Measuring Or Testing Process Involving Enzymes Or Micro-organisms; Composition Or Test Strip Therefore; Processes Of Forming Such Composition Or Test Strip Patents (Class 435/4)
  • Patent number: 10996168
    Abstract: A computer-implemented method includes controlling an instrument to measure a fluorescence emission spectrum of a sample including a first peak emission wavelength and at least a second peak emission wavelength, emitted in response to an excitation wavelength and controlling the instrument to measure an absorbance obtained at the excitation wavelength of the sample. The method may include determining, using the computer, a ratio of the measurements at either the second peak emission wavelength, or a sum of measurements at a plurality of peak emission wavelengths including at least the first peak emission wavelength and the second peak emission wavelength, to the first peak emission wavelength, and calculating, using the computer, a value for a quality parameter based on a combination of at least the ratio and the absorbance measurement. The method may include controlling an associated process based on the quality parameter.
    Type: Grant
    Filed: May 4, 2017
    Date of Patent: May 4, 2021
    Assignee: Horiba Instruments Incorporated
    Inventor: Adam Matthew Gilmore
  • Patent number: 10988759
    Abstract: The present invention relates to methods and compositions for the high throughput screening of protein-protein interactions in yeast liquid culture. Protein fusions non-native to yeast may be expressed to replace endogenous sexual agglutination proteins and mediate library-by-library interrogation of protein interactions. The methods and compositions of the invention can be utilized for the characterization of protein interaction networks in high throughput for both binding affinity and specificity, which is crucial for understanding cellular functions, screening therapeutic candidates, and evaluating engineered protein networks.
    Type: Grant
    Filed: April 23, 2020
    Date of Patent: April 27, 2021
    Assignee: UNIVERSITY OF WASHINGTON
    Inventors: David Baker, David Younger, Eric Klavins
  • Patent number: 10987670
    Abstract: A device includes a microfluidic trap disposed along a microfluidic channel, the trap and channel having dimensions to create a fluid vortex within the trap to trap a particle of interest and an electrode having interdigitated electrically isolated fingers positioned in the trap to create an electric field across the trap such that the electric field causes electroporation of a molecule into the particle of interest. A further device includes an array of channels, traps and interdigitated electrically isolated fingers.
    Type: Grant
    Filed: April 14, 2016
    Date of Patent: April 27, 2021
    Assignee: President and Fellows of Harvard College
    Inventors: Soojung Claire Hur, Mengxing Ouyang
  • Patent number: 10967377
    Abstract: A multi-well plate allows for improvement in accuracy of experiments or tests. The present invention relates to a multi-well plate comprising: a top portion placed in a horizontal direction; a bottom portion opposing the top portion in a direction crossing the horizontal direction; and wells each recessed from the top portion toward the bottom portion, wherein the wells are horizontally arranged in a matrix with rows and columns, and each well has an opening formed at the top portion, and a peripheral wall extending from a periphery of the opening to the bottom portion. In the multi-well plate, at least two wells respectively include marks each containing at least one symbol, and each mark is placed at an opening peripheral wall end portion close to the corresponding opening.
    Type: Grant
    Filed: November 11, 2016
    Date of Patent: April 6, 2021
    Assignee: NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCE AND TECHNOLOGY
    Inventor: Yoshio Kato
  • Patent number: 10954310
    Abstract: Genetically modified mice and methods for making an using them are provided, wherein the mice comprise a replacement of all or substantially all immunoglobulin heavy chain V gene segments, D gene segments, and J gene segments with at least one light chain V gene segment and at least one light chain J gene segment. Mice that make binding proteins that comprise a light chain variable domain operably linked to a heavy chain constant region are provided. Binding proteins that contain an immunoglobulin light chain variable domain, including a somatically hypermutated light chain variable domain, fused with a heavy chain constant region, are provided. Modified cells, embryos, and mice that encode sequences for making the binding proteins are provided.
    Type: Grant
    Filed: April 25, 2017
    Date of Patent: March 23, 2021
    Assignee: Regeneran Pharmaceuticals, Inc.
    Inventors: Lynn Macdonald, Sean Stevens, Cagan Gurer, Karolina A. Meagher, Andrew J. Murphy
  • Patent number: 10953401
    Abstract: A deterministic lateral displacement array that includes a channel, within a substrate, having a first sidewall, a second sidewall, and a channel length. A condenser portion that includes an entry port and an exit port. A first array of pillars is disposed between the entry port and the exit port of the condenser portion along the channel length, the first array of pillars operative to drive a first material particle and a second material particle towards the first sidewall of the channel. A separator portion that includes an entry port and an exit port, and a second array of pillars disposed between the entry port and the exit port of the separator portion along the channel length, the pillars operative to drive the first material particle towards the second sidewall of the channel.
