Enzyme Or Microbial Cell Is Immobilized On Or In An Inorganic Carrier Patents (Class 435/176)
  • Patent number: 11014069
    Abstract: The present invention relates to a method to improve chromatography beads. More closely, the invention relates to a novel method for production of dextran-containing porous media and chromatography media produced with this method. In the method, the chromatography media is subjected to dextranase-treatment leading to improved pressure-flow properties of the media.
    Type: Grant
    Filed: October 28, 2016
    Date of Patent: May 25, 2021
    Assignee: Cytiva BioProcess R&D AB
    Inventors: Nils Norrman, Mattias Algotsson, Stefan D. Eriksson
  • Patent number: 10859477
    Abstract: A composition for use in bioseparation. The composition includes a plurality of hollow particles having a siliceous surface. The composition further includes a surface-modifying agent bonded to the hollow particles. The surface-modifying agent includes a binding segment and a reactive segment. The binding segment includes a silyl group and the reactive segment includes a reactive nitrogen group.
    Type: Grant
    Filed: January 24, 2017
    Date of Patent: December 8, 2020
    Assignee: 3M Innovative Properties Company
    Inventors: Masayuki Nakamura, Naota Sugiyama, Cynthia D. Zook, Stephen E. Amos, Jerald K. Rasmussen, Nicole M. Gryska
  • Patent number: 10816553
    Abstract: Disclosed herein are formulations, substrates, and arrays. Also disclosed herein are methods for manufacturing and using the formulations, substrates, and arrays. Also disclosed are methods for identifying peptide sequences useful for diagnosis and treatment of disorders, and methods for using the peptide sequences for diagnosis and treatment of disorders, e.g., celiac disorder. In certain embodiments, substrates and arrays comprise a porous layer for synthesis and attachment of polymers or biomolecules.
    Type: Grant
    Filed: February 17, 2014
    Date of Patent: October 27, 2020
    Assignee: Vibrant Holdings, LLC
    Inventors: John J. Rajasekaran, Vasanth Jayaraman, Tianhao Wang, Kang Bei, Hari Krishnan Krishnamurthy
  • Patent number: 10767024
    Abstract: Soft actuators are fabricated from materials that enable the actuators to be constructed with an open-celled architecture such as an interconnected network of pore elements. The movement of a soft actuator is controlled by manipulating the open-celled architecture, for example inflating/deflating select portions of the open-celled architecture using a substance such as compressed fluid.
    Type: Grant
    Filed: April 9, 2015
    Date of Patent: September 8, 2020
    Assignee: Cornell University
    Inventors: Robert F. Shepherd, Benjamin C. MacMurray, Huichan Zhao
  • Patent number: 10669520
    Abstract: Disclosed herein is an improved automatic sampling and biochemical monitoring system for a microbial or cell culture or a biochemical reaction in a bioreactor.
    Type: Grant
    Filed: April 20, 2018
    Date of Patent: June 2, 2020
    Inventors: Timothy Ray Ho, Lewis Ho
  • Patent number: 10662400
    Abstract: The present invention provides a microcapsule composition produced by crosslinking of a polybranched polyamine and a small amine, which is used for stabilizing detergent components.
    Type: Grant
    Filed: March 25, 2015
    Date of Patent: May 26, 2020
    Assignee: Novozymes A/S
    Inventors: Amra Tihic Rasmussen, Kim Bruno Andersen, Katarina Larson, Lotte Elisabeth Nissen, Martin Noerby, Ole Simonsen, Tue Rasmussen
  • Patent number: 10605705
    Abstract: The present invention is directed to the use of silicic acid to transform biological materials, including cellular architecture into inorganic materials to provide biocomposites (nanomaterials) with stabilized structure and function. In the present invention, there has been discovered a means to stabilize the structure and function of biological materials, including cells, biomolecules, peptides, proteins (especially including enzymes), lipids, lipid vesicles, polysaccharides, cytoskeletal filaments, tissue and organs with silicic acid such that these materials may be used as biocomposites. In many instances, these materials retain their original biological activity and may be used in harsh conditions which would otherwise destroy the integrity of the biological material. In certain instances, these biomaterials may be storage stable for long periods of time and reconstituted after storage to return the biological material back to its original form.
    Type: Grant
    Filed: April 11, 2018
    Date of Patent: March 31, 2020
    Assignees: National Technology & Engineering Solutions of Sandia, LLC, STC.UNM
    Inventors: Bryan J. Kaehr, C. Jeffrey Brinker, Jason L. Townson
  • Patent number: 10539571
    Abstract: A method of proteolyzing a protein, including immobilizing a protein in at least one pore of a porous body, and contacting the protein immobilized in the pore and a protease immobilized on a solid surface such that the protease selectively accesses a site of the protein and proteolyzes the protein at the site.
