Measuring Or Testing Process Involving Enzymes Or Micro-organisms; Composition Or Test Strip Therefore; Processes Of Forming Such Composition Or Test Strip Patents (Class 435/4)
  • Patent number: 11662342
    Abstract: The current invention relates to an improved method for measuring modulating effects of compounds on epithelial cell barrier function. The methods allows for high throughput screening of test compounds individually or in combination. Compounds improving the epithelial barrier function or compounds negatively influencing epithelial barrier function can be analyzed with the method described herein.
    Type: Grant
    Filed: July 8, 2016
    Date of Patent: May 30, 2023
    Assignee: MIMETAS B.V.
    Inventors: Paul Vulto, Sebastiaan Johannes Trietsch, Henriëtte Leonore Lanz, Marianne Katharina Vormann
  • Patent number: 11610650
    Abstract: Methods of designing a polynucleotide sequence for expressing a polypeptide-of-interest in a cell are provided. Also provided are artificial transcript sequences generated according to the present teachings. Further provided are methods of estimating the adaptiveness of a transcript sequence encoding a polypeptide-of-interest to a gene expression machinery in a cell.
    Type: Grant
    Filed: May 28, 2015
    Date of Patent: March 21, 2023
    Assignee: Ramot at Tel-Aviv University Ltd.
    Inventors: Tamir Tuller, Hadas Zur
  • Patent number: 11597905
    Abstract: An automated cell culture and tissue engineering system comprising defined and separate environmental zones provide for increased control and maintenance of the internal environment of the system such that the temperature, air flow and gases surrounding the bioreactor module form one zone that is maintained separately to a second zone formed surrounding the reagent fluid reservoir. The system further comprises means for elimination and/or management of condensation within the second zone of the system.
    Type: Grant
    Filed: December 26, 2019
    Date of Patent: March 7, 2023
    Inventors: Guy Oram, Taylor Plant, Ian Grant, Timothy Smith
  • Patent number: 11566217
    Abstract: An automated cell culture system with one or more pumps configured to operate on a duty cycle prevents excess heat generation, allowing the cell culture system to operate inside a conventional incubator without overheating. The duty cycle involves switching the pump between on and off modes. By running pumps for a short period of time and then shutting them off, less heat is produced. To account for the reduced pumping time during the cycle, the pump can be run at a higher flow rate while it is on, so that the average flow rate over the course of the cycle is not reduced. Systems of the invention employ duty cycles in which the on-cycle is shorter than the off-cycle, and particularly where the on-cycle is less than 20% of the duration of the entire duty cycle.
    Type: Grant
    Filed: August 13, 2019
    Date of Patent: January 31, 2023
    Assignee: FLASKWORKS, LLC
    Inventor: Shashi K. Murthy
  • Patent number: 11548011
    Abstract: The disclosure describes systems and methods for separating a plurality of molecular entities with differing densities. The system includes: a pair of magnetic poles of like polarity to provide a magnetic field; and a container holding the plurality of molecular entities in a fluid medium comprising nanoparticles that substantially change a magnetic susceptibility of the fluid medium such that, when the container is placed inside the magnetic field, sufficient gradients in an effective density of the fluid medium are generated inside the container to levitate the plurality of molecular entities to respective layers within the container, each respective layer corresponding to a respective density.
    Type: Grant
    Filed: July 23, 2019
    Date of Patent: January 10, 2023
    Assignee: The Brigham and Women's Hospital, Inc.
    Inventor: Morteza Mahmoudi
  • Patent number: 11535828
    Abstract: Provided is a three-dimensional tissue, including: a first cellular region including cells of a first type; and a second cellular region including cells of a second type different from the first type, wherein the cells of the first type are cells that emit light by chemiluminescence, bioluminescence, or fluorescence in response to an external stimulus.
    Type: Grant
    Filed: May 10, 2017
    Date of Patent: December 27, 2022
    Assignee: RICOH COMPANY, LTD.
    Inventors: Waka Lin, Aino Hasegawa, Natsuko Hemmi, Satoshi Izumi, Shusaku Shiomoto
  • Patent number: 11519006
    Abstract: The present invention relates generally to methods and compositions for gene therapy and immunotherapy that activate gamma delta T-cells, and in particular, can be used in the treatment of various cancers and infectious diseases.
    Type: Grant
    Filed: September 6, 2019
    Date of Patent: December 6, 2022
    Assignee: American Gene Technologies International Inc.
