Lyase (4. ) Patents (Class 435/232)
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Patent number: 8354262Abstract: The present disclosure relates to chemically modified carbonic anhydrase polypeptides and soluble compositions, homogenous liquid formulations comprising them. The chemically modified carbonic anhydrase polypeptides have improved properties relative to the same carbonic anhydrase polypeptide that is not chemically modified including the improved properties of increased activity and/or stability in the presence of amine compounds, ammonia, or carbonate ion. The present disclosure also provides methods of preparing the chemically modified polypeptides and methods of using the chemically modified polypeptides for accelerating the absorption of carbon dioxide from a gas stream into a solution as well as for the release of the absorbed carbon dioxide for further treatment and/or sequestering.Type: GrantFiled: June 30, 2011Date of Patent: January 15, 2013Assignee: Codexis, Inc.Inventors: Scott Novick, Oscar Alvizo
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Patent number: 8354248Abstract: A DNA fragment containing a gene which encodes a specific gene regulatory region alone or the gene regulatory region together with a signal peptide; a recombinant vector containing the DNA fragment; a transformant containing the recombinant vector; and a method of producing a recombinant protein by using the transformant. According to the invention, it is possible to produce a protein in a large amount at a high efficiency regardless of the kind of the recombinant protein.Type: GrantFiled: July 9, 2008Date of Patent: January 15, 2013Assignee: Japan Agency for Marine-Earth Science and TechnologyInventors: Yuji Hatada, Yukari Ohta, Yuko Hidaka, Nobuyuki Nakamura
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Publication number: 20130012424Abstract: The present invention relates to pectate lyase variants exhibiting alterations relative to a parent enzyme exhibiting pectate lyase activity as its major enzymatic activity; to a method of producing such enzymes; and to methods for using such enzymes in the textile, detergent and cellulose fiber processing industries. Compared to the parent enzyme, the pectate lyase variants of the present invention exhibit improved stability in detergents.Type: ApplicationFiled: September 6, 2012Publication date: January 10, 2013Applicant: NOVOZYMES A/SInventors: Sanne S. Glad, Carsten Andersen, Torben V. Borchert, Katja S. Johansen, Henrik Frisner, Thomas Thisted, Mads E. Bjørnvad
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Patent number: 8338158Abstract: Cis-aconitate decarboxylase mutants having one or more mutations in a C-terminal region as compared with a wild-type cis-aconitate decarboxylase of Aspergillus terreus.Type: GrantFiled: November 24, 2009Date of Patent: December 25, 2012Assignee: Industrial Technology Research InstituteInventors: Hsin-Ju Hsieh, Pei-Ching Chang, Kelly Teng
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Publication number: 20120322733Abstract: The present patent application concerns an enzyme capable of catalysing the conversion of a ?-hydroxyglycine to an ?-amide and the use of such enzymes for producing a C-terminal ?-amidated peptide.Type: ApplicationFiled: December 1, 2010Publication date: December 20, 2012Applicant: Novo Nordisk A/SInventor: Allan C. Shaw
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Publication number: 20120308547Abstract: One aspect of the present invention relates to mutants of chondroitinase ABCI. Such chondroitinase ABCI mutants exhibit altered chondroitin lyase activity or increased resistance to inactivation from stressors including exposure to UV light or heat. Methods of using chondroitinase ABCI mutant enzymes are also provided.Type: ApplicationFiled: July 6, 2012Publication date: December 6, 2012Inventors: Anthony O. CAGGIANO, Jennifer IACI, Andrea VECCHIONE, Elizabeth MARKENSOHN
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Publication number: 20120301947Abstract: A system for carbon fixation is provided. The system comprises enzymes which catalyze reactions of a carbon fixation pathway, wherein at least one of the reactions of the carbon fixation pathway is a carboxylation reaction, wherein products of the reactions of the carbon fixation pathway comprise oxaloacetate and malonyl-CoA, wherein an enzyme which performs the carboxylation reaction is selected from the group consisting of phophoenolpyruvate (PEP) carboxlase, pyruvate carboxylase and acetyl-CoA carboxylase and wherein an export product of the carbon fixation pathway is glyoxylate. Additional carbon fixation pathways are also provided and methods of generating same.Type: ApplicationFiled: February 10, 2011Publication date: November 29, 2012Applicant: Yeda Research and Development Co. Ltd.Inventors: Ron Milo, Arren Bar-Even, Elad Noor
