Enzyme (e.g., Ligases (6. ), Etc.), Proenzyme; Compositions Thereof; Process For Preparing, Activating, Inhibiting, Separating, Or Purifying Enzymes Patents (Class 435/183)
  • Patent number: 10829770
    Abstract: Polynucleotides encoding fusion proteins contain a secretable luciferase fused to a modified polypeptide of interest are disclosed. The polypeptide of interest has been modified to remove a native N-terminal secretion sequence and has been replaced by the secretable luciferase. One example of a modified polypeptide of interest is interferon. The polynucleotides and fusion proteins have biotherapeutic, diagnostic, and quality control applications in biotechnological, medical, and veterinary fields. Methods for producing the secretable fusion protein are also disclosed.
    Type: Grant
    Filed: September 16, 2019
    Date of Patent: November 10, 2020
    Assignee: The Government of the United States of America, as represented by the Secretary of Homeland Security
    Inventors: Michael Puckette, Max V. Rasmussen
  • Patent number: 10800848
    Abstract: Disclosed herein are compositions and pharmaceutical formulations that comprise a binding moiety conjugated to a polynucleic acid molecule and a polymer. Also described herein include methods for treating a cancer which utilize a composition or a pharmaceutical formulation comprising a binding moiety conjugated to a polynucleic acid molecule and a polymer.
    Type: Grant
    Filed: September 11, 2018
    Date of Patent: October 13, 2020
    Assignee: AVIDITY BIOSCIENCES, INC.
    Inventors: Andrew John Geall, Venkata Ramana Doppalapudi, David Sai-Ho Chu, Michael Caramian Cochran, Rachel Elizabeth Johns, Palani Balu, Rob Burke, Beatrice Diana Darimont
  • Patent number: 10792341
    Abstract: Methods of treating glycogen storage disease type II, by administering acid ?-glucosidase, are described, as are compositions for use in treatment of glycogen storage disease type II.
    Type: Grant
    Filed: January 19, 2018
    Date of Patent: October 6, 2020
    Assignee: Duke University
    Inventor: Yuan-Tsong Chen
  • Patent number: 10793922
    Abstract: The present invention provides methods for rapidly identifying an RNA viral infection using an isothermal nucleic acid amplification reaction that can be carried out extracted RNA in the context of a crude biological sample.
    Type: Grant
    Filed: October 20, 2015
    Date of Patent: October 6, 2020
    Assignee: ENVIROLOGIX INC.
    Inventors: Lars Peters, Stephen A. Judice, Daniel Shaffer, Breck Parker
  • Patent number: 10787519
    Abstract: Disclosed herein are compositions and pharmaceutical formulations that comprise a binding moiety conjugated to a polynucleic acid molecule and a polymer. Also described herein include methods for treating a cancer which utilize a composition or a pharmaceutical formulation comprising a binding moiety conjugated to a polynucleic acid molecule and a polymer.
    Type: Grant
    Filed: September 11, 2018
    Date of Patent: September 29, 2020
    Assignee: AVIDITY BIOSCIENCES, INC.
    Inventors: Andrew John Geall, Venkata Ramana Doppalapudi, David Sai-Ho Chu, Michael Caramian Cochran, Rachel Elizabeth Johns, Palani Balu, Rob Burke, Beatrice Diana Darimont
  • Patent number: 10786798
    Abstract: The present invention relates to a method for preparing solid capsules comprising a compound A, dispersed in a composition C4.
    Type: Grant
    Filed: January 27, 2016
    Date of Patent: September 29, 2020
    Assignee: CALYXIA
    Inventors: Jamie Dean Walters, Jérôme Bibette
  • Patent number: 10767211
    Abstract: This invention provides an amadoriase having high reactivity with ?-fructosyl hexapeptide (?F6P) derived from the ?-chain amino terminus of glycated hemoglobin (HbAlc), which enables satisfactory quantification of ?F6P cleaved from HbAlc. A novel amadoriase is derived from the amadoriase of the genus Coniochaeta by substitution of one or more amino acids at positions selected from the group consisting of positions 62, 63, 102, 106, 110, 113, 355, and 419. Such amadoriase enables rapid, simple, accurate, and satisfactory quantification of ?F6P cleaved from HbAlc. With the use of such amadoriase, a method for measurement of HbAlc by an enzymatic method and a kit for measurement of HbAlc that are based on the same principle as used in the conventional standard method and excellent in terms of the relevance with the standard method.
