Lyase (4. ) Patents (Class 435/232)
  • Hydroperoxide lyase polypeptides
    Publication number: 20010007156
    Abstract: Compositions and methods for enhancing disease resistance, and modulating levels of flavor molecules in plants are provided. The compositions comprise nucleotide sequences encoding maize HPL genes. The methods comprise expressing hydroperoxide lyase (HPL) genes in plants, plant cells, and plant tissues. Expression cassettes comprising the HPL sequences of the invention are provided, as well as transformed plant cells, plant tissues, and plants.
    Type: Application
    Filed: February 8, 2001
    Publication date: July 5, 2001
    Applicant: Pioneer Hi-Bred International, Inc.
    Inventors: Jon Duvick, Jacob Gilliam
  • yitJ
    Patent number: 6251652
    Abstract: The invention provides yitJ polypeptides and DNA (RNA) encoding yitJ polypeptides and methods for producing such polypeptides by recombinant techniques. Also provided are methods for utilizing yitJ polypeptides to screen for antibacterial compounds.
    Type: Grant
    Filed: January 28, 1999
    Date of Patent: June 26, 2001
    Assignees: SmithKline Beecham Corporation, SmithKline Beecham plc
    Inventors: Michael Terence Black, John Edward Hodgson, David Justin Charles Knowles, Raymond Winfield Reichard, Richard Oakley Nicholas, Martin Karl Russel Burnham, Jamie M Pratt, Martin Rosenberg, Judith M Ward, Michael Arthur Lonetto, Patrick Vernon Warren
  • Human Oxalyl-CoA Decarboxylase
    Publication number: 20010003743
    Abstract: A human oxalyl-CoA decarboxylase polypeptide and DNA(RNA) encoding such polypeptide and a procedure for producing such polypeptide by recombinant techniques and for producing an antibody against such polypeptide are disclosed. Also disclosed is a combination of the polypeptide of the present invention and a suitable pharmaceutical carrier for providing a therapeutically effective amount of the polypeptide for the treatment of urolithiasis and hyperoxaluria. Also disclosed are assays for identifying mutations in nucleic acid sequence encoding a polypeptide of the present invention and for detecting altered levels of the polypeptide of the present invention.
    Type: Application
    Filed: December 8, 2000
    Publication date: June 14, 2001
    Applicant: Human Genome Sciences, Inc.
    Inventors: Henrik S. Olsen, Timothy A. Coleman, Mark D. Adams
  • Method for producing amide compounds using a nitrile hydratase from a thermophilic bacillus
    Patent number: 6242242
    Abstract: A process for the bioconversion of a nitrile to its corresponding amide product, particularly acrylonitrile to acrylamide which is used for forming polymers. The process uses a thermophilic bacterium having a nitrile hydratase activity that is constitutively expressed, activated by cobalt ions, stable at 60° C., and is most active between 20° C. to 70° C. with optimum activity at 55° C. Alternatively, the process uses the enzyme extracted from the thermophilic bacterium to convert a nitrile to its amide product. The genes encoding nitrile hydratase and amidase are described in which the former is useful for the conversion of an nitrile to its amide and the later is useful for the conversion of an amide to its acid.
    Type: Grant
    Filed: April 13, 2000
    Date of Patent: June 5, 2001
    Assignee: Board of Trustees operating Michigan State University
    Inventors: Patrick J. Oriel, Rugmini Padmakumar, Sang Hoon Kim
  • Hydroperoxide lyases
    Patent number: 6238898
    Abstract: The present invention relates to the production of HPO lyase proteins in hosts via recombinant expression of said proteins. Recombinant HPO lyase proteins, DNA sequences encoding these proteins, vectors containing these DNA sequences and hosts containing these vectors are provided, along with methods for recombinantly producing such proteins, DNA sequences, vectors and hosts. Also provided are processes for producing green note compounds.
    Type: Grant
    Filed: October 14, 1999
    Date of Patent: May 29, 2001
    Assignee: Givaudan Roure (International) SA
    Inventors: Alex Häusler, Konrad Lerch, Andreas Muheim, Natasha Silke
  • Methioninase formulations and use in diagnostic methods
    Patent number: 6231854
    Abstract: The present invention discloses the cloning of a methioninase-encoding nucleic acid molecule from Pseudomonas putida and the construction of high-level expression modules containing the methioninase-encoding nucleic acid molecule. The invention further provides expression modules that use the T7 RNA polymerase promoter to express the isolated methioninase-encoding nucleic acid molecules. Expression modules employing the T7 promoter were found to produce unexpectedly high levels of methioninase. The present invention further provides purification methods to obtain highly pure, endotoxin free methioninase, chemically modified forms of methioninase, crystallized methioninase and lyophilized methioninase preparations. The present invention further provides therapeutical methods using the disclosed recombinant methioninase preparations.
    Type: Grant
    Filed: March 2, 1999
    Date of Patent: May 15, 2001
    Assignee: Anticancer, Inc.
