Enzyme (e.g., Ligases (6. ), Etc.), Proenzyme; Compositions Thereof; Process For Preparing, Activating, Inhibiting, Separating, Or Purifying Enzymes Patents (Class 435/183)
  • Patent number: 11534500
    Abstract: Angelman Syndrome (AS) is a genetic disorder occurring in approximately one in every 15,000 births. It is characterized by severe mental retardation, seizures, difficulty speaking and ataxia. The gene responsible for AS was discovered to be UBE3A and encodes for E6-AP, an ubiquitin ligase. A unique feature of this gene is that it undergoes maternal imprinting in a neuron-specific manner. In the majority of AS cases, there is a mutation or deletion in the maternally inherited UBE3A gene, although other cases are the result of uniparental disomy or mismethylation of the maternal gene. While most human disorders characterized by severe mental retardation involve abnormalities in brain structure, no gross anatomical changes are associated with AS. We have generated a Ube3a protein with additional sequences that should allow the secretion from cells and uptake by neighboring neuronal cells. This would confer a functional E6-AP protein into the neurons and rescue disease pathology.
    Type: Grant
    Filed: November 7, 2017
    Date of Patent: December 27, 2022
    Assignee: University of South Florida
    Inventors: Kevin Ron Nash, Edwin John Weeber, Jennifer Leigh Daily
  • Patent number: 11473073
    Abstract: The present disclosure provides polypeptides having aminopeptidase activity and isolated nucleic acid sequences encoding the polypeptides. In some embodiments, the disclosure also provides to nucleic acid constructs, vectors, and host cells comprising the nucleic acid sequences as well as methods for producing the polypeptides. In some embodiments, the present disclosure further provides to methods of obtaining protein hydrolysates useful as flavor improving agents.
    Type: Grant
    Filed: June 27, 2016
    Date of Patent: October 18, 2022
    Assignee: Dupont Nutrition Biosciences APS
    Inventors: Karsten Matthias Kragh, Ernst Meinjohanns, Thomas Eisele, Peter Edvard Degn, Steffen Yde Bak
  • Patent number: 11454581
    Abstract: The molecule detecting apparatus of an embodiment includes a light source 31, a fluorescent layer 42 emitting different fluorescence depending on the kind of a target molecule 60 captured when being irradiated with light from the light source 31, a photodetector 32 configured to detect fluorescence, and the photodetector 32 is an array of avalanche photodiodes operating in Geiger mode.
    Type: Grant
    Filed: August 30, 2019
    Date of Patent: September 27, 2022
    Assignee: Kabushiki Kaisha Toshiba
    Inventors: Honam Kwon, Ikuo Fujiwara, Kazuhiro Suzuki, Keita Sasaki, Yuki Nobusa, Yasushi Shinjo
  • Patent number: 11447804
    Abstract: Provided herein is a non-naturally occurring microbial organism having a methanol metabolic pathway that can enhance the availability of reducing equivalents in the presence of methanol. Such reducing equivalents can be used to increase the product yield of organic compounds produced by the microbial organism, such as adipate, 6-aminocaproate, hexamethylenediamine or caprolactam. Also provided herein are methods for using such an organism to produce adipate, 6-aminocaproate, hexamethylenediamine or caprolactam.
    Type: Grant
    Filed: November 1, 2018
    Date of Patent: September 20, 2022
    Assignee: Genomatica, Inc.
    Inventors: Anthony P. Burgard, Robin E. Osterhout, Stephen J. Van Dien, Cara Ann Tracewell, Priti Pharkya, Stefan Andrae
  • Patent number: 11414643
    Abstract: The instant disclosure is generally related to novel Bacillus sp. mutants capable of producing increased amounts of industrially relevant proteins of interest. Certain embodiments of the disclosure are related to modified Bacillus sp. cells comprising an introduced polynucleotide encoding a variant GlcT protein. Other embodiments are related to methods and compositions for producing endogenous and/or heterologous proteins of interest in the modified Bacillus sp. (daughter) cells, whereas certain other embodiments are directed to nucleic acid sequences, particularly polynucleotide open reading frame (ORF) sequences, vectors thereof and DNA expression constructs thereof, encoding variant GlcT proteins of the disclosure.
    Type: Grant
    Filed: December 12, 2018
    Date of Patent: August 16, 2022
    Assignee: DANISCO US INC.
    Inventors: Shannon Del Chase, Carol Marie Fioresi, Ryan L. Frisch, Helen Olivia Masson, Anita Van Kimmenade
  • Patent number: 11390537
    Abstract: A method and a control system for water used in a food processing system are provided. For example, the control system includes at least one sensor configured to collect a sensor signal from a produce handling device, a logic processor configured to receive the sensor signal collected by the at least one sensor and generate a control signal for controlling adding wash solution to the water used in the food processing system, and a human machine interface (HMI) configured to display information from the logic processor to a user.
