Transferring Phosphorus Containing Group (e.g., Kineases, Etc.(2.7)) Patents (Class 435/194)
  • Patent number: 11180740
    Abstract: Reaction compositions are disclosed herein comprising at least water, alpha-glucose-1-phosphate (alpha-G1P), an acceptor molecule, and a beta-1,3-glucan phosphorylase enzyme. These reactions can synthesize oligosaccharides and polysaccharides with beta-1,3 glycosidic linkages. Further disclosed are methods of isolating beta-1,3-glucan.
    Type: Grant
    Filed: April 15, 2019
    Date of Patent: November 23, 2021
    Assignee: DANISCO US INC
    Inventors: Zheyong Yu, Slavko Kralj, Natnael Behabtu, Zhenghong Zhang, Laurie A Howe
  • Patent number: 11168312
    Abstract: The invention includes a mutant Taq polymerase, which can effectively amplify a target sequence under conditions of salt concentration(s) similar to body fluids, including blood, serum or plasma preserved with sodium citrate. The mutant Taq polymerase, or a biologically active fragment thereof, has one or more substitutions differing from the wild type as shown in Table I.
    Type: Grant
    Filed: March 9, 2020
    Date of Patent: November 9, 2021
    Assignee: ABclonal Science, Inc.
    Inventors: Zhenyu Zhu, Dapeng Sun, Weiling Peng
  • Patent number: 11154574
    Abstract: Genetically modified compositions, such as non-viral vectors and tumor infiltrating lymphocytes, for the treatment of gastrointestinal cancer are disclosed. Disclosed are methods of utilizing a CRISPR system to generate genetically modified compositions. Also disclosed are the methods of making and using the genetically modified compositions for the treatment of gastrointestinal cancer.
    Type: Grant
    Filed: May 28, 2020
    Date of Patent: October 26, 2021
    Assignees: Regents of the University of Minnesota, Intima Bioscience, Inc., The United States of America, as Represented by the Secretary, Department of Health and Human Services
    Inventors: Branden Moriarity, Beau Webber, Modassir Choudhry, Steven A. Rosenberg, Douglas C. Palmer, Nicholas P. Restifo
  • Patent number: 11155795
    Abstract: The invention provides for systems, methods, and compositions for altering expression of target gene sequences and related gene products. Provided are structural information on the Cas protein of the CRISPR-Cas system, use of this information in generating modified components of the CRISPR complex, vectors and vector systems which encode one or more components or modified components of a CRISPR complex, as well as methods for the design and use of such vectors and components. Also provided are methods of directing CRISPR complex formation in eukaryotic cells and methods for utilizing the CRISPR-Cas system.
    Type: Grant
    Filed: June 2, 2016
    Date of Patent: October 26, 2021
    Assignees: THE BROAD INSTITUTE, INC., MASSACHUSETTS INSTITUTE OF TECHNOLOGY, UNIVERSITY OF TOKYO
    Inventors: Feng Zhang, Osamu Nureki, Hiroshi Nishimasu, Ryuichiro Ishitani
  • Patent number: 11149297
    Abstract: The invention relates to methods of depleting RNA from a nucleic acid sample. The RNA may be any RNA, including, but not limited to, rRNA, tRNA, and mRNA. The method is useful for depleting RNA from a nucleic acid sample obtained from a fixed paraffin-embedded tissue (FPET) sample. The method may also be used to prepare cDNA, in particular, a cDNA library for further analysis or manipulation.
    Type: Grant
    Filed: January 10, 2018
    Date of Patent: October 19, 2021
    Assignee: Genomic Health, Inc.
    Inventors: Dominick Sinicropi, John Morlan
  • Patent number: 11142788
    Abstract: The invention provides methods of isolating a target nucleic acid in a sample. A primer is hybridized to the target. A polymerase and modified nucleotide resistant to nuclease degradation are used to extend the primer to create a modified polynucleotide. The sample is exposed to a nuclease, thereby isolating the modified polynucleotide. Optionally, the target nucleic acid may be further protected by binding a protein in a sequence specific manner to one end of the target nucleic acid to create a protected target nucleic acid resistant to nuclease degradation. Thus, after exposing the sample to a nuclease, the modified polynucleotide and protected target nucleic acid are isolated.
    Type: Grant
    Filed: June 13, 2018
    Date of Patent: October 12, 2021
    Assignee: GENETICS RESEARCH, LLC
    Inventor: William Glover
  • Patent number: 11136565
    Abstract: Provided herein are modified Archaeal family B polymerases derived from the Archaeal microorganism Pyrococcus abyssi that exhibit improved incorporation of nucleotide analogues utilized in DNA sequencing.
