Concentration, Separation, Or Purification Of Micro-organisms Patents (Class 435/173.9)
  • Patent number: 10456791
    Abstract: A method for performing contactless ODEP for separation of CTCs is provided with the steps of obtaining patients' blood with rare cell suspected CTCs; adding at least one fluorescent antibody binding to CTCs into the blood; staining the blood; injecting the stained blood with fluorescent dye into an ODEP device and then performing fluorescent image identification; trapping the CTCs with at least one fluorescent antibody in the ODEP device by creating an image pattern and then generating an ODEP force; Separating the trapped CTCs from other non-CTCs cells; absorbing the trapped CTCs; and obtaining a high purity of CTCs. An apparatus for performing contactless ODEP for separation of CTCs is also provided.
    Type: Grant
    Filed: December 1, 2016
    Date of Patent: October 29, 2019
    Assignee: Ace Medical Technology Co., Ltd.
    Inventors: Min-Hsien Wu, Chia-Hsun Hsieh, Hung-Ming Wang, Wen-Pin Chou, Tzu-Keng Chiu
  • Patent number: 10444125
    Abstract: Devices for separating magnetically labeled moieties in a sample are provided. Aspects of the devices include a magnetic field source, a first magnetic field guide having a wedge-shaped portion with an apex edge, and a second magnetic field guide having a wedge-shaped portion with an apex edge. The apex edge of the first magnetic field guide is aligned substantially across from and parallel to the apex edge of the second magnetic field guide, and the device is configured to separate magnetically labeled moieties from non-magnetically labeled moieties in the sample. Also provided are methods of using the devices, as well as systems and kits configured for use with the devices and methods.
    Type: Grant
    Filed: December 26, 2017
    Date of Patent: October 15, 2019
    Assignee: BECTON, DICKINSON AND COMPANY
    Inventor: Liping Yu
  • Patent number: 10416155
    Abstract: The invention generally relates to methods of using compositions that include sets of magnetic particles, members of each set being conjugated to an antibody specific for a pathogen, and magnets to isolate a pathogen from a body fluid sample.
    Type: Grant
    Filed: May 2, 2018
    Date of Patent: September 17, 2019
    Assignee: DNAE Group Holdings Limited
    Inventors: Sergey A. Dryga, Victor C. Esch, Lisa-Jo Ann Clarizia, Eddie W. Adams, Thearith H. Ung, Ravil A. Sitdikov
  • Patent number: 10094837
    Abstract: Methods of isolating, enriching, capturing, identifying, or detecting the presence of, cancerous cells in a sample, e.g., a blood sample from a subject, by detecting the presence of one or more cancer cell surface markers selected from the group consisting of cadherin 1 (CDH1), CDH2, CDH3, CDH4, CDH5, CDH9, CDH11, CDH17, CDH19, protocadherin 9 (PCDH9) and/or PCDH beta 13 (PCDHb13), and optionally an additional cancer cell surface marker, e.g., EpCAM, MUC1, EphB4, EGFR, CEA, and/or HER2.
    Type: Grant
    Filed: August 15, 2016
    Date of Patent: October 9, 2018
    Assignee: The General Hospital Corporation
    Inventors: Shyamala Maheswaran, David Tsai Ting, Daniel A. Haber
  • Patent number: 9599610
    Abstract: The invention generally relates to a system for isolating or separating a target from a sample. In certain aspects, processes performed by the target capture system include introducing a plurality of magnetic particles, in which a plurality of the particles include at least one binding moiety specific to a target, into a sample to form at least one target/particle complex and applying a magnetic field to isolate the magnetic particle/target complexes from the sample. The process starts at inputting a sample into the system and ends at delivering a capture target or nucleic acids of the target into a container for further analysis.
    Type: Grant
    Filed: December 16, 2013
    Date of Patent: March 21, 2017
    Assignee: DNAE Group Holdings Limited
    Inventors: Ravil A. Sitdikov, Eddie W. Adams, Magdalena A. Torrance, David K. Aley, Erik J. Smith, Victor C. Esch
  • Patent number: 9034579
    Abstract: The present invention includes methods, devices and systems for isolating a nucleic acid from a fluid comprising cells. In various aspects, the methods, devices and systems may allow for a rapid procedure that requires a minimal amount of material and/or results in high purity nucleic acid isolated from complex fluids such as blood or environmental samples.
