Measuring Or Testing Process Involving Enzymes Or Micro-organisms; Composition Or Test Strip Therefore; Processes Of Forming Such Composition Or Test Strip Patents (Class 435/4)
  • Patent number: 11326146
    Abstract: The present invention relates to compositions comprising low sugar media, the methods of using these compositions to reduce trauma and stress on processed animal sperm, the resulting sperm and embryo products, and the methods of use of these products to increase the quality, quantity and viability of progeny and improved rates of births in animals.
    Type: Grant
    Filed: November 20, 2015
    Date of Patent: May 10, 2022
    Assignee: Inguran, LLC
    Inventors: Thomas B. Gilligan, Ramakrishnan Vishwanath, Kilby Willenburg
  • Patent number: 11320442
    Abstract: Disclosed is a method for body fluid-based neurodegenerative disease diagnosis through high-sensitive immunoassay of aggregated proteins by photooxidation-induced amplification. The method according to the present disclosure provides an effect of quantitatively analyzing aggregated proteins in the form of oligomers or monomers which are present in trace amounts in a body fluid and measures normal or abnormal protein aggregation by detecting the aggregated proteins in the form of oligomers or monomers with high sensitivity by reaction of antibody-conjugated enzymes selectively bound to the aggregated proteins with substrates and photooxidation-induced amplification, thereby allowing accurate diagnosis of a neurodegenerative disease.
    Type: Grant
    Filed: April 19, 2017
    Date of Patent: May 3, 2022
    Assignee: KOREA INSTITUTE OF SCIENCE AND TECHNOLOGY
    Inventors: Ji Yoon Kang, Min Cheol Park, Youhee Heo
  • Patent number: 11304686
    Abstract: A guidance system and method include a real-time imaging device (121) configured to intra-operatively collect image data of a target region to provide a real-time image. A target determination module (108) is configured to generate an overlay map (111) registered to the real-time image to project morphological feature information on corresponding features in the real-time image of the target region. A medical instrument (122) is provided to perform a procedure on the target region such that the at least one medical instrument is guided to a region suitable for performing an operative task based upon the overlay map.
    Type: Grant
    Filed: June 11, 2012
    Date of Patent: April 19, 2022
    Assignee: KONINKLIJKE PHILIPS N.V.
    Inventors: Robert Manzke, Raymond Chan, Haytham Elhawary, Aleksandra Popovic
  • Patent number: 11310239
    Abstract: A network connection method, a hotspot terminal, and a management terminal are provided. A hotspot terminal receives an Internet access request sent by a mobile terminal, sends an input request to the mobile terminal according to the received Internet access request; receives user identity information sent by the mobile terminal; receives a permission confirmation instruction that is entered by an administrator according to the user identity information; determines Internet access permission of the mobile terminal according to the received permission confirmation instruction. According to the network connection method provided in the embodiments of the present application, the tedious and complex process where a mobile terminal logging into a remote network by using a hotspot terminal is significantly improved.
    Type: Grant
    Filed: February 4, 2020
    Date of Patent: April 19, 2022
    Assignee: Huawei Technologies Co., Ltd.
    Inventor: Zhen Zhong
  • Patent number: 11298061
    Abstract: A specimen mixing and transfer device adapted to receive a sample is disclosed. The specimen mixing and transfer device includes a housing, a material including pores that is disposed within the housing, and a dry anticoagulant powder within the pores of the material. In one embodiment, the material is a sponge material. In other embodiments, the material is an open cell foam. In one embodiment, the material is treated with an anticoagulant to form a dry anticoagulant powder finely distributed throughout the pores of the material. A blood sample may be received within the specimen mixing and transfer device. The blood sample is exposed to and mixes with the anticoagulant powder while passing through the material.
    Type: Grant
    Filed: September 22, 2015
    Date of Patent: April 12, 2022
    Assignee: Becton, Dickinson and Company
    Inventors: Milan Ivosevic, Alexander James Blake, Ryan W. Muthard
  • Patent number: 11301536
    Abstract: The present disclosure is directed to associating computing devices with each other based on computer network activity for selection of content items as part of an online content item placement campaign. A first linking factor is identified based on a connection between a first device and the computer network via a first IP address during a first time period, and based on a connection between a second device and the computer network via the first IP address during the first time period. A number of devices that connect with the computer network via the first IP address is determined. A positive match probability is generated. A second and third linking factors are monitored. A negative match probability is determined based on the second and third linking factors. The first device is linked with the second device based on the positive and negative match probabilities.