    Type: Grant
    Filed: September 11, 2019
    Date of Patent: October 5, 2021
    Assignee: SINGULAR GENOMICS SYSTEMS, INC.
    Inventors: Souad Naji, Carl W. Fuller, Eli Glezer, Andrew Spaventa
  • Patent number: 11118169
    Abstract: This disclosure provides peptides, polypeptides, fusion polypeptides, compositions, and methods for enhancing or increasing the stability of a polypeptide (e.g., Taq polymerase). Such peptides, polypeptides, fusion polypeptides, or compositions include polypeptides linked to a peptide tag that enhances the stability of the polypeptide. The peptides, polypeptides, fusion polypeptides, compositions may also enhance the activity, specificity, and/or fidelity of other polypeptides in a reaction mixture. The disclosure also provides methods of using such peptides, polypeptides, fusion polypeptides, compositions.
    Type: Grant
    Filed: September 13, 2019
    Date of Patent: September 14, 2021
    Assignee: Solis BioDyne OÜ
    Inventors: Olev Kahre, Kadri Artma, Tiina Kahre
  • Patent number: 11091768
    Abstract: The present disclosure provides genetic constructs containing a promoter that is useful in driving fruit-specific expression in plants. Further provided are expression vectors, transgenic plants and plant parts containing such genetic constructs, as well as uses thereof.
    Type: Grant
    Filed: May 23, 2019
    Date of Patent: August 17, 2021
    Assignee: The United States of America, as represented by the Secretary of Agriculture
    Inventors: James G. Thomson, Roger L. Thilmony, Kasturi Dasgupta, Christopher D. Dardick
  • Patent number: 11091746
    Abstract: The present disclosure provides compositions, methods, kits, systems and apparatus that are useful for nucleic acid polymerization. In particular, modified polymerases and biologically active fragments thereof are provided that allow for nucleic acid amplification. In some aspects, the disclosure provides modified polymerases having lower systematic error as compared to a reference polymerase. In one aspect, the disclosure relates to modified polymerases useful for nucleic acid sequencing, genotyping, copy number variation analysis, paired-end sequencing and other forms of genetic analysis. In some aspects, the disclosure relates to modified polymerases useful for the generation of nucleic acid libraries or nucleic acid templates. In some aspects, the disclosure relates to the identification of homologous amino acid mutations that can be transferred across classes or families of polymerases to provide novel polymerases with altered properties.
    Type: Grant
    Filed: June 5, 2020
    Date of Patent: August 17, 2021
    Assignee: Life Technologies Corporation
    Inventors: Peter Vander Horn, Daniel Mazur, Theo Nikiforov, Mindy Landes, Eileen Tozer
  • Patent number: 11046726
    Abstract: The present invention provides methods, compositions, mixtures and kits utilizing deoxynucleoside triphosphates comprising a 3?-O position capped by a group comprising methylenedisulfide as a cleavable protecting group and a detectable label reversibly connected to the nucleobase of said deoxynucleoside. Such compounds provide new possibilities for future sequencing technologies, including but not limited to Sequencing by Synthesis.
    Type: Grant
    Filed: March 22, 2018
    Date of Patent: June 29, 2021
    Assignee: QIAGEN SCIENCES, LLC
    Inventors: Mong Sano Marma, Jerzy Olejnik, Ilia Korboukh
  • Patent number: 10975415
    Abstract: A polypeptide having a mismatch endonuclease activity of recognizing a mismatch and cleaving the mismatch; a mismatch-specific cleavage reaction using the polypeptide; a method for removing an error in a nucleic acid amplification reaction utilizing the polypeptide; a method for inhibiting the amplification of a nucleic acid comprising a specific nucleotide sequence during a nucleic acid amplification reaction; and a method for detecting a nucleic acid having a single-nucleotide polymorphism mutation utilizing the inhibition method.
    Type: Grant
    Filed: September 9, 2015
    Date of Patent: April 13, 2021
    Assignees: TAKARA BIO INC., KYUSHU UNIVERSITY, NATIONAL UNIVERSITY CORPORATION, EDUCATIONAL CORPORATION KANSAI BUNRI SOUGOUGAKUEN
    Inventors: Takashi Uemori, Yoshizumi Ishino, Takehiro Sagara, Sonoko Ishino, Takeshi Yamagami, Tsuyoshi Shirai
  • Patent number: 10935546
    Abstract: The present invention deals with heterotrimeric AMP-activated protein kinase (AMPK) comprising a fluorophore pair wherein the conformational change can be measured by FRET. It represents an advanced tool to screen and identify AMPK interactors in vitro and in cells in vivo. Such invention can also be considered as a reporter of the cellular energy status as it allows the spatiotemporal monitoring, in situ, of fluctuations in the ratio of AMP and ADP versus ATP.