    Type: Grant
    Filed: March 8, 2016
    Date of Patent: January 21, 2020
    Assignee: SHIMADZU CORPORATION
    Inventors: Takashi Shimada, Noriko Fukao, Chikage Aoki, Taka-Aki Sato
  • Patent number: 10517302
    Abstract: The present disclosure addresses biologically active formulations for agricultural and other applications that comprise a solid growth substrate that defines an open cell matrix and an active population of one or more microorganisms adhered thereto. The formulation is configured to be applied directly to a plant growth environment and does not require additional isolation and/or processing steps that would separate the microorganisms from the solid growth substrate prior to deployment. The disclosure also addresses related methods.
    Type: Grant
    Filed: February 17, 2015
    Date of Patent: December 31, 2019
    Assignee: LiveGrow Bio LLC
    Inventors: Alan Stuart Wicks, Grigoriy E. Pinchuk
  • Patent number: 10520884
    Abstract: A cartridge includes a cleaning member configured to retain specific particles having a smaller equivalent sphere diameter than toner particles at a contact region. The specific particles contain an organosilicon polymer having a partial structure represented by R—SiO3/2, wherein R represents an alkyl group having 1 to 6 carbon atoms. The atomic concentration dSi of silicon in the specific particles satisfies 1.0 atomic %?dSi?29.0 atomic % when the total atomic concentration of silicon, oxygen, and carbon in the specific particles is measured to be 100.0 atomic % by electron spectroscopy for chemical analysis (ESCA). Also, the specific particles satisfy L2/L3?3/4 or L2/L3?4/3 when assuming that the specific particles have three lengths L1, L2, and L3 in three axis directions in a three-dimensional coordinate system, wherein L1 is the longest one of the three lengths, in an average value of a unit volume.
    Type: Grant
    Filed: December 11, 2018
    Date of Patent: December 31, 2019
    Assignee: Canon Kabushiki Kaisha
    Inventors: Yasukazu Ikami, Yoshihiro Mitsui, Takuya Oka
  • Patent number: 10478167
    Abstract: Medical adhesive applicators are disclosed which allow medical adhesives to have long storage life and be easily dispensed in a controlled manner. In one embodiment, the applicator has a frangible glass vial with the medical adhesive. The frangible glass vial is held in a plastic enclosure with a dispensing tip. Attached to the plastic enclosure is a squeeze tube capable of imparting air pressure into the enclosure. To dispense medical adhesive quickly and with precision, one simply breaks the frangible glass vial and pushes on the squeeze tube. In another embodiment, the applicator has a proximal chamber containing medical adhesive, a distal chamber for dispensing medical adhesive and one or more rupturable membranes between them. To achieve long shelf life, the applicator chambers and membrane(s) are formed from fluorinated plastic. To dispense medical adhesive, one ruptures the membrane(s) and pushes on the sides of the proximal chamber.
    Type: Grant
    Filed: April 3, 2018
    Date of Patent: November 19, 2019
    Assignee: Rousseau Research, Inc.
    Inventor: Joseph D. Russo
  • Patent number: 10386365
    Abstract: Methods and sensors for detection and quantification of one or more analyte in a test sample are described. A response profile of an ion sensor to a control sample of a known interrogator ion is determined. The ion sensor is exposed to a test sample then to a second sample comprising the known interrogator ion, and a test sample response profile of the ion sensor is determined. One or more test sample sensor response profiles are compared with one or more control sensor response profiles for detecting, identifying, and quantifying one or more analytes in the test sample.
    Type: Grant
    Filed: December 7, 2016
    Date of Patent: August 20, 2019
    Assignee: NANOHMICS, INC.
    Inventors: Steve M. Savoy, Kyle W. Hoover, Chris W. Mann, Daniel R. Mitchell, Jeremy J. John, Alexander P. Greis
  • Patent number: 10316284
    Abstract: The invention concerns a bioreactor obtained by compressing a mixture of an enzyme, a conductor and chitosan. The conductor can consist of carbon nanotubes. This bioreactor can be produced according to the following steps: preparing a mixture of powders in which the proportion of enzyme powder relative to a carbon nanotube powder is of the order of 50/50 by weight; preparing a viscous solution of chitosan in a ratio of 5 to 15 (in mg) of chitosan to 0.75 to 1.25 (in ml) of acetic acid diluted to 0.4 to 0.6% by volume in water; adding the viscous chitosan to the mixture of powders in a proportion be weight of 3 to 5 of powder to 5 to 10 of chitosan; carrying out a first compression followed by light grinding; carrying out a second compression to produce a pellet; and drying at ambient temperature.