    Type: Grant
    Filed: March 6, 2020
    Date of Patent: April 12, 2022
    Assignee: GOOGLE LLC
    Inventor: Jianjun Qiu
  • Patent number: 11293061
    Abstract: Embodiments of the present disclosure relate to sequencing methods using nucleotide molecules with a 3? AOM blocking group, which can provide reduced phasing and pre-phasing values. Also provided herein are kits related to such sequencing methods.
    Type: Grant
    Filed: December 20, 2019
    Date of Patent: April 5, 2022
    Assignee: Illumina Cambridge Limited
    Inventors: Antoine Francais, Elena Cressina, Adam Culley, Angelica Mariani, Xiaolin Wu, Xiaohai Liu
  • Patent number: 11285475
    Abstract: A device and method for fluorescent labelling a component is provided. The device comprising a first user-replaceable reservoir comprising a strongly buffered alkaline solution of aromatic ortho-dialdehyde dye, a second user-replaceable reservoir comprising a weakly buffered acidic solution of a reducing agent, one or more fluid pathways comprising the component, and a network of connection channels linking the reservoirs and the fluid pathway to enable the alkaline solution and the acidic solution to combine with the component in order to label the component by reacting the component with the alkaline solution and the acidic solution.
    Type: Grant
    Filed: September 11, 2017
    Date of Patent: March 29, 2022
    Assignee: Fluidic Analytics Limited
    Inventors: Tuomas Pertti Jonathan Knowles, Sean Devenish
  • Patent number: 11278657
    Abstract: Cerebrospinal fluid (CSF) and other fluid amelioration systems completely or partially implantable within a mammalian subject and associated methods include a substrate and an agent for amelioration of a toxic biomolecule present in the CSF or fluid, wherein the agent is disposed on or within the substrate.
    Type: Grant
    Filed: October 2, 2020
    Date of Patent: March 22, 2022
    Assignee: EnClear Therapies, Inc.
    Inventors: Anthony DePasqua, Kevin Eggan, Jr., Kevin Kalish, Manuel A. Navia, Kasper Roet, Ching-Hua Tseng, Alan D. Watson, William X. Siopes, Gianna N. Riccardi, Marcie Ann Glicksman
  • Patent number: 11280796
    Abstract: The present invention relates to methods of diagnosing samples as well as various microfluidic, microcentrifuge and microfilter devices. In one embodiment, the present invention provides a method of diagnosing neurodegenerative diseases using mitochondrial and/or platelet samples. In another embodiment, the present invention provides a microfluidic device that selectively captures and analyzes a desired amount of target biological particle.
    Type: Grant
    Filed: April 2, 2019
    Date of Patent: March 22, 2022
    Assignee: Dignity Health
    Inventor: Jonathan E. Valla
  • Patent number: 11275080
    Abstract: A method of analyzing a biological sample from a subject that has a tumor or cancer, comprising: determining, for target cells having a phenotype of interest spatial resolution of the target cells, density of the spatially resolved target cells in the sample; and proximity between spatially resolved target cells of interest in the sample; and determining an overall score based at least in part on the preceding parameters. A method for identifying a patient as a responder to single agent anti-PD-1 or anti-PD-L1 therapy is provided. Similar methods are provided for detecting adaptive immune resistance, the presence of cancer in a patient sample, determining efficacy of cancer therapy, and determining response to and monitoring the efficacy of cancer therapy.
    Type: Grant
    Filed: November 5, 2015
    Date of Patent: March 15, 2022
    Assignee: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
    Inventors: Paul C. Tumeh, Antoni Ribas
  • Patent number: 11276482
    Abstract: The present invention provides a device and system for monitoring the accuracy of procedures in the course of the performance of a task, the task comprising at least one procedure to be performed, the device comprising: an input interface for receiving input data relating to the procedures; a data store for storing data relating to the procedures; a processor for: comparing the input data with the stored data; and generating a comparison result indicating the result of that comparison; and an output interface for outputting the comparison result.
    Type: Grant
    Filed: June 14, 2016
    Date of Patent: March 15, 2022
    Assignee: GENEA IP HOLDINGS PTY LIMITED
    Inventors: Eduardo Vom, Jeremy Phillip Stimson, Yassaman Pouladi, Ben Hobbs, Tammie Kim Roy, Jan Kirsten
  • Patent number: 11261454
    Abstract: The present invention relates to a method for introducing a polynucleotide into non-adhesively growing plant cells, comprising the following steps: providing a solid support having immobilized thereto the polynucleotide in dry state; contacting the plant cells with the polynucleotide on the solid support so as to obtain transformed plant cells; and optionally washing the plant cells.
