Abstract: A method for producing broiler chickens comprising feeding to the chickens a chicken feed comprising from 103 to 1011 CFU Bacillus bacteria per gram feed, characterized by that the chicken feed has a reduced metabolizable feed energy (ME) level of from 85% to 98% ME of the ME in a standard chicken feed (full ˜100% ME).

Abstract: The invention provides a synthetic phytase polypeptide which encodes an en/ymatically susceptible phytase. Also provided are feed or food products comprising an enzymatically susceptible phytase, and transgenic plants which express the enzymatically susceptible phytase. Further provided are methods for making and using enzymatically susceptible phytases, e.g., a method of using an enzymatically susceptible phytase in feed and food processing.

Abstract: An object is to identify endoglucanase and ?-glucosidase genes by isolating genomic DNA containing cellulase genes, which are classified into endoglucanases or ?-glucosidases, from Acremonium cellulolyticus, and sequencing the nucleotide sequences thereof. The inventors intensively compared the amino acid sequences of known endoglucanases and ?-glucosidases with each other to find conserved region of amino acid sequences in Acremonium cellulolyticus, and various primers were designed based on the information. PCR was carried out using the various primers thus designed and genomic DNA or cDNA as a template. As a result, gene fragments of endoglucanases and ?-glucosidases were obtained. Primers were designed based on the gene fragments, and PCR was carried out to amplify nine genes of endoglucanases and ?-glucosidases. The nucleotide sequences thereof were sequenced, and the present invention was completed.

Abstract: Proteases derived from Nocardiopsis dassonvillei subsp. dassonvillei DSM 43235, Nocardiopsis prasina DSM 15649, Nocardiopsis prasina (previously alba) DSM 14010 Nocardiopsis sp. DSM 16424, Nocardiopsis alkaliphila DSM 44657 and Nocardiopsis lucentensis DSM 44048, as well as homologous proteases; their recombinant production in various hosts, including transgenic plants and non-human animals, and their use in animal feed and detergents. The proteases are acid-stable, alkali-stable, and/or thermostable.

Abstract: The present invention relates to a method for the preparation of 4-hyroxy-L-proline or compositions with a high content of this aminoacid and the use thereof as agrochemical in the agronomical field and/or as a synthesis intermediate of chemical compounds.

Abstract: A method of producing hexose oxidase by recombinant DNA technology, recombinant hexose oxidase and the use of such enzyme, in particular in the manufacturing of food products such as doughs and dairy products, animal feed, pharmaceuticals, cosmetics, dental care products and in the manufacturing of lactones. Suitable sources of DNA coding for the enzyme are marine algal species including Chondrus crispus, Iridophycus flaccidum and Euthora cristata. In useful embodiments, the recombinant hexose oxidase is produced by Pichia pastoris, Saccharomyces cerevisiae or E. coli.

Abstract: The invention relates to products and methods for increasing the productivity in farming by improving the fertility of healthy farming mammals. More in particular, the invention relates to products and methods for improving the fertility of animals by reducing placental insufficiency. According to the invention, this is obtained by providing a product which is enriched in L-arginine. The arginine products of the invention may be applied to improve fertility, which may be used in improving the yield in viable offspring of an animal livestock.

Abstract: A process for extraction of crude oil from DDGS and producing corn distillers meal that may be used as a livestock supplement is disclosed. For example, the corn distillers meal may be used as a crude protein supplement for use in a livestock feed diet or a poultry feed diet.

Abstract: Use of a preparation as an antimicrobial agent or as an anti-oxidant; wherein said preparation comprises a polypeptide having hexose oxidase activity, the polypeptide being in a substantially pure form, and said polypeptide comprises at least one amino acid sequence selected from the group consisting of (i) (SEQ?ID?NO:?1) Tyr-Glu-Pro-Tyr-Gly-Gly-Val-Pro (ii) (SEQ?ID?NO:?2) Ala-Ile-Ile-Asn-Val-Thr-Gly-Leu-Val-Glu-Ser-Gly- Tyr-Asp-X-X-X-Gly-Tyr-X-Val-Ser-Ser- (iii) (SEQ?ID?NO:?3) Asp-Leu-Pro-Met-Ser-Pro-Arg-Gly-Val-Ile-Ala-Ser- Asn-Leu-X-Phe- (iv) (SEQ?ID?NO:?4) Asp-Ser-Glu-Gly-Asn-Asp-Gly-Glu-Leu-Phe-X-Ala-His- Thr (v) (SEQ?ID?NO:?5) Tyr-Tyr-Phe-Lys (vi) (SEQ?ID?NO:?6) Asp-Pro-Gly-Tyr-Ile-Val-Ile-Asp-Val-Asn-Ala-Gly- Thr-X-Asp (vii) (SEQ?ID?NO:?7) Leu-Gln-Tyr-Gln-Thr-Tyr-Trp-Gln-Glu-Glu-Asp (viii) (SEQ?ID?NO:?8) X-Ile-Arg-Asp-Phe-Tyr-Glu-Glu