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Patent number: 8309078Abstract: A polypeptide molecule able to selectively modulate uric acid conversion into S(+)- allantoin is described. A pharmaceutical composition for treating uric acid related disorders and a process to selectively modulate uric acid conversion into S(+)-allantoin are also disclosed.Type: GrantFiled: November 7, 2006Date of Patent: November 13, 2012Assignee: Università degli Studi di ParmaInventors: Riccardo Percudani, Claudia Folli, Ileana Ramazzina
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Publication number: 20120276567Abstract: The invention relates to a Talaromyces transformant comprising one or more recombinant gene, capable of producing cellulase in the absence of cellulase inducer in a glucose medium, having a cellulase activity of 2 WSU/ml or more, in 16 times or more diluted supernatant or broth.Type: ApplicationFiled: November 4, 2010Publication date: November 1, 2012Applicant: DSM IP ASSETS B.V.Inventors: Alrik Pieter Los, Brenda Vonk, Marco Alexander Berg Van Den, Robbertus Antonius Damveld, Cornelis Maria Jacobus Sagt, Adrianus Wilhelmus Hermanus Vollebregt, Margot Elisabeth Francoise Schooneveld-Bergmans
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Patent number: 8298529Abstract: Demethylation of a methylated DNA sequence in a eukaryotic cell is described, utilizing a molecule that includes at least a first domain that exhibits a cytidine deaminase activity and at least a second domain that confers either a specific or non-specific DNA binding activity. The molecules of the invention are useful in somatic cell nuclear transfer and also in cancer therapy.Type: GrantFiled: April 10, 2007Date of Patent: October 30, 2012Assignee: CellCentric LimitedInventors: Wolf Reik, Hugh Morgan, Chun-Fung Chan
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Publication number: 20120266329Abstract: The invention provides polypeptides, including enzymes, structural proteins and binding proteins, polynucleotides encoding these polypeptides, and methods of making and using these polynucleotides and polypeptides. Polypeptides, including enzymes and antibodies, and nucleic acids of the invention can be used in industrial, experimental, food and feed processing, nutritional and pharmaceutical applications, e.g., for food and feed supplements, colorants, neutraceuticals, cosmetic and pharmaceutical needs.Type: ApplicationFiled: February 20, 2012Publication date: October 18, 2012Applicant: BP Corporation North America Inc.Inventors: Eric J. Mathur, Cathy Chang
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Publication number: 20120263700Abstract: Human cystathionine ?-synthase variants are disclosed, as well as a method to produce recombinant human cystathionine ?-synthase and variants thereof. More particularly, the role of both the N-terminal and C-terminal regions of human CBS has been studied, and a variety of truncation mutants and modified CBS homologues are described. In addition, a method to express and purify recombinant human cystathionine ?-synthase (CBS) and variants thereof which have only one or two additional amino acid residues at the N-terminus are described.Type: ApplicationFiled: August 30, 2011Publication date: October 18, 2012Applicant: THE REGENTS OF THE UNIVERSITY OF COLORADOInventors: Jan P. Kraus, Jana Oliveriusova
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Publication number: 20120263719Abstract: The present invention provides monoclonal antibodies specific for members of the Aspergillus genus and uses thereof for increasing the survival rate of a subject infected with an Aspergillus species. The present invention also provides conjugates of alliinase with monoclonal antibodies specific for members of the Aspergillus genus that preserve the ability of the antibody to recognize the fungus as well as the activity of the enzyme to produce cytotoxic molecules of allicin from the substrate alliin. The invention further provides pharmaceutical compositions comprising the conjugates of the mAbs of the invention and alliinase and uses thereof in combination with the substrate alliin in the treatment of diseases cause by Aspergillus species, in particular invasive aspergillosis.Type: ApplicationFiled: October 21, 2010Publication date: October 18, 2012Applicant: YEDA RESEARCH AND DEVELOPMENT CO., LTD.Inventors: David Mirelman, Aharon Rabinkov, Elena Appel
