Treatment Of Micro-organisms Or Enzymes With Electrical Or Wave Energy (e.g., Magnetism, Sonic Waves, Etc.) Patents (Class 435/173.1)
  • Patent number: 10843148
    Abstract: A new simple transfection method using an integrated electrowetting nano-injector (“INENI”) with controlled dosage delivery and high transfection efficiency is disclosed. The volume of delivery can be controlled via voltage application to an inner and outer electrode integrated into a nano-pipette. With higher voltages, more liquid enters the INENI and with lower voltages liquid is expelled. This method can be used to deliver plasmid DNA directly into the nuclei of cells. The INENI requires only the use of a single probe since both electrodes are integrated into the same nano-pipette. Hence, more space is available, and ergo the INENI offers a simplistic means for direct injection of metered amounts of exogenous material into the confines of a cell cytoplasm and/or nucleus while retaining full cell viability.
    Type: Grant
    Filed: June 19, 2018
    Date of Patent: November 24, 2020
    Assignee: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
    Inventors: Hemantha K. Wickramasinghe, Elaheh Shekaramiz
  • Patent number: 10808262
    Abstract: The invention provides non-naturally occurring microbial organisms containing enzymatic pathways and/or metabolic modifications for enhancing carbon flux through acetyl-CoA. In some embodiments, the microbial organisms having such pathways also include pathways for generating reducing equivalents, formaldehyde fixation and/or formate assimilation. The enhanced carbon flux through acetyl-CoA, in combination with pathways for generating reducing equivalents, formaldehyde fixation and/or formate assimilation can, in some embodiments, be used for production of a bioderived compound. Accordingly, in some embodiments, the microbial organisms of the invention can include a pathway capable of producing a bioderived compound of the invention.
    Type: Grant
    Filed: November 25, 2014
    Date of Patent: October 20, 2020
    Assignee: Genomatica, Inc.
    Inventors: Robin E. Osterhout, Anthony P. Burgard, Priti Pharkya, Stefan Andrae
  • Patent number: 10450543
    Abstract: Provided herein is a pipette tip for electroporation including an outer surface, a void located within the outer surface, and a conductor located at least partially on or within the outer surface, in electrical communication with at least a portion of the outer surface and the void. Further provided herein is a pipette tip for electroporation including a body, a connector, and an elongated part. The connector is located at the distal end of the body and further includes at least one connecting post, a connecting part in mechanical communication with the connector, and a conductor located at least partially on or within the connector, wherein the conductor surrounds at least a portion of the connecting post. The elongated part also has a void and is located at the distal end of the connector. The void of the body, connector, and elongated part are in fluid communication.
    Type: Grant
    Filed: November 21, 2016
    Date of Patent: October 22, 2019
    Assignee: LIFE TECHNOLOGIES CORPORATION
    Inventors: Jun-Keun Chang, Keun-Chang Cho, Chan-Il Chung, Neon-Cheol Jung, Seung Gin Huh
  • Patent number: 10434086
    Abstract: Provided herein are methods and compositions that relate to, inter alia, CBL0137, a carbazole compound 1,1?-[9-[2-[(1-methylethyl)amino]ethyl]-9H-carbazole-3,6-diyl]bis-ethanone, useful for the treatment of various cancers, optionally in combination with various agents.
    Type: Grant
    Filed: April 6, 2015
    Date of Patent: October 8, 2019
    Assignee: Incuron, Inc.
    Inventor: Catherine Burkhart
  • Patent number: 10400208
    Abstract: A method of discriminating a living cell from a dead cell, and a device for implementing the method, the method including: using a lens-free imaging device to acquire a diffraction figure corresponding to a cell; and determining a light intensity on a central area of an elementary diffraction figure associated with the cell. It can thus be determined if the studied cell is a living cell or a dead cell.
    Type: Grant
    Filed: July 21, 2014
    Date of Patent: September 3, 2019
    Assignee: COMMISSARIAT A L'ENERGIE ATOMIQUE ET AUX ENERGIES ALTERNATIVES
    Inventor: Cedric Allier
  • Patent number: 10365264
    Abstract: A measuring apparatus relates to a contractile ability of a spontaneously pulsating myocardial tissue. The measuring apparatus includes a measurement table and a pressure sensor. The measurement table includes a storage section having an opening and configured to hold liquid inside the storage section, and an attaching portion on which the myocardial tissue is attached around the opening. The pressure sensor is disposed inside the storage section.
