Differentiated Tissue Or Organ Other Than Blood, Per Se, Or Differentiated Tissue Or Organ Maintaining; Composition Therefor Patents (Class 435/1.1)
  • Patent number: 11313772
    Abstract: Disclosed herein are compositions for fixing tissue for cytologic, histomorphologic, and/or molecular analysis (e.g., DNA, RNA, and/or protein analysis). In some embodiments, the fixatives are aldehyde-free fixatives, for example, formaldehyde- or formalin-free fixatives. Particular disclosed compositions include buffered ethanol. The buffer is a phosphate buffer or phosphate buffered saline (PBS) in some examples. In further embodiments, the fixative includes additional components, such as glycerol and/or acetic acid.
    Type: Grant
    Filed: November 11, 2016
    Date of Patent: April 26, 2022
    Assignee: The United States of America, as represented by the Secretary, Department of Health and Human Services
    Inventors: Stephen M. Hewitt, Joon-Yong Chung, Candice D. Perry, Robert A. Star
  • Patent number: 11214770
    Abstract: The invention relates to a method for polar body injection, which comprises removing a polar body from a first egg cell and injecting the polar body into a second egg cell that is in a fertilizable state.
    Type: Grant
    Filed: September 22, 2016
    Date of Patent: January 4, 2022
    Inventor: Wolfgang Würfel
  • Patent number: 11213026
    Abstract: This invention relates to an aqueous solution for preserving and rinsing organs to be transplanted comprising: sodium (Na+) ions at a concentration between 30 and 150 mmol·L?1; potassium ions (K+) at a concentration between 10 and 40 mmol·L?1; polyethylene glycol with a molecular weight of 35,000 g·mol-1 (PEG 35000) at a concentration between 2 and 5 g·L?1.
    Type: Grant
    Filed: October 30, 2019
    Date of Patent: January 4, 2022
    Inventors: Georges Antoine Lopez, Alexandre Lopez
  • Patent number: 11172674
    Abstract: There is described a preservation solution for the preservation of cells, tissues and/or organs, said solution comprising: (i) water for injection; (ii) at least one saccharide; (iii) at least one component with pH buffer properties; (iv) optionally at least one component with calcium transport blocking properties or an anti-calcium action activity; (v) salicylic acid, in free form or in salt form, or aspirin; and (vi) glutamic acid, in free form or in salt form, or glutamine; provided that acetamide is absent and/or if aspirin is present glutamine is absent and if glutamine is present aspirin is absent.
    Type: Grant
    Filed: July 13, 2018
    Date of Patent: November 16, 2021
    Inventors: Terence Simon Corbitt, Malgosia Jarmolowicz, Clare Lankester, Jeremy Peter Alan Lodge, Oluseun Olarewaju, David John Potts
  • Patent number: 11141398
    Abstract: The present disclosures provides methods for decreasing injuries associated with intraoperative hypotension by intravenously administering to a subject a therapeutically effective amount of a fat emulsion, following a period of intraoperative hypotension and after the subject's mean arterial blood pressure has recovered. The disclosure also provides methods for preventing injuries associated with intraoperative hypotension, particularly for surgical candidates that have an increased risk for intraoperative hypotension. Non-limiting examples of injuries contemplated herein include myocardial injury, myocardial infarction, and acute kidney injury.
    Type: Grant
    Filed: January 5, 2018
    Date of Patent: October 12, 2021
    Assignee: The United States Government as Represented by the Department of Veterans Affairs
    Inventor: Guy Weinberg
  • Patent number: 11116204
    Abstract: A protocol for the cryopreservation of high viscosity biological samples, preferably of spermatic samples of crustaceans or insects, that enables maintaining intact the physical properties of the original sample preserving the viability of the sperm packet.
    Type: Grant
    Filed: September 18, 2017
    Date of Patent: September 14, 2021
    Inventors: Karina Morales Ueno, Carmen Guadalupe Paniagua Chávez
  • Patent number: 11110119
    Abstract: The invention contemplates a copolymer which is a graft or block copolymer useful to change wettability and surface characteristics of biological surfaces. Methods for use of these formulations and coatings to change wettability and sterically stabilize, and lubricate biological surfaces in a subject, for example, in the treatment of dry eye syndrome, and to prevent adherence of unwanted proteins, for example in the treatment of contact lens intolerance, are provided.
