Preparing Organic Compound Containing A Metal Or Atom Other Than H, N, C, O, Or Halogen Patents (Class 435/131)
  • Patent number: 11261450
    Abstract: Provided is a recombinant cell that produces isoprene or terpene, wherein the recombinant cell includes an ability to synthesize isopentenyl diphosphate through a mevalonate pathway (MVA pathway), wherein the recombinant cell lacks an ability to synthesize isopentenyl diphosphate through an endogenous non-mevalonate pathway (MEP pathway), wherein the recombinant cell includes an isoprene synthase gene or a terpene synthase gene as a foreign gene, and wherein the recombinant cell produces, with the expression of the foreign gene, isoprene or terpene having 10, 15, 20, 30, or 40 carbon atoms. The mevalonate pathway is preferably an exogenous mevalonate pathway.
    Type: Grant
    Filed: February 14, 2018
    Date of Patent: March 1, 2022
    Assignee: SEKISUI CHEMICAL CO., LTD.
    Inventors: Kana Matsushima, Masahiro Furutani, Kazufumi Kawabata
  • Patent number: 11174351
    Abstract: Segmented silicone polymers incorporating segments formed from medium-chain fatty acids are generally disclosed herein. Methods of using such compounds, for example, as surfactants, are also disclosed herein, as well as methods of making such compounds, for example, from medium-chain fatty acids derived from natural oils.
    Type: Grant
    Filed: September 17, 2019
    Date of Patent: November 16, 2021
    Assignee: Wilmar Trading Pte. Ltd.
    Inventors: Paul A. Bertin, Charles Coburn
  • Patent number: 10308919
    Abstract: The present invention provides a recombinant strain, construction method thereof and a method for producing acid phosphatase using the recombinant strain. In the invention, the phosphatase gene is obtained from Pseudomonas aeruginosa by a molecular biology method, the constructed expression plasmid is transformed into E. coli BL21 (DE3). The purified enzyme and whole cells were used for the conversion of ascorbic acid to ascorbic acid-2-phosphate. Ascorbic acid-2-phosphate can be efficiently produced by controlling the ratio of substrates. When the conversion reaction is performed at pH 4.5 under 40° C. for 8 h, the output of ascorbic acid-2-phosphate reaches 54.8 g/L, the conversion is 42.9% and the space time yield is 6.9 g/L/h.
    Type: Grant
    Filed: December 21, 2016
    Date of Patent: June 4, 2019
    Assignee: JIANGNAN UNIVERSITY
    Inventors: Liming Liu, Wei Song, Jing Wu, Kai Zheng, Xiulai Chen
  • Patent number: 10301203
    Abstract: The present invention provides efficient, economical, and environmentally-friendly compositions and methods for removing pollutants from water sources. In particular embodiments, the present invention provides compositions and methods for catalyzing the degradation of pharmaceutical pollutants in wastewater using the enzyme chloroperoxidase (CPO). Another embodiment provides a method of degrading pollutants in wastewater and other water sources. In specific embodiments, the claimed composition and method can be used to degrade pharmaceutical pollutants selected from the group consisting of: acetaminophen, carbamazepine, sulfamethazine, diclofenac, and naproxen.
    Type: Grant
    Filed: March 6, 2018
    Date of Patent: May 28, 2019
    Assignee: THE FLORIDA INTERNATIONAL UNIVERSITY BOARD OF TRUSTEES
    Inventors: Xiaotang Wang, Qinghao He
  • Patent number: 10059967
    Abstract: The invention provides non-naturally occurring microbial organisms having a (2-hydroxy-3-methyl-4-oxobutoxy) phosphonate (2H3M40P) pathway, p-toluate pathway, and/or terephthalate pathway. The invention additionally provides methods of using such organisms to produce 2H3M40P, p-toluate or terephthalate. Also provided herein are processes for isolating bio-based aromatic carboxylic acid, in particular, p-toluic acid or terephthalic acid, from a culture medium, wherein the processes involve contacting the culture medium with sufficient carbon dioxide (CO2) to lower the pH of the culture medium to produce a precipitate comprised of the aromatic carboxylic acid.
    Type: Grant
    Filed: January 18, 2013
    Date of Patent: August 28, 2018
    Assignee: Genomatica, Inc.
    Inventors: Robin E. Osterhout, Anthony P. Burgard, Mark J. Burk
  • Patent number: 10017537
    Abstract: Provided is a peptide including X2SX1AAX2X3P (SEQ ID NO. 1), X2X2PX3X2AX3P (SEQ ID NO. 2), SX1AAX2X3P (SEQ ID NO. 3), or X2PX3X2AX3P (SEQ ID NO. 4), which bind to graphitic materials or volatile organic compounds.