    Type: Grant
    Filed: October 10, 2014
    Date of Patent: May 19, 2015
    Assignee: BIOLOGICAL DYNAMICS, INC
    Inventors: Rajaram Krishnan, David J. Charlot, Eugene Tu, James McCanna, Lucas Kumosa, Paul D. Swanson, Robert Turner, Kai Yang, Irina Dobrovolskaya, David Liu, Juan Pablo Hinestrosa Salazar, Juscilene Menezes
  • Patent number: 9034578
    Abstract: The present invention includes methods, devices and systems for isolating a nucleic acid from a fluid comprising cells. In various aspects, the methods, devices and systems may allow for a rapid procedure that requires a minimal amount of material and/or results in high purity nucleic acid isolated from complex fluids such as blood or environmental samples.
    Type: Grant
    Filed: October 7, 2014
    Date of Patent: May 19, 2015
    Assignee: BIOLOGICAL DYNAMICS, INC.
    Inventors: Rajaram Krishnan, David Charlot, Eugene Tu, James McCanna, Lucas Kumosa, Paul Swanson, Robert Turner, Kai Yang, Irina Dobrovolskaya, David Liu
  • Publication number: 20150118728
    Abstract: According to embodiments of the present invention, an apparatus for separating a biological entity from a sample volume is provided. The apparatus includes an input chamber including an inlet configured to receive the volume sample, and an outlet, at least one magnetic element adjacent a portion of the input chamber, the magnetic element configured to provide a magnetic field in a vicinity of the portion of the input chamber to trap at least some leukocytes from the sample volume, and a filter in fluid communication with the outlet, the filter configured to separate the biological entity. According to further embodiments of the present invention, a method for separating a biological entity from a sample volume is also provided.
    Type: Application
    Filed: April 19, 2013
    Publication date: April 30, 2015
    Applicant: AGENCY FOR SCIENCE, TECHNOLOGY AND RESEARCH
    Inventors: Abdur Rub Abdur Rahman, Chandran Jegatha, Lakshmi Shankar, Chee Chung Wong
  • Publication number: 20150110752
    Abstract: Methods of making and using a magnetic ECM are disclosed. The ECM comprises positively and negatively charged nanoparticles, wherein one of said nanoparticles contains a magnetically responsive element. When the magnetic ECM is seeded with cells, the cells will be magnetized and can be levitated for 3-D cell culture.
    Type: Application
    Filed: June 11, 2013
    Publication date: April 23, 2015
    Inventor: Glauco R. Souza
  • Publication number: 20150111277
    Abstract: A system is disclosed for washing a selected component and removing the selected component from a wash material. The selected component may include red blood cells that are washed to remove a rejuvenating solution. The red blood cells may then be removed with various systems, such as a standing acoustic wave system from the wash solution. Pumps and flow restrictors that provide steady flow from pumps that generate pulsed flow are also disclosed.
    Type: Application
    Filed: October 21, 2014
    Publication date: April 23, 2015
    Inventors: Ned M. HAMMAN, Michael D. LEACH, David ABESKARON
  • Publication number: 20150111276
    Abstract: The invention relates to methods of isolating white blood cells (WBCs) from a sample, e.g., whole blood, using magnetic particles that specifically bind to WBCs and a series of specific steps and conditions. The methods can include one or more of decreasing the viscosity of the sample prior to WBC isolation, agitating the sample at specified frequencies, and/or using a sample container arranged such that all of the sample is placed in close proximity (e.g., within 5, 2, 1, or 0.5 mm) to the source of the magnetic field. The new methods provide for isolation of WBC preparations with high yield, purity, and viability. The methods are designed for compatibility with automation protocols for rapid processing of multiple samples.
    Type: Application
    Filed: May 30, 2014
    Publication date: April 23, 2015
    Applicant: The General Hospital Corporation
    Inventors: Denise L. Faustman, Douglas E. Burger
  • Publication number: 20150104845
    Abstract: An acoustic manipulation process and acoustic manipulation device are disclosed. The acoustic manipulation process includes providing a device having a pathway positioned to receive a flow of a particle-containing fluid, transporting the particle-containing fluid into the pathway, and applying standing surface acoustic waves to the particle-containing fluid while the particle-containing fluid is flowing through the pathway. The applying of the standing surface acoustic waves to the particle-containing fluid includes nodes and anti-nodes of the standing surface acoustic waves extending through the particle-containing fluid. The acoustic manipulation device includes a pathway positioned to receive a flow of a particle-containing fluid, and a mechanism for generating and applying standing surface acoustic waves to the particle-containing fluid while the particle-containing fluid is flowing through the pathway.
