Including Freezing; Composition Therefor Patents (Class 435/1.3)
  • Patent number: 10894066
    Abstract: Therapeutic compositions are described for the treatment of a variety of conditions including heart, eye, lungs, organs, joints, dermal, nerve, and the like. A therapeutic composition may be a fluid comprising amniotic fluid or micronized amniotic particles. A therapeutic composite may be a dispersion of micronized amniotic membrane combined with a fluid, such as plasma, saline, amniotic fluid, combinations thereof and the like. In another embodiment, the therapeutic composite is a mixture of micronized amniotic membrane particles combined with an amniotic rich stem cell fluid. An amniotic rich or concentrated stem cell fluid comprises at least 0.5×106 amniotic stem cells per milliliter of fluid or composition. A therapeutic composite may be used to treat any number of conditions through topical application, surgical introduction, and/or injection.
    Type: Grant
    Filed: September 6, 2016
    Date of Patent: January 19, 2021
    Assignee: Amnio Technology LLC
    Inventors: Christian Beaudry, Bruce Werber, Terrell Suddarth
  • Patent number: 10827745
    Abstract: The present invention is directed to a vitrification stick for use in the cryopreservation of biological materials, and may include a body having a first portion and a second portion, a specimen end extending from the second portion of the body, and having a cavity defined therein, and a cap including an open end, a closed end, and an aperture, the cap being dimensioned so as to receive at least the specimen end. The cap is positionable relative to the second portion of the body between an open stage and a closed stage, and in the open stage the cavity of the specimen end is exposed by the aperture to an area exterior to the cap.
    Type: Grant
    Filed: January 13, 2020
    Date of Patent: November 10, 2020
    Inventor: Lee L. Nemeth
  • Patent number: 10809269
    Abstract: The present invention relates to the field of human fertility treatment. The present invention more specifically relates to the identification of soluble CD146 (sCD146) as a biomarker which, when measured in an embryo culture medium, can be used to determine whether the embryo can be selected for implantation in the uterus of a mammal or not. The present invention thus provides a new tool and related kits to (pre)select embryo eligible for implantation. The invention also relates to methods for promoting pregnancy in a human who undergoes embryo transfer.
    Type: Grant
    Filed: April 21, 2016
    Date of Patent: October 20, 2020
    Assignees: CENTRE HOSPITALIER UNIVERSITAIRE DE NIMES, INSERM (INSTITUT NATIONAL DE LA SANTE ET DE LA RECHERCHE MEDICALE), UNIVERSITE D'AIX-MARSEILLE, ASSISTANCE PUBLIQUE — HÔPITAUX DE MARSEILLE
    Inventors: Nathalie Bardin, Marcel Blot-Chabaud, Sylvie Bouvier, Odile Lacroix, Francoise Dignat-George, Jean-Christophe Raymond Gris
  • Patent number: 10363278
    Abstract: A frozen therapeutic dose includes an amniotic material and is configured into a pack for easy administering of the dose to a treatment location. A frozen therapeutic dose may contain a concentration of live amniotic stem cells. A frozen therapeutic dose may be provided in a form, such as a multi-pack form, to enable a person to administer a dose to a treatment location without the need of traveling to a doctor's office or clinic. A frozen therapeutic dose package may be kept in a conventional freezer at ?20° C. for example, for extended periods of time and a person may remove the package as needed for treatment. A frozen dose package or pack may contain a secondary material configured to mix with the frozen therapeutic dose. A secondary material may be configured within a single dose compartment with the frozen dose or within a separate compartment.
    Type: Grant
    Filed: December 15, 2016
    Date of Patent: July 30, 2019
    Assignee: Amnio Technology LLC
    Inventors: Christian Beaudry, Terrell Suddarth, Bruce Werber
  • Patent number: 10316314
    Abstract: A nucleic acid extraction method includes the following steps. Firstly, a mixture of a sample and a lysis buffer is provided. The pH of the lysis buffer is lower than 7. Then, the mixture of the sample and the lysis buffer is transferred to a nucleic acid catching chamber containing chitosan coating material, so that nucleic acid of the sample is bound to the chitosan coating material. Then, the chitosan coating material is washed with at least one wash buffer, thereby removing residual protein and cell debris. Then, the chitosan coating material is eluted with an elution buffer while heating the chitosan coating material, so that the nucleic acid is separated from the chitosan coating material. The pH of the elution buffer is higher than 7.
