Lysis Of Micro-organism Patents (Class 435/259)
  • Patent number: 10072284
    Abstract: The present invention is generally directed to a lysis buffer for extraction of DNA from plant material and improved methods for extraction of DNA from plant material utilizing the novel lysis buffer. Advantageously, the lysis buffer of the present invention is suitable for use in connection with simpler analysis methods, while still providing suitable DNA yields and purities for analysis.
    Type: Grant
    Filed: June 20, 2013
    Date of Patent: September 11, 2018
    Assignee: Monsanto Technology LLC
    Inventors: Thomas R. Mertz, Jr., Saurabh Parikh, Julie Oermann, Tracey Hodge, Bryan Witherbee
  • Patent number: 10072258
    Abstract: A method for preparing a sample of biological materials containing target cells and accompanying cells for extracting nucleic acids of the target cells includes accumulating the target cells of the sample by separating the target cells or the accompanying cells from the sample. The method also includes digesting the target cells via chemical and/or physical lysis in order to produce a target cell lysate containing the nucleic acids of the target cells. The method furthermore includes purifying the nucleic acids from the target cell lysate in order to extract the nucleic acids of the target cells.
    Type: Grant
    Filed: July 30, 2014
    Date of Patent: September 11, 2018
    Assignee: Robert Bosch GmbH
    Inventors: Bernd Faltin, Franz Laermer, Jochen Rupp, Peter Rothacher, Christian Dorrer, Sebastian Berning
  • Patent number: 9951467
    Abstract: The present invention provides a method for imparting bacteriostatic effect to a textile product by immersing the textile product for 20 minutes or more in an aqueous solution containing a cationic polypeptide at a concentration of 250 ppm or more, which solution has a temperature of 25° C. or more. By bacteriostatically treating a textile product in accordance with such method, the bacteriostatic effect is not reduced and thus retained even after the textile product is washed for a plurality of times.
    Type: Grant
    Filed: December 17, 2010
    Date of Patent: April 24, 2018
    Assignee: JNC CORPORATION
    Inventors: Reiko Katoh, Naoyuki Yoshida, Masamichi Mutoh
  • Patent number: 9932624
    Abstract: A method for selectively recovering nucleic acid from a sperm cell in a sample containing cells of at least a sperm cell and an epithelial cell, and a cell suspension medium comprising extracellular impurities, is provided. The method entails introducing a sample into a vessel, sequestering the cells from the remaining sample components, washing the cells with a washing solution either before or after sequestration, removing the impurities-containing cell suspension medium from the vessel while retaining the cells; lysing selectively cells of the first cell type; and isolating the nucleic acid from the lysed cells. Methods for recovering nucleic acid from the second cell type are also provided.
    Type: Grant
    Filed: February 2, 2015
    Date of Patent: April 3, 2018
    Assignee: LIFE TECHNOLOGIES CORPORATION
    Inventor: Jason Yingjie Liu
  • Patent number: 9816085
    Abstract: A device configured for lysing a sample includes a holding element configured to hold an energy-transmitting element such that the holding element lies around the held part of the energy-transmitting element and envelops the held part of the energy-transmitting element and/or that the holding element lies against the held part of the energy-transmitting element. The holding element is further configured to cause lysis with the energy-transmitting element when the holding element is immersed into the sample to be subjected to lysis or comes into contact with the sample to be subjected to lysis. A method includes inserting the energy-transmitting element into the holding element. A system configured for lysing a sample by way of ultrasound includes the device and the energy transmitting element.
    Type: Grant
    Filed: October 24, 2013
    Date of Patent: November 14, 2017
    Assignee: Robert Bosch GmbH
    Inventors: Martina Daub, Jochen Rupp, Peter Rothacher
  • Patent number: 9783578
    Abstract: The present invention provides immunosuppression compounds to inhibit the programmed cell death 1 (PD1) signalling pathway. The present invention further provides peptide based compositions for treatment of cancer or treatment of infections via immunopotentiation caused by inhibition of immunosuppressive signaling induced by PD-1, PD-L1, or PD-L2 and therapies using them, immunopotentiative substrates included as the active ingredient. Further, the invention provides an application of the compositions containing the peptide moieties for preventive and/or therapeutic agents for cancer, cancer metastasis, immunodeficiency, an infectious disease or the like and an application of peptide moieties as a testing or diagnostic agent or a research agent for such a disease.