    Type: Grant
    Filed: March 23, 2015
    Date of Patent: June 11, 2019
    Assignee: Universite Grenoble Alpes
    Inventors: Sarra El Ichi, Donald K. Martin, Philippe Cinquin, Abdelkader Zebda
  • Patent number: 10112916
    Abstract: Methods of converting lignocellulosic derivatives form 5-hydroxymethyl furfural (HMF) in ionic liquids are provided herein. Glucose is enzymatically converted to fructose by isomerization with glucose isomerase and a borate salt. Conversion of fructose to HMF includes dehydration of fructose using ionic liquids or ionic liquids with an acid catalyst to achieve high yield of HMF.
    Type: Grant
    Filed: December 16, 2015
    Date of Patent: October 30, 2018
    Assignees: The Regents of the University of California, Virdia Inc., National Technology & Engineering Solutions of Sandia, LLC
    Inventors: Noppadon Sathitsuksanoh, Philip Travisano, III, Anthe George, Robert Jansen, Blake A. Simmons, Seema Singh
  • Patent number: 10100163
    Abstract: The present invention provides crosslinked epoxy-functional copolymer films and microarrays built from the crosslinked epoxy-functional copolymer films. Microarrays incorporating the copolymers include a substrate on which a film of the crosslinked epoxy-functional copolymer is disposed and target molecules bound to the copolymer film. The crosslinked polymer films are well-suited for use as scaffolds for target molecules in microarrays because they provide a high density of binding sites for the target molecules, are mechanically stable, and may be coated onto a wide range of substrates.
    Type: Grant
    Filed: May 28, 2015
    Date of Patent: October 16, 2018
    Assignee: Wisconsin Alumni Research Foundation
    Inventor: Padma Gopalan
  • Patent number: 10066323
    Abstract: Methods of making polycationic nanofibers by grafting cationic polymers onto electrospun neutral nanofibers and polycationic nanofibers produced by the methods are provided herein. In addition, methods of using the polycationic nanofibers to reduce inflammation, to adsorb anionic compounds such as heparin or nucleic acids, to inhibit the growth of microbes or inhibit the formation of a biofilm are also provided. The polycationic nanofibers may be in a mesh and may be included in a medical device, wound dressing, bandage, or as part of a graft.
    Type: Grant
    Filed: April 16, 2015
    Date of Patent: September 4, 2018
    Assignee: Duke University
    Inventors: Bruce A. Sullenger, Hemraj A. Juwarker, Kam W. Leong, Jennifer Gamboa Jackman
  • Patent number: 9926383
    Abstract: The present invention relates to a method of producing a solid coated carrier carrying biological material. Furthermore, the invention relates to a solid coated carrier to which biological material is attached and uses of the solid coated carrier for the preparation of a medical product. Moreover, the invention provides a method for the contacting, filtration or cleaning of blood, lymph or liquor cerebrospinalis of a patient, a method for the diagnosis of a disease and a diagnostic composition.
    Type: Grant
    Filed: May 7, 2007
    Date of Patent: March 27, 2018
    Assignee: LEUKOCARE AG
    Inventors: Stefan Margraf, Martin Scholz
  • Patent number: 9765296
    Abstract: An object of the present invention is to provide a method capable of inexpensively and conveniently preparing cells having pluripotency and a very low risk of tumorigenic transformation.
    Type: Grant
    Filed: September 3, 2014
    Date of Patent: September 19, 2017
    Assignee: OTSUKA PHARMACEUTICAL FACTORY, INC.
    Inventors: Risa Okairi, Masuhiro Nishimura, Tamaki Wada
  • Patent number: 9717688
    Abstract: A process is provided for releasably encapsulating a biological entity. The process comprises combining a solution of a surfactant in a non-polar solvent with a precursor material and the biological entity to form an emulsion. The emulsion comprises a polar phase dispersed in a non-polar phase, wherein the polar phase comprises the biological entity. The particles comprising the biological entity are then formed from the polar phase.