    Type: Grant
    Filed: April 9, 2019
    Date of Patent: March 1, 2022
    Assignee: ScreenSYS GmbH
    Inventors: Oleksandr Dovzhenko, Claude Becker, Karsten Voigt, Klaus Palme
  • Patent number: 11261487
    Abstract: The claimed invention relates to a method of processing a polynucleotide, by obtaining a sense polynucleotide strand comprising a homopolymeric region that is longer that the reading section of a nanopore; synthesizing an antisense polynucleotide strand under conditions in which a nucleotide analog is incorporated at random in a reverse complement of the homopolymer region, such that the length of the homopolymer region in the antisense polynucleotide strand is shorter than the reading section of the nanopore; and moving the antisense polynucleotide strand through the nanopore such that a proportion of the antisense polynucleotide strand interacts with the nanopore.
    Type: Grant
    Filed: February 5, 2020
    Date of Patent: March 1, 2022
    Assignee: Oxford Nanopore Technologies PLC
    Inventors: Clive Gavin Brown, James Anthony Clarke, Graham Hall, Gavin Harper, Andrew John Heron, James White
  • Patent number: 11256994
    Abstract: A system and method that predicts whether a given protein-ligand pair is active or inactive and outputs a pose score classifying the propriety of the pose. A 3D bioactivity platform comprising a 3D bioactivity module and data platform scrapes empirical lab-based data that a docking simulator uses to generate a dataset from which a 3D-CNN model is trained. The model then may receive new protein-ligand pairs and determine a classification for the bioactivity and pose propriety of that protein-ligand pair. Furthermore, gradients relating to the binding affinity in the 3D model of the molecule may be used to generate profiles from which new protein targets may be determined.
    Type: Grant
    Filed: March 16, 2021
    Date of Patent: February 22, 2022
    Assignee: RO5 INC.
    Inventors: Alwin Bucher, Aurimas Pabrinkis, Orestis Bastas, Mikhail Demtchenko, Zeyu Yang, Cooper Stergis Jamieson, {hacek over (Z)}ygimantas Jo{hacek over (c)}ys, Roy Tal, Charles Dazler Knuff
  • Patent number: 11255809
    Abstract: The present invention relates to droplet-based surface modification and washing. According to one embodiment, a method of splitting a droplet is provided, the method including providing a droplet microactuator including a droplet including one or more beads and immobilizing at least one of the one or more beads. The method further includes conducting one or more droplet operations to divide the droplet to yield a set of droplets including a droplet including the one or more immobilized beads and a droplet substantially lacking the one or more immobilized beads.
    Type: Grant
    Filed: February 11, 2020
    Date of Patent: February 22, 2022
    Assignees: Advanced Liquid Logic, Inc., Duke University
    Inventors: Vamsee K. Pamula, Vijay Srinivasan, Michael G. Pollack, Richard B. Fair
  • Patent number: 11249070
    Abstract: A method of assessing an anti-cancer drug including culturing a cell structure including cancer cells and stromal cells in a presence of at least one anti-cancer drug, and assessing an anti-cancer effect of the at least one anti-cancer drug based on a number of viable cancer cells in the cell structure after the culturing.
    Type: Grant
    Filed: October 19, 2018
    Date of Patent: February 15, 2022
    Assignee: TOPPAN PRINTING CO., LTD.
    Inventors: Shiro Kitano, Kei Tsukamoto, Shinji Irie
  • Patent number: 11224586
    Abstract: The present invention relates to a compound of the following general formula (I); or a pharmaceutically acceptable salt and/or solvate thereof, for use as drug, particularly intended for inhibiting a programmed cell death route selected from the group consisting of ferroptosis, oxytosis and cellular necroptosis. The present invention also relates to a compound of general formula (I) for use as a drug for neuroprotection as well as for preventing and/or treating disorders associated with cellular necroptosis or ferroptosis. The present invention also relates to a pharmaceutical composition comprising a compound of general formula (I), or a pharmaceutically acceptable salt and/or solvate thereof. The present invention also encompasses the use of a compound of the general formula (I) for organs preservation.
    Type: Grant
    Filed: October 18, 2017
    Date of Patent: January 18, 2022
    Assignees: Institut National de la Sante et de la Recherche Medicale (INSERM), Centre National de la Recherche Scientifique (CNRS), Sorbonne Universite, Universite de Poitiers, Centre Hospitalier Universitaire de Poitiers
    Inventors: Stéphane Bach, Marie-Thérèse Dimanche-Boitrel, Claire Delehouze, Thierry Hauet
  • Patent number: 11215608
    Abstract: The present invention provides methods of determining cell sensitivity to a therapeutic agent.
    Type: Grant
    Filed: October 26, 2016
    Date of Patent: January 4, 2022
    Assignee: Dana-Farber Cancer Institute, Inc.
