Enzyme (e.g., Ligases (6. ), Etc.), Proenzyme; Compositions Thereof; Process For Preparing, Activating, Inhibiting, Separating, Or Purifying Enzymes Patents (Class 435/183)
  • Patent number: 11198885
    Abstract: An expression increasing element comprising a 36 nucleotide DNA sequence that can be used to increase the expression of genes, and in particular to increase the expression of endogenous plant genes, in plants is disclosed. Also disclosed are plants, plant parts, and commodity plant products comprising the expression increasing element along with related methods of using the expression increasing element and plants comprising the expression increasing element.
    Type: Grant
    Filed: March 2, 2021
    Date of Patent: December 14, 2021
    Assignee: Inari Agriculture Technology, Inc.
    Inventor: Hannes Bart Claeys
  • Patent number: 11174297
    Abstract: An object of the present invention is to identify an enzyme having activity of synthesizing dihydrotentoxin that is a tentoxin precursor and an enzyme having activity of synthesizing tentoxin using dihydrotentoxin as a substrate. The present invention concerns a tentoxin synthesis-related gene encoding a protein comprising the amino acid sequence of SEQ ID NO: 16 and having activity of nonribosomal peptide synthesis of dihydrotentoxin and a tentoxin synthesis-related gene encoding a protein comprising the amino acid sequence of SEQ ID NO: 18 and having activity of converting dihydrotentoxin to tentoxin.
    Type: Grant
    Filed: March 16, 2017
    Date of Patent: November 16, 2021
    Assignee: KUMIAI CHEMICAL INDUSTRY CO., LTD.
    Inventors: Takashi Kubo, Masayuki Machida, Tomonori Fujioka, Shigenari Yamaguchi, Kiyoshi Kawai
  • Patent number: 11172657
    Abstract: Provided is a method for developing a secondary organ by using a non-human animal in which organ formation is inhibited, for the purpose of establishing a process for producing a functional cell such as a ? cell within the body of an animal such as a pig, the method including the step of raising a newborn or a fetus of the non-human animal in which organ formation is inhibited by complementing at least a part of the function of the organ whose formation is inhibited.
    Type: Grant
    Filed: September 18, 2018
    Date of Patent: November 16, 2021
    Assignee: PORMEDTEC CO., LTD.
    Inventors: Hiroshi Nagashima, Hitomi Matsunari, Kazuaki Nakano, Koki Hasegawa
  • Patent number: 11174487
    Abstract: Methods of constructing a cell comprising in its chromosome one or more copies of an open reading frame (ORF) or operon encoding at least one polypeptide of interest, each copy being under the transcriptional control of a heterologous promoter using a site specific recombinase and in vivo integration by recombination; resulting cells, and methods for producing a polypeptide of interest using the resulting cells.
    Type: Grant
    Filed: April 7, 2017
    Date of Patent: November 16, 2021
    Assignee: Novozymes A/S
    Inventors: Anne Breuner, Michael Dolberg Rasmussen
  • Patent number: 11155801
    Abstract: Unglycosylated lysostaphin variant protein, nucleic acid molecule, vector and host cell, as well as a method for production of unglycosylated lysostaphin variant protein in a yeast expression system are provided. The proteins are produced in a Pichia pastoris expression system and have been shown to have activity equivalent to wild-type lysostaphin. The lysostaphin variant proteins can be used as therapeutic proteins for treatment of diseases such as Staphylococcus aureus infection.
    Type: Grant
    Filed: July 16, 2014
    Date of Patent: October 26, 2021
    Assignee: Trustees of Dartmouth College
    Inventors: Karl E. Griswold, Hongliang Zhao
  • Patent number: 11149293
    Abstract: This invention provides a fermentation method of producing gellan gum, wherein a certain amount of fermentation broth is retained in the fermentor as a seed for next batch fermentation or transferred to another fermentor as a seed for next batch fermentation in that fermentor. The fermentation method of this invention reduces fermentation cost of gellan gum and lowers contamination risk during seed cultivation.
    Type: Grant
    Filed: November 1, 2018
    Date of Patent: October 19, 2021
    Assignees: Zhejiang DSM Zhongken Biotechnology Co. Ltd, DSM IP Assets B.V.
    Inventors: Jie Zhao, Wouter Adrianus Van Winden, Rogier Meulenberg, Zhihui Gao
  • Patent number: 11130946
    Abstract: The present invention relates to variants of a parent glucoamylase having altered properties (e.g., improved thermostability and/or specific activity). In particular, the present invention provides compositions comprising the variant glucoamylases, including starch hydrolyzing compositions, animal feed compositions and cleaning compositions. The invention also relates to DNA constructs encoding the variants and methods of producing the glucoamylase variants in host cells.
