Carrier-bound Or Immobilized Enzyme Or Microbial Cell; Carrier-bound Or Immobilized Cell; Preparation Thereof Patents (Class 435/174)
  • Patent number: 11253797
    Abstract: The present invention relates to a chromatographic device for isolating and purifying nucleic acids, preferably genomic DNA, by gel filtration chromatography, a method for isolating and purifying nucleic acids, preferably genomic DNA, using this device and a kit comprising this device.
    Type: Grant
    Filed: July 7, 2016
    Date of Patent: February 22, 2022
    Assignee: QIAGEN GmbH
    Inventors: Roland Fabis, Markus Müller, Jörg Hucklenbroich, Mario Scherer
  • Patent number: 11248255
    Abstract: An automated multiplex detector system includes: (a) a nucleic acid amplification compartment for amplifying nucleic acid of one or more targets in a sample, and (b) an analysis compartment in fluid communication with the amplification compartment, the analysis compartment housing a nanoparticle-based multiplex detector capable of using the amplified nucleic acid of the amplification compartment and producing a signal that correlates with the presence of the one or more targets in the sample.
    Type: Grant
    Filed: June 17, 2016
    Date of Patent: February 15, 2022
    Assignee: The Governing Council of the University of Toronto
    Inventors: Warren C. W. Chan, Jisung Kim, Kyrylo Zagorovsky
  • Patent number: 11208632
    Abstract: Antibodies that include a sulfatase motif-containing tag in a constant region of an immunoglobulin (Ig) light chain polypeptide are disclosed. The sulfatase motif can be converted by a formylglycine-generating enzyme (FGE) to produce a formylglycine (fGly)-modified Ig light chain polypeptide. An fGly-modified Ig light chain polypeptide of the antibody can be covalently and site-specifically bound to a moiety of interest to provide an antibody conjugate. The disclosure also encompasses methods of production of such tagged Ig light chain polypeptides, fGly-modified Ig light chain polypeptides, and antibody conjugates, as well as methods of use of same.
    Type: Grant
    Filed: April 24, 2017
    Date of Patent: December 28, 2021
    Assignee: R.P. Scherer Technologies, LLC
    Inventors: Yun Cheol Kim, Chao Bai Huang, David Rabuka
  • Patent number: 11167158
    Abstract: A method of custom manufacturing a flame arrestor includes providing a housing having an interior surface and an exterior surface where the exterior surface of the housing is shaped to fit within a fluid passageway. The method includes forming, using an additive manufacturing technique, a three-dimensional lattice structure by depositing a first material onto the interior surface of the housing in a predetermined pattern. The lattice structure includes a plurality of connected lattice members forming channels extending from a first end to a second end of the three-dimensional lattice structure. An element of a second material is provided adjacent to the three-dimensional lattice structure. The second material is different than the first material and the element is configured to draw heat away from fluid flowing through the plurality of channels.
    Type: Grant
    Filed: May 14, 2018
    Date of Patent: November 9, 2021
    Assignee: Emerson Process Management Regulator Technologies Tulsa, LLC
    Inventors: John D. Cockerham, James Myers
  • Patent number: 11099147
    Abstract: Disclosed are a gas sensor member, a gas sensor using the same, and manufacturing methods thereof, and specifically, a gas sensor member using a one-dimensional porous metal oxide nanotube composite material having a double average pore distribution in which mesopores (0.1 nm to 50 nm) and macropores (50 nm to 300 nm) are simultaneously formed on the surface of a nanotube through decomposition of a spherical polymer sacrificial template and continuous crystallization and diffusion of a metal oxide and a nanoparticle catalyst embedded in an apoferritin is uniformly loaded in the inside and on the outer wall and inner wall of a one-dimensional metal oxide nanotube through a high-temperature heat treatment, a gas sensor using the same, and manufacturing methods thereof are disclosed.
    Type: Grant
    Filed: March 13, 2019
    Date of Patent: August 24, 2021
    Assignee: Korea Advanced Institute of Science and Technology
    Inventors: Il-Doo Kim, Ji Su Jang, Sang-Joon Kim, Seon-Jin Choi
  • Patent number: 11046753
    Abstract: A method for conjugating an isolated antibody to a label or derivatisation reagent, which method comprises contacting the antibody with an activated label or activated derivatisation reagent, or contacting the antibody with the label or derivatisation reagent in the presence of an antibody conjugation reagent, in a buffer system which comprises a monocarboxylic acid buffer compound other than glycine, bearing an amine substituent at the alpha or beta position.