    Type: Application
    Filed: October 14, 2014
    Publication date: April 16, 2015
    Inventors: Tony Jun HUANG, Yuchao CHEN
  • Patent number: 9005941
    Abstract: The present invention includes methods, devices and systems for isolating a nucleic acid from a fluid comprising cells. In various aspects, the methods, devices and systems may allow for a rapid procedure that requires a minimal amount of material and/or results in high purity nucleic acid isolated from complex fluids such as blood or environmental samples.
    Type: Grant
    Filed: September 4, 2014
    Date of Patent: April 14, 2015
    Assignee: Biological Dynamics, Inc.
    Inventors: Rajaram Krishnan, David Charlot, Eugene Tu, James McCanna, Lucas Kumosa, Paul D. Swanson, Robert Turner, Kai Yang, Irina Dobrovolskaya, David Liu
  • Patent number: 8975065
    Abstract: Methods and devices of performing reactions for which presence of light is desirable are provided. Biological or chemical materials such as algae are put in a chamber shaped as a meandering fluid channel. The algae can be combined with biomass such as human or animal waste and then subject to light, such as natural light or light coming from a LED, to produce fuel. Production of fuel can be optimized by controlling the height-to-width ratio of the channels.
    Type: Grant
    Filed: July 23, 2007
    Date of Patent: March 10, 2015
    Assignee: California Institute of Technology
    Inventors: George Maltezos, Axel Scherer
  • Publication number: 20150064764
    Abstract: A device for transporting, trapping and escaping a single biomaterial using a magnetic structure, and a method of transporting, trapping and escaping of the single biomaterial using the same are provided, and a method is provided for controlling movement and direction of the single biomaterial including soft magnetic micro structure and magnetic structure in a linear, square storage, apartment type, radial soft magnetic micro structure. Accordingly, the device for transporting, trapping and escaping a single biomaterial and the method for transporting, trapping and escaping single biomaterial using the same can control movement on the lap-on-a-chip with increased precision and ease, by using magnetic force, and thus can be advantageously used in the field of magneto-resistive sensor, or categorization of single cells or biomolecules.
    Type: Application
    Filed: August 29, 2013
    Publication date: March 5, 2015
    Applicant: The Industry & Academic Cooperation in Chungnam National University (IAC)
    Inventors: Cheolgi Kim, Byeonghwa Lim, Reddy Venu, Hu XingHao, KunWoo Kim, Benjamin B. Yellen
  • Patent number: 8969059
    Abstract: The present invention includes methods, devices and systems for isolating a nucleic acid from a fluid comprising cells. In various aspects, the methods, devices and systems may allow for a rapid procedure that requires a minimal amount of material and/or results in high purity nucleic acid isolated from complex fluids such as blood or environmental samples.
    Type: Grant
    Filed: February 28, 2014
    Date of Patent: March 3, 2015
    Assignee: Biological Dynamics, Inc.
    Inventors: Rajaram Krishnan, David Charlot, Eugene Tu, James McCanna, Lucas Kumosa, Paul Swanson, Robert Turner, Kai Yang, Irina Dobrovolskaya, David Liu
  • Patent number: 8969058
    Abstract: The present invention relates to systems for releasing genetic materials from a solid medium. The present invention also relates to methods for releasing genetic materials from a solid medium. The present invention further relates to methods for isolating genetic material from a biological sample.
    Type: Grant
    Filed: December 15, 2008
    Date of Patent: March 3, 2015
    Assignee: Canon U.S. Life Sciences, Inc.
    Inventor: Michele R. Stone
  • Patent number: 8962235
    Abstract: A method for sorting particles, in particular cells A and B. The method uses a single channel with only one input and only one output. A particle mix A and B in a fluid is introduced into the channel and particles within the channel are sorted.
    Type: Grant
    Filed: September 20, 2007
    Date of Patent: February 24, 2015
    Assignee: The University Court of the University of St. Andrews
    Inventors: Michael MacDonald, Kishan Dholakia, Igor Andreev
  • Publication number: 20150050710
    Abstract: The present invention relates to a living fatty material from which immunity is removed, and a method for manufacturing the same, and more specifically, to a living fat tissue from which immunity is removed and to a method for manufacturing the same, comprising the steps of collecting fat tissues; irradiating the fat tissues with 20 to 500 kGy of gamma (?) rays; and applying centrifugal separation of the fat tissues irradiated with gamma rays.