    Type: Grant
    Filed: October 3, 2017
    Date of Patent: July 19, 2022
    Assignee: SMARTWASH SOLUTIONS, LLC
    Inventors: James M. Brennan, Danny Elmer Lindstrom, Christopher Michael McGinnis, Eric Child Wilhelmsen
  • Patent number: 11384359
    Abstract: Genetically engineered bacteria, pharmaceutical compositions thereof, and methods of treating or preventing autoimmune disorders, inhibiting inflammatory mechanisms in the gut, and/or tightening gut mucosal barrier function are disclosed.
    Type: Grant
    Filed: September 8, 2016
    Date of Patent: July 12, 2022
    Assignee: SYNLOGIC OPERATING COMPANY, INC.
    Inventors: Dean Falb, Vincent M. Isabella, Jonathan W. Kotula, Paul F. Miller, Yves Millet, Adam Fisher
  • Patent number: 11384033
    Abstract: A composition comprising: (A) a mixture comprising at least one (thio)phosphoric acid triamide according to the general formula (I) R1R2N—P(X)(NH2)2, wherein X is oxygen or sulfur; R1 is a C1 to C20 alkyl, C3 to C20 cycloalkyl, C6 to C20 aryl, or dialkylaminocarbonyl group; R2 is H, or R1 and R2 together with the nitrogen atom linking them define a 5- or 6-membered saturated or unsaturated heterocyclic radical, which optionally comprises 1 or 2 further heteroatoms selected from the group consisting of nitrogen, oxygen, and sulfur, and (L1) at least one amine selected from the group consisting of (L10), (L11), (L12), (L13), (L14), (L15), (L16), (L17), (L18), (L19), (L20), (L21), (L22), (L23), (L24), and (L29).
    Type: Grant
    Filed: January 22, 2020
    Date of Patent: July 12, 2022
    Assignee: BASF SE
    Inventors: Markus Schmid, Wolfram Zerulla, Gregor Pasda, Alexander Wissemeier, Tobias Lang, Karl-Heinrich Schneider, Zoltan Baan, Maarten Staal, Ansgar Gereon Altenhoff, Kian Molawi, Sophia Ebert, Olivier Fleischel
  • Patent number: 11359212
    Abstract: Compositions for expressing a polypeptide within a target organ, the composition comprising a delivery particle, and at least a first mRNA sequence complexed with, encapsulated by, or otherwise associated with the delivery particle, wherein the mRNA sequence comprises at least one coding sequence which codes for at least one polypeptide, at least a first untranslated region (UTR) sequence, and at least one micro-RNA (miRNA) binding site sequence, wherein the miRNA binding site sequence is located within, immediately 5? to, or immediately 3? to, the first UTR sequence; and wherein the miRNA binding site sequence provides for differential expression of the coding sequence between first and second cell types comprised within the target organ.
    Type: Grant
    Filed: March 6, 2020
    Date of Patent: June 14, 2022
    Assignee: Combined Therapeutics, Inc.
    Inventors: Romain Micol, Slawomir Antoszczyk
  • Patent number: 11352632
    Abstract: This present patent application relates to a method for controlling a targeted gene expression with an environmental trigger useful for manufacturing a natural product or an analogue thereof biologically. In particular, the present invention discloses an expression system comprises a gene expressing for a fusion protein of elastin-like polypeptides (ELPs) and a transcription factor, wherein a targeted gene expression is regulated by the phase change of the fusion protein initiated by an environmental trigger, including changes of temperature, pH value, ionic strength or a combination thereof. The method, the expression system and their products are within the scope of this invention.
    Type: Grant
    Filed: December 13, 2018
    Date of Patent: June 7, 2022
    Assignee: Purdue Research Foundation
    Inventors: Kevin V. Solomon, Ethan Hillman, Kok Zhi Lee, Yu Hong Wang
  • Patent number: 11338046
    Abstract: The present invention provides new adeno-associated virus-derived vectors and pharmaceutical compositions containing the same for the treatment of lysosomal storage disorders and specially, for the treatment of mucopolysaccharidoses Type IIID.
    Type: Grant
    Filed: September 22, 2017
    Date of Patent: May 24, 2022
    Assignees: ESTEVE PHARMACEUTICALS, S.A., UNIVERSITAT AUTÓNOMA DE BARCELONA
    Inventors: Carles Roca Lecha, Virginia A. Haurigot-Mendonça, Fàtima Bosch Tubert
  • Patent number: 11326197
    Abstract: Systems and methods for the detection of one or more target molecules emitted from microbial sources are described. The systems and methods may include a molecularly imprinted polymer film; a strain sensitive surface, wherein the molecularly imprinted polymer film comprises a polymer host with one or more binding sites for one or more target molecules. The molecularly imprinted polymer film may be coated upon the strain sensitive surface.
