Involving Urea Or Urease Patents (Class 435/12)
  • Patent number: 10562024
    Abstract: The present disclosure relates to paper-based substrates and apparatus comprising such substrates. The apparatus may include a patterned conductive structure coupled to the paper-based substrate, wherein the patterned conductive structure responds to electromagnetic radiation.
    Type: Grant
    Filed: January 4, 2012
    Date of Patent: February 18, 2020
    Assignee: Tufts University
    Inventors: Fiorenzo Omenetto, David L. Kaplan, Hu Tao
  • Patent number: 10509025
    Abstract: The invention provides a poor physical condition determination device, method, and program enabling simple determination of a physical condition level. A poor physical condition determination device 10 acquires a ketone concentration measurement measuring ketone excreted from a user, determines a physical condition level related to the physical condition of the user based on the acquired ketone concentration measurement, and outputs a determination result.
    Type: Grant
    Filed: January 22, 2015
    Date of Patent: December 17, 2019
    Assignee: Tanita Corporation
    Inventors: Miyuki Kodama, Ayumi Sano, Naotaka Minagawa, Yasuhiro Kasahara
  • Patent number: 10471088
    Abstract: This invention relates to solid oral compositions based on SAMe and/or NADH or their salts in association with calcium oxide and/or calcium hydroxide and a process for obtaining them. This invention also relates to a method for stabilising a solid oral composition based on SAMe and/or NADH or their salts, making use of calcium oxide, calcium hydroxide optionally in association with malic acid, glutamic acid, xylitol, calcium sulphate hemihydrate, magnesium oxide and/or mixtures thereof. This invention also relates to the use of SAMe or its salts in association with calcium oxide and/or calcium hydroxide with the possible further addition of melatonin and/or 1-theanine and/or 1-tryptophan and/or 5-hydroxytryptophan for the treatment of depressive states.
    Type: Grant
    Filed: May 21, 2015
    Date of Patent: November 12, 2019
    Assignee: GNOSIS SPA
    Inventors: Daniele Giovannone, Carlo De Angelis
  • Patent number: 10260085
    Abstract: In one form, a diagnostic element for determining at least one analyte is provided. In a further form, an analytical measuring device includes the diagnostic element. Still, other forms are related to methods for the determination of an analyte, correcting a signal generated by an analyte, and/or checking the detection optics of an analytical measuring device using the diagnostic element. Another form is related to a system for the controlled release of a reagent and the use of such a system as a circuit element. Other aspects include, but are not limited to, unique methods, techniques, products, systems and devices involving diagnostic elements.
    Type: Grant
    Filed: June 15, 2012
    Date of Patent: April 16, 2019
    Assignee: Roche Diabetes Care, Inc.
    Inventors: Carina Horn, Dieter Heindl, Hans-Peter Haar, Nelli Steinke
  • Patent number: 9879214
    Abstract: A thin film culture device for detecting yeast and mold microorganisms in a sample is provided. The culture device comprises a body comprising a self-supporting substrate having a first major surface and a second major surface; a first adhesive composition disposed on a portion of the first major surface of the substrate; a substantially dry, cold-water-soluble first hydrogel-forming composition adhered to the first adhesive composition; and a plurality of indicator agents. The plurality of indicator agents comprises three indicator agents for detecting distinct glycosidase enzyme activities, an indicator agent for detecting an alkyl esterase enzyme activity, and an indicator agent for detecting a phosphatase enzyme activity, wherein each of the plurality of indicator agents comprises a detectable reporter group. A method of using the culture device is also provided.
    Type: Grant
    Filed: July 30, 2015
    Date of Patent: January 30, 2018
    Assignee: 3M Innovative Properties Company
    Inventors: Sailaja Chandrapati, Tera M. Nordby
  • Patent number: 9863958
    Abstract: The present invention provides a method for diagnosing venous thromboembolism (VTE), comprising: detecting the level of an integrin ?1 subunit, an integrin ?2 subunit, and/or an integrin ?3 subunit in a blood sample. Also provided is a reagent kit for diagnosing VTE, comprising a substance capable of specifically binding to the integrin ?1 subunit, the integrin ?2 subunit, and/or the integrin ?3 subunit.