    Inventor: Tan Yuying
  • Method for producing amide compounds using a nitrile hydratase from a thermophilic bacillus
    Patent number: 6228633
    Abstract: A process for the bioconversion of a nitrile to its corresponding amide product, particularly acrylonitrile to acrylamide which is used for forming polymers. The process uses a thermophilic bacterium having a nitrile hydratase activity that is constitutively expressed, activated by cobalt ions, stable at 60° C., and is most active between 20° C. to 70° C. with optimum activity at 55° C. Alternatively, the process uses the enzyme extracted from the thermophilic bacterium to convert a nitrile to its amide product. The genes encoding nitrile hydratase and amidase are described in which the former is useful for the conversion of an nitrile to its amide and the later is useful for the conversion of an amide to its acid.
    Type: Grant
    Filed: April 13, 2000
    Date of Patent: May 8, 2001
    Assignee: Board of Trustees operating Michigan State University
    Inventors: Patrick J. Oriel, Rugmini Padmakumar, Sang Hoon Kim
  • Enzymatic process for the preparation of (S)-cyanohydrins
    Patent number: 6225095
    Abstract: Process for the preparation of the (S)-enantiomers of optically active cyanohydrins, in which a reaction mixture of a) an aldehyde or ketone dissolved in an organic, water-immiscible or slightly miscible diluent, b) an aqueous (S)-hydroxynitrile lyase solution and c) a cyanide group donor is stirred in such a way that an emulsion is formed which is maintained up to the end of the reaction by stirring.
    Type: Grant
    Filed: December 29, 1998
    Date of Patent: May 1, 2001
    Assignee: DSM Fine Chemicals Austria GmbH
    Inventors: Peter Pöchlauer, Michael Schmidt, Irma Wirth, Rudolf Neuhofer, Antonia Zabelinskaja-Mackova, Herfried Griengl, Cor Van den Broek, Raf Reintjens, Herman Jelle Wories
  • Rationally designed polysaccharide lyases derived from heparinase I
    Patent number: 6217863
    Abstract: Modified heparinases having altered binding specificity and activity are provided. Isolated nucleic acids encoding the same as as vectors and host cells are provided. Methods for using the modified heparinases are also provided.
    Type: Grant
    Filed: January 19, 1999
    Date of Patent: April 17, 2001
    Assignee: Massachusetts Institute of Technology
    Inventors: Ranganathan Godavarti, Ram Sasisekharan, Steffen Ernst, Ganesh Venkataraman, Charles L. Cooney, Robert Langer
  • Method for producing amide compounds using a nitrile hydratase from a thermophilic bacillus
    Patent number: 6214603
    Abstract: A process for the bioconversion of a nitrile to its corresponding amide product, particularly acrylonitrile to acrylamide which is used for forming polymers. The process uses a thermophilic bacterium having a nitrile hydratase activity that is constitutively expressed, activated by cobalt ions, stable at 60° C., and is most active between 20° C. to 70° C. with optimum activity at 55° C. Alternatively, the process uses the enzyme extracted from the thermophilic bacterium to convert a nitrile to its amide product. The genes encoding nitrile hydratase and amidase are described in which the former is useful for the conversion of an nitrile to its amide and the later is useful for the conversion of an amide to its acid.
    Type: Grant
    Filed: April 13, 2000
    Date of Patent: April 10, 2001
    Assignee: Board of Trustees operating Michigan State University
    Inventors: Patrick J. Oriel, Rugmini Padmakumar, Sang Hoon Kim
  • Method for producing L-aspartic acid
    Patent number: 6214589
    Abstract: This invention relates to a method for producing L-aspartic acid, comprising the steps of: immobilizing microbial cells containing aspartase to produce an immobilized aspartase; feeding an ammonium fumarate solution into a reactor filled with the immobilized aspartase; and recovering the produced L-aspartic acid from the reaction mixture, wherein the immobilized aspartase has an activity of 250 U or more, and wherein the ammonium fumarate solution is fed into the reactor at the feed rate LHSV of 2 to 35.
    Type: Grant
    Filed: February 12, 1999
    Date of Patent: April 10, 2001
    Assignee: Nippon Shokubai Co., Ltd.
    Inventors: Masaharu Mukouyama, Satomi Komatsuzaki
  • Development of genetically engineered bacteria for production of selected aromatic compounds
    Patent number: 6210937
    Abstract: The cloning and expression of genes in the common aromatic pathway of E. coli are described. A compound for which chorismate, the final product of the common aromatic pathway, is an anabolic intermediate can be produced by cloning and expressing selected genes of the common aromatic pathway and the genes coding for enzymes necessary to convert chorismate to the selected compound. Plasmids carrying selected genes of the common aromatic pathway are also described.