    Type: Grant
    Filed: October 29, 2013
    Date of Patent: May 10, 2022
    Assignee: FRESHAIR SENSOR, LLC
    Inventor: Joseph James Belbruno
  • Patent number: 11312747
    Abstract: A method for producing an active-form mutant enzyme, by specifying a protein of which a native form exhibits an enzyme activity but which has 10% or less enzyme activity of the native form when a gene of the protein is expressed to provide an inactive-form enzyme; determining a sequence conservation of amino acid residues in an amino acid sequence of the inactive-form enzyme and specifying amino acid residue(s) for which sequence conservation in the inactive-form enzyme is lower than sequence conservation of other amino acid(s) of the same residue; preparing a gene having a base sequence that codes for the amino acid sequence of the inactive-form enzyme in which at least one said specified amino acid residue is substituted by another amino acid with a higher sequence conservation; and expressing the gene to obtain an enzyme that exhibits an enzyme activity of the native form protein.
    Type: Grant
    Filed: May 19, 2020
    Date of Patent: April 26, 2022
    Inventors: Yasuhisa Asano, Daisuke Matsui, Yuko Oku
  • Patent number: 11198885
    Abstract: An expression increasing element comprising a 36 nucleotide DNA sequence that can be used to increase the expression of genes, and in particular to increase the expression of endogenous plant genes, in plants is disclosed. Also disclosed are plants, plant parts, and commodity plant products comprising the expression increasing element along with related methods of using the expression increasing element and plants comprising the expression increasing element.
    Type: Grant
    Filed: March 2, 2021
    Date of Patent: December 14, 2021
    Assignee: Inari Agriculture Technology, Inc.
    Inventor: Hannes Bart Claeys
  • Patent number: 11174487
    Abstract: Methods of constructing a cell comprising in its chromosome one or more copies of an open reading frame (ORF) or operon encoding at least one polypeptide of interest, each copy being under the transcriptional control of a heterologous promoter using a site specific recombinase and in vivo integration by recombination; resulting cells, and methods for producing a polypeptide of interest using the resulting cells.
    Type: Grant
    Filed: April 7, 2017
    Date of Patent: November 16, 2021
    Assignee: Novozymes A/S
    Inventors: Anne Breuner, Michael Dolberg Rasmussen
  • Patent number: 11172657
    Abstract: Provided is a method for developing a secondary organ by using a non-human animal in which organ formation is inhibited, for the purpose of establishing a process for producing a functional cell such as a ? cell within the body of an animal such as a pig, the method including the step of raising a newborn or a fetus of the non-human animal in which organ formation is inhibited by complementing at least a part of the function of the organ whose formation is inhibited.
    Type: Grant
    Filed: September 18, 2018
    Date of Patent: November 16, 2021
    Assignee: PORMEDTEC CO., LTD.
    Inventors: Hiroshi Nagashima, Hitomi Matsunari, Kazuaki Nakano, Koki Hasegawa
  • Patent number: 11174297
    Abstract: An object of the present invention is to identify an enzyme having activity of synthesizing dihydrotentoxin that is a tentoxin precursor and an enzyme having activity of synthesizing tentoxin using dihydrotentoxin as a substrate. The present invention concerns a tentoxin synthesis-related gene encoding a protein comprising the amino acid sequence of SEQ ID NO: 16 and having activity of nonribosomal peptide synthesis of dihydrotentoxin and a tentoxin synthesis-related gene encoding a protein comprising the amino acid sequence of SEQ ID NO: 18 and having activity of converting dihydrotentoxin to tentoxin.
    Type: Grant
    Filed: March 16, 2017
    Date of Patent: November 16, 2021
    Assignee: KUMIAI CHEMICAL INDUSTRY CO., LTD.
    Inventors: Takashi Kubo, Masayuki Machida, Tomonori Fujioka, Shigenari Yamaguchi, Kiyoshi Kawai
  • Patent number: 11155801
    Abstract: Unglycosylated lysostaphin variant protein, nucleic acid molecule, vector and host cell, as well as a method for production of unglycosylated lysostaphin variant protein in a yeast expression system are provided. The proteins are produced in a Pichia pastoris expression system and have been shown to have activity equivalent to wild-type lysostaphin. The lysostaphin variant proteins can be used as therapeutic proteins for treatment of diseases such as Staphylococcus aureus infection.