    Inventors: Charles David Pauza, Haishan Li, Tyler Lahusen, Mei-Ling Liou
  • Patent number: 11513242
    Abstract: A radiation detector is generally described. The detector can comprise a thallium halide (e.g., TlBr) and/or an indium halide. The thallium halide and/or indium halide can be doped with a dopant or a mixture of dopants. The dopant can comprise an alkaline earth metal element, a lanthanide element, and/or an element with an oxidation state of +2. Non-limiting examples of suitable dopants include Ba, Sr, Ca, Mg, La, Ce, Pr, Nd, Pm, Sm, Eu, Gd, Tb, Dy, Ho, Er, Tm, and/or Yb. Radiation detectors, as described herein, may have beneficial properties, including enhanced charge collection and long-term stability.
    Type: Grant
    Filed: March 4, 2015
    Date of Patent: November 29, 2022
    Assignee: Radiation Monitoring Devices, Inc.
    Inventors: Kanai S. Shah, Leonard Cirignano, Hadong Kim, Alexei Churilov, Andrey Gueorguiev, Alireza Kargar
  • Patent number: 11510587
    Abstract: A method for delineating a ventricle from MRI data relating to the heart of a patient, the method comprising: a) providing a contrast-enhanced cine MRI dataset; b) providing one or more additional MRI datasets; c) segmenting one or more features on the additional MRI dataset or datasets; d) mapping the segmented features to the contrast-enhanced cine MRI dataset; and e) using the segmented features as mapped in step d) to assist segmentation of the ventricle on the contrast-enhanced cine MRI dataset. A corresponding device and computer program are also disclosed.
    Type: Grant
    Filed: October 22, 2018
    Date of Patent: November 29, 2022
    Assignee: Pie Medical Imaging B.V.
    Inventors: Wisnumurti Kristanto, Jean-Paul Aben
  • Patent number: 11515007
    Abstract: The invention relates to an in silico screening method to identify candidate excipients for reducing aggregation of a protein in a formulation. The method combines computational molecular modeling and molecular dynamics simulations to identify sites on a protein where non-specific self-interaction and interaction of different test excipients may occur, determine the relative binding energies of such interactions, and select one or more test excipients that meet specified interaction criteria for use as candidate excipients in empirical screening studies.
    Type: Grant
    Filed: March 6, 2017
    Date of Patent: November 29, 2022
    Assignee: Merck Sharp & Dohme LLC
    Inventors: Francis Insaidoo, David Roush
  • Patent number: 11506606
    Abstract: In-situ fluorimeters and methods and systems for collecting and analyzing sensor data to predict water source contamination are provided. In one embodiment, a method is provided that includes receiving sensor data regarding a water source. Changepoints may then be calculated within the sensor data and the sensor data may be split into intervals at the changepoints. A machine learning model may then be used to classify the intervals and a predicted contamination event for the water source may be identified based on the classified intervals. In another embodiment, an in-situ fluorimeter is provided. The in-situ fluorimeter comprises one or more UV LEDs centered around a pre-set excitation wavelength (e.g., a TLF excitation wavelength), a bandpass filter, a lens, a photodiode system, a machine learning platform; and an alarm triggered by contamination events, wherein the alarm is calibrated through the machine learning system.
    Type: Grant
    Filed: February 28, 2020
    Date of Patent: November 22, 2022
    Assignee: SWEETSENSE, INC.
    Inventors: Emily Bedell, Katie Frankhauser, Taylor Sharpe, Daniel Wilson, Evan Thomas
  • Patent number: 11493428
    Abstract: Described herein are microfluidic devices and methods that can greatly improve cell quality, streamline workflows, and lower costs. Applications include research and clinical diagnostics in cancer, infectious disease, and inflammatory disease, among other disease areas.
    Type: Grant
    Filed: March 14, 2014
    Date of Patent: November 8, 2022
    Inventors: Michael Grisham, Curt I Civin, James C. Sturm, Robert H. Austin, Joseph D'Silva, Yu Chen
  • Patent number: 11492613
    Abstract: Provided are methods for screening a desired variant of a target gene in a eukaryotic system. Compositions for screening a desired variant of a target gene are also provided.
