Abstract: Methods for production of ethanol, distiller's corn oil, and enhanced co-products are disclosed. Methods include obtaining a mixture of one or more co-products of an alcohol production process, which may include wet cake, performing hydrolysis of polysaccharides in the mixture to generate fermentable sugars, fermenting the fermentable sugars in the mixture to produce alcohol, distilling the mixture to remove alcohol from the mixture thereby producing alcohol-containing distillate and enhanced whole stillage, removing released oil from the fermented mixture before distillation and/or from the enhanced whole stillage after distillation, and recovering enhanced wet distiller's grains, enhanced thin stillage, and/or enhanced dried distiller's grains. Compositions disclosed herein include enhanced dried distiller's grains having a crude protein content of at least 45% on a dry weight basis and having a total fat content of less than 10% on a dry weight basis.

Abstract: The present invention describes a bio-based process to produce high quality protein concentrate (HQPC) by converting plant derived celluloses into bioavailable protein via aerobic incubation, including the use of such HQPC so produced as a nutrient, including use as a fish meal replacement in aquaculture diets.

Abstract: The invention relates to a method for producing a solid transformation product of a substrate comprising the following steps: •preparing a substrate of biomass comprising carbohydrates and proteinaceous matter that originates from soya bean, rape seed, or mixtures thereof, optionally in further mixture with carbohydrates and proteinaceous matter originating from fava beans, peas, sunflower seeds, lupine, cereals, and/or grasses, •mixing said substrate with a live microorganism or a combination of live microorganisms, which live microorganism or mixture of live microorganisms is not live yeast, and adding water in an amount which provides an initial incubation mixture having a water content from 30 to 70% by weight, and a ratio of wet bulk density to dry bulk density from 0.60 to 1.45 in the resulting mixture; •incubating said initial incubation mixture for 1-240 hours at a temperature of 15-70° C.

Abstract: Methods for enhancing phytase thermal stability by fusing binding elements to target phytases are provided. Engineered phytases that include binding elements fused to target phytases to cause cyclization of the engineered phytases and enhance thermal stability of the target phytases are described. Engineered nucleic acids encoding engineered phytases and hosts engineered to express engineered nucleic acids are also provided. Methods for incorporating engineered phytases in animal feed and animal feed including the same are described.

Abstract: This disclosure provides methods for fermentation of sugar substrates by acid-tolerant bacteria for producing acetic and lactic acids, and methods for further processing of these products to obtain acetate and propionate biodegradable deicers. Methods are also disclosed on the use of acid tolerant bacteria to produce acetate and propylene glycol deicing compounds.

Abstract: The present invention describes a bio-based process to produce high quality protein concentrate (HQPC) by converting plant derived celluloses into bioavailable protein via aerobic incubation, including the use of such HQPC so produced as a nutrient, including use as a fish meal replacement in aquaculture diets.

Abstract: Provided a complex bacteria for improving the corn steeping effects and usage thereof. The complex bacteria comprises strains capable of producing protease, cellulase and hemicellulase. Using the complex bacteria to steep corn, effects of reducing the addition amount of SO2, shortening the steeping time and improving the starch yield are achieved.

Abstract: The invention relates to the use of at least one bacterial phytase in combination with one or more protease(s) in animal feed for improving weight gain and/or Feed Conversion Ratio (FCR), wherein the phytase is administered in one or more of the following amounts (dosage ranges): 1,000 FYT/kg feed, 2,000 FYT/kg feed 3,000 FYT/kg feed and wherein the protease is administered in one of the following amounts (dosage ranges): 10,000 units/kg feed, 11,000, 12,000, 13,000, 14,000, 15,000, 16,000, 17,000, 18,000, 19,000, 20,000 units/kg feed.

Abstract: Method for the production of apple sauce including the following steps: slicing apples generating a plurality of slices having two opposite faces and a peel which develops continuously between the two opposite faces; pulping the slices obtaining a first product and a first waste; refining the first waste generating a second product and a second waste; mixing the first product and the second product; heating the combination of the first product and the second product in order to deactivate enzymes contained therein.

Abstract: The present invention relates to ingredients for animal feed compositions for enhancing animal growth and/or animal health. The invention also relates to methods for producing such ingredients and feed compositions. The methods of the invention further allow improving the palatableness and/or digestibility of feed compositions. More specifically, the invention describes the use of a mix of Deinococcus or related bacteria and biomass as a supply of organic constituents in feed compositions.