    Type: Grant
    Filed: July 27, 2020
    Date of Patent: September 7, 2021
    Assignee: Calm Water Therapeutics LLC
    Inventors: Eugene Rex Cooper, David Maxwell Kleinman, Andrew Loxley, Mark A. Mitchnick
  • Patent number: 11103500
    Abstract: The embodiments described herein include methods and formulations for treating lung and brain injury. The methods and formulations include, but are not limited to, methods and formulations for delivering effective concentrations of levocetirizine and montelukast to a patient in need. The methods and formulations can comprise conventional and/or modified-release elements, providing for drug delivery to the patient.
    Type: Grant
    Filed: February 28, 2018
    Date of Patent: August 31, 2021
    Assignee: IRR, Inc.
    Inventor: Bruce Chandler May
  • Patent number: 11077147
    Abstract: A biological composition has a mixture of mechanically selected allogeneic biologic material derived from placental tissue. The mixture has non-whole cellular components including vesicular components and active and inactive components of biological activity, cell fragments, cellular excretions, cellular derivatives, and extracellular components. The mixture including non-whole cell fractions including one or more of exosomes, transcriptosomes, proteasomes, membrane rafts, lipid rafts. The mixture is compatible with biologic function.
    Type: Grant
    Filed: July 27, 2015
    Date of Patent: August 3, 2021
    Assignee: Vivex Biologics Group, Inc.
    Inventors: Timothy Ganey, Wendy W. Weston, Gaëtan Jean-Robert Delcroix
  • Patent number: 11065031
    Abstract: Systems and methods for assembling a plurality of tissue grafts are provided. A method includes harvesting the plurality of micro tissue grafts from a donor site, arranging the plurality of micro tissue grafts in a desired orientation, forming a tissue construct containing the plurality of micro tissue grafts arranged in the desired orientation, and applying the tissue construct to a recipient site.
    Type: Grant
    Filed: October 30, 2017
    Date of Patent: July 20, 2021
    Assignee: The General Hospital Corporation
    Inventors: Richard Rox Anderson, Joshua Tam, Walfre Franco, William Farinelli, Ying Wang, Martin Purschke
  • Patent number: 11058106
    Abstract: Disclosed herein are compositions, devices, and methods that provide cellular materials in ready to use formats for experimental, therapeutic, and tissue engineering protocols. For example, compositions containing frozen or non-frozen cell present as aggregates are disclosed. Also disclosed a compositions containing cellular materials that after storage are suitable for dispersion, e.g. by 3D printing. Also disclosed is a kit for producing bioink compositions. Also disclosed is a closed system device comprising a cell material composition described herein.
    Type: Grant
    Filed: May 12, 2015
    Date of Patent: July 13, 2021
    Assignee: ROOSTERBIO, INC.
    Inventors: Jonathan Allen Rowley, Lye Theng Lock
  • Patent number: 11028363
    Abstract: Subpopulations of spore-like cells expressing specific cell surface and gene expression markers are provided. In one embodiment, the cells express at least one cell surface or gene expression marker selected from the group consisting of Oct4, nanog, Zfp296, cripto, Gdf3, UtF1, Ecat1, Esg1, Sox2, Pax6, nestin, SCA-1, CD29, CD34, CD90, B1 integrin, cKit, SP-C, CC10, SF1, DAX1, and SCG10. Also provided are methods for purifying a subpopulation of spore-like cells of interest from a population of spore-like cells, and methods for inducing differentiation of the isolated spore-like cells into cells of endodermal, mesodermal or ectodermal origin. The spore-like cells can be used to generate cells originating from all three germ layers and can be used to treat a patient who has a deficiency of functional cells in any of a wide variety of tissues, including the retina, intestine, bladder, kidney, liver, lung, nervous system, or endocrine system.
    Type: Grant
    Filed: June 18, 2018
    Date of Patent: June 8, 2021
    Inventors: Martin P. Vacanti, Charles A. Vacanti
  • Patent number: 10934342
    Abstract: The invention comprises several embodiments of a multi-step method for extracting mineral and protein components from a mineralized biocomposite material, including at least the steps of: extracting proteins and minerals from a material into a first solution by contacting or immersing it with the first solution, comprising one or more of a demineralization solution and a salt solution; obtaining a first solid portion from the first solution, by separating the solid and liquid portions; extracting proteins and minerals from the first solid portion into a second solution by contacting or immersing it with the second solution, comprising one or more of a demineralization solution and a salt solution; and obtaining a second solid portion from the second solution by separating the liquid and solid portions. Each of these embodiments may be operated in succession to any of the other embodiments to create custom optimized extraction processes.