    Type: Application
    Filed: October 17, 2012
    Publication date: February 19, 2015
    Inventor: HEE YOUNG LEE
  • Publication number: 20150044180
    Abstract: The present invention relates to methods of isolating a substantially homogeneous population of pluripotent stem cells from adult neural crest tissue (e.g., periodontal ligament) as well as pharmaceutical compositions comprising such isolated pluripotent stem cells. Methods of inducing the isolated pluripotent stem cells into specific cell lineages, such as neurogenic and retinogenic lineages, are also described. The isolated pluripotent stem cells find use in various regenerative medicine applications and the treatment of degenerative diseases.
    Type: Application
    Filed: March 1, 2013
    Publication date: February 12, 2015
    Inventors: Herman S. Cheung, Daniel Pelaez, C-Y Charles Huang
  • Publication number: 20150044684
    Abstract: An object of the present invention is to prepare individual microorganisms or a microflora composition derived from land or seafloor soil in which contamination of land or seafloor soil-derived substances is reduced.
    Type: Application
    Filed: March 12, 2013
    Publication date: February 12, 2015
    Inventors: Sumihiro Koyama, Taishi Tsubouchi
  • Patent number: 8951765
    Abstract: Embodiments described herein relate to separating and/or concentrating target cells from a carrier fluid that may include other non-target cells. Embodiments include a cell separator with a flow surface having indentations formed thereon. The indentations are configured to capture target cells by physical and/or chemical interactions. The indentations may also include a layer of support molecules that assist in releasing captured cells for collection.
    Type: Grant
    Filed: November 24, 2009
    Date of Patent: February 10, 2015
    Assignee: Empire Technology Development LLC
    Inventor: Masahiro Ueda
  • Publication number: 20150037864
    Abstract: A process for separating sperm cells from a chemical compound by electrophoresis comprising subjecting the sperm cells to an electric potential between a cathode and an anode such that the sperm cells are separated from the chemical compound, and related methods including methods of using said sperm cells in intrauterine insemination.
    Type: Application
    Filed: March 15, 2013
    Publication date: February 5, 2015
    Inventor: Michael Howard James
  • Patent number: 8942458
    Abstract: A method for distinguishing and sorting cells characterized by comprising distinguishing and sorting a specific cell mass or a part of the cells in the cell mass with the use of transmitted light data reflecting the morphological characteristics of the cells such as size and shape optionally together with side-scattering light data reflecting the characteristics of the internal structure of the cells. The part of the cells in the specific cell mass as described above are at the G1 stage or at a part of the M stage in the cell cycle. A part of the cells at the G1 stage are referred to as the left bottom line in an analytical dispersion diagram of the cells wherein the abscissa indicates the transmitted light data, while a part of the cells at the M stage are referred to as the right bottom line in the analytical dispersion diagram of the cells wherein the abscissa indicates the transmitted light data.
    Type: Grant
    Filed: June 19, 2009
    Date of Patent: January 27, 2015
    Assignee: Furukawa Electric Co., Ltd.
    Inventors: Toru Takahashi, Ken Tsukii, Jie Xu
  • Patent number: 8932815
    Abstract: The present invention includes methods, devices and systems for isolating a nucleic acid from a fluid comprising cells. In various aspects, the methods, devices and systems may allow for a rapid procedure that requires a minimal amount of material and/or results in high purity nucleic acid isolated from complex fluids such as blood or environmental samples.
    Type: Grant
    Filed: June 20, 2014
    Date of Patent: January 13, 2015
    Assignee: Biological Dynamics, Inc.
    Inventors: Rajaram Krishnan, David J. Charlot, Eugene Tu, James McCanna, Lucas Kumosa, Paul D. Swanson, Robert Turner, Kai Yang, Irina Dobrovolskaya, David Liu, Juan Pablo Hinestrosa Salazar, Juscilene Menezes
  • Patent number: 8926815
    Abstract: Method for the selection or the processing of first particles sensitive to the application of an external stimulus including the step of producing, through the application of the external stimulus, the permeabilization of at least a selected first particle, consisting in the organization of the first particles through a first force field, to generate a second force field substantially placed in proximity of at least a selected first particle to be permeabilized.