    Type: Grant
    Filed: July 16, 2014
    Date of Patent: October 26, 2021
    Assignee: Trustees of Dartmouth College
    Inventors: Karl E. Griswold, Hongliang Zhao
  • Patent number: 11149293
    Abstract: This invention provides a fermentation method of producing gellan gum, wherein a certain amount of fermentation broth is retained in the fermentor as a seed for next batch fermentation or transferred to another fermentor as a seed for next batch fermentation in that fermentor. The fermentation method of this invention reduces fermentation cost of gellan gum and lowers contamination risk during seed cultivation.
    Type: Grant
    Filed: November 1, 2018
    Date of Patent: October 19, 2021
    Assignees: Zhejiang DSM Zhongken Biotechnology Co. Ltd, DSM IP Assets B.V.
    Inventors: Jie Zhao, Wouter Adrianus Van Winden, Rogier Meulenberg, Zhihui Gao
  • Patent number: 11130946
    Abstract: The present invention relates to variants of a parent glucoamylase having altered properties (e.g., improved thermostability and/or specific activity). In particular, the present invention provides compositions comprising the variant glucoamylases, including starch hydrolyzing compositions, animal feed compositions and cleaning compositions. The invention also relates to DNA constructs encoding the variants and methods of producing the glucoamylase variants in host cells.
    Type: Grant
    Filed: January 14, 2020
    Date of Patent: September 28, 2021
    Assignee: Danisco US Inc.
    Inventors: Pieter Van Solingen, Richard R. Bott, Wolfgang Aehle, Martijn Scheffers
  • Patent number: 11091785
    Abstract: The present invention relates to enzyme compositions for high temperature saccharification of cellulosic material and to uses thereof.
    Type: Grant
    Filed: April 3, 2020
    Date of Patent: August 17, 2021
    Assignee: Novoyzmes, Inc.
    Inventors: Brett McBrayer, Tarana Shaghasi, Elena Vlasenko
  • Patent number: 11085048
    Abstract: The present invention provides an expression cassette containing a polynucleotide coding sequence for a hydroxycinnamoyl-CoA hydratase-lyase (HCHL), which is operably linked to a heterologous promoter. Also provided are methods of engineering plants that have reduced lignification, as well as cells, plant parts, and plant tissues from such engineered plants.
    Type: Grant
    Filed: April 26, 2019
    Date of Patent: August 10, 2021
    Assignee: The Regents of the University of California
    Inventors: Dominique Loqué, Aymerick Guillaume Eudes
  • Patent number: 11072783
    Abstract: An isolated and/or purified ?-glucanotransferase from Exiguobacterium Acetylicum, recombinantly engineered variants thereof, active fragments thereof, synthetic nucleic acids encoding the ?-glucanotransferase and variants thereof, host cells comprising the synthetic nucleic acids, and compositions comprising the ?-glucanotransferase are provided. Methods of using the compositions include the manufacture of oligosaccharides.
    Type: Grant
    Filed: October 6, 2016
    Date of Patent: July 27, 2021
    Assignee: DUPONT NUTRITION BIOSCIENCES APS
    Inventor: Slavko Kralj
  • Patent number: 11073518
    Abstract: The present invention relates to a prefabricated microparticle for performing detection, preferably a digital detection and/or quantitation of an analyte. Furthermore, it also relates to a detection and/or quantitation of multiple analytes by prefabricated microparticles. It also relates to a collection of such prefabricated microparticles and to the use of such microparticle(s) and/or of such collection. Furthermore, the present invention also relates to a method of performing a detection and/or quantitation of an analyte in a sample wherein a microparticle or collection of microparticles are used. In one embodiment, in the collection of microparticles, individual microparticles are tailored for the detection of specific analytes and can be distinguished from each other by a specific label indicating the respective analyte for which the individual microparticle is specific.
    Type: Grant
    Filed: December 22, 2017
    Date of Patent: July 27, 2021
    Assignee: BLINK AG
    Inventors: Katrin Steinmetzer, Stephan Hubold, Thomas Ellinger, Eugen Ermantraut, Torsten Schulz
  • Patent number: 11066655
    Abstract: Cyanophage Syn5 RNA polymerase (RNAP) and methods of purifying it are provided. Cyanophage Syn5 RNAP having one or more promoter mutations are provided. Methods of expressing cyanophage Syn5 RNAP are provided. Novel promoter sequences and in vitro transcription systems utilizing Syn5 RNAP are provided. Methods of making high range ssRNA ladders are also provided. Methods of incorporating 2?-modified dNTPs using Syn5 RNA polymerase are provided.
    Type: Grant
    Filed: February 12, 2016
    Date of Patent: July 20, 2021
    Assignee: President and Fellows of Harvard College
    Inventors: Charles C. Richardson, Bin Zhu, Stanley Tabor
  • Patent number: 11034938
    Abstract: There is provided a microbial cell expressing a mutant AlkB enzyme, the mutant AlkB enzyme comprising at least one point mutation in the wild type sequence of AlkB, wherein the point mutation is at amino acid position V129 and/or T136 of the wild type AlkB enzyme. There is also provided a method for producing omega-hydroxy carboxylic acid and/or ester thereof using this cell.
