Transferring Phosphorus Containing Group (e.g., Kineases, Etc.(2.7)) Patents (Class 435/194)
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Patent number: 12221634Abstract: The present disclosure provides methods and systems for amplifying and analyzing nucleic acid samples. The present disclosure provides methods for preparing cDNA and/or DNA molecules and cDNA and/or DNA libraries using modified reverse transcriptases.Type: GrantFiled: August 14, 2023Date of Patent: February 11, 2025Assignee: BIO-RAD LABORATORIES, INC.Inventors: Arkadiusz Bibillo, Pranav Patel
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Patent number: 12221633Abstract: The present disclosure provides methods and systems for amplifying and analyzing nucleic acid samples. The present disclosure provides methods for preparing cDNA and/or DNA molecules and cDNA and/or DNA libraries using modified reverse transcriptases.Type: GrantFiled: July 7, 2023Date of Patent: February 11, 2025Assignee: BIO-RAD LABORATORIES, INC.Inventors: Arkadiusz Bibillo, Pranav Patel
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Generating nucleic acids with modified bases using recombinant terminal deoxynucleotidyl transferase
Patent number: 12209266Abstract: Disclosed herein include methods of generating a single stranded deoxyribonucleic acid (ssDNA) scaffold comprising nucleotides with modified bases using a recombinant terminal deoxynucleotidyl transferase (TdT). The recombinant TdT can comprise an amino acid sequence that is at least 80% identical to a Bos taurus TdT, or a fragment thereof, and for example comprise one or more amino acid substitution mutations at one or more positions functionally equivalent to Glu191, Lys193, Glu194, Asp242, Lys287, Phe296, Met299, Thr342, and His420 in the Bos taurus TdT.Type: GrantFiled: May 11, 2021Date of Patent: January 28, 2025Assignees: Illumina, Inc., Illumina Singapore Pte. Ltd.Inventors: Saurabh Rajendra Nirantar, Sergio Peisajovich, Eric Brustad -
Patent number: 12195731Abstract: The disclosure provides methods and reagents for preparing DNA libraries from biological materials for targeted sequencing. The approach can enhance the efficiency and sensitivity of targeted sequencing applications, such as liquid biopsy analyses to assess genetically driven conditions.Type: GrantFiled: July 23, 2019Date of Patent: January 14, 2025Assignee: Salish Bioscience Inc.Inventor: Christopher K. Raymond
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Patent number: 12169198Abstract: The present disclosure provides systems and methods for processing or analyzing a sample. Methods may include generating supports (e.g., beads) comprising barcode molecules coupled thereto. A support comprising barcode molecules may be useful for analyzing or processing one or more analytes such as nucleic acid molecules, proteins, and/or perturbation agents.Type: GrantFiled: August 18, 2023Date of Patent: December 17, 2024Assignee: 10X GENOMICS, INC.Inventors: Andrew D. Price, Christopher Hindson
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Patent number: 12168046Abstract: The present disclosure relates, at least in part, to mycobacterial polynucleotides and polypeptides, to fragments or variants thereof, to cells comprising the mycobacterial polynucleotides and polypeptides, to cells comprising the mycobacterial polynucleotides and polypeptides, that are engineered to expand T-cells ex vivo, and to methods of use thereof.Type: GrantFiled: April 26, 2019Date of Patent: December 17, 2024Assignee: Children's National Medical CenterInventors: Michael Keller, Catherine Bollard, Patrick Hanley, Conrad Russell Cruz, Haili Lang, Shabnum Patel
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Patent number: 12077789Abstract: Presented herein are altered polymerase enzymes for improved incorporation of nucleotides and nucleotide analogues, in particular altered polymerases that maintain low pre-phasing rates when using ambiently stored polymerases, as well as methods and kits using the same.Type: GrantFiled: June 1, 2022Date of Patent: September 3, 2024Assignee: ILLUMINA, INC.Inventors: Misha Golynskiy, Mariko Matsuura, Saurabh Nirantar, Hamed Tabatabaei Ghomi, Seth McDonald, Ryan Craig, Sergio Peisajovich, Kyrie Johnson, Kay Klausing, Eric Murtfeldt, Alexandra Exner
