Measuring Or Testing Process Involving Enzymes Or Micro-organisms; Composition Or Test Strip Therefore; Processes Of Forming Such Composition Or Test Strip Patents (Class 435/4)
  • Patent number: 10660880
    Abstract: The present invention relates to a dosing regimen for a selective S1P1 receptor agonist, whereby the selective S1P1 receptor agonist is administered to a subject in such a way that during the initial treatment phase the selective S1P1 receptor agonist is administered at a dose which induces desensitization of the heart wherein said dose is below the target dose, and at a dosing frequency that sustains desensitization of the heart, until no further acute heart rate reduction occurs, followed by dose up-titration to the target dose of the selective S1P1 receptor agonist.
    Type: Grant
    Filed: July 2, 2014
    Date of Patent: May 26, 2020
    Assignee: ACTELION PHARMACEUTICALS LTD
    Inventors: Patrick Brossard, Jasper Dingemanse, Oliver Nayler, Michael Scherz, Beat Steiner
  • Patent number: 10654232
    Abstract: The present invention relates to a method for manufacturing an optical element (100) having at least one functional region using a 3D-printer, comprising the steps: forming a three-dimensional structure (50) of the optical element (100) using a 3D-printer such that the three-dimensional structure (100) has at least one microfluidic cavity (4) for receiving a functional substance (6); and filling the at least one microfluidic cavity (4) with the functional substance (6) for forming the at least one functional region. In addition, the invention relates to a device for manufacturing an optical element (100) as well as a use of the device.
    Type: Grant
    Filed: October 27, 2016
    Date of Patent: May 19, 2020
    Assignee: BADEN-WUERTTEMBERG STIFTUNG GGMBH
    Inventors: Simon Thiele, Harald Giessen, Timo Gissibl, Alois M. Herkommer
  • Patent number: 10646600
    Abstract: A method of use of an isolated polypeptide conjugated with a radionuclide, wherein the isolated polypeptide binds specifically to HER2, or a variant thereof is described. The method is for monitoring the response to HSP90 inhibition and comprises the use of the isolated polypeptide conjugated with 18F. The application also describes the use of an isolated polypeptide conjugated with 18F, wherein the isolated polypeptide binds specifically to HER2 or variants thereof, as an imaging agent to monitor uptake thereof to measure HSP90 inhibition.
    Type: Grant
    Filed: June 24, 2014
    Date of Patent: May 12, 2020
    Assignees: IMPERIAL INNOVATIONS LIMITED, GE HEALTHCARE LIMITED
    Inventors: Duncan Robert R. Hiscock, Eric Aboagye, Peter Iveson, Quang-De Nguyen, Susan Hoppmann, Sebastian Trousil, Maciej Kaliszczak, Giampaolo Tomasi
  • Patent number: 10647953
    Abstract: An article suitable for inducing quasispherical cell clustering is provided. The article comprising: a layer suitable for culturing quasispherical cell clusters, the layer comprising a bulk region comprising an interpenetrating network of at least one predominantly positively charged polyelectrolyte and at least one predominantly negatively charged positive polyelectrolyte, a back surface region, and a front surface region, wherein the front surface region comprises a net negative fixed surface charge density of between about 0.4 micromole per m2 and about 1.5 micromole per m2.
    Type: Grant
    Filed: January 25, 2016
    Date of Patent: May 12, 2020
    Assignee: The Florida State University Research Foundation, Inc.
    Inventors: Joseph B. Schlenoff, Carlos Arias
  • Patent number: 10640822
    Abstract: The disclosure provides a novel system of storing information using a charged polymer, e.g., DNA, the monomers of which correspond to a machine-readable code, e.g., a binary code, and which can be synthesized and/or read using a novel nanochip device comprising nanopores; novel methods and devices for synthesizing oligonucleotides in a nanochip format; novel methods for synthesizing DNA in the 3? to 5? direction using topoisomerase; novel methods and devices for reading the sequence of a charged polymer, e.g., DNA, by measuring capacitive or impedance variance, e.g., via a change in a resonant frequency response, as the polymer passes through the nanopore; and further provides compounds, compositions, methods and devices useful therein.
    Type: Grant
    Filed: May 2, 2018
    Date of Patent: May 5, 2020
    Assignee: IRIDIA, INC.
    Inventors: Paul F. Predki, Maja Cassidy
  • Patent number: 10633702
    Abstract: Aspects of the present invention include analyzing nucleic acids from single cells using methods that include using tagged polynucleotides containing multiplex identifier sequences.
    Type: Grant
    Filed: June 17, 2019
    Date of Patent: April 28, 2020
    Assignee: 10X GENOMICS, INC.
    Inventors: Sydney Brenner, Gi Mikawa, Robert Osborne, Andrew Slatter
  • Patent number: 10626158
    Abstract: The present invention discloses cryopreserved recombinant cells for screening drug candidates that transiently overexpress one or more drug transporter proteins and/or drug metabolizing enzymes. Advantageously, such cells provide a cost-efficient consumable product that streamlines the process of screening whether drug candidates are substrates or inhibitors of drug transporter proteins and/or drug metabolizing enzymes.