    Inventor: Anthony Letai
  • Patent number: 11208698
    Abstract: Embodiments of this invention include methods for detection of markers of bladder cancer and inflammatory conditions of the bladder. Particularly, methods include detection of expression of certain genetic markers for bladder cancer and markers for detection of inflammatory conditions of the bladder. These methods provide improved detection of the markers, and provide better detection of bladder cancer and inflammatory conditions of the bladder.
    Type: Grant
    Filed: May 24, 2018
    Date of Patent: December 28, 2021
    Assignee: PACIFICEDGE LIMITED
    Inventors: Parry John Guilford, Mark Dalphin, Laimonis Kavalieris, Paul O'Sullivan
  • Patent number: 11203016
    Abstract: In accordance with embodiments herein a method for capturing cells of interest in a digital microfluidic system is provided, comprising utilizing a droplet actuator to transport a sample droplet to a microwell device. The microwell device includes a substrate having a plurality of microwells that open onto a droplet operations surface of the microwell device. The sample droplet includes cells of interest that enter the microwells. The method introduces capture beads to the microwells, and the capture elements are immobilized on the capture beads. The method utilizes the droplet actuator to transport a cell lysis reagent droplet to the microwell device. Portions of the cell lysis reagent droplet enter the microwells and, during an incubation period, cause the cells of interest to release analyte that is captured by the capture elements on the capture beads.
    Type: Grant
    Filed: November 30, 2016
    Date of Patent: December 21, 2021
    Assignee: Illumina, Inc.
    Inventors: Arash Jamshidi, Yan-you Lin, Farnaz Absalan, Sarah Stuart, Gordon Cann, Yir-Shyuan Wu, Tarun Khurana, Jeffrey S Fisher
  • Patent number: 11203015
    Abstract: The present invention relates to a microfluidic device for creating within a cell assembly a cell-free area, comprising at least one cell chamber, wherein the at least one cell chamber comprises: —a fluid inlet for introducing fluid into the cell chamber, —a first area, —a second area, —at least one mechanical excluding means for excluding cells from the first area of the chamber and being operable between an excluding position and a releasing position optionally via an actuation line, wherein the second area of the cell chamber is outside of the operation range of the mechanical excluding means.
    Type: Grant
    Filed: April 12, 2017
    Date of Patent: December 21, 2021
    Assignee: TECHNISCHE UNIVERSITAT WIEN
    Inventors: Drago Sticker, Peter Ertl, Sarah Lechner
  • Patent number: 11200446
    Abstract: Methods and systems for image analysis are provided, and in particular for identifying a set of base-calling locations in a flow cell for DNA sequencing. These include capturing flow cell images after each sequencing step performed on the flow cell, and identifying candidate cluster centers in at least one of the flow cell images. Intensities are determined for each candidate cluster center in a set of flow cell images. Purities are determined for each candidate cluster center based on the intensities. Each candidate cluster center with a purity greater than the purity of the surrounding candidate cluster centers within a distance threshold is added to a template set of base-calling locations.
    Type: Grant
    Filed: March 31, 2021
    Date of Patent: December 14, 2021
    Assignee: Element Biosciences, Inc.
    Inventors: Chunhong Zhou, Semyon Kruglyak, Francisco Garcia, Minghao Guo, Haosen Wang, Ryan Kelly
  • Patent number: 11181525
    Abstract: A system and method for using new biomarkers to assess individual diseases is provided. In one embodiment of the present invention, absolute quantification of annotated metabolites by mass spectrometry is used to identify certain biomarkers and derivatives thereof (i.e., signatures), which are then used to screen for, diagnose, predict, prognose, and treat various diseases, including, but not limited to, breast cancer, ovarian cancer, colorectal cancer, pancreatic cancer, and acute graft-versus-host disease.
    Type: Grant
    Filed: December 16, 2019
    Date of Patent: November 23, 2021
    Assignee: Metabolomyes, Inc.
    Inventors: Robert Nagourney, Ismael Silva, Paulo D'Amora
  • Patent number: 11166938
    Abstract: Provided is an external composition excellent in stability and impression from use. An external composition containing (A) at least one member selected from the group consisting of ascorbic acid and salts of ascorbic acid, (B) at least one member selected from the group consisting of 3-O-ethylascorbic acid, salts of 3-O-ethylascorbic acid, and hydroxylated lecithin, and (C) polyethylene glycol is prepared.
    Type: Grant
    Filed: June 28, 2017
    Date of Patent: November 9, 2021
    Assignee: ROHTO PHARMACEUTICAL CO., LTD.
    Inventors: Masatoshi Haga, Yuya Hayashi
  • Patent number: 11166987
    Abstract: The present invention relates to recombinant virulence attenuated Gram-negative bacterial strains for use in a method of treating a malignant solid tumor in a subject.