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Patent number: 11821032Abstract: The present invention provides methods, compositions, mixtures and kits utilizing 5-Chloro-2-methyl-4-isothiazolin-3-one in sequencing reactions, and in particular, sequencing reactions where deoxynucleoside triphosphates comprising a 3?-O position capped by a disulfide-based 3?-terminator group are used. In one embodiment, the deoxynucleoside triphosphates comprise a 3?-O position capped by a group comprising methylenedisulfide as a cleavable protecting group and a detectable label reversibly connected to the nucleobase of said deoxynucleoside. In addition, thiol-containing compounds and scavengers of thio-containing compounds are described. Such compounds provide new possibilities for future sequencing technologies, including but not limited to Sequencing by Synthesis.Type: GrantFiled: August 21, 2020Date of Patent: November 21, 2023Assignee: IsoPlexis CorporationInventors: Luisa Andruzzi, Jimmy Adediran, Timothy Pelletier, Austin Ricker, Angela DeLucia, Ben McNally, Dona Hevroni, Mong Sano Marma, John Andrew Sheridan
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Patent number: 11795452Abstract: Compositions and methods are provided herein for conducting prime editing of a target DNA molecule (e.g., a genome) that enables the incorporation of a nucleotide change and/or targeted mutagenesis. The compositions include fusion proteins comprising nucleic acid programmable DNA binding proteins (napDNAbp) and a polymerase (e.g., reverse transcriptase), which is guided to a specific DNA sequence by a modified guide RNA, named a PEgRNA. The PEgRNA has been altered (relative to a standard guide RNA) to comprise an extended portion that provides a DNA synthesis template sequence which encodes a single strand DNA flap which is synthesized by the polymerase of the fusion protein and which becomes incorporated into the target DNA molecule.Type: GrantFiled: May 23, 2022Date of Patent: October 24, 2023Assignees: The Broad Institute, Inc., President and Fellows of Harvard CollegeInventors: David R. Liu, Andrew Vito Anzalone
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Patent number: 11667911Abstract: The present disclosure is directed to methods of producing a modified nucleic acid comprising a precise deletion in a target nucleic acid in a cell comprising generating, within the cell, a first single strand break on a first strand of the target nucleic acid and a second single strand break on a second strand of the target nucleic acid, thereby forming a double strand break in the target nucleic acid having a first 3? overhang and a second 3? overhang; processing the first 3? overhang and the second 3? overhang with an exonuclease molecule, thereby deleting the segment of the target nucleic acid that was located between the first single strand break and the second single strand break, and forming a processed double strand break; and allowing the processed double strand break to be repaired by at least one DNA repair pathway, thereby producing the modified nucleic acid comprising the precise deletion in the target nucleic acid in the cell.Type: GrantFiled: September 23, 2016Date of Patent: June 6, 2023Assignee: Editas Medicine, Inc.Inventors: Anne Helen Bothmer, Cecilia Cotta-Ramusino, Luis A. Barrera
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Patent number: 11618891Abstract: This disclosure relates to thermophilic family B DNA polymerases comprising a neutral amino acid residue at a certain position near the C-terminus of the catalytic domain, which corresponds to a position occupied by a basic amino acid residue in wild-type Pfu polymerase. The thermophilic family B DNA polymerases provided herein also comprise an N-terminal domain comprising a uracil-binding pocket that has been modified to reduce template uracil binding. Related uses, methods, and compositions are also provided. In some embodiments, the polymerases comprise a 3?-5? exonuclease domain and/or a sequence non-specific dsDNA binding domain.Type: GrantFiled: June 25, 2018Date of Patent: April 4, 2023Assignee: Thermo Fisher Scientific Baltics UABInventors: Rasa Sukackaite, Skaiste Valinskyte
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Patent number: 11572388Abstract: The invention provides novel peptides (e.g., linkers) and polypeptide compositions comprising the linkers (e.g., fusion proteins) and methods of using the polypeptide compositions. Peptides (e.g., linkers) are useful as tags and for engineering fusion proteins (e.g., antigen binding molecules, scFv). Polypeptide linkers described herein facilitate flexibility of linked peptides allowing for proper folding, conformation and reduced immunogenicity.Type: GrantFiled: May 19, 2020Date of Patent: February 7, 2023Assignee: Kite Pharma, Inc.Inventors: Jed J. W. Wiltzius, Stuart A. Sievers
