Micro-organism, Per Se (e.g., Protozoa, Etc.); Compositions Thereof; Proces Of Propagating, Maintaining Or Preserving Micro-organisms Or Compositions Thereof; Process Of Preparing Or Isolating A Composition Containing A Micro-organism; Culture Media Therefor Patents (Class 435/243)
  • Patent number: 11162120
    Abstract: Described is a method for the enzymatic production of an acyl phosphate from a 2-hydroxyaldehyde using a phosphoketolase or a sulfoacetaldehyde acetyltransferase.
    Type: Grant
    Filed: September 8, 2016
    Date of Patent: November 2, 2021
    Assignees: Scientist of Fortune, S.A., Global Bioenergies
    Inventor: Philippe Marlière
  • Patent number: 11154858
    Abstract: Disclosed herein are various bioreactor devices and systems for growing cellular material, and related methods of growing cellular material. In some cases, a system can include a bioreactor system having one or more wells with at least one fluidic passageway coupled to each well to feed fluids to biological material being developed inside the well. In some cases, a bioreactor system can include a main body comprising the perimeter of the well and the fluidic passageways, a cover that forms the top of the well and provides optical access into the well, and a base that forms the bottom surface of the well. The cover and base can be attached and detached from the main body to seal the well closed and to physically access the contents of the well.
    Type: Grant
    Filed: October 6, 2016
    Date of Patent: October 26, 2021
    Assignee: University of Pittsburgh—Of the Commonwealth System of Higher Education
    Inventors: Riccardo Gottardi, Peter Alexander, Bryan Romell, Rocky S. Tuan
  • Patent number: 11118197
    Abstract: In one aspect, the present invention provides methods for preparing a fermentation product. The methods include pre-treating a mixture of biomass and ionic liquid, wherein the ionic liquid comprises a choline cation and the biomass comprises polysaccharide and lignin. The methods further include forming hydrolysates from the introduction of glycoside hydrolase to the pre-treated mixture at conditions sufficient to produce a sugar composition mixture for fermentation steps. The present invention provides methods for loading biomass mixtures in a batch-fed process, wherein the biomass slurries can be loaded into water or a concentrated sugar composition for hydrolysate production. The methods can be performed in a one-pot process, wherein the ionic liquids are present in the mixtures throughout each step. Aspects of the invention provide compositions of sugar composition mixtures and fermentation product mixtures.
    Type: Grant
    Filed: November 21, 2016
    Date of Patent: September 14, 2021
    Assignee: National Technology & Engineering Solutions of Sandia, LLC
    Inventors: Feng Xu, Blake Simmons, Seema Singh
  • Patent number: 11053302
    Abstract: The present invention relates to single domain antibodies comprising at least one modification relative to the 4D5 antibody scaffold or human germline VH3 domain, the modifications selected from the group consisting of H35D, A78V, S93V, S93G and W103R, with the position numbering being according to the Kabat numbering scheme. Disulfide-free variants further comprise at least one additional modification selected from the group consisting of C22S, A24I, A24L and C92T, and with the proviso that at least one of C22S and C92T is present. Further encompassed are the multi-modular antibody molecules and antibody conjugates comprising single domain antibodies, as well as methods for producing them. The invention in particular provides a library of the single domain antibodies or multi-modular antibody molecules and a method for selecting an antibody that binds an antigen.
    Type: Grant
    Filed: November 4, 2015
    Date of Patent: July 6, 2021
    Assignee: Nanyang Technological University
    Inventors: Tobias Carl Cornvik, Ignacio Jose Asial, Par Lennart Nordlund
  • Patent number: 10996149
    Abstract: A device and a method for isolating a target from a biological sample are provided. The target is bound to solid phase substrate to form target bound solid phase substrate. The device includes a lower plate with an upper surface having a plurality of regions. The biological sample is receivable on a first of the regions. An upper plate has a lower surface directed to the upper surface of the lower plate. A force is positioned adjacent the upper plate and attracts the target bound solid phase substrate toward the lower surface of the upper plate. At least one of the upper plate and the lower plate is movable from a first position wherein the target bound solid phase substrate in the biological sample are drawn to the lower surface of the upper plate and a second position wherein the target bound solid phase substrate are isolated from the biological sample.