    Type: Grant
    Filed: February 3, 2015
    Date of Patent: August 1, 2017
    Assignee: AUSTRALIAN NUCLEAR SCIENCE & TECHNOLOGY ORGANISATION
    Inventors: Kim Suzanne Finnie, David Jacques, Christophe Jean Alexandre Barbe, Linggen Kong
  • Patent number: 9678080
    Abstract: Multi-biotinylated reactants are provided which can be used in divalent complexes for various applications such as colocalization, labeling, immobilization, and purification. Methods for constructing, purifying, and using the bis-biotinylated reactants are also provided. In certain embodiments, two bis-biotinylated reactants are bound to a single streptavidin tetramer to provide a complex having a 1:1 stoichiometry with respect to the bis-biotinylated reactants.
    Type: Grant
    Filed: June 12, 2014
    Date of Patent: June 13, 2017
    Assignee: Pacific Biosciences of California, Inc.
    Inventors: Erik Miller, Satwik Kamtekar, Keith Bjornson, Jeremiah Hanes
  • Patent number: 9677065
    Abstract: Methods are provided for the encapsulation of living cells with a silica glass layer. The methods comprise adding a sol solution to cells in buffered media, forming a sol-gel layer on the cells and then diluting the media or removing the cells from the media.
    Type: Grant
    Filed: July 21, 2010
    Date of Patent: June 13, 2017
    Assignee: PURDUE RESEARCH FOUNDATION
    Inventors: David Benjamin Jaroch, Jenna Leigh Rickus
  • Patent number: 9456777
    Abstract: Devices, systems, and methods for assessing microbiota of skin are described which include: a skin-covering material with an inner surface conforming in shape to a topography of a skin surface and including a plurality of specific microbe-binding elements; an image capture device to capture an image of the inner surface of the skin-covering material and to transform the image into a digital output including information associated with at least one property and a spatial distribution of at least one type of microbe bound to the specific microbe-binding elements; and a computing device including circuitry configured to receive the digital output from the image-capture device, compare the at least one property of the at least one type of microbe bound to the specific microbe-binding elements with a database of reference microbe properties, and generate a digital profile including the at least one property and the spatial distribution of the at least one type of microbe bound to the specific microbe-binding eleme
    Type: Grant
    Filed: August 23, 2013
    Date of Patent: October 4, 2016
    Assignee: Elwha LLC
    Inventors: Mahalaxmi G. Bangera, Michael H. Baym, Roderick A. Hyde, Jordin T. Kare, Eric C. Leuthardt, Gary L. McKnight, Tony S. Pan, Katherine E. Sharadin, Elizabeth A. Sweeney, Clarence T. Tegreene, Lowell L. Wood, Jr.
  • Patent number: 9434973
    Abstract: Functionalized substrate materials, for example inorganic particles and/or synthetic polymeric particles, are used to enhance bioprocesses such as saccharification and fermentation.
    Type: Grant
    Filed: September 23, 2015
    Date of Patent: September 6, 2016
    Assignee: XYLECO, INC.
    Inventors: Marshall Medoff, Thomas Craig Masterman, Harrison Medoff
  • Patent number: 9393316
    Abstract: A peptide targeted conjugate is disclosed. The conjugate comprises: (a) an isolated or a synthetic targeting peptide of less than 15 amino acid residues in length, comprising an amino acid sequence having at least 90% identity to a sequence selected from the group consisting of SEQ ID NOs: 1, 2 and 3; and (b) a component, to which the targeting peptide is conjugated, the component being selected from the group consisting of a drug delivery vehicle, an anti-cancer drug, a micelle, a nanoparticle, liposomes, a polymer, a lipid, an oligonucleotide, a peptide, a polypeptide, a protein, a cell, an imaging agent, and a labeling agent. The conjugate may further comprise an anti-cancer agent encapsulated within the drug delivery vehicle. Compositions and methods of treating cancer are also disclosed.
    Type: Grant
    Filed: September 28, 2014
    Date of Patent: July 19, 2016
    Assignee: ACADEMIA SINICA
    Inventors: Han-Chung Wu, Chien-Hsun Wu
  • Patent number: 9390312
    Abstract: Devices, systems, and methods for assessing microbiota of skin are described which include: a skin-covering material with an inner surface conforming in shape to a topography of a skin surface and including a microbe-capture region; an image-capture device to capture a least one image of the inner surface of the skin-covering material and to transform the captured at least one image into a digital output including information associated with at least one property and a spatial distribution of at least one type of microbe bound to the microbe-capture region; and a computing device including circuitry configured to receive the digital output from the image-capture device, compare the at least one property of the at least one type of microbe with a database of reference microbe properties, and generate a digital profile including the at least one property and the spatial distribution of the at least one type of microbe bound to the microbe-capture region of the skin-covering material.