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Publication number: 20120264169Abstract: The present invention relates to cells producing at least one polypeptide of interest and expressing one or more recombinant nuclease encoding gene(s) thereby producing the nuclease(s), and methods for producing a polypeptide of interest essentially free from contaminating DNA, said method comprising the steps of: (a) cultivating a cell that produces at least one polypeptide of interest and expresses one or more recombinant nuclease encoding gene(s) thereby producing the nuclease(s); and (b) isolating the polypeptide of interest.Type: ApplicationFiled: June 25, 2012Publication date: October 18, 2012Applicant: NOVOZYMES A/SInventors: Michael Dolberg Rasmussen, Jon Martin Persson
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Patent number: 8288144Abstract: The present invention relates to pectate lyase variants exhibiting alterations relative to a parent enzyme exhibiting pectate lyase activity as its major enzymatic activity; to a method of producing such enzymes; and to methods for using such enzymes in the textile, detergent and cellulose fiber processing industries. Compared to the parent enzyme, the pectate lyase variants of the present invention exhibit improved stability in detergents.Type: GrantFiled: June 10, 2009Date of Patent: October 16, 2012Assignee: Novozymes A/SInventors: Sanne Schroder Glad, Carsten Andersen, Torben Vedel Borchert, Katja Salomon Johansen, Henrik Frisner, Mads Eskelund Bjornvad, Thomas Thisted
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Patent number: 8288147Abstract: Methods for synthesizing isopentenyl pyrophosphate are provided. A first method comprises introducing into a host microorganism a plurality of heterologous nucleic acid sequences, each coding for a different enzyme in the mevalonate pathway for producing isopentenyl pyrophosphate. A related method comprises introducing into a host microorganism an intermediate in the mevalonate pathway and at least one heterologous nucleic acid sequence, each sequence coding for an enzyme in the mevalonate pathway necessary for converting the intermediate into isopentenyl pyrophosphate. The invention also provides nucleic acid sequences, enzymes, expression vectors, and transformed host cells for carrying out the methods.Type: GrantFiled: February 15, 2011Date of Patent: October 16, 2012Assignee: The Regents of the University of CaliforniaInventors: Jay D. Keasling, Vincent J.J. Martin, Douglas J. Pitera, Seon-Won Kim, Sydnor T. Withers, III, Yasuo Yoshikuni, Jack Newman, Artem Valentinovich Khlebnikov
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Patent number: 8288132Abstract: The present invention discloses a chemoenzymatic process for the preparation of an iminocyclitol corresponding to formula (I), (II), (III) or (IV), wherein: R1 and R2 are the same or different, and independently selected from the group consisting of: H, OH, hydroxymethyl, methyl, ethyl, butyl, pentyl, hexyl, octyl, isopropyl, isobutyl, 2-methylbutyl, and benzyl; R3 is selected from the group consisting of: H, hydroxymethyl, hydroxyethyl, ethyl, butyl, pentyl, hexyl, octyl, dodecyl, isobutyl, isopropyl, isopentyl, 2-methylbutyl, benzyl, and phenylethyl; n: 0 or 1; the process comprising: (i) an aldol addition catalyzed by a D-fructose-6-phosphate aldolase enzyme (FSA) and an acceptor aminoaldehyde; and (ii) an intramolecular reductive amination of the addition adduct obtained in step (i) with H2, in the presence of a metallic catalyst, optionally being carried out said step (ii) in the presence of an aldehyde of formula R3—CHO, wherein R3 is as defined above.Type: GrantFiled: August 30, 2007Date of Patent: October 16, 2012Assignees: Consejo Superior de Investigaciones Cientificas, Bioglane, S.L.N.E.Inventors: Pere Clapés Saborit, Jesús Joglar Tamargo, José Antonio Castillo Expósito, Carles Lozano Pérez
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Patent number: 8283146Abstract: The invention is related to processing enzyme comprising an N-terminally attached tag derived from highly basic proteins from thermophilic bacteria. The processing enzymes are useful for modifying proteins. They can be produced in high yields and can be effectively separated from the modified protein after use.Type: GrantFiled: May 17, 2012Date of Patent: October 9, 2012Assignee: Novo Nordisk Health Care AGInventor: Allan Christian Shaw