    Type: Grant
    Filed: June 18, 2015
    Date of Patent: March 2, 2021
    Assignees: Universite Grenoble Alpes, European Molecular Biology Laboratory
    Inventors: Imre Berger, Martin Pelosse, Uwe Schlattner
  • Patent number: 10920253
    Abstract: Described is a method for the enzymatic production of acetyl phosphate from formaldehyde using a phosphoketolase or a sulfoacetaldehyde acetyltransferase.
    Type: Grant
    Filed: March 13, 2015
    Date of Patent: February 16, 2021
    Assignee: Scientist of Fortune, S.A.
    Inventor: Philippe Marliere
  • Patent number: 10900059
    Abstract: Provided are improved methods for identifying the substrate recognition specificity or activity of a protease, convertase (sortase), or kinase. In some embodiments, methods are provided for identifying the endogenous protease or convertase cleaving patterns (e.g., “cleaveOme”) inside the secretory pathway of a living cell. Select embodiments involve aspects of yeast endoplasmic reticulum sequestration screening and next generation sequencing. Methods of producing polypeptides in Kex2 knockout yeast are also provided.
    Type: Grant
    Filed: October 14, 2015
    Date of Patent: January 26, 2021
    Assignee: Research Development Foundation
    Inventors: Brent Iverson, Peter Marek, Joseph Taft
  • Patent number: 10844430
    Abstract: The present invention provides methods, compositions, mixtures and kits utilizing 5-Chloro-2-methyl-4-isothiazolin-3-one in sequencing reactions, and in particular, sequencing reactions where deoxynucleoside triphosphates comprising a 3?-O position capped by a disulfide-based 3?-terminator group are used. In one embodiment, the deoxynucleoside triphosphates comprise a 3?-O position capped by a group comprising methylenedisulfide as a cleavable protecting group and a detectable label reversibly connected to the nucleobase of said deoxynucleoside. In addition, thiol-containing compounds and scavengers of thio-containing compounds are described. Such compounds provide new possibilities for future sequencing technologies, including but not limited to Sequencing by Synthesis.
    Type: Grant
    Filed: January 24, 2018
    Date of Patent: November 24, 2020
    Assignee: Qiagen Sciences, LLC
    Inventors: Luisa Andruzzi, Jimmy Adediran, Timothy Pelletier, Austin Ricker, Angela DeLucia, Ben McNally, Dona Hevroni, Mong Sano Marma, John Andrew Sheridan
  • Patent number: 10822420
    Abstract: The present invention provides a non-human animal in which a DNA comprising an hp7 sequence-encoding DNA and a poly A addition signal-encoding DNA added on the 3? side of a DNA encoding an arbitrary foreign gene is inserted in the same reading frame as that of an arbitrary target gene present on the genome of the non-human animal.
    Type: Grant
    Filed: September 13, 2013
    Date of Patent: November 3, 2020
    Assignee: CHUGAI SEIYAKU KABUSHIKI KAISHA
    Inventors: Koichi Jishage, Otoya Ueda
  • Patent number: 10792340
    Abstract: Provided is an antibacterial composition against carbapenem-resistant gram-negative bacteria which includes, as an active ingredient, adenylate kinase (ADK) protein derived from Mycobacterium tuberculosis. The ADK protein derived from Mycobacterium tuberculosis, according to the subject matter, has excellent antimicrobial activity against carbapenem-resistant gram-negative bacteria, and thus may be usefully used in a variety of fields as an antibacterial composition.
    Type: Grant
    Filed: April 4, 2016
    Date of Patent: October 6, 2020
    Assignee: KONKUK UNIVERSITY GLOCAL INDUSTRY-ACADEMIC COLLABORATION FOUNDATION
    Inventors: Yeong Min Park, In Duk Jung, Seung Jun Lee
  • Patent number: 10787669
    Abstract: The present disclosure relates generally to compounds comprising oligonucleotides complementary to a Leucine-Rich-Repeat-Kinase (LRRK2) RNA transcript. Certain such compounds are useful for hybridizing to a LRRK2 RNA transcript, including but not limited to a LRRK2 RNA transcript in a cell. In certain embodiments, such hybridization results in modulation of splicing of the LRRK2 transcript. In certain embodiments, such compounds are used to treat one or more symptoms associated with Parkinson's disease.