    Type: Grant
    Filed: November 17, 2016
    Date of Patent: November 9, 2021
    Assignee: Universitaet Basel
    Inventors: Simon Ittig, Marlise Amstutz, Christoph Kasper, Guy R. Cornelis
  • Patent number: 11169154
    Abstract: Methods and products are provided for determining if a subject having a tumor is at risk of metastasis of the tumor. Specifically, the methods comprise detecting phosphorylated cofilin, and both phosphorylated and non-phosphorylated cofilin; quantifying the phosphorylated cofilin, and the total of phosphorylated and nonphosphorylated cofilin; and determining if a subject having the tumor is likely to experience metastasis of the tumor, based on the ratio of the amount of detected phosphorylated cofilin:total amount of phosphorylated and non-phosphorylated cofilin detected. Further disclosed are the types of tumor metastases that can be determined using the methods provided.
    Type: Grant
    Filed: July 24, 2018
    Date of Patent: November 9, 2021
    Assignees: ALBERT EINSTEIN COLLEGE OF MEDICINE, KING'S COLLEGE LONDON
    Inventors: John Condeelis, Tony Tsz-Cheong Ng, Gregory Weitsman
  • Patent number: 11170501
    Abstract: An image analysis device may include a memory storing learning data for executing image analysis, and may obtain cell image data representing a cell image including a plurality of cell objects, sequentially identify plural pieces of partial image data from the cell image data, sequentially execute a center determination process on each of the plural pieces of partial image data, classify at least one cell corresponding to at least one cell object among a plurality of cell objects by using results of the center determination process on the plural pieces of partial image data and classification data included in learning data, and output a classification result.
    Type: Grant
    Filed: May 9, 2017
    Date of Patent: November 9, 2021
    Inventor: Toru Nagasaka
  • Patent number: 11169108
    Abstract: A biosensor test strip includes a substrate having a read section and a detection section. A surface of the detection section is formed with a protrusion seat and detection channels. The protrusion seat is provided with a notch and grooves located higher than the detection channels, and the grooves are connected to the detection channels correspondingly; in addition, detection reagents are arranged in the detection channels. A lid covers the detection channels and exposes the notch of the protrusion seat to ensure the detection results of each detection channel.
    Type: Grant
    Filed: April 6, 2020
    Date of Patent: November 9, 2021
    Assignee: GUO HOONG INDUSTRY CO., LTD.
    Inventors: Kuo-Tsai Huang, Kuo-Chung Chang
  • Patent number: 11155604
    Abstract: The present invention relates to the preparation of an antibody analogue capable of being activated reversibly, and uses thereof, and provides a fusion protein comprising an inactive first fragment of an antibody analogue is fused to a stimulus-induced dimerization protein.
    Type: Grant
    Filed: December 21, 2016
    Date of Patent: October 26, 2021
    Assignee: INSTITUTE FOR BASIC SCIENCE
    Inventors: Won Do Heo, Yun Ju Lee, Daseuli Yu, Byung Ouk Park
  • Patent number: 11152082
    Abstract: A protein memory cell and a protein memory system are provided. The protein memory cell includes: first and second electrodes disposed to be spaced apart from each other on a micro channel; a gap region defined between the first and second electrodes on the micro channel; an outer region defined as an opposite side to the gap region based on the first or second electrode on the micro channel; and a photosensitive protein changing conductivity between the first and second electrodes while moving between the gap region and the outer region depending on structural conversion of a chromophore.
    Type: Grant
    Filed: February 24, 2020
    Date of Patent: October 19, 2021
    Assignees: HONGIK UNIVERSITY INDUSTRY-ACADEMIA COOPERATION FOUNDATION, MYONGJI UNIVERSITY INDUSTRY AND ACADEMIA COOPERATION FOUNDATION
    Inventors: Minsub Chung, Sangmin Lee, Hwajin Lee, Tran Nguyen Anh Nhung, Dohyun Kim, Nebiyu Getachew Arega, Minh Khang Chau
  • Patent number: 11136361
    Abstract: [Problem to be Solved] The object of the present invention is to develop a method of regulating a target RNA. [Solution] There is provided a fusion protein comprising a functional domain which improves the protein expression level from mRNA and a PPR protein which can bind to a target mRNA in an RNA base-selective or RNA base sequence-specific manner.
    Type: Grant
    Filed: May 30, 2017
    Date of Patent: October 5, 2021
    Assignee: KYUSHU UNIVERSITY, NATIONAL UNIVERSITY CORPORATION
    Inventors: Takahiro Nakamura, Yusuke Yagi
  • Patent number: 11136573
    Abstract: The present invention relates to methods and compositions for the high throughput screening of protein-protein interactions in yeast liquid culture. Protein fusions non-native to yeast may be expressed to replace endogenous sexual agglutination proteins and mediate library-by-library interrogation of protein interactions. The methods and compositions of the invention can be utilized for the characterization of protein interaction networks in high throughput for both binding affinity and specificity, which is crucial for understanding cellular functions, screening therapeutic candidates, and evaluating engineered protein networks.