    Type: Grant
    Filed: May 4, 2018
    Date of Patent: March 23, 2021
    Assignee: INTERNATIONAL BUSINESS MACHINES CORPORATION
    Inventors: Huan Hu, Sung Cheol Kim, Joshua T. Smith, Benjamin H. Wunsch
  • Patent number: 10946012
    Abstract: Methods of inducing tumor regression, inhibiting tumor growth, and inducing apoptosis with selective peptidomimetic inhibitors of geranylgeranyltransferase I (GGTase I), are provided. In one aspect, GGTI-2418 and its methylester GGTI-2417, increase levels of the cyclin-dependent kinase (Cdk) inhibitor p27Kip1 and induce breast tumor regression in vivo. In another aspect, GGTI-2417 inhibits the Cdk2-mediated phosphorylation of p27Kip1 at Thr187 and accumulates p27Kip1 in the nucleus.
    Type: Grant
    Filed: July 29, 2011
    Date of Patent: March 16, 2021
    Assignees: H. Lee Moffitt Cancer Center and Research Institute, Inc., Yale University
    Inventors: Said M. Sebti, Andrew D. Hamilton
  • Patent number: 10948496
    Abstract: The invention relates to a method for identifying marker proteins for the purpose of diagnosis and risk stratification of blood coagulation disorders.
    Type: Grant
    Filed: May 8, 2016
    Date of Patent: March 16, 2021
    Assignee: LEIBNIZ-INSTITUT FÜR ANALYTISCHE WISSENSCHAFTEN—ISAS—E.V.
    Inventors: Florian Beck, Albert Sickmann, Rene Zahedi
  • Patent number: 10945762
    Abstract: A process for recovering one or more blastocysts from a uterus of a human includes a step of placing a device transvaginally into a cervical canal of the patient; a step of delivering fluid through the device to the uterus and applying a vacuum to a uterus of the patient to aspirate fluid and entrained one or more blastocysts from the uterus; and a step of performing a biopsy procedure on the one or more recovered blastocysts to remove one or more of trophectoderm cells or inner cell mass from the recovered blastocysts. The process may further include a step of storing one or more of the biopsied trophectoderm cells or inner cell mass following the biopsy procedure and a step of performing at least one molecular diagnostic assay test on the one or more of the biopsied trophectoderm cells or inner cell mass.
    Type: Grant
    Filed: October 31, 2018
    Date of Patent: March 16, 2021
    Assignee: Previvo Genetics, Inc.
    Inventors: Sandra Ann Carson, John E. Buster, Moses Cesario, Steven Paul Woodard
  • Patent number: 10940255
    Abstract: The present invention relates to a method for preparing a medical solution from at least one first liquid component and at least one second liquid component, wherein the first component and the second component are conveyed with a respective conveying means to obtain a mixed solution, wherein the conveying means are operated such that a modulation of the concentration of the first and second components takes place and the conductivity or a parameter of the mixed solution correlating with the conductivity is measured at a measurement point, wherein the modulation of the concentrations takes place in a desired state such that no modulation or a specific desired modulation of the measured conductivity or of the parameter correlated with the conductivity occurs. The present invention furthermore relates to an apparatus for preparing a medical solution as well as to a blood treatment device having such an apparatus.
    Type: Grant
    Filed: April 15, 2016
    Date of Patent: March 9, 2021
    Assignee: FRESENIUS MEDICAL CARE DEUTSCHLAND GMBH
    Inventor: Pascal Kopperschmidt
  • Patent number: 10940189
    Abstract: Systems and methods for purification and concentration of autologous alpha-2-macroglobulin (A2M) from whole blood are provided. Also provided are diagnostic methods for identifying sites in the synovial joints, spine, tendons or ligaments for treatment of pain, degeneration, or inflammation with autologous A2M. Methods for utilizing autologous A2M in combination with other autologous treatments (e.g. platelets and other growth factors) are provided in addition to combinations with exogenous drugs or carriers. Also provided is a method of producing recombinant A2M wild type or variants thereof where the bait region was modified to enhance the inhibition characteristics of A2M and/or to prolong the half life of the protein in joints and spine disc or epidural space.
    Type: Grant
    Filed: March 2, 2018
    Date of Patent: March 9, 2021
    Assignee: CYTONICS CORPORATION
    Inventors: Lewis Hanna, John David Laughlin, Shawn Robert Browning
  • Patent number: 10933416
    Abstract: The invention proposes a device for analyzing nucleic acid molecules (M), comprising: —a bead (20), on which one molecule can be anchored at one end, —a surface (520), on which the molecule can be anchored at the other end, —an actuator (30), adapted to cause the bead to move relative to said surface in one direction, —a sensor (50), adapted to measure a distance between the bead and the surface, the device further comprising a well (11), having an axis (X-X) extending along the direction of motion of the bead and a bottom (110) formed by said surface, said well being filled with electrically conductive solution (40), and receiving the bead, the sensor being adapted to measure an impedance of the well, depending on a distance between the bead and the surface, to determine, the distance between the bead and the surface.