    Type: Grant
    Filed: August 31, 2017
    Date of Patent: July 30, 2019
    Assignees: NIHON KOHDEN CORPORATION, TOKYO WOMEN'S MEDICAL UNIVERSITY
    Inventors: Hirotsugu Kubo, Tatsuya Shimizu, Tetsutaro Kikuchi
  • Patent number: 10351811
    Abstract: A cell culture container characterized by being equipped with a container main body and a flat plate attached onto one surface of the container main body, wherein the container main body is equipped with an inflow port through which a liquid can flow into the container main body, a passage through which the liquid flowing into the container main body from the inflow port can pass, and an outflow port through which the liquid passing through the passage can flow out from the container main body, and wherein, on the bottom surface of the passage, multiple cell-seeding areas in which cells passing through the passage can be seeded are arranged side by side along the passage.
    Type: Grant
    Filed: August 18, 2016
    Date of Patent: July 16, 2019
    Assignee: SINFONIA TECHNOLOGY CO., LTD.
    Inventors: Tomoaki Kurakazu, Yasuhiro Oshima, Yoshio Kimura, Kenichi Kagawa, Shinichi Gomi, Shigenori Ozaki
  • Patent number: 10258987
    Abstract: The present invention generally relates to the manipulation of fluids using acoustic waves such as surface acoustic waves. In some aspects, one fluid may be introduced into another fluid via application of suitable acoustic waves. For example, a fluid may be added or injected into another fluid by applying acoustic waves where, in the absence of the acoustic waves, the fluid cannot be added or injected, e.g., due to the interface or surface tension between the fluids. Thus, for example, a fluid may be injected into a droplet of another fluid. Other embodiments of the invention are generally directed to systems and methods for making or using such systems, kits involving such systems, or the like.
    Type: Grant
    Filed: June 25, 2015
    Date of Patent: April 16, 2019
    Assignees: President and Fellows of Harvard College, The University Court of the University of Glasgow
    Inventors: David A. Weitz, Thomas Franke
  • Patent number: 10219751
    Abstract: Methods, systems and related apparatus are provided to enable an electronic device to operate an external sensor comprising one or more emitters for emitting electromagnetic radiation of two different wavelengths and a detector for generating a response signal based on received electromagnetic radiation of the two different wavelengths connectable to an audio interface by applying a harmonic driving signal to a first contact and a second contact of the audio interface for driving the emitters of the external sensor, receiving the response signal at a third contact of the audio interface, demodulating and demultiplexing the response signal into a first wavelength response signal and a second wavelength response signal, analyzing the first and second wavelength response signals to determine one or more vital signs, and outputting the determined one or more vital signs.
    Type: Grant
    Filed: January 21, 2015
    Date of Patent: March 5, 2019
    Assignee: Lionsgate Technologies, Inc.
    Inventors: Christian Leth Petersen, John Mark Ansermino, Guy Dumont
  • Patent number: 10117701
    Abstract: A new method for the ablation of undesirable tissue such as cells of a cancerous or non-cancerous tumor is disclosed. It involves the placement of electrodes into or near the vicinity of the undesirable tissue through the application of electrical pulses causing irreversible electroporation of the cells throughout the entire area of the undesirable tissue. The electric pulses irreversibly permeate the cell membranes, thereby invoking cell death. The irreversibly permeabilized cells are left in situ and are removed by the body immune system. The amount of tissue ablation achievable through the use of irreversible electroporation without inducing thermal damage is considerable.
    Type: Grant
    Filed: March 5, 2015
    Date of Patent: November 6, 2018
    Assignee: The Regents of the University of California
    Inventors: Rafael Davalos, Boris Rubinsky
  • Patent number: 9938518
    Abstract: Devices are provided for inhibiting the electrical activity of an excitable cell through the application of a pulse of light which includes a substrate and a photoreactive film both of non-conducting material and laid directly on one another, in which the photoreactive film includes a layer of semiconductor polymer material and has an interface surface which can be placed in contact with an excitable cell and an electrolyte solution. After absorbing light, when placed in contact with the excitable cell and the electrolyte solution, the photoreactive film produces a potential difference across this interface surface which is capable of giving rise to hyperpolarization of the membrane of the excitable cell. Methods of inhibiting the electrical activity of excitable cells using such devices are also provided.