    Type: Grant
    Filed: April 18, 2012
    Date of Patent: January 9, 2018
    Inventor: Lemin Wang
  • Patent number: 9861245
    Abstract: A nonwoven wipe having a reversible sanitizer indicator for multiple uses and recharges is provided. The nonwoven wipe includes a cloth-like nonwoven fabric coated with a reversible color-changing ink formulation. During use, the nonwoven wipe is impregnated with a quaternary ammonium compound-based sanitizer. When the level of free quaternary ammonium compound falls below a threshold level, the color-changing ink formulation changes from a first color to a second color, indicating the need to recharge the wipe. When the nonwoven wipe is recharged with sanitizer solution, the color-changing ink formulation changes back to the first color.
    Type: Grant
    Filed: May 22, 2014
    Date of Patent: January 9, 2018
    Assignee: Illinois Tool Works, Inc.
    Inventors: Darren J. Farrugia, Lesley J. Ward, Veronica R. Reichert
  • Patent number: 9784730
    Abstract: Nanoparticle having a crosslinked chitosan-polyethylene oxide oligomer copolymer coating to which O6-benzylguanine is covalently coupled, compositions that include the nanoparticle, and methods for using the nanoparticle to treat brain cancers.
    Type: Grant
    Filed: March 21, 2014
    Date of Patent: October 10, 2017
    Assignee: University of Washington through its Center for Commercialization
    Inventors: Miqin Zhang, Richard G. Ellenbogen, Forrest Kievit, John R. Silber, Zachary Stephen, Omid Veiseh
  • Patent number: 9458500
    Abstract: The present invention relates to a novel method for analyzing nucleic acid sequences based on real-time detection of DNA polymerase-catalyzed incorporation of each of the four nucleotide bases, supplied individually and serially in a microfluidic system, to a reaction cell containing a template system comprising a DNA fragment of unknown sequence and an oligonucleotide primer. Incorporation of a nucleotide base into the template system can be detected by any of a variety of methods including but not limited to fluorescence and chemiluminescence detection. Alternatively, microcalorimetic detection of the heat generated by the incorporation of a nucleotide into the extending template system using thermopile, thermistor and refractive index measurements can be used to detect extension reactions.
    Type: Grant
    Filed: June 8, 2013
    Date of Patent: October 4, 2016
    Assignee: Life Technologies Corporation
    Inventors: Peter Williams, Thomas J. Taylor, Daniel J. B. Williams, Ian Gould, Mark A. Hayes
  • Patent number: 9410179
    Abstract: The present invention relates to a diagnostic method for the determination of Helicobacter pylori in a tissue biopsy comprising the step of incubating a tissue biopsy with an aqueous diagnostic reagent comprising urea and a pH indicator, and determining any pH change, characterized in that said diagnostic reagent comprises a lactone, and that the ratio between the volume of the reagent and the volume of the sample is variable and less than 30.
    Type: Grant
    Filed: March 7, 2008
    Date of Patent: August 9, 2016
    Assignee: ABS ADVANCED BIOMEDICAL SYSTEMS SRL
    Inventor: Paolo Giordano
  • Patent number: 9366679
    Abstract: A method for the diagnosis and/or therapy control of systemic sclerosis is disclosed wherein soluble CD90 is detected in body fluids. By measuring the concentration of soluble CD90 certain forms of diseases can be differentiated by applying the ex vivo method.
    Type: Grant
    Filed: March 28, 2013
    Date of Patent: June 14, 2016
    Assignee: Universitaetsklinikum Freiburg
    Inventors: Florian Kollert, Anja Saalbach
  • Patent number: 9347941
    Abstract: A method for diagnosing rheumatoid arthritis, based on the detection of citrullinated autoantigens in a test sample is disclosed. The method diagnoses rheumatoid arthritis more accurately and rapidly. Also disclosed is a kit for carrying out the method.
    Type: Grant
    Filed: October 31, 2012
    Date of Patent: May 24, 2016
    Assignee: CATHOLIC UNIVERSITY INDUSTRY-ACADEMIC COOPERATION FOUNDATION
    Inventors: Ji-Hyeon Ju, Young-Kyun Kim
  • Patent number: 9212393
    Abstract: The present invention relates to a novel method for analyzing nucleic acid sequences based on real-time detection of DNA polymerase-catalyzed incorporation of each of the four nucleotide bases, supplied individually and serially in a microfluidic system, to a reaction cell containing a template system comprising a DNA fragment of unknown sequence and an oligonucleotide primer. Incorporation of a nucleotide base into the template system can be detected by any of a variety of methods including but not limited to fluorescence and chemiluminescence detection. Alternatively, microcalorimetic detection of the heat generated by the incorporation of a nucleotide into the extending template system using thermopile, thermistor and refractive index measurements can be used to detect extension reactions.