    Type: Grant
    Filed: July 7, 2015
    Date of Patent: July 10, 2018
    Assignee: KOREA INSTITUTE OF SCIENCE AND TECHNOLOGY
    Inventors: Hyunjung Yi, Ki Young Lee, Chaun Jang, Joonyeon Chang, Sang Kyung Kim, Soomi Ju
  • Patent number: 10000743
    Abstract: The disclosure provides 2-deoxy-scyllo-inosose reductases derived from a microorganism having the ability to utilize (?)-vibo-quercitol, recombinant vectors and transformants comprising genes encoding the same, and methods of use thereof.
    Type: Grant
    Filed: December 5, 2014
    Date of Patent: June 19, 2018
    Assignee: Asahi Kasei Chemicals Corporation
    Inventors: Kazunobu Konishi, Nobuya Itoh, Junji Kurokawa
  • Publication number: 20150132809
    Abstract: The present invention provides for a system for increasing the production of a compound using an artificial positive feedback loop (APFL). In some embodiments, the system diverts a compound produced in a first metabolic pathway into a second metabolic pathway in order to produce a compound of interest.
    Type: Application
    Filed: January 27, 2015
    Publication date: May 14, 2015
    Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
    Inventor: Dominique Loque
  • Publication number: 20150118722
    Abstract: A method is described for producing lactate or lactic acid from a plant extract, such as oil palm frond extract, via fermentation. In particular, the method includes providing a fermentation medium that includes at least 25 wt. % of a plant extract containing fermentable carbohydrates, fermenting the fermentation medium by means of a lactic acid producing microorganism in the presence of a caustic magnesium salt to provide a fermentation broth containing at the most 9.5 wt. % magnesium lactate at the end of fermentation, the magnesium lactate being in soluble form during and at the end of fermentation; and recovering lactate or lactic acid from the magnesium lactate containing fermentation broth.
    Type: Application
    Filed: December 14, 2012
    Publication date: April 30, 2015
    Inventor: Peter Johannes Marie Baets
  • Patent number: 8999682
    Abstract: Disclosed are the uses of specific genes of the mevalonate and isoprenoid biosynthetic pathways, and of inactive gene sites (the pseudogene) to (1) enhance biosynthesis of isopentenyl diphosphate, dimethylallyl diphosphate and isoprenoid pathway derived products in the plastids of transgenic plants and microalgae, (2) create novel antibiotic resistant transgenic plants and microalgae, and (3) create a novel selection system and/or targeting sites for mediating the insertion of genetic material into plant and microalgae plastids. The specific polynucleotides to be used, solely or in any combination thereof, are publicly available from GeneBank and contain open reading frames having sequences that upon expression will produce active proteins with the following enzyme activities: (a) acetoacetyl CoA thiolase (EC 2.3.1.9), (b) 3-hydroxy-3-methylglutaryl-coenzyme A (HMG-CoA) synthase (EC 4.1.3.5), (c) HMG-CoA reductase (EC 1.1.1.34), (d) mevalonate kinase (EC 2.7.1.36), (e) phosphomevalonate kinase (EC 2.7.4.
    Type: Grant
    Filed: November 21, 2013
    Date of Patent: April 7, 2015
    Assignees: Danisco US Inc., Kuehnle Agrosystems Company LLC
    Inventors: Frederick M. Hahn, Adelheid R. Kuehnle
  • Patent number: 8986975
    Abstract: A method of producing sulfur-free nanoparticles involves growing yeast in a growth medium containing a source of an element in a bio-reducible oxidation state (e.g. Se(Vl), and, precipitating nanoparticles containing the element in a lower oxidation state (e.g. Se(O)) than the oxidation state of the element in the source. The method advantageously can provide substantially spherical nanoparticles at high production efficiencies.
    Type: Grant
    Filed: August 17, 2009
    Date of Patent: March 24, 2015
    Assignee: National Research Council of Canada
    Inventors: Zoltan Mester, Laurent Ouerdane
  • Patent number: 8975047
    Abstract: The present invention relates to a process for making isooctenes using dry 1-butanol derived from fermentation broth. The isooctenes so produced are useful for the production of fuel additives.
    Type: Grant
    Filed: June 13, 2007
    Date of Patent: March 10, 2015
    Assignee: E I du Pont de Nemours and Company
    Inventors: Michael B. D'Amore, Leo Ernest Manzer, Edward S. Miller, Jr., Jeffrey P. Knapp
  • Publication number: 20150004662
    Abstract: The invention provides non-naturally occurring microbial organisms having a (2-hydroxy-3-methyl-4-oxobutoxy)phosphonate pathway, p-toluate pathway, and/or terephthalate pathway. The invention additionally provides methods of using such organisms to produce (2-hydroxy-3-methyl-4-oxobutoxy)phosphonate pathway, p-toluate pathway or terephthalate pathway.