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Patent number: 11548909Abstract: The present invention provides methods, compositions, mixtures and kits utilizing deoxynucleoside triphosphates comprising a 3?-O position capped by a group comprising methylenedisulfide as a cleavable protecting group and a detectable label reversibly connected to the nucleobase of said deoxynucleoside. Such compounds provide new possibilities for future sequencing technologies, including but not limited to Sequencing by Synthesis.Type: GrantFiled: March 22, 2018Date of Patent: January 10, 2023Assignee: IsoPlexis CorporationInventors: Mong Sano Marma, Jerzy Olejnik, Ilia Korboukh
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Patent number: 11542524Abstract: Provided herein are methods and compositions for a suicide gene approach comprising an expression vector comprising a cell cycle-dependent promoter driving the expression of a suicide gene. Also provided herein are methods to render proliferative cells sensitive to a prodrug after transplantation but avoids expression of the suicide gene in post-mitotic cells, such as neurons.Type: GrantFiled: November 29, 2018Date of Patent: January 3, 2023Assignee: Research Development FoundationInventors: Karl-Heinz Krause, Michel Dubois-Dauphin, Vannary Tieng Caulet
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Patent number: 11512295Abstract: Provided herein are modified Archaeal family B polymerases derived from the Archaeal microorganism Thermococcus sp. EP1 that exhibit improved incorporation of nucleotide analogues utilized in DNA sequences.Type: GrantFiled: September 10, 2020Date of Patent: November 29, 2022Assignee: Singular Genomics Systems, Inc.Inventors: Souad Naji, Carl W. Fuller, Eli N. Glezer, Andrew Spaventa
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Patent number: 11505830Abstract: Provided herein are methods and compositions for multiplex detection of a large number of actionable gene fusions with very high sensitivity and specificity. The present methods and compositions can detect ALK, RET, and ROS1 gene fusions, optionally in combination with other mutations and fusions.Type: GrantFiled: May 30, 2018Date of Patent: November 22, 2022Assignee: Roche Molecular Systems, Inc.Inventors: Ann Begovich, Cindy Cheung, Javelin Chi, Grantland Hillman, Dwight Kuo, Michael Lee, Chitra Manohar, Xiaoju Max Ma, Ellen Ordinario, Jaya Rajamani, Huan Truong
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Patent number: 11499194Abstract: Compositions and methods useful for the diagnosis and treatment of juvenile idiopathic arthritis are disclosed.Type: GrantFiled: May 16, 2019Date of Patent: November 15, 2022Assignee: THE CHILDREN'S HOSPITAL OF PHILADELPHIAInventors: Terri H. Finkel, Haitao Zhang, Hakon Hakonarson
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Patent number: 11473140Abstract: The present invention relates to the field of nucleic acid sequence replication including PCR. Specifically, the present invention relates to methods and compositions for amplifying one or more target sequences from one or more template sequences. In particular, the present invention provides novel primer designs to enhance specificity of PCR reactions. The present invention also provides methods and compositions to perform the selection of specific sequence sections using specific primers and the amplification of all selected sequence sections using a pair of common primers in a single reaction tube.Type: GrantFiled: November 26, 2014Date of Patent: October 18, 2022Assignee: LC Sciences LCInventors: Xiaochuan Zhou, Qi Zhu, Nijing Sheng
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Patent number: 11421264Abstract: The present invention provides methods, compositions, mixtures and kits utilizing deoxynucleoside triphosphates comprising a 3?-O position capped by a group comprising methylenedisulfide as a cleavable protecting group and a detectable label reversibly connected to the nucleobase of said deoxynucleoside. In addition, thiol-containing compounds and scavengers of thio-containing compounds are described. Such compounds provide new possibilities for future sequencing technologies, including but not limited to Sequencing by Synthesis.Type: GrantFiled: March 13, 2017Date of Patent: August 23, 2022Assignee: IsoPlexis CorporationInventors: Mong Sano Marma, Luisa Andruzzi, Ben McNally, Angela DeLucia, Dona Hevroni, Elizabeth Carpenter, Magnus Besev
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Patent number: 11396677Abstract: This disclosure provides systems and methods for attaching nanopore-detectable tags to nucleotides. The disclosure also provides methods for sequencing nucleic acids using the disclosed tagged nucleotides.Type: GrantFiled: March 25, 2019Date of Patent: July 26, 2022Assignees: THE TRUSTEES OF COLUMBIA UNIVERSITY IN THE CITY OF NEW YORK, ROCHE SEQUENCING SOLUTIONS, INC.Inventors: Carl W. Fuller, Shiv Kumar, Jingyue Ju, Randall Davis, Roger Chen