    Type: Grant
    Filed: November 6, 2020
    Date of Patent: October 5, 2021
    Assignee: University of Washington
    Inventors: David Baker, David Younger, Eric Klavins
  • Patent number: 11130989
    Abstract: An article such as a biosensor having a nonfouling surface thereon is described. The article comprises: (a) a substrate having a surface portion; (b) a linking layer on the surface portion; (c) a polymer layer comprising brush molecules formed on the linking layer; and (d) optionally but preferably, a first member of a specific binding pair (e.g., a protein, peptide, antibody, nucleic acid, etc.) coupled to the brush molecules. The polymer layer is preferably formed by the process of surface-initiated polymerization (SIP) of monomeric units thereon. Preferably, each of the monomeric units comprises a monomer (for example, a vinyl monomer) core group having at least one protein-resistant head group coupled thereto, to thereby form the brush molecule on the surface portion. Methods of using the articles are also described.
    Type: Grant
    Filed: January 16, 2018
    Date of Patent: September 28, 2021
    Assignee: DUKE UNIVERSITY
    Inventor: Ashutosh Chilkoti
  • Patent number: 11125659
    Abstract: An object of the present invention is to provide a novel preservative solution for a heme protein and a method for stabilizing a heme protein, which are effective against denaturation or degradation of a heme protein, and the present invention specifically relates to a preservative solution for a heme protein comprising a disulfonic acid or a salt thereof, and a method for stabilizing a heme protein, which involves bringing a disulfonic acid or a salt thereof into coexistence in a sample comprising a heme protein.
    Type: Grant
    Filed: March 31, 2016
    Date of Patent: September 21, 2021
    Assignee: EIKEN KAGAKU KABUSHIKI KAISHA
    Inventors: Kozue Sakata, Shin Sugo, Ryota Yasui
  • Patent number: 11105745
    Abstract: In some embodiments, a biosensor is provided. The biosensor includes a sensing element and a cover disposed on the sensing element. The sensing element includes: a first substrate embedded with a photodiode; and a filter layer disposed on the substrate. The cover includes: a second substrate; an upper optical element disposed in the second substrate; and an upper reaction well disposed on the upper optical element.
    Type: Grant
    Filed: October 10, 2019
    Date of Patent: August 31, 2021
    Assignee: VISERA TECHNOLOGIES COMPANY LIMITED
    Inventor: Hsin-Yi Hsieh
  • Patent number: 11097269
    Abstract: A microfluidic device includes: a base plate allowing an electromagnetic wave to pass therethrough and having no autofluorescence; a microwell array formed on the base plate and including a wall layer in which a plurality of through-holes are formed in a thickness direction; and a lid member disposed opposite to the base plate in a state of being separated from the wall layer, wherein microwells are formed by the base plate and the through-holes formed in the wall layer, and wherein the wall layer is formed of a material containing a colored component that absorbs an electromagnetic wave of a predetermined wavelength.
    Type: Grant
    Filed: April 4, 2018
    Date of Patent: August 24, 2021
    Assignee: TOPPAN PRINTING CO., LTD.
    Inventors: Keisuke Goto, Yoichi Makino, Akihiro Hoshino
  • Patent number: 11087472
    Abstract: An image processing device includes a processor to detect motion of objects in an observation area on the basis of a captured image. The processor also calculates amounts of each detected motion, and calculates a propagation speed, a propagation direction, or both, of the motion for pulsations of the objects.
    Type: Grant
    Filed: March 1, 2018
    Date of Patent: August 10, 2021
    Assignee: SONY CORPORATION
    Inventors: Takeshi Kunihiro, Tomohiro Hayakawa, Eriko Matsui, Seiji Kobayashi
  • Patent number: 11078511
    Abstract: The present disclosure is directed to the properties of certain glycosyltransferase variants having N-terminal truncation deletions or internal deletions. Any of the mutants disclosed in here exhibit alpha-2,6-sialyltransferase enzymatic activity in the presence of CMP-activated sialic acid as co-substrate, and in the presence of a suitable acceptor site. A fundamental finding documented in the present disclosure is that these enzymes are not only capable of catalyzing transfer of a sialidyl moiety but they are also capable of catalyzing hydrolytic cleavage of terminally bound sialic acid from a glycan.
    Type: Grant
    Filed: June 21, 2017
    Date of Patent: August 3, 2021
    Assignee: Roche Diagnostics Operations, Inc.