    Type: Grant
    Filed: November 15, 2018
    Date of Patent: March 2, 2021
    Inventor: Atharva Poundarik
  • Patent number: 10925997
    Abstract: A bone bioactive composition and kits comprising the composition, means for applying it and/or a metal implant are provided. The bone bioactive composition comprises a water-based salt solution comprising sodium dihydrogen phosphate and sodium chloride, and may also comprise additional elements. The composition and the kits are useful for promoting osteogenesis, particularly when a metal implant is used but also in case of periodontal diseases.
    Type: Grant
    Filed: October 26, 2017
    Date of Patent: February 23, 2021
    Inventors: Maher Al Atari Abouasi, José Luis Calvo Guirado
  • Patent number: 10925928
    Abstract: A method of ameliorating, inhibiting, preventing, or reducing male infertility in a subject undergoing or in need of chemotherapy by administering to the subject a granulocyte colony-stimulating factor in a protective amount, prior to, during or after administration of one or more chemotherapeutic agents to the subject.
    Type: Grant
    Filed: December 2, 2016
    Date of Patent: February 23, 2021
    Inventor: Brian P. Hermann
  • Patent number: 10898609
    Abstract: Provided herein are methods of producing an acellular tissue product wherein the method can include the step of inducing apoptosis and washing the tissue after induction of apoptosis with a tonic solution. Also provided herein are acellular tissue products produced by the methods provided herein and methods of administering the acellular tissue products to a subject in need thereof.
    Type: Grant
    Filed: July 14, 2016
    Date of Patent: January 26, 2021
    Assignee: University of Florida Research Foundation, Inc.
    Inventors: Christine E. Schmidt, Rebecca Ann Wachs, Robert Chase Cornelison
  • Patent number: 10897891
    Abstract: Provided in some aspects are methods and compositions for preserving platelets or other cells. In some embodiments, a platelet storage media or cell storage media may comprise a low molecular weight polyethylene glycol (e.g. PEG-400) to allow for extended storage and/or refrigeration of the platelets or cells.
    Type: Grant
    Filed: April 8, 2016
    Date of Patent: January 26, 2021
    Assignee: Board of Regents, The University of Texas System
    Inventors: Jeffrey Tarrand, Borje Andersson
  • Patent number: 10874098
    Abstract: An aqueous organ preservation solution includes taurine and L-alanine-L-glutamine and glutamic acid. The organ preservation composition can be stored in a solid state, for example in the form of small particles (e.g. a powder or micronized powder), and be dissolved in water, thereby instantly providing a ready-to-use organ preservation solution.
    Type: Grant
    Filed: April 1, 2016
    Date of Patent: December 29, 2020
    Assignee: TX Innovations B.V.
    Inventor: Benedict Marie Doorschodt
  • Patent number: 10834917
    Abstract: Broadly speaking, embodiments of the present technique provide apparatus for controlling ice formation, such as during the processes of cryopreservation and freeze drying. In particular, the apparatus provides a mechanism to deliver an ice nucleating material into a biological sample that is to be preserved, without allowing the ice nucleating agent to contact or contaminate the biological matter within the sample.
    Type: Grant
    Filed: April 15, 2016
    Date of Patent: November 17, 2020
    Assignee: Asymptote Ltd.
    Inventors: George John Morris, Stephan James Lamb
  • Patent number: 10829515
    Abstract: The present invention relates in a first aspect to a method for the purification of biological macromolecular complexes. Typically, no chromatography steps are applied. That is, the present invention relates to a method for the purification of biological macromolecular complexes Furthermore, the present invention relates to a method for crystallization of biological macromolecular complexes comprising the step of purification as described followed by crystallization in a reservoir solution containing a water-soluble polymer. Furthermore, purified biological macromolecular complexes obtainable by the method according to the present invention are provided as well as crystallized biological macromolecular complexes. Finally, a method for determining the suitability of a candidate compound for inhibiting the 20S proteasome of an individual is provided.