    Type: Grant
    Filed: August 20, 2015
    Date of Patent: June 11, 2019
    Assignee: DELTA ELECTRONICS INT'L (SINGAPORE) PTE LTD
    Inventors: Revata Utama, Yong Zhang, Song Bin Huang, Wang Chu Chen
  • Patent number: 10306882
    Abstract: A biological sample vitrification carrier and a method for freezing a biological sample are provided. Specifically, the carrier comprises a main body and a sealing cap that form a sealed frozen sample placing region. Frozen liquid circulating in a liquid circulating channel in the main body contacts with an outer surface of the frozen sample placing region and cools a biological sample in the frozen sample placing region. The biological sample vitrification carrier and the method in the present invention have the advantages of safety, nontoxicity and high vitrification and rewarming operation efficiency and have wide application prospects in the field of biological sample freezing.
    Type: Grant
    Filed: April 9, 2014
    Date of Patent: June 4, 2019
    Inventors: Wei Lou, Yanping Kuang
  • Patent number: 10188683
    Abstract: The present invention relates to compositions comprising a decellularized tissue. The present invention also provides an engineered three dimensional lung tissue exhibiting characteristics of a natural lung tissue. The engineered tissue is useful for the study of lung developmental biology and pathology as well as drug discovery.
    Type: Grant
    Filed: February 18, 2015
    Date of Patent: January 29, 2019
    Assignee: Yale University
    Inventors: Elizabeth Calle, Laura E. Niklason, Thomas Petersen, Liqiong Gui
  • Patent number: 10188505
    Abstract: Disclosed herein is an improved Bone Tendon Bone graft for use in orthopedic surgical procedures. Specifically exemplified herein is a Bone Tendon Bone graft comprising one or more bone blocks having a groove cut into the surface thereof, wherein said groove is sufficient to accommodate a fixation screw. Also disclosed is a method of harvesting grafts that has improved efficiency and increases the quantity of extracted tissue and minimizes time required by surgeon for implantation.
    Type: Grant
    Filed: March 14, 2017
    Date of Patent: January 29, 2019
    Assignee: RTI Surgical, Inc.
    Inventors: Kevin C. Carter, Michael Esch, Paul LaRochelle, James Gross
  • Patent number: 10178864
    Abstract: A profusion system for a mammalian organ comprising a first and a second pump to propel perfusion fluid through one of a closed fluid circuit system and a selectively closeable fluid circuit system that includes an organ artery and an organ vein, a filter cartridge bank consisting of a plurality of filtration cartridges, and a first and a second fluid reservoir.
    Type: Grant
    Filed: March 7, 2015
    Date of Patent: January 15, 2019
    Assignee: Board of Supervisors of Louisiana State University and Agricultural and Mechanical College
    Inventor: Gary Boucher
  • Patent number: 10174284
    Abstract: [Problem] To provide, in order to manufacture cells to transplant into patients with corneal endothelial failure, a medium used to culture corneal endothelial cells obtained from human corneal tissue and grow said cells while maintaining the morphology thereof as corneal endothelial cells. [Solution] A medium containing a conditioned medium from mesenchymal stem cells; and a method in which said medium is used to culture corneal endothelial cells.
    Type: Grant
    Filed: September 5, 2013
    Date of Patent: January 8, 2019
    Assignees: JCR PHARMACEUTICALS CO., LTD., THE DOSHISHA, KYOTO PREFECTURAL PUBLIC UNIVERSITY CORPORATION
    Inventors: Michio Hagiya, Kiwamu Imagawa, Yuuki Hosoda, Noriko Koizumi, Naoki Okumura, Makiko Nakahara, Shigeru Kinoshita
  • Patent number: 10017735
    Abstract: The invention relates to a dual-media approach for culturing isolated corneal endothelial cells. Isolated corneal endothelial cells are first contacted with a proliferative medium to propagate and/or expand the endothelial cells followed by a maintenance medium to preserve the morphology and/or characteristics of the corneal endothelial cells. The invention includes the proliferative medium and the maintenance medium and also a combination of the two medium.
    Type: Grant
    Filed: July 18, 2013
    Date of Patent: July 10, 2018
    Assignee: SINGAPORE HEALTH SERVICES PTE LTD
    Inventors: Donald Tan, Jodhbir Mehta, Swee Lim Gary Peh
  • Patent number: 9855301
    Abstract: A laminated graft fabricated from human birth tissue material comprising at least two amniotic membranes, or at least two chorionic membranes, or at least one amniotic membrane and at least one chorionic membrane, and at least one morselized placental composition to form a human birth tissue laminate graft. Methods of processing human birth tissue material to form a laminated graft are also provided. Additionally, methods for treating a wound or defect and methods of promoting regeneration of diseased or damaged tissue are provided.
