Measuring Or Testing Process Involving Enzymes Or Micro-organisms; Composition Or Test Strip Therefore; Processes Of Forming Such Composition Or Test Strip Patents (Class 435/4)
  • Patent number: 10228348
    Abstract: Provided is a solid state support structure including an aperture having a molecular entrance and a molecular exit. A first reservoir is in fluidic communication with the molecular entrance of the aperture and contains a molecule-bearing liquid solution. A second reservoir is in fluidic communication with the molecular exit of the aperture for containing a molecule-bearing liquid solution. A first liquid channel is connected to the first reservoir within less than about 300 microns of the aperture in the support structure and includes molecule-bearing liquid solution for delivery to the first reservoir. A second liquid channel is connected to the second reservoir for accepting molecule-bearing liquid solution from the second reservoir. An electrical connection between the first reservoir and the second reservoir imposes an electrical bias between the first reservoir and the second reservoir for driving the molecule-bearing liquid solution through the aperture in the solid state support structure.
    Type: Grant
    Filed: January 22, 2016
    Date of Patent: March 12, 2019
    Assignee: President and Fellows of Harvard College
    Inventors: Daniel Branton, Jene A Golovchenko
  • Patent number: 10222377
    Abstract: Use of BUBR1 as a biomarker for predicting the response to a compound, preferably resistance of a disease such as cancer in a subject, wherein the compound is a furazanobenzimidazole compound of general formula (I).
    Type: Grant
    Filed: January 19, 2012
    Date of Patent: March 5, 2019
    Inventors: Heidi Alexandra Lane, Felix Bachmann, Madlaina Breuleux, Michael Boutros, Daniel Gilbert, Xian Zhang
  • Patent number: 10215728
    Abstract: A bioFET cell for measuring a time dependent characteristic of an analyte bearing fluid includes a source, a drain, a semiconductive single wall carbon nanotube network layer extending between the source and drain electrodes and electrically coupled there between, a gate insulatively spaced from and disposed over and extending between the source and drain electrodes, a layer of at least one selected antibody disposed on and linked to the polymer layer to functionalize the semiconductive single wall carbon nanotube network layer to a selected target biomarker corresponding to the at least one selected antibody so that electron transport into the semiconductive single wall carbon nanotube network layer is facilitated, where the source, drain and gate electrodes with the carbon nanotube network layer form a defined channel through which the analyte bearing fluid may flow, and a high impedance source follower amplifier coupled to the source electrode.
    Type: Grant
    Filed: October 4, 2017
    Date of Patent: February 26, 2019
    Assignee: Sensor Kinesis Corporation
    Inventor: Josh Shachar
  • Patent number: 10206940
    Abstract: The invention relates to compositions of nicotinoyl ribosides and nicotinamide riboside derivatives and their methods of use. In some embodiments, the invention relates to methods of snaking nicotinoyl ribosides. In some embodiments, the invention relates to pharmaceutical compositions and nutritional supplements containing a nicotinoyl riboside. In further embodiments, the invention relates to methods of using nicotinoyl ribosides and nicotinamide riboside derivatives that promote the increase of intracellular levels of nicotinamide adenine dinucleotide (NAD+) in cells and tissues for improving cell and tissue survival.
    Type: Grant
    Filed: April 25, 2016
    Date of Patent: February 19, 2019
    Inventors: Anthony A. Sauve, Tianle Yang Redanz
  • Patent number: 10209240
    Abstract: This present invention provides rapid, reproducible, biomarker-based screening methods for the developmental toxicity testing of compounds. The methods are designed to identify the exposure level at which a test compound perturbs metabolism in a manner predictive of developmental toxicity. In particular, the perturbation of two metabolites, ornithine and cystine, is measured, wherein a ratio of the fold change in ornithine to the fold change in cystine of less than or equal to about 0.88 is indicative of the teratogenicity of a test compound.
    Type: Grant
    Filed: December 20, 2017
    Date of Patent: February 19, 2019
    Assignee: Stemina Biomarker Discovery, Inc.
    Inventors: Alan Smith, Paul West, Jessica Palmer
  • Patent number: 10210312
    Abstract: Example methods of quantifying known and unknown risks of an adverse drug event in an individual based on various factors are disclosed. In some embodiments, factors include known drug-drug interactions and unknown phenotypes of cytochromes. Quantification may be based on severity of the adverse drug event/and or probability of occurrence in some embodiments. Example methods of displaying the quantified risk are also disclosed. In one embodiment, the risk of individuals is aggregated to display the risk of a population.
    Type: Grant
    Filed: January 31, 2014
    Date of Patent: February 19, 2019
    Assignee: Youscript Inc.