    Type: Grant
    Filed: April 26, 2013
    Date of Patent: September 8, 2020
    Assignee: Kikkoman Corporation
    Inventors: Atsushi Ichiyanagi, Yosuke Masakari
  • Patent number: 10765661
    Abstract: The invention provides a method for improving growth performance animals. In particularly, the inventors of the invention found that the addition of a proteolytic enzyme (protease) to regular animal diets results in a significant improvement of growth performance if the proteolytic enzyme is supplemented and combined with vitamin C.
    Type: Grant
    Filed: February 10, 2016
    Date of Patent: September 8, 2020
    Assignee: DSM IP ASSETS B.V.
    Inventors: Aaron Cowieson, Inge Knap
  • Patent number: 10767204
    Abstract: The present disclosure provides methods and systems for generating sugars from biomass. The methods and systems provide increased yields of carbohydrates and fermentable sugars by combining enzyme recycling with removal of saccharification enzyme inhibitors and increased solids loading.
    Type: Grant
    Filed: December 20, 2013
    Date of Patent: September 8, 2020
    Assignee: Edeniq, Inc.
    Inventors: James Kacmar, Rich Kleinke, Bernard Cooker, Chris Kalinowski, Jon Van Volkinburg
  • Patent number: 10767206
    Abstract: The invention provides a new fermentation process for producing gellan gum, which can control the feeding amount and feeding speed of the nitrogen sources accurately and quantitatively, and control the growth and gum production of the strains, so as to significantly improve the controllability, stability and yield of the fermentation process for producing gellan gum.
    Type: Grant
    Filed: June 19, 2018
    Date of Patent: September 8, 2020
    Assignee: DSM IP ASSETS B.V.
    Inventors: Jie Zhao, Zhihui Gao, Wouter Adrianus Van Winden, Rogier Meulenberg
  • Patent number: 10767226
    Abstract: A honeycomb tube with a planar frame defining a fluidic path between a first planar surface and a second planar surface. A fluidic interface is located at one end of the planar frame. The fluidic interface has a fluidic inlet and fluidic outlet. The fluidic path further includes a well chamber having an well-substrate with a plurality of wells. The well chamber is arranged in the planar frame between the first or second surface and the well-substrate.
    Type: Grant
    Filed: December 21, 2018
    Date of Patent: September 8, 2020
    Assignee: Cepheid
    Inventors: Yuh-Min Chiang, Douglas B Dority, Dustin Dickens, Jennifer Glass, Reuel Van Atta
  • Patent number: 10760074
    Abstract: A composition for use in amplifying cDNA synthesized by a reverse transcription reaction and detecting RNA that serves as a template of the reverse transcription reaction, the composition containing a thermostable DNA polymerase, a thermostable ribonuclease H, and an intercalating dye. Since the composition of the present invention can suppress the influences to the nucleic acid amplification reaction by RNA that serves as a template for cDNA synthesis, the composition is useful in the detection of RNA, and more useful in quantification of RNA having a desired sequence by real-time RT-PCR.
    Type: Grant
    Filed: August 31, 2009
    Date of Patent: September 1, 2020
    Assignee: TAKARA BIO INC.
    Inventors: Kanako Usui, Takashi Uemori, Hiroyuki Mukai, Ikunoshin Kato
  • Patent number: 10760126
    Abstract: The present invention provides methods and kits for detecting RNA-interacting regions in genomic DNA.
    Type: Grant
    Filed: February 13, 2016
    Date of Patent: September 1, 2020
    Assignee: Bio-Rad Laboratories, Inc.
    Inventor: Steven Okino
  • Patent number: 10745674
    Abstract: The present invention relates to methods for producing polyketide synthase variants, and for altering the activity and/or substrate specificity of putative native and variant polyketide synthases. The present invention further relates to compositions comprising said polyketide synthase variants, compounds prepared using said polyketide synthase variants, and uses of said polyketide synthase variants. In one embodiment, said polyketide synthase variant is 2-pyrone synthase.
    Type: Grant
    Filed: March 5, 2015
    Date of Patent: August 18, 2020
    Assignees: SALK INSTITUTE FOR BIOLOGICAL STUDIES, THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
    Inventors: Joseph Noel, Kate Woods, Marianne Bowman, Gordon Louie, Nancy Anderson Da Silva, Javier Pascual Cardenas
  • Patent number: 10738316
    Abstract: A method for obtaining at least one microbial secondary metabolite or a derivative thereof, the method includes the step of heterologous expression of at least one synthetase of the secondary metabolite in at least one filamentous fungus. Also disclosed is an expression cassette, a plasmid vector including the expression cassette, an expression host, cyclodepsipeptides and a chimeric cyclodepsipeptide synthetase.