    Type: Grant
    Filed: January 14, 2020
    Date of Patent: September 28, 2021
    Assignee: Danisco US Inc.
    Inventors: Pieter Van Solingen, Richard R. Bott, Wolfgang Aehle, Martijn Scheffers
  • Patent number: 11091785
    Abstract: The present invention relates to enzyme compositions for high temperature saccharification of cellulosic material and to uses thereof.
    Type: Grant
    Filed: April 3, 2020
    Date of Patent: August 17, 2021
    Assignee: Novoyzmes, Inc.
    Inventors: Brett McBrayer, Tarana Shaghasi, Elena Vlasenko
  • Patent number: 11085048
    Abstract: The present invention provides an expression cassette containing a polynucleotide coding sequence for a hydroxycinnamoyl-CoA hydratase-lyase (HCHL), which is operably linked to a heterologous promoter. Also provided are methods of engineering plants that have reduced lignification, as well as cells, plant parts, and plant tissues from such engineered plants.
    Type: Grant
    Filed: April 26, 2019
    Date of Patent: August 10, 2021
    Assignee: The Regents of the University of California
    Inventors: Dominique Loqué, Aymerick Guillaume Eudes
  • Patent number: 11073518
    Abstract: The present invention relates to a prefabricated microparticle for performing detection, preferably a digital detection and/or quantitation of an analyte. Furthermore, it also relates to a detection and/or quantitation of multiple analytes by prefabricated microparticles. It also relates to a collection of such prefabricated microparticles and to the use of such microparticle(s) and/or of such collection. Furthermore, the present invention also relates to a method of performing a detection and/or quantitation of an analyte in a sample wherein a microparticle or collection of microparticles are used. In one embodiment, in the collection of microparticles, individual microparticles are tailored for the detection of specific analytes and can be distinguished from each other by a specific label indicating the respective analyte for which the individual microparticle is specific.
    Type: Grant
    Filed: December 22, 2017
    Date of Patent: July 27, 2021
    Assignee: BLINK AG
    Inventors: Katrin Steinmetzer, Stephan Hubold, Thomas Ellinger, Eugen Ermantraut, Torsten Schulz
  • Patent number: 11072783
    Abstract: An isolated and/or purified ?-glucanotransferase from Exiguobacterium Acetylicum, recombinantly engineered variants thereof, active fragments thereof, synthetic nucleic acids encoding the ?-glucanotransferase and variants thereof, host cells comprising the synthetic nucleic acids, and compositions comprising the ?-glucanotransferase are provided. Methods of using the compositions include the manufacture of oligosaccharides.
    Type: Grant
    Filed: October 6, 2016
    Date of Patent: July 27, 2021
    Assignee: DUPONT NUTRITION BIOSCIENCES APS
    Inventor: Slavko Kralj
  • Patent number: 11066655
    Abstract: Cyanophage Syn5 RNA polymerase (RNAP) and methods of purifying it are provided. Cyanophage Syn5 RNAP having one or more promoter mutations are provided. Methods of expressing cyanophage Syn5 RNAP are provided. Novel promoter sequences and in vitro transcription systems utilizing Syn5 RNAP are provided. Methods of making high range ssRNA ladders are also provided. Methods of incorporating 2?-modified dNTPs using Syn5 RNA polymerase are provided.
    Type: Grant
    Filed: February 12, 2016
    Date of Patent: July 20, 2021
    Assignee: President and Fellows of Harvard College
    Inventors: Charles C. Richardson, Bin Zhu, Stanley Tabor
  • Patent number: 11034938
    Abstract: There is provided a microbial cell expressing a mutant AlkB enzyme, the mutant AlkB enzyme comprising at least one point mutation in the wild type sequence of AlkB, wherein the point mutation is at amino acid position V129 and/or T136 of the wild type AlkB enzyme. There is also provided a method for producing omega-hydroxy carboxylic acid and/or ester thereof using this cell.
