectioned tissue, detecting a fluorescence image of the tissue due to said excitation light; and processing three-dimensional data that is collected to create a three-dimensional image of the region of interest.
    Type: Grant
    Filed: June 10, 2014
    Date of Patent: May 29, 2018
    Inventor: Timothy Ragan
  • Patent number: 9977230
    Abstract: A microscope system includes a virtual-slide microscope that acquires a virtual-slide image of a specimen when mounted on a first stage, and a fluorescence microscope that acquires a fluorescence image of the specimen when mounted on a second stage. The virtual-slide microscope includes a display and an image-capturing-condition setting portion that sets image capturing conditions, including a fluorescence-image acquisition position, on the virtual-slide image. The fluorescence microscope includes a memory portion that stores coordinate transformation data between the coordinates of the specimen when fixed on the first stage and the coordinates of the specimen when fixed on the second stage, a condition input portion to which the image capturing conditions are input, and a driving-condition setting portion that sets a driving condition on the basis of the image capturing conditions and the coordinate transformation data.
    Type: Grant
    Filed: November 6, 2015
    Date of Patent: May 22, 2018
    Assignee: OLYMNPUS CORPORATION
    Inventor: Kiyoshi Yuri
  • Patent number: 9970874
    Abstract: Disclosed are methods and systems for analyzing images of specimens processed by a programmable quantitative assay or more specifically a robust programmable quantitative dot assay, PDQA, that enable specimens to be imaged and assessed across a wide variety of conditions and applications. Specific embodiments directed to immunohistochemical applications provide more quantitative methods of imaging and assessing biological samples including tissue samples.
    Type: Grant
    Filed: November 29, 2011
    Date of Patent: May 15, 2018
    Assignee: DAKO DENMARK A/S
    Inventors: Jesper Lohse, Hans Christian Pedersen, Joachim Schmid, Jeff Caron, Rohit Jain, Thomas Briscoe
  • Patent number: 9972097
    Abstract: An image processing device includes a processor to detect motion of objects in an observation area on the basis of a captured image. The processor also calculates amounts of each detected motion, and calculates a propagation speed, a propagation direction, or both, of the motion for pulsations of the objects.
    Type: Grant
    Filed: July 9, 2014
    Date of Patent: May 15, 2018
    Assignee: SONY CORPORATION
    Inventors: Takeshi Kunihiro, Tomohiro Hayakawa, Eriko Matsui, Seiji Kobayashi
  • Patent number: 9953417
    Abstract: A spatial distribution of cells or sub-cellular structures of a subject is quantified by receiving image data that includes a plurality of biomarkers and processing the image data to obtain a set of coordinates. Each coordinate denotes the location of a cell or sub-cellular structure represented by a biomarker or combination of biomarkers. The set of coordinates is processed into a two-dimensional symmetric matrix. Each element of the matrix indicates the frequency of a cell or sub-cellular structure represented by a first biomarker or combination of biomarkers being observed within an interaction distance of a cell or sub-cellular structure represented by a second biomarker or combination of biomarkers. A classifier assigns the subject to a group or category based on the matrix. A toxicity and/or efficacy of one or more interventions is assessed based on a comparison of the spatial distribution to one or more predetermined reference or control distributions.
    Type: Grant
    Filed: October 3, 2014
    Date of Patent: April 24, 2018
    Assignee: The University of Manchester
    Inventors: Richard John Byers, Christopher Rose
  • Patent number: 9951388
    Abstract: In general, the presently disclosed technology relates to identification of cancer subtypes. More specifically, the technology relates to methods for determining molecular drivers of cancer and/or progression using a multivariate image data and statistical analysis of in-situ molecular markers and morphological characteristics in the same cells of a biological sample suspected of b cancer. This analysis takes place after a single acquisition that obtains the molecular and anatomic morphology data in parallel. The analysis compares specific morphological and molecular markers to known samples exhibiting particular genetic drivers of the cancer. This method provides statistical information that allows for an increased confidence in the identification of specific molecular drivers of the cancer.
    Type: Grant
    Filed: May 7, 2012
    Date of Patent: April 24, 2018
    Assignees: Ventana Medical Systems, Inc., Cornell University
    Inventors: Francesca Demichelis, Karl Garsha, Phillip C. Miller, Ray B. Nagle, Michael Otter, Gary Anthony Pestano, Mark Rubin
  • Patent number: 9947163
    Abstract: A method of authenticating security documents and a mobile device, especially a smartphone, programmed to carry out the method, based on an analysis of features which are produced by intaglio printing, which analysis involves a decomposition of sample images of a candidate document to be authenticated based on Wavelets, each sample image being digitally processed by performing a Wavelet transform of the sample image in order to derive a set of classification features. The method is based on an adaptive approach, which includes the following steps: —prior to carrying out the Wavelet transform, defining a categorization map containing local information about different intaglio line structures that are found on the security documents; —carrying out a Wavelet selection amongst a pool of Wavelet types based on the categorization map; and —performing the Wavelet transform of the sample image on the basis of the selected Wavelet.