    Type: Grant
    Filed: August 30, 2017
    Date of Patent: May 4, 2021
    Assignee: Wisconsin Alumni Research Foundation
    Inventors: David J. Beebe, Ben P. Casavant, David J. Guckenberger, Scott M. Berry
  • Patent number: 10842870
    Abstract: The present invention relates to humanized anti-human GPVI antibodies and uses thereof.
    Type: Grant
    Filed: August 5, 2016
    Date of Patent: November 24, 2020
    Assignees: ACTICOR BIOTECH, UNIVERSITE DE PARIS, UNIVERSITÉ PARIS-XIII, INSTITUT NATIONAL DE LA SANTÉ ET DE LA RECHERCHE MÉDICALE (INSERM), UNIVERSITE PARIS-SACLAY
    Inventors: Philippe Billiald, Martine Jandrot-Perrus
  • Patent number: 10781417
    Abstract: An apparatus for processing cells is disclosed. In one embodiment, a fixed bed reactor is provided for the cells, the fixed bed reactor including a portion movable from a first position corresponding to a packed condition of the fixed bed to a second position corresponding to a depacked condition of the fixed bed. Movement of the partition facilitates harvesting of the cells there from. Related apparatus, kits, methods, and systems are also disclosed.
    Type: Grant
    Filed: December 11, 2013
    Date of Patent: September 22, 2020
    Assignees: PALL TECHNOLOGY UK LIMITED, PALL ARTELIS BVBA, PALL LIFE SCIENCES BELGIUM B.V.B.A.
    Inventors: Suresh Purushothaman, Jose Antonio Castillo Gonzalez, Jean-Christophe Drugmand, Vishwas Pethe, Derek Pendlebury, Fabien Moncaubeig
  • Patent number: 10745493
    Abstract: A graft copolymer comprising: a core polymer comprising a crosslinked or non-crosslinked polysaccharide, a plurality of primary graft polymers covalently grafted to the core polymer, a plurality of secondary graft polymers covalently grafted to each primary graft polymer, an injectable dermal aesthetic formulation comprising such a graft copolymer and a method of preparing such a graft copolymer.
    Type: Grant
    Filed: December 29, 2015
    Date of Patent: August 18, 2020
    Assignee: GALDERMA S.A.
    Inventor: Kristoffer Bergman
  • Patent number: 10704065
    Abstract: There is described a method for producing polymeric 3-hydroxypropionamide (3HP amide), the method comprising: culturing an Acetobacter lovaniensis-bacterium in a growth medium containing phosphate and ammonium, wherein culturing of the bacterium produces polymeric 3HP amide. The polymeric 3HP amide may then be hydrolysed to 3HP amide or converted to other compounds of interest.
    Type: Grant
    Filed: January 30, 2017
    Date of Patent: July 7, 2020
    Assignee: VERDANT BIOPRODUCTS LIMITED
    Inventor: Irene Finnegan
  • Patent number: 10626423
    Abstract: A non-naturally occurring microorganism having a 1,3-BDO pathway is provided. The microorganism expresses at least one of the following 1,3-BDO pathway enzymes: an aldolase that catalyzes condensation of two acetaldehydes to produce 3-hydroxybutanal; and an aldo-ketoreductase, oxidoreductase, aldehyde reductase or alcohol dehydrogenase that reduces 3-hydroxybutanal to 1,3-BDO. The organism may further express one or more enzymes for producing acetaldehyde. A biosynthetic process involves condensing two acetaldehyde molecules to 3-hydroxybutanal using an enzyme from class aldolases; and selectively reducing 3-hydroxybutanal to 1,3-BDO using an enzyme belonging to the class aldo-ketoreductase, oxidoreductase, aldehyde reductase or alcohol dehydrogenase. The process can further include producing acetaldehyde by a biosynthetic method.
    Type: Grant
    Filed: July 21, 2016
    Date of Patent: April 21, 2020
    Assignee: THE GOVERNING COUNCIL OF THE UNIVERSITY OF TORONTO
    Inventors: Radhakrishnan Mahadevan, Alexander Yakunin, Pratish Gawand, Kayla Nemr
  • Patent number: 10588280
    Abstract: Provided herein are delivery methods of a plant treatment agent to a tissue of a plant, wherein the tissue is contacted with a plant treatment agent and delivery is aided by a centrifugal force applied to the plant. Disclosed herein is the discovery that drastic improvements in treatment delivery occur when plants are centrifuged after being contacted, submerged, unsubmerged, soaked, etc. in a solution containing a plant treatment agent. In one embodiment, haploid plants contacted with a colchicine solution are centrifuged to reveal improved chromosomal doubling rates as compared to control plants.