    Type: Grant
    Filed: May 9, 2019
    Date of Patent: April 21, 2020
    Assignee: Corning Incorporated
    Inventors: Na Li, Jie Wang, Christopher J. Patten
  • Patent number: 10624829
    Abstract: The purpose of this invention is to find a novel fragrance component effective for prevention or control of swelling, lymphedema, skin aging or obesity from the viewpoint of activation of lymph vessel function by promotion of the expression of VEGFC. This invention provides a swelling improving agent, lymph vessel activator and VEGFC production promoter characterized by containing one or a plurality of components selected from the group consisting of rose oxide, citronellyl acetate and niaouli oil.
    Type: Grant
    Filed: June 16, 2017
    Date of Patent: April 21, 2020
    Assignee: Shiseido Company, Ltd.
    Inventors: Mio Nakamura, Kentaro Kajiya, Shinichiro Haze
  • Patent number: 10627421
    Abstract: An apparatus for biological reactions is provided. The apparatus includes a substrate and a plurality of reaction sites within the substrate. A surface of the substrate is configured to have a first hydrophilicity and each surface of the plurality of reaction sites is configured to have a second hydrophilicity to load a substantial number of reaction sites with a sample volume. The sample volume of each loaded reaction site is substantially confined to its respective reaction site. The sample volume is configured to undergo a biological reaction within the reaction site.
    Type: Grant
    Filed: March 15, 2013
    Date of Patent: April 21, 2020
    Assignee: Life Technologies Corporation
    Inventors: Eliodor Ghenciu, Theodore E. Straub, Evan W. Foster, Michael C. Pallas
  • Patent number: 10610863
    Abstract: An automatic microfluidic system for antibiotic susceptibility testing of the present disclosure at least includes a microfluidic chip. The microfluidic chip includes a fluid storage unit, a reaction unit, a pneumatic micro-pumping unit and a plurality of valve units. The fluid storage unit is provided for storing a bacterial suspension, a broth and an antibiotic solution. The reaction unit includes a first reaction chamber and at least two second reaction chambers. The pneumatic micro-pumping unit is adjacently disposed to the fluid storage unit and the reaction unit for selectively, repeatedly and quantitatively transporting the broth, the bacterial suspension and the antibiotic solution to the reaction unit to form a first mixing solution and at least two second mixing solutions. The valve units include a plurality of pneumatic micro-valves and a plurality of valve control air holes for controlling the opening and closing of the pneumatic micro-valves.
    Type: Grant
    Filed: May 1, 2017
    Date of Patent: April 7, 2020
    Assignees: NATIONAL TSING HUA UNIVERSITY, Kaohsiung Chang Gung Memorial Hospital
    Inventors: Gwo-Bin Lee, Shiuann-Sheng Lee, Wen-Bin Lee, Wen-Hsin Chang
  • Patent number: 10590408
    Abstract: The present invention relates to biomolecule stabilization to provide biomolecules, such as sensitive polymerases, in a convenient ready-to-go format. The invention provides a method and composition in which non-ionic surfactant or detergents of the polyoxyethylene cetyl ether family are used, preferably a Brij reagent or a combination of Brij reagents.
    Type: Grant
    Filed: February 10, 2017
    Date of Patent: March 17, 2020
    Assignee: GE Healthcare UK Limited
    Inventors: Peter James Tatnell, Elizabeth Mary Ashman
  • Patent number: 10578626
    Abstract: Disclosed are a kit for testing myocardial infarction rapidly and a preparation method and use thereof. The kit comprises a strip capable of detecting three markers, namely, human myeloperoxidase (MPO), heart-fatty acid binding protein (FABP3) and cardiac troponin I (cTnI) simultaneously. The strip comprises a sample pad, a conjugate pad, a chromatographic membrane coated with three test lines and a quality control line, and a sample absorption pad. Antibodies of the three markers are all marked on the conjugate pad. The chromatographic membrane has three test lines formed by coating paired antibodies of the three markers respectively, the paired antibodies of the three markers being able to specifically combine with the three markers, respectively. The kit has advantages such as convenient operation, rapid response, high sensitivity and high specificity, point of care test, and economical and practical etc.
    Type: Grant
    Filed: April 15, 2015
    Date of Patent: March 3, 2020
    Assignees: EACHY BIOPHARMACEUTICALS CO., LTD.
    Inventors: Wenbo Hu, Jing Li
  • Patent number: 10564074
    Abstract: Rose Bengal for detecting a presence of chemical warfare agents. A method of detecting presence of a chemical warfare agent and includes applying a quinoid form of Rose Bengal to a substrate. When the substrate is exposed to the chemical warfare agent, a lactone form of Rose Bengal is spectrally observed because presence of the chemical warfare agent converts the quinoid form to the lactone form of Rose Bengal.