    Inventors: Howard C. Coleman, Robert D. Patterson, Jessica Oesterheld, Rajeev V. Pany, Kristine Ashcraft
  • Patent number: 10201634
    Abstract: The present invention is directed to implants and the modification of the surface of implants using amino acid or polypeptide functionalized rosette nanotubes.
    Type: Grant
    Filed: June 23, 2014
    Date of Patent: February 12, 2019
    Assignees: Brown University, National Research Council of Canada, The Governors of the University of Alberta
    Inventors: Thomas J. Webster, Hicham Fenniri, Usha Devi Hemraz
  • Patent number: 10196668
    Abstract: Reagents, methods, and kits for assaying enzymes associated with lysosomal storage diseases MPS-I, MPS-II, MPS-IIIA, MPS-IIIB, MPS-IV A, MPS-VI, and MPS VII. In one aspect, the invention provides methods for assaying one or more enzymes associated with a lysosomal storage disease. In a first embodiment, the method includes: (a) contacting a sample with a first solution to provide a solution comprising one or more lysosomal enzymes; (b) contacting the one or more lysosomal enzymes in solution with an enzyme substrate for each lysosomal enzyme to be analyzed and incubating the substrates with the enzymes for a time sufficient to provide a solution comprising an enzyme product for each lysosomal enzyme present in the sample.
    Type: Grant
    Filed: September 5, 2014
    Date of Patent: February 5, 2019
    Assignee: University of Washington through its Center for Commercialization
    Inventors: Michael H. Gelb, Arun Babu Kumar, Frances Hocutt, Zdenek Spacil, Mariana Natali Barcenas Rodriguez, Frantisek Turecek, C. Ronald Scott
  • Patent number: 10189892
    Abstract: A feedback control mechanism for a fermentation of yeast cells to make recombinant proteins uses a respiratory quotient measurement which adjusts the levels of oxygenation and/or fermentable sugar feed. The feedback control mechanism permits well controlled cultures that produce good amounts of product while avoiding toxic accumulation of ethanol. Additionally, recombinant proteins so produced have excellent qualitative properties, such as excellent homogeneity and proper inter-subunit assembly.
    Type: Grant
    Filed: March 17, 2014
    Date of Patent: January 29, 2019
    Inventors: Gary Lesnicki, Patricia Dianne McNeill, Franz Hartner, Mark Young
  • Patent number: 10190114
    Abstract: Systems and methods are described for isolation, separation and detection of a molecular species using a low resource device for processing of samples. Methods include isolation, separation and detection of whole cells as well as biomolecules including viruses, proteins, nucleic acids, carbohydrates and lipids.
    Type: Grant
    Filed: August 25, 2015
    Date of Patent: January 29, 2019
    Inventors: Rick Haselton, Nick Adams
  • Patent number: 10181395
    Abstract: A mass calibration kit and a calibration method for a low-mass area of a high-resolution mass spectrometer in negative ion mode. The mass calibration kit comprises semiconductor nanometer material suspension, a free fatty acid standard solution and a MALDI sample target cleaning liquid. The mass calibration method comprises: adjusting a voltage difference between a sample target of the mass spectrometer and a slit to be 20 V; dripping the semiconductor nanometer material suspension on the surface of the sample target till a solvent is completely volatilized and dried; dripping the free fatty acid standard solution on the surface of a semiconductor nanometer material till the solvent is completely volatilized and dried; and putting the sample target in the mass spectrometer for mass calibration, wherein calibration coefficients obtained after the instrument calibration can be used for correcting a sample mass spectrometric detection result.
    Type: Grant
    Filed: January 15, 2016
    Date of Patent: January 15, 2019
    Inventors: Hongying Zhong, Xuemei Tang, Lulu Huang, Wenyang Zhang
  • Patent number: 10175232
    Abstract: The present disclosure relates to particular nucleotide sequences comprising of at least one stem and/or loop structure and which optionally bind fluorescent labels, for example DFHBI and a process for obtaining said sequences. The present disclosure also provides nucleic acid sensor for cAMP comprising reporter domain, communication module and target recognition domain. Further, the present disclosure provides for ratiometric sensors 10 for quantifying cAMP using the nucleic sensors of the instant invention. The instant disclosure further provides method for obtaining the sensors, method for detecting and measuring small molecules, such as cAMP using the sensors of the instant disclosure and kits thereof.