    Type: Grant
    Filed: April 22, 1998
    Date of Patent: April 3, 2001
    Assignee: Bechtel BWXT Idaho, LLC
    Inventors: Thomas E. Ward, Carolyn S. Watkins, Deborah K. Bulmer, Bruce F. Johnson, Mohan Amaratunga
  • Method for preparing a product in particular for preventing and curing skin diseases, and resulting product
    Patent number: 6207438
    Abstract: The invention relates to a process for the preparation of a product and to the product itself to prevent and care for skin disorders. The process includes using a natural sodium alginate extract, and the submission of this extract to at least on depolymerization treatment. The resulting product includes oligo-alginate chains and uronic acids. The product is used to treat cutaneous rashes, allergic reactions to cosmetic compositions, and the cutaneous effects of aging.
    Type: Grant
    Filed: May 5, 1998
    Date of Patent: March 27, 2001
    Assignee: Codif International SA
    Inventors: Jean Gedouin, Romuald Vallee
  • ATP eliminator and the process for determining biological cells
    Patent number: 6200767
    Abstract: The present invention provides a process for eliminating effectively ATP in a sample, using adenosine phosphate deaminase alone or in combination with at least one enzyme from the group consisting of apyrase, alkaline phosphatase, acid phosphatase, hexokinase and adenosine triphosphatase, a process for determining biological cells contained in foods and beverages in convenient and precise manner in combination with bioluminescence method, and a reagent for the determination thereof.
    Type: Grant
    Filed: January 5, 1999
    Date of Patent: March 13, 2001
    Assignee: Kikkoman Corporation
    Inventors: Tatsuya Sakakibara, Seiji Murakami, Noriaki Hattori, Keiko Yajitate, Teruo Watarai, Motoo Nakajima, Kazuhiro Imai
  • Human oxalyl-CoA decarboxylase
    Patent number: 6200796
    Abstract: A human oxalyl-CoA decarboxylase polypeptide and DNA (RNA) encoding such polypeptide and a procedure for producing such polypeptide by recombinant techniques and for producing an antibody against such polypeptide are disclosed. Also disclosed is a combination of the polypeptide of the present invention and a suitable pharmaceutical carrier for providing a therapeutically effective amount of the polypeptide for the treatment of urolithiasis and hyperoxaluria. Also disclosed are assays for identifying mutations in nucleic acid sequence encoding a polypeptide of the present invention and for detecting altered levels of the polypeptide of the present invention.
    Type: Grant
    Filed: June 3, 1999
    Date of Patent: March 13, 2001
    Assignee: Human Genome Sciences, Inc.
    Inventors: Henrik S. Olsen, Timothy A. Coleman, Mark D. Adams
  • Guava (Psidium guajava) 13-hydroperoxide lyase and uses thereof
    Patent number: 6200794
    Abstract: The present invention relates to fatty acid 13-hydroperoxide lyase protein from guava (Psidium guajava) and the gene encoding the protein. Expression systems for recombinant guava 13-hydroperoxide lyase and methods of using recombinant guava 13-hydroperoxide lyase for the production of green notes are provided.
    Type: Grant
    Filed: May 13, 1998
    Date of Patent: March 13, 2001
    Assignees: Fimenrich SA, University of Zurich, Vanderbilt University
    Inventors: Ian Michael Whitehead, Alan John Slusarenko, Urs Wäspi, Duncan James Horatio Gaskin, Alan Richard Brash, Nathalie Tijet
  • Polypeptides having uroporphyrinogen decarboxylase activity and nucleic acids encoding same
    Patent number: 6200795
    Abstract: The present invention relates to isolated polypeptides having uroporphyrinogen decarboxylase activity and isolated nucleic acid sequences encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the nucleic acid sequences as well as methods for producing and using the polypeptides.
    Type: Grant
    Filed: November 16, 1998
    Date of Patent: March 13, 2001
    Assignee: Novo Nordisk Biotech, Inc.
    Inventor: Susan L. Elrod
  • Method for reducing absorption of dietary oxalate using enzymes and microbes
    Patent number: 6200562
    Abstract: This invention provides materials and procedures for the delivery of selected strains of bacteria and/or oxalate-degrading enzymes to the intestinal tracts of persons who are at increased risk for oxalate related disease because they have lost, or have inadequate concentrations of these bacteria. The administration of these bacteria and/or the relevant enzyme removes oxalate from the intestinal tract and thus reduces the amount of oxalate available for absorption and reduces the risk for oxalate related disease.
    Type: Grant
    Filed: May 22, 1998
    Date of Patent: March 13, 2001
    Assignee: Ixion Biotechnology, Inc.
    Inventors: Milton J. Allison, Harmeet Sidhu
  • Method and compositions for disrupting the epithelial barrier function
    Patent number: 6190894
    Abstract: A method for disrupting epithelial barrier function in a host in need of the topical administration of a physiologically active substance which comprises applying to the epithelium of the host, barrier-disrupting amount of at least one agent selected from the group consisting of an inhibitor of ceramide synthesis, inhibitor of acylceramide synthesis, inhibitor of glucosylceramide synthesis, and inhibitor of sphingomyelin synthesis, an inhibitor of fatty acid synthesis, an inhibitor of cholesterol synthesis, a degradation enzyme of ceramides, acylceramide, glucosylceramides, sphingomyelin, an inhibitor of phospholipid, glycosphingolipid, including glucosylceramide, acylceramide or sphingomyelin degradation, and both inhibitors and stimulators of metabolic enzymes of free fatty acids, ceramide, and cholesterol, as well as a topical composition useful therefore are disclosed.