    Type: Grant
    Filed: August 1, 2014
    Date of Patent: April 10, 2018
    Assignees: FONDAZIONE ISTITUTO ITALIANO DI TECNOLOGIA, ISTITUTO DON CALABRIA—OSPEDALE CLASSIFICATO SACRO CUORE
    Inventors: Diego Ghezzi, Fabio Benfenati, Guglielmo Lanzani, Maria Rosa Antognazza, Giuliano Freddi, Ilaria Donelli, Maurizio Mete, Grazia Pertile
  • Patent number: 9868938
    Abstract: Methods of generating cardiomyocytes from induced pluripotent stem cells (IPSCs) are provided. More specifically, the present disclosure relates to methods of generating cardiomyocytes from iPSCs using electrical stimulation. In some aspects, uses of such cells for therapeutics and in methods of treatment are provided.
    Type: Grant
    Filed: January 14, 2016
    Date of Patent: January 16, 2018
    Assignee: University of Cincinnati
    Inventors: Yi-Gang Wang, Ha Won Kim, Ruilian Ma
  • Patent number: 9751057
    Abstract: A method of using a fluidics apparatus for lysing a cell. In the method, the cell is placed in a fluid sample contacting a substrate surface. The method further includes providing surface acoustic waves (SAWs) at the substrate surface, causing cell lyses.
    Type: Grant
    Filed: May 27, 2016
    Date of Patent: September 5, 2017
    Assignee: The University Court of the University of Glasgow
    Inventors: Rab Wilson, Jonathan M. Cooper, Julien Reboud
  • Patent number: 9708597
    Abstract: In accordance with the present disclosure, exposure of a sample to one or more electric pulses via capacitive coupling is described. In certain embodiments, the sample may be a biological sample to be treated or modified using the pulsed electric fields. In certain embodiments, the electric pulses may be delivered to a load using capacitive coupling. In other embodiments, the electric pulses may be bipolar pulses.
    Type: Grant
    Filed: January 17, 2014
    Date of Patent: July 18, 2017
    Assignee: GENERAL ELECTRIC COMPANY
    Inventors: Antonio Caiafa, Vasile Bogdan Neculaes, Allen Lawrence Garner, Andrew Soliz Torres
  • Patent number: 9688955
    Abstract: A material comprising positively and negatively charged nanoparticles, wherein one of said nanoparticles contained a magnetically responsive element, are combined with a support molecule, which is a long natural or synthetic molecule or polymer to make a magnetic nanoparticle assembly. When the magnetic nanoparticle assembly is combined with cells, it will magnetize those cells. The magnetized cells can then be washed to remove the magnetic nanoparticle assembly and the magnetized cells manipulated in a magnetic field.
    Type: Grant
    Filed: November 7, 2014
    Date of Patent: June 27, 2017
    Assignee: Nano3D Biosciences, Inc.
    Inventor: Glauco R. Souza
  • Patent number: 9663762
    Abstract: Methods for recovering target cells using degradable three dimensional matrices are described.
    Type: Grant
    Filed: May 16, 2013
    Date of Patent: May 30, 2017
    Assignee: Ingeneron, Inc.
    Inventors: Michael Coleman, Jody Vykoukal
  • Patent number: 9617344
    Abstract: A fusion protein including a targeting moiety, a cleavage site, and a cell membrane penetrating domain, a conjugate including the fusion protein and a bioactive molecule, and methods employing the fusion protein and the conjugate are provided.
    Type: Grant
    Filed: July 8, 2015
    Date of Patent: April 11, 2017
    Assignee: SAMSUNG ELECTRONICS CO., LTD.
    Inventors: Jae Il Lee, Soshin Ahn, Jung Min Kim, Jungmin Lee, Jung-Hoon Lee
  • Patent number: 9550981
    Abstract: Fusion proteins comprising a tafazzin peptide and a cellular permeability peptide are disclosed. The tafazzin peptide may be coupled to the permeability peptide through a polypeptide linker. Methods of making and using the fusion proteins are also disclosed. For example, the disclosed fusion proteins may be used to treat a patient having a disorder associated with a tafazzin deficiency or a remodeled cardiolipin deficiency (e.g., Barth syndrome). Additionally, the disclosed fusion proteins may be used in prophylaxis against developing a disorder associated with a tafazzin deficiency or a remodeled cardiolipin deficiency in a patient at risk of developing such a disorder.