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Patent number: 8273556Abstract: 4-(Indol-3-ylmethyl)-4-hydroxy-2-oxoglutarate, which is useful as an intermediate in the synthesis of monatin, may be synthesized from indole pyruvic acid and pyruvic acid (and/or oxaloacetic acid) by using a novel aldolase derived from the genus Pseudomonas, Erwinia, Flavobacterium, or Xanthomonas.Type: GrantFiled: September 30, 2011Date of Patent: September 25, 2012Assignee: Ajinomoto Co., Inc.Inventors: Masakazu Sugiyama, Kunihiko Watanabe, Shigeru Kawahara
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Patent number: 8273950Abstract: Polynucleotides encoding polypeptides that comprise the biosynthetic pathway for phenylpropanoids and flavonoids in the coffee plant are disclosed. Also disclosed are methods for using these polynucleotides and polypeptides for the manipulation of flavor, aroma, and other features of coffee beans, as well as the manipulation resistance to pathogen, herbivore, and insect attack in the coffee plant.Type: GrantFiled: October 13, 2006Date of Patent: September 25, 2012Assignees: Nestec S. A., Cornell UniversityInventors: Maud Lepelley, Gerald Cheminade, James Gérard McCarthy, Vincent Petiard, Chenwei Lin, Steven D. Tanksley
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Publication number: 20120231498Abstract: The invention is related to processing enzyme comprising an N-terminally attached tag derived from highly basic proteins from thermophilic bacteria. The processing enzymes are useful for modifying proteins. They can be produced in high yields and can be effectively separated from the modified protein after use.Type: ApplicationFiled: May 17, 2012Publication date: September 13, 2012Applicant: Novo Nordisk Health Care AGInventor: Allan Christian Shaw
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Patent number: 8263383Abstract: The present invention relates to polynucleotide and polypeptide sequences of novel carbonic anhydrase variants having increased stability under high temperature conditions compared to native carbonic anhydrase.Type: GrantFiled: March 6, 2009Date of Patent: September 11, 2012Assignee: CO2 Solutions, Inc.Inventors: Richard Daigle, Marc Desrochers
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Publication number: 20120220002Abstract: The present disclosure provides engineered halohydrin dehalogenase (HHDH) polypeptides having improved enzyme properties as compared to the wild-type HHDH enzyme HheC and other reference engineered HHDH polypeptides. Also provided are polynucleotides encoding the engineered HHDH enzymes, host cells capable of expressing the engineered HHDH enzymes, and methods of using the engineered HHDH enzymes to synthesize a variety of chiral compounds including chiral epoxides and chiral alcohols.Type: ApplicationFiled: April 20, 2012Publication date: August 30, 2012Applicant: CODEXIS, INC.Inventors: Erik De Vries, Louis Clark, Scott McVicar, Erika Segraves, Shiwei Song, Kheng Lin Tan
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Publication number: 20120220016Abstract: To provide a pectin lyase and a gene for a pectin lyase which can be used in an enzyme preparation for processing plant tissue, and which possesses sufficient maceration activity even under highly acidic conditions. Isolation and purification of a pectin lyase and a gene for a pectin lyase having a molecular weight of 39,500 produced by an Aspergillus filamentous fungus, and possessing a maceration activity and a pectin lyase activity. The pectin lyase has a specified amino acid sequence, an optimal pH of 3.5 for maceration activity, an optimal pH of 4.5 for pectin lyase activity, and deactivates with boiling treatment for 15 minutes at 121° C. Sterilizing heat treatment are unnecessary, because the present invention possesses sufficient maceration activity at pH 3.0-4.0, and the resulting pectin lysate possesses a bactericidal activity and a potent antibacterial activity.Type: ApplicationFiled: July 15, 2010Publication date: August 30, 2012Inventors: Yoshihisa Nakano, Mitsuhiro Uenoyama, Erina Kobayashi
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Publication number: 20120213808Abstract: The purpose of the present invention is to provide a method for treating autoimmune diseases. Disclosed is a method or the like for the treatment of autoimmune diseases, which utilizes an antigen-specific tolerogenic antigen presenting cell. Specifically disclosed are: a method for producing an antigen-specific tolerogenic antigen presenting cell, which is characterized by using carbonic anhydrase I; an immunogenic antigen presenting cell which is specific to carbonic anhydrase I; and a method or the like for the treatment of autoimmune diseases (especially inflammatory bowel diseases), which is characterized by using a tolerogenic antigen presenting cell which is specific to carbonic anhydrase I.Type: ApplicationFiled: October 7, 2010Publication date: August 23, 2012Applicant: National University Corporation Ehime UniversityInventors: Hidehiro Murakami, Hirofumi Yamanishi, Morikazu Onji