    Type: Grant
    Filed: August 26, 2020
    Date of Patent: November 8, 2022
    Inventors: Alfonso Farruggio, Ruby Yanru Tsai, Lingjie Kong
  • Patent number: 11480578
    Abstract: The present invention concerns a method for identifying and using a biomarker, or creating a proteome profile, indicative of a reduced response to a drug in a patient involving a thermal shift assay on a sample. The method comprises the steps of a) heating a sample from a patient b) separating soluble from insoluble protein, c) analysing either or both the soluble and insoluble protein fractions of step b) to determine the melting temperature.
    Type: Grant
    Filed: March 25, 2015
    Date of Patent: October 25, 2022
    Inventors: Daniel Martinez Molina, Par Nordlund
  • Patent number: 11467117
    Abstract: There is provided an impedance sensor capable of counting the number of microscopic biological materials and specifying their properties stably with high sensitivity. An impedance sensor includes a measuring electrode pair formed at a wiring layer in a multilayer-wiring circuit board and one or more dielectrophoresis electrodes formed at another wiring layer lower than the wiring layer.
    Type: Grant
    Filed: June 4, 2018
    Date of Patent: October 11, 2022
    Inventor: Nobuyuki Ashida
  • Patent number: 11454627
    Abstract: A device suitable for the detection and/or characterization of target particles in a fluid is disclosed. The device comprises: at least one heating element for heating and/or measuring a temperature, the heating element comprising a core comprising at least one electrically conducting portion, an electric isolating layer provided at a surface of the core and electrically isolates the core from the sample, and a plurality of binding sites at/to which target particles can bind. The device further comprising a processing means configured to measure an electric output of the least one heating element, a change of the electric output of the at least one heating element and/or its heating power and for deriving, based thereon, a characteristic of the target particles.
    Type: Grant
    Filed: January 26, 2018
    Date of Patent: September 27, 2022
    Inventors: Peter Cornelis, Mehran Khorshid, Gideon Wackers, Patrick Wagner
  • Patent number: 11434515
    Abstract: Apparatus and methods are described including introducing a cell suspension comprising red blood cells into a carrier that is a closed cavity that includes a base surface, via an inlet defined by the carrier. The cells in the cell suspension are allowed to settle on the base surface of the carrier to form a monolayer of cells on the base surface of the carrier. At least one microscope image of at least a portion of the monolayer of cells is acquired. Other applications are also described.
    Type: Grant
    Filed: August 21, 2018
    Date of Patent: September 6, 2022
    Assignee: S.D. Sight Diagnostics Ltd.
    Inventors: Joseph Joel Pollak, Arnon Houri Yafin, Seth J. Salpeter
  • Patent number: 11408882
    Abstract: In the various illustrative embodiments herein, test devices are described with opposing sensor arrays and same side contacts.
    Type: Grant
    Filed: August 7, 2020
    Date of Patent: August 9, 2022
    Assignee: Siemens Healthcare Diagnostics Inc.
    Inventors: Jennifer A. Samproni, Todd Andrade, Marlene Shi
  • Patent number: 11400117
    Abstract: The present invention relates to T cell compositions and methods of using the same in the context of therapy and treatment. In particular, the invention provides T cells that are modified (e.g., genetically and/or functionally) to maintain functionality under conditions in which unmodified T cells display exhaustion. Compositions and methods disclosed herein find use in preventing exhaustion of engineered (e.g., chimeric antigen receptor (CAR) T cells) as well as non-engineered T cells thereby enhancing T cell function (e.g., activity against cancer or infectious disease).
    Type: Grant
    Filed: December 14, 2018
    Date of Patent: August 2, 2022
    Assignee: The Board of Trustees of the Leland Stanford Junior University
    Inventors: Crystal Mackall, Rachel Lynn, Evan Weber, Elena Sotillo
  • Patent number: 11395635
    Abstract: A catastrophe-theoretic approach is provided for predicting an occurrence of an acute inflammatory condition or event (e.g., SIRS or sepsis) for a human patient based on a time series of monitored vital signs values measured from a patient, and in some instances, for providing advanced notice to clinicians or caregivers when such an acute inflammatory event is forecasted or modifying treatment for the patient, according to the predicted likelihood. In particular, an acute inflammatory condition management system is provided for determining a likelihood of near-term future significant acute inflammation in human patients. Embodiments of the disclosure described herein may provide a forecasted risk for future significant acute inflammation within a time horizon comprising a future time interval. In one embodiment, the future time interval is from 30 min to approximately 8 hours into the future, and may be dependent on the frequency of vital signs measurements.