    Type: Grant
    Filed: January 22, 2015
    Date of Patent: January 24, 2017
    Assignee: University of Washington
    Inventors: Michael T. Chin, Wei-Ming Chien, Ana Dinca
  • Patent number: 9523070
    Abstract: A photobioreactor includes an input light delivery component and a plurality of photobioreactor media disposed in a stacked relationship, each of which is coupled to the input light delivery component, wherein each photobioreactor medium is adapted to be inoculated with a photosynthetic, biofuel-producing culture, further wherein each photobioreactor medium includes at least one hollow fiber membrane (HFM) disposed thereon in a region that is adapted to be inoculated with the photosynthetic, biofuel-producing culture, that can transport at least one of a gas and a photobioreactor product producible by the photosynthetic, biofuel-producing culture, wherein each photobioreactor medium is characterized by an input light-scattering mechanism.
    Type: Grant
    Filed: March 7, 2014
    Date of Patent: December 20, 2016
    Assignee: CORNELL UNIVERSITY
    Inventors: David Erickson, Largus Angenent, Devin Doud, Erica Jung, Michael Kalontarov
  • Patent number: 9458515
    Abstract: An RNA of the present invention is an RNA containing a 5? cap structure and a coding region having a 5? initiation codon and a 3? stop codon on both ends of the coding region, the RNA having a nucleoside compound introduced at a site selected from among the 5? cap structure and 10 bases from a 5? end of the RNA, wherein the nucleoside compound is such that a group is attached to (i) a carbon atom at position 8 of a purine nucleus or (ii) a carbon atom at position 5 or 6 of a pyrimidine nucleus, the group being represented by formula (I): A-X?X-#??(I) where A represents an aryl group or a heteroaryl group, # represents a site where the group represented by the formula (I) is attached to the carbon atom at the position 8 of the purine nucleus or the carbon atom at the position 5 or 6 of the pyrimidine nucleus, and two Xs, which are identical to or different from each other, each represents a nitrogen atom or CH whose H may be substituted by alkyl.
    Type: Grant
    Filed: February 18, 2011
    Date of Patent: October 4, 2016
    Assignee: RIKEN
    Inventors: Shinji Ogasawara, Mizuo Maeda
  • Patent number: 9402814
    Abstract: A method is described including passing a solution having a biodegradable polymer, a solvent and a treatment agent through an electrocharged nozzle to form particles encapsulating the treatment agent. The particles emitted from the electrocharged nozzle may be exposed to a charge opposite that of the nozzle. The particles may be deposited in a collection assembly comprising a liquid phase. A further method including combining a biodegradable polymer, a solvent and a treatment agent to form a solution, electrodepositing the solution in a particle form wherein the particles encapsulate the treatment agent in a collection assembly comprising a liquid phase and mixing the particles with a bioerodible material capable of forming a gel is described. An apparatus having an electrocharged nozzle, a grounded electrode having an opposite charge to that of the nozzle and a collection assembly comprising a liquid phase is further disclosed.
    Type: Grant
    Filed: June 9, 2014
    Date of Patent: August 2, 2016
    Assignee: Abbott Cardiovascular Systems Inc.
    Inventors: Shubhayu Basu, Eugene T. Michal
  • Patent number: 9228261
    Abstract: A method and apparatus are provided for constructing tissue from cells or other objects by application of temporally and spatially controlled electric fields. Electric field applicators expose a substrate (32) to the electric field controlled to affect the processing medium (28) to achieve a processing effect on the construction of tissue on the substrate (32). Electrical bias is selected to interact with dipole properties of the medium (28) to control the movement of suspended dielectrophoretic cells or other particles in the medium (28) or at the substrate (32). The motion of suspended particles may be affected to cause suspended particles of different properties to follow different paths in the processing medium (28), which may be used to cause the suspended particles to be sorted. The processing medium (28) and electrical bias may be selected to affect the structure, or orientation, of one or more layers on the substrate (32).
    Type: Grant
    Filed: July 31, 2012
    Date of Patent: January 5, 2016
    Assignee: Tokyo Electron Limited
    Inventor: Jozef Brcka
  • Patent number: 9217180
    Abstract: Disclosed are an oligonucleotide primer with an omega structure for detecting short-chain RNAs and the use thereof. The primer from the 5? end to the 3? end sequentially is: a PCR primer target region of 20-30 bases, a variable coding region of 0-50 bases, an omega stem-loop, a probe spacer of at least one base and a probe region of 4-11 bases. The length of the stem of the omega stem-loop is 4-12 paired bases, and the length of the loop of the omega stem-loop is 3-20 unpaired bases.