    Type: Grant
    Filed: May 3, 2011
    Date of Patent: December 15, 2015
    Assignee: Life Technologies Corporation
    Inventor: Peter Williams
  • Patent number: 9176124
    Abstract: The determination method of the present invention includes steps of measuring equol in a biological sample derived from a subject who has ingested soybean isoflavone by an immunological method using S-equol as at least one antigen selected from the group consisting of the standard antigen and the labeled antigen, and determining an equol-producing ability of the subject based on the measured value of equol obtained in the above step.
    Type: Grant
    Filed: May 11, 2010
    Date of Patent: November 3, 2015
    Assignees: OTSUKA PHARMACEUTICAL CO., LTD., EIKEN KAGAKU KABUSHIKI KAISHA
    Inventors: Takayuki Minekawa, Kumiko Shindome, Katsushi Abe, Hiroshi Okuma, Chieko Ando, Yasuhiro Abiru
  • Patent number: 9151765
    Abstract: Hemoglobin in a sample solution is quickly and reliably denatured; at the same time, quick and accurate measurement of hemoglobin and a hemoglobin derivative is realized. In a method for measuring hemoglobin and a hemoglobin derivative, and a reagent composition, a measurement kit, an analysis device, and an analysis system used in the method, a sample solution containing a blood component is treated with a nonionic surfactant, an oxidizing agent, and a metal salt to denature hemoglobin in the sample solution to measure the hemoglobin, and thereafter the amount of a hemoglobin derivative in the sample is measured by an immunological method using an antibody specifically binding to a denatured site of the denatured hemoglobin derivative.
    Type: Grant
    Filed: September 12, 2013
    Date of Patent: October 6, 2015
    Assignee: Panasonic Healthcare Holdings Co., Ltd.
    Inventors: Hirotaka Tanaka, Masanori Tanaka, Chizu Asahara, Fumihisa Kitawaki
  • Patent number: 9146237
    Abstract: The present application relates to a novel method of in vitro assay of the molecules of the extracellular matrix synthesized by cells in culture and uses thereof in the form of a kit for in vitro measurement and/or for a method of screening of cosmetic and/or pharmaceutical ingredients.
    Type: Grant
    Filed: June 18, 2012
    Date of Patent: September 29, 2015
    Assignee: BASF Beauty Care Solutions France S.A.S.
    Inventors: Sébastien Bonnet, Delphine Rival
  • Patent number: 9097716
    Abstract: The present invention relates to a method for determining the presence of anti-Tr antibodies in a subject comprising the steps of obtaining a sample from said subject testing the presence of said antibodies in said sample by addition of DNER protein or an antigenic part thereof and checking whether said DNER protein is bound by any antibodies in said sample Such an assay is useful for the diagnosis of paraneoplastic cerebellar degeneration that is associated with Hodgkin lymphoma, or, more generally, to type patients suffering from cerebellar ataxia. Also comprised in the invention is a kit for performing such an assay.
    Type: Grant
    Filed: March 1, 2012
    Date of Patent: August 4, 2015
    Assignee: Erasmus University Medical Center
    Inventors: Petrus Abraham Elisa Sillevis Smitt, Esther De Graaff
  • Patent number: 9081013
    Abstract: It is intended to provide a method for detecting gastric cancer, which is low invasive to a human test subject and has high detection sensitivity and accuracy. The present invention provides a method comprising measuring in vitro the amount of COTL1 protein, a variant thereof, and/or a fragment thereof in a body fluid sample derived from a human test subject, and detecting the presence or absence of gastric cancer affecting the test subject on the basis of the amount, and a kit for gastric cancer diagnosis comprising an antibody capable of specifically binding to the protein.
    Type: Grant
    Filed: March 3, 2011
    Date of Patent: July 14, 2015
    Assignees: TORAY INDUSTRIES, INC., KYOTO UNIVERSITY
    Inventors: Yoshinori Tanaka, Satoko Kanamori, Michimoto Kobayashi, Giman Jung, Yoshiharu Sakai, Hiroshi Okabe
  • Patent number: 9063148
    Abstract: Methods and kits are provided for assessing radiation injury and exposure in a mammal. The methods comprise the steps of: obtaining one or more test samples from the mammal, contacting the test samples with an antibody immunoreactive with a citrullinated protein to form an immunocomplex; and detecting the immunocomplex with an ELISA; wherein a decrease in the quantity of the immunocomplex in the test samples, as compared to the quantity of immunocomplexes formed under identical conditions with the same antibody and a control sample from one or more mammals known to have a lower degree of radiation injury or exposure, indicates a higher degree of radiation injury and exposure to the mammal. The information obtained from such methods can be used by a clinician to accurately assess the extent of radiation injury/exposure in the mammal, and thus will provide a valuable tool for determining treatment protocols on a subject by subject basis.