    Type: Grant
    Filed: June 6, 2017
    Date of Patent: November 10, 2020
    Inventors: Ashwin Chari, Holger Stark, Jil Schrader, Fabian Henneberg
  • Patent number: 10795969
    Abstract: An automated life science laboratory or a storage facility for biological specimens may be located together with or in close proximity to a data center. The location of the data center, the automated life science laboratory, and the storage facility may be a location in which land and/or electricity are less expensive than locations where the biological specimens are collected. The automated life science laboratory may have a high-capacity data connection to the data center. The life science laboratory, storage facility, and the data center may share a connection to the electrical grid, an HVAC system, and/or a security perimeter. A biological specimen may be removed from storage at the storage facility, process by one or more biotechnology protocols at the automated life science laboratory, and data from the processing may be stored in the data center.
    Type: Grant
    Filed: May 20, 2016
    Date of Patent: October 6, 2020
    Assignee: Microsoft Technology Licensing, LLC
    Inventors: David E. Heckerman, David H. Shute
  • Patent number: 10779529
    Abstract: Blood and platelet storage and rejuvenating compositions that include triciribine, triciribine metabolites, triciribine analogs, and mixtures of the same are disclosed. Such compositions can be useful in methods for treating (e.g., storing and rejuvenating) red blood cells and platelets.
    Type: Grant
    Filed: August 16, 2019
    Date of Patent: September 22, 2020
    Assignee: RythRx Therapeutics, LLC
    Inventor: Narayanan Surendran
  • Patent number: 10780197
    Abstract: A bone graft composition comprising a viable, osteogenic cellular material combined with a viscous cryoprotectant that includes a penetrating cryoprotective agent and a non-penetrating cryoprotective agent. The viscosity of the cryoprotectant is such that the composition is malleable, cohesive and capable of being formed into desired shapes.
    Type: Grant
    Filed: October 29, 2013
    Date of Patent: September 22, 2020
    Assignee: NuVasive, Inc.
    Inventors: Gregory Williams, Erik Erbe, Susan Riley, Timothy Moseley, Ali Ismailoglu
  • Patent number: 10783803
    Abstract: The presently disclosed subject provides a biomimetic eye model, and methods of its production and use. In one exemplary embodiment, the biomimetic eye model can include a scaffold having a convex curvature, a fluidic device coupled to the scaffold, a fabricated eyelid coupled to the fluidic device and a motor coupled to the fabricated eyelid. In certain embodiments, the scaffold can be impregnated with one or more keratocytes and a surface of the convex curvature of the scaffold can be covered with one or more layers of epithelial cells.
    Type: Grant
    Filed: July 11, 2019
    Date of Patent: September 22, 2020
    Inventors: Dongeun Huh, Jeongyun Seo, Mina Massaro-Giordano
  • Patent number: 10765111
    Abstract: Compositions for maintaining cellular integrity especially in cases of cryopreservation and multiple freeze/thawing cycles are provided. In addition, the compositions can be used in various media for a variety of applications.
    Type: Grant
    Filed: March 4, 2015
    Date of Patent: September 8, 2020
    Assignee: Akron Biotechnology, LLC
    Inventor: Claudia Zylberberg
  • Patent number: 10758336
    Abstract: Disclosed herein are muscle implants and methods of making muscle implants comprising one or more decellularized muscle matrices. The muscle matrices can, optionally, be joined to one or more decellularized dermal matrices. The muscle implants can be used to enhance muscle volume or to treat muscle damage, defects, and/or disorders. The decellularized muscle matrices in the implants retain at least some of the myofibers found in a muscle tissue prior to processing.
    Type: Grant
    Filed: July 1, 2013
    Date of Patent: September 1, 2020
    Assignee: LifeCell Corporation
    Inventors: Hui Xu, Hua Wan, I-Chien Liao
  • Patent number: 10746633
    Abstract: Provided is a tissue slicing device having a first motor, a filling assembly, a filter, a pipe, and a cutter. The filling assembly includes a tube and a piston rod movably inserted through the tube. One end of the piston rod abuts against the first motor. The filter is located at an end of the tube opposite the first motor. The pipe is located at a common side of the first motor and the filling assembly, and includes a nozzle located at an end of the pipe and proximal to an end of the filter opposite the filling assembly. The cutter is proximal to the filter. The guiding body is located at the end of the tube opposite the first motor. Provided is an apparatus having the tissue slicing device, such that manpower and time can be saved, and the tissue slicing degree can be unified.