    Type: Grant
    Filed: January 16, 2013
    Date of Patent: December 22, 2015
    Assignee: Chengdu Nuoen Biological Technology Co., Ltd.
    Inventors: Songbo Zhang, Guobiao Jiang, Fang Tang, Feifei Zhang
  • Patent number: 9206416
    Abstract: The present invention includes methods, devices and systems for isolating a nucleic acid from a fluid comprising cells. In various aspects, the methods, devices and systems may allow for a rapid procedure that requires a minimal amount of material and/or results in high purity nucleic acid isolated from complex fluids such as blood or environmental samples.
    Type: Grant
    Filed: October 30, 2013
    Date of Patent: December 8, 2015
    Assignee: BIOLOGICAL DYNAMICS, INC.
    Inventors: Rajaram Krishnan, David Charlot, Eugene Tu, James McCanna, Lucas Kumosa, Paul Swanson, Robert Turner, Kai Yang, Irina Dobrovolskaya, David Liu
  • Patent number: 9040272
    Abstract: The present invention provides a method and system for using eye-safe infrared energy from a Class I laser to manipulate cells in culture. The laser energy produces one or more phase boundary propulsion events, which generate hydrodynamic forces sufficient to manipulate cells at the focal point.
    Type: Grant
    Filed: June 13, 2011
    Date of Patent: May 26, 2015
    Assignee: Hamilton Thorne, Inc.
    Inventors: Diarmaid H. Douglas-Hamilton, Thomas G. Kenny
  • Patent number: 9034578
    Abstract: The present invention includes methods, devices and systems for isolating a nucleic acid from a fluid comprising cells. In various aspects, the methods, devices and systems may allow for a rapid procedure that requires a minimal amount of material and/or results in high purity nucleic acid isolated from complex fluids such as blood or environmental samples.
    Type: Grant
    Filed: October 7, 2014
    Date of Patent: May 19, 2015
    Assignee: BIOLOGICAL DYNAMICS, INC.
    Inventors: Rajaram Krishnan, David Charlot, Eugene Tu, James McCanna, Lucas Kumosa, Paul Swanson, Robert Turner, Kai Yang, Irina Dobrovolskaya, David Liu
  • Patent number: 9034579
    Abstract: The present invention includes methods, devices and systems for isolating a nucleic acid from a fluid comprising cells. In various aspects, the methods, devices and systems may allow for a rapid procedure that requires a minimal amount of material and/or results in high purity nucleic acid isolated from complex fluids such as blood or environmental samples.
    Type: Grant
    Filed: October 10, 2014
    Date of Patent: May 19, 2015
    Assignee: BIOLOGICAL DYNAMICS, INC
    Inventors: Rajaram Krishnan, David J. Charlot, Eugene Tu, James McCanna, Lucas Kumosa, Paul D. Swanson, Robert Turner, Kai Yang, Irina Dobrovolskaya, David Liu, Juan Pablo Hinestrosa Salazar, Juscilene Menezes
  • Patent number: 9034622
    Abstract: The invention relates to a preparation for the photodynamic control of micro-organisms, said preparation being in a liquid or pasty form and containing a photosensitizer which comprises a dyestuff and produces singlet oxygen when irradiated by means of light. The micro-organisms can be marked by means of the dyestuff. The aim of the invention is to improve said preparation in order to enable an improved photodynamic control. To this end, the preparation contains an active ingredient for amplifying or weakening the oxidative action of the singulet oxygen by chemical manipulation of the dyestuff or the nanoenvironment thereof. In a particular form of embodiment, a rinsing solution is used before the irradiation.
    Type: Grant
    Filed: July 20, 2010
    Date of Patent: May 19, 2015
    Assignee: bredent medical GmbH & Co. KG
    Inventors: Freimut Vizethum, Reinhold Schuetze
  • Patent number: 9034254
    Abstract: Disclosed is an antibacterial composition comprising titanium oxide particles immobilized with an antibody having affinity and cognitive power to a microorganism of interest, and a method for sterilizing the microorganism by using the same. In particular, the present invention relates to a method for preparing functional titanium oxide particles capable of recognizing a microorganism or a virus of interest, and a method for selectively and efficiently sterilizing the same by using the functional titanium oxide particles, and not for randomly sterilizing microorganisms or viruses by using conventional titanium oxide particles having no recognition power to a microorganism or a virus of interest.