    Type: Grant
    Filed: December 31, 2018
    Date of Patent: July 26, 2022
    Inventor: Douglas S. McNair
  • Patent number: 11364503
    Abstract: A microfluidic device may, in an example, include at least one microfluidic channel, a dielectrophoresis separator to separate a plurality of cells passing within the at least one microfluidic channel, and a thermal resistor to eject at least one cell from the microfluidic device. A cassette may, in an example, include a die coupled to a substrate of the cassette, the die including at least one microfluidic channel, a dielectrophoresis separator along the microfluidic channel to separate a plurality of cells passing within the microfluidic channel, and an ejection device to eject at least one of the plurality of cells into an assay well.
    Type: Grant
    Filed: July 17, 2017
    Date of Patent: June 21, 2022
    Assignee: Hewlett-Packard Development Company, L.P.
    Inventors: Viktor Shkolnikov, Kenneth Ward
  • Patent number: 11366111
    Abstract: The present invention relates to a method for improving the detection and diagnosis targeting biomarkers using an aqueous two-phase system (ATPS). In one embodiment, the present invention provides a method for improving a diagnostic procedure involving the detection or quantification of one or more biomarkers in liquid biopsy, the method comprises contacting a porous material embedded with ATPS components with a liquid biopsy containing the biomarkers, purifying and concentrating the target biomarkers, and providing a product comprising the target biomarkers for performing the diagnostic procedure.
    Type: Grant
    Filed: March 28, 2018
    Date of Patent: June 21, 2022
    Inventor: Yin To Chiu
  • Patent number: 11365386
    Abstract: There is described a cellular behaviour monitoring device for monitoring changes in behaviour of cells contained in a sample. The device generally has a well plate with a sample receiving well recessed therein. A filter membrane extends across the well plate and hermetically covers the sample receiving well. The filter membrane has nutrient-permeable and cell-impermeable pores extending through the filter membrane. The device has an electrode layer extending across the filter membrane. The electrode layer has a substrate and behaviour monitoring electrodes spaced-apart from one another in a region of the substrate, with the region being aligned with the sample receiving well. The electrode layer has nutrient-permeable apertures distributed across the region, with at least some of pores are aligned with at least some of the apertures to allow fluid communication therebetween, and nutrient exchange between the sample receiving well and a surrounding environment.
    Type: Grant
    Filed: May 3, 2019
    Date of Patent: June 21, 2022
    Inventors: Benoit Gosselin, Younès Messaddeq, Jacques Corbeil, Eric Bharucha
  • Patent number: 11366086
    Abstract: The invention belongs to the technical field of natural medicinal chemistry and quality control thereof, and relates to a method for determining the weight average molecular weight and the purity of a soluble salt of an acidic saccharide. The method comprises using metal ion content in the soluble salt of an acidic saccharide to correct the weight average molecular weight and the content of the of acid saccharide obtained by the combined use of the molecular sieve chromatography and a multi-angle laser scattering detector SEC-MALS. The method of the present invention can be used to more quickly and accurately determine the weight average molecular weight and content of acidic saccharide soluble salts.
    Type: Grant
    Filed: September 6, 2018
    Date of Patent: June 21, 2022
    Inventors: Zhenqing Zhang, Yangyang Zeng
  • Patent number: 11358984
    Abstract: In situ Raman spectroscopy methods and systems for characterizing or quantifying a protein purification intermediate and/or final concentrated pool during production or manufacture are provided. In one embodiment, in situ Raman spectroscopy is used to characterize or quantify protein purification intermediates critical quality attributes during downstream processing (i.e., after harvest of the protein purification intermediate). For example, the disclosed in situ Raman spectroscopy methods and systems can be used to characterize and quantify protein purification intermediates as the protein purification intermediates are purified, condensed, or otherwise formulated into the final drug product to be sold or administered.
    Type: Grant
    Filed: August 26, 2019
    Date of Patent: June 14, 2022
    Assignee: Regeneran Pharmaceuticals, Inc.
    Inventors: Christina Passno, Christopher Cowan, Andrew Tustian
  • Patent number: 11338290
    Abstract: Systems and methods for levitating populations of moieties, cells, or other such units using one or more magnets in a microfluidic environment are provided. These systems and methods may be used to, for example, separate or sort heterogeneous populations of the units from one another, to assembly a multi-unit assembly during the levitating of the units, and to evaluate samples at the point of care in real-time. These systems and methods may also utilize a frame that enables an imaging device, such as a smartphone, to capture the units in real time as they are manipulated in the system.