    Type: Grant
    Filed: July 3, 2017
    Date of Patent: June 15, 2021
    Assignee: EVONIK OPERATIONS GMBH
    Inventors: Arne Skerra, Ludwig Kirmair, Steffen Schaffer, Christoph Schorsch, Mirja Wessel, Thomas Haas
  • Patent number: 11021726
    Abstract: The invention relates to a method for the production of a solid bio-product wherein at least 80% of the original indigestible oligosaccharide (raffmose, stachyose and verbascose) content has been degraded into digestible mono- and disaccharides, comprising the following steps: 1) providing a mixture of milled or flaked or otherwise disintegrated biomass, comprising oligosaccharides and optionally polysaccharides and further comprising proteinaceous plant parts, water and one or more enzyme preparations containing .alpha.-galactosidase(s); 2) reacting the mixture resulting from step (1) under continuous mixing and under conditions where the water content in the initial mixture does not exceed 65% by weight, for 0.15-36 hours at a temperature of about 20-65° C.; 3) incubating the reacted mixture from step (2) at a temperature and in a time period which inactivate said ?-galactosidase(s), as well as solid bio-products obtainable by such method.
    Type: Grant
    Filed: May 23, 2017
    Date of Patent: June 1, 2021
    Assignee: HAMLET PROTEIN A/S
    Inventors: Katrine Hvid Ellegard, Karl Kristian Thomsen, Ole Kaae Hansen
  • Patent number: 11021729
    Abstract: The present invention provides engineered ketoreductase and phosphite dehydrogenase enzymes having improved properties as compared to a naturally occurring wild-type ketoreductase and phosphite dehydrogenase enzymes, as well as polynucleotides encoding the engineered ketoreductase and phosphite dehydrogenase enzymes, host cells capable of expressing the engineered ketoreductase and phosphite dehydrogenase enzymes, and methods of using the engineered ketoreductase and phosphite dehydrogenase enzymes to synthesize a chiral catalyst used in the synthesis of antiviral compounds, such as nucleoside inhibitors. The present invention further provides methods of using the engineered enzymes to deracemize a chiral alcohol in a one-pot, multi-enzyme system.
    Type: Grant
    Filed: April 13, 2018
    Date of Patent: June 1, 2021
    Assignee: Codexis, Inc.
    Inventors: Jeffrey C. Moore, Jack Liang, Jonathan Penfield, Jovana Nazor, Nikki Dellas, Vesna Mitchell, Da Duan, Iman Farasat, Agustina Rodriguez-Granillo, Grant Murphy, Nicholas Marshall
  • Patent number: 11015149
    Abstract: Bioactive coatings that include a base and a protein associated with the base for actively promoting the removal of organic stains are provided. In aspects, bioactive coatings that are stabilized against inactivation by weathering are provided including a base associated with a chemically modified enzyme, and, optionally a first polyoxyethylene present in the base and independent of the enzyme. The coatings are optionally overlayered onto a substrate to form an active coating facilitating the removal of organic stains or organic material from food, insects, or the environment.
    Type: Grant
    Filed: November 13, 2017
    Date of Patent: May 25, 2021
    Assignees: Toyota Motor Corporation, Regents of the University of Minnesota
    Inventors: Andreas Buthe, Ping Wang, Songtao Wu, Hongfei Jia, Masahiko Ishii, Minjuan Zhang
  • Patent number: 10988738
    Abstract: The present invention provides a microorganism-derived soluble coenzyme-binding glucose dehydrogenase which catalyzes a reaction for oxidizing glucose in the presence of an electron acceptor, has an activity to maltose as low as 5% or less, and is inhibited by 1,10-phenanthroline. The invention also provides a method for producing the coenzyme-binding glucose dehydrogenase, and a method and a reagent for measuring employing the coenzyme-binding glucose dehydrogenase. According to the invention, the coenzyme-binding glucose dehydrogenase can be applied to an industrial field, and a use becomes possible also in a material production or analysis including a method for measuring or eliminating glucose in a sample using the coenzyme-binding glucose dehydrogenase as well as a method for producing an organic compound. It became also possible to provide a glucose sensor capable of accurately measuring a blood sugar level.