    Type: Grant
    Filed: March 2, 2016
    Date of Patent: February 18, 2020
    Assignee: United States of America as represented by the Secretary of the Air Force
    Inventors: Jeffery Ray Owens, Rashelle S. McDonald, Dmytro Volkov
  • Patent number: 10551372
    Abstract: Methods of characterizing a test subject's risk of having or developing cardiovascular disease are provided. The methods include using an analytic device to determine levels of choline-related trimethylamine-containing compounds such as trimethylamine N-oxide, choline, or betaine in a biological sample obtained from the subject and comparing the levels of the choline-related trimethylamine-containing compound in the subject's biological sample to a control value. The test subject's risk of having cardiovascular disease is then characterized as higher if the levels of the choline-related trimethylamine-containing compound are higher than the control value. Also provided are methods of identifying a subject at risk of experiencing a complication of atherosclerotic cardiovascular disease, and methods of evaluating the efficacy of a cardiovascular therapeutic agent in a subject with cardiovascular disease using levels of choline-related trimethylamine-containing compounds.
    Type: Grant
    Filed: August 11, 2016
    Date of Patent: February 4, 2020
    Assignee: The Cleveland Clinic Foundation
    Inventors: Stanley L. Hazen, Zeneng Wang, Bruce S. Levison
  • Patent number: 10540535
    Abstract: Techniques for identifying a region of interest in an image of a biological cell include detecting a primary axis of a structure of the biological cell and defining a first section line along the detected primary axis, detecting at least one secondary axis of the structure of the biological cell and defining a second section line along each of the at least one secondary axes. Based on the second section line along each of the at least one secondary axes and the first section line along the detected primary axis, a cytoplasm of the cell is divided into a plurality of areas. A geometric feature of each of the areas is determined and one of the areas is selected according to predefined criteria. The image data of the selected area is analyzed, and the image data is compared with the predefined criteria to identify regions that comply with the provided criteria. At least one area complying with the predefined criteria to a pre-defined degree is selected as the region of interest.
    Type: Grant
    Filed: March 12, 2018
    Date of Patent: January 21, 2020
    Assignee: CARL ZEISS MICROSCOPY GMBH
    Inventor: Jeffrey Scott McDonald
  • Patent number: 10529454
    Abstract: Systems and methods for extracting blood glucose patterns and suggesting a behavior may include receiving, at a computing device comprising a processor, temporal data including information regarding glucose readings; identifying, by the computing device, at least one pattern based on metabolite levels extracted from the temporal data the model including variables corresponding to each of the patterns; formulating, by the computing device, a model for predicting a metabolic response; and storing the model on a data storage device. Based on the model, the behavior may be suggested to maintain a blood glucose level within a desired range.
    Type: Grant
    Filed: October 15, 2015
    Date of Patent: January 7, 2020
    Inventor: Bradley E. Kahlbaugh
  • Patent number: 10520488
    Abstract: A method for determination of the growth rate of biofilm using an electrical impedance analyses is disclosed. The method comprises the steps of: bringing a culture medium fluid in contact to an electrode structure, having biofilm grown within the fluid culture medium with the biofilm arranged in distance to the electrodes structure, so that the fluid culture medium is placed between the growing biofilm and the electrode structure; measuring the impedance of the electrodes structure over a monitoring time, and determining the growth rate of the biofilm as a function of the reduction rate of the impedance values measured on the electrode structure.
    Type: Grant
    Filed: December 18, 2015
    Date of Patent: December 31, 2019
    Assignee: Helmholtz-Zentrum fuer Infektionsforschung GmbH
    Inventors: Jost Van Duuren, Blanka Karge, Christoph Wittmann, Mark Broenstrup
  • Patent number: 10519492
    Abstract: A disposable assay platform for detecting a target nucleic acid comprising multiple chambers and a method for operating the assay platform. Solutions containing the target nucleic acid move from one chamber to the next chamber by opening a vent pocket. The resulting pressure change enables the solution to flow to the next chamber. The platform comprises an electronic layer and one or more fluid layers bonded together. All heating operations can be performed by using resistive heating elements in the platform. All cooling operations are preferably passive. The platform is preferably operated when in a vertical orientation and can be docked to an external docking station that controls the operation of the platform.
    Type: Grant
    Filed: April 20, 2012
    Date of Patent: December 31, 2019
    Assignee: Mesa Biotech, Inc.
    Inventors: Marc DeJohn, Robert B. Cary, Nathan J. Cobb
  • Patent number: 10509034
    Abstract: Disclosed are bladder cancer protein biomarkers, methods of determining whether a patient suffers from or shows recurrence of bladder cancer or early stage bladder cancer, or late stage bladder cancer using the bladder cancer protein biomarkers, a detection system, and kits thereof. Said bladder cancer biomarkers comprise at least one of Coronin-IA, Apolipoprotein A-IV, Semenogelin-2, Gamma-synuclein and DJ-1, and variants thereof.