    Type: Grant
    Filed: April 9, 1998
    Date of Patent: February 20, 2001
    Assignees: The Regents of the University of California, Cellegy Pharmaceuticals, Inc.
    Inventors: Carl R. Thornfeldt, Peter M. Elias, Kenneth R. Feingold, Walter M. Holleran
  • Pectate lyases
    Patent number: 6187580
    Abstract: A novel group of pectate lyases comprising the amino acid sequence Asn Leu Asn Ser Arg Val Pro (NLNSRVP) belonging to Family 1 of polysaccharide lyases have good performance in industrial processes under neutral or alkaline conditions such as laundering and textile processing. The pectate lyase are derivable from Bacillus species.
    Type: Grant
    Filed: November 24, 1998
    Date of Patent: February 13, 2001
    Assignee: Novo Nordisk A/S
    Inventors: Lene Nonboe Andersen, Martin Schülein, Niels Erik Krebs Lange, Mads Eskelund Bjørnvad, Søren Møller, Sanne O. Schrøder Glad, Markus Sakari Kauppinen, Kirk Schnorr, Lars Kongsbak
  • Polynucleotides encoding human sphingosine Lyase
    Patent number: 6187562
    Abstract: SPHINGLY polypeptides and polynucleotides and methods for producing such polypeptides by recombinant techniques are disclosed. Also disclosed are methods for utilizing SPHINGLY polypeptides and polynucleotides in therapy, and diagnostic assays for such.
    Type: Grant
    Filed: January 27, 1999
    Date of Patent: February 13, 2001
    Assignee: SmithKline Beecham plc
    Inventors: David Malcolm Duckworth, Robert James Godden, Tania Tamson Testa
  • Chondroitinase, process for preparing the same, and pharmaceutical composition comprising the same
    Patent number: 6184023
    Abstract: A crystallizable, purified chondroitinase ABC having a molecular weight of about 100,000 dalton by the measurement of the SDS-polyacrylamide gel electrophoresis (SDS-PAGE) and the measurement by the gel permeation chromatography method, having alanine as the N-terminal amino acid and proline as the C-terminal amino acid. A process for the purification of the crystallizable purified chondroitinase ABC comprising removing nucleic acid from an surfactant solution extract obtained from cells of chondroitinase ABC-producing microorganisms and chromatographically treating by concentration gradient elution using a weak cation exchange resin or a strong cation exchange resin. A composition comprising a chondroitinase and serum albumin, gelatin, or a nonionic surfactant.
    Type: Grant
    Filed: July 7, 1997
    Date of Patent: February 6, 2001
    Assignee: Seikgaka Corporation
    Inventors: Nobukazu Hashimoto, Hideo Mochizuki, Akio Hamai
  • Protein having ethylenediamine-N,N?-disuccinic acid:ethylenediamine lyase acitivity and gene encoding the same
    Patent number: 6168940
    Abstract: The present specification relates to a DNA comprising a nucleotide sequence coding for a polypeptide having ethylenediamine-N,N′-disuccinic acid:ethylenediamine lyase activity, the polypeptide having the amino acid sequence of SEQ ID NO: 1 which may have a deletion, substitution or addition of at least one amino acid; a recombinant plasmid containing the DNA; a host transformed with the recombinant plasmid; and a method for producing a diaminoalkylene-N,N′-disuccinic acid using the transformed host. The present specification also relates to a polypeptide having ethylenediamine-N,N′-disuccinic acid:ethylenediamine lyase activity which has the amino acid sequence of SEQ ID NO: 1 that may have a deletion, substitution or addition of at least one amino acid.
    Type: Grant
    Filed: December 1, 1997
    Date of Patent: January 2, 2001
    Assignee: Nitto Chemical Industry Co., Ltd.
    Inventor: Wataru Mizunashi
  • Expression in yeast of antigenically active, recombinant hybrid glutamic acid decarboxylase
    Patent number: 6165738
    Abstract: An antigenically active hybrid glutamic acid decarboxylase (GAD) comprising an amino-terminal moiety derived from the GAD67 isoform linked directly or indirectly with a middle and carboxy-terminal moiety derived from the GAD65 isoform, and production thereof as a recombinant protein by expression in eukaryotic host cells, particularly yeasts.
    Type: Grant
    Filed: September 13, 1999
    Date of Patent: December 26, 2000
    Assignees: Montech Medical Developments Pty. Ltd., Rondole Pty. Ltd.