    Type: Grant
    Filed: February 15, 2019
    Date of Patent: April 27, 2021
    Assignees: Ikeda Food Research Co., Ltd., PHC Corporation
    Inventors: Hironori Omura, Hirokazu Sanada, Takako Yada, Tetsunari Morita, Mika Kuyama, Tokuji Ikeda, Kenji Kano, Seiya Tsujimura
  • Patent number: 10988714
    Abstract: Bioactive coatings that include a base and a protein associated with the base for actively promoting the removal of organic stains are provided. In aspects, bioactive coatings that are stabilized against inactivation by weathering are provided including a base associated with a chemically modified enzyme, and, optionally a first polyoxyethylene present in the base and independent of the enzyme. The coatings are optionally overlayered onto a substrate to form an active coating facilitating the removal of organic stains or organic material from food, insects, or the environment.
    Type: Grant
    Filed: November 13, 2017
    Date of Patent: April 27, 2021
    Assignees: Regents of the University of Minnesota, Toyota Motor Corporation
    Inventors: Andreas Buthe, Ping Wang, Songtao Wu, Hongfei Jia, Masahiko Ishii, Minjuan Zhang
  • Patent number: 10973887
    Abstract: Methods to introduce highly phosphorylated mannopyranosyl oligosaccharide derivatives containing mannose-6-phosphate (M6P), or other oligosaccharides bearing other terminal hexoses, to carbonyl groups on oxidized glycans of glycoproteins while retaining their biological activity are described. The methods are useful for modifying glycoproteins, including those produced by recombinant protein expression systems, to increase uptake by cell surface receptor-mediated mechanisms, thus improving their therapeutic efficacy in a variety of applications.
    Type: Grant
    Filed: June 12, 2019
    Date of Patent: April 13, 2021
    Assignee: GENZYME CORPORATION
    Inventor: Yunxiang Zhu
  • Patent number: 10941185
    Abstract: The present invention discloses a strain of bacteria producing DHA and/or EPA, six gene fragments in the bacterial genome, and uses thereof. The strain is Schizoochytrium limacinum HS01, which has the accession number of CGMCC No. 13746 at China General Microbiological Culture Collection Center. The six gene fragments are composed of gene fragment 1 to gene fragment 6, and the nucleotide sequences are sequentially as shown in SEQ ID NO: 3 to SEQ ID NO: 8 in the Sequence Listing. The experiments prove that fermentation broth containing DHA and EPA can be obtained by fermenting Schizoochytrium limacinum HS01; the recombinant strain is obtained by introducing gene fragment 1 to gene fragment 6 into Schizoochytrium limacinum MYA-1381; the ability the recombinant strain for producing DHA and EPA is greatly improved.
    Type: Grant
    Filed: November 26, 2019
    Date of Patent: March 9, 2021
    Assignee: XIAMEN HUISON BIOTECH CO., LTD.
    Inventors: Liyi Chen, Huichang Zhong, Shuirong Chen
  • Patent number: 10934324
    Abstract: A method of improving protein functionality includes obtaining a protein such as protein contained within exclusion bodies contained within bacteria or yeast used for the recombinant expression of the protein. The exclusion bodies are solubilized using a chaotropic agent such as guanidine or urea. The protein is then subject to denaturation conditions and optionally purified. A liquid containing the denatured protein is loaded into a vessel that is angled relative to horizontal. The vessel is then rotated in the angled configuration at a rate within that is less than 10,000 RPM for period of time. Refolded protein is formed by the high shear conditions formed in the thin film of fluid formed in the inner surface of the rotating vessel. Refolding can be performed in a batch mode or a continuous mode. The process may be scaled up for industrial applications by using multiple vessels.
    Type: Grant
    Filed: September 3, 2014
    Date of Patent: March 2, 2021
    Assignee: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
    Inventors: Gregory A. Weiss, Colin L. Raston, Tom Z. Yuan, Callum Ormonde, Stephan Timothy Kudlacek
  • Patent number: 10925293
    Abstract: The present invention relates to methods of producing lipids. In particular, the present invention relates to methods of increasing the level of one or more non-polar lipids and/or the total non-polar lipid content in a transgenic organism or part thereof. In one particular embodiment, the present invention relates to the use of an acyltransferase, for example, a monoacylglycerol acyltransferase (MGAT) to increase the level of one or more non-polar lipids and/or the total non-polar lipid content in plants, plant seed and/or leaves, algae and fungi.
    Type: Grant
    Filed: August 25, 2015
    Date of Patent: February 23, 2021
    Assignee: Commonwealth Scientific and Industrial Research Organisation
    Inventors: James Robertson Petrie, Thomas Vanhercke, Pushkar Shrestha, Qing Liu, Surinder Pal Singh
  • Patent number: 10900045
    Abstract: The present invention provides a novel fluorescent protein. A novel protein includes the following protein (F1): (F1) a protein consisting of an amino acid sequence of SEQ ID NO: 1, wherein in the amino acid sequence of SEQ ID NO: 1, a 52nd amino acid Xaa52 is an arbitrary amino acid, a 133rd amino acid Xaa133 is an arbitrary amino acid, and a 154th amino acid Xaa154 is an arbitrary amino acid.