    Type: Grant
    Filed: April 13, 2007
    Date of Patent: January 6, 2015
    Assignee: Silicon Biosystems S.p.A
    Inventors: Mélanie Abonnenc, Nicoló Manaresi, Gianni Medoro
  • Patent number: 8927243
    Abstract: The present invention concerns compositions and methods of extracting infectious pathogens from a volume of blood. In one embodiment, the method includes the steps of creating a fibrin aggregate confining the pathogens and introducing a fibrin lysis reagent to expose the pathogens for analysis. The present invention also concerns materials and methods for removing aurintricarboxylic acid (ATA) from a sample.
    Type: Grant
    Filed: December 21, 2012
    Date of Patent: January 6, 2015
    Assignee: University of South Florida
    Inventor: Matt Ewert
  • Patent number: 8921102
    Abstract: The invention features devices and methods for detecting, enriching, and analyzing circulating tumor cells and other particles. The invention further features methods of diagnosing a condition, e.g., cancer, in a subject by analyzing a cellular sample from the subject.
    Type: Grant
    Filed: December 29, 2005
    Date of Patent: December 30, 2014
    Assignee: GPB Scientific, LLC
    Inventors: Martin Fuchs, Ying-Xin Wang, Yi-Shuian Huang, Neil X. Krueger
  • Publication number: 20140377834
    Abstract: An acoustic standing wave is utilized to separate components from a multi-component fluid, such as animal cells from fluid-cell mixture, in a fluid flow scheme with an acoustophoresis device. For example, the flow scheme and device allows for trapping of falling cells as the cells coalesce, agglomerate, and the weight of the agglomerated mass overcomes the drag and ultrasonic standing wave forces in the device.
    Type: Application
    Filed: June 24, 2014
    Publication date: December 25, 2014
    Inventors: Walter M. Presz, Jr., Bart Lipkiens, Jason Dionne, Thomas J. Kennedy, III
  • Publication number: 20140349330
    Abstract: The invention relates to a process which enables optimal aggregation of cells, typically of stem cells, promoting the organisation thereof and advantageously the differentiation thereof, in particular in the context of the formation of a tissue substitute. This process comprises exposing pretreated cells to a magnetic field and makes it possible to obtain large cell aggregates, even prepared in the absence of support matrix and/or of growth factor. The invention also relates to the cell aggregates that can be obtained using such a process and also to the uses thereof as tissue initiators with a view to obtaining a tissue structure of interest in vitro, ex vivo or in vivo. Moreover, it relates to the resulting tissue structures and to the uses thereof in research or in therapy as tissue substitutes. The present application also provides a method which advantageously makes it possible to monitor the development of the tissue of interest in vivo.
    Type: Application
    Filed: September 3, 2012
    Publication date: November 27, 2014
    Applicant: CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE
    Inventors: Delphine Fayol, Nathalie Luciani, Catherine Le Visage, Florence Gazeau, Claire Wilhelm-Hannetel
  • Patent number: 8889388
    Abstract: The invention provides a settling device comprising an acoustic wave generator and an inclined settling chamber. The angle ? between the acoustic wave direction and the inclined settling chamber is greater than 0 and less than 90°. The invention also provides a concentration method and a separation method using the device. The invention can be used to concentrate or separate particles such as inorganic particles, organic particles, and biological particles, for example, mammalian cells, bacteria, yeast, algae, and plant cells. The invention exhibits technical merits such as higher efficiency, cost-effectiveness, and large-scale production.
    Type: Grant
    Filed: April 21, 2011
    Date of Patent: November 18, 2014
    Inventors: Zhaowei Wang, Donald Feke, Joanne Belovich
  • Patent number: 8877470
    Abstract: The present invention includes methods, devices and systems for isolating a nucleic acid from a fluid comprising cells. In various aspects, the methods, devices and systems may allow for a rapid procedure that requires a minimal amount of material and/or results in high purity nucleic acid isolated from complex fluids such as blood or environmental samples.
    Type: Grant
    Filed: March 7, 2014
    Date of Patent: November 4, 2014
    Assignee: Biological Dynamics, Inc.
    Inventors: Rajaram Krishnan, David Charlot, Eugene Tu, James McCanna, Lucas Kumosa, Paul Swanson, Robert Turner, Kai Yang, Irina Dobrovolskaya, David Liu
  • Patent number: 8871481
    Abstract: The present invention includes methods, devices and systems for isolating a nucleic acid from a fluid comprising cells. In various aspects, the methods, devices and systems may allow for a rapid procedure that requires a minimal amount of material and/or results in high purity nucleic acid isolated from complex fluids such as blood or environmental samples.