    Type: Grant
    Filed: November 5, 2014
    Date of Patent: December 17, 2019
    Assignee: Agency for Science, Technology and Research
    Inventors: Jean Paul Thiery, Prashant Kumar, Jayantha Gunaratne
  • Patent number: 10478819
    Abstract: A microfluidic device includes a particle sorting region having a first, second and third microfluidic channels, a first array of islands separating the first microfluidic channel from the second microfluidic channel, and a second array of islands separating the first microfluidic channel from the third microfluidic channel, in which the island arrays and the microfluidic channels are arranged so that a first fluid is extracted from the first microfluidic channel into the second microfluidic channel and a second fluid is extracted from the third microfluidic channel into the first microfluidic channel, and so that particles are transferred from the first fluid sample into the second fluid sample within the first microfluidic channel.
    Type: Grant
    Filed: August 24, 2018
    Date of Patent: November 19, 2019
    Assignee: The General Hospital Corporation
    Inventors: Ravi Kapur, Kyle C. Smith, Mehmet Toner
  • Patent number: 10481148
    Abstract: Provided are a cell line stably expressing voltage-gated sodium channel (Nav1.5), wherein a SCN5A gene (NCBI accession no. NM_198056) encoding Nav1.5 and a puromycin selection marker are inserted into a chromosome of a host cell, and a method of measuring cardiotoxicity of a test substance comprising: (1) contacting a test substance to the cell line of claim 1; and (2) measuring magnitude of voltage-gated sodium channel (Nav1.5) in the cell line in contact with the test substance.
    Type: Grant
    Filed: February 28, 2017
    Date of Patent: November 19, 2019
    Assignee: THE CATHOLIC UNIVERSITY OF KOREA INDUSTRY-ACADEMIC COOPERATION FOUNDATION
    Inventors: Jin-Sung Choi, Jeong Hee Choi
  • Patent number: 10482655
    Abstract: An approach is provided for providing a digital elevation model. For example, the approach involves processing, by a processor, map elevation data to create a hierarchical resolution tile representation of the digital elevation model for a geographic area. The hierarchical resolution tile representation includes a plurality of resolution levels, wherein each of the plurality of levels represents the digital elevation model at a different resolution. The digital elevation model includes a plurality of control points, wherein each of the plurality of control points is associated with an elevation data point determined from the map elevation data.
    Type: Grant
    Filed: November 20, 2017
    Date of Patent: November 19, 2019
    Assignee: HERE Global B.V.
    Inventors: Xin Chen, Andi Zang
  • Patent number: 10470445
    Abstract: Described herein are rats with a hepatic deficiency comprising decreased function, activity, or expression of an enzyme in the tyrosine catabolic pathway (such as fumarylacetoacetate hydrolase), and methods of using the same for in vivo engraftment and expansion of heterologous hepatocytes, such as human hepatocytes, analysis of human liver disease, and analysis of xenobiotics. Also disclosed is the use of immunodeficient rats for the engraftment and expansion of heterologous hepatocytes.
    Type: Grant
    Filed: May 19, 2016
    Date of Patent: November 12, 2019
    Assignee: Yecuris Corporation
    Inventors: John R. Bial, Elizabeth M. Wilson, Aron M. Geurts
  • Patent number: 10451641
    Abstract: Disclosed herein are compositions and methods for targeted metabolomics profiling to provide absolute quantification of a broad array of metabolic intermediates of fatty acids, amino acids, and carbohydrate metabolism for HFpEF. In an aspect, the disclosure relates to a method for assessing risk of death or a major adverse cardiac event (MACE) in a subject diagnosed with heart failure with preserved ejection fraction (HFpEF). The method may include (a) determining in a sample from the subject the level of each metabolite in at least one factor, wherein the factor is selected from the group consisting of factor 1, factor 3, factor 4, factor 5, factor 10, factor 12, and factor 14; and (b) comparing the level of each metabolite in the sample to a standard, wherein the level of the metabolite in the subject relative to a standard is indicative of the risk of death or MACE in the subject.
    Type: Grant
    Filed: March 14, 2016
    Date of Patent: October 22, 2019
    Assignee: Duke University
    Inventor: Svati Shah
  • Patent number: 10449542
    Abstract: Methods and devices for detecting metabolic activity of target cells in a sample. The target cells are concentrated in a nanoliter well having a microfilter. A reporter compound that exhibits a change in electrochemical state in response to metabolic activity of the target cells is introduced. Metabolic activity or viability of the target cells is detected based on a determined change in the electrochemical state of contents in the well.
    Type: Grant
    Filed: October 28, 2015
    Date of Patent: October 22, 2019
    Assignee: The Governing Council of the University of Toronto
    Inventors: Shana Olwyn Kelley, Edward Hartley Sargent, Justin David Besant
  • Patent number: 10444229
    Abstract: This invention provides a method for measuring insulin resistance of a test subject, and a composition suitably used for the method. A method for measuring insulin resistance of a test act comprising steps (a) and (b) below, the method using a composition for measuring insulin resistance comprising, as an active ingredient, a C12-38 fatty acid labeled with at least one isotope of C, wherein the C12-38 fatty acid is converted in the body into labeled carbon dioxide that is excreted in expired air, (a) intravenously administering the composition to a test subject and collecting expired air; and (b) determining the ratio of labeled CO2 amount to unlabeled CO2 amount contained in the expired air or the ratio of labeled CO2 amount to total CO2 amount contained in the expired air.