    Type: Grant
    Filed: November 8, 2012
    Date of Patent: May 19, 2015
    Assignee: KOREA INSTITUTE OF SCIENCE AND TECHNOLOGY
    Inventors: Byoung Chan Kim, Jong Soo Jurng, Min Young Song
  • Publication number: 20150133466
    Abstract: The invention provides methods for overriding cell cycle arrest in a tumor cell, which comprise inducing DNA damage in the cell, and contacting the cell with an amount of bosutinib or a bosutinib isomer effective to inhibit one or more kinase constituents of a DNA damage checkpoint pathway. The invention also provides novel bosutinib isomers, as well as compositions of the novel isomers and the bosutinib isomer 3,5-dichloro-4-methoxyaniline.
    Type: Application
    Filed: December 9, 2014
    Publication date: May 14, 2015
    Inventors: Timothy Yen, Neil Beeharry
  • Patent number: 9029108
    Abstract: A pulsed electric field method for the continuous extraction of oil and lipids from small aquatic plants, the method including providing a continuous flow of a slurry of small aquatic plants. A continuous flow of small aquatic plants is directed to a treatment zone. Pulsed electric fields are applied to the treatment zone to lyse cell walls of the small aquatic plants to enhance extraction of oil and lipids therefrom.
    Type: Grant
    Filed: September 15, 2010
    Date of Patent: May 12, 2015
    Assignee: Diversified Technologies, Inc.
    Inventors: Michael Alan Kempkes, Ian Roth, Marcel Pierre Joseph Gaudreau
  • Patent number: 9023787
    Abstract: The present invention relates to compounds and pharmaceutical compositions for treating cellular proliferative disorders, e.g., in patients having one or more p53-deficient cells, screening assays for identifying such compounds, and methods for treating such disorders.
    Type: Grant
    Filed: May 13, 2013
    Date of Patent: May 5, 2015
    Assignee: Massachusetts Institute of Technology
    Inventors: Michael B. Yaffe, Isaac A. Manke, Hans Christian Reinhardt
  • Publication number: 20150119448
    Abstract: The present invention relates to small RNAs, inhibitors thereof, inhibitors of enzymes producing thereof, and their use to modulate the response of a cell to a DNA damaging event. The invention concerns also a method to detect the presence or quantify DNA damage.
    Type: Application
    Filed: May 10, 2013
    Publication date: April 30, 2015
    Inventors: Fabrizio D'Adda Di Fagagna, Sofia Francia, Flavia Michelini, Francesca Rossiello
  • Patent number: 9018013
    Abstract: The present invention provides compositions and methods for the genetic manipulation of Algal cells. The compositions and methods allow enhanced transfer of genetic material into Algal cells and the cloning and selection of genetically modified cells. Expression of proteins encoded by the genetic material will be enhanced by the methods and compositions of the invention.
    Type: Grant
    Filed: August 13, 2012
    Date of Patent: April 28, 2015
    Assignee: Life Technologies Corporation
    Inventors: Farzad Haerizadeh, Todd Peterson, Wen Chen, Ewa Lis
  • Publication number: 20150110749
    Abstract: The technology described herein relates to methods, assays, and compositions relating to causing a cell to assume a more pluripotent state, e.g. without introducing foreign genetic material.
    Type: Application
    Filed: April 24, 2013
    Publication date: April 23, 2015
    Inventors: Charles A. Vacanti, Martin P. Vacanti, Koji Kojima, Haruko Obokata, Teruhiko Wakayama, Yoshiki Sasai, Masayuki Yamato
  • Patent number: 9011698
    Abstract: A method and devices comprise a low frequency high energy ultrasound system having at least one sonotrode projecting into a reactor vessel through which the liquid passes via at least one inlet orifice and at least one outlet orifice. To avoid cavitation at the sonotrode, in a close region of the oscillation-transducing sonotrode surface a pressure/amplitude combination close to or above the pressure-amplitude characteristic line is generated at which considerably reduced or no cavitation occurs and in the adjacent region in the vessel at least in a region and at least at times a pressure/amplitude combination is maintained below the pressure-amplitude characteristic line at which cavitation is generated. A device has an inlet orifice arranged such that the liquid impacts directly onto the oscillation-transducing sonotrode surface, and is shaped that in the close region of the oscillation-transducing sonotrode surface a pressure close to or above the pressure-amplitude characteristic line prevails.