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Diagnostic method based on large scale identification of post-translational modification of proteins
Patent number: 11333668Abstract: Methods for the large scale identification of post-translational modification states of proteins and enzyme activities for carrying out post-translational modification reactions involve the analysis of functional extracts from fresh and frozen samples using protein arrays. The methods and kits of the present invention can be used to analyze and characterize compounds for their effects on post-translational modifications and their pathways. The methods and kits can also be used to diagnose and characterize a wide variety of diseases and medical conditions, including cancer, neurodegenerative diseases, immune diseases, infectious diseases, genetic diseases, metabolic conditions, and drug effects using cells or body fluids of a patient.Type: GrantFiled: July 10, 2015Date of Patent: May 17, 2022Assignee: President and Fellows of Harvard CollegeInventors: Yifat Merbl, Marc W. Kirschner -
Patent number: 11299776Abstract: Disclosed are compositions and methods for nucleic acid amplification and detection. Specifically, disclosed herein are compositions and methods that allow for amplification of nucleic acids at a wide variety of temperatures. This includes a polymerase which is thermostable at high temperatures, and a method of amplification that can be conducted at relatively low temperatures.Type: GrantFiled: May 10, 2018Date of Patent: April 12, 2022Assignee: BOARD OF REGENTS, THE UNIVERSITY OF TEXAS SYSTEMInventors: Andrew Ellington, Cheulhee Jung, Sheng Cai, Sanchita Bhadra, John N. Milligan, Daniel Garry, Raghav Shroff
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Patent number: 11299720Abstract: Compositions that include polymerases with features for improving entry of nucleotide analogues into active site regions and for coordinating with the nucleotide analogues in the active site region are provided. Methods of making the polymerases and of using the polymerases in sequencing and DNA replication and amplification as well as kinetic models of polymerase activity and computer-implemented methods of using the models are also provided.Type: GrantFiled: July 14, 2020Date of Patent: April 12, 2022Assignee: Pacific Biosciences of California, Inc.Inventors: David K. Hanzel, Geoff Otto, Paul S. Peluso, Thang Pham, David R. Rank, Fred Christians, Arekadiusz Bibillo, Insil Park, Sonya Clark, John Lyle
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Patent number: 11273208Abstract: Disclosed herein are methods of treating an ischemic or inflammatory condition in an organ or tissue of a patient comprising inducing an increase of the level of CAMKK1 in said organ or tissue.Type: GrantFiled: May 31, 2017Date of Patent: March 15, 2022Assignees: Summa Health, Northeast Ohio Medical UniversityInventors: Marc S. Penn, Matthew Kiedrowski, Maritza Mayorga
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Patent number: 11248271Abstract: The disclosure describes a method for sequencing long portions of DNA sequence by assembling a plurality of shorter polynucleotide reads. Generally, the method includes annealing a plurality of primers to a denatured DNA molecule, appending a barcode polynucleotide to the 5? end of the primer, subjecting the DNA molecules to a plurality of cycles of (1) pooling, (2) dividing, and (3) appending a barcode polynucleotide to the 5? end of the primer, sequencing the barcode polynucleotides and the genomic DNA, and assembling the short read polynucleotide sequences having identical barcode polynucleotides.Type: GrantFiled: March 29, 2019Date of Patent: February 15, 2022Assignee: UNM Rainforest InnovationsInventor: Jeremy Scott Edwards
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Patent number: 11180740Abstract: Reaction compositions are disclosed herein comprising at least water, alpha-glucose-1-phosphate (alpha-G1P), an acceptor molecule, and a beta-1,3-glucan phosphorylase enzyme. These reactions can synthesize oligosaccharides and polysaccharides with beta-1,3 glycosidic linkages. Further disclosed are methods of isolating beta-1,3-glucan.Type: GrantFiled: April 15, 2019Date of Patent: November 23, 2021Assignee: DANISCO US INCInventors: Zheyong Yu, Slavko Kralj, Natnael Behabtu, Zhenghong Zhang, Laurie A Howe