    Type: Grant
    Filed: May 6, 2016
    Date of Patent: March 2, 2021
    Inventors: Vincent Croquette, David Bensimon, Jimmy Ouellet, Jean-François Allemand, Charles Andre, Gordon Hamilton
  • Patent number: 10934516
    Abstract: A method for controlling electrical power consumption for a first group of functional which can be used for operational management during operation of the bioreactor components during operation comprises, inter alia, adjusting a present power control signal for one or more of the functional components from the first group in order to optimise power consumption, when a comparison shows for the first group of functional components that the currently required total electrical power consumption is greater than a predefined total electrical power consumption, such that, for the first group of functional components, an adjusted total electrical power consumption is not greater than the predefined total electrical power consumption. A biotechnological apparatus comprises a bioreactor, a reactor vessel formed in the bioreactor and having a cultivation chamber, and a temperature control device provided with a heat pump and configured to control the temperature of the cultivation chamber.
    Type: Grant
    Filed: July 25, 2017
    Date of Patent: March 2, 2021
    Assignee: DASGIP Information and Technology GmbH
    Inventors: Matthias Arnold, Guido Ertel, Sebastian Selzer
  • Patent number: 10913069
    Abstract: An apparatus for amplifying a nucleic acid sequence in a polymerase chain reaction (PCR) is provided. The apparatus comprises a microprocessor configured to communicatively couple with a user input device and receive instructions from the user input device and implement the received instructions; an electromagnetic radiation (EMR) generator configured to generated EMR at frequencies ranging from 300 THz to 400 THz and communicatively coupled with the microprocessor; a reaction vessel, communicatively coupled with the microprocessor, to house the nucleic acid sequence, necessary reagents to the PCR, and particles comprising a transition metal material, and to receive the EMR generated by the EMR generator; and a temperature sensor communicatively coupled with the reaction vessel and the microprocessor.
    Type: Grant
    Filed: January 10, 2019
    Date of Patent: February 9, 2021
    Assignee: NATIONAL CHENG KUNG UNIVERSITY
    Inventors: Dar-Bin Shieh, Chih-Chia Huang, Chen-Min Chang, Tsung-Ju Li, Po-Yang Chang, Ming-Chi Hsieh
  • Patent number: 10915863
    Abstract: A method of managing an allocation of medical examinations. The method comprises documenting, in at least one dataset, for each one of a plurality of individuals of a certain population, a plurality of historical medical test results, calculating, using a computerized processor, a plurality of relative scores each indicative of a risk of having a medical condition for one of the plurality of individuals based on respective the plurality of historical medical test results, each one of the plurality of relative scores is relative to the other of the plurality of relative scores, providing a population examination framework defining at least one criterion for selecting an individual, from the plurality of individuals, for a diagnosis of the medical condition, selecting a subgroup of the plurality of individuals according to the population examination framework, and designating members of the subgroup to perform the medical examinations.
    Type: Grant
    Filed: June 19, 2014
    Date of Patent: February 9, 2021
    Assignee: Medial Research Ltd.
    Inventors: Offer Fabian, Ori Geva
  • Patent number: 10890750
    Abstract: An observation system includes a microscope optical system, an image pickup unit, an image pickup control unit, a detection unit, and an observation control unit. The image pickup unit is configured to take an image of a field-of-view range of the microscope optical system. The image pickup control unit is configured to cause the image pickup unit to take images of an observation sample in the field-of-view range at a plurality of focal positions and generate detection images. The detection unit is configured to detect a three-dimensional position of an observation target object in the observation sample from the detection images. The observation control unit is configured to fit the field-of-view range of the microscope optical system to the three-dimensional position.
    Type: Grant
    Filed: August 23, 2017
    Date of Patent: January 12, 2021
    Assignees: Sony Corporation, Kyoto University
    Inventors: Suguru Dowaki, Shiori Oshima, Eriko Matsui, Tatsutoshi Nakahata, Megumu Saito, Akira Niwa
  • Patent number: 10890577
    Abstract: Methods of determining whether specific drugs or patients carry an increased risk of causing or developing, respectively, long QT syndrome or Torsades de Pointes and methods of treating such patients.
    Type: Grant
    Filed: May 19, 2017
    Date of Patent: January 12, 2021
    Assignee: TABULA RASA HEALTHCARE, INC.