    Type: Grant
    Filed: July 3, 2017
    Date of Patent: June 15, 2021
    Assignee: EVONIK OPERATIONS GMBH
    Inventors: Arne Skerra, Ludwig Kirmair, Steffen Schaffer, Christoph Schorsch, Mirja Wessel, Thomas Haas
  • Patent number: 11021726
    Abstract: The invention relates to a method for the production of a solid bio-product wherein at least 80% of the original indigestible oligosaccharide (raffmose, stachyose and verbascose) content has been degraded into digestible mono- and disaccharides, comprising the following steps: 1) providing a mixture of milled or flaked or otherwise disintegrated biomass, comprising oligosaccharides and optionally polysaccharides and further comprising proteinaceous plant parts, water and one or more enzyme preparations containing .alpha.-galactosidase(s); 2) reacting the mixture resulting from step (1) under continuous mixing and under conditions where the water content in the initial mixture does not exceed 65% by weight, for 0.15-36 hours at a temperature of about 20-65° C.; 3) incubating the reacted mixture from step (2) at a temperature and in a time period which inactivate said ?-galactosidase(s), as well as solid bio-products obtainable by such method.
    Type: Grant
    Filed: May 23, 2017
    Date of Patent: June 1, 2021
    Assignee: HAMLET PROTEIN A/S
    Inventors: Katrine Hvid Ellegard, Karl Kristian Thomsen, Ole Kaae Hansen
  • Patent number: 11021729
    Abstract: The present invention provides engineered ketoreductase and phosphite dehydrogenase enzymes having improved properties as compared to a naturally occurring wild-type ketoreductase and phosphite dehydrogenase enzymes, as well as polynucleotides encoding the engineered ketoreductase and phosphite dehydrogenase enzymes, host cells capable of expressing the engineered ketoreductase and phosphite dehydrogenase enzymes, and methods of using the engineered ketoreductase and phosphite dehydrogenase enzymes to synthesize a chiral catalyst used in the synthesis of antiviral compounds, such as nucleoside inhibitors. The present invention further provides methods of using the engineered enzymes to deracemize a chiral alcohol in a one-pot, multi-enzyme system.
    Type: Grant
    Filed: April 13, 2018
    Date of Patent: June 1, 2021
    Assignee: Codexis, Inc.
    Inventors: Jeffrey C. Moore, Jack Liang, Jonathan Penfield, Jovana Nazor, Nikki Dellas, Vesna Mitchell, Da Duan, Iman Farasat, Agustina Rodriguez-Granillo, Grant Murphy, Nicholas Marshall
  • Patent number: 11015149
    Abstract: Bioactive coatings that include a base and a protein associated with the base for actively promoting the removal of organic stains are provided. In aspects, bioactive coatings that are stabilized against inactivation by weathering are provided including a base associated with a chemically modified enzyme, and, optionally a first polyoxyethylene present in the base and independent of the enzyme. The coatings are optionally overlayered onto a substrate to form an active coating facilitating the removal of organic stains or organic material from food, insects, or the environment.
    Type: Grant
    Filed: November 13, 2017
    Date of Patent: May 25, 2021
    Assignees: Toyota Motor Corporation, Regents of the University of Minnesota
    Inventors: Andreas Buthe, Ping Wang, Songtao Wu, Hongfei Jia, Masahiko Ishii, Minjuan Zhang
  • Patent number: 10988738
    Abstract: The present invention provides a microorganism-derived soluble coenzyme-binding glucose dehydrogenase which catalyzes a reaction for oxidizing glucose in the presence of an electron acceptor, has an activity to maltose as low as 5% or less, and is inhibited by 1,10-phenanthroline. The invention also provides a method for producing the coenzyme-binding glucose dehydrogenase, and a method and a reagent for measuring employing the coenzyme-binding glucose dehydrogenase. According to the invention, the coenzyme-binding glucose dehydrogenase can be applied to an industrial field, and a use becomes possible also in a material production or analysis including a method for measuring or eliminating glucose in a sample using the coenzyme-binding glucose dehydrogenase as well as a method for producing an organic compound. It became also possible to provide a glucose sensor capable of accurately measuring a blood sugar level.
    Type: Grant
    Filed: February 15, 2019
    Date of Patent: April 27, 2021
    Assignees: Ikeda Food Research Co., Ltd., PHC Corporation
    Inventors: Hironori Omura, Hirokazu Sanada, Takako Yada, Tetsunari Morita, Mika Kuyama, Tokuji Ikeda, Kenji Kano, Seiya Tsujimura
  • Patent number: 10988714
    Abstract: Bioactive coatings that include a base and a protein associated with the base for actively promoting the removal of organic stains are provided. In aspects, bioactive coatings that are stabilized against inactivation by weathering are provided including a base associated with a chemically modified enzyme, and, optionally a first polyoxyethylene present in the base and independent of the enzyme. The coatings are optionally overlayered onto a substrate to form an active coating facilitating the removal of organic stains or organic material from food, insects, or the environment.