    Type: Grant
    Filed: March 20, 2015
    Date of Patent: August 11, 2020
    Assignee: TECHNISCHE UNIVERSITÄT BERLIN
    Inventors: Simon Boecker, Dirk Storm, Vera Meyer, Lennart Richter, Sophia Zobel, Franziska Wanka, Roderich Suessmuth, Agnes Muehlenweg
  • Patent number: 10738059
    Abstract: Biologically active compounds that can be used as photosensitizers for diagnostic and therapeutic applications, particularly for PDT of cancer, infections and other hyperproliferative diseases, fluorescence diagnosis and PDT treatment of a non-tumorous indication such as arthritis, inflammatory diseases, viral or bacterial infections, dermatological, ophthalmological or urological disorders are provided as well as providing methods to obtain them in pharmaceutical quality. In addition, conjugates are provided in which these photosensitizers are attached to water-soluble polymers via cleavable linkers that can be cleaved in the body under specific conditions. Another embodiment consists of formulating the desired tetrapyrrole photosensitizer into a pharmaceutical formulation to be injected into the body avoiding undesirable effects like solubility problems or delayed pharmacokinetics of the tetrapyrrole systems.
    Type: Grant
    Filed: February 27, 2017
    Date of Patent: August 11, 2020
    Assignee: Biolitec Unternehmensbeteiligungs II AG
    Inventors: Michael Staegemann, Rainer Haag, Arno Wiehe, Susanna Graefe, Burkhard Gitter, Volker Albrecht
  • Patent number: 10730909
    Abstract: A method for expressing, as a soluble protein or an active-form mutant enzyme, an enzyme that cannot be expressed as a soluble protein or an active-form enzyme in a heterologous expression system or that is obtained in a minute amount even when an active-form enzyme is expressed, the method including a technique for selecting an effective mutation site and a mutated amino acid. A new active-form mutant enzyme is also disclosed. The method involves: specifying an insoluble protein or an inactive-form enzyme; specifying a hydrophilic amino acid in a hydrophobic domain and/or a hydrophobic amino acid in a hydrophilic domain of an ?-helix structure portion of the insoluble protein or the inactive-form enzyme and preparing a gene that codes for an amino acid sequence in which a substitution is made to the hydrophilic amino acid in the hydrophobic domain and/or the hydrophobic amino acid in the hydrophilic domain.
    Type: Grant
    Filed: June 10, 2016
    Date of Patent: August 4, 2020
    Assignee: Toyama Prefectual University
    Inventors: Yasuhisa Asano, Daisuke Matsui, Yuko Oku
  • Patent number: 10722525
    Abstract: The present invention provides pharmacological compounds including an effector moiety conjugated to a binding moiety that directs the effector moiety to a biological target of interest. Likewise, the present invention provides compositions, kits, and methods (e.g., therapeutic, diagnostic, and imaging) including the compounds. The compounds can be described as a protein interacting binding moiety-drug conjugate (SDC-TRAP) compounds, which include a protein interacting binding moiety and an effector moiety. For example, in certain embodiments directed to treating cancer, the SDC-TRAP can include an Hsp90 inhibitor conjugated to a cytotoxic agent as the effector moiety.
    Type: Grant
    Filed: January 19, 2017
    Date of Patent: July 28, 2020
    Assignee: MADRIGAL PHARMACEUTICALS, INC.
    Inventors: Dinesh U. Chimmanamada, Weiwen Ying
  • Patent number: 10676751
    Abstract: Disclosed herein are materials useful for degrading plant biomass material. In exemplary embodiments, the plant material comprises one or more enzymes that are expressed in plants and/or bacteria. Specifically exemplified herein are plant degrading enzymes expressed in chloroplasts. The chloroplast expressed enzymes may be provided as cocktails for use in conjunction with conventional methods of converting biomass into biofuels, such as cellulosic ethanol.
    Type: Grant
    Filed: March 2, 2009
    Date of Patent: June 9, 2020
    Assignee: The Trustees of the University of Pennsylvania
    Inventor: Henry Daniell
  • Patent number: 10670607
    Abstract: This invention relates to bioassay method wherein the presence of a specific soluble antibody in a sample of a bodily fluid, plasma or serum, of an animal, including human, is qualitatively and/or quantitatively determined. The method employs, a first group labelled with an energy donor and a second group labelled with an energy acceptor; the first group, or second group, comprising an antigen of the soluble antibody and the second group, or first group, respectively, comprising a Fab binding moiety capable of binding to a Fab region of antibodies of said animal. The donor and the acceptor form an energy transfer pair capable of energy transfer. The invention further comprises a kit for the bioassay method.