    Inventors: Ian Reay Mackay, Merrill Joy Rowley, Paul Zev Zimmet, Brian Corner, Ruby Law, Khay-Lin Teoh
  • Pectin degrading enzymes from Bacillus licheniformis
    Patent number: 6165769
    Abstract: Pectin degrading enzymes derived from or endogeneous to Bacillus licheniformis or other Bacillus species which are at least 99% homologous to Bacillus licheniformis based on aligned 16S rDNA sequences have optimum activity at pH higher than 8. The pectin degrading enzymes belongs to the enzyme classes pectate lyases (EC 4.2.2.2), pectin lyases (EC 4.2.2.10) and polygalacturonases (EC 3.2.1.15) and are useful in industrial processes under alkaline conditions such as in textile processing and as an active ingredient eg in laundry detergents and hard surface cleaning products.
    Type: Grant
    Filed: November 24, 1998
    Date of Patent: December 26, 2000
    Assignee: Novo Nordisk A/S
    Inventors: Lene Nonboe Andersen, Martin Schulein, Niels Erik Krebs Lange, Mads Eskelund Bj.o slashed.rnvad, Kirk Schnorr
  • OxlT sequence and its use
    Patent number: 6162632
    Abstract: A DNA sequence encoding a transport protein responsible for the physical movement of oxalate and formate across the plasma membrane is provided. The protein, OxlT, can be isolated from the gram negative anaerobe, Oxalobacter formigenes. The protein is part of the three protein oxalate/formate antiport exchange system that allows for conversion of extracellular oxalate to formate+CO.sub.2.The OxlT coding sequence can be used to provide a metabolic energy system to the cell. The system comprises the OxlT transport protein in combination with oxalylCoA decarboxylase and formyl-CoA transferase. Expression cassettes can be designed to express all or part of the system in a target host. Such energy generating systems can be used in industrial settings that utilize fermentation. The energy generating systems of the invention extend culture longevity and increase product yield.
    Type: Grant
    Filed: February 25, 1998
    Date of Patent: December 19, 2000
    Assignee: Johns Hopkins School of Medicine
    Inventors: Peter C. Maloney, Keietsu Abe, Zhong-Shi Ruan
  • Process for the preparation of N-acetylneuraminic acid
    Patent number: 6156544
    Abstract: A method for the preparation of N-acetyl-D-neuraminic acid (NANA) N-acetyl-D-glucosamine (NAG) is described.
    Type: Grant
    Filed: July 2, 1998
    Date of Patent: December 5, 2000
    Assignee: Glaxo Group Limited
    Inventors: Michael John Dawson, David Noble, Mahmoud Mahmoudian
  • Use of glycosaminoglycans degrading enzymes for management of airway associated diseases
    Patent number: 6153187
    Abstract: A method of managing a patient having an accumulation of mucoid, mucopurulent or purulent material containing glycosaminoglycans, the method comprising the step of administering at least one glycosaminoglycans degrading enzyme to the patient in an amount therapeutically effective to reduce at least one of the following: the visco-elasticity of the material, pathogens infectivity and inflammation. An article of manufacture comprising an inhaler including, as an active ingredient, at least one glycosaminoglycans degrading enzyme for generating aerosols including the enzyme for management a patient having an accumulation of mucoid, mucopurulent or purulent material containing glycosaminoglycans.
    Type: Grant
    Filed: March 25, 1998
    Date of Patent: November 28, 2000
    Assignee: Insight Strategy & Marketing Ltd.
    Inventor: Oron Yacoby-Zeevi
  • High specificity homocysteinases and genes therefor
    Patent number: 6140102
    Abstract: Novel enzymatic methods to determine the concentration of homocysteine in biological fluids are described. In a typical embodiment of the invention, the biological fluid sample is from a patient, and the methods of the invention are useful to assess risk for cardiovascular disease. The novel methods of the invention involve use of particular homocysteinase enzymes that permit the determination of homocysteine concentrations in biological samples without interference from the concentrations of cysteine and/or of methionine that are routinely present in such samples. There is also provided a diagnostic kit for use in determining the amount of homocysteine in a biological sample comprising (a) a homocysteinase having the aformentioned characteristics, and (b) at least one reagent capable of being used to determine the amount of product formed in the homocysteinase reaction.
    Type: Grant
    Filed: November 19, 1997
    Date of Patent: October 31, 2000
    Assignee: AntiCancer, Inc.
    Inventors: Yuying Tan, Marcin Lenz
  • Process for producing alkali metal [S,S]-ethylenediamine-n,n'-disuccinates
    Patent number: 6136573
    Abstract: A process for producing an [S,S]-ethylenediamine-N,N'-disuccinic acid alkali metal salt which comprises reacting fumaric acid with ethylenediamine in an aqueous medium in the presence of an ion of at least one metal selected from the group consisting of alkaline earth metals and transition metals by the action of ethylenediamine-disuccinic acid ethylenediamine lyase of microorganism origin, adding an alkali hydroxide to a reaction product mixture thereby to separate and recover the metal ion as an insoluble precipitate, and obtaining an [S,S]-ethylenediamine-N,N'-disuccinic acid alkali metal salt.
    Type: Grant
    Filed: December 17, 1999
    Date of Patent: October 24, 2000
    Assignee: Mitsubishi Rayon Co., Ltd.