Abstract: A thermophilic enzymatic biosynthesis (TEBS) device (50) produces outputs of newly synthesized substances, stabilized matter and fully recovered organic material, wherein the preferred device is a dry closet employing multistage treatment of organic solid, liquid and gaseous wastes. Said contemplated device comprises a multiphase thermophilic environment chamber (MTEC) (1) having a mixing zone (4), a cultivation zone (12), a pasteurization zone (24) and a germination zone (7) which utilizes a multiphase germination (62). The device comprises a thermodynamic pathway (29) and a functional respiration (64) which is directed toward an ammine reaction chamber (ARC) (3), which includes an oxidation surface (47) having reactivity with ammonia, producing a metal ammine complex. The device further comprises a subterranean uptake chamber (SUC) (2) which includes a plant growth medium (44) where gases received from the ARC (3) disperse to an uptake root structure (46), thereby reducing carbon dioxide emissions.

Abstract: Biological waste such as food, organic or other biologically-derived waste is converted into shelf-stable and health-safe invertebrate feed. The method for converting includes pre-treating waste by fragmenting, reducing microbial contaminants, optionally amending with components that optimize fermentation, inoculating with microorganisms and mixing. Fermentation takes place in a bioreactor and produces fermentation leachate and solid fermentate. In the post-treatment steps, the solid fermentate is separated from the fermentation leachate. The solid fermentate is ground, dewatered and milled. The solid fermentate can be used as an invertebrate feed with or without further processing.

Abstract: A method for producing aerobic-type single cell protein using the autolysis process, comprising the following steps: precipitation and concentration, centrifugal dewatering, drying, sterilization, smashing and packaging. The materials with moisture content of 78%˜85% after centrifugal dewatering are dried after the autolysis. The present invention and its products employ the autolysis process. During the autolysis, macromolecular nutrients and cell walls are decomposed by autolytic enzymes, to produce high content of free amino acids, free nucleotides; besides, cell wall polysaccharides are decomposed and the cells produce permeability to ensure an active material can flow out of the cell which can be directly absorbed by animals.

Abstract: The present invention provides isolated acidophilic Fusarium oxysporum strains, such as MK7, and their progeny, compositions comprising such strains and their progeny, methods of producing such strains and their progeny, and methods of using such strains and their progeny.

Abstract: The present invention provides a topical formulation including (i) comfrey or a comfrey-derived compound, or an analog or derivative thereof and (ii) a penetration enhancer, and optionally including tannic acid, or an analog or derivative thereof. The present invention also provides a topical formulation including (i) comfrey or a comfrey-derived compound, or an analog or derivative thereof, and (ii) tannic acid, or an analog or derivative thereof, optionally including a penetration enhancer. Also provided are kits and methods of prophylactic or therapeutic treatment of inflammatory and/or degenerative conditions using the combination of active ingredients as herein described.

Abstract: The present technology relates to novel mycotoxin binder and the use in animal feed. The present disclosure relates also to the use of enzymes for improving the mycotoxin binding ability of by-products derived from a fermentative production process and to compositions comprising enzymes capable of degrading components in the fermented mash in the fermentation process.

Abstract: A method for treating highly concentrated resin-desorption liquid generated from wastewater deep purification, including channeling the highly concentrated desorption liquid generated from anion exchange resin treatment through a nanofiltration membrane, the liquid being separated into nanofiltration permeate that can be reused as the desorption agent and highly concentrated nanofiltration retentate; adding a coagulating agent to the highly concentrated nanofiltration retentate to generate coagulation-precipitation; subjecting the supernatant formed after the coagulation-precipitation process to Fenton oxidation or ozone oxidation; after the reaction, adding to the liquid an alkali solution for further coagulation-precipitation; then channeling the liquid so treated back to the biochemical system of biochemical effluent treatment for further biodegradation is provided.

Abstract: The present invention relates to isolated polypeptides having xylanase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.

Abstract: The present invention relates to isolated polypeptides having peroxygenase activity, and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.

Abstract: The present invention relates to separation of desired target products from biological, plant, and waste-type material, wherein the desired target products include renewable fuels such as ethanol, biobutanol, and biodiesel, wherein the separation is conducted with a cross-flow filtration system having the ability to separate desired products from both non-viscous and viscous medium.

Abstract: The present invention relates to a method for detoxification of feed products contaminated by the mycotoxin zearalenone.

Abstract: By combination of hydrophobic chromatography and strongly basic anion-exchange chromatography, a novel, highly hydrophobic ?-glucosidase was successfully identified from Acremonium cellulolyticus. Further, a gene corresponding to the identified ?-glucosidase was isolated. When multiple modifications were introduced into the base sequence of the gene, the gene was successfully expressed in Trichoderma viride at a high level, and the expression product successfully exhibited a high ?-glucosidase activity.