    Type: Grant
    Filed: August 23, 2013
    Date of Patent: July 12, 2016
    Assignee: Elwha LLC
    Inventors: Mahalaxmi G. Bangera, Michael H. Baym, Roderick A. Hyde, Jordin T. Kare, Eric C. Leuthardt, Gary L. McKnight, Tony S. Pan, Katherine E. Sharadin, Elizabeth A. Sweeney, Clarence T. Tegreene, Lowell L. Wood, Jr.
  • Patent number: 9303256
    Abstract: The present invention relates to the immobilization of enzymes by adsorbing enzymes, a polyfunctional amine and a cross-linking agent onto a particulate porous carrier in a mixer apparatus or in a fluid bed apparatus.
    Type: Grant
    Filed: October 2, 2006
    Date of Patent: April 5, 2016
    Assignee: Novozymes A/S
    Inventors: Isabelle Mazeaud, Poul Borge Rosenius Poulsen, Morten Wurtz Christensen, Jesper Brask
  • Patent number: 9181541
    Abstract: The present invention provides methods for the cultivation of the Methylobacterium genus of bacteria. In particular the method provides methods for the efficient and inexpensive cultivation of these bacteria. Additionally, the invention provides methods for the utilization of these bacterial cultures to improve plant agriculture.
    Type: Grant
    Filed: May 31, 2013
    Date of Patent: November 10, 2015
    Assignee: NewLeaf Symbiotics, Inc.
    Inventor: Gregg Bogosian
  • Patent number: 9168141
    Abstract: Disclosed are medical prosthetic device or medical implants which exhibit improved biocompatibility. The devices or implants include a metal material, e.g., titanium, in which the metal surfaces are coated with a corresponding hydride material that contains one or more biomolecule substance. The biomolecule substance may contain one or more biologically active molecules, e.g., bio-adhesives, biopolymers, blood proteins, enzymes, extracellular matrix proteins, extracellular matrix biomolecules, growth factors and hormones, peptide hormones, deoxyribonucleic acids, ribonucleic acids, receptors, inhibitors, drugs, biologically active anions and cations; vitamins; adenosine monophosphate (AMP), adenosine diphosphate (ADP) or adenosine triphosphate (ATP), marker biomolecules, amino acids, fatty acids, nucleotides (RNA and DNA bases), or sugars.
    Type: Grant
    Filed: November 17, 2006
    Date of Patent: October 27, 2015
    Assignee: Astra Tech AB
    Inventors: Jan Eirik Ellingsen, Staale Petter Lyngstadaas
  • Publication number: 20150140604
    Abstract: Disclosed in the present invention are an immobilized cycloaliphatic peptide acyltransferase and a preparation method and use thereof. The cycloaliphatic peptide acyltransferase is immobilized on a carrier; the cycloaliphatic peptide acyltransferase is derived from natural or artificial mutants or variants thereof, or can be obtained by introducing a foreign cyclic acyltransferase gene and transforming thereafter; the material of the carrier is selected from an inorganic carrier or a polypropylene resin carrier. Also disclosed in the present invention are the preparation method for the immobilized cycloaliphatic peptide acyltransferase and uses thereof.
    Type: Application
    Filed: May 10, 2013
    Publication date: May 21, 2015
    Inventors: Shidong Liu, Zhaoli Zhang, Xiaoming Ji
  • Publication number: 20150118729
    Abstract: Disclosed herein are compositions and methods for programming a cell. The compositions include a substrate and a cell adhesion agent. The substrate includes a surface having a micropatterned object and the cell adhesion agent is immobilized within a first area defined by the micropatterned object.
    Type: Application
    Filed: October 29, 2014
    Publication date: April 30, 2015
    Inventors: Kristopher A. Kilian, Junmin Lee, Yanfen Li
  • Patent number: 8992845
    Abstract: A method for separating CO2 from gas mixtures uses a slurried media impregnated with buffer compounds and coating the solid media with a catalyst or enzyme that promotes the transformation of CO2 to carbonic acid. Buffer sorbent pebbles with a catalyst or enzyme coating are provided for rapid separation of CO2 from gas mixtures.
    Type: Grant
    Filed: February 4, 2013
    Date of Patent: March 31, 2015
    Assignee: Lawrence Livermore National Security, LLC
    Inventor: Roger D. Aines
  • Patent number: 8969027
    Abstract: The present invention provides a diagnostic reagent or assay for assessing the activity of a protease in vivo or in vitro and methods of detecting the presence of a cancerous or precancerous cell. The assays are comprised of two particles linked via an oligopeptide linkage that comprises a consensus sequence specific for the target protease. Cleavage of the sequence by the target protease can be detected visually or using various sensors, and the diagnostic results can be correlated with cancer prognosis.