    Type: Grant
    Filed: March 10, 2014
    Date of Patent: January 2, 2018
    Assignee: BioDLogics LLC
    Inventor: Timothy R. Brahm
  • Patent number: 9848598
    Abstract: This invention provides compositions and methods for cryoprotection of recombinant live cancer cells. Specifically, an improved cryoprotective medium is provided which includes a hydroxyethyl starch and/or derivative thereof alone or in combination with either DMSO or glycerol.
    Type: Grant
    Filed: August 12, 2016
    Date of Patent: December 26, 2017
    Assignee: ADURO GVAX INC.
    Inventors: David Frey, Andy Lin, Gautum Banik
  • Patent number: 9700038
    Abstract: The method involves placing an oocyte cell in a cell holder (1), securing the cell holder in a treatment station, applying a treatment solution to the cell by washing the cell with the solution, and rapidly cooling the cell holder and cell to a predetermined cryopreservation temperature for cryopreservation of the cell. The cell is cooled at a high rate sufficient to permit vitrification of the cell and any surrounding treatment solution to occur. The cell is then maintained at or below a predetermined storage temperature for storage. The method allows multiple cells to be treated simultaneously each secured within a respective cell holder.
    Type: Grant
    Filed: September 22, 2014
    Date of Patent: July 11, 2017
    Assignee: Genea Limited
    Inventor: Tomas Stojanov
  • Patent number: 9648869
    Abstract: The invention relates to small molecule ice recrystallization inhibitor (IRI) compounds having increased ice recrystallization inhibition activity. The IRI compounds disclosed herein are useful in compositions, kits and methods for cryopreserving biological material such as organs, tissues and cells.
    Type: Grant
    Filed: May 24, 2013
    Date of Patent: May 16, 2017
    Assignee: THE UNIVERSITY OF OTTAWA
    Inventors: Robert Ben, Chantelle J. Capicciotti
  • Patent number: 9485984
    Abstract: An automated system and method of cryopreservation of oocytes, embryos, or blastocysts. The method entails delivering two or more solutions into a container holding oocytes, embryos, or blastocysts, and controlling the flow of the solutions to gradually change the concentration of cryoprotectants and dehydrating agents in the container to minimize shock to the oocytes, embryos or blastocysts.
    Type: Grant
    Filed: March 18, 2016
    Date of Patent: November 8, 2016
    Assignee: Mariposa Biotechnology, Inc.
    Inventors: Fred H. Burbank, Michael L. Jones
  • Patent number: 9458424
    Abstract: Cryopreserved stem cells with high cell viability after thawing were obtained by slow freezing using a DMSO-free cryopreservation medium. Stem cells and/or progenitor cells thereof are contacted with a DMSO-free cryopreservation medium, comprising between 4 v/v % and 25 v/v % of propylene glycol and between 1.0 w % and 10 w % of a sugar; and are subsequently subject to a slow-freezing process. Optionally, the cryopreservation medium may comprise serum albumin and/or hyaluronic acid.
    Type: Grant
    Filed: December 18, 2014
    Date of Patent: October 4, 2016
    Assignee: FERTIPRO N.V.
    Inventors: Sven Comhaire, Björn Comhaire, Frank Eertmans, Veerle Bogaert
  • Patent number: 9402934
    Abstract: A method for preparing tissue for medical applications, in particular tissue for use for an artificial heart valve, wherein the method has the steps of decellularizing the tissue by means of a detergent and subsequently cross-linking the collagen fibers of the tissue by means of a suitable cross-linking agent. At least one lipopeptide, such as surfactin, for example, is used as the detergent for decellularization.
    Type: Grant
    Filed: July 26, 2014
    Date of Patent: August 2, 2016
    Assignee: BIOTRONIK AG
    Inventors: Alexander Rzany, Wilhelm Erdbruegger
  • Patent number: 9376661
    Abstract: The invention provides a method of culturing a corneal endothelial cell by use of a culture medium containing an ascorbic acid derivative and the like.
    Type: Grant
    Filed: February 4, 2011
    Date of Patent: June 28, 2016
    Assignee: Cornea Regeneration Institute Co., Ltd.