    Type: Grant
    Filed: December 8, 2014
    Date of Patent: October 10, 2017
    Assignee: AURIGENE DISCOVERY TECHNOLOGIES LIMITED
    Inventors: Pottayil Govindan Nair Sasikumar, Muralidhara Ramachandra, Suresh Kumar Vadlamani, Koteswara Rao Vemula, Leena Khare Satyam, Krishnaprasad Subbarao, K. Rajeev Shrimali, Sreenivas Kandepu
  • Patent number: 9758755
    Abstract: A cell lysis workflow involving a cell suspension that is passed through a filter in a first flow direction from a first side of the filter toward a second side thereof. The filter captures a plurality of cells on the first side. A lysis solution is passed through the filter in a second flow direction opposite the first direction, thereby dislodging the plurality of cells captured on the filter, and resuspending the plurality of cells in the lysis solution on the first side of the filter. The lysis solution lyses the plurality of cells to produce a cellular lysate. The cellular lysate is passed through the filter in the first flow direction.
    Type: Grant
    Filed: October 23, 2015
    Date of Patent: September 12, 2017
    Assignee: LIFE TECHNOLOGIES CORPORATION
    Inventor: Philip Martin
  • Patent number: 9556495
    Abstract: A method and apparatus for locating and selecting a colony of microorganisms on a culture dish and identifying microorganisms in said selected colony using MALDI. The method comprises the automated steps of locating and selecting a colony of microorganisms on a culture dish; obtaining a sample of said selected colony of microorganisms; depositing at least some of said sample of said selected colony of microorganisms on a target plate; and transferring said target plate with said sample in an apparatus for performing MALDI for identification of said sample of said selected colony of microorganisms. A sample of a colony of microorganisms is automatically deposited on a depositing spot such that the sample covers at most approximately half of said one of the depositing spots of the target plate.
    Type: Grant
    Filed: April 2, 2013
    Date of Patent: January 31, 2017
    Assignee: BD Kiestra B.V.
    Inventors: Jetze Botma, Martijn Kleefstra, Tino Walter Van Der Zee
  • Publication number: 20150125865
    Abstract: Methods and systems are provided for merging a droplet with a volume of fluid in a microfluidic system. In particular, the methods of the invention use a microfluidic structure designed to merge a fluid with a droplet in order to dilute, add volume, or add selected reagents, biological materials, or synthetic materials to a droplet. Also provided are related systems and methods for cell lysis.
    Type: Application
    Filed: December 20, 2012
    Publication date: May 7, 2015
    Inventors: David Scott Johnson, Gary Withey, Philip Homewood, Timothy Atkins
  • Patent number: 9012208
    Abstract: A self-contained apparatus for isolating nucleic acid, cell lysates and cell suspensions from unprocessed samples apparatus, to be used with an instrument, includes at least one input, and: (i) a macrofluidic component, including a chamber for receiving an unprocessed sample from a collection device and at least one filled liquid purification reagent storage reservoir; and (ii) a microfluidic component in communication with the macrofluidic component through at least one microfluidic element, the microfluidic component further comprising at least one nucleic acid purification matrix; and (iii) at least one interface port to a drive mechanism on the instrument for driving said liquid purification reagent, through the microfluidic element and the nucleic acid purification matrix, wherein the only inputs to the apparatus are through the chamber and the interface port to the drive mechanism.
    Type: Grant
    Filed: February 3, 2010
    Date of Patent: April 21, 2015
    Assignee: NetBio, Inc.
    Inventors: Richard F. Selden, Eugene Tan
  • Patent number: 8993314
    Abstract: A system for culturing photosynthesizing microorganisms includes a source of a gaseous fluid a mixer that creates micron bubbles within an aqueous medium using the gaseous fluid. The mixing chamber holds the aqueous medium including the micron bubbles before the micron bubbles and aqueous medium are mixed with a culture of photosynthesizing microorganism in a reaction chamber.
    Type: Grant
    Filed: July 28, 2007
    Date of Patent: March 31, 2015
    Assignee: Ennesys SAS
    Inventors: Nicholas Eckelberry, T. Riggs Eckelberry
  • Patent number: 8986976
    Abstract: The present disclosure provides a method to isolate natural & artificial nucleic acids like deoxyribonucleic acid (DNA), ribonucleic acid (RNA) and peptide nucleic acid (PNA) from a solid or liquid sample using cotton. The cotton packed is such that, a solution containing nucleic acids passes through it and the nucleic acids in solution are bound to the cotton in a medium optimal for binding. The nucleic acids are bound to cotton in such a way that, the bound nucleic acids can withstand multiple washes with liquid comprising water and gets eluted in an aqueous buffer, with which eluted nucleic acids can be directly used for amplification using PCR or for any other biochemical or molecular biology needs.
    Type: Grant
    Filed: January 6, 2011
    Date of Patent: March 24, 2015
    Assignee: Bigtec Private Limited
    Inventors: Phani Kumar Pullela, Mulakkapurath Narayanan Manoj, Santhosh Kumar Gandhavalla, Mitchell Preetham Pinto, Chandrasekhar Bhaskaran Nair, Pillarisetti Venkata Subbarao
  • Publication number: 20140342439
    Abstract: Apparatuses, kits, and methods for portable sample disruption (e.g., for encouraging cell lysis).