    Type: Grant
    Filed: May 30, 2014
    Date of Patent: June 29, 2021
    Inventors: Nicolas Steven Gee, Bianca Fusetti
  • Patent number: 10996181
    Abstract: Provided is a sensor comprising a non-conductive substrate; and a conductive layer electronically printed on one side of the substrate, wherein the conductive layer comprises: an antenna pattern for transmitting and receiving a radio signal with an external device; a sensing electrode connected to the antenna pattern via a circular wiring for sensing an impedance change due to contact with a sensing target material; and a coating electrode stacked on the sensing electrode for removing an occurrence of noise of the impedance change. Accordingly, the present invention solves the problem of a sensor, in the form of a terminal, not being compact and the problem of high manufacturing costs and low manufacturing quality of a sensor manufactured using a deposition method in order to replace such sensor with a sensor manufactured by a printing method, and solves a corrosion problem of a sensing electrode, a durability problem, etc. that may occur in the sensor of the printing method.
    Type: Grant
    Filed: December 20, 2017
    Date of Patent: May 4, 2021
    Inventors: Hyunchul Kim, Myungwon Lee
  • Patent number: 10995911
    Abstract: A pressure vessel for storing hydrogen is described. The pressure vessel includes at least one chamber to store hydrogen atoms. The pressure vessel also includes a mycelium structure within the at least one chamber. The mycelium structure has a surface area of at least 800 m2/m3. At least some of the hydrogen atoms are attached to the mycelium structure at a pressure greater than ambient pressure. Methods of storing hydrogen and methods of constructing a hydrogen storage tank are also described.
    Type: Grant
    Filed: February 28, 2017
    Date of Patent: May 4, 2021
    Assignee: The Boeing Company
    Inventors: Benjamin J. Stephenson, Jeffrey M. Hansen
  • Patent number: 10927419
    Abstract: The disclosure provides for methods, compositions, and kits for multiplex nucleic acid analysis of single cells. The methods, compositions and systems may be used for massively parallel single cell sequencing. The methods, compositions and systems may be used to analyze thousands of cells concurrently. The thousands of cells may comprise a mixed population of cells (e.g., cells of different types or subtypes, different sizes).
    Type: Grant
    Filed: May 23, 2018
    Date of Patent: February 23, 2021
    Assignee: Becton, Dickinson and Company
    Inventors: Christina Fan, Stephen P. A. Fodor, Glenn Fu, Geoffrey Richard Facer, Julie Wilhelmy
  • Patent number: 10927335
    Abstract: A microfluidic device includes a base comprising a chamber configured to receive a microfluidic component. A sealing member includes a body, an inlet reservoir, and an outlet reservoir, where the inlet reservoir and the outlet reservoir communicate with the chamber through fluid passages when the sealing member and base are removably coupled. A microfluidic component removably within the chamber includes microfluidic channels on a surface thereof and a tissue culture chamber coupled to at least one of the microfluidic channels. The microfluidic channels and the tissue culture chamber are in fluid communication with the inlet and outlet reservoirs through the fluid passages to form a fluid circuit for directing fluid from the inlet reservoir, through the tissue culture chamber, to the outlet reservoir, and from the outlet reservoir back to the inlet reservoir upon tilting the microfluidic device to a forward tilted position and to a reverse tilted position, respectively.
    Type: Grant
    Filed: October 3, 2016
    Date of Patent: February 23, 2021
    Assignee: Cornell University
    Inventor: Mandy Brigitte Esch
  • Patent number: 10875898
    Abstract: The present invention according to a first aspect relates to a Trichoderma longibrachiatum strain comprising an autologous nucleotide sequence coding for a protein having at least 70% sequence similarity with the amino acid sequence of SEQ ID NO: 1 and its use for the treatment of a plant. Further aspects of the invention relate to a protein derived from said strain, methods for producing the protein, the use of the protein for the treatment of a plant and compositions comprising a source of the protein of the invention. Yet Further aspects of the invention relate to seeds treated with a source of the protein and methods for growing plants incorporating the step of treating said plants with the composition of the invention.
    Type: Grant
    Filed: March 8, 2019
    Date of Patent: December 29, 2020
    Assignee: Koppert B.V.
    Inventors: Matteo Lorito, Michelina Ruocco, Francesco Vinale, Sheridan Lois Woo
  • Patent number: 10851333
    Abstract: A method, apparatus, and system are provided for the printing and maturation of living tissue in an Earth-referenced reduced gravity environment such as that found on a spacecraft or on other celestial bodies. The printing may be three-dimensional structures. The printed structures may be manufactured from low viscosity biomaterials.