    Type: Grant
    Filed: October 22, 2009
    Date of Patent: June 23, 2015
    Assignee: THE HENRY M. JACKSON FOUNDATION FOR THE ADVANCEMENT OF MILITARY MEDICINCE, INC.
    Inventor: Natalia Ossetrova
  • Patent number: 9046532
    Abstract: A diagnostic method for the identification of a subject suffering from a primary non-infectious disease having an increased risk of an adverse outcome potentially being induced by the administration of an antibiotic to said subject comprising the determination of the level of Procalcitonin (PCT) or a fragment thereof or a precursor or fragment thereof having a length of at least 12 amino acid residues in a sample of a bodily fluid from said subject and the correlation of the determined level to a potential risk induced by the administration of an antibiotic, as well as a kit for performing the method and methods of treatment based thereon.
    Type: Grant
    Filed: April 14, 2010
    Date of Patent: June 2, 2015
    Assignee: B.R.A.H.M.S GMBH
    Inventors: Andreas Bergmann, Joachim Struck
  • Publication number: 20150064732
    Abstract: An analytical test cartridge is provided. The analytical test cartridge can be used for medical analyses of liquid samples removed from a patient, for example blood. The analytical test cartridge is configured to provide for titration experiments. An example of a titration experiment that can be performed with the arrangement, is titration of heparin with protamine. Methods of assembly and use are provided.
    Type: Application
    Filed: February 28, 2014
    Publication date: March 5, 2015
    Inventor: Kee Van Sin
  • Publication number: 20140323908
    Abstract: The invention relates to a method for detecting a foreign cell in a tissue of the upper intestinal tract, sold method comprising: (a) providing a sample of cells from the epithelium layer of the tissue of interest; (b) preparing the cells into a cohesive cluster; (c) staining cells from the cohesive cluster using haematoxylin and eosin (H&E) stain; (d) observing the stained cells; and (e) inferring the presence or absence of foreign cell(s) from the observations of Id), The invention also relates to a method for aiding the diagnosis of an inflammatory condition of the upper intestinal tract in a subject, said method comprising detecting a foreign cell as described above, wherein detection of foreign cell(s) in step (e) infers that the subject has an inflammatory condition of the upper intestinal tract.
    Type: Application
    Filed: May 22, 2014
    Publication date: October 30, 2014
    Inventors: REBECCA C. FITZGERALD, MARIA O'DONOVAN
  • Publication number: 20140303032
    Abstract: Systems and methods of polynucleotide sequencing are provided. Systems and methods optimize control, speed, movement, and/or translocation of a sample (e.g., a polynucleotide) within, through, or at least partially through a nanopore or a type of protein or mutant protein in order to accumulate sufficient time and current blocking information to identify contiguous nucleotides or plurality of nucleotides in a single-stranded area of a polynucleotide.
    Type: Application
    Filed: November 1, 2012
    Publication date: October 9, 2014
    Inventors: Timothy Geiser, Randall Davis, Roger Chen
  • Patent number: 8753863
    Abstract: Disclosed is a method for removing carbon dioxide from the atmosphere. The method comprises the step of delivering urea from a floating vessel to a region of a photic zone of the ocean, whereby the number of phytoplankton is caused to increase in the region upon addition of the urea. The method can be used for producing carbon cells.
    Type: Grant
    Filed: June 22, 2007
    Date of Patent: June 17, 2014
    Assignee: Ocean Nourishment Corporation Pty Limited
    Inventors: Ian Stanley Ferguson Jones, William Rodgers, Robert John Wheen, Bruce Joseph Judd
  • Publication number: 20140030747
    Abstract: A sensor system for detecting a chemical or biological species includes a sensing element and a bias and measurement circuit. The sensing element includes nanochannels having an outer surface functionalized for interaction with the species to create a surface potential, and each having a sufficiently small cross section to exhibit a shift of differential conductance into a negative bias operating region by a shift amount dependent on the surface potential. The bias and measurement circuit applies a bias voltage across two ends of the nanochannels sufficiently negative to achieve a desired dependence of the differential conductance on the surface potential. The dependence has a steeply sloped region of high amplification substantially greater than a reference amplification at a zero-bias condition, thus achieving relatively high signal-to-noise ratio. The bias and measurement circuit converts the measured differential conductance into a signal indicative of presence or activity of the species.