    Type: Application
    Filed: September 12, 2014
    Publication date: January 1, 2015
    Inventor: Robin E. Osterhout
  • Publication number: 20140356854
    Abstract: Methods and compositions related to a novel mevalonatephosphate decarboxylase enzymes are provided. The methods and compositions provided herein are particularly useful for the synthesis of isopentyl phosphate. The compositions and methods provided herein may also be used in combination with isopentyl phosphate kinase for the synthesis of isopentyl diphosphate.
    Type: Application
    Filed: June 4, 2014
    Publication date: December 4, 2014
    Inventors: Joseph P. Noel, Nikki M. Dellas, Gerard Manning
  • Publication number: 20140349350
    Abstract: The present invention relates to an improved process for the preparation of compound of Formula-II, which is an intermediate in the preparation of HMG-CoA reductase inhibitors. wherein X is hydrogen or hydroxy protecting group and R1 is carboxyl protecting group.
    Type: Application
    Filed: May 28, 2014
    Publication date: November 27, 2014
    Inventors: Michiel Christian Alexander Van Vliet, Willem Robert Klass Schoevaart, Madhuresh Kumar Sethi, Sanjay Mahajan, Bhairaiah Mara
  • Publication number: 20140349352
    Abstract: Provided are labdenediol diphosphate synthase polypeptides, sclareol synthase polypeptides, nucleic acid molecules encoding the labdenediol diphosphate synthase polypeptides and sclareol synthase polypeptides, and methods of using the labdenediol diphosphate synthase polypeptides, sclareol synthase polypeptides. Also provided are methods for producing labdenediol diphosphate, sclareol and (?)-ambroxide.
    Type: Application
    Filed: July 30, 2013
    Publication date: November 27, 2014
    Applicant: Allylix, Inc.
    Inventors: Grace Eunyoung Park, Bryan N. Julien, Richard Burlingame
  • Publication number: 20140342387
    Abstract: The present invention relates to enzymes capable of hydrolysing organophosphate (OP) molecules. In particular, the invention relates to variants of the OpdA enzyme from Agrobacterium that display improved activity when compared to the naturally occurring OpdA. The invention is also towards polypeptides that have organophosphate hydrolysing activity for the organophosphates chlorpyrifos methyl, diazinon and parathion ethyl.
    Type: Application
    Filed: July 20, 2012
    Publication date: November 20, 2014
    Applicant: Commonwealth Scientific and Industrial Research Organisation
    Inventors: Colin Scott, John Oakeshott, Robyn Russell, Nigel French, Steven Kotsonis, Kaiyan Liu
  • Publication number: 20140335577
    Abstract: The present invention relates to the use of polypeptides having silicase activity for the modification or synthesis of silica, silicones and other silicium (IV) compounds. The present invention also relates to the use of polypeptides having silicase activity for the modification of glass, sand, asbestos, computer chips, glass wool, fiber glass, optical fibers and silicones, for the removal of sand from oil-sands, for the removal of asbestos, and for sandblasting.
    Type: Application
    Filed: July 25, 2014
    Publication date: November 13, 2014
    Inventors: Janne Ejrnaes Toender, Martin Borchert
  • Publication number: 20140335576
    Abstract: Provided herein are methods for the increased production of intracellular acetyl-CoA, mevalonate, isoprenoid precursors, isoprene and/or isoprenoids by recombinant microorganisms via co-metabolism of substrates with varied oxidation levels.
    Type: Application
    Filed: May 15, 2014
    Publication date: November 13, 2014
    Applicants: Danisco US Inc., The Goodyear Tire & Rubber Company
    Inventors: Gopal K. CHOTANI, Alex T. NIELSEN, Dmitrii V. VAVILINE
  • Publication number: 20140329916
    Abstract: Provided herein is a non-naturally occurring microbial organism having a methanol metabolic pathway that can enhance the availability of reducing equivalents in the presence of methanol. Such reducing equivalents can be used to increase the product yield of organic compounds produced by the microbial organism, such as adipate, 6-aminocaproate, hexamethylenediamine or caprolactam. Also provided herein are methods for using such an organism to produce adipate, 6-aminocaproate, hexamethylenediamine or caprolactam.
    Type: Application
    Filed: December 16, 2013
    Publication date: November 6, 2014
    Applicant: Genomatica, Inc.