    Type: Grant
    Filed: February 27, 2015
    Date of Patent: June 2, 2020
    Assignee: University of Helsinki
    Inventors: Klaus Hedman, Jussi Hepojoki, Satu Hepojoki, Antti Vaheri, Olli Vapalahti
  • Patent number: 10669578
    Abstract: The invention relates to an improved method for characterising a template polynucleotide. The method involves using a polymerase to prepare a modified polynucleotide which makes it easier to characterise than the template polynucleotide.
    Type: Grant
    Filed: February 19, 2015
    Date of Patent: June 2, 2020
    Assignee: Oxford Nanopore Technologies Ltd.
    Inventors: James Anthony Clarke, Marion Louise Crawford, James White
  • Patent number: 10626472
    Abstract: The invention relates to the use of a polysaccharide having at least four saccharide units, such as stachyose, as a glass-forming agent for the freeze-drying of a reaction mixture comprising an enzyme. In particular, the enzyme is a polymerase useful in a nucleic acid amplification reaction such as a Polymerase Chain Reaction. Compositions comprising such polysaccharides as well as methods for preparing them, kits containing them and methods for using them form further aspects of the invention.
    Type: Grant
    Filed: January 28, 2014
    Date of Patent: April 21, 2020
    Assignee: Fluorogenics Ltd
    Inventors: Martin Lee, Diane Lee, Mark Laverick
  • Patent number: 10626383
    Abstract: This disclosure relates to thermophilic family B DNA polymerases comprising a neutral amino acid residue at a certain position near the C-terminus of the catalytic domain, which corresponds to a position occupied by a basic amino acid residue in wild-type Pfu polymerase. Related uses, methods, and compositions are also provided. In some embodiments, the polymerases comprise a 3?-5? exonuclease domain and/or a sequence non-specific dsDNA binding domain.
    Type: Grant
    Filed: January 13, 2017
    Date of Patent: April 21, 2020
    Assignee: THERMO FISHER SCIENTIFIC BALTICS UAB
    Inventors: Remigijus Skirgaila, Renata Rimseliene, Rasa Sukackaite, Martyna Simutyte, Skaiste Valinskyte, Karolis Matjosaitis, Mindaugas Ukanis
  • Patent number: 10626386
    Abstract: The present invention relates to isolated polypeptides having cellulolytic enhancing activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.
    Type: Grant
    Filed: February 12, 2019
    Date of Patent: April 21, 2020
    Assignee: Novozymes, Inc.
    Inventors: Suchindra Maiyuran, Randall Kramer, Paul Harris
  • Patent number: 10597430
    Abstract: The present invention relates to a chimeric nucleotide sequence encoding peptides with antimicrobial activity, to be expressed on plants as bioreactors, plant cell or transformed plant material and E. coli, where plants and bacteria were used for scale-up production of antimicrobial peptide and that produces the peptide sequence derived from nucleotide sequence SEQ ID No. 1, SEQ ID No. 6 and SEQ ID No. 12. It includes a method for obtaining the amino acids sequence SEQ ID No. 9 derived from a chimeric nucleotide sequence which encodes to antimicrobial peptides, wherein said amino acids sequence is derived from the synthetic DNA sequence SEQ ID No. 1, obtained from a back-translation of the amino acid sequence of the peptide Ap-S of Argopecten purpuratus scallop.
    Type: Grant
    Filed: May 25, 2016
    Date of Patent: March 24, 2020
    Assignee: Instituto de Investigaciones Agropecuarias
    Inventors: Eduardo Andrés Tapia Rodríguez, Christian Fernando Montes Serey, Humberto Godofredo Prieto Encalada, Fabiola Alejandra Altamira Passalacqua, Gloria María Arenas Díaz
  • Patent number: 10577581
    Abstract: The present invention relates to the enzymatic synthesis of oligosaccharides, particularly, sialylated oligosaccharides comprising the carbohydrate moieties of the gangliosides GM3, GD3, and G.
    Type: Grant
    Filed: May 31, 2017
    Date of Patent: March 3, 2020
    Assignee: CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE (CNRS)
    Inventor: Eric Samain
  • Patent number: 10575485
    Abstract: The present invention is directed to plants having increased resistance to an imidazolinone herbicide. More particularly, the present invention includes wheat plants and triticale plants containing at least one IMI nucleic acid such as an imidazolinone resistant Brookton BR-8 or Krichauff K-42 cultivar. The present invention also includes seeds produced by these wheat plants and triticale plants and methods of controlling weeds in the vicinity of these wheat plants.