    Type: Grant
    Filed: November 28, 2006
    Date of Patent: April 21, 2015
    Assignee: Dr. Hielscher GmbH
    Inventors: Harald Hielscher, Holger Hielscher, Thomas Hielscher
  • Publication number: 20150104844
    Abstract: A material comprising positively and negatively charged nanoparticles, wherein one of said nanoparticles contained a magnetically responsive element, are combined with a support molecule, which is a long natural or synthetic molecule or polymer to make a magnetic nanoparticle assembly. When the magnetic nanoparticle assembly is combined with cells, it will magnetize those cells. The magnetized cells can then be washed to remove the magnetic nanoparticle assembly and the magnetized cells manipulated in a magnetic field.
    Type: Application
    Filed: November 7, 2014
    Publication date: April 16, 2015
    Inventor: Glauco R. SOUZA
  • Publication number: 20150105286
    Abstract: In this invention, a novel protein interaction domain is provided along with several of its variants. This domain is involved in protein-protein interactions with the Bcl-2 family of proteins. It is named BLID (Bcl2 family of proteins Like Interaction Domain). Several BLID peptides that could be useful for discovery of drugs to help fight pathological states like cancer are presented.
    Type: Application
    Filed: October 7, 2014
    Publication date: April 16, 2015
    Inventor: Carlos Witte-Hoffmann
  • Patent number: 9005941
    Abstract: The present invention includes methods, devices and systems for isolating a nucleic acid from a fluid comprising cells. In various aspects, the methods, devices and systems may allow for a rapid procedure that requires a minimal amount of material and/or results in high purity nucleic acid isolated from complex fluids such as blood or environmental samples.
    Type: Grant
    Filed: September 4, 2014
    Date of Patent: April 14, 2015
    Assignee: Biological Dynamics, Inc.
    Inventors: Rajaram Krishnan, David Charlot, Eugene Tu, James McCanna, Lucas Kumosa, Paul D. Swanson, Robert Turner, Kai Yang, Irina Dobrovolskaya, David Liu
  • Patent number: 9005670
    Abstract: The present invention provides methods for treating cancer comprising inhibiting the activity of p300/CBP histone acetyltransferase (HAT). Also provided are p300/CBP HAT inhibitors for treating a subject having cancer. In addition, the present invention includes biomarkers for p300/CBP HAT inhibition, which are used to i) monitor the effectiveness of cancer therapy, and ii) identify anti-cancer agents for use in combination therapy.
    Type: Grant
    Filed: January 5, 2011
    Date of Patent: April 14, 2015
    Assignee: The Johns Hopkins University
    Inventors: Rhoda Myra Alani, Philip A. Cole, Gai Yan, Erin M. Bowers
  • Patent number: 9005604
    Abstract: A scaffold assembly and related methods of manufacturing and/or using the scaffold for stem cell culture and tissue engineering applications are disclosed which at least partially mimic a native biological environment by providing biochemical, topographical, mechanical and electrical cues by using an electroactive material. The assembly includes at least one layer of substantially aligned, electrospun polymer fiber having an operative connection for individual voltage application. A method of cell tissue engineering and/or stem cell differentiation uses the assembly seeded with a sample of cells suspended in cell culture media, incubates and applies voltage to one or more layers, and thus produces cells and/or a tissue construct.
    Type: Grant
    Filed: December 15, 2010
    Date of Patent: April 14, 2015
    Assignee: The United States of America as represented by the Administrator of the National Aeronautics and Space Administration
    Inventors: Lisa A. Scott-Carnell, Emilie J. Siochi, Nancy M. Holloway, Kam W. Leong, Karina Kulangara
  • Patent number: 8999714
    Abstract: Certain embodiments disclosed herein include, but are not limited to, at least one of compositions, methods, devices, systems, kits, or products regarding rejuvenation or preservation of stem cells. Certain embodiments disclosed herein include, but are not limited to, methods of modifying stem cells, or methods of administering modified stem cells to at least one biological tissue.
    Type: Grant
    Filed: October 12, 2012
    Date of Patent: April 7, 2015
    Assignee: The Invention Science Fund I, LLC
    Inventors: Roderick A. Hyde, Lowell L. Wood, Jr.
  • Patent number: 9000357
    Abstract: An apparatus and method to identify at least one component from a plurality of components in a fluid mixture, includes a first input channel containing the fluid mixture of components; at least one buffer input channel, into which at least one additional flow of buffer solution is introduced; a plurality of regions disposed at the other end of the apparatus, which are adapted to receive outputs of at least one selected component of the plurality of components, the selected component which is selectively removed from the first flow to one of the regions; a waste channel through which unselected components are removed from the first flow; a plurality of pumps connected to at least one reservoir, to control flow rates of the first flow and the additional flow(s); and a computer which controls a selection of one of the plurality of components from the fluid mixture.