    Type: Grant
    Filed: March 13, 2014
    Date of Patent: October 15, 2019
    Assignee: Otsuka Pharmaceutical Co., Ltd.
    Inventors: Makoto Inada, Jun-ichi Kunizaki, Kazuki Tobita, Hiroshi Motonari, Tetsuya Sato
  • Patent number: 10443104
    Abstract: It has been discovered that the human GT198 gene (gene symbol PSMC3IP) at chromosome 17q21 acts as a tumor suppressor. The mutation of the GT198 gene causes the increased dominant negative splice variant activity and leads to the loss of wild type GT198 function, and in turn, induces breast and ovarian cancers. One embodiment provides compositions and methods for treating or alleviating one or more symptoms associated with cancer due to the GT198 gene mutations. Another embodiment provides methods and compositions for detecting cancer due to the mutation of the GT198 gene. Still another embodiment provides methods for identifying compounds, antibodies and natural product molecules that are useful for treating cancer due to the mutations of the GT198 gene. Preferably the disclosed compositions antagonize or interfere with the biological activity of splice variants of GT198.
    Type: Grant
    Filed: June 2, 2017
    Date of Patent: October 15, 2019
    Inventor: Lan Ko
  • Patent number: 10443092
    Abstract: The present invention relates to methods of elongating chromosomes. Embodiments of the present disclosure are directed to methods of elongating DNA by immobilizing or attaching the DNA to a substrate. According to one aspect, naturally occurring DNA includes a nucleic acid and one or more factors bound thereto, and may be referred to herein as “starting DNA”.
    Type: Grant
    Filed: March 11, 2014
    Date of Patent: October 15, 2019
    Assignee: President and Fellows of Harvard College
    Inventor: Chao-ting Wu
  • Patent number: 10435480
    Abstract: The present invention provides a method for the functionalization of a polymeric surface with a protein by physical adsorption. The method enables a membrane spanning protein to be used as an anchor for proteins and/or peptides for display. Also provided are polymeric substrates for protein or peptide display, and related kits and methods of use.
    Type: Grant
    Filed: January 14, 2015
    Date of Patent: October 8, 2019
    Assignee: Porvair Sciences Limited
    Inventors: Deepan Shah, Sion Phillips
  • Patent number: 10429393
    Abstract: The present invention provides a method for concurrent resolution of the cancer stem cell (CSC) derived hierarchy, genetic instability, differentially cycling cells and host cells recruited for performing tumor growth supporting functions; and (ii) quantification, monitoring and analysis of these populations. The first level of analysis can be carried out using either CSC- and progenitor-specific markers or a marker-free approach based on label-chase to resolve the tumor regenerative hierarchy. The next level involves combinatorial quantification of differential DNA-RNA contents to identify recruited host and tumor cell variants resulted from genetic instability and differential cycling within the tumor.
    Type: Grant
    Filed: January 17, 2015
    Date of Patent: October 1, 2019
    Assignee: NATIONAL CENTRE FOR CELL SCIENCE
    Inventors: Sharmila A. Bapat, Rutika R. Naik
  • Patent number: 10422760
    Abstract: Object information representing a honeycomb structure with a plurality of meshes is obtained, and an inner-wall-surface heat transfer coefficient hs, i.e., a heat transfer coefficient between an inner wall surface of a cell and a fluid, is derived as follows. First, one of the meshes as a target for derivation of the inner-wall-surface heat transfer coefficient hs is set (S200), and a dimensionless coordinate X* is derived on the basis of position information (X-coordinate) of the set mesh and fluid state information (S210). An inner-wall-surface dimensionless heat transfer coefficient Nus corresponding to the derived dimensionless coordinate X* is then derived on the basis of the inner-wall-surface dimensionless correspondence information (S220 to S250). The inner-wall-surface heat transfer coefficient hs in the mesh set as the derivation target is then derived on the basis of the derived inner-wall-surface dimensionless heat transfer coefficient Nus (S260).
    Type: Grant
    Filed: March 28, 2016
    Date of Patent: September 24, 2019
    Assignee: NGK Insulators, Ltd.
    Inventors: Satoshi Sakashita, Shingo Sokawa, Rishun Kin, Norihisa Fujie
  • Patent number: 10408843
    Abstract: The present invention relates to methods for detecting protein S-sulfinylation and S-sulfinylation within thiol groups in proteins, metabolites, or materials.