    Type: Grant
    Filed: June 25, 2015
    Date of Patent: March 3, 2020
    Assignee: Western Australian Agriculture Authority
    Inventors: David Bowran, Iain Barclay, Kevin F. Jose
  • Patent number: 10570467
    Abstract: The present disclosure provides genetically engineered microorganisms for tagatose production comprising one or more heterologous polynucleotides encoding polypeptides selected from cellodextrin transporter (Cdt-1), intracellular ?-glucosidase (Gh1-1), xylose reductase (XR), galactitol 2-dehydrogenase (Gdh) and an AraA polypeptide, wherein any biological activity of endogenous Gal1 is attenuated or eliminated. Also provided are genetically engineered microorganism for psicose production comprising one or more heterologous polynucleotides encoding polypeptides selected from alpha-glucoside permease (Agt1) and psicose epimerase (Dpe), wherein any biological activity of endogenous sucrose invertase (Suc2), hexose kinase 1 (Hxk1), hexose kinase 2 (Hxk2), or combinations thereof are attenuated or eliminated. Methods of converting lactose to tagatose and sucrose to psicose is also provided.
    Type: Grant
    Filed: September 27, 2017
    Date of Patent: February 25, 2020
    Assignees: THE BOARD OF TRUSTEES OF THE UNIVERSITY OF ILLINOIS, ZIMITECH
    Inventors: Yong-Su Jin, Jingjing Liu, Kulika Chomvong, Jamie H. D. Cate, Guochang Zhang
  • Patent number: 10568902
    Abstract: A modulated guanidine substituted polymer or nanoparticle has a guanidine moiety or on a plurality of repeating units of a polymer or on the surface of a nanoparticle where the guanidine moiety is modulated as a substituted amidinourea or amidinocarbamate or salt thereof. The modulated guanidine substituted polymer or nanoparticle can be prepared by direct amination of a N-Boc protected guanidine substituted conjugated polymer or N-Boc protected guanidine substituted nanoparticle, where an amine or alcohol is combined in solution or suspension with the protected conjugated polymer or nanoparticle and the resulting mixture is heated. The modulated guanidine substituted polymer or nanoparticle can be used in a cancer treatment formulation.
    Type: Grant
    Filed: December 14, 2018
    Date of Patent: February 25, 2020
    Assignee: THE FLORIDA INTERNATIONAL UNIVERSITY BOARD OF TRUSTEES
    Inventors: Joong Ho Moon, Md Salauddin Ahmed
  • Patent number: 10563186
    Abstract: The present invention relates to variants (mutants) of polypeptides, in particular Termamyl-like alpha-amylases, which variant has alpha-amylase activity and exhibits an alteration in at least one of the following properties relative to said parent alpha-amylase: substrate specificity, substrate binding, substrate cleavage pattern, thermal stability, pH/activity profile, pH/stability profile, stability towards oxidation, Ca2+ dependency, specific activity, in particular laundry and dish-wash applications.
    Type: Grant
    Filed: November 3, 2017
    Date of Patent: February 18, 2020
    Assignee: NOVOZYMES A/S
    Inventors: Carsten Andersen, Henrik Oestdal, Jan Peter Skagerlind
  • Patent number: 10550411
    Abstract: The invention provides a non-naturally occurring microbial organism having an acetyl-CoA pathway and the capability of utilizing syngas or syngas and methanol. In one embodiment, the invention provides a non-naturally occurring microorganism, comprising one or more exogenous proteins conferring to the microorganism a pathway to convert CO, CO2 and/or H2 to acetyl-coenzyme A (acetyl-CoA), methyl tetrahydrofolate (methyl-THF) or other desired products, wherein the microorganism lacks the ability to convert CO or CO2 and H2 to acetyl-CoA or methyl-THF in the absence of the one or more exogenous proteins. For example, the microbial organism can contain at least one exogenous nucleic acid encoding an enzyme or protein in an acetyl-CoA pathway. The microbial organism is capable of utilizing synthesis gases comprising CO, CO2 and/or H2, alone or in combination with methanol, to produce acetyl-CoA.
    Type: Grant
    Filed: February 6, 2018
    Date of Patent: February 4, 2020
    Assignee: Genomatica, Inc.
    Inventors: Mark J. Burk, Christophe H. Schilling, Anthony P. Burgard, John D. Trawick
  • Patent number: 10507233
    Abstract: Methods of capturing N-glycan linked glycomolecules including N-glycans, N-glycopeptides and N-glycoproteins are described. The methods provide substantially unbiased capture of charged and uncharged N-glycans and/or N-glycan linked glycomoleules. Binding reagents for substantially unbiased binding of N-glycans and/or N-glycan linked glycomolecules are also described.