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Patent number: 11168312Abstract: The invention includes a mutant Taq polymerase, which can effectively amplify a target sequence under conditions of salt concentration(s) similar to body fluids, including blood, serum or plasma preserved with sodium citrate. The mutant Taq polymerase, or a biologically active fragment thereof, has one or more substitutions differing from the wild type as shown in Table I.Type: GrantFiled: March 9, 2020Date of Patent: November 9, 2021Assignee: ABclonal Science, Inc.Inventors: Zhenyu Zhu, Dapeng Sun, Weiling Peng
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Patent number: 11155795Abstract: The invention provides for systems, methods, and compositions for altering expression of target gene sequences and related gene products. Provided are structural information on the Cas protein of the CRISPR-Cas system, use of this information in generating modified components of the CRISPR complex, vectors and vector systems which encode one or more components or modified components of a CRISPR complex, as well as methods for the design and use of such vectors and components. Also provided are methods of directing CRISPR complex formation in eukaryotic cells and methods for utilizing the CRISPR-Cas system.Type: GrantFiled: June 2, 2016Date of Patent: October 26, 2021Assignees: THE BROAD INSTITUTE, INC., MASSACHUSETTS INSTITUTE OF TECHNOLOGY, UNIVERSITY OF TOKYOInventors: Feng Zhang, Osamu Nureki, Hiroshi Nishimasu, Ryuichiro Ishitani
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Patent number: 11154574Abstract: Genetically modified compositions, such as non-viral vectors and tumor infiltrating lymphocytes, for the treatment of gastrointestinal cancer are disclosed. Disclosed are methods of utilizing a CRISPR system to generate genetically modified compositions. Also disclosed are the methods of making and using the genetically modified compositions for the treatment of gastrointestinal cancer.Type: GrantFiled: May 28, 2020Date of Patent: October 26, 2021Assignees: Regents of the University of Minnesota, Intima Bioscience, Inc., The United States of America, as Represented by the Secretary, Department of Health and Human ServicesInventors: Branden Moriarity, Beau Webber, Modassir Choudhry, Steven A. Rosenberg, Douglas C. Palmer, Nicholas P. Restifo
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Patent number: 11149297Abstract: The invention relates to methods of depleting RNA from a nucleic acid sample. The RNA may be any RNA, including, but not limited to, rRNA, tRNA, and mRNA. The method is useful for depleting RNA from a nucleic acid sample obtained from a fixed paraffin-embedded tissue (FPET) sample. The method may also be used to prepare cDNA, in particular, a cDNA library for further analysis or manipulation.Type: GrantFiled: January 10, 2018Date of Patent: October 19, 2021Assignee: Genomic Health, Inc.Inventors: Dominick Sinicropi, John Morlan
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Patent number: 11142788Abstract: The invention provides methods of isolating a target nucleic acid in a sample. A primer is hybridized to the target. A polymerase and modified nucleotide resistant to nuclease degradation are used to extend the primer to create a modified polynucleotide. The sample is exposed to a nuclease, thereby isolating the modified polynucleotide. Optionally, the target nucleic acid may be further protected by binding a protein in a sequence specific manner to one end of the target nucleic acid to create a protected target nucleic acid resistant to nuclease degradation. Thus, after exposing the sample to a nuclease, the modified polynucleotide and protected target nucleic acid are isolated.Type: GrantFiled: June 13, 2018Date of Patent: October 12, 2021Assignee: GENETICS RESEARCH, LLCInventor: William Glover
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Patent number: 11136565Abstract: Provided herein are modified Archaeal family B polymerases derived from the Archaeal microorganism Pyrococcus abyssi that exhibit improved incorporation of nucleotide analogues utilized in DNA sequencing.Type: GrantFiled: September 11, 2019Date of Patent: October 5, 2021Assignee: SINGULAR GENOMICS SYSTEMS, INC.Inventors: Souad Naji, Carl W. Fuller, Eli Glezer, Andrew Spaventa