    Type: Grant
    Filed: November 13, 2017
    Date of Patent: April 27, 2021
    Assignees: Regents of the University of Minnesota, Toyota Motor Corporation
    Inventors: Andreas Buthe, Ping Wang, Songtao Wu, Hongfei Jia, Masahiko Ishii, Minjuan Zhang
  • Patent number: 10973887
    Abstract: Methods to introduce highly phosphorylated mannopyranosyl oligosaccharide derivatives containing mannose-6-phosphate (M6P), or other oligosaccharides bearing other terminal hexoses, to carbonyl groups on oxidized glycans of glycoproteins while retaining their biological activity are described. The methods are useful for modifying glycoproteins, including those produced by recombinant protein expression systems, to increase uptake by cell surface receptor-mediated mechanisms, thus improving their therapeutic efficacy in a variety of applications.
    Type: Grant
    Filed: June 12, 2019
    Date of Patent: April 13, 2021
    Assignee: GENZYME CORPORATION
    Inventor: Yunxiang Zhu
  • Patent number: 10941185
    Abstract: The present invention discloses a strain of bacteria producing DHA and/or EPA, six gene fragments in the bacterial genome, and uses thereof. The strain is Schizoochytrium limacinum HS01, which has the accession number of CGMCC No. 13746 at China General Microbiological Culture Collection Center. The six gene fragments are composed of gene fragment 1 to gene fragment 6, and the nucleotide sequences are sequentially as shown in SEQ ID NO: 3 to SEQ ID NO: 8 in the Sequence Listing. The experiments prove that fermentation broth containing DHA and EPA can be obtained by fermenting Schizoochytrium limacinum HS01; the recombinant strain is obtained by introducing gene fragment 1 to gene fragment 6 into Schizoochytrium limacinum MYA-1381; the ability the recombinant strain for producing DHA and EPA is greatly improved.
    Type: Grant
    Filed: November 26, 2019
    Date of Patent: March 9, 2021
    Assignee: XIAMEN HUISON BIOTECH CO., LTD.
    Inventors: Liyi Chen, Huichang Zhong, Shuirong Chen
  • Patent number: 10934324
    Abstract: A method of improving protein functionality includes obtaining a protein such as protein contained within exclusion bodies contained within bacteria or yeast used for the recombinant expression of the protein. The exclusion bodies are solubilized using a chaotropic agent such as guanidine or urea. The protein is then subject to denaturation conditions and optionally purified. A liquid containing the denatured protein is loaded into a vessel that is angled relative to horizontal. The vessel is then rotated in the angled configuration at a rate within that is less than 10,000 RPM for period of time. Refolded protein is formed by the high shear conditions formed in the thin film of fluid formed in the inner surface of the rotating vessel. Refolding can be performed in a batch mode or a continuous mode. The process may be scaled up for industrial applications by using multiple vessels.
    Type: Grant
    Filed: September 3, 2014
    Date of Patent: March 2, 2021
    Assignee: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
    Inventors: Gregory A. Weiss, Colin L. Raston, Tom Z. Yuan, Callum Ormonde, Stephan Timothy Kudlacek
  • Patent number: 10925293
    Abstract: The present invention relates to methods of producing lipids. In particular, the present invention relates to methods of increasing the level of one or more non-polar lipids and/or the total non-polar lipid content in a transgenic organism or part thereof. In one particular embodiment, the present invention relates to the use of an acyltransferase, for example, a monoacylglycerol acyltransferase (MGAT) to increase the level of one or more non-polar lipids and/or the total non-polar lipid content in plants, plant seed and/or leaves, algae and fungi.
    Type: Grant
    Filed: August 25, 2015
    Date of Patent: February 23, 2021
    Assignee: Commonwealth Scientific and Industrial Research Organisation
    Inventors: James Robertson Petrie, Thomas Vanhercke, Pushkar Shrestha, Qing Liu, Surinder Pal Singh
  • Patent number: 10900045
    Abstract: The present invention provides a novel fluorescent protein. A novel protein includes the following protein (F1): (F1) a protein consisting of an amino acid sequence of SEQ ID NO: 1, wherein in the amino acid sequence of SEQ ID NO: 1, a 52nd amino acid Xaa52 is an arbitrary amino acid, a 133rd amino acid Xaa133 is an arbitrary amino acid, and a 154th amino acid Xaa154 is an arbitrary amino acid.
    Type: Grant
    Filed: June 13, 2016
    Date of Patent: January 26, 2021
    Assignee: NEC Solution Innovators. Ltd.