    Inventors: Makoto Kaneko, Takakazu Endo, Toshihiko Fukuda, Mami Kato
  • Method for the recombinant production of 1,3-propanediol
    Patent number: 6136576
    Abstract: The present invention provides an improved method for the production of 1,3-propanediol from a variety of carbon sources in an organism capable of 1,3-propanediol production and comprising DNA encoding protein X of a microorganismal dehydratase or protein X in combination with at least one of protein 1, protein 2 and protein 3, which proteins are comparable to those encoded by orfY, orfX and orfW, respectively from a microorganismal dha regulon. The protein X may be isolated from a diol dehydratase or a glycerol dehydratase. The present invention also provides host cells comprising protein X that are capable of increased production of 1,3-propanediol.
    Type: Grant
    Filed: November 13, 1997
    Date of Patent: October 24, 2000
    Assignee: Genencor International, Inc.
    Inventors: Maria Diaz-Torres, Nigel S Dunn-Coleman, Matthew W. Chase, Donald Trimbur
  • Polypeptides and polypeptide subunits of a stereospecific nitrile hydratase enzyme
    Patent number: 6133421
    Abstract: 350 The present invention provides a nitrile hydratase nucleic acid fragment isolated from Pseudomonas putida which encodes a nitrile hydratase activity capable of catalyzing the hydrolysis of certain racemic nitriles to the corresponding R- or S-amides. Also provided are transformed microorganisms capable of the active expression of said nitrile hydratase activity. Additionally, the invention provides a transformant harboring the nitrile hydratase gene in conjunction with an amidase gene, both of which may be co-expressed producing active nitrile hydratase and amidase enzymes respectively. Methods for the production of such enantiomeric materials are also provided.
    Type: Grant
    Filed: June 24, 1998
    Date of Patent: October 17, 2000
    Assignee: E. I. du Pont de Nemours & Company
    Inventors: Robert Donald Fallon, Mark James Nelson, Mark Scott Payne
  • Truncated aspartase enzyme derivatives and uses thereof
    Patent number: 6133013
    Abstract: Truncated derivatives of aspartase having enhanced catalytic activity and/or enhanced clot dissolution properties relative to native aspartase from E. coli. The enzymes are isolated by site-directed mutagenesis of DNA encoding aspartase. L-aspartic acid is manufactured by contacting fumarate and ammonium ion in the presence of an aspartase derivative.
    Type: Grant
    Filed: March 19, 1999
    Date of Patent: October 17, 2000
    Assignee: The University of Akron
    Inventors: Ronald E. Viola, Maithri M. K. Jayasekera, Abdullah S. Saribas
  • Pectate lyase
    Patent number: 6124127
    Abstract: The present invention relates to microbial pectate lyases, more specifically to microbial enzymes exhibiting pectate lyase activity as their major enzymatic activity in the neutral and alkaline pH ranges, to a method of producing such enzymes, and to methods for using such enzymes in the textile, detergent and cellulose fiber processing industries.
    Type: Grant
    Filed: May 6, 1998
    Date of Patent: September 26, 2000
    Assignee: Novo Nordisk A/S
    Inventors: Lene Nonboe Andersen, Martin Schulein, Niels Erik Krebs Lange
  • Genes for the biosynthesis of epothilones
    Patent number: 6121029
    Abstract: Nucleic acid molecules are isolated from Sorangium cellulosum that encode polypeptides necessary for the biosynthesis of epothilone. Disclosed are methods for the production of epothilone in recombinant hosts transformed with the genes of the invention. In this manner, epothilone can be produced in quantities large enough to enable their purification and use in pharmaceutical formulations such as those for the treatment of cancer.
    Type: Grant
    Filed: June 17, 1999
    Date of Patent: September 19, 2000
    Assignee: Novartis AG
    Inventors: Thomas Schupp, James Madison Ligon, Istvan Molnar, Ross Zirkle, Devon Dawn Cyr, Jorn Gorlach
  • Coniothyrium minitans xylanase gene Cxy1
    Patent number: 6121034
    Abstract: The nucleotide sequence of a novel xylanase gene obtained from the soilborne fungus Coniothyrium minitans, and denoted cxy1, is provided. The amino acid sequence of the encoded xylanase enzyme, denoted Cxy1, is also provided.
    Type: Grant
    Filed: May 13, 1999
    Date of Patent: September 19, 2000
    Assignee: Her Majesty the Queen in right of Canada, as represented by the Minister of Agriculture and Agri-Food
    Inventors: Andre J. Laroche, Timothy Y. Huang, Michele M. Frick, Zhen-Xiang Lu, Hung Chang Huang, Kuo Joan Cheng
  • Pyrrolnitrin biosynthesis genes and uses thereof
    Patent number: 6117670
    Abstract: The present invention is directed to the production of pyrrolnitrin in a host via recombinant expression of the polypeptides needed to biologically synthesize pyrrolnitrin. Genes isolated from P. fluorescens, P. pyrrocinia, B. cepacia, and M. fulvus that encode polypeptides necessary to produce pyrrolnitrin are provided, along with methods for identifying and isolating genes needed to recombinantly biosynthesize pyrrolnitrin from any organism capable of producing pyrrolnitrin. The isolated genes may be transformed and expressed in a desired host organisms to produce pyrrolnitrin according to the invention for a variety of purposes, including protecting the host from a pathogen, developing the host as a biocontrol agent, and producing large, uniform amounts of pyrrolnitrin.