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Patent number: 11118169Abstract: This disclosure provides peptides, polypeptides, fusion polypeptides, compositions, and methods for enhancing or increasing the stability of a polypeptide (e.g., Taq polymerase). Such peptides, polypeptides, fusion polypeptides, or compositions include polypeptides linked to a peptide tag that enhances the stability of the polypeptide. The peptides, polypeptides, fusion polypeptides, compositions may also enhance the activity, specificity, and/or fidelity of other polypeptides in a reaction mixture. The disclosure also provides methods of using such peptides, polypeptides, fusion polypeptides, compositions.Type: GrantFiled: September 13, 2019Date of Patent: September 14, 2021Assignee: Solis BioDyne OÜInventors: Olev Kahre, Kadri Artma, Tiina Kahre
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Patent number: 11091768Abstract: The present disclosure provides genetic constructs containing a promoter that is useful in driving fruit-specific expression in plants. Further provided are expression vectors, transgenic plants and plant parts containing such genetic constructs, as well as uses thereof.Type: GrantFiled: May 23, 2019Date of Patent: August 17, 2021Assignee: The United States of America, as represented by the Secretary of AgricultureInventors: James G. Thomson, Roger L. Thilmony, Kasturi Dasgupta, Christopher D. Dardick
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Patent number: 11091746Abstract: The present disclosure provides compositions, methods, kits, systems and apparatus that are useful for nucleic acid polymerization. In particular, modified polymerases and biologically active fragments thereof are provided that allow for nucleic acid amplification. In some aspects, the disclosure provides modified polymerases having lower systematic error as compared to a reference polymerase. In one aspect, the disclosure relates to modified polymerases useful for nucleic acid sequencing, genotyping, copy number variation analysis, paired-end sequencing and other forms of genetic analysis. In some aspects, the disclosure relates to modified polymerases useful for the generation of nucleic acid libraries or nucleic acid templates. In some aspects, the disclosure relates to the identification of homologous amino acid mutations that can be transferred across classes or families of polymerases to provide novel polymerases with altered properties.Type: GrantFiled: June 5, 2020Date of Patent: August 17, 2021Assignee: Life Technologies CorporationInventors: Peter Vander Horn, Daniel Mazur, Theo Nikiforov, Mindy Landes, Eileen Tozer
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Patent number: 11046726Abstract: The present invention provides methods, compositions, mixtures and kits utilizing deoxynucleoside triphosphates comprising a 3?-O position capped by a group comprising methylenedisulfide as a cleavable protecting group and a detectable label reversibly connected to the nucleobase of said deoxynucleoside. Such compounds provide new possibilities for future sequencing technologies, including but not limited to Sequencing by Synthesis.Type: GrantFiled: March 22, 2018Date of Patent: June 29, 2021Assignee: QIAGEN SCIENCES, LLCInventors: Mong Sano Marma, Jerzy Olejnik, Ilia Korboukh
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Patent number: 10975415Abstract: A polypeptide having a mismatch endonuclease activity of recognizing a mismatch and cleaving the mismatch; a mismatch-specific cleavage reaction using the polypeptide; a method for removing an error in a nucleic acid amplification reaction utilizing the polypeptide; a method for inhibiting the amplification of a nucleic acid comprising a specific nucleotide sequence during a nucleic acid amplification reaction; and a method for detecting a nucleic acid having a single-nucleotide polymorphism mutation utilizing the inhibition method.Type: GrantFiled: September 9, 2015Date of Patent: April 13, 2021Assignees: TAKARA BIO INC., KYUSHU UNIVERSITY, NATIONAL UNIVERSITY CORPORATION, EDUCATIONAL CORPORATION KANSAI BUNRI SOUGOUGAKUENInventors: Takashi Uemori, Yoshizumi Ishino, Takehiro Sagara, Sonoko Ishino, Takeshi Yamagami, Tsuyoshi Shirai
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Patent number: 10935546Abstract: The present invention deals with heterotrimeric AMP-activated protein kinase (AMPK) comprising a fluorophore pair wherein the conformational change can be measured by FRET. It represents an advanced tool to screen and identify AMPK interactors in vitro and in cells in vivo. Such invention can also be considered as a reporter of the cellular energy status as it allows the spatiotemporal monitoring, in situ, of fluctuations in the ratio of AMP and ADP versus ATP.Type: GrantFiled: June 18, 2015Date of Patent: March 2, 2021Assignees: Universite Grenoble Alpes, European Molecular Biology LaboratoryInventors: Imre Berger, Martin Pelosse, Uwe Schlattner