    Type: Application
    Filed: May 28, 2014
    Publication date: November 20, 2014
    Applicant: ZYMO RESEARCH CORPORATION
    Inventors: Nikolas M. ISELY, Marc E. VAN EDEN, Jonathan K. BROWN
  • Patent number: 8865452
    Abstract: Presented herein are exemplary systems and methods for extracting lipids from a wet algal biomass. An exemplary method comprises lysing a wet algal biomass with an insoluble granular lysing agent to create a lysate, creating a lipid-rich phase in the lysate, and separating the lipid-rich phase from the lysate. An exemplary system comprises a lysing station for creating a lysate from a wet algal biomass and a separation station for creating and separating a lipid-rich phase from the lysate. According to further exemplary systems and methods, ultrasound may be used in place of or in addition to a lysing agent to lyse the wet algal biomass.
    Type: Grant
    Filed: June 15, 2009
    Date of Patent: October 21, 2014
    Assignee: Aurora Algae, Inc.
    Inventors: Guido Radaelli, Daniel Fleischer
  • Patent number: 8835152
    Abstract: Compositions of peptides and surface-active agents are described, as are methods of making and using such compositions. The compositions are capable of affecting metabolic rates in biological systems, and to accelerate nutrient uptake without a concomitant increase in biofilm production.
    Type: Grant
    Filed: December 4, 2012
    Date of Patent: September 16, 2014
    Assignee: Advanced Biocatalytics Corporation
    Inventor: Carl W. Podella
  • Publication number: 20140255299
    Abstract: In an aspect, the invention relates to compositions and methods for permeabilizing membranes of cells. In an aspect, the invention relates to compositions and methods for killing cells. In an aspect, the invention relates to compositions and methods of permeabilizing the membranes of cancer cells or microbial cells.
    Type: Application
    Filed: August 24, 2012
    Publication date: September 11, 2014
    Applicant: UNIVERSITY OF CENTRAL FLORIDA RESEARCH FOUNDATION, INC.
    Inventors: Annette Khaled, Rebecca Boohaker, Michael Lee, Jesus Perez Figueroa
  • Publication number: 20140242678
    Abstract: The present invention provides an apparatus and a method for a lysis procedure, in particular for an automated and/or controlled lysis procedure of a sample, in particular a biological sample.
    Type: Application
    Filed: July 27, 2011
    Publication date: August 28, 2014
    Inventors: Andreas Boos, Gerd Lüdke, Johannes Bacher
  • Patent number: 8815521
    Abstract: An apparatus for disrupting cells or viruses comprises a container having a chamber for holding the cells or viruses. The container includes at least one flexible wall defining the chamber. The apparatus also includes a transducer for impacting an external surface of the flexible wall to generate pressure waves in the chamber. The apparatus also includes a pressure source for increasing the pressure in the chamber. The pressurization of the chamber ensures effective coupling between the transducer and the flexible wall. The apparatus may also include beads in the chamber for rupturing the cells or viruses.
    Type: Grant
    Filed: September 22, 2005
    Date of Patent: August 26, 2014
    Assignee: Cepheid
    Inventors: Michael T. Taylor, Farzad Pourahmadi, William A. McMillan, Ronald Chang
  • Publication number: 20140141497
    Abstract: A chemostat is described that includes a growth chamber having a plurality of compartments. Each of the compartments may be fluidly isolated from the rest of the growth chamber by one or more actuatable valves. The chemostat may also include a nutrient supply-line to supply growth medium to the growth chamber, and an output port to remove fluids from the growth chamber. Also, a method of preventing biofilm formation in a growth chamber of a chemostat is described. The method may include the steps of adding a lysis agent to a isolated portion of the growth chamber, and reuniting the isolated portion with the rest of the growth chamber.
    Type: Application
    Filed: March 26, 2013
    Publication date: May 22, 2014
    Applicant: California Institute of Technology
    Inventors: Frederick Balagadde, Carl L. Hansen, Emil Kartalov, Stephen R. Quake
  • Publication number: 20140087359
    Abstract: Disclosed herein are methods and systems for use in preparing a sample. The methods and systems may be used for lysing one or more structures in a sample (e.g., cells, viral particles, etc.). The methods and compositions may comprise a microfluidic chip or use thereof. The microfluidic chips disclosed herein may comprise (a) a substrate comprising a chamber, wherein at least one mechanical element may be located within the chamber; (b) a thermal element in contact with the chamber; and (c) at least one aperture within the surface of the substrate, wherein the aperture may be configured to insulate the chamber.