    Type: Grant
    Filed: January 29, 2018
    Date of Patent: December 1, 2020
    Assignee: Techshot, Inc.
    Inventors: John C. Vellinger, Eugene Boland, Michael A. Kurk, Krystal Milliner, Nester Samuel Logan
  • Patent number: 10844425
    Abstract: Compositions, methods, kits, and uses are provided for detecting or quantifying an Human Parainfluenza virus 1 (HPIV-1), HPIV-2, HPIV-3, and/or HPIV-4 nucleic acid, e.g., using nucleic acid amplification and hybridization assays. In some embodiments, the compositions, methods, kits, and uses target the HN gene of HPIV-1, HPIV-2, and/or HPIV-3 and/or the NP gene of HPIV-4.
    Type: Grant
    Filed: March 23, 2018
    Date of Patent: November 24, 2020
    Assignee: Gen-Probe Incorporated
    Inventors: Mehrdad Majlessi, Pamela Douglass, Daniel Kolk
  • Patent number: 10828399
    Abstract: The present disclosure is directed to methods of printing 3-D structures using supramolecular gels, and the structures that result therefrom.
    Type: Grant
    Filed: March 24, 2016
    Date of Patent: November 10, 2020
    Assignee: The Trustees of the University of Pennsylvania
    Inventors: Jason Alan Burdick, Chris Highley
  • Patent number: 10815139
    Abstract: A material for reducing the content of polluting and/or unwanted substances in a liquid includes at least one polymeric and/or inorganic base, suitable to be immersed in the liquid, having at least a surface, whereto is bonded at least one catalyst of the degradation reaction of inorganic salts in gaseous substances. The catalyst, whose function is to reduce the energy required for the activation of said reaction and to accelerate its progress, is an enzyme preferably selected among the lyase group, the hydrolase group and the oxidoreductase group.
    Type: Grant
    Filed: September 25, 2017
    Date of Patent: October 27, 2020
    Assignee: ARCHIMEDE R&D S.R.L.
    Inventor: Lucio Panizza
  • Patent number: 10654917
    Abstract: The present invention relates to antibody molecules and peptide delivery systems for use in the treatment and management of Alzheimer's disease and related disorders. In particular, the antibody molecules preferentially bind oligomeric forms of beta-amyloid peptide, in single domain format, and the peptide delivery systems facilitate specific transport of such antibody molecules, as well as other cargo molecules, across the blood-brain barrier. The invention also relates to constructs of the antibody molecules and the delivery peptides, as well as pharmaceutical compositions comprising effective amounts of the antibody molecules, delivery peptides, and/or their constructs, including humanized versions of the antibody molecules and constructs.
    Type: Grant
    Filed: January 29, 2016
    Date of Patent: May 19, 2020
    Inventors: Sofia Volker Côrte-Real, Vera Luísa Santos Neves, Pedro Manuel Correia Canhão, Tiago Fleming Outeiro, Miguel Augusto Rico Botas Castanho, Frederico Nuno Castanheira Aires Da Silva, Soraia Rafaela Santiago De Oliveira
  • Patent number: 10626391
    Abstract: The invention provides granules comprising A) at least one enzyme selected from at least one of the groups selected from transferases of EC 2, hydrolases of EC 3, lyases of EC 4 and isomerases of EC 5, B) at least one polymer selected from C1-C10-alkyl acrylate polymer, C1-C10-alkyl methacrylate polymer and C1-C10-alkyl acrylate-C1-C10-alkyl methacrylate copolymer, preferably C1-C10-alkyl acrylate-C1-C10-alkyl methacrylate copolymer and C) at least one inorganic carrier material.
    Type: Grant
    Filed: March 3, 2015
    Date of Patent: April 21, 2020
    Assignee: Evonik Operations GmbH
    Inventors: Frank Hellmers, Thomas Hueller, Thomas Dassinger, Oliver Thum
  • Patent number: 10538443
    Abstract: The present invention relates to a method for degrading and inactivating at least one xenobiotic, the at least one xenobiotic being present in an aqueous medium. The method comprises the steps of (a) grafting at least one enzyme onto a solid support, (b) incubating the solid support with the at least one enzyme into the aqueous medium and (c) measuring the evolution of the concentration of the at least one xenobiotic. The method is remarkable in that the at least one enzyme is New-Dehli metallo-?-lactamase 1, a laccase extracted from Pleurotus ostreatus and/or a ?-lactamase extracted from Pseudomonas aeruginosa and in that the solid support is a moving bed carrier.