    Type: Grant
    Filed: June 13, 2016
    Date of Patent: January 26, 2021
    Assignee: NEC Solution Innovators. Ltd.
    Inventors: Ikuo Shiratori, Akihisa Shimizu, Katsunori Horii, Hiroshi Mishima, Iwao Waga
  • Patent number: 10883069
    Abstract: Bioactive coatings that include a base and a protein associated with the base for actively promoting the removal of organic stains are provided. In aspects, bioactive coatings that are stabilized against inactivation by weathering are provided including a base associated with a chemically modified enzyme, and, optionally a first polyoxyethylene present in the base and independent of the enzyme. The coatings are optionally overlayered onto a substrate to form an active coating facilitating the removal of organic stains or organic material from food, insects, or the environment.
    Type: Grant
    Filed: November 13, 2017
    Date of Patent: January 5, 2021
    Assignees: Toyota Motor Corporation, Regents of the University of Minnesota
    Inventors: Andreas Buthe, Ping Wang, Songtao Wu, Hongfei Jia, Masahiko Ishii, Minjuan Zhang
  • Patent number: 10883094
    Abstract: Transposase polypeptides and polynucleotides are provided, which have a high activity in mammalian cells. Methods for engineering cells, such as chimeric antigen T-cells, with the transposes are also provided.
    Type: Grant
    Filed: January 14, 2020
    Date of Patent: January 5, 2021
    Assignee: BOARD OF REGENTS, THE UNIVERSITY OF TEXAS SYSTEM
    Inventors: Laurence J. N. Cooper, Natalya Belousova
  • Patent number: 10869494
    Abstract: Providing a D-psicose-containing sweetener with the modification of the taste of D-psicose, comprising D-psicose, a sugar alcohol and/or a high intensity sweetener, preferably containing D-psicose as the main component, particularly a low-calorie sweetener and/or a sweetener giving refreshing feel in the oral cavity, as well as foods and drinks obtained by using the D-psicose-containing sweetener with the modification of the taste of D-psicose, and other products given with sweetness. The sugar alcohol is one or more sugar alcohols selected from the group consisting of sorbitol, mannitol, lactitol, maltitol, xylitol and erythritol, while the high intensity sweetener is one or more high intensity sweeteners as selected from aspartame, acesulfame K, sodium cyclamate, sodium saccharin, Sucralose (under trade name), stevia sweetener, dulcin, taumatin, neotame and monellin.
    Type: Grant
    Filed: March 26, 2007
    Date of Patent: December 22, 2020
    Assignee: MATSUTANI CHEMICAL INDUSTRY CO., LTD.
    Inventors: Hideki Fujihara, Kazuhiro Okuma, Tetsuo Iida, Ken Izumori, Masaaki Tokuda, Kazuhiro Fukada
  • Patent number: 10829770
    Abstract: Polynucleotides encoding fusion proteins contain a secretable luciferase fused to a modified polypeptide of interest are disclosed. The polypeptide of interest has been modified to remove a native N-terminal secretion sequence and has been replaced by the secretable luciferase. One example of a modified polypeptide of interest is interferon. The polynucleotides and fusion proteins have biotherapeutic, diagnostic, and quality control applications in biotechnological, medical, and veterinary fields. Methods for producing the secretable fusion protein are also disclosed.
    Type: Grant
    Filed: September 16, 2019
    Date of Patent: November 10, 2020
    Assignee: The Government of the United States of America, as represented by the Secretary of Homeland Security
    Inventors: Michael Puckette, Max V. Rasmussen
  • Patent number: 10800848
    Abstract: Disclosed herein are compositions and pharmaceutical formulations that comprise a binding moiety conjugated to a polynucleic acid molecule and a polymer. Also described herein include methods for treating a cancer which utilize a composition or a pharmaceutical formulation comprising a binding moiety conjugated to a polynucleic acid molecule and a polymer.
    Type: Grant
    Filed: September 11, 2018
    Date of Patent: October 13, 2020
    Assignee: AVIDITY BIOSCIENCES, INC.
    Inventors: Andrew John Geall, Venkata Ramana Doppalapudi, David Sai-Ho Chu, Michael Caramian Cochran, Rachel Elizabeth Johns, Palani Balu, Rob Burke, Beatrice Diana Darimont
  • Patent number: 10792341
    Abstract: Methods of treating glycogen storage disease type II, by administering acid ?-glucosidase, are described, as are compositions for use in treatment of glycogen storage disease type II.
    Type: Grant
    Filed: January 19, 2018
    Date of Patent: October 6, 2020
    Assignee: Duke University
    Inventor: Yuan-Tsong Chen
  • Patent number: 10793922
    Abstract: The present invention provides methods for rapidly identifying an RNA viral infection using an isothermal nucleic acid amplification reaction that can be carried out extracted RNA in the context of a crude biological sample.