    Type: Application
    Filed: September 20, 2013
    Publication date: March 27, 2014
    Applicant: California Institute of Technology
    Inventors: Samuel NJOROGE, John GORMAN, George MALTEZOS, Axel SCHERER
  • Patent number: 8679797
    Abstract: The present invention provides a method for extracting glucan and mannan from the cell wall of a microorganism. Specifically, the method of the present invention in one embodiment comprises the steps of: a) treating the cells of the microorganism with an alkaline protease and an mannanase; b) separating the mixture from step a) into a heavy phase and a light phase; c) drying the heavy phase obtained from step b), obtaining the glucan preparation; and d) drying the light phase obtained from step b), obtaining the mannan preparation. Optionally, in the step c), the heavy phase obtained from step b) may be treated sequentially with an alkali and an acid, and separated again into a heavy phase and a light phase. The heavy phase is dried, obtaining the glucan preparation. The present invention further relates to the glucan preparation and mannan preparation produced thereby, and the uses thereof.
    Type: Grant
    Filed: December 31, 2008
    Date of Patent: March 25, 2014
    Assignee: Angel Yeast Co., Ltd.
    Inventors: Xuefeng Yu, Zhihong Li, Minghua Yu, Juan Yao, Yan Zhang
  • Publication number: 20130330767
    Abstract: A process for cell lysis is provided. The process comprises passing a mixture comprising a suspension of cells and a lysis reagent through a flow-through reactor, wherein the mixture passes through the reactor with pulsed or superimposed oscillating flow. The process is preferably employed for the preparation of biological products, such as pDNA or inclusion bodies.
    Type: Application
    Filed: February 17, 2012
    Publication date: December 12, 2013
    Applicant: FUJIFILM DIOSYNTH BIOTECHNOLOGIES UK LIMITED
    Inventors: John MacDonald Liddell, Azam Razzaq
  • Patent number: 8592157
    Abstract: An analyte is separated from a fluid sample by introducing the sample into a cartridge having a sample port and a first flow path extending from the sample port. The first flow path includes an extraction chamber containing a solid support for capturing the analyte from the sample. The cartridge has a second flow path for eluting the captured analyte from the extraction chamber, the second flow diverging from the first flow path after passing through the extraction chamber. The sample is forced to flow through the extraction chamber and into a waste chamber, thereby capturing the analyte with the solid support as the sample flows through the extraction chamber. The captured analyte is then eluted from the extraction chamber by forcing an elution fluid to flow through the extraction chamber and along the second flow path.
    Type: Grant
    Filed: July 19, 2012
    Date of Patent: November 26, 2013
    Assignee: Cepheid
    Inventors: Kurt E. Petersen, William A. McMillan, Lee A. Christel, Ronald Chang, Farzad Pourahmadi, Jesus Ching, Gregory T. A. Kovacs, M. Allen Northrup
  • Patent number: 8546127
    Abstract: Apparatus and method for collection of a target material from a liquid sample comprising target species that contain the target material. The apparatus comprises a fluid flow conduit in communication with a filter medium having a pore size adapted to retain target species thereon and pass, as filtrate, lysate containing the desired target material. A lysing agent conduit communicates with the fluid flow conduit and delivers lysing agent to the filter medium to lyse the target cells thereby releasing the desired intracellular target material. The lysing agent may be recirculated through the filter medium for a sufficient time to permit sufficient quantity of lysate to circulate through the system for lysate collection and subsequent assay.
    Type: Grant
    Filed: June 30, 2008
    Date of Patent: October 1, 2013
    Assignee: General Electric Company
    Inventor: Dean Marschke
  • Patent number: 8524449
    Abstract: The present invention relates a method for the enumeration of mammalian cell micronuclei, while distinguishing micronuclei from the chromatin of dead and dying cells. The method utilizes differential staining of chromatin from dead and dying cells, to distinguish the chromatin from micronuclei and nuclei that can be detected based upon fluorescent emission and light scatter following exposure to an excitatory light source. Counting of micronuclei events relative to the number of nuclei can be used to assess the DNA-damaging potential of a chemical agent, the DNA-damaging potential of a physical agent, the effects of an agent which can modify endogenously-induced DNA damage, and the effects of an agent which can modify exogenously-induced DNA damage. Kits for practicing the invention are also disclosed.
    Type: Grant
    Filed: January 8, 2010
    Date of Patent: September 3, 2013
    Assignee: Litron Laboratories, Ltd.
    Inventors: Stephen D. Dertinger, Sian E. Cairns, Svetlana L. Avlasevich, Dorothea K. Torous
  • Patent number: 8512998
    Abstract: Conditioning and concentration of microalgae are accomplished by the process steps of grinding a dilute aqueous dispersion of microalgae in the presence of grinding media and then applying adsorptive bubble separation. This process is amenable to the use of dilute feed microalgal dispersions such as are encountered in the production of algal biomass for biofuel applications.