    Type: Grant
    Filed: March 31, 2017
    Date of Patent: August 18, 2020
    Assignee: SCL Biotech Ltd.
    Inventors: Hsin-Wu Mi, Ming-Cheng Lee
  • Patent number: 10736314
    Abstract: The invention provides, in various embodiments, systems, devices and methods relating to ex-vivo organ care. In certain embodiments, the invention relates to maintaining an organ ex-vivo at near-physiologic conditions. The present application describes a method for using lactate measurement in the arterial and the venous blood lines of the Organ Care System Heart perfusion device to evaluate the: 1) The overall perfusion status of an isolated heart and 2) The metabolic status of an isolated heart and 3) the overall vascular patency of an isolated donor heart. This aspect of the present invention uses the property of myocardial cell's unique ability to produce/generate lactate when they are starved for oxygen and metabolize/utilize lactate for energy production when they are well perfused with oxygen.
    Type: Grant
    Filed: April 26, 2019
    Date of Patent: August 11, 2020
    Assignee: TRANSMEDICS, INC.
    Inventors: Waleed H. Hassanein, Tamer I. Khayal, Ahmed Elbetanony, Paul Lezberg, Giovanni Cecere, Dennis Sousa, Elizabeth (Hansen) Bulger
  • Patent number: 10725043
    Abstract: Provided are a three-dimensional (3D) fibroblast cluster, a method of preparing the same, an in vitro 3D skin dermis model including a fibroblast cluster cultured from a fibroblast, and a method of screening a drug by using the in vitro 3D skin dermis model.
    Type: Grant
    Filed: April 19, 2016
    Date of Patent: July 28, 2020
    Assignee: S-BIOMEDICS
    Inventors: Sang Heon Kim, Jong Hoon Choi, Kwi Deok Park
  • Patent number: 10711239
    Abstract: A centrifuge device is provided for the sizing and separation of constituents of a biologic mixture, e.g., adipose tissue. The device provides for the mechanical breaking down of the fibrous structure in the tissue by centrifugation causing the tissue to pass through a mesh element, or a sizing helix, or an extrusion element, whereupon the material is reduced to a slurry. This processed material may then be separated by centrifugation into its constituents, in order to harvest the fraction containing the multipotent cells. These multipotent cells may be utilized for various medical procedures to stimulate healing and tissue regeneration.
    Type: Grant
    Filed: April 10, 2018
    Date of Patent: July 14, 2020
    Assignee: DSM IP ASSETS B.V.
    Inventors: John E. Nash, William T. Fisher, Gino Bradica
  • Patent number: 10704024
    Abstract: An object of the present invention is to provide a method of simply and efficiently promoting expression of c-MPL on a surface of a mesenchymal cell. The present invention provides a method for producing a mesenchymal cell with promoted expression of a c-MPL receptor on the cell surface, comprising Step A of culturing the mesenchymal cell in a mesenchymal cell culturing basic medium comprising a c-MPL receptor active substance; and Step B of obtaining the mesenchymal cell with promoted expression of a c-MPL receptor on the cell surface.
    Type: Grant
    Filed: August 5, 2016
    Date of Patent: July 7, 2020
    Assignee: Adiposeeds, Inc.
    Inventors: Yumiko Matsubara, Yasuo Ikeda, Yukako Uruga
  • Patent number: 10695407
    Abstract: The present invention relates to a protective solution for preventing or reducing reperfusion injury of the brain which contains magnesium ions and which has an osmolality of 350 to 600 mOsm/L, a pH value of 6.8 to 7.8 and albumin in an amount of 1 to 20% by weight.
    Type: Grant
    Filed: July 1, 2014
    Date of Patent: June 30, 2020
    Assignee: ResuSciTec GmbH
    Inventors: Christoph Benk, Friedhelm Beyersdorf, Georg Trummer
  • Patent number: 10695157
    Abstract: A tissue graft packaging system having a retainer including first and second members with respective first and second pluralities of channel sidewalls that define first and second pluralities of channels, and first and second engagement means configured to removeably interconnect the first and second members. The first and second pluralities of channel sidewalls are configured to create a continuous interior space between the first and second members. A tissue graft, such as a cryopreserved viable tissue graft, is contained within the continuous interior space of the retainer. Processes for packaging, thawing and rinsing the tissue graft using the tissue graft packaging system and its retainer are also disclosed.