    Type: Grant
    Filed: March 3, 2009
    Date of Patent: March 3, 2015
    Assignee: Kansas State University Research Foundation
    Inventors: Stefan H. Bossmann, Deryl L. Troyer, Matthew T. Basel
  • Patent number: 8962301
    Abstract: A biochip and a method for manufacturing the same are provided. The biochip can increase a surface area on which biomaterials can be immobilized and improve efficiency of reaction. The biochip includes: a substrate; a coating layer formed on the substrate; and a nanoparticle immobilized on the substrate by the coating layer, a material to be bound to a target material being attached on a surface of the nanoparticle.
    Type: Grant
    Filed: October 4, 2011
    Date of Patent: February 24, 2015
    Assignee: Intellectual Discovery Co., Ltd.
    Inventor: Nae Rym Lee
  • Patent number: 8956839
    Abstract: The present invention generally relates to immobilized biocatalysts or immobilized enzymes for use in carbon capture and sequestration technology. Thiol-ene chemistry is used to couple a biocatalyst, particularly carbonic anhydrase, to a substrate including a substrate, a solid support, a microparticle, a nanoparticle, or a combination thereof.
    Type: Grant
    Filed: June 6, 2013
    Date of Patent: February 17, 2015
    Assignee: Akermin, Inc.
    Inventors: Brett M. Rambo, John Reardon, Aleksey Zaks
  • Patent number: 8940494
    Abstract: Methods of assaying the leukocyte adhesion cascade (LAC) and monitoring leukocyte rolling, adhesion, and/or migration can be implemented with an apparatus that includes an idealized microvascular network (IMN) of one or more interconnected idealized flow channels in fluid communication through a porous wall with a tissue space (e.g., idealized tissue space). The methods of assaying the LAC can be implemented with means for quantifying modulation of the leukocyte adhesion cascade. Methods of assaying the LAC can be implemented with the device and one or more active agents to monitor leukocyte rolling, adhesion, and/or migration in the presence of absence of the active agent. Migration can be through the idealized flow channels, through the porous wall, and/or into the tissue space.
    Type: Grant
    Filed: January 16, 2013
    Date of Patent: January 27, 2015
    Assignee: CFD Research Corporation
    Inventors: Balabhaskar Prabhakarpandian, Kapil Pant
  • Publication number: 20150017699
    Abstract: The present invention is generally directed to internally functionalized graphene substrates, methods of making such substrates and methods of using such substrates. In one aspect, the present invention is a graphene substrate. The substrate comprises edge and non-edge regions. Organic or inorganic molecules are bound to the non-edge regions of the substrate, and the organic or inorganic molecules are present on the substrate edges at a population greater than about one molecule per 10,000 nm2.
    Type: Application
    Filed: July 9, 2014
    Publication date: January 15, 2015
    Applicant: NANOTECH BIOMACHINES, INC.
    Inventors: Jeffrey Alan McKinney, William Emerson Martinez
  • Patent number: 8911980
    Abstract: The present invention provides a method for producing a ground product of a lipase powder composition comprising an immobilized lipase in which a lipase derived from Thermomyces sp. is immobilized to a silica carrier and a hydrophilic powder other than a silica carrier, the method comprising the steps of wetting the hydrophilic powder with oil; separating the hydrophilic powder wetted with oil from the oil to obtain an oil-containing hydrophilic powder; mixing the oil-containing hydrophilic powder and the immobilized lipase at a ratio of 0.45:1 to 0.85:1 based on mass of the hydrophilic powder and the immobilized lipase before oil wetting; and grinding the obtained mixture, the steps being conducted in the above order.
    Type: Grant
    Filed: March 26, 2010
    Date of Patent: December 16, 2014
    Assignee: The Nisshin Oillio Group, Ltd.
    Inventors: Junko Suzuki, Yoshie Yamauchi, Yuko Toyama, Satoshi Negishi
  • Publication number: 20140363872
    Abstract: Materials and Methods are provided for producing cell-surface directed, non-naturally occurring, bioinorganic membranes for association with the cell surfaces of living cells. The methods comprise exposing a cell to an acidic biomineralization buffer environment for cell-mediated deposition of the biomineral membrane onto the surface of the cell. The methods also comprise attaching a peptide, having a net positive charge under the acidic conditions, to the cell surface for serving as a template in directing the cell-mediated deposition of the biomineral membrane onto the surface of the cell.