    Type: Grant
    Filed: June 18, 2008
    Date of Patent: August 20, 2013
    Assignees: Renewable Algal Energy, LLC, Eastman Chemical Company
    Inventors: Robert L. Clayton, Stephen N. Falling, Jeffrey S. Kanel
  • Patent number: 8481261
    Abstract: The present invention relates to a nucleic acid extracting apparatus, and the nucleic acid extracting apparatus can include a pipe-shaped tube having an open outlet at one side thereof, and a hydrogel column that is provided inside the tube and filters impurities excluding an extraction target material.
    Type: Grant
    Filed: June 22, 2009
    Date of Patent: July 9, 2013
    Assignees: Postech Academy-Industry Foundation, Catholic University Industry-Academic Cooperation Foundation
    Inventors: Geun-Bae Lim, Ji-Min Kahng, Tae-Hee Kang, Jin-Hwa Jung
  • Patent number: 8476060
    Abstract: A novel low cost separation process for separating lipid oil from an algal biomass for biofuel production is described herein. The process of the present invention comprises of two steps: (i) breaking the algae cells and (ii) separation of the lipid oils from the broken cells. The separated lipids are extracted by conventional techniques followed by conversion to a biofuel.
    Type: Grant
    Filed: April 12, 2010
    Date of Patent: July 2, 2013
    Assignee: Board of Regents, The University of Texas System
    Inventors: Russell Chianelli, Calvin Hildebrand, Joaquin Rodriguez
  • Patent number: 8435465
    Abstract: A microfluidic cartridge for isolating biological molecules having a capture chamber containing functionalized solid supports maintained in a fluidized state provides reduced pressure drops and bubble formation during microfluidic extraction. The cartridge may include an electric field lysis chamber and/or a chemical lysis chamber. The electric-field lysis chamber may comprise an electrically insulating structure arranged between two opposing planar electrodes.
    Type: Grant
    Filed: November 3, 2008
    Date of Patent: May 7, 2013
    Assignee: CFD Research Corporation
    Inventors: Shivshankar Sundaram, Balabhaskar Prabhakarpandian, Kapil Pant, Yi Wang
  • Publication number: 20130065223
    Abstract: The present invention provides a universally applicable lysis buffer comprising a chaotropic 5 agent, a reducing agent, and a proteolytic enzyme suitable for processing a wide variety o different sample types, such as different types of bodily samples relevant for the diagnosis o a respiratory disease. Furthermore, the present invention provides the use of a chaotropic agent, a reducing agent, and a proteolytic enzyme for the lysis of a broad spectrum of bodily samples. Moreover, the present invention provides a method for processing bodily samples which is universally applicable to the lysis of a variety of different types of bodily samples. Furthermore, the present invention provides methods for analyzing a bodily sample or for detecting the presence of a pathogen in a bodily sample, preferably, for diagnosing a respiratory disease, such as pneumonia or tuberculosis.
    Type: Application
    Filed: May 9, 2011
    Publication date: March 14, 2013
    Applicant: CURETIS AG
    Inventors: Matthias Klein, Gerd Lüdke, Andreas Boos
  • Patent number: 8372340
    Abstract: Cartridges for the isolation of a biological sample and downstream biological assays on the sample are provided. In one embodiment, a nucleic acid sample is isolated from a biological sample and the nucleic acid sample is amplified, for example by the polymerase chain reaction. The cartridges provided herein can also be used for the isolation of non-nucleic acid samples, for example proteins, and to perform downstream reactions on the proteins, for example, binding assays. Instruments for carrying out the downstream biological assays and for detecting the results of the assays are also provided.
    Type: Grant
    Filed: February 23, 2011
    Date of Patent: February 12, 2013
    Assignee: Luminex Corporation
    Inventors: Dylan Hilmer Bird, Jesus Ching, Bruce A. Johnson, Keith E. Moravick, Bruce Richardson
  • Patent number: 8268603
    Abstract: An apparatus for disrupting cells or viruses comprises a container having a chamber for holding the cells or viruses. The container includes at least one flexible wall defining the chamber. The apparatus also includes a transducer for impacting an external surface of the flexible wall to generate pressure waves in the chamber. The apparatus also includes a pressure source for increasing the pressure in the chamber. The pressurization of the chamber ensures effective coupling between the transducer and the flexible wall. The apparatus may also include beads in the chamber for rupturing the cells or viruses.