    Type: Application
    Filed: April 30, 2012
    Publication date: January 30, 2014
    Inventors: Yu Chen, Xihua Wang, Agniezska Kalinowski, Mi Hong, Pritiraj Mohanty, Shyamsunder Erramilli
  • Publication number: 20130273583
    Abstract: Detection of urease in gastric samples is achieved by using a composition comprising urea and an indicator. This composition can be provided in the form of a liquid or in a number of dry formats including a wafer, impregnated material, coated well or a tablet. The assay can be performed in described apparatus comprising a well which can be provided with a removable air-tight seal. In one optional form, the well may have a swaged rim for retaining a disc or ring inside the well and hinged plugs. This assay is useful for detecting H.
    Type: Application
    Filed: October 17, 2011
    Publication date: October 17, 2013
    Applicant: CENTRE FOR DIGESTIVE DISEASES PTY LIMITED
    Inventor: Thomas Julius Borody
  • Publication number: 20130149727
    Abstract: A method for isolating urea and removing CO2 from plasma samples, comprising the following steps: a) providing a plasma sample; b) adding an acid so as to partially remove CO2; c) lyophilizing the sample so as to further remove CO2 and obtain a dried sample; and d) redissolving the dried sample and neutralizing to a pH value of 4 to 7 using a buffer solution, wherein optionally a filtration step is carried out before adding the acid.
    Type: Application
    Filed: July 30, 2011
    Publication date: June 13, 2013
    Applicant: Cytonet GmbH & Co. KG
    Inventor: Sitke Aygen
  • Patent number: 8343122
    Abstract: The invention provides for a urinary monitoring device to monitor for the presence or absence of markers indicative of a urinary tract infection (UTI). The invention also provides for methods of using such a device.
    Type: Grant
    Filed: April 23, 2010
    Date of Patent: January 1, 2013
    Inventor: Geoffrey H Gorres
  • Patent number: 8268590
    Abstract: Non-activated tissue-regeneration polypeptides (TRPs) and their preparation methods are disclosed. The TRPs include: a protein transduction domain (PTD) making the polypeptides to permeate a cell membrane without cell membrane receptors; a furin activation domain (FAD) which has at least one proprotein convertase cleavage site and which can be cleaved by the proprotein convertase and activate a tissue regeneration domain (TRD) in cells; and a tissue regeneration domain (TRD) which can be activated by the proprotein convertase cleavage of the FAD to stimulate the growth or formation of tissues or to induce the regeneration of tissues. The TRPs can be mass-produced by cultured bacteria, such as recombinant E. coli, are in a non-activated state before in vivo administration, and their separation, purification, handling, storage and administration are simple and convenient.
    Type: Grant
    Filed: July 21, 2007
    Date of Patent: September 18, 2012
    Inventors: Jung Moon Kim, Jung Kook Kim, Tae Han Kim, Jong Suk Lee, Jong In Yook
  • Patent number: 8236517
    Abstract: A BUN (blood urea nitrogen) sensor containing immobilized carbonic anhydrase and immobilized urease for the in vitro detection of urea nitrogen in blood and biological samples with improved performance and precision characteristics.
    Type: Grant
    Filed: February 25, 2009
    Date of Patent: August 7, 2012
    Assignee: Abbott Point of Care Inc.
    Inventors: G. Bruce Collier, Eric Brouwer, Anjulia Wong
  • Patent number: 8182663
    Abstract: A BUN (blood urea nitrogen) sensor containing immobilized carbonic anhydrase and immobilized urease for the in vitro detection of urea nitrogen in blood and biological samples with improved performance and precision characteristics.
    Type: Grant
    Filed: February 25, 2009
    Date of Patent: May 22, 2012
    Assignee: Abbott Point of Care Inc.
    Inventors: G. Bruce Collier, Eric Brouwer, Anjulia Wong
  • Publication number: 20120040383
    Abstract: Embodiments of the present invention provide methods of assessing metabolite biomarkers to diagnose, monitor, prognose and treat colorectal cancer, as well as kits and systems in use thereof. In some embodiments, the metabolite biomarker profile of an subject suspected of having colorectal cancer may be compared to the metabolite biomarker profile of a healthy subject to determine if there are differences between the profiles indicative of colorectal cancer. In some embodiments, the stage of a subject's colorectal cancer may be determined. In certain embodiments, the metabolite biomarker profile of an subject may be useful in determining method of treatment for colorectal cancer. In other embodiments, the metabolite biomarker profiles of an subject having colorectal cancer may be compared prior to and after treatment to determine if there are differences between the profiles indicative of decreased colorectal cancer morbidity.