    Type: Grant
    Filed: September 30, 2016
    Date of Patent: May 24, 2022
    Assignee: The Board of Trustees of the Leland Stanford Junior University
    Inventors: Murat Baday, Naside Gozde Durmus, Semih Calamak, Utkan Demirci, Ronald W. Davis, Lars Steinmetz, Jaeyoung Yang, Thiruppathiraja Chinnasamy, Alessandro Tocchio
  • Patent number: 11342045
    Abstract: The present invention relates to a method and system for dynamically analyzing, determining, predicting and displaying ranked suitable heterologous biosynthesis pathways for a specified host. The present invention addresses the problem of finding suitable pathways for the endogenous metabolism of a host organism because the efficacy of heterologous biosynthesis is affected by competing endogenous pathways. The present invention is called MRE (Metabolic Route Explorer), and it was conceived and developed to systematically and dynamically search for, determine, analyze, and display promising heterologous pathways while considering competing endogenous reactions in a given host organism.
    Type: Grant
    Filed: February 2, 2017
    Date of Patent: May 24, 2022
    Inventors: Xin Gao, Hiroyuki Kuwahara, Meshari Saud Alazmi, Xuefeng Cui
  • Patent number: 11326146
    Abstract: The present invention relates to compositions comprising low sugar media, the methods of using these compositions to reduce trauma and stress on processed animal sperm, the resulting sperm and embryo products, and the methods of use of these products to increase the quality, quantity and viability of progeny and improved rates of births in animals.
    Type: Grant
    Filed: November 20, 2015
    Date of Patent: May 10, 2022
    Assignee: Inguran, LLC
    Inventors: Thomas B. Gilligan, Ramakrishnan Vishwanath, Kilby Willenburg
  • Patent number: 11320442
    Abstract: Disclosed is a method for body fluid-based neurodegenerative disease diagnosis through high-sensitive immunoassay of aggregated proteins by photooxidation-induced amplification. The method according to the present disclosure provides an effect of quantitatively analyzing aggregated proteins in the form of oligomers or monomers which are present in trace amounts in a body fluid and measures normal or abnormal protein aggregation by detecting the aggregated proteins in the form of oligomers or monomers with high sensitivity by reaction of antibody-conjugated enzymes selectively bound to the aggregated proteins with substrates and photooxidation-induced amplification, thereby allowing accurate diagnosis of a neurodegenerative disease.
    Type: Grant
    Filed: April 19, 2017
    Date of Patent: May 3, 2022
    Inventors: Ji Yoon Kang, Min Cheol Park, Youhee Heo
  • Patent number: 11310239
    Abstract: A network connection method, a hotspot terminal, and a management terminal are provided. A hotspot terminal receives an Internet access request sent by a mobile terminal, sends an input request to the mobile terminal according to the received Internet access request; receives user identity information sent by the mobile terminal; receives a permission confirmation instruction that is entered by an administrator according to the user identity information; determines Internet access permission of the mobile terminal according to the received permission confirmation instruction. According to the network connection method provided in the embodiments of the present application, the tedious and complex process where a mobile terminal logging into a remote network by using a hotspot terminal is significantly improved.
    Type: Grant
    Filed: February 4, 2020
    Date of Patent: April 19, 2022
    Assignee: Huawei Technologies Co., Ltd.
    Inventor: Zhen Zhong
  • Patent number: 11304686
    Abstract: A guidance system and method include a real-time imaging device (121) configured to intra-operatively collect image data of a target region to provide a real-time image. A target determination module (108) is configured to generate an overlay map (111) registered to the real-time image to project morphological feature information on corresponding features in the real-time image of the target region. A medical instrument (122) is provided to perform a procedure on the target region such that the at least one medical instrument is guided to a region suitable for performing an operative task based upon the overlay map.
    Type: Grant
    Filed: June 11, 2012
    Date of Patent: April 19, 2022
    Inventors: Robert Manzke, Raymond Chan, Haytham Elhawary, Aleksandra Popovic
  • Patent number: 11301536
    Abstract: The present disclosure is directed to associating computing devices with each other based on computer network activity for selection of content items as part of an online content item placement campaign. A first linking factor is identified based on a connection between a first device and the computer network via a first IP address during a first time period, and based on a connection between a second device and the computer network via the first IP address during the first time period. A number of devices that connect with the computer network via the first IP address is determined. A positive match probability is generated. A second and third linking factors are monitored. A negative match probability is determined based on the second and third linking factors. The first device is linked with the second device based on the positive and negative match probabilities.