    Type: Grant
    Filed: September 22, 2005
    Date of Patent: September 18, 2012
    Assignee: Cepheid
    Inventors: Michael T. Taylor, Phillip Belgrader, Farzad Pourahmadi, William A. McMillan, Ronald Chang, Jesus Ching
  • Patent number: 8247176
    Abstract: An analyte is separated from a fluid sample by introducing the sample into a cartridge having a sample port and a first flow path extending from the sample port. The first flow path includes an extraction chamber containing a solid support for capturing the analyte from the sample. The cartridge has a second flow path for eluting the captured analyte from the extraction chamber, the second flow diverging from the first flow path after passing through the extraction chamber. The sample is forced to flow through the extraction chamber and into a waste chamber, thereby capturing the analyte with the solid support as the sample flows through the extraction chamber. The captured analyte is then eluted from the extraction chamber by forcing an elution fluid to flow through the extraction chamber and along the second flow path.
    Type: Grant
    Filed: March 7, 2011
    Date of Patent: August 21, 2012
    Assignee: Cepheid
    Inventors: Kurt E. Petersen, William A. McMillan, Lee A. Christel, Ronald Chang, Farzad Pourahmadi, Jesus Ching, Gregory T. A. Kovacs, M. Allen Northrup
  • Patent number: 8202693
    Abstract: A method of isolation of nucleic acids from a biological sample of cells comprising a combination of a solid phase cell nuclei isolation procedure with a solid phase nucleic acid isolation method.
    Type: Grant
    Filed: November 24, 2009
    Date of Patent: June 19, 2012
    Assignee: Omega Bio-Tek, Inc.
    Inventor: Qi Guo
  • Patent number: 8192926
    Abstract: The present invention is directed to methods for extracting markers from biological samples, and to systems, devices, kits and reagents for use in such methods. The invention is also to methods, kits, reagents and compositions for measuring a plurality of different organism types in a sample. One of the specific advantages of the present invention is the ability to simultaneously extract more than one microorganism or viral particle marker in one volume from a single sample containing a complex biological matrix.
    Type: Grant
    Filed: May 19, 2006
    Date of Patent: June 5, 2012
    Assignee: Meso Scale Technologies LLC
    Inventors: Jeff D. Debad, Cindy V. Ly
  • Patent number: 8187837
    Abstract: A process for the production of a purified recombinant GDF-5 related protein in prokaryotes comprises the steps of bacterial cell disruption and inclusion body solubilization to obtain a solubilized monomer of a GDF-5 related protein, said process characterized by a) disruption of bacterial cells with a high pressure homogenizer at a disruption pressure between 800 and 900 bar; and/or b) treatment of the recovered inclusion bodies with a denaturing solubilization buffer comprising L-arginine.
    Type: Grant
    Filed: June 20, 2008
    Date of Patent: May 29, 2012
    Assignee: Biopharm Gesellschaft zur biotechnologischen Entwicklung von Pharmaka mbH
    Inventors: Ute Ehringer, Eva Kohlstrung
  • Patent number: 8187861
    Abstract: For extracting phosphorous from algae-in a body of water, the algae are deposited into a sealable tank (200) that is then evacuated, thereby rupturing the algal cell walls. The ruptured algae are then moved to a second tank (260), mixed with water and bacterial cultures, and allowed to settle until the lipids rise to the top and the oil-less debris settles to the bottom. The second tank also contains sacrificial (295) and rusted electrodes (320). The phosphorous from the algae combines with the rust. The lipids and debris are then removed. Next, an electrical source (315) causes the rust to be removed from the rusted electrodes and settle. In addition, a phosphorous-rich scum floats to the top. These components are placed in storage containers for later use and the water is returned, thereby reducing its phosphorous content.
    Type: Grant
    Filed: February 26, 2010
    Date of Patent: May 29, 2012
    Inventor: Allen John Schuh
  • Patent number: 8168442
    Abstract: A cartridge for conducting a chemical reaction includes a body having at least one flow path formed therein. The cartridge also includes a reaction vessel extending from the body for holding a reaction mixture for chemical reaction and optical detection. The vessel comprises a rigid frame defining the side walls of a reaction chamber. The frame includes at least one channel connecting the flow path to the chamber. The vessel also includes flexible films or sheets attached to opposite sides of the rigid frame to form opposing major walls of the chamber. In addition, at least two of the side walls are optically transmissive and angularly offset from each to permit real-time optical detection of analyte in the reaction chamber.
    Type: Grant
    Filed: September 29, 2004
    Date of Patent: May 1, 2012
    Assignee: Cepheid
    Inventors: Kurt E. Petersen, William A. McMillan, Farzad Pourahmadi, Ronald Chang, Douglas B. Dority
  • Patent number: 8158405
    Abstract: A method of treating a liquid sample having microbiological target species therein to concentrate the species and collect lysate is disclosed. The liquid sample comprises non-target microbiological particles, inorganic particles, and microbiological target species. The liquid is passed through a prefilter medium to allow the target species to pass through as filtrate and retain non-target microbiological products and inorganic particles thereon. The filtrate is contacted with a main filtration medium adapted to retain the target species thereon as retentate. The retentate is lysed to form a lysate containing target material that was enveloped within the microbiological target species. The microbiological species may comprise cell containing or viral material. Target materials comprise intracellular nucleic acids, or in the case of viral sampling, nucleic acids encased within the protein sheath or coating of the virus.