    Inventors: Jacques Turgeon, Lauren E. Steffen, Gabriel Badea, Veronique Michaud
  • Patent number: 10886008
    Abstract: The present disclosure provides systems, media, and methods for applying machine learning to determine the biological age of a sample. A method of using a biological age predictor may comprise: (a) providing data of a sample to the biological age predictor; (b) treating the sample with a substance, thereby generating a treated sample; and (c) providing data of the treated sample to the biological age predictor. The biological age predictor may generate the first biological age and/or the second biological age.
    Type: Grant
    Filed: January 23, 2019
    Date of Patent: January 5, 2021
    Assignee: Spring Discovery, Inc.
    Inventors: Ben Kamens, Ben Komalo, Wendy Cousin, Christian Elabd, Charlie Marsh, Lauren Nicolaisen
  • Patent number: 10883985
    Abstract: Disclosed is an immunochromatographic test strip by which the influences by the interfering substances in the immunochromatography method, contained in test samples, are reduced, so that it enables to accurately and specifically measure a test substance in a test sample irrespective of the amount of the test sample supplied to the assay, and to provide an immunochromatography method using the immunochromatographic test strip. The immunochromatographic test strip includes, in the order from upstream, a sample pad, a labeled substance region, a detection region and an absorption band, wherein a polymer(s) in which hydrophobic cyclic monomer(s) having an ionic functional group(s) is/are polymerized is impregnated at a region(s) upstream of the labeled substance region.
    Type: Grant
    Filed: May 20, 2016
    Date of Patent: January 5, 2021
    Assignee: DENKA SEIKEN CO., LTD.
    Inventors: Risa Kohiyama, Osamu Ishikawa, Yuki Shinohara, Takashi Miyazawa
  • Patent number: 10885392
    Abstract: A system and method are provided which use a machine learning algorithm to obtain a learned annotation of objects in one or more scales of a multiscale image. A viewing window (300) is provided for viewing the multiscale image. The viewing window is configurable on the basis of a magnification factor, which selects one of the plurality of scales for viewing, and a spatial offset parameter. A user may provide a manual annotation of an object in the viewing window, which is then used as training feedback in the learning of the machine learning algorithm. To enable the user to more effectively provide the manual annotation, the magnification factor and the spatial offset parameter for the viewing window may be automatically determined, namely by the system and method determining where in the multiscale image the manual annotation of the object would have sufficient influence on the learned annotation provided by the machine learning algorithm.
    Type: Grant
    Filed: December 5, 2017
    Date of Patent: January 5, 2021
    Assignee: KONINKLIJKE PHILIPS N.V.
    Inventors: Dmitry Nikolayevich Znamenskiy, Kamana Sigdel, Marc Van Driel
  • Patent number: 10876964
    Abstract: The invention relates to a substance determining apparatus and method for determining a substance within a fluid. Particles attach to the substance and bind to a binding surface (30), wherein a particle release curve being indicative of a release of bound particles from the binding surface (30) is determined, and a part of the particle release curve caused by particles bound to the binding surface via a predefined kind of binding is determined based on the temporal behaviour of the particle release curve. The substance within the fluid is determined based on the part of the particle release curve. The substance can therefore be determined based on particles which are bound to the binding surface via a certain kind of binding, i.e. other kinds of binding substantially do not affect the determination of the substance, thereby improving the accuracy of determining the substance.
    Type: Grant
    Filed: December 10, 2010
    Date of Patent: December 29, 2020
    Assignee: Koninklijke Philips N.V.
    Inventors: Joannes Baptist Adrianus Dionisius Van Zon, Toon Hendrik Evers, Maatje Koets
  • Patent number: 10877040
    Abstract: The present invention is directed to a mass spectrometry approach to identifying non-Spitzoid melanoma, and distinguishing non-Spitzoid nevi from non-Spitzoid malignant melanoma.
    Type: Grant
    Filed: April 29, 2019
    Date of Patent: December 29, 2020
    Assignee: FRONTIER DIAGNOSTICS, LLC
    Inventors: Jeremy L. Norris, Richard M. Caprioli, Jason B. Robbins
  • Patent number: 10874301
    Abstract: Provided are a Raman signal measuring method and apparatus which use a difference in a time scale between Raman scattered light and fluorescence. Thus, after exciting light is incident upon a target object, light scattered from the target object may be detected before the target object generates fluorescence in response to the exciting light. As a result, a Raman signal in which background fluorescence is reduced may be obtained.
    Type: Grant
    Filed: November 16, 2016
    Date of Patent: December 29, 2020
    Assignees: SAMSUNG ELECTRONICS CO., LTD., MASSACHUSETTS INSTITUTE OF TECHNOLOGY
    Inventors: Seongho Cho, Gajendra Singh, Juneyoung Lee
  • Patent number: 10876116
    Abstract: Antisense compounds for suppressing expression of ARTD3a are disclosed, as well as pharmaceutical compositions containing same and methods of producing and using same. The antisense compounds can be used to treat inflammatory disorders and conditions related to interferon-alpha production.