    Type: Grant
    Filed: April 15, 2014
    Date of Patent: January 8, 2019
    Assignee: National Centre For Biological Sciences (NCBS-TIFR)
    Inventors: Yamuna Krishnan, Suruchi Sharma
  • Patent number: 10172712
    Abstract: The invention is directed to a novel method for measuring contraction characteristics of engineered heart tissue constructs (16) which is based on the mechanical coupling of the construct (16) to a support element (8) which comprises or is mechanically coupled to a piezoelectric element (10). An apparatus (1) for carrying out the method of the invention is also provided.
    Type: Grant
    Filed: March 25, 2014
    Date of Patent: January 8, 2019
    Assignees: Universitätsklinikum Hamburg-Eppendorf, Technische Universität Hamburg-Harburg
    Inventors: Thomas Eschenhagen, Ingra Mannhardt, Jörg Müller, Christoph Warncke, Jördis Weiser
  • Patent number: 10174382
    Abstract: A genetic biomarker panel is provided for prognosing late onset ER+ breast cancer relapse, in a breast cancer survivor patient. Kits are also provided for measuring levels or the presence of an identified panel of genetic biomarkers. Methods are also provided for identifying a breast cancer survivor patient at a relatively high risk of suffering a breast cancer relapse within 8 years of diagnosis, and therefore suitable for treatment with an aggressive chemotherapeutic regimen. The method may also be used for identifying a breast cancer survivor patient not at high risk of suffering a breast cancer relapse within 8 years of diagnosis, and thus not suitable for treatment with an aggressive chemotherapeutic regimen. The genetic biomarker panel includes an oligonucleotide/nucleic acid sequence specific for the following genes: MKI67, SPAG5, ESPL1, PLK1, or a genetic panel for MKI67, SPAG5, ESPL1, PLK1 and PGR.
    Type: Grant
    Filed: May 13, 2015
    Date of Patent: January 8, 2019
    Assignees: University of Notre Dame, Indiana University Research and Technology Corporation
    Inventors: Steven Buechler, Sunil Badve, Yesim Gokmen-Polar
  • Patent number: 10174313
    Abstract: Provided herein are devices and methods for the micro-isolation of biological cellular material. A micro-isolation device described can comprise a photomask that protects regions of interest against DNA-destroying illumination. The micro-isolation device can further comprise photosensitive material defining access wells following illumination and subsequent developing of the photosensitive material. The micro-isolation device can further comprise a chambered microfluidic device comprising channels providing access to wells defined in photosensitive material. The micro-isolation device can comprise a chambered microfluidic device without access wells defined in photosensitive material where valves control the flow of gases or liquids through the channels of the microfluidic device.
    Type: Grant
    Filed: January 31, 2018
    Date of Patent: January 8, 2019
    Inventors: Emil P. Kartalov, Cheng-Chung Lee, Paul Predki
  • Patent number: 10147844
    Abstract: A quantum dot includes: a core including at least one first positive ion precursor and at least one negative ion precursor; a shell including at least one second positive ion precursor and at least one negative ion precursor and wrapping the core; and a ligand formed on a surface of the shell, wherein the first positive ion precursor is an n-period element and the second positive ion precursor is an (n-1)-period element, where n is an integer of 3 to 6.
    Type: Grant
    Filed: December 15, 2016
    Date of Patent: December 4, 2018
    Assignee: Samsung Display Co., Ltd.
    Inventors: Dong Chan Kim, Yoon Hyeung Cho, Yi Su Kim
  • Patent number: 10130946
    Abstract: This disclosure provides a system for detecting rare cells. The system includes a substrate, extensions extending outwardly from the substrate and arranged about a center axis of the substrate to define a channel enabling the fluid to move radially from the center axis to an outer edge of the substrate, and a functionalized graphene oxide disposed on the extension. This disclosure also provides a method for detecting rare cells using the system of this disclosure. The method includes the steps of introducing a sample of fluid containing the rare cells into the inlet of the system such that the sample of fluid flows radially from the inlet toward the outer edge of the substrate and capturing the rare cells as the rare cells interact with the functionalized graphene oxide disposed on the extensions.
    Type: Grant
    Filed: April 6, 2015
    Date of Patent: November 20, 2018
    Inventors: Sunitha Nagrath, Vasudha Murlidhar
  • Patent number: 10125388
    Abstract: An integrated sample purification system includes a housing, a sample container rack, a filter holder, and a cylindrical magnet. The sample container rack and the filter device holder are disposed in the housing. The sample container rack is configured to hold one or more sample containers, the filter device holder is configured to hold one or more filter devices. The cylindrical magnet is adjacent to and external to the sample container rack, and is rotated about a central, longitudinal axis of the magnet by an electric motor disposed in the housing to lyse cells. Molecules of interest in the lysed cells are purified using filters that bind specifically to the molecules of interest. The system is readily amenable to automation and rapid purification and analysis of molecules of interest, such as nucleic acids and proteins.