    Type: Grant
    Filed: December 14, 2015
    Date of Patent: January 21, 2020
    Inventors: Sebastien Bonot, Henry-Michel Cauchie
  • Patent number: 10525163
    Abstract: This invention relates to methods of producing collagen biomaterials by admixing (i) a solution of monomeric collagen, (ii) a solution of polymeric collagen, (ii) cargo particles, and (iv) a non-collagen blocking polymer, to produce a collagen solution. The collagen solution is then allowed to solidify to produce a collagen hydrogel. This may be useful in improving the stiffness of collagen hydrogel constructs and increasing the entrapment and retention of cargo particles.
    Type: Grant
    Filed: June 5, 2015
    Date of Patent: January 7, 2020
    Inventor: Josephine Wong
  • Patent number: 10512696
    Abstract: The invention is directed to biocompatible conjugated polymer nanoparticles including a copolymer backbone, a plurality of sidechains covalently linked to said backbone, and a plurality of platinum compounds dissociably linked to said backbone. The invention is also directed to dicarbonyl-lipid compounds wherein a platinum compound is dissociably linked to the dicarbonyl compound. The invention is also directed to methods of treating cancer or metastasis. The methods includes selecting a subject in need of treatment for cancer or metastasis and administering to the subject an effective amount of any of the nanoparticles, compounds, or compositions of the invention.
    Type: Grant
    Filed: June 15, 2016
    Date of Patent: December 24, 2019
    Inventors: Shiladitya Sengupta, Abhimanyu Paraskar, Shivani Soni, Sudipta Basu, Poulomi Sengupta
  • Patent number: 10508297
    Abstract: The present invention provides an insoluble enzymatic reagent for detecting products derived from glucuronide metabolites in a sample, containing an enzyme with glucuronidase activity immobilized on a resin comprising a polysaccharide modified with chemical groups, and optionally a cationic polymer. Additionally, it provides methods for preparing said enzymatic reagent and for detecting derivatives of glucuronide metabolites in a sample, using said reagent.
    Type: Grant
    Filed: July 13, 2017
    Date of Patent: December 17, 2019
    Inventors: Manuel Rozas Andreu, Fernando Alexis Gutierrez Rojas, Rocio Andrea Peralta Villalobos
  • Patent number: 10479971
    Abstract: A culture container has a first inflow port and a first outflow port. The first flow path connects the first outflow port to the first inflow port. A storage container is provided within the first flow path and has a second inflow port which is connected to the first outflow port and a second outflow port which is connected to the first inflow port. A second flow path connects a first region within the first flow path, and a second region within the first flow path. A division processing portion is provided within the second flow path, performs a division process of dividing a cell aggregation flowing in from the first flow path, and allows the cells subjected to the division process to flow out into the first flow path via the second region. A medium supply portion supplies a medium to the inside of the first flow path.
    Type: Grant
    Filed: July 14, 2017
    Date of Patent: November 19, 2019
    Inventors: Hideaki Kagawa, Shun Goto, Souichi Kohashi, Hidekazu Yamazaki
  • Patent number: 10441610
    Abstract: Disclosed are methods and compositions of microbead carriers for delivery of cells and other biologically active substances to diseased or damaged tissue in a subject in need thereof.
    Type: Grant
    Filed: November 23, 2011
    Date of Patent: October 15, 2019
    Assignee: Georgia Tech Research Corporation
    Inventors: Barbara Dale Boyan, Zvi Schwartz, Christopher S. D. Lee, Shirae Kerisha Leslie, Ramsey C. Kinney
  • Patent number: 10436682
    Abstract: A Raman silent substrate includes a base and a biologically compatible material layer over the base. The biologically compatible material layer is Raman silent, provides a predetermined capture efficiency corresponding to a desired application, and supports biological culturing. In certain implementations, the base comprises a glass layer and an aluminum layer between the glass layer and the biologically compatible material layer.
    Type: Grant
    Filed: May 29, 2016
    Date of Patent: October 8, 2019
    Inventors: Andrew P. Bartko, Theodore J. Ronningen
  • Patent number: 10435676
    Abstract: The present invention relates to a variant of Terminal deoxynucleotidyl Transferase (TdT) which (i) comprises the amino acid sequence as set forth in SEQ ID Nº 2 or a functionally equivalent sequence, with at least an amino acid substitution at position corresponding to residue C302 or functionally equivalent residue, wherein the position is numbered by reference to the amino acid sequence set forth in SEQ ID Nº 1, (ii) is able to synthesize a nucleic acid fragment without template and (iii) is able to incorporate a modified nucleotide into the nucleic fragment.