Abstract: The present invention is directed to novel acid proteases and more specifically to NSP24 family proteases and NSP25 family proteases including biologically active fragments thereof and to nucleic acid molecules encoding said proteases. Also provided are vectors and host cells including nucleic acid sequences coding for the proteases, methods for producing the proteases, enzyme compositions and methods employing said proteases.

Abstract: The present invention provides a means to concentrate, dry and formulate biomaterials such as polysaccharides, gums and related biopolymers, and microorganisms such as cells, spores, and the like from dilute solutions using spent germ and other oil bearing residues. In addition, the spent germ can serve as a carrier for such biomaterials. The sorbed materials are useful in animal feeds.

Abstract: This invention relates to a novel strain of Megasphaera elsdenii and its uses. This invention further relates to preparations and methods incorporating such strain. This invention also relates to feedstuffs for ruminants and a preparation and method for the prevention and treatment of lactic acidosis in ruminants. This invention even further relates to a method of isolating a biologically pure culture of a superior ruminal microorganism in a relatively shorter time period than conventional methods. This invention yet further relates to a method of achieving any one or more of the following improvements in ruminants namely increased milk production; improved feedlot performance; improved growth rate; decrease in finishing time; lower digestive morbidity and mortality; lower incidence of lactic acidosis and related diseases; improved feed conversion efficiency; decrease in roughage content in feeds; and capability to feed on relatively higher concentrate diets.

Abstract: A method of feeding a ruminant that entails providing a feed that comprises sugar alcohol and at least one additional feed component, where the feed is free of rumen-protected fatty acid or comprises less than 10 weight percent rumen-protected fatty acid, based on the total weight of the feed and further entails orally supplying the feed to the ruminant during a pre-partum period when the ruminant is pregnant, where the ruminant ingests the feed and sugar alcohol during the pre-partum period.

Abstract: The present invention relates to isolated polypeptides having acetyl xylan esterase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.

Abstract: The present invention provides compositions and methods designed to increase value output of a fermentation reaction. In particular, the present invention provides a business method of increasing value output of a fermentation plant. The present invention also provides a modified fermentation residual of higher commercial value. Also provided in the present invention are complete animal feeds, nutritional supplements comprising the subject ferment residuals. Further provided by the present invention is a method of performing fermentation, a modified fermentative microorganism and a genetic vehicle for modifying such microorganism.

Abstract: Isolated polypeptide having sialidase activity and having an amino acid sequence which has at least 90% amino acid sequence identity with amino acids 34 to 407 of SEQ ID NO: 3.

Abstract: The invention relates to the abfB-2 gene of Penicillium funiculosum that codes for a type B ?-L-arabinofuranosidase and has a cellulose binding domain. This enzyme ?-L-arabinofuranosidase can be incorporated in nutritional additives or in foods for animals for which it improves the digestibility and thus the nutritional value.

Abstract: A method for the production of ethanol and a modified animal feed is provided. The method replaces the starch in known corn-based animal feed with biomass fiber treated to make it more digestible by animals. The process includes wherein the pericarp and germ are removed from the corn kernel and processed for by-products. The starch and protein are also removed and separated. The starch is then fermented and distilled to ethanol and stillage. The bioavailable modified animal feed comprises the pericarp and germ removed from corn kernels and optionally by-products of the pericarp and germ processing, and lignocellulosic materials. The modified animal feed may optionally include energy materials such as animal and vegetable fats, vegetable soapstocks, or glycerin, and combinations thereof.

Abstract: An animal dietary supplement composition comprises effective amounts of basil and turmeric for tear stain reduction.