    Type: Grant
    Filed: June 11, 2019
    Date of Patent: September 29, 2020
    Assignees: Rosalind Franklin University of Medicine and Science, The McLean Hospital Corporation
    Inventors: Michelle L. Hastings, Ole Isacson, Joanna A. Korecka-Roet
  • Patent number: 10689688
    Abstract: Methods and systems for detecting chemical and biological agents using oligonucleotide aptamers. A sensor includes a detection aptamer that has a binding domain for the chemical or biological agent, and is bound to fibers of a textile such as a patch or article of clothing. The detection aptamer can be stabilized and enhanced through a stabilization agent such as trehalose or through binding to a nanoparticle which is then bound to the fiber. Binding of the chemical and biological agent of interest to the detection aptamer can be reported to the user or wearer of the textile in a variety of ways, including visually and electrically.
    Type: Grant
    Filed: May 8, 2018
    Date of Patent: June 23, 2020
    Assignee: SRC, Inc.
    Inventor: Adam J. Lowe
  • Patent number: 10683537
    Abstract: Provided herein are mutant polymerase enzymes resistant to inhibitors encountered in Polymerase Chain Reactions (PCR). Also provided are nucleic acids or constructs encoding isolated polypeptides having polymerase activity. Also provided are kits useful for PCR containing isolated polypeptides having polymerase activity or isolated nucleic acids encoding such.
    Type: Grant
    Filed: October 16, 2013
    Date of Patent: June 16, 2020
    Assignee: DNA Polymerase Technology, Inc.
    Inventors: Milko B. Kermekchiev, Zhian Zhang
  • Patent number: 10676724
    Abstract: The present disclosure provides compositions, methods, kits, systems and apparatus that are useful for nucleic acid polymerization. In particular, modified polymerases and biologically active fragments thereof are provided that allow for nucleic acid amplification. In some aspects, the disclosure provides modified polymerases having lower systematic error as compared to a reference polymerase. In one aspect, the disclosure relates to modified polymerases useful for nucleic acid sequencing, genotyping, copy number variation analysis, paired-end sequencing and other forms of genetic analysis. In some aspects, the disclosure relates to modified polymerases useful for the generation of nucleic acid libraries or nucleic acid templates. In some aspects, the disclosure relates to the identification of homologous amino acid mutations that can be transferred across classes or families of polymerases to provide novel polymerases with altered properties.
    Type: Grant
    Filed: February 7, 2019
    Date of Patent: June 9, 2020
    Assignee: LIFE TECHNOLOGIES CORPORATION
    Inventors: Peter Vander Horn, Daniel Mazur, Theo Nikiforov, Mindy Landes, Eileen Tozer
  • Patent number: 10669320
    Abstract: Provided herein are compositions and methods for the treatment of cancer by activating the spindle assembly checkpoint (SAC) in cells. In particular, dimerized Mps1 and Spc105/KNL1 constructs are provided as tunable activators of SAC, allowing for control of chromosome segregation accuracy and prevention of aneuploidies that are common in cancer.
    Type: Grant
    Filed: November 18, 2016
    Date of Patent: June 2, 2020
    Assignee: THE REGENTS OF THE UNIVERSITY OF MICHIGAN
    Inventors: Ajit P. Joglekar, Pavithra Aravamudhan
  • Patent number: 10655187
    Abstract: The present invention relates to a gene for predicting or diagnosing the prognosis of early-stage breast cancer and to a use thereof, and more specifically relates to a genetic marker for predicting or diagnosing the prognosis of breast cancer, including TRBC1 (T cell receptor beta constant 1), BTN3A2 (butyrophilin, subfamily 3, member A2) or HLA-DPA1 (major histocompatibility complex, class II, DP alpha 1) for providing information necessary for predicting or diagnosing the prognosis of a breast cancer patient. The genetic marker of the present invention allows the prediction or diagnosis of the prognosis of a breast cancer patient, and can therefore advantageously be used for the purpose of providing a direction as to the future course of breast cancer treatment, including a decision on whether anticancer therapy is necessary.
    Type: Grant
    Filed: October 9, 2018
    Date of Patent: May 19, 2020
    Assignee: GENCURIX INC.
    Inventors: Young Kee Shin, Young-Deug Kim, En Sel Oh, Jun Young Choi, Sang Rea Cho
  • Patent number: 10655161
    Abstract: Provided herein are methods for the diagnosis, prognosis, or management of diseases, such as cancer, by measuring the tyrosine kinase activity in acellular body fluids. Further provided are methods of predicting response to therapy in certain populations of cancer patients by contacting an acellular body fluid sample from a patient with a test agent, such as a tyrosine kinase inhibitor, and then measuring the effect of the test agent on tyrosine kinase activity in the sample.