    Type: Application
    Filed: August 12, 2011
    Publication date: February 16, 2012
    Inventors: Wei Jia, Yunping Qiu
  • Patent number: 8101062
    Abstract: A method for determining a concentration of an analyte is disclosed. The method includes applying a potential excitation to a fluid sample containing an analyte and determining if a current decay curve associated with the fluid sample has entered an analyte depletion stage. The method also includes measuring a plurality of current values associated with the fluid sample during the analyte depletion stage and calculating an analyte concentration based on at least one of the plurality of current values.
    Type: Grant
    Filed: March 24, 2009
    Date of Patent: January 24, 2012
    Assignee: Nipro Diagnostics, Inc.
    Inventor: David Deng
  • Publication number: 20110287461
    Abstract: The invention is directed to a novel enzymatic analytical membrane, a lateral flow enzymatic detection method, and analytical device incorporating same. The invention is useful for rapidly enzymatically determining the presence of one or more analytes in small volumes of sample. The invention provides an enzymatic analytical membrane for detecting the presence of one or more small-molecule analytes in a biological sample where the membrane comprises a receiving zone; a separation zone and a signal zone, at least one of the zones comprising one or more enzymes for converting the analytes into a form detectable by reaction with a chromogenic agent present in the signal zone and wherein the membrane horizontally receives sample at the receiving zone, and the sample continues via lateral flow through the receiving zone, separation zone and signal zone where a visible color change is formed indicating the presence of the analyte.
    Type: Application
    Filed: May 26, 2009
    Publication date: November 24, 2011
    Applicant: ZBX CORPORATION
    Inventor: Qinwei Shi
  • Patent number: 7960132
    Abstract: A measuring device (100a) includes a base body (101) constituting: a sample holding portion (102) configured to hold a test sample containing urea and a material to be detected; and a sample supply port (103) through which the test sample is supplied to the sample holding portion (102). The base body includes an optical measuring portion and a reagent holding portion (111) which are configured to carry out an optical measurement of the test sample held by the sample holding portion (102). The reagent holding portion holds an antibody to the material to be detected and urease which causes hydrolysis of the urea. With this, the present invention provides a measuring device, a measuring apparatus, and a measuring method, each having a simple configuration and capable of reducing measurement errors caused by the urea contained in the test sample and accurately measuring the material to be detected.
    Type: Grant
    Filed: February 20, 2009
    Date of Patent: June 14, 2011
    Assignee: Panasonic Corporation
    Inventors: Takahiro Nakaminami, Jin Muraoka
  • Patent number: 7947467
    Abstract: A method for identifying physiological conditions associated with the pH or the buffer capacities of biological fluid secreted from a person, by providing a secretion-monitoring article of a body that includes an absorbent material for absorbing a biological fluid secreted from a person and an indicator system having a hydrophobic chemical composition that includes an indicator agent and an ion-balance reagent, at specific relative amounts. Optionally, a competitive agent for establishing a pre-set threshold of an analyte of interest in a tested bodily fluid, is added to the indicator system, wherein the indicator reagent being charged oppositely to the analyte of interest and the competitive reagent having the same charge as the indicator reagent.
    Type: Grant
    Filed: May 27, 2009
    Date of Patent: May 24, 2011
    Assignee: Common Sense Ltd.
    Inventors: Amnon Kritzman, Nitsa G. Nachshon, Yael Behar, David Brusilovsky, Menashe Terem, Adva Brand
  • Patent number: 7931920
    Abstract: A composition suitable for the isolation of a nucleic acid from a material containing the nucleic acid contains at least one buffer with a chaotropic component; at least one proteolytic enzyme; at least one buffer with an non-chaotropic component; at least one alcoholic component; and a detergent.
    Type: Grant
    Filed: May 28, 2008
    Date of Patent: April 26, 2011
    Assignee: AJ Innuscreen GmbH
    Inventor: Timo Hildebrand
  • Patent number: 7833741
    Abstract: The present invention provides an advance in phage display technology by permitting the uncoupling of the propagation of phages containing inserted sequences encoding heterologous polypeptides from the expression of said polypeptides. The invention provides phage constructs and methods for their use to permit phage coat protein expression, and thus phage propagation, in the absence of display of heterologous polypeptides, which may be expressed as a fusion with said coat protein in a regulated manner.