    Type: Grant
    Filed: December 1, 2015
    Date of Patent: November 13, 2018
    Inventors: Christopher G. Cooney, Rebecca Holmberg, Phillip Belgrader, Peter Qiang Qu
  • Patent number: 10124337
    Abstract: Various embodiments are described herein for controlling the self-assembly process of paramagnetic particles and the final particle cluster size using a liquid-liquid interface. The number of paramagnetic particles within a particle cluster and coating at the liquid-liquid interface may be controlled by systematically varying the strength of an applied magnetic field gradient and the interfacial tension of the liquid-liquid interface.
    Type: Grant
    Filed: February 3, 2017
    Date of Patent: November 13, 2018
    Inventors: Scott Tsai, Steven Jones, Eric Jervis
  • Patent number: 10119120
    Abstract: The present invention provides a method for inducing differentiation of pluripotent stem cells into neural precursor cells, comprising culturing the pluripotent stem cells in the presence of a small molecule BMP inhibitor, and induced neural precursor cells prepared by this method.
    Type: Grant
    Filed: August 12, 2010
    Date of Patent: November 6, 2018
    Assignee: Kyoto University
    Inventors: Jun Takahashi, Asuka Morizane
  • Patent number: 10102625
    Abstract: A method and system analyzes multi-resolution medical images. The method includes receiving one or more multi-resolution medical images of one or more tissues. Each multi-resolution medical image is divided into a plurality of segments. Thereafter, each segment of the plurality of segments of each multi-resolution medical image is processed in parallel, utilizing one or more computation nodes, wherein each computation node includes one or more Graphical Processing Units (GPUs). The results corresponding to each segment of a multi-resolution medical image are collated, and a collated report is displayed.
    Type: Grant
    Filed: August 31, 2015
    Date of Patent: October 16, 2018
    Inventors: Umesh Shanghvi, Rhushabh Goradia, Adarsh Jain
  • Patent number: 10098581
    Abstract: A system includes a microfluidic device configured to isolate one or more particles from a mixture, a flow rate matching device configured to match flow rate of the microfluidic device with flow rate of an electrical measurement device configured to measure an electrical property of the isolated particles, and an electrical measurement device configured to measure an electrical property of the isolated particles.
    Type: Grant
    Filed: January 16, 2015
    Date of Patent: October 16, 2018
    Assignee: Massachusetts Institute of Technology
    Inventors: Joel Voldman, Hao-Wei Su, Javier Lopez Prieto, Jongyoon Han, Lidan Wu
  • Patent number: 10093957
    Abstract: Provided is a method for imaging a blood sample and a kit and system for executing the method. The method includes introducing a cell suspension including red blood cells onto a base surface of a carrier having a vertical height (H) being greater than or equal to a vertical depth (h) of the cell suspension when on the base carrier, the cell suspension including a cell concentration (C) being determined by a defined function; allowing the cells in the cell suspension to settle on the base surface of the carrier to form a monolayer of cells thereon; and acquiring at least one microscope image of at least a portion of the monolayer of cells; wherein the at least one microscope image is obtained by a microscope set to Depth Of Field that is not more than 20% of the vertical height of the cell suspension settled on the base surface.
    Type: Grant
    Filed: March 29, 2016
    Date of Patent: October 9, 2018
    Inventors: Joseph Joel Pollak, Arnon Houri Yafin, Seth J. Salpeter
  • Patent number: 10091974
    Abstract: The invention relates to a method for determining, the sex of at least one embryo in an egg (12), in which the sex of the embryo is determined by means of at least one detection method which is noninvasive at least with regard to the egg (12), wherein at least one oestradiol value which characterizes the concentration of oestradiol in the egg (12) is determined by means of the noninvasive detection method and the sex is determined as a function of the oestradiol value.
    Type: Grant
    Filed: March 17, 2014
    Date of Patent: October 9, 2018
    Assignee: Siemens Aktiengesellschaft
    Inventors: Benjamin Sewiolo, Andreas Ziroff
  • Patent number: 10085963
    Abstract: Disclosed is a method of achieving optimal mammalian energy balance using forskolin on a particular physiological and developmental stage of the mammalian cellular system.
    Type: Grant
    Filed: November 10, 2015
    Date of Patent: October 2, 2018
    Inventors: Muhammed Majeed, Kalyanam Nagabhushanam, Sarang Bani, Anjali Pandey
  • Patent number: 10080787
    Abstract: Methods of capturing N-glycan linked glycomolecules including N-glycans, N-glycopeptides and N-glycoproteins are described. The methods provide substantially unbiased capture of charged and uncharged N-glycans and/or N-glycan linked glycomolecules. Binding reagents for substantially unbiased binding of N-glycans and/or N-glycan linked glycomolecules are also described.