    Inventors: Harald Sobek, Michael Greif, Marco Thomann, Sebastian Malik
  • Patent number: 11071488
    Abstract: The present invention relates to the field of cardiology and, more specifically, to a novel algorithm that can be used, in particular, in a method for determining if a drug is likely to induce a cardiac ventricular repolarisation disturbance, based on variations in electrocardiogram data.
    Type: Grant
    Filed: April 12, 2017
    Date of Patent: July 27, 2021
    Assignees: ASSISTANCE PUBLIQUE—HOPITAUX DE PARIS, INSTITUT NATIONAL DE LA SANTÉ ET DE LA RECHERCHE MÉDICALE (INSERM), SORBONNE UNIVERSITÉ
    Inventors: Joe-Elie Salem, David-Alexandre Tregouet, Jean-Sébastien Hulot, Christian Funck-Brentano, Marine Germain
  • Patent number: 11065356
    Abstract: Nanoparticles as described herein are configured to bind to bacterial contaminants, such as Gram positive bacteria, Gram negative bacteria, and endotoxins. The nanoparticles include a core comprising a magnetic material; and a plurality of ligands attached to the core. The ligands include, for example, bis(dipicolylamine) (“DPA”) coordinated with a metal ion, e.g., Zn2+ or Cu2+, to form, e.g., bis-Zn-DPA or bis-Cu-DPA, which can bind to the bacterial contaminants. The nanoparticles can be included in compositions for use in methods and systems to separate bacterial contaminants from liquids, such as liquids, such as blood, e.g., whole or diluted blood, buffer solutions, albumin solutions, beverages for human and/or animal consumption, e.g., drinking water, liquid medications for humans and/or animals, or other liquids.
    Type: Grant
    Filed: July 15, 2019
    Date of Patent: July 20, 2021
    Assignees: Children's Medical Center Corporation, Massachusetts Institute of Technology
    Inventors: Jung-Jae Lee, Kyung Jae Jeong, Daniel S. Kohane
  • Patent number: 11068796
    Abstract: Methods and systems for pruning process execution logs include learning a predictive model from a set of execution traces that characterize a process, where the predictive model determines a likelihood of a given instance reaching a specified outcome; identifying attributes in the predictive model that fall below a threshold measure of relevance to the specified outcome using a processor; and removing the identified attributes from the set of execution traces.
    Type: Grant
    Filed: November 1, 2013
    Date of Patent: July 20, 2021
    Assignee: INTERNATIONAL BUSINESS MACHINES CORPORATION
    Inventors: Geetika T. Lakshmanan, Szabolcs Rozsnyai, Fei Wang
  • Patent number: 11054349
    Abstract: A method for preparation of a dried blood sample for multiplexing of analytes includes the steps of mixing an Internal Standard solution with a first diluent in a vessel, the Internal Standard solution including a plurality of different Internal Standards, adding the dried blood sample to the vessel, sonicating the vessel containing the Internal Standard solution, the first diluent and the dried blood sample, and removing the dried blood sample from the vessel. The Internal Standard solution can include a plurality of Internal Standards. The Internal Standard solution can include at least 15 Internal Standards. The dried blood sample can be generated using less than 50 ?L of blood. The dried blood sample can be generated using less than 10 ?L of blood. The first diluent can include methanol or a mixture of water and methanol.
    Type: Grant
    Filed: April 29, 2020
    Date of Patent: July 6, 2021
    Assignee: ALCALA PHARMACEUTICAL, INC.
    Inventors: David J. Smith, Matthew D. Rifat, Christian Tagwerker, Irfan Baig, Ranulu Samanthi de Zoysa, Eric J. Brunson
  • Patent number: 11047854
    Abstract: Methods of reducing background noise in signal-generating digital assays, such as fluorescent digital immunoassays, using a colorant are disclosed. The methods utilize a binding member that specifically binds to an analyte in a biological sample. The binding member is conjugated to a signal generating compound, e.g., an enzyme, which is dissociated from the binding member. A colorant is added with a signal generating substrate, e.g., a fluorogenic or chromogenic substrate for the enzyme, or after a signal generating substrate to reduce background noise in a fluorescent digital immunoassay. The signal generated between the signal generating compound and the signal generating substrate is detected and correlated to the presence and/or concentration of the analyte.
    Type: Grant
    Filed: February 5, 2018
    Date of Patent: June 29, 2021
    Assignee: ABBOTT JAPAN LLC
    Inventor: Atsuko Futase
  • Patent number: 11028445
    Abstract: The present invention is based on the discovery of a unique 14-gene molecular prognostic signature that is useful for predicting breast cancer metastasis. In particular, the present invention relates to methods and reagents for detecting and profiling the expression levels of these genes, and methods of using the expression level information in predicting risk of breast cancer metastasis.