    Type: Grant
    Filed: June 27, 2014
    Date of Patent: April 7, 2015
    Assignee: Premium Genetics (UK) Ltd.
    Inventors: Daniel Mueth, Joseph Plewa, Jessica Shireman, Amy Anderson, Lewis Gruber, Neil Rosenbaum
  • Publication number: 20150093365
    Abstract: Provided herein are compositions containing photoactivatable caged tamoxifen and tamoxifen derivative molecules. Also provided are kits containing one of these compositions and a light source. Also provided are methods of optically inducing nuclear translocation of a fusion protein containing a mammalian estrogen receptor ligand binding domain in a eukaryotic cell and methods of optically inducing recombination in a eukaryotic cell that include contacting a eukaryotic cell with at least one of these compositions. Also provided are methods of treating breast cancer in a subject that include administering a photoactivatable caged tamoxifen or tamoxifen derivative molecule to a subject having breast cancer.
    Type: Application
    Filed: March 14, 2013
    Publication date: April 2, 2015
    Inventors: Xin Lu, Sarit S. Agasti, Ronald A. DePinho, Ralph Weissleder
  • Publication number: 20150093367
    Abstract: A system and method are provided that use the meridian flow defined in traditional Chinese medicine to target specific regions of the body to treat certain health conditions. A cellular mixture is injected into the body via an acupoint on a particular meridian in order to deliver the mixture to an organ or body region associated with the particular meridian. The cellular mixture can be a stem cell/PRP mixture.
    Type: Application
    Filed: September 29, 2014
    Publication date: April 2, 2015
    Inventor: BILL AKPINAR
  • Publication number: 20150093416
    Abstract: Autologous anti-cancer vaccines and methods of manufacture and treatment are provided, including expansion of individual patient-derived tumor cells in an immune-compromised animal(s) to attain, quantitatively and qualitatively, sufficient material for efficacious vaccine production and utilization, to elicit an immune response against micrometastases and/or recurrence in the individual patient following tumor excision.
    Type: Application
    Filed: September 25, 2014
    Publication date: April 2, 2015
    Inventor: Michael G. Hanna, JR.
  • Patent number: 8993292
    Abstract: Methods are provided for differential extraction of DNA from at least two different cell types. Systems for carrying out the differential extraction methods are also provided. A kit is also provided for differential extraction of DNA from at least two different cell types using a multi-compartment container.
    Type: Grant
    Filed: May 8, 2008
    Date of Patent: March 31, 2015
    Assignee: Applied Biosystems LLC
    Inventor: Yingjie Liu
  • Patent number: 8986607
    Abstract: A validatable method for determining a photochemically effective dose for inactivating pathogens in a fluid sample is described herein. In particular, the instant invention covers methods for determining a photochemically effective dose sufficient to inactivate pathogens in a biological sample while leaving biologically active substances of interest unaffected. A batch irradiation reactor effective for inactivating pathogens in biological samples is also described.
    Type: Grant
    Filed: February 27, 2004
    Date of Patent: March 24, 2015
    Assignees: Baxter International Inc., Baxter Healthcare SA
    Inventors: Heinz Anderle, Peter Matthiessen, Hans-Peter Schwarz, Peter Turecek, Thomas Kreil, Daniel R. Boggs
  • Publication number: 20150072394
    Abstract: The present disclosure provides opsins, including variant opsins with increased activity and/or increased trafficking to the plasma membrane. The opsins are useful in therapeutic and screening applications, which are also provided.
    Type: Application
    Filed: December 12, 2012
    Publication date: March 12, 2015
    Inventors: Karl Deisseroth, Feng Zhang
  • Patent number: 8975065
    Abstract: Methods and devices of performing reactions for which presence of light is desirable are provided. Biological or chemical materials such as algae are put in a chamber shaped as a meandering fluid channel. The algae can be combined with biomass such as human or animal waste and then subject to light, such as natural light or light coming from a LED, to produce fuel. Production of fuel can be optimized by controlling the height-to-width ratio of the channels.
    Type: Grant
    Filed: July 23, 2007
    Date of Patent: March 10, 2015
    Assignee: California Institute of Technology
    Inventors: George Maltezos, Axel Scherer