    Type: Grant
    Filed: July 14, 2017
    Date of Patent: December 29, 2020
    Assignee: The Board of Regents of the University of Oklahoma
    Inventors: Carol F. Webb, Julie Ward, Michelle Ratliff
  • Patent number: 10870883
    Abstract: Provided herein are Mycobacterium smegmatis porin nanopores, systems that comprise these nanopores, and methods of using and making these nanopores. Such nanopores may be wild-type MspA porins, mutant MspA porins, wild-type MspA paralog porins, wild-type MspA homolog porins, mutant MspA paralog porins, mutant MspA homolog porins, or single-chain Msp porins. Also provided are bacterial strains capable of inducible Msp porin expression.
    Type: Grant
    Filed: June 1, 2018
    Date of Patent: December 22, 2020
    Assignees: University of Washington, The UAB Research Foundation
    Inventors: Jens H. Gundlach, Michael Niederweis, Thomas Z. Butler, Mikhail Pavlenok, Mark A. Troll, Suja Sukumaran
  • Patent number: 10870826
    Abstract: A modular incubator comprising a housing structure divided into cells and having several incubator modules, each of which can be housed, in a removable manner, in a relative cell and is provided with an incubation chamber to incubate microbiological or cellular cultures. Each cell has a first group of connectors and each incubator module has a second group of connectors, each of which can be coupled to a corresponding connector of the first group of connectors of the relative cell when the incubator module is housed in the cell. The first group of connectors has at least one pneumatic connector communicating with at least one source of aeriform substance, and the second group of connectors has a corresponding pneumatic connector communicating with the relative incubation chamber to allow the aeriform substance to be introduced into the incubation chamber.
    Type: Grant
    Filed: April 22, 2016
    Date of Patent: December 22, 2020
    Inventors: Massimiliano Cesarini, Filippo Galassi, Alessia Zanelli
  • Patent number: 10866193
    Abstract: A multiple analyte detection system includes a carrier having reagents disposed thereat, with each of the reagents capable of optically changing in response to exposure to a respective analyte. The system further includes a photodetector positioned to collectively detect light interacted with each of the reagents, a processor to determine a presence or an absence of each of the analytes in response to the light collectively-detected, and an indicator to provide an indication of the presence or the absence of each of the analytes. A method of detecting multiple analytes includes exposing reagents capable of optically changing in response to exposure to a respective analyte to a sample. The method further includes collectively detecting light interacted with each of the reagents, determining a presence or an absence of each of the analytes in response to the light collectively detected, and indicating the presence or the absence of each of the analytes determined.
    Type: Grant
    Filed: April 16, 2018
    Date of Patent: December 15, 2020
    Assignee: ALVERIX, INC.
    Inventors: Ian Hardcastle, Carol T. Schembri, John Francis Petrilla, Rene B. Helbing, Daniel B. Roitman
  • Patent number: 10859567
    Abstract: The non-invasive method of assessing the reducing potential of culture medium of in vitro cultured embryos as part of an ART procedure serves as an alternative or supportive method to assess the viability of in vitro cultured embryos without doing any harm to the embryos.
    Type: Grant
    Filed: July 20, 2015
    Date of Patent: December 8, 2020
    Assignee: Pécsi Tudományegyetem
    Inventors: Gergely Montskó, Gábor Kovács, József Bódis
  • Patent number: 10837440
    Abstract: A delivery system for a sensor chip includes a plurality of selectable ports and a two-way pump port selectively connectable to each of the selectable ports. The two-way pump port is configured to allow material to be drawn or delivered from or to the two-way pump port. The delivery system also includes a chamber and a bypass waste channel that is selectively connectable to the two-way pump port. The plurality of selectable ports includes a selectable chamber port connected to the chamber and the chamber has a chamber waste exit. Material may selectively flow through the chamber to a waste collection via the chamber waste exit or flow to the waste collection via the bypass waste channel that bypasses the chamber waste exit.
    Type: Grant
    Filed: April 17, 2018
    Date of Patent: November 17, 2020
    Assignee: Roche Sequencing Solutions, Inc.
    Inventor: Robert A. Yuan
  • Patent number: 10835899
    Abstract: A deterministic lateral displacement array that includes a channel, within a substrate, having a first sidewall, a second sidewall, and a channel length. A condenser portion that includes an entry port and an exit port. A first array of pillars is disposed between the entry port and the exit port of the condenser portion along the channel length, the first array of pillars operative to drive a first material particle and a second material particle towards the first sidewall of the channel. A separator portion that includes an entry port and an exit port, and a second array of pillars disposed between the entry port and the exit port of the separator portion along the channel length, the pillars operative to drive the first material particle towards the second sidewall of the channel.