    Type: Grant
    Filed: December 3, 2014
    Date of Patent: May 19, 2020
    Assignee: Quest Diagnostics Investments Incorporated
    Inventor: Chen-Hsiung Yeh
  • Patent number: 10633641
    Abstract: The present disclosure provides compositions, methods, kits, systems and apparatus that are useful for nucleic acid polymerization. In particular, modified polymerases and biologically active fragment thereof are provided that allow for nucleic acid amplification. In one aspect, the disclosure relates to modified polymerases useful for nucleic acid sequencing, genotyping, copy number variation analysis, paired-end sequencing and other forms of genetic analysis. In some aspects, the disclosure relates to modified polymerases useful for the generation of nucleic acid libraries or nucleic acid templates for use in various downstream processes. In some aspects, the disclosure relates to the identification of homologous amino acid mutations that can be transferred across classes or families of polymerases to provide novel polymerases with altered catalytic properties. In some aspects, the disclosure provides modified polymerases having enhanced catalytic properties as compared to a reference polymerase.
    Type: Grant
    Filed: April 23, 2018
    Date of Patent: April 28, 2020
    Assignee: Life Technologies Corporation
    Inventors: Peter Vander Horn, Theo Nikiforov, Guobin Luo, Mindy Landes, Daniel Mazur, Eileen Tozer, Tommie Lloyd Lincecum
  • Patent number: 10638168
    Abstract: A method, computer system, and a computer program product for detecting a minimum viable display resolution is provided. The present invention may include measuring a first quality indicator of a received video file in an original resolution of the received video file. The present invention may then include measuring a second quality indicator of the received video file in a newest resolution of the received video file, wherein the newest resolution of the received video file is a lower resolution relative to the original resolution of the received video file. The present invention may further include, in response to determining that the measured second quality indicator of the newest resolution does not meet a threshold associated with the measured first quality indicator of the original resolution, discarding the newest resolution of the received video file and storing at least one viable display resolution of the received video file.
    Type: Grant
    Filed: September 6, 2018
    Date of Patent: April 28, 2020
    Assignee: International Business Machines Corporation
    Inventors: Jack E. Dunning, Thomas J. Latham, Jordan R. Cain
  • Patent number: 10604746
    Abstract: The present disclosure provides engineered RNA-guided enzymes for editing live cells.
    Type: Grant
    Filed: October 21, 2019
    Date of Patent: March 31, 2020
    Assignee: Inscripta, Inc.
    Inventors: Benjamin Mijts, Juhan Kim, Aamir Mir, Kyle Seamon, Andrew Garst
  • Patent number: 10501813
    Abstract: Disclosed is a thermostable DNA polymerase preparation which can illimitably reduce the risk of false positivity in the detection of a subject microorganism utilizing a gene amplification reaction and therefore enables the selective amplification of DNA for detecting the subject microorganism even when the amount of the subject microorganism is small and therefore the amount of DNA collected therefrom is extremely small, and can be produced at a reduced cost. Also disclosed is a method for quantifying or quantifying/identifying a subject organism to be detected rapidly, conveniently and with high sensitivity using the preparation of the present invention.
    Type: Grant
    Filed: August 28, 2017
    Date of Patent: December 10, 2019
    Assignees: HOKKAIDO MITSUI CHEMICALS INC., NATIONAL UNIVERSITY CORPORATION UNIVERSITY OF TOYAMA
    Inventors: Homare Tabata, Hiroshi Minami, Hideki Niimi, Isao Kitajima, Tomohiro Ueno, Shiroh Hayashi, Masashi Mori
  • Patent number: 10465175
    Abstract: Provided are a recombinant microorganism including a genetic modification that increases a pyruvate, phosphate dikinase activity, a method of producing cellulose using the same, and a method of producing a microorganism having enhanced cellulose productivity.
    Type: Grant
    Filed: October 26, 2017
    Date of Patent: November 5, 2019
    Assignee: SAMSUNG ELECTRONICS CO., LTD.
    Inventors: Hongsoon Rhee, Jinkyu Kang, Jinhwan Park, Dongsik Yang
  • Patent number: 10450605
    Abstract: A method for determining the sequence of a target nucleic acid, including steps of contacting a target nucleic acid with a polymerase to sequentially remove nucleotide triphosphates from the target nucleic acid, wherein the nucleotide triphosphates that are removed have a variety of different base moieties; and distinguishing the different base moieties for the nucleotide triphosphates that are removed. Also provided is a apparatus including a nanopore positioned in a fluid impermeable barrier to form a passage through which a nucleotide triphosphate can pass from a first fluid reservoir to a second fluid reservoir, and a reaction mix in the first fluid reservoir that includes a polymerase, target nucleic acid having two strands, and pyrophosphorolytic concentration of pyrophosphate.