    Inventors: Anthony P. Burgard, Robin E. Osterhout, Stephen J. Van Dien, Cara Ann Tracewell, Priti Pharkya, Stefan Andrae
  • Publication number: 20140329787
    Abstract: The present invention relates to a novel Phytophthora phospholipase C and uses thereof, methods of identifying modulators and inhibitors of a biological function of the phospholipase C, and methods of inhibiting Phytophthora growth comprising inhibiting a biological function of a novel Phytophthora phospholipase C.
    Type: Application
    Filed: October 17, 2012
    Publication date: November 6, 2014
    Inventors: Dean Ian Phillips, Peter Luke Beech
  • Publication number: 20140273111
    Abstract: The invention relates to functionalized, telechelic polymers synthesized by enzymatic catalysis and methods, and the functionalization of polymers via Michael addition with a lipase catalyst, and the crosslinking of mono- or difunctional (telechelic) polymers made by enzymatic catalysis, such as by using multifunctional coupling agents and enzyme catalysts. Quantitative transesterification of vinyl methacrylate with poly(ethylene glycol), poly(isobutylene) and poly(dimethylsiloxane) was achieved using Candida antarctica lipase B. In addition, methacrylate-functionalized poly(ethylene glycol) monomethyl ether has been successfully coupled to aminoethoxy poly(ethylene glycol) monomethyl ether via Michael addition using Candida antarctica lipase B. Amine-functionalized poly(ethylene glycol)s have also been used for the preparation of poly(ethylene glycol)-based dendrimers and gels through Michael addition of the polymer onto triacryloyl hexahydro-triazine using the same enzyme.
    Type: Application
    Filed: April 3, 2014
    Publication date: September 18, 2014
    Applicant: The University of Akron
    Inventors: Judit E. Puskas, Mustafa Yasin Sen
  • Patent number: 8822188
    Abstract: The present disclosure relates to the use of polypeptides having silicase activity for the modification or synthesis of silica, silicones and other silicium (IV) compounds. The present invention also relates to the use of polypeptides having silicase activity for the modification of glass, sand, asbestos, computer chips, glass wool, fiber glass, optical fibers and silicones, for the removal of sand from oil-sands, for the removal of asbestos, and for sandblasting.
    Type: Grant
    Filed: December 18, 2009
    Date of Patent: September 2, 2014
    Assignee: Novozymes A/S
    Inventors: Janne Ejrnæs Toender, Martin Borchert
  • Patent number: 8815548
    Abstract: The invention features methods for producing isoprene from cultured cells using a feedback-resistant mevalonate kinase polypeptide, such as an archaeal mevalonate kinase polypeptide. The resulting isoprene compositions may have increased yields and/or purity of isoprene.
    Type: Grant
    Filed: December 28, 2012
    Date of Patent: August 26, 2014
    Assignees: Danisco US Inc., The Goodyear Tire & Rubber Company
    Inventors: Zachary Q. Beck, Anthony R. Calabria, Michael C. Miller, Alex T. Nielsen, Dmitrii V. Vaviline
  • Publication number: 20140212931
    Abstract: The present invention concerns iodoperoxidases from Zobellia galactanivorans, isolated nucleic acids encoding same, as well as methods for preparing these enzymes. Moreover, the invention is also directed to the use of such iodoperoxidases in a wide range of industrial, pharmaceutical, medical, cosmetics, and ecological applications, as well as in the food industry.
    Type: Application
    Filed: July 5, 2012
    Publication date: July 31, 2014
    Applicants: Universite Pierre et Marie Curie (Paris 6), Centre National de la Recherche Scientifique (CNRS)
    Inventors: Catherine Leblanc, Ludovic Delage, Gurvan Michel, Etienne Rebuffet, Mirjam Czjzek, Philippe Potin
  • Publication number: 20140171675
    Abstract: The invention relates to an isoprene oligomer that contains a trans structural moiety and a cis structural moiety, which can be represented by the following formula (1), wherein at least 1 atom or group in the trans structural moiety is replaced by another atom or group. The invention also relates to a polyisoprene, which is biosynthesized using the isoprene oligomer and isopentenyl diphosphate. Further, this invention provides a rubber composition comprising the isoprene oligomer and/or the polyisoprene, and a pneumatic tire, including tire components (e.g., treads and sidewalls) formed from the rubber composition.
    Type: Application
    Filed: June 28, 2011
    Publication date: June 19, 2014
    Applicants: YAMAGATA UNIVERSITY, SUMITOMO RUBBER INDUSTRIES, LTD.