    Type: Grant
    Filed: May 4, 2018
    Date of Patent: November 17, 2020
    Assignee: INTERNATIONAL BUSINESS MACHINES CORPORATION
    Inventors: Huan Hu, Sung Cheol Kim, Joshua T. Smith, Benjamin H. Wunsch
  • Patent number: 10837972
    Abstract: The invention relates to the detection of methylmalonic acid (MMA). In a particular aspect, the invention relates to methods for detecting derivatized methylmalonic acid (MMA) by mass spectrometry.
    Type: Grant
    Filed: June 8, 2011
    Date of Patent: November 17, 2020
    Assignee: Quest Diagnostics Investments Incorporated
    Inventors: Scott M. Goldman, Julie A. Neidich, Denise Salazar, Thomas Lynn
  • Patent number: 10813921
    Abstract: The present invention provides methods to determine whether a patient with a lysosomal storage disorder will benefit from treatment with a specific pharmacological chaperone. The present invention exemplifies an in vitro method for determining ?-galactosidase A responsiveness to a pharmacological chaperone such as 1-deoxygalactonojirimycin in a cell line expressing a mutant from of ?-galactosidase A. The invention also provides a method for diagnosing Fabry disease in patients suspected of having Fabry disease.
    Type: Grant
    Filed: July 26, 2018
    Date of Patent: October 27, 2020
    Assignee: Amicus Therapeutics, Inc.
    Inventors: Elfrida Benjamin, Hung V. Do, Xiaoyang Wu, John Flanagan, Brandon Alan Wustman
  • Patent number: 10788424
    Abstract: A system for quantifying fluorescence elicited on a substance or sample. The system has an excitation light source configured to direct light to an waveguide network. The network has a probe tip adapted to transmit excitation light to the sample and receive fluorescence light elicited from the sample. A detector is configured to receive fluorescence light from the waveguide network and output a signal corresponding to the fluorescence light power, and a control system including a memory component configured to store signal to noise ratio threshold values.
    Type: Grant
    Filed: February 12, 2016
    Date of Patent: September 29, 2020
    Assignee: Auckland Uniservices Limited
    Inventor: Frederique Vanholsbeeck
  • Patent number: 10781475
    Abstract: A technique relates to separation of a mixture. A nano-deterministic lateral displacement (nanoDLD) array is configured to separate the mixture in a fluid. A feedback system is configured to control a velocity of the fluid through the nanoDLD array. The feedback system is configured to control the velocity of the fluid to separate one or more entities in the mixture.
    Type: Grant
    Filed: January 22, 2019
    Date of Patent: September 22, 2020
    Assignee: INTERNATIONAL BUSINESS MACHINES CORPORATION
    Inventors: Stacey M. Gifford, Joshua T. Smith, Benjamin H. Wunsch
  • Patent number: 10774143
    Abstract: Methods of modulating immune responses are provided, including contacting SLAT or IP3R1 with an agent that modulates binding of SLAT to IP3R1. Methods of modulating activation or differentiation of CD4+ T cells are also provided, including contacting SLAT or IP3R1 with an agent that modulates binding of SLAT to IP3R1. Peptides and fragments of an IP3R1 amino acid sequence that binds to a SLAT amino acid sequence, and peptides and fragments of a SLAT amino acid sequence that binds to am IP3R1 amino acid sequence, are further provided.
    Type: Grant
    Filed: May 1, 2015
    Date of Patent: September 15, 2020
    Assignee: LA JOLLA INSTITUTE FOR ALLERGY AND IMMUNOLOGY
    Inventor: Amnon Altman
  • Patent number: 10767160
    Abstract: The invention is in the field of cell culture. Particularly the invention relates to methods of culturing a host cell expressing a recombinant protein in a cell culture medium comprising an effective amount of a triazine dye or supplemented with an effective amount of a triazine dye, whereby production of said protein is increased relative to cells grown without said triazine dye or any other inducer.
    Type: Grant
    Filed: July 7, 2016
    Date of Patent: September 8, 2020
    Assignee: ARES TRADING S.A.
    Inventors: Anais Muhr, David Bruhlmann, Martin Jordan, Herve Broly, Matthieu Stettler
  • Patent number: 10768080
    Abstract: Methods for isolating viable cancer cells from a sample that comprises a mixture of cancerous cells and normal (non-cancerous) cells are provided. In the methods, a fluid preparation comprising a mixture of cancerous and normal cells is repeatedly exposed to fluid shear stresses, whereby the repeated exposure to the fluid shear stresses preferentially imparts fluid shear stress-resistance to the cancerous cells.