Abstract: The present invention describes fermentation methods for producing animal feeds enriched with omega-3 fatty acids from cellulosic feedstock.

Abstract: The invention relates to an animal feed for dogs or cats based on animal tissue, water, and hydrocolloid as a thickener, wherein the animal feed includes a water content of at least 80% by weight, preferably at least 85% by weight. According to the invention, said feed is liquid at room temperature, has a fat content at least 1% by weight and less than 5% by weight, and is packed in a tubular bag, wherein the top side and the bottom side thereof are welded at a welding surface at a front end and back end of the tubular bag, and a designed fracture point for tearing open the bag is provided at the front end in the welding surface.

Abstract: An asparaginase enzyme derived from the fungi Basidiomycete, in particular the Basidiomycete is Flammulina velutipes. A method for hydrolysing at least one of L-asparagine or L-glutamine. A method for reducing acrylamide formation in a substance comprising L-asparagine is also described.

Abstract: The present invention concerns a method of enhancing milk production by a ruminant that includes providing a feed that contains sorbitol and at least one additional feed component, and orally feeding the feed to the ruminant, the ruminant ingesting about 100 grams, or less, of sorbitol per day.

Abstract: A process is disclosed for obtaining citrus fiber from citrus peel. Citrus fiber is obtained having a c* close packing concentration value of less than 3.8 wt % anhydrous base. The citrus fiber can be used in food products, feed products, beverages, personal care products, pharmaceutical products or detergent products.

Abstract: A method for treatment of a cellulosic material is disclosed. More particularly, the treatment increases the digestibility of cellulosic material following microbial or biological processes.

Abstract: The present invention relates to protein engineering, and concerns especially family G/11 xylanases, and genes encoding said enzymes. In specific, the invention concerns Trichoderma reesei XYNII gene, which codes for endo-1,4-?-xylanase (EC 3.2.1.8). The invention describes how site-directed mutagenesis can be used to improve the properties of an enzyme to match the industrial conditions where it is used. Protein engineering can be used to improve thermoactivity and thermostability of xylanases, as well as to broaden their pH range.

Abstract: Animal feeds comprising fungal mass and proteinaceous material and methods for bioconverting cellulosic materials to produce the same are described. Also described are apparatuses for use in the methods.

Abstract: A method is described for improving the digestibility of a forage diet for ruminant animals. A forage, including alfalfa, Chinese wildrye, corn silage, straw silage, corn stover, ryegrass or TMR, is treated with an enzyme product having cellulase, xylanase, beta-glucanase, pectinase, mannanase and alpha-galactosidase activities.

Abstract: Disclosed is a method for improving the digestibility and amino acid profile of proteinaceous animal feed products by the use of a combination of acidic, alkaline and/or neutral proteases. The selection and amounts of the proteases is based on the particular animal feed product and on the conditions of the gastrointestinal tract of the animal to be fed the treated animal feed. Use of the treated animal feed will reduce the amount of amino acid supplementation required, reduce the amount of nitrogen in animal feces, and improve the economical performance of the animal.

Abstract: Ferulate esterase producing bacterial strains or functional mutants thereof and methods of using ferulate esterase producing bacterial strains as forage additives are disclosed.

Abstract: The invention relates to a novel 3D structure encoding a Nocardiopsis protease, as well as to variants of parent protease homologous to Nocardiopsis proteases, preferably of improved thermostability and/or with an amended temperature activity profile. The invention also relates to DNA sequences encoding such variants, their production in a recombinant host cell, as well as methods of using the variants, in particular within the field of animal feed and detergents. The invention furthermore relates to methods of generating and preparing protease variants of amended properties.

Abstract: Diols produced in fermentation are processed in broth by esterification of the product diol with a carboxylic acid (e.g., fatty acid) and a catalyst (e.g., lipase) capable of esterifying the product diol, such as 1,3-propanediol, with the carboxylic acid to form the diol esters. The diol esters can be extracted from the broth, and the product diol recovered from the diol esters. The carboxylic acid can also serve as an extractant for removal of the diol esters from the fermentation medium.

Abstract: The object of the invention is a new strain of Lactobacillus buchneri A deposited in the Collection of Industrial Microorganisms in the Institute of Agricultural and Food Biotechnology in Warsaw, under the number KKP 2047 p. The invention also concerns composition and a multi-component preparation for starch-rich plant preservation comprising this new strain, uses thereof and the method of silage production or starch-rich plant preservation.

Abstract: This invention provides methods and compositions for producing reduced cholesterol animal foodstuffs and products by feeding livestock and other food-producing animals with feed supplemented with microbial cultures containing hypocholesterolemic compounds produced by microorganisms comprising said microbial cultures. The invention provides low cholesterol poultry, eggs, meat, whole milk, and dairy products.