    Inventors: Ikuo Shiratori, Akihisa Shimizu, Katsunori Horii, Hiroshi Mishima, Iwao Waga
  • Patent number: 10883069
    Abstract: Bioactive coatings that include a base and a protein associated with the base for actively promoting the removal of organic stains are provided. In aspects, bioactive coatings that are stabilized against inactivation by weathering are provided including a base associated with a chemically modified enzyme, and, optionally a first polyoxyethylene present in the base and independent of the enzyme. The coatings are optionally overlayered onto a substrate to form an active coating facilitating the removal of organic stains or organic material from food, insects, or the environment.
    Type: Grant
    Filed: November 13, 2017
    Date of Patent: January 5, 2021
    Assignees: Toyota Motor Corporation, Regents of the University of Minnesota
    Inventors: Andreas Buthe, Ping Wang, Songtao Wu, Hongfei Jia, Masahiko Ishii, Minjuan Zhang
  • Patent number: 10883094
    Abstract: Transposase polypeptides and polynucleotides are provided, which have a high activity in mammalian cells. Methods for engineering cells, such as chimeric antigen T-cells, with the transposes are also provided.
    Type: Grant
    Filed: January 14, 2020
    Date of Patent: January 5, 2021
    Assignee: BOARD OF REGENTS, THE UNIVERSITY OF TEXAS SYSTEM
    Inventors: Laurence J. N. Cooper, Natalya Belousova
  • Patent number: 10869494
    Abstract: Providing a D-psicose-containing sweetener with the modification of the taste of D-psicose, comprising D-psicose, a sugar alcohol and/or a high intensity sweetener, preferably containing D-psicose as the main component, particularly a low-calorie sweetener and/or a sweetener giving refreshing feel in the oral cavity, as well as foods and drinks obtained by using the D-psicose-containing sweetener with the modification of the taste of D-psicose, and other products given with sweetness. The sugar alcohol is one or more sugar alcohols selected from the group consisting of sorbitol, mannitol, lactitol, maltitol, xylitol and erythritol, while the high intensity sweetener is one or more high intensity sweeteners as selected from aspartame, acesulfame K, sodium cyclamate, sodium saccharin, Sucralose (under trade name), stevia sweetener, dulcin, taumatin, neotame and monellin.
    Type: Grant
    Filed: March 26, 2007
    Date of Patent: December 22, 2020
    Assignee: MATSUTANI CHEMICAL INDUSTRY CO., LTD.
    Inventors: Hideki Fujihara, Kazuhiro Okuma, Tetsuo Iida, Ken Izumori, Masaaki Tokuda, Kazuhiro Fukada
  • Patent number: 10829770
    Abstract: Polynucleotides encoding fusion proteins contain a secretable luciferase fused to a modified polypeptide of interest are disclosed. The polypeptide of interest has been modified to remove a native N-terminal secretion sequence and has been replaced by the secretable luciferase. One example of a modified polypeptide of interest is interferon. The polynucleotides and fusion proteins have biotherapeutic, diagnostic, and quality control applications in biotechnological, medical, and veterinary fields. Methods for producing the secretable fusion protein are also disclosed.
    Type: Grant
    Filed: September 16, 2019
    Date of Patent: November 10, 2020
    Assignee: The Government of the United States of America, as represented by the Secretary of Homeland Security
    Inventors: Michael Puckette, Max V. Rasmussen
  • Patent number: 10800848
    Abstract: Disclosed herein are compositions and pharmaceutical formulations that comprise a binding moiety conjugated to a polynucleic acid molecule and a polymer. Also described herein include methods for treating a cancer which utilize a composition or a pharmaceutical formulation comprising a binding moiety conjugated to a polynucleic acid molecule and a polymer.
    Type: Grant
    Filed: September 11, 2018
    Date of Patent: October 13, 2020
    Assignee: AVIDITY BIOSCIENCES, INC.
    Inventors: Andrew John Geall, Venkata Ramana Doppalapudi, David Sai-Ho Chu, Michael Caramian Cochran, Rachel Elizabeth Johns, Palani Balu, Rob Burke, Beatrice Diana Darimont
  • Patent number: 10792341
    Abstract: Methods of treating glycogen storage disease type II, by administering acid ?-glucosidase, are described, as are compositions for use in treatment of glycogen storage disease type II.
    Type: Grant
    Filed: January 19, 2018
    Date of Patent: October 6, 2020
    Assignee: Duke University
    Inventor: Yuan-Tsong Chen
  • Patent number: 10793922
    Abstract: The present invention provides methods for rapidly identifying an RNA viral infection using an isothermal nucleic acid amplification reaction that can be carried out extracted RNA in the context of a crude biological sample.