    Type: Grant
    Filed: June 8, 2018
    Date of Patent: June 8, 2021
    Assignee: Celera Corporation
    Inventors: Kit Lau, Alice Wang
  • Patent number: 11028426
    Abstract: The invention provides a method of preparing a nucleic acid population suitable for RNA sequencing. The method involves amplifying a double-stranded DNA and a poly T sequence by using the DNA constituted of any additional nucleic acid sequence X, poly T sequence, mRNA sequence isolated from a biological sample, poly A sequence and any additional nucleic acid sequence Y in this order as a template, a first primer containing any additional nucleic acid sequence X having amine added to the 5?-terminal (and a poly T sequence), and a second primer containing any additional nucleic acid sequence Y (and a poly T sequence), followed by fractionalizing the DNA, phosphorylating the DNA, preparing cDNA by using the DNA as a template and a third primer, adding adenine (A) to the cDNA, linking a DNA, and amplifying the DNA by using the DNA as a template, a fourth primer, and a fifth primer.
    Type: Grant
    Filed: February 23, 2016
    Date of Patent: June 8, 2021
    Assignee: KYOTO UNIVERSITY
    Inventors: Mitinori Saitou, Tomonori Nakamura, Yukihiro Yabuta
  • Patent number: 10996168
    Abstract: A computer-implemented method includes controlling an instrument to measure a fluorescence emission spectrum of a sample including a first peak emission wavelength and at least a second peak emission wavelength, emitted in response to an excitation wavelength and controlling the instrument to measure an absorbance obtained at the excitation wavelength of the sample. The method may include determining, using the computer, a ratio of the measurements at either the second peak emission wavelength, or a sum of measurements at a plurality of peak emission wavelengths including at least the first peak emission wavelength and the second peak emission wavelength, to the first peak emission wavelength, and calculating, using the computer, a value for a quality parameter based on a combination of at least the ratio and the absorbance measurement. The method may include controlling an associated process based on the quality parameter.
    Type: Grant
    Filed: May 4, 2017
    Date of Patent: May 4, 2021
    Assignee: Horiba Instruments Incorporated
    Inventor: Adam Matthew Gilmore
  • Patent number: 10988759
    Abstract: The present invention relates to methods and compositions for the high throughput screening of protein-protein interactions in yeast liquid culture. Protein fusions non-native to yeast may be expressed to replace endogenous sexual agglutination proteins and mediate library-by-library interrogation of protein interactions. The methods and compositions of the invention can be utilized for the characterization of protein interaction networks in high throughput for both binding affinity and specificity, which is crucial for understanding cellular functions, screening therapeutic candidates, and evaluating engineered protein networks.
    Type: Grant
    Filed: April 23, 2020
    Date of Patent: April 27, 2021
    Assignee: UNIVERSITY OF WASHINGTON
    Inventors: David Baker, David Younger, Eric Klavins
  • Patent number: RE48561
    Abstract: A system and method for imaging biological samples on multiple surfaces of a support structure are disclosed. The support structure may be a flow cell through which a reagent fluid is allowed to flow and interact with the biological samples. Excitation radiation from at least one radiation source may be used to excite the biological samples on multiple surfaces. In this manner, fluorescent emission radiation may be generated from the biological samples and subsequently captured and detected by detection optics and at least one detector. The detected fluorescent emission radiation may then be used to generate image data. This imaging of multiple surfaces may be accomplished either sequentially or simultaneously. In addition, the techniques of the present invention may be used with any type of imaging system. For instance, both epifluorescent and total internal reflection methods may benefit from the techniques of the present invention.
    Type: Grant
    Filed: June 5, 2018
    Date of Patent: May 18, 2021
    Assignee: ILLUMINA, INC.
    Inventors: Wenyi Feng, Jason Bryant, Steven Barnard, Maria Candelaria Rogert Bacigalupo
  • Patent number: RE48608
    Abstract: The present invention provides methods to determine whether a patient with a lysosomal storage disorder will benefit from treatment with a specific pharmacological chaperone. The present invention exemplifies an in vitro method for determining ?-galactosidase A responsiveness to a pharmacological chaperone such as 1-deoxygalactonojirimycin in a cell line expressing a mutant from of ?-galactosidase A. The invention also provides a method for diagnosing Fabry disease in patients suspected of having Fabry disease.
    Type: Grant
    Filed: December 17, 2018
    Date of Patent: June 29, 2021
    Assignee: Amicus Therapeutics, Inc.
    Inventors: Elfrida Benjamin, Hung V. Do, John Flanagan, Xiaoyang Wu, Brandon Wustman