    Type: Grant
    Filed: January 8, 2019
    Date of Patent: October 8, 2019
    Inventors: Elise Champion, Mikhael Soskine, Thomas Ybert, Marc Delarue
  • Patent number: 10429665
    Abstract: A synthetic aperture optics (SAO) imaging method minimizes the number of selective excitation patterns used to illuminate the imaging target, based on the objects' physical characteristics corresponding to spatial frequency content from the illuminated target and/or one or more parameters of the optical imaging system used for SAO. With the minimized number of selective excitation patterns, the time required to perform SAO is reduced dramatically, thereby allowing SAO to be used with DNA sequencing applications that require massive parallelization for cost reduction and high throughput. In addition, an SAO apparatus optimized to perform the SAO method is provided. The SAO apparatus includes a plurality of interference pattern generation modules that can be arranged in a half-ring shape.
    Type: Grant
    Filed: August 24, 2017
    Date of Patent: October 1, 2019
    Assignee: Optical Biosystems, Inc.
    Inventors: Chun-Sheu Lee, Jong Buhm Park
  • Patent number: 10400275
    Abstract: The present invention relates to in situ hybridization methods for detecting a target nucleic acid in a biological sample comprising performing one or more method steps (e.g., pretreatment, denaturation, hybridization, washes) at room temperature. The invention further relates to kits for performing such methods.
    Type: Grant
    Filed: September 3, 2015
    Date of Patent: September 3, 2019
    Assignee: Cellay, Inc.
    Inventor: Joan Aurich-Costa
  • Patent number: 10281473
    Abstract: The application discloses compositions, methods, systems, and apparatuses for rapid sequence analysis of proteins, including location of post-translational modifications and disulfide bonds. Limited digestion of fully denatured antibody occurs in seconds by flowing sample in 8M urea at constant pressure through a micro column reactor containing immobilized aspergillopepsin I, resulting in a product mixture containing 3-10 kDa peptides, which is then fractionated by capillary column chromatography and analyzed by both electron transfer dissociation (ETD) and collision activated dissociation mass spectrometry. This method provides 95% sequence coverage of a mAb and detects numerous post-translational modifications. For disulfide bond location, native mAb is subjected to longer digestion times. Release of disulfide containing peptides from accessible regions of the folded protein occurs with short digestion times.
    Type: Grant
    Filed: February 4, 2015
    Date of Patent: May 7, 2019
    Assignee: University of Virginia Patent Foundation
    Inventors: Donald F. Hunt, Weihan Wang, Lichao Zhang
  • Patent number: 10221354
    Abstract: A luminescent particle including a surface comprising glass that surrounds one or more particles of one or more light emissive materials is disclosed. Preferably the surface comprises a vitrified glass. Methods form making a luminescent particle including a surface comprising glass that surrounds one or more particles of one or more light emissive materials is also disclosed. Compositions and products including a luminescent particle are further disclosed.
    Type: Grant
    Filed: May 19, 2016
    Date of Patent: March 5, 2019
    Inventor: Robert J. Nick
  • Patent number: 10202587
    Abstract: Provided is a mutated hyperthermophilic PTE having a lactonase activity derived from a hyperthermophilic phosphotriesterase corresponding to the consensus sequence of SEQ ID NO: 1, the mutated PTE including the at least one mutation chosen amongst 55 putative positions and the mutated PTE having enhanced properties. Also provided are compositions including the mutated hyperthermophilic PTE and the uses thereof, notably as bioscavenger of organophosphate compounds or as quorum quencher of the bacteria using lactones to communicate.
    Type: Grant
    Filed: July 31, 2014
    Date of Patent: February 12, 2019
    Inventors: Eric Chabriere, Mikael Elias, Julien Hiblot, Didier Raoult
  • Patent number: 10174353
    Abstract: The present invention relates to a method of preparing at least one rhamnolipid comprising: contacting a recombinant cell with a medium containing a carbon source; and culturing the cell under suitable conditions for preparation of the rhamnolipid from the carbon source by the cell, wherein the recombinant cell has been genetically modified such that, compared to the wild-type of the cell, the cell has an increased activity of at least one of the enzymes E1, E2 and E3, wherein the enzyme E1 is an ?/? hydrolase, the enzyme E2 is a rhamnosyltransferase I and the enzyme E3 is a rhamnosyl-transferase II, and wherein the carbon source is a C4 molecule.