    Type: Grant
    Filed: October 20, 2015
    Date of Patent: October 6, 2020
    Assignee: ENVIROLOGIX INC.
    Inventors: Lars Peters, Stephen A. Judice, Daniel Shaffer, Breck Parker
  • Patent number: 10786798
    Abstract: The present invention relates to a method for preparing solid capsules comprising a compound A, dispersed in a composition C4.
    Type: Grant
    Filed: January 27, 2016
    Date of Patent: September 29, 2020
    Assignee: CALYXIA
    Inventors: Jamie Dean Walters, Jérôme Bibette
  • Patent number: 10787519
    Abstract: Disclosed herein are compositions and pharmaceutical formulations that comprise a binding moiety conjugated to a polynucleic acid molecule and a polymer. Also described herein include methods for treating a cancer which utilize a composition or a pharmaceutical formulation comprising a binding moiety conjugated to a polynucleic acid molecule and a polymer.
    Type: Grant
    Filed: September 11, 2018
    Date of Patent: September 29, 2020
    Assignee: AVIDITY BIOSCIENCES, INC.
    Inventors: Andrew John Geall, Venkata Ramana Doppalapudi, David Sai-Ho Chu, Michael Caramian Cochran, Rachel Elizabeth Johns, Palani Balu, Rob Burke, Beatrice Diana Darimont
  • Patent number: 10767206
    Abstract: The invention provides a new fermentation process for producing gellan gum, which can control the feeding amount and feeding speed of the nitrogen sources accurately and quantitatively, and control the growth and gum production of the strains, so as to significantly improve the controllability, stability and yield of the fermentation process for producing gellan gum.
    Type: Grant
    Filed: June 19, 2018
    Date of Patent: September 8, 2020
    Assignee: DSM IP ASSETS B.V.
    Inventors: Jie Zhao, Zhihui Gao, Wouter Adrianus Van Winden, Rogier Meulenberg
  • Patent number: 10765661
    Abstract: The invention provides a method for improving growth performance animals. In particularly, the inventors of the invention found that the addition of a proteolytic enzyme (protease) to regular animal diets results in a significant improvement of growth performance if the proteolytic enzyme is supplemented and combined with vitamin C.
    Type: Grant
    Filed: February 10, 2016
    Date of Patent: September 8, 2020
    Assignee: DSM IP ASSETS B.V.
    Inventors: Aaron Cowieson, Inge Knap
  • Patent number: 10767204
    Abstract: The present disclosure provides methods and systems for generating sugars from biomass. The methods and systems provide increased yields of carbohydrates and fermentable sugars by combining enzyme recycling with removal of saccharification enzyme inhibitors and increased solids loading.
    Type: Grant
    Filed: December 20, 2013
    Date of Patent: September 8, 2020
    Assignee: Edeniq, Inc.
    Inventors: James Kacmar, Rich Kleinke, Bernard Cooker, Chris Kalinowski, Jon Van Volkinburg
  • Patent number: 10767211
    Abstract: This invention provides an amadoriase having high reactivity with ?-fructosyl hexapeptide (?F6P) derived from the ?-chain amino terminus of glycated hemoglobin (HbAlc), which enables satisfactory quantification of ?F6P cleaved from HbAlc. A novel amadoriase is derived from the amadoriase of the genus Coniochaeta by substitution of one or more amino acids at positions selected from the group consisting of positions 62, 63, 102, 106, 110, 113, 355, and 419. Such amadoriase enables rapid, simple, accurate, and satisfactory quantification of ?F6P cleaved from HbAlc. With the use of such amadoriase, a method for measurement of HbAlc by an enzymatic method and a kit for measurement of HbAlc that are based on the same principle as used in the conventional standard method and excellent in terms of the relevance with the standard method.
    Type: Grant
    Filed: April 26, 2013
    Date of Patent: September 8, 2020
    Assignee: Kikkoman Corporation
    Inventors: Atsushi Ichiyanagi, Yosuke Masakari
  • Patent number: 10767226
    Abstract: A honeycomb tube with a planar frame defining a fluidic path between a first planar surface and a second planar surface. A fluidic interface is located at one end of the planar frame. The fluidic interface has a fluidic inlet and fluidic outlet. The fluidic path further includes a well chamber having an well-substrate with a plurality of wells. The well chamber is arranged in the planar frame between the first or second surface and the well-substrate.
    Type: Grant
    Filed: December 21, 2018
    Date of Patent: September 8, 2020
    Assignee: Cepheid
    Inventors: Yuh-Min Chiang, Douglas B Dority, Dustin Dickens, Jennifer Glass, Reuel Van Atta
  • Patent number: 10760126
    Abstract: The present invention provides methods and kits for detecting RNA-interacting regions in genomic DNA.