    Type: Grant
    Filed: June 26, 2015
    Date of Patent: September 25, 2018
    Assignee: New England Biolabs, Inc.
    Inventors: Minyong Chen, Xiaofeng Shi, James C. Samuelson, Christopher H. Taron
  • Patent number: 10072095
    Abstract: Mice are provided that comprise a reduction or deletion of ADAM6 activity from an endogenous ADAM6 locus, or that lack an endogenous locus encoding a mouse ADAM6 protein, wherein the mice comprise a sequence encoding an ADAM6 or ortholog or homolog or fragment thereof that is functional in a male mouse. In one embodiment, the sequence is an ectopic ADAM6 sequence or a sequence that confers upon a male mouse the ability to generate offspring by mating. Mice and cells with genetically modified immunoglobulin heavy chain loci that comprise an ectopic nucleotide sequence encoding a mouse ADAM6 or functional fragment or homolog or ortholog thereof are also provided.
    Type: Grant
    Filed: January 20, 2015
    Date of Patent: September 11, 2018
    Assignee: Regeneron Pharmaceuticals, Inc.
    Inventors: Lynn Macdonald, Sean Stevens, Andrew J. Murphy, Margaret Karow
  • Patent number: 10060910
    Abstract: Provided is a composition comprising an analyte bound to a polymer where the analyte is less than about 2000 MW and the polymer further comprises more than one signal or first member of a second binding pair. Also provided is an assay for an analyte comprising combining a sample suspected of containing the analyte with the above-described composition and a binding agent that binds to the analyte; and detecting the signal or the first member of the second binding pair that is bound to the binding agent. Additionally provided is a multisignal labeling reagent comprising a first polymer covalently bound to (a) a reactive group or a first member of a first binding pair, and (b) more than one digoxigenin molecule.
    Type: Grant
    Filed: July 24, 2012
    Date of Patent: August 28, 2018
    Assignee: Enzo Life Sciences, Inc.
    Inventors: Jack Coleman, Maciej Szczepanik, Richard Jin
  • Patent number: 10060927
    Abstract: The current disclosure provides methods for detecting and quantitating the 6-O-methylguanine-DNA methyltransferase protein (MGMT) directly in biological samples that have been fixed in formalin by the method of Selected Reaction Monitoring/Multiple Reaction Monitoring (SRM/MRM) mass spectrometry. Such biological samples are chemically preserved and fixed with formaldehyde containing agents/fixatives and may include formalin-fixed tissue/cells, formalin-fixed/paraffin embedded (FFPE) tissue/cells, FFPE tissue blocks and cells from those blocks, and tissue culture cells that have been formalin fixed and/or paraffin embedded. A protein sample is prepared from the biological sample and the MGMT protein is quantitated in the sample using SRM/MRM mass spectrometry by quantitating one or more fragment peptides.
    Type: Grant
    Filed: May 16, 2016
    Date of Patent: August 28, 2018
    Assignee: Expression Pathology, Inc.
    Inventors: David B. Krizman, Todd Hembrough, Eunkyung An, Sheeno Thyparambil, Wei-Li Lao
  • Patent number: 10047399
    Abstract: A method of providing a prognosis or diagnosis of prostate cancer in a subject is described. The method includes obtaining a urine or prostate sample from the subject; determining the level of expression of ABCA1 in the sample; and comparing the level of expression of ABCA1 in the sample to the level of a control sample, wherein a decreased level of ABCA1 expression compared to the control indicates the subject has prostate cancer or a more severe form of prostate cancer. Methods of treating subjects identified as having prostate cancer, and kits for carrying out the method of prognosis or diagnosis are also described.
    Type: Grant
    Filed: January 6, 2014
    Date of Patent: August 14, 2018
    Inventors: Angela H. Ting, Byron H. Lee
  • Patent number: 10041114
    Abstract: A mechanism is provided for sequencing a biopolymer. The biopolymer is traversed from a first medium to a second medium. The biopolymer includes bases. As the biopolymer traverses from the first medium to the second medium, different forces are measured corresponding to each of the bases. The bases are distinguished from one another according to the different measured forces which are measured for each of the bases.