    Type: Grant
    Filed: April 2, 2018
    Date of Patent: September 8, 2020
    Assignee: University of Iowa Research Foundation
    Inventors: Michael D. Henry, J. Matthew Barnes
  • Patent number: 10761081
    Abstract: A system for diagnosing metastasis in a metastatic-suspicious sample, including: a bio-chip, an electrical signal extraction board and a processor. The bio-chip includes a biosensor including a substrate, an array of electrodes, where the array of electrodes is patterned on the substrate and at least one Human Umbilical Vein Endothelial Cell (HUVEC) which is adhered on the substrate and on the array of electrodes forming a biological trap for a metastatic cell. A metastatic-suspicious sample may be introduced onto the bio-chip allowing for diagnosis of metastasis by monitoring the time-lapse electrical signals that are recorded by the processor.
    Type: Grant
    Filed: June 7, 2017
    Date of Patent: September 1, 2020
    Inventors: Mohammad Abdolahad, Mohammad Saeid Nik Shoar, Milad Gharooni, Mohammadali Khayamian, Farshad Rezakhanloo, Saeed Ansarian
  • Patent number: 10758487
    Abstract: Compositions and methods comprising asymmetrical artificial antigen presenting cells (aAPCs) are disclosed. The non-spherical aAPCs more closely mimic endogenous cell-cell interactions and can be used for antigen-specific immunotherapy.
    Type: Grant
    Filed: December 10, 2012
    Date of Patent: September 1, 2020
    Assignee: THE JOHNS HOPKINS UNIVERSITY
    Inventors: Jordan J. Green, Joel C. Sunshine, Karlo Perica, Jonathan Schneck
  • Patent number: 10755393
    Abstract: An image normalization method and an image processing device are provided. The image processing method includes: obtaining an input image; performing a cell visualization operation on each pixel of the input image, wherein the cell visualization operation generates an output value of each pixel of the input image according to an input value of each pixel of the input image, a color distribution center corresponding to at least one cell, a contrast adjustment value, and a brightness adjustment value.
    Type: Grant
    Filed: February 26, 2019
    Date of Patent: August 25, 2020
    Assignee: Acer Incorporated
    Inventor: Hsien-Yang Li
  • Patent number: 10746724
    Abstract: In the various illustrative embodiments herein, test devices are described with opposing sensor arrays and same side contacts.
    Type: Grant
    Filed: July 25, 2016
    Date of Patent: August 18, 2020
    Assignee: Siemens Healthcare Diagnostics Inc.
    Inventors: Jennifer A. Samproni, Todd Andrade, Marlene Shi
  • Patent number: 10745703
    Abstract: A method of one or more of, inhibiting motility, migration, dispersal, and metastasis of a cell that expresses an RPTP, which is proteolytically cleaved to form intracellular fragments in the cell includes administering to the cell an amount of an agent effective to inhibit one or more of the catalytic activity and function of the proteolytically cleaved intracellular domain-containing fragments of the RPTP.
    Type: Grant
    Filed: October 2, 2015
    Date of Patent: August 18, 2020
    Assignee: CASE WESTERN RESERVE UNIVERSITY
    Inventor: Susann Brady-Kalnay
  • Patent number: 10745735
    Abstract: A method of selectively identifying live microorganisms in a sample involves contacting a sample suspected of containing a live microorganism of interest with a biomolecular precursor labeled with a reactive chemical label. The live microorganism of interest is grown under conditions that promote selective growth of the microorganism to permit the microorganism to utilize the labeled biomolecular precursor to synthesize labeled biomolecules on a cell surface of the live microorganism. Labeled live microorganisms are contacted with a reporter and/or capture element bearing a functional group that reacts with the reactive chemical label. The labeled live microorganism is analyzed to identify it. The method can specifically isolate live microorganisms from dead ones thereby reducing the possibility of false identification of contaminating microorganisms and is able to detect specific strains using tailored metabolites that are specific for a given microorganism.
    Type: Grant
    Filed: November 25, 2014
    Date of Patent: August 18, 2020
    Inventors: John Pezacki, Allison Sherratt, Yanouchka Rouleau
  • Patent number: 10736537
    Abstract: A portion of a concentric bipolar microelectrode sensor is attached to an inflatable balloon of a catheter. Another portion of the concentric bipolar microelectrode sensor is also attached to a body of the catheter. The inflatable balloon is guided to become in proximity of a tissue. The inflatable balloon is then inflated. The inflation increases the likelihood of contact between microelectrodes of the concentric bipolar microelectrode sensor with the tissue. A voltage is supplied to the microelectrodes. The tissue's impedance is accordingly measured over a frequency range. A disease of the tissue, such as a lesion, is diagnosed based on the measured impedance.