    Inventors: Nobuyuki Shima, Miwa Yamauchi, Masahiro Yamaguchi, Satoru Yamagami
  • Patent number: 9314015
    Abstract: A method and apparatus are disclosed for avoiding fracturing, e.g., thermo-mechanical fracturing, in vitrified biological systems via rapid cooling and/or warming persufflation techniques, by reducing the domain size of fracturing and by reducing thermal gradients. Also disclosed is a system adapted to rapidly cool and warm vitrifiable vascular biological tissue by persufflation, significantly reducing cryoprotectant toxicity from that of surface cooled tissue, in which the system is constructed and configured to use one or more of helium gas, hydrogen gas, neon gas, argon gas, krypton gas, xenon gas, oxygen gas, or various gaseous compounds. The system can be operated under pressure to increase the density and heat capacity of the gas relative to its density and heat capacity at atmospheric pressure and to cool the gas by one or more of mechanical action and by the phase change of a material such as a cryogenic gas or solid.
    Type: Grant
    Filed: July 5, 2013
    Date of Patent: April 19, 2016
    Assignee: Arigos Biomedical, Inc.
    Inventors: Stephen Van Sickle, Tanya Jones
  • Patent number: 9055739
    Abstract: A composition for the cryopreservation of cells according to the present invention at least comprises sericin and one or more components selected from the group consisting of amino acids and saccharides. Further, a method for the cryopreservation of cells according to the present invention comprises the steps of placing target cells in the abovementioned composition for the cryopreservation of cells and cryopreserving them. According to the composition for the cryopreservation of cells of the present invention, cells can be cryopreserved over a long period of time without the use of serum and components derived from serum.
    Type: Grant
    Filed: September 26, 2005
    Date of Patent: June 16, 2015
    Assignee: SEIREN KABUSHIKI KAISHA
    Inventors: Yoichi Kato, Masahiro Sasaki, Hideyuki Yamada
  • Publication number: 20150104434
    Abstract: The present invention provides an artificial kidney precursor containing a non-human mammalian metanephros separated out from a living body, wherein the metanephros has been subjected to freezing and thawing treatments outside a living body, and contains mammalian mesenchymal stem cells transferred outside a living body, and a method of production thereof.
    Type: Application
    Filed: December 9, 2014
    Publication date: April 16, 2015
    Inventors: Eiji KOBAYASHI, Takashi YOKOO, Koutaro KAI
  • Patent number: 8980630
    Abstract: The present invention relates to stem cells obtained from the amnion and their methods of obtaining and culturing. The present invention further relates to compositions comprising amnion-derived stems cells (ADSCs) and to methods of using ADSCs.
    Type: Grant
    Filed: June 28, 2007
    Date of Patent: March 17, 2015
    Assignee: Rutgers, The State University of New Jersey
    Inventors: Dale Woodbury, Akiva J. Marcus
  • Patent number: 8956411
    Abstract: The present invention relates to a method of preparing a tissue matrix and its subsequent use in the replacement and/or repair of a damaged or defective meniscus. The invention also provides meniscal tissue that is substantially decellularised.
    Type: Grant
    Filed: November 13, 2007
    Date of Patent: February 17, 2015
    Assignee: University of Leeds
    Inventors: Eileen Ingham, John Fisher, Thomas Stapleton, Joanne Ingram
  • Publication number: 20150037783
    Abstract: An improved cryopreservation process and substances can involve a cellular collection (1) in a cryopreservation fluid (4) that has been conditioned or treated (7) to enhance the cryopreservation process by adding (18) energy (19) such as in the surface energy of a substance in the cryopreservation fluid (4) prior to reducing energy for that same cryopreservation media for freezing. This can offer enhanced-post-cryogenic viability of the cryopreserved structures or a more optimum cooling curve (22) for a specific cell type.
    Type: Application
    Filed: March 11, 2013
    Publication date: February 5, 2015
    Inventors: Lisa A. Herickhoff, Patrick D. Burns, Nicole R. White, James A. Herickhoff
  • Publication number: 20150037782
    Abstract: Novel methods and systems for electromagnetic freezing are disclosed. An oscillating magnetic field can be applied in conjunction with a static magnetic field in order to align magnetic particles inside biological tissues. Such chains may be naturally present or artificially introduced. Needle-shaped electrodes may be used to produce ions and disturb the air layers insulating the tissues to be frozen.