    Type: Grant
    Filed: February 4, 2014
    Date of Patent: April 17, 2018
    Assignee: KBA-NotaSys SA
    Inventors: Volker Lohweg, Jan Leif Hoffmann, Helene Dörksen, Roland Hildebrand, Eugen Gillich, Jürg Hofmann, Johannes Georg Schaede
  • Patent number: 9943224
    Abstract: This invention provides an image processing apparatus which allows an operator to easily recognize relevance between morbid portions, which exist in a plurality of layers, according to a disease.
    Type: Grant
    Filed: April 25, 2016
    Date of Patent: April 17, 2018
    Assignee: Canon Kabushiki Kaisha
    Inventors: Hiroshi Imamura, Kazuhiro Miyasa
  • Patent number: 9939370
    Abstract: Disclosed herein is a process and system to correct reflective distortions of an optical spectrum. In addition, a spectroscopy system that compensates for reflective distortions is disclosed.
    Type: Grant
    Filed: June 25, 2012
    Date of Patent: April 10, 2018
    Assignee: Northeastern University
    Inventors: Max Diem, Benjamin Bird, Milos Miljkovic
  • Patent number: 9928403
    Abstract: A system and method for analyzing multi-dimensional images includes a high content imaging system that includes an image capture device. An image acquisition module receives a series of images of a biological sample captured by the image capture device, and the series of images includes a sequence of image planes. A human interface module receives from a user computer specifications of a first image analysis step and a second image analysis step. The first image analysis step specifies a first image processing operation that processes an image plane of a series of images in accordance with at least another image plane of the series of images and the second image analysis step specifies a second image processing operation that processes each image plane of a series of images independently of the other image planes of the series.
    Type: Grant
    Filed: February 9, 2016
    Date of Patent: March 27, 2018
    Assignee: Molecular Devices, LLC
    Inventors: Avrum Cohen, Dihui Hong, Steven Luke
  • Patent number: 9910961
    Abstract: Systems and methods are provided to facilitate consultations between a referral source (e.g., labs, pathologists and patients) and a consultant (e.g., pathologist, radiologist, or other digital image analyst). Links between the various referral sources and consultants are established through a scanning center via a data communication network such as the Internet. The referral source sends a slide to the scanning center where the corresponding digital slide is posted for review and analysis by the consultant. Upon completion of the analysis and report, a digital slide conference is conducted through the scanning center that provides a venue for direct communication regarding the consultation. The scanning center may also facilitate payment from the referral source to the consultant.
    Type: Grant
    Filed: October 22, 2013
    Date of Patent: March 6, 2018
    Assignee: Leica Biosystems Imaging, Inc.
    Inventors: Dirk G. Soenksen, Kathy Zirker-Smith, Ole Eichhorn
  • Patent number: 9898842
    Abstract: In one aspect, a method can include a computing system receiving data for rendering as a plurality of objects in a chart, and rendering the plurality of objects in a first order in a first graph in a virtual display. The method can include swapping a first object and a second object in the first order of the plurality of objects creating a second order of the plurality of objects, rendering the objects in a second graph in the virtual display based on the second order, determining that a number of visible objects in the first graph is less than a number of visible objects in the second graph, and outputting the second order of the plurality of objects for rendering on a display device based on determining that a number of visible objects in the first graph is less than a number of visible objects in the second graph.
    Type: Grant
    Filed: March 2, 2015
    Date of Patent: February 20, 2018
    Assignee: SAP SE
    Inventor: Uwe Jugel
  • Patent number: 9894285
    Abstract: Described is system for real-time automated exposure adjustment of a camera using contrast entropy. Multiple images are captured using an image sensor, where each of the images is captured at a distinct exposure value. A contrast entropy is determined for each of the multiple images. An image having the largest contrast entropy is selected among the images and output.
    Type: Grant
    Filed: December 16, 2015
    Date of Patent: February 13, 2018
    Assignee: HRL Laboratories, LLC
    Inventors: Zhichao Chen, Yang Chen, Deepak Khosla, Darrel J. VanBuer
  • Patent number: 9886634
    Abstract: An analytical device is disclosed that analyzes whether a first image is similar to (or the same as) as a second image. The analytical device analyzes the first image by combining at least a part (or all) of the first image with at least a part (or all) of the second image, and by analyzing at least a part (or all) of the combined image. Part or all of the combination may be analyzed with respect to the abstraction of the first image and/or the abstraction of the second image. The abstraction may be based on a Bag of Features (BoF) description, based on a histogram of intensity values, or based on other types of abstraction methodologies. The analysis may involve comparing one or more aspects of the combination (such as the entropy or randomness of the combination) with the one or more aspects of the abstracted first image and/or abstracted second image. Based on the comparison, the analytical device may determine whether the first image is similar to or the same as the second image.