    Type: Grant
    Filed: January 10, 2017
    Date of Patent: June 30, 2020
    Assignee: Musculoskeletal Transplant Foundation
    Inventors: Elizabeth Ann Poyss, Todd J. Nilsen, Alison Ling, Kevin Wu
  • Patent number: 10694739
    Abstract: The present invention provides peptoid polymers capable of reducing or inhibiting the formation of ice crystals at sub 0° C. temperatures. Also provided are peptoid-peptide hybrids comprising the peptoid polymers provided herein. The peptoid polymers and peptoid-peptide hybrids provided herein are useful for making cryoprotectant solutions. The peptoid polymers, peptoid-peptide hybrids, and cryoprotectant solutions provided herein are useful for making antifreeze solutions, frozen food products, and cosmetic care products. Also provided herein are methods for preserving a tissue, an organ, a cell, or a biological macromolecule using the compositions described herein.
    Type: Grant
    Filed: May 2, 2018
    Date of Patent: June 30, 2020
    Assignee: X-THERMA, INC.
    Inventor: Xiaoxi Wei
  • Patent number: 10696951
    Abstract: Provided is a method for efficiently culturing pluripotent stem cells with higher safety. The present invention relates to a method for culturing pluripotent stem cells, the method comprising culturing an isolated pluripotent stem cells in a pseudo-microgravity environment to proliferate the pluripotent stem cells while maintaining the pluripotent stem cells in an undifferentiated state, thereby forming and growing spheroids of the pluripotent stem cells; and a method for inducing differentiation of pluripotent stem cells by using the method.
    Type: Grant
    Filed: September 30, 2015
    Date of Patent: June 30, 2020
    Assignee: JTEC Corporation
    Inventors: Toshimasa Uemura, Yui Onomura, Takashi Tsumura
  • Patent number: 10683121
    Abstract: There is provided a method or improving the surface sterility of an invertebrate organism having an external cuticle, comprising contacting an outer surface of the organism, or a portion thereof, with an aqueous alcohol solution of less than about 70% v/v for a period of less than 60 seconds. The method is especially useful to provide research-grade organisms which can be utilised in research involving injection of compositions through the external cuticle, by reducing phenotype changes resulting from introduction of surface contaminants into the interior of the organism.
    Type: Grant
    Filed: August 17, 2016
    Date of Patent: June 16, 2020
    Inventors: Olivia Champion, Richard Titball
  • Patent number: 10675329
    Abstract: The invention features compositions featuring (a) one or more of connective tissue growth factor (CTGF) and human C-terminal CTGF peptide; and (b) one or more of insulin and IGF-1; and methods of using such compositions to reduce cardiac tissue damage associated with an ischemic event or to enhance engraftment of a cell in a cardiac tissue.
    Type: Grant
    Filed: June 14, 2017
    Date of Patent: June 9, 2020
    Assignee: University of Vermont and State Agriculture College
    Inventor: Jeffrey Spees
  • Patent number: 10633623
    Abstract: The presently disclosed subject matter provides a microfluidic device that can simulate capillary blood flow on a fetal side of the device and pooled blood on a maternal side of the device (i.e., intervillous space). The microfluidic device can reconstitute the maternal-fetal interface, can expand the capabilities of cell culture models, and can provide an alternative to current maternal-fetal transfer models.
    Type: Grant
    Filed: March 23, 2016
    Date of Patent: April 28, 2020
    Inventors: Dongeun Huh, Cassidy Blundell
  • Patent number: 10617790
    Abstract: The growth factor profile, connective tissue matrix constituents, and immunoprivileged status of urodele extracellular matrix (ECM) and accompanying cutaneous tissue, plus the presence of antimicrobial peptides there, render urodele-derived tissue an ideal source for biological scaffolds for xenotransplantation. In particular, a biological scaffold biomaterial can be obtained by a process that entails (A) obtaining a tissue sample from a urodele, where the tissue comprises ECM, inclusive of the basement membrane, and (B) subjecting the tissue sample to a decellularization process that maintains the structural and functional integrity of the extracellular matrix, by virtue of retaining its fibrous and non-fibrous proteins, glycoaminoglycans (GAGs) and proteoglycans, while removing sufficient cellular components of the sample to reduce or eliminate antigenicity and immunogenicity for xenograft purposes.