    Type: Grant
    Filed: March 6, 2020
    Date of Patent: April 12, 2022
    Assignee: GOOGLE LLC
    Inventor: Jianjun Qiu
  • Patent number: 11298061
    Abstract: A specimen mixing and transfer device adapted to receive a sample is disclosed. The specimen mixing and transfer device includes a housing, a material including pores that is disposed within the housing, and a dry anticoagulant powder within the pores of the material. In one embodiment, the material is a sponge material. In other embodiments, the material is an open cell foam. In one embodiment, the material is treated with an anticoagulant to form a dry anticoagulant powder finely distributed throughout the pores of the material. A blood sample may be received within the specimen mixing and transfer device. The blood sample is exposed to and mixes with the anticoagulant powder while passing through the material.
    Type: Grant
    Filed: September 22, 2015
    Date of Patent: April 12, 2022
    Assignee: Becton, Dickinson and Company
    Inventors: Milan Ivosevic, Alexander James Blake, Ryan W. Muthard
  • Patent number: 11293061
    Abstract: Embodiments of the present disclosure relate to sequencing methods using nucleotide molecules with a 3? AOM blocking group, which can provide reduced phasing and pre-phasing values. Also provided herein are kits related to such sequencing methods.
    Type: Grant
    Filed: December 20, 2019
    Date of Patent: April 5, 2022
    Assignee: Illumina Cambridge Limited
    Inventors: Antoine Francais, Elena Cressina, Adam Culley, Angelica Mariani, Xiaolin Wu, Xiaohai Liu
  • Patent number: 11285475
    Abstract: A device and method for fluorescent labelling a component is provided. The device comprising a first user-replaceable reservoir comprising a strongly buffered alkaline solution of aromatic ortho-dialdehyde dye, a second user-replaceable reservoir comprising a weakly buffered acidic solution of a reducing agent, one or more fluid pathways comprising the component, and a network of connection channels linking the reservoirs and the fluid pathway to enable the alkaline solution and the acidic solution to combine with the component in order to label the component by reacting the component with the alkaline solution and the acidic solution.
    Type: Grant
    Filed: September 11, 2017
    Date of Patent: March 29, 2022
    Assignee: Fluidic Analytics Limited
    Inventors: Tuomas Pertti Jonathan Knowles, Sean Devenish
  • Patent number: 11280796
    Abstract: The present invention relates to methods of diagnosing samples as well as various microfluidic, microcentrifuge and microfilter devices. In one embodiment, the present invention provides a method of diagnosing neurodegenerative diseases using mitochondrial and/or platelet samples. In another embodiment, the present invention provides a microfluidic device that selectively captures and analyzes a desired amount of target biological particle.
    Type: Grant
    Filed: April 2, 2019
    Date of Patent: March 22, 2022
    Assignee: Dignity Health
    Inventor: Jonathan E. Valla
  • Patent number: 11278657
    Abstract: Cerebrospinal fluid (CSF) and other fluid amelioration systems completely or partially implantable within a mammalian subject and associated methods include a substrate and an agent for amelioration of a toxic biomolecule present in the CSF or fluid, wherein the agent is disposed on or within the substrate.
    Type: Grant
    Filed: October 2, 2020
    Date of Patent: March 22, 2022
    Assignee: EnClear Therapies, Inc.
    Inventors: Anthony DePasqua, Kevin Eggan, Jr., Kevin Kalish, Manuel A. Navia, Kasper Roet, Ching-Hua Tseng, Alan D. Watson, William X. Siopes, Gianna N. Riccardi, Marcie Ann Glicksman
  • Patent number: 11276482
    Abstract: The present invention provides a device and system for monitoring the accuracy of procedures in the course of the performance of a task, the task comprising at least one procedure to be performed, the device comprising: an input interface for receiving input data relating to the procedures; a data store for storing data relating to the procedures; a processor for: comparing the input data with the stored data; and generating a comparison result indicating the result of that comparison; and an output interface for outputting the comparison result.