    Type: Grant
    Filed: May 6, 2014
    Date of Patent: October 28, 2014
    Assignee: Biological Dynamics, Inc.
    Inventors: Rajaram Krishnan, David Charlot, Eugene Tu, James McCanna, Lucas Kumosa, Paul Swanson, Robert Turner, Kai Yang, Irina Dobrovolskaya, David Liu
  • Publication number: 20140302580
    Abstract: The invention provides a method of dispersing or circulating magnetically responsive beads within a droplet in a droplet actuator. The invention, in one embodiment, makes use of a droplet actuator with a plurality of droplet operations electrodes configured to transport the droplet, and a magnetic field present at a portion of the plurality of droplet operations electrodes. A bead-containing droplet is provided on the droplet actuator in the presence of the uniform magnetic field. Beads are circulated in the droplet during incubation by conducting droplet operations on the droplet within a uniform region of the magnate field. Other embodiments are also provided.
    Type: Application
    Filed: June 18, 2014
    Publication date: October 9, 2014
    Applicant: Advanced Liquid Logic, Inc.
    Inventors: Ramakrishna Sista, Vamsee K. Pamula, Vijay Srinivasan, Michael G. Pollack, Allen Eckhardt
  • Patent number: 8841104
    Abstract: The invention generally relates to methods of using compositions that include sets of magnetic particles, members of each set being conjugated to an antibody specific for a pathogen, and magnets to isolate a pathogen from a body fluid sample.
    Type: Grant
    Filed: April 21, 2011
    Date of Patent: September 23, 2014
    Assignee: NanoMR, Inc.
    Inventors: Sergey A. Dryga, Victor C. Esch, Lisa-Jo Ann Clarizia, Eddie W. Adams, Thearith H. Ung, Ravil A. Sitdikov
  • Publication number: 20140255356
    Abstract: Methods of isolating stromal vascular fractions for use in human subjects from a tissue comprising a blood vessel using mechanical, optionally ultrasonic cavitation, enzymatic, and/or chemical methods are provided. These methods yield an increased number of the cells which constitute the stromal vascular fractions (about 10-fold greater) than methods which use collagenase to isolate these cells. The stromal vascular fraction may be isolated from any tissue that comprises blood vessels, for example, muscle and adipose tissue. Additionally, the tissue may be isolated obtained from nonliving sources and animals (e.g., postmortem).
    Type: Application
    Filed: March 6, 2014
    Publication date: September 11, 2014
    Inventor: Steven VICTOR
  • Publication number: 20140248679
    Abstract: The present invention discloses apparatus and methods to enhance magnetic field gradient for magnetic cell separation. One preferred embodiment in accordance with the invention comprises an electromagnet with an open gap formed between two pole tips and two driving coils with independently controlled electrical current magnitude and direction. In the specific case when two magnetic pole tips emanate magnetic fields with different magnitude and in opposite direction, the field gradient, and therefore the magnetic force exerted on MNPs, is advantageously enhanced. Spatial uniformity of the magnetic field gradient is also enhanced. Preferred embodiments are capable of magnetic cell separation for both positive and negative cell selection, as well as in-vitro capture and separation in miniaturized form of embodiments.
    Type: Application
    Filed: February 28, 2014
    Publication date: September 4, 2014
    Inventors: Jing Zhang, Glenn Yaguang Deng
  • Publication number: 20140248621
    Abstract: Microfluidic devices and methods that use cells such as cancer cells, stem cells, blood cells for preprocessing, sorting for various biodiagnostics or therapeutical applications are described. Microfluidics electrical sensing such as measurement of field potential or current and phenomena such as immiscible fluidics, inertial fluidics are used as the basis for cell and molecular processing (e.g., characterizing, sorting, isolation, processing, amplification) of different particles, chemical compositions or biospecies (e.g., different cells, cells containing different substances, different particles, different biochemical compositions, proteins, enzymes etc.). Specifically this invention discloses a few sorting schemes for stem cells, whole blood and circulating tumor cells and also extracting serum from whole blood. Further medical diagnostics technology utilizing high throughput single cell PCR is described using immiscible fluidics couple with single or multi cells trapping technology.