    Inventors: Yukino Miyagi, Naoya Ichikawa, Norimasa Ohya
  • Publication number: 20140113339
    Abstract: The present invention relates to a technique for capturing carbon dioxide and converting the carbon dioxide to carbonate minerals using a recombinant whole cell biocatalyst expressing carbonic anhydrase. More particularly, the present invention relates to a composition for capturing carbon dioxide and a method for capturing carbon dioxide using the composition, which composition comprises a whole cell of a transformant formed with a vector including a nucleic acid encoding a recombinant carbonic anhydrase; a cell lysate or its fraction of the whole cell; or a recombinant carbonic anhydrase isolated from the whole cell. Further, the present invention relates to a composition and method for converting the carbon dioxide to carbonate minerals using the carbon dioxide capturing composition.
    Type: Application
    Filed: April 13, 2012
    Publication date: April 24, 2014
    Inventors: Hyung Joon Cha, Im Gyu Kim, Dong Gyun Kang, Byung Hoon Jo, Jeong Hyun Seo
  • Publication number: 20140106416
    Abstract: Disclosed are the uses of specific genes of the mevalonate and isoprenoid biosynthetic pathways, and of inactive gene sites (the pseudogene) to (1) enhance biosynthesis of isopentenyl diphosphate, dimethylallyl diphosphate and isoprenoid pathway derived products in the plastids of transgenic plants and microalgae, (2) create novel antibiotic resistant transgenic plants and microalgae, and (3) create a novel selection system and/or targeting sites for mediating the insertion of genetic material into plant and microalgae plastids. The specific polynucleotides to be used, solely or in any combination thereof, are publicly available from GeneBank and contain open reading frames having sequences that upon expression will produce active proteins with the following enzyme activities: (a) acetoacetyl CoA thiolase (EC 2.3.1.9), (b) 3-hydroxy-3-methylglutaryl-coenzyme A (HMG-CoA) synthase (EC 4.1.3.5), (c) HMG-CoA reductase (EC 1.1.1.34), (d) mevalonate kinase (EC 2.7.1.36), (e) phosphomevalonate kinase (EC 2.7.4.
    Type: Application
    Filed: November 21, 2013
    Publication date: April 17, 2014
    Applicants: Danisco US Inc., Kuehnle Agrosystems Company LLC
    Inventors: Frederick M. Hahn, Adelheid R. Kuehnle
  • Publication number: 20140073020
    Abstract: Provided are labdenediol diphosphate synthase polypeptides, sclareol synthase polypeptides, nucleic acid molecules encoding the labdenediol diphosphate synthase polypeptides and sclareol synthase polypeptides, and methods of using the labdenediol diphosphate synthase polypeptides, sclareol synthase polypeptides. Also provided are methods for producing labdenediol diphosphate, sclareol and (?)-ambroxide.
    Type: Application
    Filed: July 30, 2013
    Publication date: March 13, 2014
    Applicant: Allylix, Inc.
    Inventors: Grace Eunyoung Park, Bryan N. Julien, Richard Burlingame
  • Patent number: 8658401
    Abstract: Disclosed is a method for preparing L-?-Glycerylphosphorylcholine with high yields and purity. The method uses phospholipase A1-based enzymatic hydrolysis, ion-exchange resin purification and silica gel column chromatography to prepare L-?-glycerylphosphorylcholin with purity up to 99.8% and a final yield up to 78.4%. The method disclosed is simple, cost-effective, environmentally friendly, and adaptable to industrial applications.
    Type: Grant
    Filed: March 24, 2011
    Date of Patent: February 25, 2014
    Assignee: Jiannan University
    Inventors: Yuanfa Liu, Kangyi Zhang, Xingguo Wang, Xiangyun Qian, Li Zhou, Zhihua Song
  • Publication number: 20140017725
    Abstract: The present invention is in the fields of nanotechnology and biomimetics. In particular, the present invention relates to the use of modified ribosomes to produce biomimetic structures. These biomimetic structures, also known as directed element polymers, are not produced by traditional industrial means but instead are produced by living systems comprising modified ribosomes.
    Type: Application
    Filed: February 1, 2013
    Publication date: January 16, 2014
    Inventor: Alexander SUNGUROFF
  • Publication number: 20130302861
    Abstract: A method of producing at least one terpene in a yeast cell is disclosed. The method comprises exogenously expressing within the mitochondria of the yeast cell or directing localization thereto a terpene synthase.
    Type: Application
    Filed: May 14, 2013
    Publication date: November 14, 2013
    Applicant: Yissum Research Development Company of the Hebrew University of Jerusalem Ltd.
    Inventor: Yissum Research Development Company of the Hebrew University of Jerusalem Ltd.
  • Publication number: 20130280769
    Abstract: The invention features methods for producing isoprene from cultured cells using a feedback-resistant mevalonate kinase polypeptide, such as an archaeal mevalonate kinase polypeptide. The resulting isoprene compositions may have increased yields and/or purity of isoprene.