    Type: Grant
    Filed: February 13, 2016
    Date of Patent: September 1, 2020
    Assignee: Bio-Rad Laboratories, Inc.
    Inventor: Steven Okino
  • Patent number: 10760074
    Abstract: A composition for use in amplifying cDNA synthesized by a reverse transcription reaction and detecting RNA that serves as a template of the reverse transcription reaction, the composition containing a thermostable DNA polymerase, a thermostable ribonuclease H, and an intercalating dye. Since the composition of the present invention can suppress the influences to the nucleic acid amplification reaction by RNA that serves as a template for cDNA synthesis, the composition is useful in the detection of RNA, and more useful in quantification of RNA having a desired sequence by real-time RT-PCR.
    Type: Grant
    Filed: August 31, 2009
    Date of Patent: September 1, 2020
    Assignee: TAKARA BIO INC.
    Inventors: Kanako Usui, Takashi Uemori, Hiroyuki Mukai, Ikunoshin Kato
  • Patent number: 10745674
    Abstract: The present invention relates to methods for producing polyketide synthase variants, and for altering the activity and/or substrate specificity of putative native and variant polyketide synthases. The present invention further relates to compositions comprising said polyketide synthase variants, compounds prepared using said polyketide synthase variants, and uses of said polyketide synthase variants. In one embodiment, said polyketide synthase variant is 2-pyrone synthase.
    Type: Grant
    Filed: March 5, 2015
    Date of Patent: August 18, 2020
    Assignees: SALK INSTITUTE FOR BIOLOGICAL STUDIES, THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
    Inventors: Joseph Noel, Kate Woods, Marianne Bowman, Gordon Louie, Nancy Anderson Da Silva, Javier Pascual Cardenas
  • Patent number: 10738059
    Abstract: Biologically active compounds that can be used as photosensitizers for diagnostic and therapeutic applications, particularly for PDT of cancer, infections and other hyperproliferative diseases, fluorescence diagnosis and PDT treatment of a non-tumorous indication such as arthritis, inflammatory diseases, viral or bacterial infections, dermatological, ophthalmological or urological disorders are provided as well as providing methods to obtain them in pharmaceutical quality. In addition, conjugates are provided in which these photosensitizers are attached to water-soluble polymers via cleavable linkers that can be cleaved in the body under specific conditions. Another embodiment consists of formulating the desired tetrapyrrole photosensitizer into a pharmaceutical formulation to be injected into the body avoiding undesirable effects like solubility problems or delayed pharmacokinetics of the tetrapyrrole systems.
    Type: Grant
    Filed: February 27, 2017
    Date of Patent: August 11, 2020
    Assignee: Biolitec Unternehmensbeteiligungs II AG
    Inventors: Michael Staegemann, Rainer Haag, Arno Wiehe, Susanna Graefe, Burkhard Gitter, Volker Albrecht
  • Patent number: 10738316
    Abstract: A method for obtaining at least one microbial secondary metabolite or a derivative thereof, the method includes the step of heterologous expression of at least one synthetase of the secondary metabolite in at least one filamentous fungus. Also disclosed is an expression cassette, a plasmid vector including the expression cassette, an expression host, cyclodepsipeptides and a chimeric cyclodepsipeptide synthetase.
    Type: Grant
    Filed: March 20, 2015
    Date of Patent: August 11, 2020
    Assignee: TECHNISCHE UNIVERSITÄT BERLIN
    Inventors: Simon Boecker, Dirk Storm, Vera Meyer, Lennart Richter, Sophia Zobel, Franziska Wanka, Roderich Suessmuth, Agnes Muehlenweg
  • Patent number: 10730909
    Abstract: A method for expressing, as a soluble protein or an active-form mutant enzyme, an enzyme that cannot be expressed as a soluble protein or an active-form enzyme in a heterologous expression system or that is obtained in a minute amount even when an active-form enzyme is expressed, the method including a technique for selecting an effective mutation site and a mutated amino acid. A new active-form mutant enzyme is also disclosed. The method involves: specifying an insoluble protein or an inactive-form enzyme; specifying a hydrophilic amino acid in a hydrophobic domain and/or a hydrophobic amino acid in a hydrophilic domain of an ?-helix structure portion of the insoluble protein or the inactive-form enzyme and preparing a gene that codes for an amino acid sequence in which a substitution is made to the hydrophilic amino acid in the hydrophobic domain and/or the hydrophobic amino acid in the hydrophilic domain.