    Type: Grant
    Filed: August 20, 2018
    Date of Patent: December 17, 2019
    Assignee: New England Biolabs, Inc.
    Inventors: Minyong Chen, Xiaofeng Shi, James C. Samuelson, Christopher H. Taron
  • Patent number: 10495653
    Abstract: Provided herein are assay control materials comprising stable analytes and lyophilized unstable analytes, and methods of making and using the same.
    Type: Grant
    Filed: January 20, 2017
    Date of Patent: December 3, 2019
    Assignee: Bio-Rad Laboratories, Inc.
    Inventors: Alireza Ebrahim, Karl De Vore, Christopher Spates
  • Patent number: 10485238
    Abstract: The present invention discloses Hemipteran insect inhibitory proteins, methods of using such proteins, nucleotide sequences encoding such proteins, methods of detecting and isolating such proteins, and their use in agricultural systems.
    Type: Grant
    Filed: December 4, 2018
    Date of Patent: November 26, 2019
    Assignee: Monsanto Technology LLC
    Inventors: James A. Baum, Artem G. Evdokimov, Farhad Moshiri, Timothy J. Rydel, Eric J. Sturman, Moritz von Rechenberg, Halong Vu, Andrew M. Wollacott, Meiying Zheng
  • Patent number: 10475953
    Abstract: A passage (3) for a sample solution is formed in a sapphire base body (2) used as a substrate for semiconductor devices. An LED (4) and a photodiode (5) are formed on the base body (2) by a semiconductor manufacturing process so that they face each other across the passage (3). The LED (4) emits light into the base body (2). This light is transmitted through the sample solution in the passage (3), undergoing absorption according to the concentration and other properties of the solution. The transmitted light passes through the base body (2) and reaches the photodiode (5), producing a detection signal corresponding to the incident light amount. Since the light source and photodetector are integrated with the base body (2) serving as a flow cell, the present device is small and lightweight. Furthermore, no cumbersome task of aligning optical axes in the device-assembling process is needed.
    Type: Grant
    Filed: April 24, 2015
    Date of Patent: November 12, 2019
    Assignee: SHIMADZU CORPORATION
    Inventor: Yusuke Nagai
  • Patent number: 10471037
    Abstract: The present application in one aspect provide Fc-containing polypeptide conjugates comprising an Fc-containing polypeptide conjugated to a conjugate moiety, wherein the Fc-containing polypeptide comprises an N-glycosylated Fc region comprising an acceptor glutamine residue flanked by an N-glycosylation site and wherein the conjugate moiety is conjugated to the Fc-containing polypeptide via the acceptor glutamine residue. Also provided are methods of making such Fc-containing polypeptide conjugates by using a wildtype or engineered transglutaminases. Further provided are engineered transglutaminases specifically designed for carrying out such reactions.
    Type: Grant
    Filed: May 22, 2018
    Date of Patent: November 12, 2019
    Assignee: CSPC Dophen Corporation
    Inventors: Sean Hu, Lisha Allen
  • Patent number: 10472671
    Abstract: This application relates to methods for ligating oligonucleotides having complementarity to a target nucleic acid, and amplifying the ligated oligonucleotides, where ligation and amplification occur in the same reaction mixture.
    Type: Grant
    Filed: December 1, 2015
    Date of Patent: November 12, 2019
    Assignee: Life Technologies Corporation
    Inventors: Shiaw-Min Chen, Elana Swartzman, David Ruff, Mark Shannon, Julia Lu, Stephen Hendricks
  • Patent number: 10450591
    Abstract: The invention relates to an Amycolatopsis strain derived from the Amycolatopsis Zyl 926 strain having at least one additional copy of the FCS and ECH genes encoding feruloyl-CoA-synthetase and enoyl-CoA-hydratase/aldolase integrated at the integration site of the ?C31 phage. The invention also relates to the method for producing such strains, the integration cassette of the FCS and ECH genes, the use of said strains in a method for producing vanillin, and a method for producing vanillin.
    Type: Grant
    Filed: September 22, 2016
    Date of Patent: October 22, 2019
    Assignee: LESAFFRE ET COMPAGNIE
    Inventors: Mylène Darricau, Thomas Desfougeres, Jean-Luc Pernodet
  • Patent number: 10435695
    Abstract: Polynucleotides encoding fusion proteins comprising interferons and luciferases which have biotherapeutic, diagnostic, and quality control applications in biotechnological, medical, and veterinary fields.