    Type: Application
    Filed: December 28, 2012
    Publication date: October 24, 2013
    Inventors: Zachary Q. BECK, Anthony R. CALABRIA, Michael C. MILLER, Alex T. NIELSEN, Dmitrii V. VAVILINE
  • Publication number: 20130213264
    Abstract: A method of producing a hydrated lime. The process hydrates quicklime in conjunction with standard means of hydrating lime. The resulting hydrated lime has highly reduced contents of calcium oxide and magnesium oxide. The hydrated lime has little to no remaining reactivity when placed in contact with water after the process. The hydrated lime can is with stoichiometric volumes of water as required to fully hydrate the quicklime and water mixture as well as with volumes beyond the calculated stoichiometry with some potential for remaining water left after the process without the potential for lime putty or a wet hydrate as the result.
    Type: Application
    Filed: February 16, 2012
    Publication date: August 22, 2013
    Inventors: Gregory S. Hein, Sun Yong Kim
  • Patent number: 8507286
    Abstract: Disclosed is a method of analyzing an oxidation state of a methionine residue in a protein sample, which comprises the steps of: inducing a reaction between a protein sample having a methionine residue with an oxidation state to be analyzed, and hydrogen peroxide H218O2 having oxygen atoms labeled by the isotope 18O, to obtain a modified protein sample in which the oxidation state of the methionine residue is stabilized; and subjecting the modified protein sample to a measurement to quantify an oxidation degree of the methionine residue. Preferably, the measurement is a mass spectrometric (MS) measurement using a mass spectrometer. The method can analyze an oxidation state of a methionine residue in a protein sample, in a simple manner, while accurately reflecting an in vivo oxidation state of the methionine residue.
    Type: Grant
    Filed: November 20, 2008
    Date of Patent: August 13, 2013
    Assignee: Shimadzu Corporation
    Inventor: Hirotaka Fujimoto
  • Publication number: 20130203132
    Abstract: Disclosed are the uses of specific genes of the mevalonate and isoprenoid biosynthetic pathways, and of inactive gene sites (the pseudogene) to (1) enhance biosynthesis of isopentenyl diphosphate, dimethylallyl diphosphate and isoprenoid pathway derived products in the plastids of transgenic plants and microalgae, (2) create novel antibiotic resistant transgenic plants and microalgae, and (3) create a novel selection system and/or targeting sites for mediating the insertion of genetic material into plant and microalgae plastids. The specific polynucleotides to be used, solely or in any combination thereof, are publicly available from GeneBank and contain open reading frames having sequences that upon expression will produce active proteins with the following enzyme activities: (a) acetoacetyl CoA thiolase (EC 2.3.1.9), (b) 3-hydroxy-3-methylglutaryl-coenzyme A (HMG-CoA) synthase (EC 4.1.3.5), (c) HMG-CoA reductase (EC 1.1.1.34), (d) mevalonate kinase (EC 2.7.1.36), (e) phosphomevalonate kinase (EC 2.7.4.
    Type: Application
    Filed: September 4, 2012
    Publication date: August 8, 2013
    Inventors: Frederick M. Hahn, Adelheid R. Kuehnle
  • Publication number: 20130130337
    Abstract: The invention relates to an enzymatic acylation method including at least the following steps of: contacting at least one compound having at least one function selected from among the amine, alcohol, or thiol functions, at least one microorganism having an acyl transfer activity and/or an acyl transfer enzyme, and at least one acylphosphonate donor of formula (I), where: R is an alkyl, alkene, uikyne, aryl, or aralkyl radical, or is —ORa, —SRa, —NRaRb, where Ra and Rb are identical or different and are H, an alkyl, alkene, alkyne, aryl or aralkyl radical, the alkyl, alkene, alkyne, aryl or aralkyl radicals being optionally substituted; X is O or S; Y and Z, which are identical or different, are —OR1, —OR2, —SR1, —SR2, —NR?1R?1, —NR?2R?2; R1, R2, R?1, R?2, R?1 and R?2, which are identical or different, are an alkyl, alkene, alkyne, aryl or aralkyl radical, said alkyl, alkene, alkyne, aryl or aralkyl radicals being optionally substituted; and recovering the compound including at least one acyl function, said fu
    Type: Application
    Filed: July 29, 2011
    Publication date: May 23, 2013
    Applicant: PCAS BIOSOLUTION
    Inventors: Juliette Martin, Gerard Guillamot, Laurent Fourage, Denis Wahler
  • Patent number: 8440169
    Abstract: The present invention herein provides, for instance, a stable isotope-labeled phenylalanine wherein a carbon atom of the phenyl group linked to an amino acid residue is 13C, 2 to 4 carbon atoms of the remaining 5 carbon atoms constituting the phenyl group are 12C atoms to which deuterium atoms are bonded, and the remaining carbon atoms are 13C atoms to which hydrogen atoms are linked, and a stable isotope-labeled tyrosine wherein a carbon atom of the phenyl group linked to an amino acid residue is 13C, the carbon atom bonded to the hydroxyl group (OH group) of the phenyl group is 12C or 13C, 2 to 4 carbon atoms of the remaining 4 carbon atoms constituting the phenyl group are 12C atoms to which deuterium atoms are bonded, and the remaining carbon atoms are 13C atoms to which hydrogen atoms are linked.