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Patent number: 8247198Abstract: It has surprisingly been discovered that it is possible to use enzymes in deep eutectic solvents (DES). DES's are mixtures of a nitrogen salt or a metal salt and a strong hydrogen bond donor that can be mixed in proportions that form a eutectic point.Type: GrantFiled: September 22, 2008Date of Patent: August 21, 2012Assignee: Friedrich SriencInventors: Johnathan T. Gorke, Romas J. Kazlauskas, Friedrich Srienc
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Publication number: 20120208259Abstract: The present invention relates to novel halohydrin dehalogenase polypeptides and the polynucleotides that encode them. These polypeptides are useful in the production of 4-substituted-3-butyric acid derivatives and vicinal cyano, hydroxyl substituted carboxylic acid esters. The invention also provides related vectors, host cells and methods.Type: ApplicationFiled: January 12, 2012Publication date: August 16, 2012Applicant: CODEXIS, INC.Inventors: S. Christopher Davis, Richard John Fox, Gjalt W. Huisman, Vesna Gavrilovic, Lisa Marie Newman
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Publication number: 20120208173Abstract: An isolated nucleic acid molecule that encodes a terpene synthase and is selected from among: a) a nucleic acid molecule comprising the sequence of nucleotides set forth in SEQ ID NO: 1, SEQ ID NO: 3 or SEQ ID NO: 5; b) a nucleic acid molecule that is a fragment of (a); c) a nucleic acid molecule comprising a sequence of nucleotides that is complementary to (a)- or (b); and d) a nucleic acid molecule that encodes a terpene synthase having at least or at least about or at least about 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99% identity to any one of (a)-(c); wherein the nucleic acid molecule encodes a terpene synthase.Type: ApplicationFiled: June 25, 2010Publication date: August 16, 2012Inventors: Katherine Zulak, Christopher Jones, Jessie Moniodis, Joerg Bohlmann
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Publication number: 20120202262Abstract: The invention relates to a process for production of an enzyme product having a plurality of enzyme activities obtained by fermentation of an Aspergillus strain.Type: ApplicationFiled: December 21, 2009Publication date: August 9, 2012Applicant: Danisco A/SInventor: Mette Zacho Gronfeldt
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Patent number: 8236302Abstract: One aspect of the present invention relates to mutants of chondroitinase ABCI. Such chondroitinase ABCI mutants exhibit altered chondroitin lyase activity or increased resistance to inactivation from stressors including exposure to UV light or heat. Methods of using chondroitinase ABCI mutant enzymes are also provided.Type: GrantFiled: June 4, 2010Date of Patent: August 7, 2012Assignee: Acorda Therapeutics, Inc.Inventors: Anthony O. Caggiano, Jennifer Iaci, Andrea Vecchione, Elizabeth Markensohn
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Publication number: 20120189604Abstract: The present invention comprises methods and compositions for the reduction of oxalate in humans, and methods for the purification and isolation of recombinant oxalate reducing enzyme proteins. The invention provides methods and compositions for the delivery of oxalate-reducing enzymes in particle compositions. The compositions of the present invention are suitable in methods of treatment or prevention of oxalate-related conditions.Type: ApplicationFiled: July 1, 2010Publication date: July 26, 2012Inventors: Harmeet Sidhu, Qingshan Li, Aaron Blake Cowley, Carl-Gustaf Gölander
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Patent number: 8227227Abstract: The present invention relates to cells producing at least one polypeptide of interest and expressing one or more recombinant nuclease encoding gene(s) thereby producing the nuclease(s), and methods for producing a polypeptide of interest essentially free from contaminating DNA, said method comprising the steps of: (a) cultivating a cell that produces at least one polypeptide of interest and expresses one or more recombinant nuclease encoding gene(s) thereby producing the nuclease(s); and (b) isolating the polypeptide of interest.Type: GrantFiled: November 30, 2007Date of Patent: July 24, 2012Assignee: Novozymes A/SInventors: Michael Dolberg Rasmussen, Jon Martin Persson