Abstract: In a method for treating feed silage for ruminants, by which silage is mixed with a microorganism and the mixture of feed silage and microorganism is stored in a closed container for at least 30 days, it is provided that the feed silage is mixed with at least two microorganisms selected from the group consisting of Enterococcus faecium (DSM 3530), Lactobacillus brevis (DSM 19456), Lactobacillus plantarum (DSM 19457), Lactobacillus kefiri (DSM 19455), Trichosporon spec. nov. (DSM 14153), Trichosporon mucoides (DSM 14156), Trichosporon dulcitum (DSM 14162) and Eubacterium (DSM 11798), as well as an inorganic substance having a large internal surface area. In addition, a feed silage additive for ruminants is provided.

Abstract: This invention relates to newly identified polynucleotides and polypeptides in the phytic acid biosynthetic pathways, variants and derivatives of the same; methods for making the polynucleotides, polypeptides, variants, derivatives and antagonists. In particular, the invention relates to polynucleotides encoding inositol polyphosphate 2-kinase (IPP2-K) and polypeptides exhibiting such activity to modulate the phytic acid biosynthesis in such a way as to decrease phytic acid and/or increase the non-phytic acid phosphorous, especially in corn or soy animal feedstuffs.

Abstract: The invention provides a thermal tolerant (thermostable) cellulase, AviIII, that is a member of the glycoside hydrolase (GH) family. AviIII was isolated and characterized from Acidothermus cellulolyticus, and, like many cellulases, the disclosed polypeptide and/or its derivatives may be useful for the conversion of biomass into biofuels and chemicals.

Abstract: The present invention relates to isolated polypeptides having alpha-glucuronidase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.

Abstract: The invention relates to a novel class of serine proteases of peptidase family S2A or S1E that are stable in the presence of copper (Cu2+) and/or inhibited by copper only to a limited extent. Structural features of potential relevance for this effect are also disclosed. This class of proteases includes proteases derived from Brachysporiella gayana, Nocardiopsis dassonvillei subsp. dassonvillei, Nocardiopsis prasina, and Nocardiopsis alba, but excludes the known proteases derived from Metarhizium anisopliae and Nocardiopsis sp. NRRL 18262. The invention also relates to DNA encoding such proteases, the expression thereof in a host cell, including animal and plant cells, as well as to the use thereof, e.g., in animal feed and in detergents. In particular, the invention relates to animal feed and animal feed additives, such as premix, incorporating these proteases together with 1-500 ppm Cu (in-feed-concentration).

Abstract: The invention relates to a novel 3D structure determined for a Nocardiopsis protease, as well as to variants of parent protease homologous to Nocardiopsis proteases, preferably of improved thermostability and/or with an altered temperature activity profile. The invention also relates to DNA sequences encoding such variants, their production in a recombinant host cell, as well as methods of using the variants, in particular within the field of animal feed and detergents. The invention further relates to methods of generating and preparing protease variants having different properties.

Abstract: The apparatus of the present invention is useful for recycling protein waste and producing fuel from protein waste. Waste is ground by a grinding means and digested by a enzyme digest medium composed of enzymes, preservatives, and inedible egg. The ground proteins are digested with the enzyme in recirculated digest tanks. Fat can be collected from the tank by addition of acid and separation of fat from water with a centrifuge. Alternatively the ground protein and enzyme can be fermented and gas collected from the digest tank in a pressure tank with a compressor. The protein solubles are emulsified, separated from water, and extruded before drying. Either fat or gas can be used to fuel a dryer. The dried proteins are then milled in a hammer mill and screened for uniform size.

Abstract: A process for the conversion of organic materials raw waste and other marine plants and animals into a stable powder form, without high heat or cooking. A raw waste is ground and then optionally hydrolyzed or enzymatically reduced to form a hydrolysate stabilized by adding acid and heated to separate oil and water, to form a product cake transferred to a blender for nutrient mixing, to form a raw product dried in a high velocity air dryer and micronizer. The finely ground raw fish or animal waste, may be cold pressed to remove the oil and water, or bulked with other organic nutrients to adjust pH, increase nutrient value, then blended with nutrients to satisfy feed and fertilizer requirements. The final product may be further milled, classified, and compacted, to control dust and meet market suspension standards for drip, pivot, and other applications for feeding plants and animals.

Abstract: Described herein is a method of preparing DDGS containing reduced levels of beta-glucan and phytic acid suitable for an animal feed.

Abstract: In one aspect, the invention is directed to polypeptides having an amylase activity, polynucleotides encoding the polypeptides, and methods for making and using these polynucleotides and polypeptides. In one aspect, the polypeptides of the invention can be used as amylases, for example, alpha amylases, to catalyze the hydrolysis of starch into sugars. In one aspect, the invention provides delayed release compositions comprising an desired ingredient coated by a latex polymer coating.