    Type: Grant
    Filed: September 22, 2006
    Date of Patent: November 16, 2010
    Assignee: Ambit Biosciences Corporation
    Inventors: Pietro Ciceri, Patrick Parvis Zarrinkar, Daniel Kelly Treiber, David J. Lockhart
  • Publication number: 20100227345
    Abstract: An aqueous enzyme based sensor comprising one or more enzymes and at least one indicator compound capable of indicating a reaction of at least one of the enzymes with at least one target analyte, wherein the enzyme and the indicator are capable of being delivered, preferably by being sprayed, to a surface having the target analyte without sampling the surface. An aqueous enzyme based sensor is provided that is capable of detecting an enzyme specific substrate as the analyte. This aqueous enzyme based sensor further comprises of one or more enzyme specific substrates in which the sensor detects enzyme inhibition. The aqueous enzyme based sensor optionally comprises one or more of a light scattering additive(s), an adhesive polymer(s), a protein stabilizer(s), a protein stabilizing sugar(s), a surfactant(s), and a solvent(s), and combinations thereof. A dispensing system and a method of detecting a target chemical on a surface without sampling the surface is provided.
    Type: Application
    Filed: August 9, 2006
    Publication date: September 9, 2010
    Inventors: Keith E. LeJuene, Jennifer A. Bacior, Jason A. Berberich, Markus Erbeldinger, Bhalchandra Shripad Lele
  • Publication number: 20100221762
    Abstract: A method and apparatus are described that permit an analyte concentration to be estimated from a measurement in the presence of compounds that interfere with the measurement. The method reduces the error in the analyte concentration in the presence of interferents. The method includes the use of a set of measurements obtained for a large population having a range of known analyte and interfering compound concentrations. From a sample measurement, which may or may not be one of the population, likely present interferents are identified, and a calibration vector is calculated.
    Type: Application
    Filed: October 5, 2009
    Publication date: September 2, 2010
    Applicant: OPTISCAN BIOMEDICAL CORPORATION
    Inventors: Bernhard B. Sterling, W. Dale Hall, Kenneth G. Witte, Mark Wechsler, Peng Zheng, Richard Keenan
  • Patent number: 7727206
    Abstract: The invention provides for a urinary monitoring device to monitor for the presence or absence of markers indicative of a urinary tract infection (UTI). The invention also provides for methods of using such a device.
    Type: Grant
    Filed: December 27, 2005
    Date of Patent: June 1, 2010
    Inventor: Geoffrey H. Gorres
  • Publication number: 20100129786
    Abstract: Disclosed herein are agents, methods, and kits for determining the presence or concentration of a target, or multiple targets, in a sample, in a uniplexed or multiplexed fashion. In general, the methods enable the analysis of small molecules produced or consumed in liquid-phase that may be analyzed using gas or vapor phase detection methods.
    Type: Application
    Filed: November 21, 2008
    Publication date: May 27, 2010
    Applicant: General Electric Company
    Inventors: Andrew David Pris, Frank John Mondello
  • Publication number: 20100047833
    Abstract: The present invention relates to a diagnostic method for the determination of Helicobacter pylori in a tissue biopsy comprising the step of incubating a tissue biopsy with an aqueous diagnostic reagent comprising urea and a pH indicator, and determining any pH change, characterised in that said diagnostic reagent comprises a lactone, and that the ratio between the volume of the reagent and the volume of the sample is variable and less than 30.
    Type: Application
    Filed: March 7, 2008
    Publication date: February 25, 2010
    Inventor: Paolo Giordano
  • Patent number: 7662637
    Abstract: Methods and apparatus (FIG. 4a) are disclosed for selective and very sensitive detection of certain hydrolyzable compounds, especially urea, in water by hydrolyzing said hydrolyzable compounds in a sample of the water to one or more carbon dioxide group compounds and determining the difference in the carbon dioxide content of the water and the hydrolyzed sample using conductivity measurements or other carbon dioxide detector outputs.