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Publication number: 20120177722Abstract: This invention provides polypeptides having lyase activity, polynucleotides encoding these polypeptides, and meth-°ds of making and using these polynucleotides and polypeptides. In one aspect, the invention is directed to polypeptides having ammonia lyase activity, e.g., phenylalanine ammonia lyase, tyrosine ammonia lyase and/or histidine ammonia lyase activity, including thermostable and thermotolerant activity, and polynucleotides encoding these enzymes, and making and using these polynucleotides and polypeptides. The polypeptides of the invention can be used in a variety of pharmaceutical, agricultural and industrial contexts. X?NO2, Cl, Br, NH2, OH, H, alkyl at one or several o, m, and p positions R?H or alkyl.Type: ApplicationFiled: November 2, 2011Publication date: July 12, 2012Inventors: David P. Weiner, Alexander Varvak, Toby Richardson, Mircea Podar, Ellen Burke, Shaun Healey
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Publication number: 20120178637Abstract: Methods for classifying a test sample as indicative of Alzheimer's disease use protein and peptide biomarkers that are differentially expressed in the cerebral spinal fluid (CSF) of subjects with Alzheimer's disease relative to age-matched controls. The methods also use protein and peptide signatures indicative of Alzheimer's disease. Microarrays and kits for detecting the protein and peptide biomarkers in CSF samples can be used to classify Alzheimer's disease state from test samples.Type: ApplicationFiled: July 7, 2010Publication date: July 12, 2012Applicant: ABBOTT LABORATORIESInventors: Viswanath Devanarayan, Melanie Joy Patterson, Jeffrey F. Waring, David Witte
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Publication number: 20120171749Abstract: The present invention relates to methods for monitoring differential expression of a plurality of genes in a first Bacillus cell relative to expression of the same genes in one or more second Bacillus cells using microarrays containing Bacillus genomic sequenced tags. The present invention also relates to computer readable media and computer-based systems. The present invention further relates to substrates containing an array of Bacillus licheniformis or Bacillus clausii GSTs.Type: ApplicationFiled: March 15, 2012Publication date: July 5, 2012Applicants: Novozymes, Inc., Novozymes A/SInventors: Alexandre Bolotine, Alexei Sorokine, Alla Lapidus, Randy M. Berka, Ib Groth Clausen
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Publication number: 20120164146Abstract: An isolated peptide of 12-20 amino acids in length comprising the amino acid sequence SEQ ID NO:1, wherein the serine residue (S) at position 8 of SEQ ID NO:1 is phosphorylated, is provided. Also provided is a human monophosphorylated alpha-enolase isoform wherein the serine residue (S) at position 419 of the human alpha-enolase amino acid sequence (SEQ ID NO:2) is phosphorylated and in which other post-translational modifications may be present. Further provided are antibodies capable of specifically binding the peptide and/or the isoform of the invention. The peptide, the isoform and the antibodies of the invention may be used in the diagnosis and/or amelioration and/or treatment of pancreatic adenocarcinoma.Type: ApplicationFiled: September 9, 2010Publication date: June 28, 2012Applicants: NATIMAB THERAPEUTICS S.R.L.Inventors: Francesco Novelli, Barbara Tomaino, Paola Cappello
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Publication number: 20120159838Abstract: The present invention relates to a process for producing enzymes and single cell oil. The process comprises that microorganisms capable of producing both single cell oil and enzymes are cultivated under conditions suitable for single cell oil production and enzyme production in a single cell oil production process. A microorganism culture comprising single cell oil and enzymes is obtained and at least part of the microorganism culture, of the supernatant and/or microorganism cells separated from the microorganism culture, of protein fraction enriched from the supernatant, and/or of protein fraction obtained from the cells is used as an enzyme preparation or as a source of enzymes. Single cell oil is recovered from the microorganism cells and used as biofuel, component of biofuel or as a starting material for biofuel production. Enzymes produced according to the process are used in the same or in another industrial process.Type: ApplicationFiled: December 21, 2011Publication date: June 28, 2012Applicant: NESTE OIL OYJInventors: Annika Malm, Simo Laakso, Ossi Pastinen, Heidi Kahelin, Miia Mujunen