Abstract: Novel bacteriocins produced by novel bacterial strains are used for at least reducing the levels of colonization by at least one target bacteria in animals, especially poultry.

Abstract: The present invention relates to a process for producing arachidonic acid. In one embodiment, Mortierella sect. schmuckeri microorganisms are cultured in fermentation medium, preferably containing a component of a complex nitrogen source. Further disclosed is a food product which includes Mortierella sect. schmuckeri microorganisms or lipid isolated from such microorganisms to enhance the arachidonic acid content of the food product.

Abstract: The present invention relates to the construction and engineering of cells, more particularly microorganisms for producing PUFAs with four or more double bonds from non-fatty acid substrates through heterologous expression of an oxygen requiring pathway. The invention especially involves improvement of the PUFA content in the host organism through fermentation optimization, e.g. decreasing the temperature and/or designing an optimal medium, or through improving the flux towards fatty acids by metabolic engineering, e.g. through over-expression of fatty acid synthases, over-expression of other enzymes involved in biosynthesis of the precursors for PUFAs, or codon optimization of the heterologous genes, or expression of heterologous enzymes involved in the biosynthesis of the precursor for PUFAs.

Abstract: Biomass (e.g., plant biomass, animal biomass, microbial, and municipal waste biomass) is processed to produce useful products, such as food products and amino acids.

Abstract: Acetyl esterase producing bacterial strains or functional mutants thereof and methods of using acetyl esterase producing bacterial strains as forage additives are disclosed.

Abstract: A method for producing a feedstuff or feedstuff component, e.g., a palatability-enhancing agent, said method comprising subjecting a microbial culture which comprises a methanotrophic bacterium to autolysis.

Abstract: A method for producing a feedstuff or feedstuff component, e.g. a palatability-enhancing agent, said method comprising subjecting a microbial culture which comprises a methanotrophic bacterium to enzymatic hydrolysis.

Abstract: The present invention relates to codon-optimized xylanase coding sequences and the expression of xylanases in microbes and yeast. The invention further relates to using multiple copies of the xylanase expression construct for high levels of protein expression. The invention also relates to the use of xylanases as feed or food additives. The invention also relates to methods of expression of enzymes to increase thermotolerance by expressing them in organisms that glycosylate proteins compared to expression that the same enzyme without the glycosylation. Further, the invention relates to methods of preparing feed, enzyme feed additives, and methods of reducing the feed conversion ration or increasing weight gain of animals.

Abstract: A method for manufacturing feedstuff including the steps of: preparing wood chips containing large quantities of saw-dusts and/or wood fibers; preparing large quantities of fermentation bacteria, and mixing the wood chips with the large quantities of fermentation bacteria to obtain feedstuff for livestock; the method further including the steps of: preparing ultrafine organic particle substances by ultrafinely shattering waste organic substances such as garbage by using high-speed water flows of 8 m/second or more wherein the ultrafine organic particle substances include those of micrometer sizes created by shearing action of the high-speed water flows, adding the fermentation bacteria to the ultrafine organic particle substances to ferment them and proliferate the fermentation bacteria in great quantities, preparing the fermented ultrafine organic particle substances containing the large quantities of fermentation bacteria, and using the fermented ultrafine organic particle substances as the fermentation bac

Abstract: A source of dietary lipids containing anti-microbial fatty acids is added to a food composition intended for improving or maintaining the skin health and/or coat quality in a pet by preventing or regulating the growth of skin pathogens and of microflora responsible for the generation of body surface and coat odors.

Abstract: The present invention relates to a method for detoxification of feed products contaminated by the mycotoxin aflatoxin.

Abstract: The present invention pertains to the field of liquid feed, and relates to fermented feed products, methods for their preparation, as well as uses thereof. Furthermore, the invention further relates to animal liquid feed and methods of preparing liquid feed using lactic acid bacteria. In particular, a method of preparing a fermented mixed feed is provided, comprising the steps of: (a) providing a liquid fermented product; (b) providing a feed product to be fermented; (c) combining the products from step (a) and (b), and fermenting the feed product of step (b) using the liquid fermented product of step (a) as inoculum.