    Type: Grant
    Filed: June 30, 2008
    Date of Patent: April 17, 2012
    Assignee: General Electric Company
    Inventors: Scott Boyette, Rong Xu, Jing Luo, Weiqing Xu, Jing Chen, Weimin Xiao, Tong Chen
  • Publication number: 20110294128
    Abstract: The present invention relates to a method for isolating microorganisms and/or microorganisms nucleic acids from a bodily fluid that may comprise or may be suspected to comprise microorganisms and/or host cells and/or host cells debris. Microorganisms nucleic acids may further be isolated by lysing the isolated microorganisms. The present invention also relates to a method for detecting microorganisms in a bodily fluid. The present invention further relates to a saponin formulation and its use.
    Type: Application
    Filed: August 1, 2008
    Publication date: December 1, 2011
    Applicant: Universite Laval
    Inventors: Régis Peytavi, Ann Huletsky, Lucile Belley-Montfort, Isabelle Martineau
  • Publication number: 20110281272
    Abstract: The present invention provides a lysis buffer comprising a chaotropic agent and a reducing agent suitable for liquefaction of a highly viscous liquid biological sample, such as sputum, a use of said lysis buffer for the processing of a highly viscous liquid biological sample, methods for processing or analyzing a highly viscous liquid biological sample, or a method for detecting a pathogen within a highly viscous liquid biological sample. Furthermore, the present invention relates to a lysate comprising a highly viscous liquid biological sample and the lysis buffer according to the present invention, a ready-to-use reaction tube and a kit comprising the lysis buffer according to the present invention.
    Type: Application
    Filed: January 18, 2010
    Publication date: November 17, 2011
    Inventor: Matthias Klein
  • Publication number: 20110275132
    Abstract: Presented are methods of isolation of pili and pilus-like structures from Gram-positive bacteria including Streptococcus pneumoniae and compositions that include such isolated pili. These compositions are useful as immunogenic compositions for the production of antibodies and immunostimulation. Also presented are methods of inhibiting Streptococcus pneumoniae, and methods of identifying inhibitors of Streptococcus pneumoniae.
    Type: Application
    Filed: May 26, 2011
    Publication date: November 10, 2011
    Applicant: NOVARTIS AG
    Inventors: Antonello COVACCI, Markus HILLERINGMANN, Ilaria FERLENGHI
  • Patent number: 8043496
    Abstract: A method and apparatus for extracting oil from algae comprises a disruptor (125) having a plurality of deflectors (220 and 225) against which a mixture of algae and water (100) is forcibly impacted upon the urging of a pump (115). The impacting of the algal cells against the deflectors ruptures their cell walls and liberates the lipids (oil) and other materials contained therein. A tank (130) collects the mixture and after a settling period, the mixture forms at least three layers comprising oil (155), water (160), and algal residue (165). The oil layer is removed through one or more conduits (177, 178) into a holding tank (185) for further refining and use. The water is discarded, and the biomass residue comprising algal cell walls and other non-oil components is removed to another holding container (199) from which it can be discarded or used as an agricultural fertilizer or the like. If desired, the residue can be further treated in order to scavenge any remaining oil.
    Type: Grant
    Filed: December 22, 2008
    Date of Patent: October 25, 2011
    Inventors: Peter Allen Schuh, Allen John Schuh
  • Publication number: 20110250680
    Abstract: The present invention relates to, among other things, a device for collecting airborne microorganisms, said device having: an air collecting module, comprising: i. an upper element having an air admission duct permitting entry of an air stream into said module, said duct being provided, at its base, with means for disturbance of the air stream, ii. a lower element having air evacuating means permitting the air stream created to exit and said upper and lower elements can be made integral with one another so that the air stream can be created within said air collecting module; a cartridge, of roughly cylindrical shape, having a microorganism retention zone, said retention zone having means for lysis of the microorganisms, said cartridge being positioned within said air collecting module.