    Type: Grant
    Filed: May 1, 2015
    Date of Patent: March 17, 2020
    Assignee: Monsanto Technology LLC
    Inventors: Huachun Larue, Fenggao Dong
  • Patent number: 10519409
    Abstract: A fluid transfer assembly for single use bioreactors includes a fluid transfer housing that can be mounted to the impeller shaft using a bearing that places the fluid transfer assembly directly below the lowest impeller but allows the impeller shaft to spin inside independently of the fluid transfer assembly. A fluid conduit connects the fluid transfer housing to a port in the single use bag wall which allows fluids to be introduced into the sparger and which also helps prevent the fluid transfer assembly from rotating with the impeller shaft.
    Type: Grant
    Filed: December 22, 2015
    Date of Patent: December 31, 2019
    Assignee: GE Healthcare Bio-Sciences Corp.
    Inventors: Richard Lee Damren, Colin R. Tuohey
  • Patent number: 10513722
    Abstract: Compositions, methods and kits are disclosed for synthesizing and amplifying pools of probes using precursor oligonucleotides. In some aspects the precursor is amplified and nicking enzymes are used to separate the full length probes from the amplification products. The methods enable the preparation of single stranded DNA probes of defined sequence and length that are suitable for use in target detection assays.
    Type: Grant
    Filed: May 6, 2016
    Date of Patent: December 24, 2019
    Inventors: Yuker Wang, Keith W. Jones, Ronald J. Sapolsky
  • Patent number: 10472605
    Abstract: A serum-free medium for the growth of mesenchymal stem cells comprises FGF, TGF-? and lipoprotein.
    Type: Grant
    Filed: February 16, 2015
    Date of Patent: November 12, 2019
    Assignee: National University of Ireland, Galway
    Inventors: Francis Peter Barry, Emma Jane Mooney, Josephine Mary Murphy, Georgina Margaret Shaw, Sean Patrick Gaynard
  • Patent number: 10457963
    Abstract: Nucleic acids and cells comprising a methyltransferase gene and/or a reductase gene are disclosed. These nucleic acids and cells may be used to produce branched (methyl)lipids, such as 10-methylstearate.
    Type: Grant
    Filed: September 20, 2017
    Date of Patent: October 29, 2019
    Assignee: Novogy, Inc.
    Inventors: Arthur J. Shaw, IV, Hannah Blitzblau, Donald V. Crabtree
  • Patent number: 10456448
    Abstract: The present technology provides methods and compositions for the treatment of inflammatory and/or tissue damage conditions. In particular, the use of Smad7 compositions delivered locally or systemically to a site of inflammation and/or tissue damage is described. Other specific embodiments concern treatment or prevention of side effects caused by radiation and/or chemotherapy, including but not limited to oral and gastric mucositis. Also provided are codon-optimized nucleic acids encoding for Smad7 fusion proteins.
    Type: Grant
    Filed: March 7, 2014
    Date of Patent: October 29, 2019
    Assignee: The Regents of the University of Colorado, a body corporate
    Inventors: Xiao-Jing Wang, Qinghong Zhang, Yosef Refaeli
  • Patent number: 10436795
    Abstract: Carbon-detected NMR is well-suited for mapping binding sites in intrinsically disordered regions of a polypeptides, and for mapping of binding motifs in intrinsically disordered regions with single-residue resolution. Provided are methods of carbon-detected NMR for determining the amino acids that mediate the interaction between an intrinsically disordered polypeptide or protein, or an intrinsically disordered region of a polypeptide, and a biomolecule such as another polypeptide or a nucleic acid.
    Type: Grant
    Filed: September 14, 2017
    Date of Patent: October 8, 2019
    Assignee: Institute For Cancer Research
    Inventors: Hong Cheng, Heinrich Roder
  • Patent number: 10412958
    Abstract: Provided is a device for vitrification-based cryopreservation which enables easy and reliable vitrification freezing of cells or tissues. The device of the present invention for cell or tissue cryopreservation by vitrification has a porous structure made of a material having a refractive index of 1.45 or less as a vitrification solution absorber.