    Type: Grant
    Filed: February 24, 1998
    Date of Patent: September 12, 2000
    Assignee: Novartis Finance Corporation
    Inventors: James Madison Ligon, Dwight Steven Hill, Stephen Ting Lam, Philip Eugene Hammer, Karl-Heinz van Pee, Sabine Kirner, Thomas R. Young
  • Compositions and methods for taxol biosynthesis
    Patent number: 6114160
    Abstract: The taxadiene synthase gene of Pacific yew has been cloned and its nucleic acid and polypeptide sequence is presented. Truncation or removal of the transit peptide increases expression of the cloned taxadiene synthase gene expression in E. coli cells.
    Type: Grant
    Filed: May 20, 1999
    Date of Patent: September 5, 2000
    Assignee: Washington State University Research Foundation
    Inventors: Rodney B. Croteau, Mark R. Wildung
  • F.sub.0 ATP synthase subunit
    Patent number: 6110722
    Abstract: The present invention provides a human ATP synthase subunit (ASYS) and polynucleotides which encode ASYS. The invention also provides expression vectors, host cells, agonists, antisense molecules, antibodies, or antagonists. The invention also provides methods for producing ASYS and for treating disorders associated with expression of ASYS.
    Type: Grant
    Filed: April 24, 1998
    Date of Patent: August 29, 2000
    Assignee: Incyte Pharmaceuticals, Inc.
    Inventors: Jennifer L. Hillman, Surya K. Goli
  • Soluble mammalian adenylyl cyclase and uses therefor
    Patent number: 6107076
    Abstract: A soluble form of adenylyl cyclase and methods of its use in screening for stimulators and inhibitors of adenylyl cyclase activity are disclosed. In one embodiment, a chimera of type I and type II adenylyl cyclases is provided. This chimera lacks transmembrane domains characteristic of adenylyl cyclases, rendering the recombinant product soluble, while retaining adenylyl cyclase function.
    Type: Grant
    Filed: October 4, 1996
    Date of Patent: August 22, 2000
    Assignee: Board of Regents, The University of Texas System
    Inventors: Wei-Jen Tang, Alfred G. Gilman
  • Method of forming ubiquinone-10
    Patent number: 6103488
    Abstract: The present invention relates to production of ubiquinone-10 in E. coli by isolating and sequencing a structural gene dds1 of decaprenyl diphosphate synthase derived from photosynthetic bacteria such as Rhodobacter capsulatus., and expressing it in E. coli.
    Type: Grant
    Filed: January 21, 1998
    Date of Patent: August 15, 2000
    Assignee: Alpha Foods Co., Ltd.
    Inventors: Hideyuki Matsuda, Makoto Kawamukai, Tsuyoshi Nakagawa, Katsunori Tanaka
  • Chondroitin lyase enzymes
    Patent number: 6093563
    Abstract: The present invention describes a method for the production of two highly purified enzymes capable of degrading chondroitin sulfate polysaccharides. A multi-step purification method incorporating cell disruption, cation exchange chromatography, affinity chromatography, hydroxylapatite chromatography, high resolution ion exchange chromatography and size exclusion is outlined. A 77,000.+-.5,000 Dalton protein capable of degrading chondroitin sulfates A and C and a 55,000.+-.2,300 Dalton protein capable of degrading dermatan sulfate were isolated. The genes encoding these enzymes, chondroitinase AC and chondroitinase B, respectively, have been cloned and methods for their use are described.
    Type: Grant
    Filed: July 8, 1994
    Date of Patent: July 25, 2000
    Assignee: IBEX Technologies R and D, Inc.
    Inventors: D. Clark Bennett, Maryse Laliberte, Kangfu Gu, Joseph Zimmermann, Anna Lydia Tkalec, Dominique Fink, Robert Linhardt
  • Fermentative carotenoid production
    Patent number: 6087152
    Abstract: Novel proteins of Flavobacterium sp. R1534 and the DNA sequences which encode these proteins are disclosed which provide an improved biosynthetic pathway from farnesyl pyrophosphate and isopentyl pyrophosphate to various carotenoid precursors and carotenoids, especially .beta.-carotene, lycopene, zeaxanthin and cantaxanthin.
    Type: Grant
    Filed: June 7, 1996
    Date of Patent: July 11, 2000
    Assignee: Roche Vitamins Inc.