    Type: Grant
    Filed: April 27, 2015
    Date of Patent: January 8, 2019
    Assignee: Evonik Degussa GmbH
    Inventors: Oliver Thum, Philip Engel, Christian Gehring, Steffen Schaffer, Mirja Wessel
  • Patent number: 10119973
    Abstract: The present invention relates to multiplex microarrays and methods for the quantification of analytes. In particular, the invention relates to improved methods which standardize a target analyte concentration in a test sample against a reference standardization curve derived from validated, approved and recognized reference standards for the target analyte of known concentrations. The present invention also relates to methods and checks for simultaneous measurement of confidence confirming normalization standards and controls.
    Type: Grant
    Filed: October 29, 2010
    Date of Patent: November 6, 2018
    Inventors: Peter Lea, Jennifer Hansen, Kate Smith
  • Patent number: 10101247
    Abstract: This disclosure is directed to a method and an attachment solution for adhering a cytological or histological sample, such as buffy coat, to a substrate, such as a microscope slide. The attachment solution includes an attachment base, an anti-coagulant, and a nonsteroidal anti-inflammatory drug. The attachment base may be an alcohol, an acid, an oxidizer, an organohalogen, a ketone, or any combination thereof. Once a sample is obtained, the sample may be re-suspended in the attachment solution or the attachment solution may be added to the sample. The sample may then be dispensed onto an analysis platform as one or more droplets and cured.
    Type: Grant
    Filed: December 2, 2015
    Date of Patent: October 16, 2018
    Assignee: RareCyte, Inc.
    Inventors: Daniel Campton, Joshua Nordberg, Ronald Seubert, Steve Quarre
  • Patent number: 10010074
    Abstract: Embodiments of the present disclosure, in one aspect, relate to structures having a polymer composition covalently bonded to the surface of the structure, methods of attaching the polymer to the surface of the structure, methods of decreasing the amount of microorganisms formed on a structure, materials, methods of attaching materials, and the like.
    Type: Grant
    Filed: August 8, 2017
    Date of Patent: July 3, 2018
    Inventor: Jason J. Locklin
  • Patent number: 9920380
    Abstract: The invention provides a diverse population of uniquely labeled probes, containing about thirty or more target specific nucleic acid probes each attached to a unique label bound to a nucleic acid. Also provided is a method of producing a population of uniquely labeled nucleic acid probes. Also provided is a method of detecting a nucleic acid analyte. The method consists of (a) contacting a mixture of nucleic acid analytes under conditions sufficient for hybridization with a plurality of target specific nucleic acid probes each having a different specifier; (b) contacting the mixture under conditions sufficient for hybridization with a corresponding plurality of anti-genedigits each having a unique label, the plurality of anti-genedigits having a diversity sufficient to uniquely hybridize to genedigits within the specifiers, and (c) uniquely detecting a hybridized complex between one or more analytes in the mixture, a target specific probe, and an anti-genedigit.
    Type: Grant
    Filed: March 11, 2013
    Date of Patent: March 20, 2018
    Assignee: The Institute for Systems Biology
    Inventors: Krassen M. Dimitrov, Dwayne L. Dunaway
  • Patent number: 9879117
    Abstract: Structures having a polymer composition covalently bonded to the surface of the structure, methods of attaching the polymer to the surface of the structure, methods of decreasing the amount of microorganisms formed on a structure, and the like, are disclosed.
    Type: Grant
    Filed: January 27, 2017
    Date of Patent: January 30, 2018
    Assignee: University of Georgia Research Foundation, Inc.
    Inventors: Jason J. Locklin, Vikram Dhende
  • Patent number: 9878323
    Abstract: We describe assay modules (e.g., assay plates, cartridges, multi-well assay plates, reaction vessels, etc.), processes for their preparation, and method of their use for conducting assays. Reagents may be present in free form or supported on solid phases including the surfaces of compartments (e.g., chambers, channels, flow cells, wells, etc.) in the assay modules or the surface of colloids, beads, or other particulate supports. In particular, dry reagents can be incorporated into the compartments of these assay modules and reconstituted prior to their use in accordance with the assay methods. A desiccant material may be used to maintain and stabilize these reagents in a dry state.