    Type: Application
    Filed: July 10, 2014
    Publication date: February 5, 2015
    Inventors: Atsuko KOBAYASHI, Joseph L. KIRSCHVINK
  • Patent number: 8936651
    Abstract: Methods for defatting omentum and processes for preparing an acellular omentum, i.e., devitalized or decellularized omentum, comprising extracellular matrix for implantation into a mammalian system. Constructs for medical applications comprising decellularized omentum are also described. More specifically, mesh reinforced omentum biomatrix for soft tissue repair is described.
    Type: Grant
    Filed: March 14, 2013
    Date of Patent: January 20, 2015
    Assignee: Ethicon, Inc.
    Inventors: Chunlin Yang, Qiang Zhang, Ilya Koyfman, Ziwei Wang, Daphne Ann Salick Ryan, Robert B. Vetrecin, Philip M. Steele
  • Publication number: 20150017628
    Abstract: The present invention relates to a method for cryopreserving biological material. In particular, the method comprises storing the biological material at a cryopreserving temperature in a composition comprising polyvinyl alcohol (PVA). wherein the composition is substantially free of vitrification-inducing agents such as DMSO and glycerol. The invention also provides methods of inhibiting ice recrystallisation and of reducing cell damage during the warming or thawing of a cryopreserved composition comprising biological material. The invention also relates to processes for producing a biological material, and related kits.
    Type: Application
    Filed: February 7, 2013
    Publication date: January 15, 2015
    Applicant: UNIVERSITY OF WARWICK
    Inventors: Matthew I. Gibson, Robert C. Deller
  • Patent number: 8932805
    Abstract: Methods of preparing a human birth tissue material are provided. A placental construct for treatment of a disease or condition is also provided. A kit including at least one placental construct and at least one structural carrier is provided. A catheter for recovering amniotic fluid is also provided.
    Type: Grant
    Filed: October 31, 2012
    Date of Patent: January 13, 2015
    Assignee: BioDlogics, LLC
    Inventor: Timothy R. Brahm
  • Patent number: 8927459
    Abstract: A method is provided for inhibiting plant growth by inducing hypothermia by applying a treating substance to summer annual weeds and grasses. Susceptible undesirable plants will be killed by this treatment, while non-susceptible desirable plants will not be adversely affected by this treatment. Compressed carbon dioxide may be applied to other summer grasses to cause the grasses to become dormant. An apparatus is provided for inducing hypothermia in undesirable plants by applying a treating substance. A canister capable of housing gas may be provided. A valve may be provided to control the amount and pressure of gas dispensed from the canister.
    Type: Grant
    Filed: March 8, 2013
    Date of Patent: January 6, 2015
    Assignee: Arctic, Inc.
    Inventors: Robby James Howerton, Gina Marie Stewart, Steven Lee Worm
  • Patent number: 8916339
    Abstract: A composition of neural tissue is made of spinal cord tissue harvested from a spinal cord of a mammal. The spinal cord tissue is harvested from vertebral column of the mammal. The spinal cord tissue is harvested post mortem. The mammal is one of a primate, an equine, a bovine, a porcine or other mammalian animal. The spinal cord tissue is dried or has the water content in the tissue reduced or eliminated by freeze drying or hypothermic dehydration.
    Type: Grant
    Filed: March 7, 2014
    Date of Patent: December 23, 2014
    Assignee: Vivex Biomedical, Inc.
    Inventor: Theodore I. Malinin
  • Publication number: 20140356850
    Abstract: Systems and methods of the invention generally relate to prolonging viability of bodily tissue in static cold storage and hypothermic machine perfusion techniques by reducing the rate of edema through the application of compressive force to the bodily tissue via a compression device. Compression devices of the invention may comprise biocompatible materials and may apply compressive force through incorporation of elastic materials, through expandable bladders, or other means. Certain embodiments include perfusion devices comprising a pneumatic system, a pumping chamber, and an organ chamber. The pumping chamber is configured to diffuse a gas into a perfusate and to generate a pulse wave for moving the perfusate through a bodily tissue. The organ chamber is configured to receive the perfusate along with a bodily tissue fitted with a compression device. Both the organ chamber and the pumping chamber are configured to substantially automatically purge excess fluid.
    Type: Application
    Filed: August 15, 2014
    Publication date: December 4, 2014
    Inventors: Lisa Maria Anderson, Jared Alden Judson
  • Publication number: 20140342346
    Abstract: The invention provides a method of cryopreserving a tissue derived from a pluripotent stem cell, including the steps of (1) bringing a tissue derived from a pluripotent stem cell into contact with a cell protection solution that contains sulfoxide and chain polyol, (2) maintaining, in a cryopreservation solution, the pluripotent stem cell-derived tissue that was brought into contact with the cell protection solution in the first step, and (3) cryopreserving, in the presence of a coolant, the pluripotent stem cell-derived tissue that was maintained in the cryopreservation solution.