    Type: Grant
    Filed: December 19, 2017
    Date of Patent: October 22, 2019
    Assignee: ILLUMINA CAMBRIDGE LIMITED
    Inventor: Wouter Meuleman
  • Patent number: 10427162
    Abstract: The present disclosure provides systems, devices and methods associates with processing and analyzing samples for molecular diagnostics. The system may process samples using assay cartridges including sample preparation modules and PCR modules. The system may include thermal cycler modules and optics modules to detect the specific nucleic acid sequences in the samples.
    Type: Grant
    Filed: December 21, 2016
    Date of Patent: October 1, 2019
    Assignee: QuanDx Inc.
    Inventors: Xiaojun Lei, Yuan Yuan, Qian Xu, Paul Fleming, Ronan Hayes, Brian Lewis, Bruce Richardson
  • Patent number: 10392671
    Abstract: A method of examining a water supply for microbial contamination involves contacting a water supply with a novel reagent having high specificity for a Legionella microbial species, wherein said reagent does not cross-react, or minimally cross-reacts, with a microbial species other than Legionella. The method further involves detecting, or measuring, a contaminating concentration of a Legionella species in the water supply. Useful reagents comprise at least one nucleotide sequence primer comprising a primer sequence selected from SEQ ID NO: 3-10 or a combination of such primer sequences. The increased specificity of such reagents permits more sensitive detection of microbial contamination.
    Type: Grant
    Filed: June 24, 2015
    Date of Patent: August 27, 2019
    Assignee: Dow Global Technologies LLC
    Inventors: Paul O. Schook, Bei Yin
  • Patent number: 10358670
    Abstract: A method for the production of nucleic acid encoding a target protein. The method comprises (a) providing an array of RNA or DNA molecules including one or more encoding the target protein; (b) generating a target protein from the array to form RNA-protein or DNA-protein complexes in which the RNA or DNA molecule is non-covalently or covalently bound to the complex; (c) separating the complexes into compartments wherein most or all of the compartments contain no more than one complex; (d) subjecting the complexes to reaction conditions which allow target protein activity; and (e) selecting nucleic acid encoding the target protein on the basis of the activity associated therewith, wherein when the complex is a DNA-protein complex in which the DNA is non-covalently bound, step b) is performed in the absence of separate compartments for each complex.
    Type: Grant
    Filed: April 20, 2018
    Date of Patent: July 23, 2019
    Assignee: THERMO FISHER SCIENTIFIC BALTICS UAB
    Inventors: Arvydas Janulaitis, Remigijus Skirgaila, Dangira Siksniene
  • Patent number: 10336785
    Abstract: The present invention provides methods, compositions, mixtures and kits utilizing deoxynucleoside triphosphates comprising a 3?-O position capped by a group comprising methylenedisulfide as a cleavable protecting group and a detectable label reversibly connected to the nucleobase of said deoxynucleoside. Such compounds provide new possibilities for future sequencing technologies, including but not limited to Sequencing by Synthesis.
    Type: Grant
    Filed: November 4, 2016
    Date of Patent: July 2, 2019
    Assignee: Qiagen Sciences, LLC
    Inventors: Mong Sano Marma, Jerzy Olejnik, Ilia Korboukh
  • Patent number: 10336804
    Abstract: The present invention relates to chimeric immune receptor molecules for reducing or eliminating tumors. The chimeric receptors are composed a C-type lectin-like natural killer cell receptor, or a protein associated therewith, fused to an immune signaling receptor containing an immunoreceptor tyrosine-based activation motif. Methods for using the chimeric receptors are further provided.
    Type: Grant
    Filed: January 20, 2015
    Date of Patent: July 2, 2019
    Assignee: TRUSTEES OF DARTMOUTH COLLEGE
    Inventors: Tong Zhang, Charles L. Sentman
  • Patent number: 10301346
    Abstract: The present invention provides methods, compositions, mixtures and kits utilizing deoxynucleoside triphosphates comprising a 3?-O position capped by a group comprising methylenedisulfide as a cleavable protecting group and a detectable label reversibly connected to the nucleobase of said deoxynucleoside. Such compounds provide new possibilities for future sequencing technologies, including but not limited to Sequencing by Synthesis.
    Type: Grant
    Filed: November 4, 2016
    Date of Patent: May 28, 2019
    Assignee: Qiagen Sciences, LLC
    Inventors: Mong Sano Marma, Jerzy Olejnik, Ilia Korboukh
  • Patent number: 10287349
    Abstract: In accordance with the present invention, there are provided functionally modulated tool receptors which are useful for drug discovery and development. In certain aspects and embodiments as described herein, a sophisticated and powerful approach has been designed that allows the rapid development of enhanced receptors, while simultaneously exploring millions of possibilities for improved properties with respect to such properties as protein expression, homogeneity, stabilization, conformational and activation pathway selectivity, antigenicity, immunogenicity, and the like. Indeed, the new methodology described herein represents a breakthrough by leveraging a full range of combinatorial amino acid replacements, in multiple positions simultaneously, in order to generate modified membrane-spanning proteins.