    Type: Grant
    Filed: June 10, 2016
    Date of Patent: August 7, 2018
    Inventors: Venkat K. Balagurusamy, Stanislav Polonsky
  • Patent number: 10041105
    Abstract: Embodiments described herein relate to assay methods and kits for detecting resistance of any enzyme to its inhibitor due to functional alteration of the enzyme, comprising, conducting two or more reactions with two or more reagent mixes optionally containing substrates for the enzyme. The mixes are substantially similar, except that one contains no enzyme inhibitor whereas the others contain an enzyme inhibitor being tested for resistance. The ratio of the signal from the reaction with an inhibitor to that from a reaction without an inhibitor is used to indicate whether the enzyme is resistant to the enzyme inhibitor and also determine the susceptibility or resistance of the enzyme to various inhibitors and further identify enzyme variants. Embodiments further relate to assay methods comprising only two reactions—one conducted in a mix containing the inhibitor and the other without the inhibitor. Further included are devices for conducting such assays.
    Type: Grant
    Filed: April 13, 2015
    Date of Patent: August 7, 2018
    Inventor: Xingxiang Li
  • Patent number: 10029996
    Abstract: The present invention provides a category of cyano-substituted asymmetric cyanine dyes having the following general structural Formula I and its synthesizing method. The cyano-substituted asymmetric cyanine dyes in present invention are easily synthesized and have long emission wavelength, high molar extinction coefficient, high sensitivity, good light stability, high fluorescence quantum yield after binding with nucleic acid, and low cell toxicity, which is beneficial for application as fluorescent dyes and could also be used in the field of identifying nucleic acid molecules, clinical diagnostics, and immunoassay testing etc.
    Type: Grant
    Filed: September 15, 2014
    Date of Patent: July 24, 2018
    Inventors: Xiaojun Peng, Si Zhang, Jiangli Fan, Jingyun Wang
  • Patent number: 10023637
    Abstract: Agonists to ICOS in combination with a blocking agent to a T cell inhibitory receptor (e.g., CTLA-4, PD-1, etc.) are demonstrated herein to be useful for the treatment of tumors.
    Type: Grant
    Filed: May 3, 2016
    Date of Patent: July 17, 2018
    Assignees: Board of Regents, The University of Texas System, Memorial Sloan-Kettering Cancer Center
    Inventors: James Allison, Padmanee Sharma, Sergio A. Quezada, Tihui Fu
  • Patent number: 10024845
    Abstract: The present invention relates to a screening method for determining whether or not a candidate compound is a modulator of an insect transient receptor potential V (TRPV) channel. The present invention further provides a method of insect control by applying to an insect-specific TRPV channel modulator determined by the screening method. The present invention further relates to an expression vector that includes a nucleic acid molecule coding for an insect TRPV channel. Also, the present invention relates to cell that includes the expression vector encoding a TRPV channel.
    Type: Grant
    Filed: December 19, 2014
    Date of Patent: July 17, 2018
    Inventors: Alexandre Nesterov, Ramani Kandasamy, Barbara Wedel, Vincent L Salgado
  • Patent number: 10016757
    Abstract: Sample container for holding and transferring a liquid sample and method thereof. The sample container includes an inlet configured to allow a liquid sample to enter a sample container, and an outlet configured to allow one or more droplets of the liquid sample to exit the sample container by one or more acoustic ejections respectively. The inlet and the outlet are in different locations.
    Type: Grant
    Filed: April 26, 2012
    Date of Patent: July 10, 2018
    Assignee: Labcyte Inc.
    Inventors: Richard N. Ellson, Joseph D. Olechno, Robert G. Kuimelis
  • Patent number: 10018589
    Abstract: A method for analyzing a sample using capillary electrophoresis is provided. By the method, following steps are performed. First, an original sample and an anionic group-containing compound are fixed to form a mixed sample, where the original sample contains an analysis component to be analyzed and a sub component other than the analysis component. Then, an aggregate of the sub component and the anionic group-containing compound is removed from the mixed sample. Then, electrophoresis is performed in a capillary tube with respect to a complex in which the analysis component and the anionic group-containing compound are bound to each other, while the mixed sample is continuously supplied.
    Type: Grant
    Filed: September 4, 2015
    Date of Patent: July 10, 2018
    Assignee: ARKRAY, Inc.
    Inventor: Naotsugu Onuma
  • Patent number: 10011876
    Abstract: This disclosure generally relates to a method, a system and a kit for diagnosing and treating various disease conditions using the gene expression value in a given sample obtained from a subject. This disclosure further relates to selecting the right set of genes as a portfolio of genes for a particular disease. Providing a set of appropriate portfolio of gene for studying the gene expression values and using the values as a diagnostic tool is also disclosed. The gene expression value is further used for intervention by pharmaceuticals as a prediction model for the treatment of the disease. An individual may have a personalized health card that may store the gene expression value for that particular individual. A system may store the data for portability and integrated view of the individual's medical data for effective diagnosis and treatment.