    Type: Application
    Filed: November 22, 2012
    Publication date: November 20, 2014
    Inventors: Satoshi Ando, Tokushige Nakano, Yoshiki Sasai, Mototsugu Eiraku
  • Patent number: 8883210
    Abstract: The disclosure provides implants containing a plurality of particles containing at least one population of viable tissuegenic cells adherent to and resident in the growth-conductive matrix or at least viable population of tissuegenic cells caused to be in contact with the growth-conductive matrix; methods to fabricate implants; methods of fabricating the implants; and use of the implants in tissue repair.
    Type: Grant
    Filed: May 16, 2011
    Date of Patent: November 11, 2014
    Assignee: Musculoskeletal Transplant Foundation
    Inventors: Katherine Gomes Truncale, Arthur A. Gertzman, Yen-Chen Huang, Eric Semler, Evangelia Chnari, Anouska Dasgupta, Judith Yannariello-Brown, Terri Riley
  • Patent number: 8883408
    Abstract: A method is provided for preparing a tissue implant for implantation. The method includes harvesting a tissue material from a human or an animal donor, treating the tissue material in a nuclease-containing solution, and thereafter treating the tissue material with an alkaline alcohol solution. The nuclease-containing solution includes an antimicrobial. The alkaline alcohol solution comprises sodium hydroxide and ethanol.
    Type: Grant
    Filed: January 30, 2013
    Date of Patent: November 11, 2014
    Assignee: CryoLife, Inc.
    Inventors: Joseph Hamby, Steven Walsh
  • Publication number: 20140301986
    Abstract: Provided herein is a placental product comprising an immunocompatible chorionic membrane. Such placental products can be cryopreserved and contain viable therapeutic cells after thawing. The placental product of the present invention is useful in treating a patient with a tissue injury (e.g. wound or burn) by applying the placental product to the injury. Similar application is useful with ligament and tendon repair and for engraftment procedures such as bone engraftment.
    Type: Application
    Filed: May 30, 2014
    Publication date: October 9, 2014
    Applicant: Osiris Therapeutics, Inc.
    Inventors: Samson Tom, Alla Danilkovitch, Dana Yoo, Timothy Jansen, Jin-Qiang Kuang, Jennifer Michelle Marconi
  • Publication number: 20140271583
    Abstract: The present invention relates generally to systems and methods for preparing, storing, shipping and using skin equivalents made by organotypic culture. In particular, the present invention relates to systems and methods for cryopreserving viable skin substitutes.
    Type: Application
    Filed: March 13, 2014
    Publication date: September 18, 2014
    Inventors: B. Lynn Allen-Hoffmann, John C. Pirnstill, Kenneth R. Gratz, Allen R. Comer
  • Patent number: 8828448
    Abstract: The invention provides an improved method of producing a natural, acellular matrix scaffold for subsequent use in tissue-engineered replacement of tissues such as the bladder. Decellularization is carried out on an expanded or distended bladder and the product retains the strength and compliance of natural material. The invention also provides use of the matrix scaffolds as wound healing material and to investigate tissue structure and function in vitro.
    Type: Grant
    Filed: March 28, 2007
    Date of Patent: September 9, 2014
    Assignee: Tissue Regenix Limited
    Inventors: Sotirios Korossis, Eileen Ingham, Fiona Bolland, Jennifer Southgate
  • Publication number: 20140243426
    Abstract: A composition for cryopreservation of cells and tissues of human and other animals in a safe manner without using toxic substances such as DMSO, as well as for freeze preserving or freeze-drying of foods and pharmaceuticals. In embodiments, ?-poly-L-lysine is reacted with succinic anhydride so that 60% or more of amino groups are blocked; and, thus obtained polymer compound is added to Dulbecco-modified eagle MEM culture medium (DMEM) on market sale to form a cryopreservation liquid. In embodiments for foods or pharmaceuticals, the ?-poly-L-lysine derivative was added by 0.5-10 wt % to curb freeze concentration.
    Type: Application
    Filed: April 30, 2014
    Publication date: August 28, 2014
    Applicant: Bio Verde Inc.