    Type: Application
    Filed: January 10, 2013
    Publication date: September 4, 2014
    Inventor: John COLLINS
  • Patent number: 8822211
    Abstract: The invention concerns a device and a method for concentrating pathogenic germs potentially present in blood products or derivatives and for detecting said germs comprising the following steps: (a) subjecting a sample of said blood product to a blood cell aggregating treatment, (b) eliminating the aggregates formed at step (a) by passing the treated sample over a first filter allowing through the contaminating germs but not the cell aggregates, (c) selectively lyzing the residual cells of the filtrate obtained at step (b), (d) recuperating the contaminating germs by passing the lysate of step (c) over a second filter to detect the contaminating germs possibly trapped.
    Type: Grant
    Filed: June 11, 2013
    Date of Patent: September 2, 2014
    Assignee: Becton Dickinson Infusion Therapy Systems Inc.
    Inventors: Jean-Pierre Hermet, Isabelle Besson-Faure, Sebastien Ribault, Yann Godfrin, Anne Monnot des Angles
  • Publication number: 20140242663
    Abstract: Described herein are methods and systems for harvesting, collecting, separating and/or dewatering algae using iron based salts combined with a magnetic field gradient to separate algae from an aqueous solution.
    Type: Application
    Filed: February 25, 2014
    Publication date: August 28, 2014
    Applicant: Los Alamos National Security, LLC
    Inventors: Pulak Nath, Scott N. Twary
  • Patent number: 8815555
    Abstract: The present invention includes methods, devices and systems for isolating a nucleic acid from a fluid comprising cells. In various aspects, the methods, devices and systems may allow for a rapid procedure that requires a minimal amount of material and/or results in high purity nucleic acid isolated from complex fluids such as blood or environmental samples.
    Type: Grant
    Filed: March 7, 2014
    Date of Patent: August 26, 2014
    Assignee: Biological Dynamics, Inc.
    Inventors: Rajaram Krishnan, David Charlot, Eugene Tu, James McCanna, Lucas Kumosa, Paul Swanson, Robert Turner, Kai Yang, Irina Dobrovolskaya, David Liu
  • Patent number: 8815554
    Abstract: The present invention includes methods, devices and systems for isolating a nucleic acid from a fluid comprising cells. In various aspects, the methods, devices and systems may allow for a rapid procedure that requires a minimal amount of material and/or results in high purity nucleic acid isolated from complex fluids such as blood or environmental samples.
    Type: Grant
    Filed: February 28, 2014
    Date of Patent: August 26, 2014
    Assignee: Biological Dynamics, Inc.
    Inventors: Rajaram Krishnan, David Charlot, Eugene Tu, James McCanna, Lucas Kumosa, Paul Swanson, Robert Turner, Kai Yang, Irina Dobrovolskaya, David Liu
  • Publication number: 20140234864
    Abstract: A method for magnetic cellular manipulation may include contacting a composition with a biological sample to form a mixture. The composition may include a plurality of particles. Each particle in the plurality of particles may include a magnetic substrate. The magnetic substrate may be characterized by a magnetic susceptibility greater than zero. The composition may also include a chargeable silicon-containing compound. The chargeable silicon-containing compound may coat at least a portion of the magnetic substrate. The biological sample may include cells and/or cellular structures. The method may also include applying a magnetic field to the mixture to manipulate the composition.
    Type: Application
    Filed: August 23, 2013
    Publication date: August 21, 2014
    Inventor: Kristie Marie Krug
  • Patent number: 8795497
    Abstract: Disclosed herein is a cell sorter including a measuring electrode, working electrode, detection electrode, and output section. The measuring electrode forms a measuring electric field in a flow path to measure a complex dielectric constant of each cells flowing through the flow path. The working electrode forms, in the flow path, a working electric field to sort the cells by imparting a dielectrophoretic force to the cells and using the flow path. The detection electrode detects the presence of the cell in the fluid flowing through the flow path. The output section acquires a sorting signal based on information about the measured complex dielectric constant and a detection signal indicating the detection of the cell by the detection electrode. The output section outputs a working signal adapted to form the working electric field to the working electrode when the detection signal is acquired if the sorting signal is acquired.
    Type: Grant
    Filed: October 13, 2011
    Date of Patent: August 5, 2014
    Assignee: Sony Corporation
    Inventors: Kazumasa Sato, Yoichi Katsumoto
  • Patent number: 8795999
    Abstract: This invention relates to adsorbents and methods for highly selective removal of anti-von Willebrand Factor-cleaving protease antibodies (“anti-vWF-cp-abs”) from human plasma using human von Willebrand Factor-cleaving protease (“hvWF-cp”) or fragments thereof as affinity ligands. The adsorbents can be used for treating disorders associated with the occurrence of anti-vWF-cp-abs in patients, such as thromboembolic diseases.