    Type: Grant
    Filed: September 14, 2009
    Date of Patent: May 14, 2013
    Assignee: Japan Science and Technology Agency
    Inventors: Masatsune Kainosho, Tsutomu Terauchi
  • Patent number: 8431368
    Abstract: The invention relates to a method for the enzymatic production of C6-C18 fatty alcohol and/or C8-C18 fatty acid, by carbon chain elongation, comprising the steps of: i) providing organic C2-C6 compounds; ii) subjecting the organic C2-C6 compounds to enzymatic carbon chain elongation in the presence of an electron donor; and iii) separating the formed C6-C18 fatty alcohol and/or C8-C18 fatty acid.
    Type: Grant
    Filed: December 16, 2008
    Date of Patent: April 30, 2013
    Assignee: Waste2Chemical Knowledge B.V.
    Inventors: Hubertus Victor M. Hamelers, Kirsten Johanna J. Steinbusch, Cees Jan Nico Buisman
  • Publication number: 20130052692
    Abstract: The present invention provides for a genetically modified host cell capable of producing 1-deoxyxylulose 5-phosphate or 1-deoxy-D-xylulose 5-phosphate (DXP) (12), and optionally one or more DXP derived compounds, comprising: (a) a mutant RibB, or functional variant thereof, capable of catalyzing xylulose 5-phosphate and/or ribulose 5-phosphate to DXP, or (b) a YajO, or functional variant thereof, and a XylB, or functional variant thereof.
    Type: Application
    Filed: August 16, 2012
    Publication date: February 28, 2013
    Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
    Inventors: James Kirby, Jeffrey L. Fortman, Minobu Nishimoto, Jay D. Keasling
  • Publication number: 20130045514
    Abstract: Carbonic anhydrase can be expressed on a cell surface in a system and method for mineralizing carbon dioxide. The system and method can optionally include a mineralization peptide to facilitate formation of minerals from carbonate ions and divalent metal cations.
    Type: Application
    Filed: August 17, 2011
    Publication date: February 21, 2013
    Inventors: Roberto Barbero, Elizabeth Wood, Angela Belcher
  • Patent number: 8361762
    Abstract: The invention features methods for producing isoprene from cultured cells using a feedback-resistant mevalonate kinase polypeptide, such as an archaeal mevalonate kinase polypeptide. The resulting isoprene compositions may have increased yields and/or purity of isoprene.
    Type: Grant
    Filed: September 15, 2009
    Date of Patent: January 29, 2013
    Assignees: Danisco US Inc., The Goodyear Tire & Rubber Company
    Inventors: Zachary Q. Beck, Anthony R. Calabria, Michael C. Miller, Dmitrii V. Vaviline, Alex T. Nielsen
  • Publication number: 20130018124
    Abstract: A process for making biodegradable a synthetic polymeric material thanks to the addition of one or more yeasts to the synthetic material is disclosed.
    Type: Application
    Filed: January 25, 2011
    Publication date: January 17, 2013
    Applicant: QUEENSBROOK LIMITED
    Inventor: Madrisano Catinari
  • Publication number: 20130011888
    Abstract: The invention relates to recombinant geranyl diphosphate (GPP) synthases, genes encoding the synthases, vectors and host cells comprising the same. More particularly, the invention relates to a recombinant GPP synthase, which preferentially facilitates the production of GPP from its isoprenoid precursors and incorporating a recombinant GPP synthase into one or more terpene production pathways of a host organism.
    Type: Application
    Filed: March 30, 2011
    Publication date: January 10, 2013
    Applicant: CODEXIS, INC.