    Type: Grant
    Filed: December 2, 2013
    Date of Patent: February 6, 2018
    Assignee: Sensormatic Electronics, LLC
    Inventors: Kuntal Sengupta, Ian Westmacott, Serdar Ince
  • Patent number: 9881383
    Abstract: A method of accurately projecting a laser image pattern on a work surface and continuously compensating for relative dynamic movement between the laser and the work surface, including establishing a position of the camera in three dimensions relative to the work surface by locating the targets in the camera image, establishing a position of the laser relative to the work surface utilizing the fixed position of the laser relative to the camera, and using a computer to continuously adjust the rotation of the laser projector mirrors in response to dynamic movement of the laser projector relative to the work surface as determined by the camera. In one embodiment, the computer continuously tracks at least two prior locations of the laser projector relative to the work surface and predicts the next location of the laser, compensating for movement of the laser relative to the work surface.
    Type: Grant
    Filed: January 22, 2014
    Date of Patent: January 30, 2018
    Assignee: VIRTEK VISION INTERNATIONAL ULC
    Inventor: Kurt D. Rueb
  • Patent number: 9861609
    Abstract: The present invention provides methods for producing cannabinoids and cannabinoid analogs as well as a system for producing these compounds. The inventive method is directed to contacting a compound according to Formula I or Formula II with a cannabinoid synthase. Also described is a system for producing cannabinoids and cannabinoid analogs by contacting a THCA synthase with a cannabinoid precursor and modifying at least one property of the reaction mixture to influence the quantity formed of a first cannabinoid relative to the quantity formed of a second cannabinoid.
    Type: Grant
    Filed: August 19, 2016
    Date of Patent: January 9, 2018
    Assignee: FULL SPECTRUM LABORATORIES LIMITED
    Inventors: Robert Winnicki, Marc Donsky, Mingyang Sun, Richard Peet
  • Patent number: 9846949
    Abstract: Examples disclosed herein relate to determining the shape of an object representation. In one implementation, a processor determines contours of a silhouette of an object representation above a contour degree threshold, where a contour degree is determined based on the area of the contour with respect to itself. The processor may identify a shape type of the object representation based on a comparison of the determined contours to shape definition information.
    Type: Grant
    Filed: November 27, 2013
    Date of Patent: December 19, 2017
    Assignee: Hewlett-Packard Development Company, L.P.
    Inventor: Nathan Moroney
  • Patent number: 9841380
    Abstract: This invention concerns the field of sample identification, in particular a method and apparatuses for identifying or discriminating biological species from non biological species, both as individual particles and as components of a composition, by pump-probe fluorescence spectroscopy for time-resolved detection or imaging. The method uses the finding that the UV-induced fluorescence of biological molecules is varied, in particular is depleted, by the addition of visible radiation, whereas this does not occur with non-biological organic molecules. The invention discriminates the fluorescence signals of bio and non-bio particles or species using a differential approach, i.e. the comparison. of the total fluorescence recorded with and without additional visible radiation. This allows to discriminate biological particles comprising aromatic amino-acids (AA), like peptides, proteins, bacteria, viruses, pollens, spores, etc.
    Type: Grant
    Filed: August 15, 2016
    Date of Patent: December 12, 2017
    Assignee: University of Geneva
    Inventors: Jean-Pierre Wolf, Luigi Bonacina, Gustavo Alexandre Subtil Sousa
  • Patent number: 9839375
    Abstract: Data derivable from remotely detected electromagnetic radiation (16) emitted or reflected by a subject (12) is processed. The data includes physiological information. An input signal is received and indicative entities are transmitted. The indicative entities being indicative of physiological information representative of at least one vital parameter (17; 150) in a subject (12) of interest, wherein the indicative entities are detected under consideration of at least one defined descriptive model (114) describing a relation between physical skin appearance characteristics and a corresponding representation in the input signal such that non-indicative side information represented by non-indicative entities in the input signal is substantially undetectable in a resulting transmitted signal. The at least one vital parameter (17; 150) is detected from the transmitted signal including the indicative entities.
    Type: Grant
    Filed: September 17, 2013
    Date of Patent: December 12, 2017
    Assignee: KONINKLIJKE PHILIPS N.V.
    Inventors: Caifeng Shan, Ihor Olehovych Kirenko
  • Patent number: 9824446
    Abstract: A computer-based method and system assists users performing medical imaging studies, such as echocardiograms. While the user manipulates the imaging device on an individual, the system automates image processing, relieving the user from the need to simultaneously operate an application and an imaging device. The system assesses an organ system, then through comparison with other imaging data sets, generates an evaluative assessment of the organ system. The system becomes increasingly image-aware and content-aware during an evaluation, and by sequential comparisons against progressive data sets, refines an evaluative assessment.
    Type: Grant
    Filed: March 14, 2014
    Date of Patent: November 21, 2017
    Inventor: Stephanie Littell