    Type: Grant
    Filed: June 21, 2011
    Date of Patent: July 3, 2018
    Inventor: Mahesh Kandula
  • Patent number: 10012641
    Abstract: Disclosed herein are cell lines transformed to express G-Protein Coupled Receptor GPCR22 and uses thereof for identifying guanosine analogs and/or other ligands to the receptor. In particular, techniques for transforming Drosophila Schneider 2 cells and human astrocytoma 1321N1 cell to express GPCR22 are disclosed as well as transformed cells lines. The transformed cell lines of the instant disclosure may be useful in identifying guanosine analogs and functional equivalents thereof.
    Type: Grant
    Filed: October 21, 2016
    Date of Patent: July 3, 2018
    Inventors: Michel P. Rathbone, Shucui Jiang, Francesco Caciagli, Renata Ciccarelli, Patrizia Ballerini, Patrizia Di Iorio, Patricia Giuliani, Iolanda D'Alimonte
  • Patent number: 10000598
    Abstract: Disclosed herein are compositions of, synthesis methods and methods of use for improved molecularly imprinted polymers useful for extracting target molecule compounds of wine.
    Type: Grant
    Filed: October 15, 2013
    Date of Patent: June 19, 2018
    Inventor: Joseph J. Belbruno
  • Patent number: 10000789
    Abstract: A sampling probe, system and analysis method is disclosed. The sampling probe in one embodiment is constructed of a silicon substrate having a first channel, a second channel, a first tapered tip with an opening, and a second tapered tip with an opening, wherein the first channel extends from the first tapered tip opening to the second tapered tip opening, and wherein the second channel extends from an inlet port to a junction with the first channel.
    Type: Grant
    Filed: December 11, 2015
    Date of Patent: June 19, 2018
    Assignee: The Board of Regents of the University of Oklahoma
    Inventors: Zhibo Yang, Renmeng Liu, Ning Pan, Wei Rao, Rachel M. Vowcicefski
  • Patent number: 9995709
    Abstract: Provided is a substrate modification method that enables improvement of reproducibility in measurement with use of capillary electrophoresis. The substrate modification method includes immobilizing, to a substrate surface, at least two types of modification groups selected from three types of modification groups that are a type of a modification group having one functional group, a type of a modification group having two to nine functional groups, and a type of a modification group having ten or more functional groups.
    Type: Grant
    Filed: October 26, 2012
    Date of Patent: June 12, 2018
    Assignee: ARKRAY, Inc.
    Inventor: Yusuke Nakayama
  • Patent number: 9983198
    Abstract: Disclosed herein are systems and methods for assessing electrically active cell cultures. Optionally, the data can be collected using a microelectrode array (MEA). For example, electrically active cells, such as cardiomyocytes, are cultured such that they are in electrical communication with at least a portion of the electrodes of a well of the MEA. The assessments derived from the disclosed methods may be used to reduce the effects of confounding variables in data obtained from an electrically active cell culture. The methods may also be used to determine a quantitative measure of arrhythmia burden. The methods may also be used to decide if a particular culture or set of data is suitable for inclusion in scientific and characterization studies. Also disclosed is a method of finding the global conduction velocity of an electrically active cell culture.
    Type: Grant
    Filed: March 21, 2016
    Date of Patent: May 29, 2018
    Assignee: Axion BioSystems, Inc.
    Inventors: Stacie Ann Chvatal, Daniel Christopher Millard, James David Ross
  • Patent number: 9981005
    Abstract: The invention relates to the finding that turnover of DISC1 (Disrupted in schizophrenia 1) is mediated by the F-box-containing protein FBXW7 (F-box/WD repeat-containing protein 7). The sequence within DISC1 that binds to FBXW7 and targets DISC1 for turnover by the ubiquitin-proteasome system is identified. The invention provides antagonists that inhibit this interaction and methods of using these antagonists to decrease DISC1 turnover, for example in treatment of neuropsychiatric disorders, as well as methods of identifying new antagonists.
    Type: Grant
    Filed: September 29, 2014
    Date of Patent: May 29, 2018
    Inventor: George Baillie
  • Patent number: 9977554
    Abstract: A touch display panel includes a substrate, a first electrode layer, and a second electrode layer. The first and second electrode layers are disposed on the substrate. The second electrode layer electrically connects to the first electrode layer and includes a plurality of sensing electrodes. Each sensing electrode includes a plurality of first conductive lines and a plurality of second conductive lines connected to each other. The first and second conductive lines are respectively arranged along first and second directions, wherein the second direction is different from the first direction. In one of two adjacent sensing electrodes, the outermost first conductive line has at least one first edge and at least one second edge connected to the first edge. The first edge corresponds to one of the second conductive lines of the other one of the two adjacent sensing electrodes, and has a curved shape.