    Type: Grant
    Filed: December 28, 2015
    Date of Patent: August 11, 2020
    Assignees: CALIFORNIA INSTITUTE OF TECHNOLOGY, UNIVERSITY OF CALIFORNIA LOS ANGELES, UNIVERSITY OF SOUTHERN CALIFORNIA
    Inventors: Yu-Chong Tai, Tzung Hsiai, Yu Zhao, Xiaoxiao Zhang, Fei Yu
  • Patent number: 10739357
    Abstract: Systems and methods are provided for determining the age of cellular hemoglobin in individual red blood cells in a blood sample by determining the percentage of HbA1c. In embodiments, a method includes measuring side scatter and fluorescence of the individual red blood cells and identifying immature red blood cells and mature red blood cells from the side scatter and fluorescence measurement. In embodiments, data collected includes the exact number of red blood cells, the fraction limits in fluorescence and side scatter units, the mean value of each fraction in fluorescence and side scatter units, the mean FL1 values per fraction in arbitrary units and the mean side scatter values per fraction in arbitrary units. In embodiments, a method also includes deriving a HbA1c content from the measured mean fluorescence of the individual red blood cells and determining the percentage of HbA1c from the HbA1c content and the hemoglobin content of the red blood cells.
    Type: Grant
    Filed: September 11, 2015
    Date of Patent: August 11, 2020
    Assignee: Beckman Coulter, Inc.
    Inventors: Andreas Van Agthoven, Fabrice Malergue, Christophe Godefroy, Enrique Rabellino
  • Patent number: 10739338
    Abstract: The invention features shaped articles containing a structure and a hydrogel coating thereon, the hydrogel coating containing alginic acid conjugated to a polyalkylene oxide and a binding moiety. The hydrogel coating on the structure is sized and shaped to fit in a well in a microtiter plate and the coating does not cover the entire exterior of the surface. The invention further features methods of capturing targets using shaped articles and methods of preparing shaped articles.
    Type: Grant
    Filed: March 24, 2015
    Date of Patent: August 11, 2020
    Assignee: QT Holdings Corp
    Inventors: Sean H. Kevlahan, Brian D. Plouffe, Jeffrey A. Zonderman
  • Patent number: 10732174
    Abstract: Provided herein are methods of detecting responses of bio-artificial tissues to agents by performing assays using three-dimensional bio-artificial tissues. The methods are adaptable to high-throughput platforms.
    Type: Grant
    Filed: April 18, 2017
    Date of Patent: August 4, 2020
    Assignee: INVIVOSCIENCES, INC.
    Inventor: Tetsuro Wakatsuki
  • Patent number: 10730045
    Abstract: The present invention generally pertains to a system for performing droplet inflation, and methods and kits comprising the same. The system comprises at least one microfluidic channel comprising one or more droplets flowing therein, one or more fluid reservoirs, one or more inflators, one or more inflator nozzles, and at least one mechanism for disrupting an interface between a droplet and a fluid. The present invention provides for the inflation of a relatively controlled volume of fluid into a droplet resulting in an increase in the volume of the droplet relative to its volume prior to inflation and, accordingly, dilution of the concentration of species, if any, previously present and emulsified in the droplet.
    Type: Grant
    Filed: November 20, 2018
    Date of Patent: August 4, 2020
    Assignee: Bio-Rad Laboratories, Inc.
    Inventors: Tony Hung, Adnan Esmail, Sepehr Kiani
  • Patent number: 10717023
    Abstract: A device and method for the separation of a compound or compounds from impurities is described. The device comprises a tube having a mixing apparatus that mixes by convection a feedstock comprising one or more products such that the products can be bound to a resin and then contacted with various buffer solutions. At various distances along the cylindrical module, solutions (e.g., sample products to be purified, buffers, etc.) of various compositions can be sequentially added and removed. The resin particles can be retained within the module by filters or screens at the addition and exit ports. In this way, a slurry of resin particles can be continuously equilibrated, loaded with product, washed of impurities, eluted of processed product(s), stripped, re-equilibrated and recycled for re-use.
    Type: Grant
    Filed: October 11, 2017
    Date of Patent: July 21, 2020
    Inventor: Roddy Kevin Stafford
  • Patent number: 10689644
    Abstract: Embodiments of the present disclosure relate generally to the genetic analysis of intractable microbes. More particularly, the present disclosure provides materials and methods that incorporate chemical mutagenesis, phenotypic selection, suppression analysis, and genomic sequencing-based mutational mapping, to identify novel genetic regulators in previously intractable organisms, such as microbes that constitute the human microbiome. Given the paucity of experimental tools to manipulate bacterial genomes, there is a need for improved methods for determining how microbial communities assemble and influence human and environmental health.
    Type: Grant
    Filed: March 17, 2017
    Date of Patent: June 23, 2020
    Assignee: Duke University
    Inventors: Raphael Valdivia, Sena Bae