    Type: Application
    Filed: December 9, 2009
    Publication date: October 13, 2011
    Applicant: BIOMERIEUX
    Inventors: Patrick Broyer, Agnes Dupontfilliard, Massimo Galdiero, Michel Guy, Hermanus Johannes Maria Kreuwel, Emiliano Maione, Emiel Gerebern Maria Verwimp
  • Patent number: 8003369
    Abstract: The present invention is a bacteriolytic agent containing a cationic surfactant (A) of which a counteranion is an acid having a pKa (25° C.) of 0 to 10. As the counterion, a carboxylate anion is preferable. As the cationic surfactant (A), a quaternary ammonium salt-type surfactant is preferable. Examples of the useful substance include a protein, an amino acid, a nucleic acid, an antibiotic, sugars or vitamins. The bacteriolytic agent of the present invention is excellent in a bacteriolytic power in a step of extracting a useful substance from a microorganism (useful substance producing bacterium etc.). In addition, denaturation of the useful substance during the step is small.
    Type: Grant
    Filed: November 6, 2006
    Date of Patent: August 23, 2011
    Assignee: Sanyo Chemical Industries, Ltd.
    Inventors: Shunichiro Yamaguchi, Shuji Miura, Hiroaki Maeda
  • Publication number: 20110195495
    Abstract: The inventive self-contained apparatus for isolating nucleic acid, cell lysates and cell suspensions from unprocessed samples apparatus, to be used with an instrument, comprises at least one input, and: (i) a macrofluidic component, comprising a chamber for receiving said unprocessed sample from a collection device and at least one filled liquid purification reagent storage reservoir; and (ii) a microfluidic component in communication with said macrofluidic component via at least one microfluidic element, said microfluidic component further comprising at least one nucleic acid purification matrix; and (iii) a drive mechanism on said instrument for driving said liquid purification reagent, through said microfluidic element and said nucleic acid purification matrix, wherein the only inputs to said apparatus are via said chamber and said drive mechanism.
    Type: Application
    Filed: February 11, 2011
    Publication date: August 11, 2011
    Applicant: NETBIO, INC.
    Inventors: Richard F. Selden, Eugene Tan
  • Publication number: 20110189759
    Abstract: The invention relates to a method and a device for the digestion of biological cells. Digestion of cells is understood as breaking up cells so that hereditary information can be removed. Using the present invention, nucleic acids are to be able to be removed from cells in particular. The nucleic acids include RNA and DNA. According to the invention, cells are digested in a vessel which can be connected to a line and can thus be part of an overall device. Liquid can drain out of the container or can be pumped into the container via the line, for example. The typical means for performing a mechanical digestion are located in the vessel, that is, in particular beads and a buffer solution. In contrast to the prior art, however, the vessel is not shaken, that is, subjected to vibrations. Instead, it is stirred with the aid of a magnetic stirrer. Strong vibrations are thus avoided. A vessel for performing digestion of cells can therefore be integrated without problems in an overall device.
    Type: Application
    Filed: January 15, 2009
    Publication date: August 4, 2011
    Inventors: Ralf Himmelrich, Thomas Rothmann
  • Publication number: 20110177521
    Abstract: The invention generally relates to methods and kits for isolating nucleic acids from an organism. In certain embodiments, methods of the invention involve contacting a plurality of lytic enzymes to an organism, thereby lysing a cell wall of the organism to release the nucleic acid, and introducing at least one agent to separate the nucleic acid from the lysed cells, thereby isolating the nucleic acid.
    Type: Application
    Filed: January 19, 2010
    Publication date: July 21, 2011
    Applicant: OpGen, Inc.
    Inventor: John Kevin Henkhaus
  • Publication number: 20110092687
    Abstract: The invention relates to a lysis buffer mixture that is stable in storage for isolating nucleic acids from biological, preferably diagnostic samples. The mixture is preferably associated with an extraction control. The aim of the invention is to provide an improved nucleic acid extraction system, which is cost-effective, stable and easy to use, thus fulfilling the requirements of a modern nucleic acid extraction system and containing, among other things, extraction controls. The invention relates to a lysis buffer mixture for isolating nucleic acids, said mixture containing non chaotropic salts, a special selection of detergents, a defined quantity of at least one nucleic acid as an extraction control, optionally lytic enzymes, optionally carrier nucleic acids and optionally other additives.
    Type: Application
    Filed: April 20, 2009
    Publication date: April 21, 2011
    Inventors: Peter Bendzko, Hans Joos
  • Patent number: 7919436
    Abstract: The invention provides genes that are unique either to Ehrlichia ruminantium strain Gardel or to Ehrlichia ruminantium strain Welgevonden, or allelic couples which are present in both strains but whose sequences differ between the two strains, as genetic markers to differentiate between these two strains. The invention also provides diagnostic methods using these genetic markers.
    Type: Grant
    Filed: October 22, 2004
    Date of Patent: April 5, 2011
    Assignees: Centre de Cooperation Internationale en Recherche Agronomique pour le Developpement (CIRAD), Centre National de la Recherche Scientifique
    Inventors: Roger Frutos, Conception Ferraz, Jacques Demaille, Dominique Martinez