Abstract: This invention relates to a novel strain of Megasphaera elsdenii and its uses. This invention further relates to preparations and methods incorporating such strain. This invention also relates to feedstuffs for ruminants and a preparation and method for the prevention and treatment of lactic acidosis in ruminants. This invention even further relates to a method of isolating a biologically pure culture of a superior ruminal microorganism in a relatively shorter time period than conventional methods. This invention yet further relates to a method of achieving any one or more of the following improvements in ruminants namely increased milk production; improved feedlot performance; improved growth rate; decrease in finishing time; lower digestive morbidity and mortality; lower incidence of lactic acidosis and related diseases; improved feed conversion efficiency; decrease in roughage content in feeds; and capability to feed on relatively higher concentrate diets.

Abstract: The present invention relates to a method for detoxification of feed products contaminated by the mycotoxin zearalenone.

Abstract: A thermostable glycosidase enzymes derived from various Thermococcus, Staphylothermus and Pyrococcus organisms is disclosed. The enzymes are produced from native or recombinant host cells and can be utilized in the food processing industry, pharmaceutical industry and in the textile industry, detergent industry and in the baking industry.

Abstract: The present invention relates to novel phytase-comprising enzyme granules, the particles of which have a weight-average particle size in the range from 300 to 800 ?m and the specific phytase activity of which, expressed in FTU/g, is at least 13 000 and does not exceed a value of FTUmax=6000[FTU g?1]·D?3·mm3, D being the weight-average particle diameter of the granule particles in mm. The invention further relates to phytase-comprising enzyme granules for feeds, the particles of which have a weight-average particle size in the range from 300 to 800 ?m and the specific phytase activity of which, expressed in FTU/g, is at least 7000, and does not exceed a value of FTUmax=2000[FTU g?1]·D?3·mm3, D being the weight-average particle diameter of the granule particles in mm. The invention also relates to the use of the phytase-comprising enzyme granules in feed compositions and, in particular, pelleted feed compositions, which are obtainable using the phytase-comprising enzyme granules.

Abstract: Novel polypeptides possessing (endo) xylanase activity are disclosed which can degrade cellulose in plant extracts and plant materials. The polypeptides can cleave ?-D-xylan polymers at internal (1-4) bonds between adjacent xylopyranosyl units. The amino acid sequence and encoding DNA sequence is given and the polypeptide was used to treat cellulose in the preparation of edible foodstuffs and animal feed. The polypeptides have both arabinoxylanase and xylosidase activity.

Abstract: The present invention provides improved chlortetracycline-containing animal feed compositions and processes and apparatuses for their preparation. In certain embodiments, raw fermentation broth comprising chlortetracycline is divided into two portions. The first portion is mixed with a compound that complexes chlortetracycline. The second portion is acidified and the solids are removed. The acidified liquid is treated with a complexing agent to produced a chlortetracycline complex. The first and second portions thus treated are then mixed and the mixture is passed on to a filter press or other means for separation of the solids to produce a wet cake comprising complexed chlortetracycline. In alternative embodiments, the second portion may be acidified and filtered and admixed with the first portion prior to the complexing step. The resulting mixture is passed on to a filter press or other means for separation of the solids.

Abstract: A method for the production of ethanol and a modified animal feed is provided. The method replaces the starch in known corn-based animal feed with biomass fiber treated to make it more digestible by animals. The process includes wherein the pericarp and germ are removed from the corn kernel and processed for by-products. The starch and protein are also removed and separated. The starch is then fermented and distilled to ethanol and stillage. The bioavailable modified animal feed comprises the pericarp and germ removed from corn kernels and optionally by-products of the pericarp and germ processing, and lignocellulosic materials. The modified animal feed may optionally include energy materials such as animal and vegetable fats, vegetable soapstocks, or glycerin, and combinations thereof.

Abstract: A secreted proteolytic polypeptide comprising at least three non-polar or uncharged polar amino acids within the last four amino acids of the C-terminus of the polypeptide, encoding polynucleotides, expression vectors comprising the polynucleotides, host cell comprising the polynucleotides, methods for producing said polypeptide, and methods for using the polypeptide.

Abstract: A method for treating silage to enhance aerobic stability by inhibiting growth of microorganisms selected from yeasts, molds and spore-forming bacteria is disclosed. The method comprises treating silage or feed with a composition comprising Lactobacillus buchneri, LN1284, or the antimicrobial components produced thereby. The strain of Lactobacillus buchneri disclosed in the invention has been purified and isolated and has been found to be nontoxic, safe and able to improve aerobic stability of silage.