    Type: Grant
    Filed: January 9, 2014
    Date of Patent: April 14, 2020
    Assignee: ISE Professional Testing & Consulting Services, Inc.
    Inventor: Ryanne Early
  • Patent number: 10617745
    Abstract: A method for preventing bleeding, blood-brain barrier disruption, brain injury, or a combination thereof in a subject destined for cardiac surgery is disclosed. The method involves administering to the subject prior to cardiac surgery a therapeutically effective amount of alpha-1 antitrypsin (AAT-1), or functional variant thereof. The result is that it prevents bleeding, blood-brain barrier disruption, or a combination thereof in a subject destined for cardiac surgery.
    Type: Grant
    Filed: March 21, 2018
    Date of Patent: April 14, 2020
    Assignee: Mor Research Applications Ltd.
    Inventor: Dan Abrahamov
  • Patent number: 10575515
    Abstract: The present invention generally relates to methods and compositions to determine viability of an organ for transplantation and other medical purposes. One aspect of the invention relates to a method for assessing the viability of an organ by measuring the energy parameters to determine the energy level of the organ by determining the stored cellular energy (e.g., ATP levels), and/or energy consumption over a particular time period of viability. The energy parameters can be compared to reference energy parameters as a highly accurate and reliable prediction of viable cell yield, and organ viability. Another aspect of the invention relates methods to preserve or extend the time period of viability of an organ any combination of (i) preservation perfusion of the organ to prevent ischemic damage, (ii) chemical metabolic suppression of the organ e.g., using metabolic suppressants, (iii) metabolic suppression by physical or environmental conditions, e.g., sub-zero non-freezing storage.
    Type: Grant
    Filed: May 4, 2011
    Date of Patent: March 3, 2020
    Assignee: The General Hospital Corporation
    Inventors: Martin L. Yarmush, Mehmet Toner, Maria-Louisa Izamis, Timothy Antonie Berendsen, Robert Marius Bieganski, Osman Berk Usta, Basak Elif Uygun, Mustafa Korkut Uygun, Sinem Perk
  • Patent number: 10575516
    Abstract: Disclosed are products having animal tissue packed within the lumen of a device such as a needle cannula and being therein impregnated with a cryopreservation medium. The needle cannula or other device can be received in a capsule and/or other container, which in some forms can contain additional amounts of the cryopreservation medium occurring external of the lumen. Methods of use of the products are also described and can include ejecting the animal tissue from the lumen of device using pressurized liquid passed through the lumen, potentially to deliver the animal tissue directly into a patient. Methods of manufacture of the products are also described.
    Type: Grant
    Filed: November 14, 2018
    Date of Patent: March 3, 2020
    Assignee: Cook Biotech Incorporated
    Inventors: Umesh H. Patel, Michael L. Taylor, Claus Sondergaard, Cara McCammon, Rae Ritchie
  • Patent number: 10579755
    Abstract: A method for producing bone grafts using 3-D printing is employed using a 3-D image of a graft location to produce a 3-D model of the graft. This is printed using a 3-D printer and a printing medium that produces a porous, biocompatible, biodegradable material that is conducive to osteoinduction. For example, the printing medium may be PCL, PLLA, PGLA, or another approved biocompatible polymer. In addition such a method may be useful for cosmetic surgeries, reconstructive surgeries, and various techniques required by such procedures. Once the graft is placed, natural bone gradually replaces the graft.
    Type: Grant
    Filed: April 26, 2019
    Date of Patent: March 3, 2020
    Inventors: Arthur Greyf, Irina Balako
  • Patent number: 10477859
    Abstract: Methods for obtaining genetic material from plant embryos while preserving their viability are provided. In the methods, preservation of viability may be maintained by suspending the embryos in an aqueous solution surrounded by an oil matrix. Genetic material may be obtained from an aliquot of the aqueous solution and may be used directly for molecular analysis, or whole genome amplification may be performed prior to molecular analysis.