    Type: Grant
    Filed: October 30, 2015
    Date of Patent: May 14, 2019
    Assignee: ABILITA BIO, INC.
    Inventor: Mauro Mileni
  • Patent number: 10273539
    Abstract: The present invention provides methods, compositions, mixtures and kits utilizing deoxynucleoside triphosphates comprising a 3?-O position capped by a group comprising methylenedisulfide as a cleavable protecting group and a detectable label reversibly connected to the nucleobase of said deoxynucleoside. Such compounds provide new possibilities for future sequencing technologies, including but not limited to Sequencing by Synthesis.
    Type: Grant
    Filed: November 4, 2016
    Date of Patent: April 30, 2019
    Assignee: QIAGEN SCIENCES, LLC
    Inventors: Mong Sano Marma, Jerzy Olejnik
  • Patent number: 10246750
    Abstract: The invention provides to PKN1 gene fusions, PKN1 fusion proteins, and fragments of those genes and polypeptides. The invention further provides methods of diagnosing and treating diseases or disorders associated with PKN1 fusions, such as conditions mediated by aberrant PKN1 expression or activity, or over expression of PKN1.
    Type: Grant
    Filed: June 10, 2015
    Date of Patent: April 2, 2019
    Assignee: Blueprint Medicines Corporation
    Inventors: Nicolas Stransky, Joseph L. Kim
  • Patent number: 10246498
    Abstract: Non-FRET-based fusion protein reporter molecules are provided that can be used to monitor histone modifications in living cells. Transgenic animals, particularly non-human mammals, whose genomes comprise an expression cassette encoding a non-FRET-based fusion protein reporter, are also provided. Methods of using the fusion reporter molecules for diagnosing histone-modification-associated disorders and to identify candidate pharmaceutical agents that effect histone modification in cells and tissues are also provided.
    Type: Grant
    Filed: May 29, 2015
    Date of Patent: April 2, 2019
    Assignee: The Johns Hopkins University
    Inventors: Hans Tomas Bjornsson, Harry Dietz
  • Patent number: 10240195
    Abstract: This disclosure provides systems and methods for attaching nanopore-detectable tags to nucleotides. The disclosure also provides methods for sequencing nucleic acids using the disclosed tagged nucleotides.
    Type: Grant
    Filed: March 23, 2015
    Date of Patent: March 26, 2019
    Assignees: THE TRUSTEES OF COLUMBIA UNIVERSITY IN THE CITY OF NEW YORK, GENIA TECHNOLOGIES, INC.
    Inventors: Carl W. Fuller, Shiv Kumar, Jingyue Ju, Randall Davis, Roger Chen
  • Patent number: 10221442
    Abstract: This disclosure provides methods and compositions for sample processing, particularly for sequencing applications. Included within this disclosure are bead compositions, such as diverse libraries of beads attached to large numbers of oligonucleotides containing barcodes. Often, the beads provides herein are degradable. For example, they may contain disulfide bonds that are susceptible to reducing agents. The methods provided herein include methods of making libraries of barcoded beads as well as methods of combining the beads with a sample, such as by using a microfluidic device.
    Type: Grant
    Filed: June 26, 2014
    Date of Patent: March 5, 2019
    Assignee: 10X GENOMICS, INC.
    Inventors: Benjamin Hindson, Christopher Hindson, Michael Schnall-Levin, Kevin Ness, Mirna Jarosz, Serge Saxonov
  • Patent number: 10207240
    Abstract: Methods and devices are provided for manipulating droplets on a support using surface tension properties, moving the droplets along a predetermined path and merging two droplets together enabling a number of chemical reactions. Disclosed are methods for controlling the droplets volumes. Disclosed are methods and devices for synthesizing at least one oligonucleotide having a predefined sequence. Disclosed are methods and devices for synthesizing and/or assembling at least one polynucleotide product having a predefined sequence from a plurality of different oligonucleotides having a predefined sequence.
    Type: Grant
    Filed: November 3, 2010
    Date of Patent: February 19, 2019
    Assignee: Gen9, Inc.