    Type: Grant
    Filed: May 1, 2017
    Date of Patent: October 8, 2019
    Assignee: The Government of the United States of America, as represented by the Secretary of Homeland Security
    Inventors: Michael Puckette, Max V. Rasmussen
  • Patent number: 10435440
    Abstract: The present invention relates to a chimeric nucleotide sequence encoding peptides with antimicrobial activity, to be expressed on plants as bioreactors, plant cell or transformed plant material and E. coli, where plants and bacteria were used for scale-up production of antimicrobial peptide and that produces the peptide sequence derived from nucleotide sequence SEQ ID No. 1, SEQ ID No. 6 and SEQ ID No. 12. It includes a method for obtaining the amino acids sequence SEQ ID No. 9 derived from a chimeric nucleotide sequence which encodes to antimicrobial peptides, wherein said amino acids sequence is derived from the synthetic DNA sequence SEQ ID No. 1, obtained from a back-translation of the amino acid sequence of the peptide Ap-S of Argopecten purpuratus scallop.
    Type: Grant
    Filed: May 25, 2016
    Date of Patent: October 8, 2019
    Assignee: Instituto de Investigaciones Agropecuarias
    Inventors: Eduardo Andrés Tapia Rodríguez, Christian Fernando Montes Serey, Humberto Godofredo Prieto Encalada, Fabiola Alejandra Altamira Passalacqua, Gloria María Arenas Díaz
  • Patent number: 10435680
    Abstract: A thermostable cellobiohydrolase, having a cellulose-binding motif domain including (A1) a polypeptide including an amino acid sequence represented by SEQ ID NO: 1, (B1) a polypeptide including an amino acid sequence in which at least one amino acid has been deleted, substituted, or added in the amino acid sequence represented by SEQ ID NO: 1, and having a cellulose-binding function, or (C1) a polypeptide including an amino acid sequence having 70% or greater sequence identity with the amino acid sequence represented by SEQ ID NO: 1, and having a cellulose-binding function, and also having a cellobiohydrolase catalytic domain, wherein the thermostable cellobiohydrolase exhibits hydrolysis activity against a substrate of phosphoric acid swollen Avicel at least under conditions of 95° C. and pH 5.5.
    Type: Grant
    Filed: March 23, 2017
    Date of Patent: October 8, 2019
    Assignee: Honda Motor Co., Ltd.
    Inventors: Jiro Okuma, Yoshitsugu Hirose, Migiwa Suda, Asuka Yamaguchi, Yasuhiro Kondo, Masaru Sato, Daisuke Shibata
  • Patent number: 10427131
    Abstract: Guanidine-functionalized perlite particles are provided. Nonwoven articles are also provided, including a fibrous porous matrix and guanidine-functionalized perlite particles enmeshed in the fibrous porous matrix. Laminated articles are additionally provided, including a first substrate and a second substrate sealed to the first substrate along at least a portion of a perimeter of the first substrate. The laminated article further includes guanidine-functionalized perlite particles disposed between the first substrate and the second substrate. Methods of detecting microorganisms or target cellular analytes in a fluid sample using guanidine-functionalized particles or laminated articles are also provided.
    Type: Grant
    Filed: March 15, 2016
    Date of Patent: October 1, 2019
    Assignee: 3M Innovative Properties Company
    Inventors: Manjiri T. Kshirsagar, George W. Griesgraber
  • Patent number: 10413617
    Abstract: Methods and products for altering or promoting the development of heart tissue are disclosed. The methods include the use of nucleic acids of cardiogenic inducing factor for treating a subject having heart disease.
    Type: Grant
    Filed: November 6, 2014
    Date of Patent: September 17, 2019
    Assignee: The Texas A&M University System
    Inventors: Larry F. Lemanski, Ashley Arms, Andrei Kochegarov
  • Patent number: 10406250
    Abstract: We have developed a model system of HIV reservoirs in neural cells by generating chimeric phosphorothioate-modified oligodeoxynucleotides (sODN) that specifically interact with neural cell DNA or RNA, and that further comprise a sequence of the HIV genome. In particular, we have conjugated the chimera sODN to a delivery vehicle (e.g. a superparamagnetic iron oxide nanoparticle (SPIO)) and have demonstrated specific delivery to neural cells, in vitro and in vivo. These model systems can be used to screen for agents that specifically target latent viral infection. In particular, using the model system, we have developed suicide MRI contrast agents that can be used to reduce the number of neural cells which harbor the virus, also provided herein. Our model system is translatable to other latent viruses as well.