    Type: Grant
    Filed: February 17, 2017
    Date of Patent: September 10, 2019
    Assignee: The Regents of the University of Michigan
    Inventors: Brent R. Martin, Jaimeen D. Majmudar
  • Patent number: 10408848
    Abstract: A three dimensional (3-D) model comprising a scaffold and autologous skin cells, the invention also provides methods of predicting immunogenicity and hypersensitivity or allergic or adverse immune reactions to potential therapeutic compounds, biologies, cosmetics and chemical sensitizers using the 3-D model of skin cells. The methods provide an in vitro assay employing autologous blood derived cells in the 3-D skin equivalent model and is of particular utility in the identification and prediction of skin sensitizers and in particular agents that may cause allergic contact dermatitis. The assay of the present invention provides inter alia methods of screening library compounds for sensitizing activity, identifying optimal therapeutics, especially but not exclusively, monoclonal antibodies and kits therefor.
    Type: Grant
    Filed: February 27, 2014
    Date of Patent: September 10, 2019
    Assignees: Alcyomics LTD, University of Newcastle Upon Tyne
    Inventors: Penny Lovat, David Hill, Anne Dickinson, Shaheda Ahmed
  • Patent number: 10400270
    Abstract: A technique that uses nanotechnology to electrically detect and identify RNA sequences without the need for using enzymatic amplification methods or fluorescent labels. The technique may be scaled into large multiplexed arrays for high-throughput and rapid screening. The technique is further able to differentiate closely related variants of a given bacterial or viral species or strain. This technique addresses the need for a quick, efficient, and inexpensive bacterial and viral detection and identification system.
    Type: Grant
    Filed: September 12, 2017
    Date of Patent: September 3, 2019
    Assignee: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
    Inventors: Andre Knoesen, Paul Alexander Feldstein, Joshua Hihath, Erkin Seker, Maria Louise Marco, Bryce William Falk
  • Patent number: 10391067
    Abstract: The pharmacokinetics and key technologies of the present invention are summarized in FIG. 1. Particularly, malignant misfolded proteins such as mutant huntingtin and alpha-synuclein are coagulated and grow into oligomeric coagulum ({circle around (1)}, {circle around (2)}, fibrillar coagulum ({circle around (3)}) and eventually inclusion body ({circle around (4)}). Young neurons produce a large amount of Nt-Arg through N-terminal arginylation ({circle around (5)}) of vesicle chaperones such as BiP secreted into the cytoplasm, and then arginylated BiP (R-BiP) is secreted binds to the misfolded proteins ({circle around (6)}). As a ligand, the Nt-Arg of R-BiP binds to the p62 ZZ domain ({circle around (7)}), and the normally inactivated closed form of p62 is changed to an open form, leading to structural activation ({circle around (8)}). As a result, PB1 and LC3-binding domains are exposed.
    Type: Grant
    Filed: July 15, 2016
    Date of Patent: August 27, 2019
    Assignee: AUTOTAC BIO
    Inventors: Yong Tae Kwon, Bo Yeon Kim, Hyunjoo Cha, Young Dong Yoo, Ji-eun Yu
  • Patent number: 10392610
    Abstract: The present invention provides pregnancy associated plasma protein A2 (PAPP-A2), its nucleotide and amino acid sequences, antisense molecules to the nucleotide sequences which encode PAPP-A2, expression vectors for the production of purified PAPP-A2, antibodies capable of binding specifically to PAPP-A2, hybridization probes or oligonucleotides for the detection of PAPP-A2-encoding nucleotide sequences, genetically engineered host cells for the expression of PAPP-A2, and methods for screening for pathologies in pregnant and non-pregnant patients. Methods for screening for altered focal proliferation states in pregnant and/or non-pregnant patients, which include detecting levels of PAPP-A2, are also described.
    Type: Grant
    Filed: August 22, 2014
    Date of Patent: August 27, 2019
    Assignee: Como Biotech ApS
    Inventors: Claus Oxvig, Michael Toft Overgaard
  • Patent number: 10391485
    Abstract: A microfluidic device useable for performing live cell computed tomography imaging is fabricated with a cover portion including a first wafer with at least one metal patterned thereon, a base portion including a second wafer with at least one metal patterned thereon and negative photoresist defining recesses therein, and a diffusive bonding layer including a negative photoresist arranged to join the cover portion and the base portion. A composition useful in live cell computer topography includes a long-chain polysaccharide at a concentration of from about 0.01% to about 10.0% in cell culture medium for supporting cell life while enabling cell rotation rate to be slowed to a speed commensurate with low light level imaging.
    Type: Grant
    Filed: September 25, 2014
    Date of Patent: August 27, 2019
    Assignee: ARIZONA BOARD OF REGENTS, A BODY CORPORATE OF THE STATE OF ARIZONA, ACTING FOR AND ON BEHALF OF ARIZONA STATE UNIVERSITY
    Inventors: Deirdre Meldrum, Roger Johnson, Iniyan Soundappa Elango, Andrew Shabilla, Hong Wang, Jakrey Myers, Laimonas Kelbauskas, Dean Smith, Pimwadee Limsirichai
  • Patent number: 10386371
    Abstract: Provided herein are methods for measuring molecular flux rates of molecules of interest in a tissue sample in spatially-organized manner and generating output (e.g., an image, a heat map, a contour map, a table or a database) representing the molecular flux rates of each spatially-defined location of the sample. Provided herein are also the output, as well as systems and computer-readable medium with computer-executable instructions for determining molecular flux rates of molecules of interest in the sample.