    Inventors: Oscar Alvizo, Robert McDaniel, Benjamin Mijts
  • Publication number: 20130011887
    Abstract: In alternative embodiments, the invention provides phosphatidylinositol-specific phospholipase C (PI-PLC) enzymes, nucleic acids encoding them, antibodies that bind specifically to them, and methods for making and using them. Industrial methods and products comprising use of these phospholipases are also provided. In certain embodiments, provided herein are methods for hydration of non hydratable phospholipids (NHPs) within a lipid matrix. The methods enable migration of NHPs to an oil-water interface thereby allowing the NHPs to be reacted and/or removed from the lipids. In certain embodiments, provided is a method for removing NHPs, hydratable phospholipids, and lecithins from vegetable oils to produce a degummed oil or fat product that can be used for food production and/or non-food applications. In certain embodiments, provided herein are methods for hydration of NHPs followed by enzymatic treatment and removal of various phospholipids and lecithins.
    Type: Application
    Filed: October 8, 2010
    Publication date: January 10, 2013
    Inventors: Christopher L.G. Dayton, Flavio Da Silva Galhardo, Nelson Barton, Tim Hitchman, Jonathan Lyon, Eileen O'Donoghue, Mark A. Wall
  • Publication number: 20130005871
    Abstract: The present invention relates to a method for preparing an organically functionalized layered double hydroxide which comprises reacting (a) a layered double hydroxide, (b) a triglyceride oil, (c) an enzyme which is capable of catalyzing the hydrolysis of an ester, and (d) water, to produce the organically functionalized layered double hydroxide. The subject invention further reveals a method for preparing an organically functionalized layered double hydroxide as specified in claim 1 which comprises (1) dispersing into an aqueous medium (a) the layered double hydroxide, (b) the triglyceride oil, and (c) the enzyme, (2) allowing the triglyceride oil to hydrolyze into long-chain fatty acids and glycerol to produce a dispersion of the organically functionalized layered double hydroxide; and (3) recovering the organically functionalized layered double hydroxide from the aqueous dispersion.
    Type: Application
    Filed: June 20, 2012
    Publication date: January 3, 2013
    Applicant: FLOW POLYMERS LLC
    Inventors: Aaron S. Puhala, Xiaofan Luo
  • Publication number: 20120302777
    Abstract: The present invention provides a novel glycerol 3-phosphateacyltransferase gene and use thereof. The object of the present invention can be solved by providing a nucleic acid having a nucleotide sequence set forth in SEQ ID NO: 1, 4, or 8, SEQ ID NO: 3, 6, or 11, or SEQ ID NO: 7 or 12 and a mutant thereof. The present invention also provides a protein having an amino acid sequence set forth in SEQ ID NO: 2, 5, or 9 and a mutant thereof.
    Type: Application
    Filed: February 3, 2011
    Publication date: November 29, 2012
    Applicant: SUNTORY HOLDINGS LIMITED
    Inventor: Misa Ochiai
  • Patent number: 8318914
    Abstract: The present invention relates to polynucleotides from Pythium irregulare, Rhizopus oryzae and Euglena gracilis which code for elongases and which can be employed for the recombinant production of polyunsaturated fatty acids. The invention furthermore relates to vectors, host cells and transgenic nonhuman organisms which comprise the polynucleotides according to the invention, and to the polypeptides encoded by the polynucleotides. The invention furthermore relates to antibodies against the polypeptides according to the invention. Finally, the invention also relates to production processes for the polyunsaturated fatty acids and for oil, lipid and fatty acid compositions and to their use as drugs, cosmetics, foodstuffs, feedstuffs, preferably fish food, or food supplements.
    Type: Grant
    Filed: July 30, 2008
    Date of Patent: November 27, 2012
    Assignees: Bioriginal Food & Science Corp., BASF Plant Science GmbH
    Inventors: Jörg Bauer, Xiao Qiu, Patricia Vrinten
  • Publication number: 20120264960
    Abstract: The present invention provides a novel acetyl-CoA carboxylase. The object of the present invention is attained by the nucleotide sequence of SEQ ID NO: 1 and the amino acid sequence of SEQ ID NO: 2 of the present invention.
    Type: Application
    Filed: March 17, 2010
    Publication date: October 18, 2012
    Applicant: SUNTORY HOLDINGS LIMITED
    Inventors: Misa Ochiai, Eiji Sakuradani, Sakayu Shimizu, Jun Ogawa
  • Publication number: 20120225095
    Abstract: This invention provide processes for producing carboxylic acid product, along with useful salts. The carboxylic acid product that is produced according to this invention is preferably a C2-C12 carboxylic acid. Among the salts produced in the process of the invention are ammonium salts.
    Type: Application
    Filed: March 3, 2011
    Publication date: September 6, 2012
    Applicant: MICHIGAN BIOTECHNOLOGY INSTITUTE
    Inventors: Robert J. Hanchar, Susanne Kleff, Michael V. Guettler