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Patent number: 10920253Abstract: Described is a method for the enzymatic production of acetyl phosphate from formaldehyde using a phosphoketolase or a sulfoacetaldehyde acetyltransferase.Type: GrantFiled: March 13, 2015Date of Patent: February 16, 2021Assignee: Scientist of Fortune, S.A.Inventor: Philippe Marliere
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Patent number: 10900059Abstract: Provided are improved methods for identifying the substrate recognition specificity or activity of a protease, convertase (sortase), or kinase. In some embodiments, methods are provided for identifying the endogenous protease or convertase cleaving patterns (e.g., “cleaveOme”) inside the secretory pathway of a living cell. Select embodiments involve aspects of yeast endoplasmic reticulum sequestration screening and next generation sequencing. Methods of producing polypeptides in Kex2 knockout yeast are also provided.Type: GrantFiled: October 14, 2015Date of Patent: January 26, 2021Assignee: Research Development FoundationInventors: Brent Iverson, Peter Marek, Joseph Taft
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Patent number: 10844430Abstract: The present invention provides methods, compositions, mixtures and kits utilizing 5-Chloro-2-methyl-4-isothiazolin-3-one in sequencing reactions, and in particular, sequencing reactions where deoxynucleoside triphosphates comprising a 3?-O position capped by a disulfide-based 3?-terminator group are used. In one embodiment, the deoxynucleoside triphosphates comprise a 3?-O position capped by a group comprising methylenedisulfide as a cleavable protecting group and a detectable label reversibly connected to the nucleobase of said deoxynucleoside. In addition, thiol-containing compounds and scavengers of thio-containing compounds are described. Such compounds provide new possibilities for future sequencing technologies, including but not limited to Sequencing by Synthesis.Type: GrantFiled: January 24, 2018Date of Patent: November 24, 2020Assignee: Qiagen Sciences, LLCInventors: Luisa Andruzzi, Jimmy Adediran, Timothy Pelletier, Austin Ricker, Angela DeLucia, Ben McNally, Dona Hevroni, Mong Sano Marma, John Andrew Sheridan
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Patent number: 10822420Abstract: The present invention provides a non-human animal in which a DNA comprising an hp7 sequence-encoding DNA and a poly A addition signal-encoding DNA added on the 3? side of a DNA encoding an arbitrary foreign gene is inserted in the same reading frame as that of an arbitrary target gene present on the genome of the non-human animal.Type: GrantFiled: September 13, 2013Date of Patent: November 3, 2020Assignee: CHUGAI SEIYAKU KABUSHIKI KAISHAInventors: Koichi Jishage, Otoya Ueda
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Patent number: 10792340Abstract: Provided is an antibacterial composition against carbapenem-resistant gram-negative bacteria which includes, as an active ingredient, adenylate kinase (ADK) protein derived from Mycobacterium tuberculosis. The ADK protein derived from Mycobacterium tuberculosis, according to the subject matter, has excellent antimicrobial activity against carbapenem-resistant gram-negative bacteria, and thus may be usefully used in a variety of fields as an antibacterial composition.Type: GrantFiled: April 4, 2016Date of Patent: October 6, 2020Assignee: KONKUK UNIVERSITY GLOCAL INDUSTRY-ACADEMIC COLLABORATION FOUNDATIONInventors: Yeong Min Park, In Duk Jung, Seung Jun Lee
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Patent number: 10787669Abstract: The present disclosure relates generally to compounds comprising oligonucleotides complementary to a Leucine-Rich-Repeat-Kinase (LRRK2) RNA transcript. Certain such compounds are useful for hybridizing to a LRRK2 RNA transcript, including but not limited to a LRRK2 RNA transcript in a cell. In certain embodiments, such hybridization results in modulation of splicing of the LRRK2 transcript. In certain embodiments, such compounds are used to treat one or more symptoms associated with Parkinson's disease.Type: GrantFiled: June 11, 2019Date of Patent: September 29, 2020Assignees: Rosalind Franklin University of Medicine and Science, The McLean Hospital CorporationInventors: Michelle L. Hastings, Ole Isacson, Joanna A. Korecka-Roet