    Type: Grant
    Filed: February 18, 2009
    Date of Patent: August 5, 2014
    Assignees: Baxter International Inc., Baxter Healthcare SA
    Inventors: Friedrich Scheiflinger, Barbara Plaimauer, Gerhard Antoine
  • Patent number: 8795498
    Abstract: The invention comprises two key components: dielectrophoresis (DEP) and reversible binding surfaces. DEP has become an important tool for trapping dielectric particles. Moreover, DEP can manipulate cell movement as dictated by the intrinsic dielectric constant of the cell without modification. DEP therefore provides a mechanism by which to force targets in a flow channel to a reversible binding surface. By building selectivity into the binding surface, the capacity to choose which targets can be held after the dielectric field is turned off, providing a separation strategy that does not suffer from fouling issues, as large foulants can freely pass over the surface through the flow channel.
    Type: Grant
    Filed: October 17, 2013
    Date of Patent: August 5, 2014
    Assignee: University of South Florida
    Inventors: Ryan Toomey, Peter George Stroot
  • Patent number: 8785141
    Abstract: The invention aims at providing an adsorbent for bacterial toxins, a method for removal of such toxins by adsorption, and an adsorber packed with said adsorbent. Provided are an adsorbent for bacterial toxins, which comprises a water-insoluble porous material having a mode of pore radius of 20 angstroms to 1,000 angstroms, a method for removal of bacterial toxins using said adsorbent, and an adsorber packed with said adsorbent.
    Type: Grant
    Filed: August 24, 2001
    Date of Patent: July 22, 2014
    Assignee: Kaneka Corporation
    Inventors: Tamiji Fujimoto, Fumiyasu Hirai, Shigeo Furuyoshi
  • Publication number: 20140199747
    Abstract: A magnetic platform is provided and includes a patterned array of discrete magnetic elements positioned on a substrate, a plurality of first electromagnets for creating a first magnetic field substantially in the plane of the substrate, an electromagnetic coil for creating a second magnetic field substantially perpendicular to the plane of the substrate, and a control device for controlling the application of the magnetic fields. Processes for manipulating, transporting, separating and sorting micro- or nano-scale particles and biomolecules are also described.
    Type: Application
    Filed: March 14, 2014
    Publication date: July 17, 2014
    Inventors: Ratnasingham Sooryakumar, Thomas Charles Henighan, Gregory B. Vieira, Jeffrey J. Chalmers
  • Patent number: 8778154
    Abstract: To provide a system by which evaluation of circumstances of contamination by microparticles having nucleic acid can be performed rapidly and accurately. The theme is achieved by a system for measuring microparticles that includes: (1) a microparticle adhesion step of adhering the microparticles having nucleic acid to a microparticle adhesion member; (2) a membrane breakage step of breaking membranes of the adhered microparticles by electrical discharge; (3) an electrophoresis step of electrophoresing the microparticles in a thickness direction of a gel to make the nucleic acid in the microparticles migrate from a negative electrode side toward a positive electrode side and adhere the nucleic acid on a surface of a nucleic acid detection member; and (4) a nucleic acid measurement step of fluorescently staining the surface of the nucleic acid detection member to measure a concentration of the nucleic acid.
    Type: Grant
    Filed: December 17, 2009
    Date of Patent: July 15, 2014
    Assignee: Toyohashi University of Technology
    Inventors: Akira Mizuno, Kazunori Takashima, Hachiro Yasuda, Masudur Rahman
  • Patent number: 8772004
    Abstract: A method and device for aggregating algae in an aqueous solution is disclosed. The method can include providing an algae feed comprising a liquid and algae dispersed therein. The algae feed can be aggregated by applying a nanosecond pulsed electric field to the algae feed. The nanosecond pulsed electric field can include a plurality of electric pulses having a pulse duration ranging from 1 to 1,000 nanoseconds. The method can also include separating an aggregated algae stream from the algae feed and feeding the aggregated algae stream to a lipid extraction operation.
    Type: Grant
    Filed: June 25, 2010
    Date of Patent: July 8, 2014
    Assignee: Old Dominion University Research Foundation
    Inventors: Gary C. Schafran, Juergen F. Kolb, Aron Stubbins, Karl H. Schoenbach