    Type: Grant
    Filed: October 29, 2012
    Date of Patent: January 30, 2018
    Inventors: Eli N. Glezer, Bandele Jeffrey-Coker, Jeffery D. Debad, Sudeep M. Kumar, George B. Sigal, Gisbert Spieles, Michael Tsionsky, Michael Warnock
  • Patent number: 9828576
    Abstract: The disclosure relates to the fabrication of a three dimensional [3-D] cell culture membrane comprising one or more functionalized surfaces adapted to provide cell culture conditions suitable for the analysis of proliferation, differentiation or function of cells, typically eukaryotic or prokaryotic cells.
    Type: Grant
    Filed: July 29, 2013
    Date of Patent: November 28, 2017
    Assignees: National University of Singapore, Centre National de la Recherche Scientifique
    Inventors: Virgile Nicolas Robert Viasnoff, Qiushi Li
  • Patent number: 9810692
    Abstract: The present invention relates to a method of assessing, predicting or monitoring the sensitivity of a subject having a tumor or cancer to an immunotherapeutic molecule acting on the subject's T cells, to a method of selecting an appropriate treatment of cancer, to a method of screening or identifying a compound suitable for improving the treatment of a cancer, and to the use of corresponding kits. The method of predicting or monitoring the sensitivity of a subject having a tumor to an immunotherapeutic molecule acting on the subject's T cells typically comprises a step a) of determining, in a biological sample from said subject, the expression level of soluble CD25 (sCD25) and, when the expression level is determined, a step b) of comparing at least said expression level to a reference expression level, thereby assessing whether the subject having a tumor is responsive or resistant to the immunotherapeutic molecule.
    Type: Grant
    Filed: October 25, 2013
    Date of Patent: November 7, 2017
    Inventors: Dalil Hannani, Laurence Zitvogel, Caroline Robert, Nathalie Chaput-Gras
  • Patent number: 9789439
    Abstract: The present invention relates to the use of solid metal materials for catalyzing the hydration of carbon dioxide. It also relates to methods of and apparatus for hydrating carbon dioxide and capturing carbon. The solid metal materials may be nickel nanoparticles. The invention finds particular application in the sequestration of carbon dioxide either at the point of release or from the atmosphere.
    Type: Grant
    Filed: May 14, 2013
    Date of Patent: October 17, 2017
    Assignee: University of Newcastle upon Tyne
    Inventors: Lidija Siller, Gaurav Ashok Bhaduri
  • Patent number: 9758558
    Abstract: A biodegradable hydrogel has been made based on high concentrations of whey protein isolate (WPI). WPI gels of different compositions were fabricated by thermally inducing gelation of high-concentration suspensions of protein, and characterized for compressive strength and modulus, hydration swelling and drying properties, mechanical behavior change due to polysaccharide additives, and intrinsic pore network structure. The gels were shown to be compatible with bone cells and could be used as bone tissue scaffolds. In addition, WPI fibers were produced by electrospinning. Several additives could be incorporated into the WPI gels, including structural additives, growth factors, amino acids, etc. The WPI hydrogels can be made with glycerol to increase flexibility and stability.
    Type: Grant
    Filed: April 1, 2011
    Date of Patent: September 12, 2017
    Assignee: Board of Supervisors of Louisiana State University And Agriculture and Mechanical College
    Inventors: James E. Henry, Mia Dvora
  • Patent number: 9724417
    Abstract: Compositions are provided in which dendrimers and/or nanoparticles are synthesized with multi-photon responsive elements and self-immolative oligomers. The compositions may be utilized to selectively deliver Payloads within tissue by irradiating the compositions. The compositions may also be used to amplify sensitivity to irradiation.
    Type: Grant
    Filed: August 8, 2014
    Date of Patent: August 8, 2017
    Inventors: Adah Almutairi, Nadezda Fomina, Jagadis Sankaranarayanan
  • Patent number: 9709479
    Abstract: A method of tracking cell identity across analytical platforms uses stochastic barcoding (SB). SB uses a randomly generated code based on one or more of the number, color and position of beads encapsulated together with a set of cells of interest. SB use is demonstrated in an application where cells are transferred from a microwell array into a microtiter plate while keeping their identity, and obtained an average identification accuracy of 96% for transfer of 100 blocks. Model scaling of the method up to 1000 blocks demonstrated that SB is able to achieve approximately 90% accuracy.