    Type: Grant
    Filed: June 4, 2015
    Date of Patent: November 19, 2019
    Inventors: Randal Arnold, Matthew Paul Cope, Justin Andrew Schares, Yue Yun
  • Patent number: 10457905
    Abstract: Aspects of the disclosure relate to rotating bioreactors and articles and methods that are useful for adapting a rotating bioreactor for use with tissues or scaffolds of different sizes. In some embodiments, bioreactors comprising a reservoir and an arbor assembly are provided herein, in which the arbor assembly comprises a rotatable support to which a tissue or tissue scaffold can be attached.
    Type: Grant
    Filed: March 15, 2014
    Date of Patent: October 29, 2019
    Assignee: Biostage, Inc.
    Inventor: Herbert Hedberg
  • Patent number: 10440947
    Abstract: The present invention relates to an aqueous solution for organ preservation, in particular for the myocardium, the solution comprising: 100 mM to 130 mM of Na+; 12 mM to 15 mM of K+; 0.25 mM to 1.3 mM of Ca++; 0.5 ?M to 1.5 ?M of cyclosporin A; and 0.5 ?M to 1.5 ?M of taxol, and it also relates to the joint use of cyclosporin A and taxol in a solution for in vitro preservation of an organ, in particular the myocardium.
    Type: Grant
    Filed: November 13, 2014
    Date of Patent: October 15, 2019
    Inventor: Rene Ferrera
  • Patent number: 10426160
    Abstract: Blood and platelet storage and rejuvenating compositions that include triciribine, triciribine metabolites, triciribine analogs, and mixtures of the same are disclosed. Such compositions can be useful in methods for treating (e.g., storing and rejuvenating) red blood cells and platelets.
    Type: Grant
    Filed: August 24, 2018
    Date of Patent: October 1, 2019
    Assignee: RythRx Therapeutics, LLC
    Inventor: Narayanan Surendran
  • Patent number: 10406259
    Abstract: A method for preparing placenta membrane tissue grafts for medical use, includes obtaining a placenta from a subject, cleaning the placenta, separating the chorion tissue from the amniotic membrane, mounting a selected layer of either the chorion tissue or the amniotic membrane onto a drying fixture, dehydrating the selected layer on the drying fixture, and cutting the selected layer into a plurality of tissue grafts. Preferably, the drying fixture includes grooves or raised edges that define the outer contours of each desired tissue graft, after they are cut, and further includes raised or indented logos that emboss the middle area of the tissue grafts during dehydration and that enables an end user to distinguish the top from the bottom side of the graft. The grafts are comprised of single layers of amnion or chorion, multiple layers of amnion or chorion, or multiple layers of a combination of amnion and chorion.
    Type: Grant
    Filed: July 20, 2017
    Date of Patent: September 10, 2019
    Assignee: MiMedx Group, Inc.
    Inventor: John Daniel
  • Patent number: 10329532
    Abstract: The present invention is directed to a method for producing bioengineered heart muscle (BHM) from pluripotent stem cells, generally comprising the steps of inducing mesoderm differentiation, cardiac differentiation, and cardiac maturation by directed tissue formation. The method is a robust, serum-free and reproducible way to produce BHM for multiple applications, and is applicable to multiple pluripotent stem cell lines. The present invention is also directed to the BHM produced by the method disclosed herein, as well as to uses of said BHM in pharmacologic and toxicity screenings, and its use in medicine.
    Type: Grant
    Filed: September 19, 2014
    Date of Patent: June 25, 2019
    Inventors: Wolfram-Hubertus Zimmermann, James Hudson, Malte Tiburcy
  • Patent number: 10300029
    Abstract: An organ protectant solution which is intravenously administered includes at least one oncotic agent and optionally a high concentration of cell-impermeant molecules. Together, they promote transfer of water from cells to interstitium and into the capillaries, thereby preventing or reducing cell swelling, maintaining blood circulation and oxygenation of tissues, and extending the “Golden Hour” for traumatic and/or hemorrhagic shock patients. In addition, compositions comprising PEG-20k and methods of their use for preserving and/or reanimating harvested organs ex vivo for lengthy periods of time (e.g. at least about 8-24 hours) are also provided.
    Type: Grant
    Filed: October 14, 2016
    Date of Patent: May 28, 2019
    Assignee: Virginia Commonwealth University
    Inventor: Martin Mangino