    Type: Grant
    Filed: June 14, 2016
    Date of Patent: March 15, 2022
    Inventors: Eduardo Vom, Jeremy Phillip Stimson, Yassaman Pouladi, Ben Hobbs, Tammie Kim Roy, Jan Kirsten
  • Patent number: 11275080
    Abstract: A method of analyzing a biological sample from a subject that has a tumor or cancer, comprising: determining, for target cells having a phenotype of interest spatial resolution of the target cells, density of the spatially resolved target cells in the sample; and proximity between spatially resolved target cells of interest in the sample; and determining an overall score based at least in part on the preceding parameters. A method for identifying a patient as a responder to single agent anti-PD-1 or anti-PD-L1 therapy is provided. Similar methods are provided for detecting adaptive immune resistance, the presence of cancer in a patient sample, determining efficacy of cancer therapy, and determining response to and monitoring the efficacy of cancer therapy.
    Type: Grant
    Filed: November 5, 2015
    Date of Patent: March 15, 2022
    Inventors: Paul C. Tumeh, Antoni Ribas
  • Patent number: 11261454
    Abstract: The present invention relates to a method for introducing a polynucleotide into non-adhesively growing plant cells, comprising the following steps: providing a solid support having immobilized thereto the polynucleotide in dry state; contacting the plant cells with the polynucleotide on the solid support so as to obtain transformed plant cells; and optionally washing the plant cells.
    Type: Grant
    Filed: April 9, 2019
    Date of Patent: March 1, 2022
    Assignee: ScreenSYS GmbH
    Inventors: Oleksandr Dovzhenko, Claude Becker, Karsten Voigt, Klaus Palme
  • Patent number: 11261487
    Abstract: The claimed invention relates to a method of processing a polynucleotide, by obtaining a sense polynucleotide strand comprising a homopolymeric region that is longer that the reading section of a nanopore; synthesizing an antisense polynucleotide strand under conditions in which a nucleotide analog is incorporated at random in a reverse complement of the homopolymer region, such that the length of the homopolymer region in the antisense polynucleotide strand is shorter than the reading section of the nanopore; and moving the antisense polynucleotide strand through the nanopore such that a proportion of the antisense polynucleotide strand interacts with the nanopore.
    Type: Grant
    Filed: February 5, 2020
    Date of Patent: March 1, 2022
    Assignee: Oxford Nanopore Technologies PLC
    Inventors: Clive Gavin Brown, James Anthony Clarke, Graham Hall, Gavin Harper, Andrew John Heron, James White
  • Patent number: 11256994
    Abstract: A system and method that predicts whether a given protein-ligand pair is active or inactive and outputs a pose score classifying the propriety of the pose. A 3D bioactivity platform comprising a 3D bioactivity module and data platform scrapes empirical lab-based data that a docking simulator uses to generate a dataset from which a 3D-CNN model is trained. The model then may receive new protein-ligand pairs and determine a classification for the bioactivity and pose propriety of that protein-ligand pair. Furthermore, gradients relating to the binding affinity in the 3D model of the molecule may be used to generate profiles from which new protein targets may be determined.
    Type: Grant
    Filed: March 16, 2021
    Date of Patent: February 22, 2022
    Assignee: RO5 INC.
    Inventors: Alwin Bucher, Aurimas Pabrinkis, Orestis Bastas, Mikhail Demtchenko, Zeyu Yang, Cooper Stergis Jamieson, {hacek over (Z)}ygimantas Jo{hacek over (c)}ys, Roy Tal, Charles Dazler Knuff
  • Patent number: 11255809
    Abstract: The present invention relates to droplet-based surface modification and washing. According to one embodiment, a method of splitting a droplet is provided, the method including providing a droplet microactuator including a droplet including one or more beads and immobilizing at least one of the one or more beads. The method further includes conducting one or more droplet operations to divide the droplet to yield a set of droplets including a droplet including the one or more immobilized beads and a droplet substantially lacking the one or more immobilized beads.
    Type: Grant
    Filed: February 11, 2020
    Date of Patent: February 22, 2022
    Assignees: Advanced Liquid Logic, Inc., Duke University
    Inventors: Vamsee K. Pamula, Vijay Srinivasan, Michael G. Pollack, Richard B. Fair
  • Patent number: 11249070
    Abstract: A method of assessing an anti-cancer drug including culturing a cell structure including cancer cells and stromal cells in a presence of at least one anti-cancer drug, and assessing an anti-cancer effect of the at least one anti-cancer drug based on a number of viable cancer cells in the cell structure after the culturing.