    Type: Application
    Filed: June 17, 2014
    Publication date: December 11, 2014
    Inventors: David Benjamin Jaroch, Jenna Leigh Rickus
  • Patent number: 8895046
    Abstract: Adult autologous stem cells cultured on a porous, three-dimensional tissue scaffold-implant for bone regeneration by the use of a hyaluronan and/or dexamethasone to accelerate bone healing alone or in combination with recombinant growth factors or transfected osteogenic genes. The scaffold-implant may be machined into a custom-shaped three-dimensional cell culture system for support of cell growth, reservoir for peptides, recombinant growth factors, cytokines and antineoplastic drugs in the presence of a hyaluronan and/or dexamethasone alone or in combination with growth factors or transfected osteogenic genes, to be assembled ex vivo in a tissue incubator for implantation into bone tissue.
    Type: Grant
    Filed: October 9, 2007
    Date of Patent: November 25, 2014
    Assignee: Zimmer, Inc.
    Inventors: Zou Xuenong, Haisheng Li, Cody Bunger
  • Patent number: 8889389
    Abstract: The present invention relates to an efficient and economical process for producing a protein A-like protein. Hosts such as Escherichia coli and Bacillus subtilis have been used in the production of a protein A-like protein using a genetic recombination technique and however, their low productivity has been a big cause of high cost. Thus, it has been desired strongly to immediately establish a technique enabling the inexpensive, large-scale production of a protein A-like protein using recombinant DNA techniques other than Escherichia coli and Bacillus subtilis. The present invention provides a process for producing a protein A-like protein in large amounts, for example, a process comprising allowing a recombinant Brevibacillus genus bacterium to express and secrete the protein in large amounts into a culture solution and separating and collecting the accumulated protein A-like protein from the culture solution.
    Type: Grant
    Filed: October 29, 2013
    Date of Patent: November 18, 2014
    Assignee: Kaneka Corporation
    Inventors: Akihiko Kosugi, Kazuyoshi Yajima
  • Patent number: 8883441
    Abstract: A method for detecting and counting the microorganisms in a sample is described. The method comprises: a) selectively enriching the microorganism sought in the sample, b) inducing or activating at least one enzymatic activity of the microorganism, c) immunomagnetically concentrating the microorganism, d) fluorescently labeling the microorganism, and e) detecting and analyzing the fluorescence making possible the numeration or counting of the microorganisms sought by flow cytometry, filtration cytometry or fluorescence microscopy.
    Type: Grant
    Filed: October 1, 2003
    Date of Patent: November 11, 2014
    Assignee: Metis Biotechnologies
    Inventors: Bruno Vedrine, Aline Lachaise, Vincent Carre
  • Publication number: 20140322785
    Abstract: The invention provides methods for rapid, continuous generation of cells and cell products using magnetically stabilized three-dimensional tissue culture. The invention also pertains to a continuous flow self-regulating closed system bioreactor system for magnetically stabilized three-dimensional tissue culture. The methods described here do not use traditional solid scaffolding for cell culture.
    Type: Application
    Filed: May 9, 2014
    Publication date: October 30, 2014
    Applicant: Worcester Polytechnic Institute
    Inventors: Christopher R. Lambert, W. Grant McGimpsey, Raymond L. Page, Tanja Dominko
  • Publication number: 20140323589
    Abstract: The invention relates to a method for the preparation of composite silica alcogels, aerogels and xerogels, comprising i) providing a reaction mixture comprising at least the following: silane reagent, base catalyst, gelation retarding additive, aqueous/organic solvent mixture, guest particle, ii) agitating the reaction mixture as necessary and sufficient until achieving the viscosity where the spontaneous movement of the guest particles does not occur anymore; and iii) shaping the material obtained to a desired shape during or after step ii); then iv) drying, if desired The method according to the invention is also useful in continuous manufacturing technology, and the invention provides an apparatus for applying the method. The invention further provides novel composite silica alcogels, aerogels or xerogels obtainable by the method according to the invention.
    Type: Application
    Filed: October 26, 2012
    Publication date: October 30, 2014
    Applicant: Debreceni Egyetem
    Inventors: István Lázár, István Fábián
  • Patent number: 8871482
    Abstract: Functionalized substrate materials, for example inorganic particles and/or synthetic polymeric particles, are used to enhance bioprocesses such as saccharification and fermentation.