    Type: Grant
    Filed: December 2, 2002
    Date of Patent: February 16, 2010
    Assignee: GE Analytical Instruments
    Inventor: Richard D. Godec
  • Patent number: 7595199
    Abstract: The present invention relates to methods of detecting the presence of detergent- or urea-insoluble amyloid-like fibrils or protein aggregates on filters. Preferably, said fibrils or aggregates are indicative of a disease, preferably of a neurodegenerative disease such as Alzheimer's disease or Huntington's disease. In addition, the present invention relates to inhibitors identified by the method of the invention, to pharmaceutical compositions comprising said inhibitors and to diagnostic compositions useful for the investigation of said amyloid-like fibrils or aggregates.
    Type: Grant
    Filed: July 31, 1998
    Date of Patent: September 29, 2009
    Assignee: Max-Planck-Gesellschaft zur Förderung der Wissenchaften e.V.
    Inventors: Erich Wanker, Hans Lehrach, Eberhard Scherzinger, Gillian Bates
  • Patent number: 7582485
    Abstract: The invention provides a breath testing device which visually indicates the presence of ammonia in a patient's breath, in particular ammonia from helicobacter pylori urease infection. The breath testing device comprises a visual indicating agent which changes color in response to ammonia odors, such as 4,4?-bis(dimethylamino)-benzhydrol (Michler's hydrol or BDMB), pararosaniline base and alpha-naphtholbenzein. The indicating agent is applied to a substrate which is then inserted into a tube or straw, which can be attached to the inlet of a breath collection balloon. When the patient blows into the tube or straw, the indicating agent will change color if it detects levels of ammonia which are consistent with helicobacter pylori urease infection.
    Type: Grant
    Filed: October 16, 2003
    Date of Patent: September 1, 2009
    Assignee: Kimberly-Clark Worldride, Inc.
    Inventors: RameshBabu Boga, John Gavin MacDonald
  • Publication number: 20090181412
    Abstract: Provided is a method of storing a reagent in a microfluidic device. The reagent, in a liquid form, is loaded into reaction chambers arranged on the microfluidic device; followed by lyophilization as being contained in the microfluidic device.
    Type: Application
    Filed: December 11, 2008
    Publication date: July 16, 2009
    Applicant: SAMSUNG ELECTRONICS CO., LTD.
    Inventors: Yoon-kyoung CHO, Hee-Kyun LIM, Jeong-gun LEE, Ki-eun KIM, Sung-ho LEE, Chae-joon LEE
  • Publication number: 20090170140
    Abstract: A BUN (blood urea nitrogen) sensor containing immobilized carbonic anhydrase and immobilized urease for the in vitro detection of urea nitrogen in blood and biological samples with improved performance and precision characteristics.
    Type: Application
    Filed: February 25, 2009
    Publication date: July 2, 2009
    Applicant: ABBOTT POINT OF CARE INC.
    Inventors: G. Bruce Collier, Eric Brouwer, Anjulia Wong
  • Publication number: 20090162879
    Abstract: A measuring device (100a) includes a base body (101) constituting: a sample holding portion (102) configured to hold a test sample containing urea and a material to be detected; and a sample supply port (103) through which the test sample is supplied to the sample holding portion (102). The base body includes an optical measuring portion and a reagent holding portion (111) which are configured to carry out an optical measurement of the test sample held by the sample holding portion (102). The reagent holding portion holds an antibody to the material to be detected and urease which causes hydrolysis of the urea. With this, the present invention provides a measuring device, a measuring apparatus, and a measuring method, each having a simple configuration and capable of reducing measurement errors caused by the urea contained in the test sample and accurately measuring the material to be detected.
    Type: Application
    Filed: February 20, 2009
    Publication date: June 25, 2009
    Applicant: PANASONIC CORPORATION
    Inventors: Takahiro Nakaminami, Jin Muraoka
  • Publication number: 20090143487
    Abstract: This invention is directed to the application of a previously unknown property of nanomaterials—its ability to enhance protein activity and stability at high temperatures, in organic solvents, and in polymer composites. Nanomaterials such as single-walled carbon nanotubes (SWNTs) can significantly enhance enzyme function and stability in strongly denaturing environments. Experimental results and theoretical analysis reveal that the enhancement in stability is a result of the curvature of these nanoscale materials, which suppresses unfavorable protein-protein interactions. The enhanced stability is also exploited in the preparation of highly stable and active nanocomposite films that resist nonspecific protein absorption, i.e., inhibit fouling of the films. The protein-nanoparticles conjugates represent a new generation of highly selective, active, and stable catalytic materials.
    Type: Application
    Filed: September 7, 2005
    Publication date: June 4, 2009
    Inventors: Jonathan S. Dordick, Ravindra S. Kane, Prashanth Asuri, Sandeep S. Karajanagi