    Type: Grant
    Filed: June 10, 2016
    Date of Patent: August 4, 2020
    Assignee: Toyama Prefectual University
    Inventors: Yasuhisa Asano, Daisuke Matsui, Yuko Oku
  • Patent number: 10722525
    Abstract: The present invention provides pharmacological compounds including an effector moiety conjugated to a binding moiety that directs the effector moiety to a biological target of interest. Likewise, the present invention provides compositions, kits, and methods (e.g., therapeutic, diagnostic, and imaging) including the compounds. The compounds can be described as a protein interacting binding moiety-drug conjugate (SDC-TRAP) compounds, which include a protein interacting binding moiety and an effector moiety. For example, in certain embodiments directed to treating cancer, the SDC-TRAP can include an Hsp90 inhibitor conjugated to a cytotoxic agent as the effector moiety.
    Type: Grant
    Filed: January 19, 2017
    Date of Patent: July 28, 2020
    Assignee: MADRIGAL PHARMACEUTICALS, INC.
    Inventors: Dinesh U. Chimmanamada, Weiwen Ying
  • Patent number: 10676751
    Abstract: Disclosed herein are materials useful for degrading plant biomass material. In exemplary embodiments, the plant material comprises one or more enzymes that are expressed in plants and/or bacteria. Specifically exemplified herein are plant degrading enzymes expressed in chloroplasts. The chloroplast expressed enzymes may be provided as cocktails for use in conjunction with conventional methods of converting biomass into biofuels, such as cellulosic ethanol.
    Type: Grant
    Filed: March 2, 2009
    Date of Patent: June 9, 2020
    Assignee: The Trustees of the University of Pennsylvania
    Inventor: Henry Daniell
  • Patent number: 10670607
    Abstract: This invention relates to bioassay method wherein the presence of a specific soluble antibody in a sample of a bodily fluid, plasma or serum, of an animal, including human, is qualitatively and/or quantitatively determined. The method employs, a first group labelled with an energy donor and a second group labelled with an energy acceptor; the first group, or second group, comprising an antigen of the soluble antibody and the second group, or first group, respectively, comprising a Fab binding moiety capable of binding to a Fab region of antibodies of said animal. The donor and the acceptor form an energy transfer pair capable of energy transfer. The invention further comprises a kit for the bioassay method.
    Type: Grant
    Filed: February 27, 2015
    Date of Patent: June 2, 2020
    Assignee: University of Helsinki
    Inventors: Klaus Hedman, Jussi Hepojoki, Satu Hepojoki, Antti Vaheri, Olli Vapalahti
  • Patent number: 10669578
    Abstract: The invention relates to an improved method for characterising a template polynucleotide. The method involves using a polymerase to prepare a modified polynucleotide which makes it easier to characterise than the template polynucleotide.
    Type: Grant
    Filed: February 19, 2015
    Date of Patent: June 2, 2020
    Assignee: Oxford Nanopore Technologies Ltd.
    Inventors: James Anthony Clarke, Marion Louise Crawford, James White
  • Patent number: 10626386
    Abstract: The present invention relates to isolated polypeptides having cellulolytic enhancing activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.
    Type: Grant
    Filed: February 12, 2019
    Date of Patent: April 21, 2020
    Assignee: Novozymes, Inc.
    Inventors: Suchindra Maiyuran, Randall Kramer, Paul Harris
  • Patent number: 10626472
    Abstract: The invention relates to the use of a polysaccharide having at least four saccharide units, such as stachyose, as a glass-forming agent for the freeze-drying of a reaction mixture comprising an enzyme. In particular, the enzyme is a polymerase useful in a nucleic acid amplification reaction such as a Polymerase Chain Reaction. Compositions comprising such polysaccharides as well as methods for preparing them, kits containing them and methods for using them form further aspects of the invention.
    Type: Grant
    Filed: January 28, 2014
    Date of Patent: April 21, 2020
    Assignee: Fluorogenics Ltd
    Inventors: Martin Lee, Diane Lee, Mark Laverick
  • Patent number: RE48335
    Abstract: The present invention relates to antimicrobial compounds and their uses, and in particular to peptide antibiotics which may be used in the treatment of bacterial infections such as Gram-negative bacterial infections, particularly those caused by multidrug-resistant (MDR) pathogens.
    Type: Grant
    Filed: September 27, 2018
    Date of Patent: December 1, 2020
    Assignee: Monash University
    Inventors: Jian Li, Roger Nation, Tony Velkov, Philip Thompson, Kade D. Roberts