    Inventors: Joseph Jacobson, Larry Li-Yang Chu, Senthil Ramu
  • Patent number: 10144972
    Abstract: A composition for hot-start reverse transcription reaction and a composition for reverse transcription PCR are disclosed. The composition is obtained by adding pyrophosphate and pyrophosphatase to an aqueous solution containing reaction buffer solution, MgCl2, four kinds of dNTPs, and reverse transcription polymerase in a single reaction tube. The composition for hot-start reverse transcription reaction is obtained by freezing or drying the composition. The composition show increased stability and long-term storage stability. Also, disclosed is a composition that additionally includes DNA polymerase, and, thus, enables a hot-start reverse transcription reaction and a PCR reaction to be sequentially performed. A method for amplifying a nucleic acid by using the composition. The composition of the invention can be conveniently and effectively used in multiplex reverse transcription PCRs or real-time quantitative reverse transcription PCR.
    Type: Grant
    Filed: March 11, 2013
    Date of Patent: December 4, 2018
    Assignee: BIONEER CORPORATION
    Inventors: Han Oh Park, Jun Hee Lee, Sora Choi, Hyun Seo Kim
  • Patent number: 10130667
    Abstract: The present invention relates to a vaccine comprising the insertion of three genes, the TAA/ecdCD40L EA1 and CDA, driven by promoters L-plastin/cytosinedeaminase and CMV as a three gene, three transcription unit oncolytic virus as a conditionally replication competent adenoviral vector which replicates only in tumor cells. In these transcription units, the E1A gene of the adenoviral vector as well as the cytosine deaminase gene are under the control of the L-plastin promoter, while the TAA/ecdCD40L transcription unit is under control of a the CMV promoter.
    Type: Grant
    Filed: April 15, 2016
    Date of Patent: November 20, 2018
    Assignee: MicroVAX, LLC
    Inventor: Albert B. Deisseroth
  • Patent number: 10113156
    Abstract: Stabilized reverse transcriptase fusion proteins including a thermostable reverse transcriptase connected to a stabilizer protein are described. Attaching the stabilizer protein to the thermostable reverse transcriptase stabilizes the fusion protein and can aid in its purification, provide increased solubility, allow for longer storage, or allow the fusion protein to be used under more rigorous conditions such as higher temperature. The stabilized reverse transcriptase fusion protein can also include a linker between the stabilizer protein and the thermostable reverse transcriptase. The stabilized reverse transcriptase fusion proteins are suitable for use in nucleic acid amplification methods such as the reverse transcription polymerase chain reaction and other applications involving cDNA synthesis.
    Type: Grant
    Filed: March 4, 2010
    Date of Patent: October 30, 2018
    Assignee: BOARD OF REGENTS, THE UNIVERSITY OF TEXAS SYSTEM
    Inventors: Alan M. Lambowitz, Sabine Mohr, Georg Mohr, Eman Ghanem
  • Patent number: 10093985
    Abstract: Described herein are methods, compositions and kits directed to the detection of gene dysregulations such as those arising from gene fusions and/or chromosomal abnormalities, e.g., translocations, insertions, inversions and deletions. Samples containing dysregulated gene(s) of interest may show independent expression patterns for the 5? and 3? regions of the gene. The methods, compositions and kits are useful for detecting mutations that cause the differential expression of a 5? portion of a target gene relative to the 3? region of the target gene.
    Type: Grant
    Filed: December 8, 2016
    Date of Patent: October 9, 2018
    Assignee: Quest Diagnostics Investments Incorporated
    Inventors: Heather R. Sanders, Maher Albitar, Aurelia Meloni-Ehrig
  • Patent number: 10081799
    Abstract: This disclosure provides, among other things, a composition comprising: comprising a fusion protein comprising: (a) a DNA polymerase; and (b) a heterologous sequence-specific DNA binding domain. A method for copying a DNA template, as well as a kit for performing the same, are also described.
    Type: Grant
    Filed: February 14, 2017
    Date of Patent: September 25, 2018
    Assignee: New England Biolabs, Inc.
    Inventors: Pei-Chung Hsieh, Luo Sun, Thomas C. Evans, Jr., Theodore B. Davis, Andrew Gardner
  • Patent number: RE47684
    Abstract: Disclosed is a cytoprotective agent for use with respect to ischemic damage, including as an active ingredient a triprenyl phenol compound represented by the following general formula (I), wherein X is —CHY—C(CH3)2Z, Y and Z are each independently —H or —OH, or jointly form a single bond, and R represents a hydrogen atom or a substituent with a molecular weight of 1000 or less.
    Type: Grant
    Filed: April 25, 2017
    Date of Patent: November 5, 2019
    Assignee: TMS CO., LTD.
    Inventors: Honda Kazuo, Terumasa Hashimoto, Keita Shibata, Keiko Hasegawa, Keiji Hasumi