Abstract: The invention relates to dietary fiber for human and animal nutrition and more particularly to roughage consisting of a fiber formulation containing lignocellulose. Said formulation contains a fraction of a fibrous substance that is fermentable in the digestive tract and a fraction of a fibrous substance that is poorly or non-fermentable in the digestive tract.

Abstract: Systems and methods provide sugar alcohol to heat stressed ruminants to improve performance. During periods of high temperature or humidity, heat stressed ruminants may exhibit decreased dry matter intake, and in response, an effective amount of sugar alcohol such as sorbitol may be provided in the ruminant diet to cause performance to increase, which may include increased milk yield, improved feed efficiency or both compared to heat stressed dairy cattle without sugar alcohol in the diet.

Abstract: The present invention provides an improved method of preparing a fermented proteinaceous livestock feed having improved nutritional value such as increased bioavailability of protein. The invention further provides fermented proteinaceous livestock feed obtained from the method of the invention and uses thereof.

Abstract: The present invention relates to isolated polypeptides having esterase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.

Abstract: In one aspect, the invention is directed to polypeptides having an amylase activity, polynucleotides encoding the polypeptides, and methods for making and using these polynucleotides and polypeptides. The polypeptides of the invention can be used as amylases to catalyze the hydrolysis of starch into sugars.

Abstract: The invention relates to compositions for use as silage inoculants comprising a yeast strain, one or more bacterial stains, and a suitable carrier. The invention also relates to methods of improving livestock animal silage and meat and milk performance of a livestock animal.

Abstract: The invention provides a probiotic composition to alleviate Salmonella infection in farm animals. The composition may comprise at least one of Lactobacillus murinus, Lactobacillus pentosus, Lactobacillus salivarius sub-species salivarius, and Pediococcus pentosaceus. The composition maybe formulated as an animal feedstuff, or as a pharmaceutical composition.

Abstract: Novel fungal endoglucanases Ce15 and Ce112 are disclosed. The endoglucanases are conveniently produced by recombinant technology, and means for their production are described. The endoglucanases are used for treating cellulosic material, especially in textile industry, e.g. in biofinishing or biostoning. They may also be used in detergents, in animal feed and/or in pulp and paper industry, or in hydrolysis of lignocellulosic material for, e.g. bioethanol production.

Abstract: The invention relates to Mutant cellobiohydrolase, being a mutant of SEQ ID NO:1, having a substitution at position N247(I,F,H,W) of SEQ ID NO: 1, wherein the mutant cellobiohydrolase has at least 50% sequence identity with SEQ ID NO: 1, and wherein the mutant cellobiohydrolase has CBHI activity.

Abstract: In one embodiment, the animal feed product of the present invention is devolatilized. In addition, in various embodiments, the animal feed of the present invention is already size-reduced, which allows ease of handling and transport, such as further densification, including pelletization and/or other formatting process for rail transportation. In various embodiments, the animal feed of the present invention has a low sulfur content, high amount of ADF, and/or high amount of starch to meet desired nutritional amounts, which further can allow it to be a substantial or complete replacement of forage. In addition, in certain embodiments, grain is preferably not used in the production of the animal feed, but rather vegetative material is used instead.

Abstract: The present invention relates to protein engineering, and concerns especially family G/11 xylanases, and genes encoding said enzymes. In specific, the invention concerns Trichoderma reesei XYNII gene, which codes for endo-1,4-?-xylanase (EC 3.2.1.8). The invention describes how site-directed mutagenesis can be used to improve the properties of an enzyme to match the industrial conditions where it is used. Protein engineering can be used to improve thermoactivity and thermostability of xylanases, as well as to broaden their pH range.

Abstract: Genetically engineered plants having altered levels of one or more starch regulation enzymes and a polysaccharide degrading enzyme are provided. Methods of genetically engineering plants to express products altering expression of one or more starch regulation enzymes and polysaccharide degrading enzymes, and genetic constructs are provided. Methods of agricultural processing and animal feed using the genetically engineered plants are described.

Abstract: Lignocellulosic biomass is treated to increase accessibility of the material to enzymes and fermentative processes. Accessibility is increased by physical pre-treatment of the biomass using ultrasound and/or microwave and/or cool plasma. The physical treatments degrade the waxy cuticle of the biomass facilitating enzyme accessibility to cellulose and hemicellulose for conversion to utilizable matter, in nutritive and chemical or biofuel industries. These physical treatments improve enzyme accessibility to cellulose and hemicellulose, for enhancing conversion into a range of feed stocks amenable to further processing.