    Type: Grant
    Filed: October 24, 2014
    Date of Patent: July 18, 2017
    Inventors: Marc Castellarnau, Gregory Lee Szeto, Darrell J. Irvine, John Christopher Love, Joel Voldman
  • Patent number: 9694338
    Abstract: Hydrogel arrays, methods for preparing hydrogel arrays and methods for screening cell-substrate interactions using the hydrogel arrays are disclosed. Advantageously, the hydrogel arrays include individual hydrogel posts that are completely isolatable, allowing for systematic and independent control of the chemical composition and physical dimensions of each hydrogel post.
    Type: Grant
    Filed: December 18, 2014
    Date of Patent: July 4, 2017
    Assignee: Wisconsin Alumni Research Foundation
    Inventors: William Murphy, Ngoc Nhi Le
  • Patent number: 9671407
    Abstract: The present invention provides a device for isolating target biomolecules or cells from samples, particularly biological samples. In particular, the device comprises a loading mixture, which contains the biological sample and a first binding entity that specifically binds to the target biomolecule or target cell; and a micro-channel coated with a second binding entity that binds directly or indirectly to the first binding entity. Methods of capturing, detecting, and/or evaluating target biomolecules or target cells (e.g. cancer cells) in biological samples are also disclosed.
    Type: Grant
    Filed: March 24, 2014
    Date of Patent: June 6, 2017
    Assignee: BIOCEPT, INC.
    Inventors: Stephen D. Mikolajczyk, Tony Pircher, Farideh Z. Bischoff, Pavel Tsinberg
  • Patent number: 9658220
    Abstract: The present disclosure provides compositions and methods that are useful for normalizing the amount of signal detected in an assay, such as an immunoassay. The compositions and methods are useful for improving the accuracy of immunoassays, such as immunoassays that detect whether a subject is infected with a retrovirus such as HIV.
    Type: Grant
    Filed: June 20, 2013
    Date of Patent: May 23, 2017
    Assignee: Bio-Rad Laboratories, Inc.
    Inventors: David King, William F. Link, Renato del Rosario, Michael Leos
  • Patent number: 9618840
    Abstract: Polymer Pen Lithography materials that retain the simplicity characteristic for the fabrication of poly(dimethyl siloxane) tip arrays while preserving the control over feature dimensions and to understand the role of the mechanical properties of the different materials on Polymer Pen Lithography printing.
    Type: Grant
    Filed: November 19, 2013
    Date of Patent: April 11, 2017
    Inventors: Adam B. Braunschweig, Xiao Zhong, Kevin B. Schesing, Shudan Bian
  • Patent number: 9615584
    Abstract: Described herein are eco-friendly formulations and methods for providing such formulations to plants. The formulations include mixtures of microbial isolates. In particular, numerous bacterial and fungal strains were isolated from a variety of soil types, from rhizospheres and from root nodules of leguminous plants, in designed combinations, for providing plant growth and plant productivity enhancing formulations. These specifically designed polymicrobial formulations provide protection against plant pathogens, lower the need for nitrogen containing fertilizers, solubilize minerals, protect plants against pathogens, and make available to the plant valuable nutrients, such as phosphate, thus reducing and eliminating the need for using chemical fertilizers and chemical pesticides.
    Type: Grant
    Filed: January 22, 2014
    Date of Patent: April 11, 2017
    Assignee: Board of Trustees of Michigan State University
    Inventors: C. Adinarayana Reddy, Lalithakumari Janarthanam
  • Patent number: 9603798
    Abstract: An antibody-conjugated double-emulsion nanocapsule is provided. A linking group is introduced on the surface of a double-emulsion nanocapsule, which is composed of an oily shell enclosing an aqueous core, to link the double-emulsion nanocapsule with an antibody.
    Type: Grant
    Filed: April 24, 2014
    Date of Patent: March 28, 2017
    Inventors: Chih-Sheng Chiang, Shang-Hsiu Hu, San-Yuan Chen
  • Patent number: 9601321
    Abstract: A measurement state in a mass spectrometer device is determined so that the measurement method for the next round of measurement can be automatically determined. The mass spectrometer device (1) is provided with: a first calculation unit (6) that calculates the total amount of ion in a mass spectrum; a second calculation unit (6) that calculates the half-value width of a representative peak selected from peaks appearing in the mass spectrum; and a control unit (7) that determines the measurement method for use in the next round of measurement on the basis of the total amount of ion and the half-value width of the representative peak.
    Type: Grant
    Filed: June 14, 2013
    Date of Patent: March 21, 2017
    Assignee: Hitachi High-Technologies Corporation
    Inventors: Akihito Kaneko, Yohei Kawaguchi, Masuyuki Sugiyama, Kazushige Nishimura