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Patent number: 10689688Abstract: Methods and systems for detecting chemical and biological agents using oligonucleotide aptamers. A sensor includes a detection aptamer that has a binding domain for the chemical or biological agent, and is bound to fibers of a textile such as a patch or article of clothing. The detection aptamer can be stabilized and enhanced through a stabilization agent such as trehalose or through binding to a nanoparticle which is then bound to the fiber. Binding of the chemical and biological agent of interest to the detection aptamer can be reported to the user or wearer of the textile in a variety of ways, including visually and electrically.Type: GrantFiled: May 8, 2018Date of Patent: June 23, 2020Assignee: SRC, Inc.Inventor: Adam J. Lowe
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Patent number: 10683537Abstract: Provided herein are mutant polymerase enzymes resistant to inhibitors encountered in Polymerase Chain Reactions (PCR). Also provided are nucleic acids or constructs encoding isolated polypeptides having polymerase activity. Also provided are kits useful for PCR containing isolated polypeptides having polymerase activity or isolated nucleic acids encoding such.Type: GrantFiled: October 16, 2013Date of Patent: June 16, 2020Assignee: DNA Polymerase Technology, Inc.Inventors: Milko B. Kermekchiev, Zhian Zhang
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Patent number: 10676724Abstract: The present disclosure provides compositions, methods, kits, systems and apparatus that are useful for nucleic acid polymerization. In particular, modified polymerases and biologically active fragments thereof are provided that allow for nucleic acid amplification. In some aspects, the disclosure provides modified polymerases having lower systematic error as compared to a reference polymerase. In one aspect, the disclosure relates to modified polymerases useful for nucleic acid sequencing, genotyping, copy number variation analysis, paired-end sequencing and other forms of genetic analysis. In some aspects, the disclosure relates to modified polymerases useful for the generation of nucleic acid libraries or nucleic acid templates. In some aspects, the disclosure relates to the identification of homologous amino acid mutations that can be transferred across classes or families of polymerases to provide novel polymerases with altered properties.Type: GrantFiled: February 7, 2019Date of Patent: June 9, 2020Assignee: LIFE TECHNOLOGIES CORPORATIONInventors: Peter Vander Horn, Daniel Mazur, Theo Nikiforov, Mindy Landes, Eileen Tozer
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Patent number: 10669320Abstract: Provided herein are compositions and methods for the treatment of cancer by activating the spindle assembly checkpoint (SAC) in cells. In particular, dimerized Mps1 and Spc105/KNL1 constructs are provided as tunable activators of SAC, allowing for control of chromosome segregation accuracy and prevention of aneuploidies that are common in cancer.Type: GrantFiled: November 18, 2016Date of Patent: June 2, 2020Assignee: THE REGENTS OF THE UNIVERSITY OF MICHIGANInventors: Ajit P. Joglekar, Pavithra Aravamudhan
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Patent number: 10655187Abstract: The present invention relates to a gene for predicting or diagnosing the prognosis of early-stage breast cancer and to a use thereof, and more specifically relates to a genetic marker for predicting or diagnosing the prognosis of breast cancer, including TRBC1 (T cell receptor beta constant 1), BTN3A2 (butyrophilin, subfamily 3, member A2) or HLA-DPA1 (major histocompatibility complex, class II, DP alpha 1) for providing information necessary for predicting or diagnosing the prognosis of a breast cancer patient. The genetic marker of the present invention allows the prediction or diagnosis of the prognosis of a breast cancer patient, and can therefore advantageously be used for the purpose of providing a direction as to the future course of breast cancer treatment, including a decision on whether anticancer therapy is necessary.Type: GrantFiled: October 9, 2018Date of Patent: May 19, 2020Assignee: GENCURIX INC.Inventors: Young Kee Shin, Young-Deug Kim, En Sel Oh, Jun Young Choi, Sang Rea Cho
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Patent number: 10655161Abstract: Provided herein are methods for the diagnosis, prognosis, or management of diseases, such as cancer, by measuring the tyrosine kinase activity in acellular body fluids. Further provided are methods of predicting response to therapy in certain populations of cancer patients by contacting an acellular body fluid sample from a patient with a test agent, such as a tyrosine kinase inhibitor, and then measuring the effect of the test agent on tyrosine kinase activity in the sample.Type: GrantFiled: December 3, 2014Date of Patent: May 19, 2020Assignee: Quest Diagnostics Investments IncorporatedInventor: Chen-Hsiung Yeh