    Type: Grant
    Filed: September 7, 2012
    Date of Patent: August 20, 2019
    Assignee: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
    Inventors: Benjamin P. Bowen, Katherine B. Louie, Trent R. Northen, Marc K. Hellerstein
  • Patent number: 10386327
    Abstract: A bioFET cell for measuring a time dependent characteristic of an analyte bearing fluid includes a source, a drain, a semiconductive single wall carbon nanotube network layer extending between the source and drain electrodes and electrically coupled there between, a gate insulatively spaced from and disposed over and extending between the source and drain electrodes, a layer of at least one selected antibody disposed on and linked to the polymer layer to functionalize the semiconductive single wall carbon nanotube network layer to a selected target biomarker corresponding to the at least one selected antibody so that electron transport into the semiconductive single wall carbon nanotube network layer is facilitated, where the source, drain and gate electrodes with the carbon nanotube network layer form a defined channel through which the analyte bearing fluid may flow, and a high impedance source follower amplifier coupled to the source electrode.
    Type: Grant
    Filed: October 4, 2017
    Date of Patent: August 20, 2019
    Assignee: Sensor Kinesis Corporation
    Inventor: Josh Shachar
  • Patent number: 10357217
    Abstract: A method of using PET imaging includes using PET images obtained using a radiotracer for the determination of the progressive course of regional brain PET activities for a progressive neurodegenerative disease (e.g., MCI, AD, and CTE). The method may be used for the automatic staging of neurodegenerative disease for a particular patient/subject based on regional intensity and spatial patterns of the brain signals measured by PET imaging. The method may also be used to diagnose or classify the disease of a patient among multiple possibilities based on PET imaging profiles (e.g., for separating or distinguishing CTE from AD).
    Type: Grant
    Filed: October 18, 2017
    Date of Patent: July 23, 2019
    Assignee: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
    Inventors: Sung-Cheng Huang, Koon-Pong Wong, Gary W. Small, Jorge R. Barrio
  • Patent number: 10350320
    Abstract: Nanoparticles as described herein are configured to bind to bacterial contaminants, such as Gram positive bacteria, Gram negative bacteria, and endotoxins. The nanoparticles include a core comprising a magnetic material; and a plurality of ligands attached to the core. The ligands include, for example, bis(dipicolylamine) (“DPA”) coordinated with a metal ion, e.g., Zn2+ or Cu2+, to form, e.g., bis-Zn-DPA or bis-Cu-DPA, which can bind to the bacterial contaminants. The nanoparticles can be included in compositions for use in methods and systems to separate bacterial contaminants from liquids, such as liquids, such as blood, e.g., whole or diluted blood, buffer solutions, albumin solutions, beverages for human and/or animal consumption, e.g., drinking water, liquid medications for humans and/or animals, or other liquids.
    Type: Grant
    Filed: January 29, 2014
    Date of Patent: July 16, 2019
    Assignees: Children's Medical Center Corporation, Massachusetts Institute of Technology
    Inventors: Jung-Jae Lee, Kyung Jae Jeong, Daniel S. Kohane
  • Patent number: 10352942
    Abstract: Described herein are methods for identifying signature peptides for quantifying a polypeptide of interest in a sample. The methods include cleaving the polypeptide into peptides; detecting a multiplicity of the peptides with a quantitative analytical instrument; comparing the linearity of signals attributable to pairs of the peptides in a multiplicity of samples; and selecting signature peptides from a group of peptides with more highly correlated signals.
    Type: Grant
    Filed: May 31, 2016
    Date of Patent: July 16, 2019
    Assignees: Cedars-Sinai Medical Center, The Johns Hopkins University
    Inventors: Eric Grote, Qin Fu, Jennifer Van Eyk, Vidya Venkatraman
  • Patent number: 10338080
    Abstract: The invention provides methods for sensitive and specific detection of complement fixing antibodies in a biological sample using complement factor C1q, including autologous complement factor C1q present in the biological sample and a detectably labeled antibody that binds the autologous complement factor C1q, exogenous human complement factor C1q and a detectably labeled antibody that binds the exogenous human complement factor C1q, detectably labeled exogenous human complement factor C1q, or a combination of autologous complement factor C1q and exogenous human complement factor C1q. The invention also features kits, systems, and devices for use in the methods of the invention.
    Type: Grant
    Filed: November 25, 2009
    Date of Patent: July 2, 2019
    Assignee: The Board of Trustees of the Leland Stanford Junior University
    Inventors: Dolly B. Tyan, Ge Chen
  • Patent number: 10328040
    Abstract: The invention provides a method for a disease or condition associated with dopamine deficiency (e.g. depression, schizophrenia, or Parkinson's disease) in a mammal in need of such treatment comprising administering a compound that binds to RXR to the mammal.