    Type: Grant
    Filed: May 9, 2013
    Date of Patent: October 28, 2014
    Assignee: Xyleco, Inc.
    Inventors: Marshall Medoff, Thomas Craig Masterman, Harrison Medoff
  • Publication number: 20140308728
    Abstract: A biomolecule immobilization substrate comprising a titania nanotube is provided. Stable undercoordinated titanium sites on the surface of titanium dioxide nanotubes provide for the binding of biomolecules in multiple layers and aggregates. Corresponding methods of immobilizing and storing biomolecules are provided. Enzymatic or other biological activities of titania nanotube bound biomolecules can be preserved or enhanced.
    Type: Application
    Filed: April 10, 2014
    Publication date: October 16, 2014
    Applicant: The University of North Carolina at Chapel Hill
    Inventors: Jacob H. Forstater, Alfred Kleinhammes, Yue Wu
  • Publication number: 20140309126
    Abstract: The present disclosure provides a peptide including one or more amino acid sequence selected from a group consisting of SEQ ID NO 1 and SEQ ID NO 2 and binding specifically to a graphitic material, a phage including same, and a graphitic material including a graphitic surface on which the peptide or the phage is arranged.
    Type: Application
    Filed: February 4, 2014
    Publication date: October 16, 2014
    Applicant: Korea Institute of Science and Technology
    Inventors: Hyunjung YI, Ki Young LEE, Chaun JANG, Joonyeon CHANG
  • Patent number: 8859249
    Abstract: A method for producing an iron (III) ion from the acidic solution containing an iodide ion and an iron (II) ion efficiently and stably is provided. The method including performing the following steps (a)-(b), repeatedly and continuously: (a) a step wherein the iron (II) ion in the acidic solution containing the iodide ion and the iron (II) ion is oxidized into iron (III) ion in a reactor using a microbes immobilizing carrier to which iron oxidizing microbes attached; (b) a step wherein sedimentation of the solution obtained in the step (a) is performed in a sedimentation tank to obtain the solution containing the iron (III) ion and concurrently the sediment of the microbes immobilizing carrier to which the iron oxidizing microbes have attached is recovered and then reintroduced into the reactor in the step (a).
    Type: Grant
    Filed: March 13, 2012
    Date of Patent: October 14, 2014
    Assignee: JX Nippon Mining & Metals Corporation
    Inventors: Kenichi Kuwano, Akira Miura
  • Publication number: 20140302130
    Abstract: The present invention provides compositions for encapsulation of biomaterials in a silica-matrix. The present invention also provides methods of making silica-matrix encapsulated biomaterials, and to methods of using silica-matrix encapsulated biomaterials. In one embodiment, the present invention provides a method of encapsulating mammalian cells in a silica-matrix while maintaining metabolic activity. In another embodiment, the present invention provides a method of purifying cancer cells using a silica-matrix.
    Type: Application
    Filed: November 7, 2012
    Publication date: October 9, 2014
    Inventors: Eduardo Reategui, Lisa Kasinkas, Alptekin Aksan
  • Patent number: 8846359
    Abstract: A method of regenerating an enzymatic catalyst arranged in a reactor includes a mineral support based on metal oxide and at least one enzyme, wherein it contains at least one step of detachment of the spent enzymes by solvation by scavenging of the catalyst using at least one ionic surfactant, and at least one step of re-attachment of active enzymes by scavenging of the purified support with at least one solution of active enzymes, the two steps being performed in situ within the reactor.
    Type: Grant
    Filed: September 20, 2012
    Date of Patent: September 30, 2014
    Assignee: Total Marketing Services
    Inventors: Daniel Thomas, Sylviane Pulvin, Lotfi Hedhli, Samuel Djelassi
  • Patent number: 8834953
    Abstract: The present invention relates to a protein-binding material including a mesoporous silica and a method for selectively separating and purifying using the protein-binding material. More specifically, the present invention relates to a method of preparing a magnetic mesoporous silica responding to a magnetic field by adsorbing a precursor of a transition metal or its ion, such as an iron (Fe) precursor, onto a mesoporous silica, and to a protein-binding material prepared by coating the surface of the magnetic mesoporous silica with a transition metal or its ion so as to be capable of binding to a specific protein labeled with histidine, and also to a method of selectively separating and purifying a specific protein using the protein-binding material.
    Type: Grant
    Filed: August 11, 2011
    Date of Patent: September 16, 2014
    Assignee: Korea Institute of Ceramic Engineering and Technology
    Inventors: Jeong Ho Chang, Jiho Lee, Soo Youn Lee