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Patent number: 8206959Abstract: The invention is related to processing enzyme comprising an N-terminally attached tag derived from highly basic proteins from thermophilic bacteria. The processing enzymes are useful for modifying proteins. They can be produced in high yields and can be effectively separated from the modified protein after use.Type: GrantFiled: November 11, 2010Date of Patent: June 26, 2012Assignee: Novo Nordisk Health Care AGInventor: Allan Christian Shaw
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Patent number: 8206970Abstract: Methods for the fermentive production of four carbon alcohols are provided. Specifically, butanol, preferably 2-butanol is produced by the fermentive growth of a recombinant bacteria expressing a 2-butanol biosynthetic pathway. The recombinant microorganisms and methods of the invention can also be adapted to produce 2-butanone, an intermediate in the 2-butanol biosynthetic pathways disclosed herein.Type: GrantFiled: April 30, 2007Date of Patent: June 26, 2012Assignee: Butamax(TM) Advanced Biofuels LLCInventors: Andrew C. Eliot, Vasantha Nagarajan, Lixuan Lisa Huang
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Patent number: 8198050Abstract: The invention provides recombinant B. thetaiotaomicron GAG lyase polypeptides. The invention also provides nucleic acid molecules encoding such polypeptides, recombinant expression vectors containing B. thetaiotaomicron GAG lyase nucleic acid molecules, and host cells into which the expression vectors have been introduced. Characterization, diagnostic and therapeutic methods utilizing compositions of the invention are also provided.Type: GrantFiled: December 21, 2010Date of Patent: June 12, 2012Assignee: Momenta Pharmaceuticals, Inc.Inventor: James Myette
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Patent number: 8198066Abstract: Methods and materials related to producing 3-HP as well as other organic compounds are disclosed. Specifically, isolated nucleic acids, polypeptides, host cells, and methods and materials for producing 3-HP and other organic compounds are disclosed.Type: GrantFiled: November 11, 2011Date of Patent: June 12, 2012Assignee: Cargill, IncorporatedInventors: Ravi R. Gokarn, Olga V. Selifonova, Holly Jean Jessen, Steven John Gort, Thorsten Selmer, Wolfgang Buckel
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Publication number: 20120142057Abstract: The present invention provides a method capable of producing a natural or recombinant protein in high yield. The present invention relates to a method of producing a polypeptide, comprising culturing a cell which strongly expresses cysteine sulfinic acid decarboxylase and has a transferred DNA encoding a desired polypeptide and thereby allowing the cell to produce the polypeptide. Hamster cysteine sulfinic acid decarboxylase, a DNA encoding the same, a recombinant vector and a transformed cell are also provided.Type: ApplicationFiled: February 8, 2012Publication date: June 7, 2012Inventors: Hisahiro Tabuchi, Satoshi Tainaka, Tomoya Sugiyama
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Publication number: 20120142077Abstract: Disclosed herein are techniques for computationally designing enzymes. These techniques can be used to design variations of naturally occurring enzymes, as well as new enzymes having no natural counterparts. The techniques are based on first identifying functional reactive sites required to promote the desired reaction. Then, hashing algorithms are used to identify potential protein backbone structures (i.e., scaffolds) capable of supporting the required functional sites. These techniques were used to design 32 different protein sequences that exhibited aldol reaction catalytic function, 31 of which are defined in the Sequence Listing. Details of these 31 different synthetic aldolases are provided, including descriptions of how such synthetic aldolases can be differentiated from naturally occurring aldolases.Type: ApplicationFiled: December 1, 2011Publication date: June 7, 2012Applicant: University of WashingtonInventors: David Baker, Alexandre Zanghellini, Lin Jiang, Andrew Wollacott, Daniela Grabs-Röthlisberger, Eric Althoff
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Patent number: 8187856Abstract: The present disclosure provides engineered halohydrin dehalogenase (HHDH) polypeptides having improved enzyme properties as compared to the wild-type HHDH enzyme HheC and other reference engineered HHDH polypeptides. Also provided are polynucleotides encoding the engineered HHDH enzymes, host cells capable of expressing the engineered HHDH enzymes, and methods of using the engineered HHDH enzymes to synthesize a variety of chiral compounds including chiral epoxides and chiral alcohols.Type: GrantFiled: December 18, 2009Date of Patent: May 29, 2012Assignee: Codexis, Inc.Inventors: Erik De Vries, Louis Clark, Scott McVicar, Erika Segraves, Shiwei Song, Kheng Lin Tan