    Type: Grant
    Filed: January 20, 2015
    Date of Patent: June 25, 2019
    Assignee: ARIZONA BOARD OF REGENTS ON BEHALF OF ARIZONA STATE UNIVERSITY
    Inventors: Pamela Marshall, Peter Jurutka, Carl Wagner
  • Patent number: 10329626
    Abstract: The present invention features a method for determining the prognosis for survival of a cancer patient. Methods for measuring the level of NOL3 expression in a cancer cell-containing sample from a patient, and comparing the level of NOL3 expression in the sample to a reference level of NOL3 expression are also included. A higher level of NOL3 relative to the reference level correlates with decreased survival of the patient, and an equivalent or lower level of NOL3 relative to the reference level correlates with increased survival of the patient.
    Type: Grant
    Filed: December 8, 2016
    Date of Patent: June 25, 2019
    Assignee: Dana-Farber Cancer Institute, Inc.
    Inventors: Andrew Kung, David Ziegler
  • Patent number: 10322216
    Abstract: The present invention relates to a filtering device (8) for removing substances from blood or a blood component, the filtering device (8) comprising: a housing having an inlet and an outlet, at first sorbent material coupled with at least a first ligand located within the housing, and a second sorbent material coupled with at least a second ligand located within the housing, wherein the first ligand is for removing free hemoglobin (fHb) and the second ligand is for removing microvesicles (MV) from the blood or blood component passing through the filtering device (8), from the inlet to the outlet, wherein the first and second ligand are different from each other, and wherein the first and second sorbent material are the same or are different from each other.
    Type: Grant
    Filed: December 17, 2014
    Date of Patent: June 18, 2019
    Assignee: Fresenius Hemocare Italia S.R.L.
    Inventors: Giorgio Mari, Serena Borghi, Elena Finetti
  • Patent number: 10324059
    Abstract: A bioFET cell for measuring a time dependent characteristic of an analyte bearing fluid includes a source, a drain, a semiconductive single wall carbon nanotube network layer extending between the source and drain electrodes and electrically coupled there between, a gate insulatively spaced from and disposed over and extending between the source and drain electrodes, a layer of at least one selected antibody disposed on and linked to the polymer layer to functionalize the semiconductive single wall carbon nanotube network layer to a selected target biomarker corresponding to the at least one selected antibody so that electron transport into the semiconductive single wall carbon nanotube network layer is facilitated, where the source, drain and gate electrodes with the carbon nanotube network layer form a defined channel through which the analyte bearing fluid may flow, and a high impedance source follower amplifier coupled to the source electrode.
    Type: Grant
    Filed: October 4, 2017
    Date of Patent: June 18, 2019
    Assignee: Sensor-Kinesis Corporation
    Inventor: Josh Shachar
  • Patent number: 10311582
    Abstract: The present disclosure relates to a data processing apparatus and a data processing method capable of generating evaluation index data for performing accurate and detailed evaluation of cultured cardiomyocytes. A motion detecting unit divides frame image data obtained by photographing the cultured cardiomyocytes for a predetermined time into blocks and obtains motion detection data in units of blocks per each frame period. A feature amount calculating unit calculates a feature amount for each block at the same position in a frame image using the motion detection data. A classification processing unit classifies each of the blocks into any one of a plurality of classification categories using the calculated feature amount. On the basis of the classification result, evaluation index data made of individual classification result data that represent correspondences between the blocks and the classification categories is generated.
    Type: Grant
    Filed: August 4, 2017
    Date of Patent: June 4, 2019
    Assignee: Sony Corporation
    Inventors: Takeshi Kunihiro, Masashi Uchida, Eriko Matsui, Tomohiro Hayakawa
  • Patent number: 10292988
    Abstract: This invention discloses uses for estrone in preparing anti-ovarian cancer and/or breast cancer products. This invention provides uses for estrone in the preparation of products to treat ovarian cancer and/or breast cancer. From carrying out cancer drug repositioning for the FDA- and CFDA-approved drug estrone, experiments for this invention show, based on screening of non-anti-cancer drugs for various cancer cell lines (tissue types) and mutation sites, that estrone has a new use as an anti-ovarian cancer and/or anti-breast cancer medication, thus achieving a new purpose for an old drug.
    Type: Grant
    Filed: June 30, 2015
    Date of Patent: May 21, 2019
    Assignee: SHANGHAI JIAO TONG UNIVERSITY
    Inventors: Yongyong Shi, Zhijian Song
  • Patent number: 10295549
    Abstract: The present invention relates to methods of diagnosing samples as well as various microfluidic, microcentrifuge and microfilter devices. In one embodiment, the present invention provides a method of diagnosing neurodegenerative diseases using mitochondrial and/or platelet samples. In another embodiment, the present invention provides a microfluidic device that selectively captures and analyzes a desired amount of target biological particle.
    Type: Grant
    Filed: September 1, 2016
    Date of Patent: May 21, 2019
    Assignee: DIGNITY HEALTH
    Inventor: Jonathan E. Valla