    Type: Grant
    Filed: June 16, 2016
    Date of Patent: May 22, 2018
    Inventors: Tsung-Yu Wang, Pei-Chieh Chen, Chao-Hsiang Wang
  • Patent number: 9959370
    Abstract: A method includes receiving a plurality of design files at a computer-based system, wherein each of the design files is indicative of a respective structural design and a structural value associated with the respective structural design, causing a three-dimensional representation of at least a portion of the structural designs to be displayed to a user, causing a cost value to be displayed to the user for each of the displayed structural designs, receiving a user selection of a first structural design of the displayed structural designs, generating an additive manufacturing file indicative of the first structural design in a predefined format, wherein the predefined format enables the processing of the additive manufacturing file into a physical structure through an additive manufacturing process, and transmitting the selected first structural design in the predefined format.
    Type: Grant
    Filed: September 11, 2015
    Date of Patent: May 1, 2018
    Assignee: Caterpillar Inc.
    Inventor: Tazio S. Grivetti
  • Patent number: 9960027
    Abstract: A method and corresponding apparatus are disclosed for analysis of a peptide-containing sample. The sample is prepared by adding isotopically-labeled peptides corresponding to endogenous peptides of interest, and the prepared sample is analyzed by liquid chromatography-mass spectrometry (LCMS). Detection in a high-resolution, accurate mass (HRAM) MS1 spectrum of a precursor ion matching an isotopically-labeled peptide triggers acquisition of an MS/MS spectrum (preferably acquired in an ion trap or other fast mass analyzer) to determine if a product ion is present matching a characteristic product ion (e.g., the y1 ion) of the isotopically-labeled peptide. If the characteristic product ion is present, then a HRAM MS/MS spectrum is acquired for detection and quantitation of the corresponding endogenous peptide.
    Type: Grant
    Filed: May 25, 2016
    Date of Patent: May 1, 2018
    Assignee: Thermo Finnigan LLC
    Inventors: Graeme C. McAlister, Shannon Eliuk Dixon, Romain M. Huguet
  • Patent number: 9950073
    Abstract: Peptides that have been found to facilitate the delivery of siRNA molecules into cells and to function in siRNA mediated silencing of cellular targets are disclosed. Complexes that include one of the peptides and a cargo molecule are disclosed, wherein the peptide and the cargo molecule are coupled non-covalently. Also disclosed are methods of producing and using the peptides/complexes.
    Type: Grant
    Filed: January 17, 2017
    Date of Patent: April 24, 2018
    Assignees: Positec Power Tools (Suzhou) Co. Ltd., Pu Chen
    Inventors: Pu Chen, Mousa Jafari, Wen Xu, Baoling Chen, Ran Pan, Nedra Karunaratne
  • Patent number: 9951366
    Abstract: The invention relates to an improved cell-free protein synthesis method using a eukaryotic cell lysate in the presence of a caspase inhibitor, to a device for carrying out said method, and to the use of a caspase inhibitor for increasing the protein yield or for increasing the stability of the synthesized proteins in such a cell-free protein synthesis method using a eukaryotic cell lysate. In a preferred embodiment of the invention, the cell-free protein synthesis method is carried out as a continuous method in a device which comprises at least two compartments separated by a dialysis membrane. The translation reaction takes place in at least one first compartment, the reaction compartment, and during the translation reaction i) reactants are diffused from at least one other compartment, the supply and discharge compartment, into the reaction compartment, and ii) reaction byproducts are diffused from the reaction compartment into the supply and discharge compartment by means of the dialysis membrane.
    Type: Grant
    Filed: September 17, 2014
    Date of Patent: April 24, 2018
    Assignee: Fraunhofer-Gesellschaft zur Foerderung der angewandten Forschung e. V.
    Inventors: Stefan Kubick, Marlitt Stech, Doreen Wuestenhagen, Robert Quast
  • Patent number: 9944986
    Abstract: The present invention relates to novel methods for the prevention, treatment and diagnosis of Alzheimer's disease. In addition, the invention relates to methods for assessing an individual's susceptibility or pre-disposition to Alzheimer's disease. The methods of the present invention involve the use of therapeutic targets and diagnostic and/or predictive markers within the mTOR signalling pathway. The methods also involve screening subjects for genetic polymorphisms associated with rapamycin-sensitive genes.
    Type: Grant
    Filed: July 11, 2013
    Date of Patent: April 17, 2018
    Assignee: The University of Birmingham
    Inventor: Zsuzsanna Nagy