    Type: Grant
    Filed: October 20, 2015
    Date of Patent: October 6, 2020
    Assignee: ENVIROLOGIX INC.
    Inventors: Lars Peters, Stephen A. Judice, Daniel Shaffer, Breck Parker
  • Patent number: 10787519
    Abstract: Disclosed herein are compositions and pharmaceutical formulations that comprise a binding moiety conjugated to a polynucleic acid molecule and a polymer. Also described herein include methods for treating a cancer which utilize a composition or a pharmaceutical formulation comprising a binding moiety conjugated to a polynucleic acid molecule and a polymer.
    Type: Grant
    Filed: September 11, 2018
    Date of Patent: September 29, 2020
    Assignee: AVIDITY BIOSCIENCES, INC.
    Inventors: Andrew John Geall, Venkata Ramana Doppalapudi, David Sai-Ho Chu, Michael Caramian Cochran, Rachel Elizabeth Johns, Palani Balu, Rob Burke, Beatrice Diana Darimont
  • Patent number: 10786798
    Abstract: The present invention relates to a method for preparing solid capsules comprising a compound A, dispersed in a composition C4.
    Type: Grant
    Filed: January 27, 2016
    Date of Patent: September 29, 2020
    Assignee: CALYXIA
    Inventors: Jamie Dean Walters, Jérôme Bibette
  • Patent number: 10767226
    Abstract: A honeycomb tube with a planar frame defining a fluidic path between a first planar surface and a second planar surface. A fluidic interface is located at one end of the planar frame. The fluidic interface has a fluidic inlet and fluidic outlet. The fluidic path further includes a well chamber having an well-substrate with a plurality of wells. The well chamber is arranged in the planar frame between the first or second surface and the well-substrate.
    Type: Grant
    Filed: December 21, 2018
    Date of Patent: September 8, 2020
    Assignee: Cepheid
    Inventors: Yuh-Min Chiang, Douglas B Dority, Dustin Dickens, Jennifer Glass, Reuel Van Atta
  • Patent number: 10767211
    Abstract: This invention provides an amadoriase having high reactivity with ?-fructosyl hexapeptide (?F6P) derived from the ?-chain amino terminus of glycated hemoglobin (HbAlc), which enables satisfactory quantification of ?F6P cleaved from HbAlc. A novel amadoriase is derived from the amadoriase of the genus Coniochaeta by substitution of one or more amino acids at positions selected from the group consisting of positions 62, 63, 102, 106, 110, 113, 355, and 419. Such amadoriase enables rapid, simple, accurate, and satisfactory quantification of ?F6P cleaved from HbAlc. With the use of such amadoriase, a method for measurement of HbAlc by an enzymatic method and a kit for measurement of HbAlc that are based on the same principle as used in the conventional standard method and excellent in terms of the relevance with the standard method.
    Type: Grant
    Filed: April 26, 2013
    Date of Patent: September 8, 2020
    Assignee: Kikkoman Corporation
    Inventors: Atsushi Ichiyanagi, Yosuke Masakari
  • Patent number: 10767206
    Abstract: The invention provides a new fermentation process for producing gellan gum, which can control the feeding amount and feeding speed of the nitrogen sources accurately and quantitatively, and control the growth and gum production of the strains, so as to significantly improve the controllability, stability and yield of the fermentation process for producing gellan gum.
    Type: Grant
    Filed: June 19, 2018
    Date of Patent: September 8, 2020
    Assignee: DSM IP ASSETS B.V.
    Inventors: Jie Zhao, Zhihui Gao, Wouter Adrianus Van Winden, Rogier Meulenberg
  • Patent number: 10767204
    Abstract: The present disclosure provides methods and systems for generating sugars from biomass. The methods and systems provide increased yields of carbohydrates and fermentable sugars by combining enzyme recycling with removal of saccharification enzyme inhibitors and increased solids loading.
    Type: Grant
    Filed: December 20, 2013
    Date of Patent: September 8, 2020
    Assignee: Edeniq, Inc.
    Inventors: James Kacmar, Rich Kleinke, Bernard Cooker, Chris Kalinowski, Jon Van Volkinburg
  • Patent number: RE48335
    Abstract: The present invention relates to antimicrobial compounds and their uses, and in particular to peptide antibiotics which may be used in the treatment of bacterial infections such as Gram-negative bacterial infections, particularly those caused by multidrug-resistant (MDR) pathogens.
    Type: Grant
    Filed: September 27, 2018
    Date of Patent: December 1, 2020
    Assignee: Monash University
    Inventors: Jian Li, Roger Nation, Tony Velkov, Philip Thompson, Kade D. Roberts