    Type: Grant
    Filed: October 19, 2018
    Date of Patent: February 15, 2022
    Inventors: Shiro Kitano, Kei Tsukamoto, Shinji Irie
  • Patent number: 11224586
    Abstract: The present invention relates to a compound of the following general formula (I); or a pharmaceutically acceptable salt and/or solvate thereof, for use as drug, particularly intended for inhibiting a programmed cell death route selected from the group consisting of ferroptosis, oxytosis and cellular necroptosis. The present invention also relates to a compound of general formula (I) for use as a drug for neuroprotection as well as for preventing and/or treating disorders associated with cellular necroptosis or ferroptosis. The present invention also relates to a pharmaceutical composition comprising a compound of general formula (I), or a pharmaceutically acceptable salt and/or solvate thereof. The present invention also encompasses the use of a compound of the general formula (I) for organs preservation.
    Type: Grant
    Filed: October 18, 2017
    Date of Patent: January 18, 2022
    Assignees: Institut National de la Sante et de la Recherche Medicale (INSERM), Centre National de la Recherche Scientifique (CNRS), Sorbonne Universite, Universite de Poitiers, Centre Hospitalier Universitaire de Poitiers
    Inventors: Stéphane Bach, Marie-Thérèse Dimanche-Boitrel, Claire Delehouze, Thierry Hauet
  • Patent number: 11215608
    Abstract: The present invention provides methods of determining cell sensitivity to a therapeutic agent.
    Type: Grant
    Filed: October 26, 2016
    Date of Patent: January 4, 2022
    Assignee: Dana-Farber Cancer Institute, Inc.
    Inventor: Anthony Letai
  • Patent number: 11208698
    Abstract: Embodiments of this invention include methods for detection of markers of bladder cancer and inflammatory conditions of the bladder. Particularly, methods include detection of expression of certain genetic markers for bladder cancer and markers for detection of inflammatory conditions of the bladder. These methods provide improved detection of the markers, and provide better detection of bladder cancer and inflammatory conditions of the bladder.
    Type: Grant
    Filed: May 24, 2018
    Date of Patent: December 28, 2021
    Inventors: Parry John Guilford, Mark Dalphin, Laimonis Kavalieris, Paul O'Sullivan
  • Patent number: 11203015
    Abstract: The present invention relates to a microfluidic device for creating within a cell assembly a cell-free area, comprising at least one cell chamber, wherein the at least one cell chamber comprises: —a fluid inlet for introducing fluid into the cell chamber, —a first area, —a second area, —at least one mechanical excluding means for excluding cells from the first area of the chamber and being operable between an excluding position and a releasing position optionally via an actuation line, wherein the second area of the cell chamber is outside of the operation range of the mechanical excluding means.
    Type: Grant
    Filed: April 12, 2017
    Date of Patent: December 21, 2021
    Inventors: Drago Sticker, Peter Ertl, Sarah Lechner
  • Patent number: 11203016
    Abstract: In accordance with embodiments herein a method for capturing cells of interest in a digital microfluidic system is provided, comprising utilizing a droplet actuator to transport a sample droplet to a microwell device. The microwell device includes a substrate having a plurality of microwells that open onto a droplet operations surface of the microwell device. The sample droplet includes cells of interest that enter the microwells. The method introduces capture beads to the microwells, and the capture elements are immobilized on the capture beads. The method utilizes the droplet actuator to transport a cell lysis reagent droplet to the microwell device. Portions of the cell lysis reagent droplet enter the microwells and, during an incubation period, cause the cells of interest to release analyte that is captured by the capture elements on the capture beads.
    Type: Grant
    Filed: November 30, 2016
    Date of Patent: December 21, 2021
    Assignee: Illumina, Inc.
    Inventors: Arash Jamshidi, Yan-you Lin, Farnaz Absalan, Sarah Stuart, Gordon Cann, Yir-Shyuan Wu, Tarun Khurana, Jeffrey S Fisher
  • Patent number: 11200446
    Abstract: Methods and systems for image analysis are provided, and in particular for identifying a set of base-calling locations in a flow cell for DNA sequencing. These include capturing flow cell images after each sequencing step performed on the flow cell, and identifying candidate cluster centers in at least one of the flow cell images. Intensities are determined for each candidate cluster center in a set of flow cell images. Purities are determined for each candidate cluster center based on the intensities. Each candidate cluster center with a purity greater than the purity of the surrounding candidate cluster centers within a distance threshold is added to a template set of base-calling locations.
    Type: Grant
    Filed: March 31, 2021
    Date of Patent: December 14, 2021
    Assignee: Element Biosciences, Inc.
    Inventors: Chunhong Zhou, Semyon Kruglyak, Francisco Garcia, Minghao Guo, Haosen Wang, Ryan Kelly