    Inventors: Kazuaki MATSUMURA, Hajime SUGAI, Suong-Hyu HYON
  • Publication number: 20140234427
    Abstract: The invention provides a method of preventing or reducing the growth of crystals in a substance which is susceptible to crystal growth in which colloidal particles having an amphiphilic structure, e.g. Janus particles, are contacted with the substance. Colloidal particles suitable for use in the invention include cross-linked, colloidal materials formed from hydrophobic monomers such as acrylates or methacrylates and hydrophilic monomers such as those derived from acrylic and/or methacrylic acid. The colloidal particles find particular use in methods of cryopreservation of biological samples (e.g. cells, tissues or organs), as a texture modifier in frozen food products, in the inhibition of gas hydrate formation, and as scale inhibitors.
    Type: Application
    Filed: October 5, 2012
    Publication date: August 21, 2014
    Applicant: UNIVERSITY OF WARWICK
    Inventors: Matthew Ian Gibson, Stefan Antonius Franciscus Bon
  • Publication number: 20140220551
    Abstract: Disclosed herein are methods for cryopreserving cells and tissues under clinical conditions, allowing production of viable cell products suitable for transplantation.
    Type: Application
    Filed: February 3, 2014
    Publication date: August 7, 2014
    Applicant: PrimeGen Biotech LLC
    Inventors: Fariborz Izadyar, Jason Pacchiarotti, Marnie Olmstead, Kyle Howerton, Thomas Ramos
  • Publication number: 20140127317
    Abstract: Provided herein is a placental product comprising an immunocompatible amniotic membrane. Such placental products can be cryopreserved and contain viable therapeutic cells after thawing. The placental product of the present invention is useful in treating a patient with a tissue injury (e.g. wound or burn) by applying the placental product to the injury. Similar application is useful with ligament and tendon repair and for engraftment procedures such as bone engraftment.
    Type: Application
    Filed: November 1, 2013
    Publication date: May 8, 2014
    Applicant: Osiris Therapeutics, Inc.
    Inventors: Timothy Jansen, Samson Tom, Alla Danilkovitch, Dana Yoo, Jaime Zerhusen
  • Publication number: 20140127172
    Abstract: A method of preserving functionality of an organ is disclosed herein, including removing a whole organ including vasculature from a donor, cryo-preserving the whole organ including vasculature, thawing the whole organ including vasculature so that at least a portion of cells contained therein resume biological activity, and introducing the whole organ including vasculature into a recipient so that a blood vessel in the vasculature is supplied with blood. Further disclosed herein is a method of preserving fertility of a patient undergoing a treatment expected to cause sterility, including removing at least one whole gonadal organ including vasculature from the patient, preserving functionality of the gonadal organ as described herein, conducting the treatment and waiting for an effect thereof to subside, and introducing the gonadal organ into the patient.
    Type: Application
    Filed: November 8, 2013
    Publication date: May 8, 2014
    Inventors: Amir ARAV, Yehudit NATAN, Saar YAVIN, Uri MEIR, Udi DAMARI
  • Publication number: 20140120068
    Abstract: This disclosure provides solutions, systems, and methods for cell, tissue, and/or organ preservation. Some preservation solutions may include any combination of a balanced salt solution, electrolytes, antibiotic agents, antimycotic agents, protease inhibitors, anti-oxidants, simple sugars, starches impermeant ions, uric acid and/or amino acids. Some preservation solutions may also include hydrolyzed collagen. The preservation solutions including hydrolyzed collagen may be used alone or as part of a kit to preserve cells, tissue, or organs. The solution may also be used in connection with one or more medical procedures, for example organ transplantation.
    Type: Application
    Filed: November 1, 2013
    Publication date: May 1, 2014
    Applicant: Animal Cell Therapies, Inc.
    Inventors: Kathy Petrucci, Chih-Min Lin
  • Patent number: 8703411
    Abstract: A method of preserving an umbilical cord is disclosed. The method comprises obtaining a segment of an umbilical cord; mincing the segment of the umbilical cord into cord tissue pieces; admixing the cord tissue pieces with a cryogenic composition comprising a cryoprotectant and a protein to form a mixture; shaking the mixture for a duration of no shorter than 20 minutes and no longer than 40 minutes; and cryopreserving the mixture.
    Type: Grant
    Filed: September 26, 2011
    Date of Patent: April 22, 2014
    Assignee: HealthBanks Biotech Co., Ltd.