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Patent number: 8187859Abstract: The invention provides yeast strains transformed to reduce nitrogen catabolite repression of a gene encoding a urea degrading enzymatic activity expressed by the yeast strain under fermenting conditions. Strains of Saccharomyces cerevisiae are for example provided having enhanced DUR1,2 urea carboxylase-allophanate hydrolase activity under wine fermenting conditions.Type: GrantFiled: November 6, 2002Date of Patent: May 29, 2012Assignee: The University of British ColumbiaInventors: Hendrik Jurgens Jansen Van Vuuren, Aline Lonvaud, Joana Coulon, Debra Inglis
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Patent number: 8183028Abstract: Compositions and methods for producing olefins are described herein. The olefins can be used to produced biofuels.Type: GrantFiled: December 22, 2008Date of Patent: May 22, 2012Assignee: LS9, Inc.Inventors: Murtaza Alibhai, Mathew Rude, Andreas Schirmer
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Publication number: 20120122166Abstract: The present invention concerns a method for the production of a biochemical selected among lactic acid, acetol and 1,2-propanediol, comprising culturing a microorganism modified for an improved production of the biochemical selected among lactic acid, acetol and 1,2-propanediol in an appropriate culture medium and recovery of the desired biochemical which may be further purified wherein the microorganism expresses a methylglyoxal synthase (MGS) enzyme which activity is not inhibited by orthophosphate. The present invention concerns a mutant methylglyoxal synthase (MGS) comprising at least one amino acid residue in the protein sequence of the parent enzyme replaced by a different amino acid residue at the same position wherein the mutant enzyme has retained more than 50% of the methylglyoxal synthase activity of the parent enzyme and the methylglyoxal synthase activity of the mutant MGS is not inhibited by orthophosphate as compared to the parent enzyme.Type: ApplicationFiled: July 30, 2010Publication date: May 17, 2012Applicant: METABOLIC EXPLORERInventors: Francois Voelker, Laurence Dumon-Seignovert, Philippe Soucaille
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Publication number: 20120122184Abstract: A new strain Bacillus sp. P203 (Bacillus plakortiensis) is disclosed. Isolated mature functional polypeptide which is obtainable from the bacterium strain Bacillus sp. P203 deposited under accession number DSM 17419 are disclosed.Type: ApplicationFiled: January 26, 2012Publication date: May 17, 2012Applicant: NOVOZYMES A/SInventors: Preben Nielsen, Reinhard Wilting, Martin Borchert
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Publication number: 20120115153Abstract: The present invention relates to a marker for the prognosis of liver cancer; a composition for estimating the prognosis of liver cancer, which contains a substance for detecting a change in the expression level of the prognostic marker for liver cancer; a kit for estimating the prognosis of liver cancer, which contains the composition for estimating liver cancer prognosis; a method for estimating the prognosis of liver cancer using the marker for liver cancer prognosis; and a method for screening a therapeutic agent for liver cancer using the marker for the prognosis of liver cancer.Type: ApplicationFiled: April 16, 2010Publication date: May 10, 2012Applicants: CBS BIOSCIENCE, CO., LTD.Inventors: Jin young Park, Seok joo Hong, Jong Min Kim
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Publication number: 20120115186Abstract: The present invention relates to a process for the production of one or more fermentation product from a sugar composition, comprising the following steps: a) fermentation of the sugar composition in the presence of a yeast belonging to the genera Saccharomyces, Kluyveromyces, Candida, Pichia, Schizosaccharomyces, Hansenula, Kloeckera, Schwanniomyces or Yarrowia, and b) recovery of the fermentation product, wherein the yeast comprises the genes araA, araB and araD and the sugar composition comprises glucose, galactose and arabinose.Type: ApplicationFiled: July 6, 2010Publication date: May 10, 2012Applicant: DSM IP ASSETS B.V.Inventors: Paul Klaassen, Gijsberdina Pieternella Van Suylekom, Bianca Elisabeth Maria Gielesen, Nicolette Jasmijn Broers, Beate Wiedemann, Wilhelmus Theodorus Antonius Maria De Laat