    Type: Grant
    Filed: November 14, 2014
    Date of Patent: September 10, 2019
    Assignee: The General Hospital Corporation
    Inventor: Philip K. Liu
  • Patent number: 10398111
    Abstract: A novel maize variety designated PH41YP and seed, plants and plant parts thereof are provided. Methods for producing a maize plant comprise crossing maize variety PH41YP with another maize plant are provided. Methods for producing a maize plant containing in its genetic material one or more traits introgressed into PH41YP through backcross conversion and/or transformation, and to the maize seed, plant and plant part produced thereby are provided. Hybrid maize seed, plants or plant parts are produced by crossing the variety PH41YP or a locus conversion of PH41YP with another maize variety.
    Type: Grant
    Filed: March 19, 2018
    Date of Patent: September 3, 2019
    Assignee: PIONEER HI-BRED INTERNATIONAL, INC.
    Inventor: Susanne Groh
  • Patent number: 10392605
    Abstract: Provided are fusion proteins between p97 (melanotransferrin) and iduronate-2-sulfatase (IDS), and related compositions and methods of use thereof, for instance, to facilitate delivery of IDS across the blood-brain barrier (BBB) and/or improve its tissue penetration in CNS and/or peripheral tissues, and thereby treat and/or diagnose Hunter Syndrome (Mucopolysaccharidosis type II; MPS II) and related lysosomal storage disorders, including those having a central nervous system (CNS) component.
    Type: Grant
    Filed: February 12, 2015
    Date of Patent: August 27, 2019
    Assignee: Bioasis Technologies Inc.
    Inventors: Timothy Z. Vitalis, Reinhard Gabathuler
  • Patent number: 10392639
    Abstract: The present invention provides a process for the treatment of a composition comprising yeast cell walls comprising ?-1,3-glucans which are insoluble when extracted with water and partially soluble when extracted with DMSO, the process comprising contacting said composition with laminaripentaose-producing-?-1,3-glucanase and inactivating the laminaripentaose-producing-?-1,3-glucanase to result in a composition comprising yeast cell walls wherein the ?-1,3-glucans have an improved solubility in DMSO and the ratio of ?-glucans soluble in DMSO compared to water is greater than or equal to 2.
    Type: Grant
    Filed: March 20, 2015
    Date of Patent: August 27, 2019
    Assignee: DSM IP Assets B.V.
    Inventors: Ellen Speetjens, Peter Philip Lankhorst
  • Patent number: 10385344
    Abstract: The invention provides non-naturally occurring microbial organisms having a (2-hydroxy-3-methyl-4-oxobutoxy)phosphonate pathway, p-toluate pathway, and/or terephthalate pathway. The invention additionally provides methods of using such organisms to produce (2-hydroxy-3-methyl-4-oxobutoxy)phosphonate pathway, p-toluate pathway or terephthalate pathway.
    Type: Grant
    Filed: February 5, 2016
    Date of Patent: August 20, 2019
    Assignee: Genomatica, Inc.
    Inventor: Robin E. Osterhout
  • Patent number: 10385323
    Abstract: Presented herein are transposase enzymes and reaction conditions for improved fragmentation and tagging of nucleic acid samples, in particular altered transposases and reaction conditions which exhibit improved insertion sequence bias, as well as methods and kits using the same.
    Type: Grant
    Filed: June 28, 2018
    Date of Patent: August 20, 2019
    Assignee: ILLUMINA, INC.
    Inventors: Christian Gloeckner, Amirali Kia, Erin Bomati, Molly He, Haiying Li Grunenwald, Scott Kuersten, Trina Faye Osothprarop, Darin Haskins, Joshua Burgess, Anupama Khanna, Daniel Schlingman, Ramesh Vaidyanathan
  • Patent number: 10373703
    Abstract: The present invention provides a method of a method of designing an implementation of a DNA assembly. In an exemplary embodiment, the method includes (1) receiving a list of DNA sequence fragments to be assembled together and an order in which to assemble the DNA sequence fragments, (2) designing DNA oligonucleotides (oligos) for each of the DNA sequence fragments, and (3) creating a plan for adding flanking homology sequences to each of the DNA oligos. In an exemplary embodiment, the method includes (1) receiving a list of DNA sequence fragments to be assembled together and an order in which to assemble the DNA sequence fragments, (2) designing DNA oligonucleotides (oligos) for each of the DNA sequence fragments, and (3) creating a plan for adding optimized overhang sequences to each of the DNA oligos.
    Type: Grant
    Filed: May 5, 2016
    Date of Patent: August 6, 2019
    Assignee: The Regents of the University of California
    Inventor: Nathan J. Hillson