    Inventors: Hsiu-Kang Chang, Wei-Yu Lo
  • Patent number: 8679735
    Abstract: Methods and compositions are provided for the cryopreservation of human organs and tissues. In certain embodiments, Step 1 comprises perfusion with a vitrifiable cryoprotectant solution at a temperature above ?10° C. for a time insufficient for the approximate osmotic equilibration of the organ with the solution, followed by cooling the organ to below ?10° C. by perfusion with said solution at a reduced temperature. In certain embodiments, Step 2 comprises increasing the concentration of cryoprotectant further at a temperature from ?10 to ?40° C. In certain embodiments, Step 3 comprises cooling and vitrifying the organ, rewarming it, and perfusing the organ with a vitrifiable concentration of cryoprotectant whose temperature is either raised gradually or is held at ??15° C. Compositions are provided that allow safe organ perfusion with vitrifiable media at >?10° C. and almost complete avoidance of chilling injury at ?20 to ?25° C. and that allow slow warming after vitrification without freezing.
    Type: Grant
    Filed: September 16, 2004
    Date of Patent: March 25, 2014
    Assignee: 21st Century Medicine Inc.
    Inventors: Gregory M. Fahy, Brian Wowk
  • Publication number: 20140030231
    Abstract: The present invention generally relates to methods and compositions to determine viability of an organ for transplantation and other medical purposes. One aspect of the invention relates to a method for assessing the viability of an organ by measuring the energy parameters to determine the energy level of the organ by determining the stored cellular energy (e.g., ATP levels), and/or energy consumption over a particular time period of viability. The energy parameters can be compared to reference energy parameters as a highly accurate and reliable prediction of viable cell yield, and organ viability. Another aspect of the invention relates methods to preserve or extend the time period of viability of an organ any combination of (i) preservation perfusion of the organ to prevent ischemic damage, (ii) chemical metabolic suppression of the organ e.g., using metabolic suppressants, (iii) metabolic suppression by physical or environmental conditions, e.g., sub-zero non-freezing storage.
    Type: Application
    Filed: May 4, 2011
    Publication date: January 30, 2014
    Applicant: THE GENERAL HOSPITAL CORPORATION
    Inventors: Martin L. Yarmush, Mehmet Toner, Maria-Louisa Izamis, Timothy Antonie Berendsen, Robert Marius Bieganski, Osman Berk Usta, Basak Elif Uygun, Mustafa Korkut Uygun, Sinem Perk
  • Publication number: 20140024011
    Abstract: This disclosure presents methodologies for processing, cryogenic storage, retrieval and preparation of a tissue for transplantation. As such, the methods provide a system by which tissue for transplantation can be reliably and safely processed and stored for an extended period of time until the tissue is desired to be used.
    Type: Application
    Filed: July 23, 2013
    Publication date: January 23, 2014
    Inventor: David Thomas Harris
  • Publication number: 20140011182
    Abstract: A method and apparatus are disclosed for avoiding fracturing, e.g., thermo-mechanical fracturing, in vitrified biological systems via rapid cooling and/or warming persufflation techniques, by reducing the domain size of fracturing and by reducing thermal gradients. Also disclosed is a system adapted to rapidly cool and warm vitrifiable vascular biological tissue by persufflation, significantly reducing cryoprotectant toxicity from that of surface cooled tissue, in which the system is constructed and configured to use one or more of helium gas, hydrogen gas, neon gas, argon gas, krypton gas, xenon gas, oxygen gas, or various gaseous compounds. The system can be operated under pressure to increase the density and heat capacity of the gas relative to its density and heat capacity at atmospheric pressure and to cool the gas by one or more of mechanical action and by the phase change of a material such as a cryogenic gas or solid.
    Type: Application
    Filed: July 5, 2013
    Publication date: January 9, 2014
    Inventors: Stephen Van Sickle, Tanya Jones
  • Patent number: 8623591
    Abstract: Methods of isolating cellular products, such as pancreatic islets, may be used in diabetes research and therapeutic transplantation. The methods may involve providing a tissue having desired cells that are less prone to destructive freezing and undesired cells that are more prone to destructive freezing, or pre-treating a tissue to have such characteristics. The methods may involve freezing the tissue, disrupting the tissue, warming the tissue, and separating the desired cells from undesired cellular material to obtain the cellular product. The methods may thereby provide an enzyme-free or reduced-enzyme method of isolating a cellular product that is more consistent, reliable and less toxic than conventional methods. The methods may also yield an optimum quantity of cellular product that retain sufficient functional integrity to be useful as a transplantation resource.
    Type: Grant
    Filed: August 27, 2012
    Date of Patent: January 7, 2014
    Assignee: Lifeline Scientific, Inc.
    Inventors: Michael J. Taylor, David E. Pegg