    Inventors: Hans-Peter Hohmann, Luis Pasamontes, Michel Tessier, Adolphus van Loon
  • Method for synthesizing 2-ketoaldonic acids
    Patent number: 6080563
    Abstract: 2-Ketoaldonic acid is synthesized by aldolase condensation reaction involving pyruvate and an aldose acceptor in the presence of excess pyruvate. After the reaction is substantially complete, the excess pyruvate is removed from the reaction mixture by treatment with pyruvate decarboxylase.
    Type: Grant
    Filed: June 2, 1998
    Date of Patent: June 27, 2000
    Assignee: The Scripps Research Institute
    Inventor: Chi-Huey Wong
  • Therapeutic agent for herniated intervertebral disc
    Patent number: 6063378
    Abstract: A pharmaceutical composition and an agent that can be used for treatment of epidurally migrating herniated intervertebral disc are provided. The composition comprises a glycosaminoglycan degrading enzyme, preferably chondroitinase, more preferably chondroitinase ABC, in an amount effective to dissolve nucleus pulposus epidurally existing.
    Type: Grant
    Filed: August 20, 1998
    Date of Patent: May 16, 2000
    Assignee: Seikagaku Corporation
    Inventors: Yutaka Nohara, Hiroki Ishikawa
  • Chondroitin lyase enzymes
    Patent number: 6054569
    Abstract: The present invention describes a method for the production of two highly purified enzymes capable of degrading chondroitin sulfate polysaccharides. A multi-step purification method incorporating cell disruption, cation exchange chromatography, affinity chromatography, hydroxylapatite chromatography, high resolution ion exchange chromatography and size exclusion is outlined. A 77,000.+-.5,000 Dalton protein capable of degrading chondroitin sulfates A and C and a 55,000.+-.2,300 Dalton protein capable of degrading dermatan sulfate were isolated. The genes encoding these enzymes, chondroitinase AC and chondroitinase B, respectively, have been cloned and methods for their use are described.
    Type: Grant
    Filed: September 17, 1997
    Date of Patent: April 25, 2000
    Assignee: IBEX Technologies R and D, Inc.
    Inventors: D. Clark Bennett, Maryse Laliberte, Kangfu Gu, Joseph Zimmermann, Anna Lydia Tkalec, Dominique Fink, Robert Linhardt
  • (S)-hydroxynitrilelyase from Hevea brasiliensis
    Patent number: 6046042
    Abstract: A purified (S)-hydroxy-nitrile-lyase is disclosed. Also disclosed is an amino acid sequence for (S)-hydroxy-nitrile-lyase and DNA sequence encoding the same.
    Type: Grant
    Filed: December 22, 1997
    Date of Patent: April 4, 2000
    Assignee: DSM Chemie Linz GmbH
    Inventors: Meinhard Hasslacher, Michael Schall, Helmut Schwab, Elfriede Marianne Hayn, Sepp Kohlwein, Herfried Griengl
  • Gene encoding 2,3-dihydroxybenzoic acid decarboxylase
    Patent number: 6043076
    Abstract: The enzyme 2,3-dihydroxybenzoic acid decarboxylase has industrial applications for the decarboxylation of ring mounted carboxyls, specifically the decarboxylation of 2,3-dihydroxybenzoic acid to catechol. This invention relates to the isolation of a nucleic acid sequence from Aspergillus niger that encodes an enzyme that decarboxylates 2,3-dihydroxybenzoic acid. This invention further discloses the nucleic acid sequence, the protein sequence, vectors comprising the nucleic acid sequence, cells transformed with the nucleic acid sequence, and methods for the production of 2,3-dihydroxybenzoic acid decarboxylase.
    Type: Grant
    Filed: August 18, 1998
    Date of Patent: March 28, 2000
    Assignee: Board of Regents of University of Nebraska
    Inventor: Santha Ramakrishnan
  • Process for producing amide compound
    Patent number: 6043061
    Abstract: A process for producing an amide compound is described, wherein from a nitrile compound, the corresponding amide compound is produced by the action of nitrile hydratase, characterized in that the hydrocyanic acid concentration in a composition containing the nitrile compound is reduced by a chemical process, and thereafter nitrile hydratase acts on the nitrile compound. According to the invention, the decrease of the activity of the enzyme nitrile hydratase can be effectively suppressed so that an amide compound can be effectively produced from a nitrile compound.
    Type: Grant
    Filed: October 22, 1998
    Date of Patent: March 28, 2000
    Assignee: Mitsubishi Rayon Co., Ltd.
    Inventors: Katsuo Ishii, Kouzo Murao
  • Human N-acetylneuraminate lyase
    Patent number: 6030824
    Abstract: The invention provides a human N-acetylneuraminate lyase (HNANL) and polynucleotides which identify and encode HNANL. The invention also provides expression vectors, host cells, antibodies, agonists, and antagonists. The invention also provides methods for treating or preventing disorders associated with expression of HNANL.
    Type: Grant
    Filed: February 1, 1999
    Date of Patent: February 29, 2000
    Assignee: Incyte Pharmaceuticals
    Inventors: Jennifer L. Hillman, Purvi Shah, Neil C. Corley