    Type: Grant
    Filed: October 17, 2014
    Date of Patent: September 17, 2019
    Assignees: SCHOOL JURIDICAL PERSON KITASATO INSTITUTE, MITSUBISHI PAPER MILLS LIMITED
    Inventors: Kenji Momozawa, Atsushi Matsuzawa, Katsumitsu Susaki
  • Patent number: 10415074
    Abstract: A method for detecting lactic acid and/or acetic acid bacteria in a food-processing matrix is taught, using microbial flora including a lactic acid and/or acetic acid bacterial flora and a fungal flora. The bacterial flora contains an adenosine triphosphate of bacterial origin, and the fungal flora contains an adenosine triphosphate of fungal origin. The method comprises applying, to the matrix, before a first time limit, an antifungal having an antifungal action which is lethal, on the fungal flora, and at a second time limit, an antibiotic action which is non lethal, at a second time limit after the first time limit, on the bacterial flora. The microbial flora is detected between the first time limit and the second time limit; the lethal antifungal action releases, into the matrix, for the first time limit, adenosine triphosphate of fungal origin and in which the microbial flora is detected between the first time limit and the second time limit.
    Type: Grant
    Filed: January 25, 2016
    Date of Patent: September 17, 2019
    Inventor: Charles Cervin
  • Patent number: 10371695
    Abstract: The present invention relates to a method and tools for extracting information on a compounds influence on a cellular phenotype. The method of the invention may be used as a very efficient procedure for testing the efficacy or resistance of single drugs or combinations of drugs on cells from individual patients. Thus, the methods may be useful for predicting efficacy of a drug on a given patient. The methods are also useful for testing of compounds for toxicity, identifying drug targets for known or novel compounds.
    Type: Grant
    Filed: July 1, 2015
    Date of Patent: August 6, 2019
    Assignee: 2cureX ApS
    Inventors: Grith Hagel, Ole Thastrup
  • Patent number: 10364426
    Abstract: A process for reducing cell death in eukaryotic and prokaryotic organisms includes the steps of mixing the organisms with water in a first reactor, strobing light onto the mixture of organisms and water for a period of time, passing the strobed mixture into another reactor, and discharging the mixture from the another reactor. The step of strobing light includes strobing light to the mixture of organisms and water at a frequency of between 10 Hz and 40 Hz. The strobing of the light is between twenty flashes per second and eighty flashes per second. The first reactor is a continuously stirred reactor. The another reactor is a plug flow reactor. The organisms are in an algal culture.
    Type: Grant
    Filed: March 3, 2017
    Date of Patent: July 30, 2019
    Assignee: MISSING LINK TECHNOLOGY, LLC
    Inventor: Samuel L. Shepherd
  • Patent number: 10350554
    Abstract: A container assembly includes a flexible bag having an interior surface bounding a chamber and an opposing exterior surface, the bag having a bottom end wall being comprised of a first sheet overlying a second sheet, the first sheet and the second sheet of the bottom end wall being welded together so as to form a first sparging area and a separated second sparging area that are both bounded between the first sheet and the second sheet, at least a portion of the first sheet overlying the first and second sparging areas being gas permeable.
    Type: Grant
    Filed: March 29, 2017
    Date of Patent: July 16, 2019
    Assignee: Life Technologies Corporation
    Inventors: Michael E. Goodwin, Nephi D. Jones, Derik R. West
  • Patent number: 10342862
    Abstract: The invention relates to an immunization regimen whereby an infant is protected against respiratory syncytial virus (RSV) through administration of a first anti-RSV immune response inducing composition to his or her mother during pregnancy, followed by administration of a second anti-RSV immune response inducing composition to the infant after birth.
    Type: Grant
    Filed: January 26, 2012
    Date of Patent: July 9, 2019
    Assignee: GlaxoSmithKline Biologicals, SA
    Inventor: Phillip Dormitzer
  • Patent number: 10316281
    Abstract: A cell culture apparatus includes cell culture units having cell culture chambers, each with at least one manifold. The manifold connects the cell culture unit to a fluid flow channel. The manifold has a dam which allows liquid to pool in the manifold and allows for the creation of an airspace in the manifold, which reduces hydrostatic pressure inside the apparatus and enables the stacking of multiple cell culture units. Embodiments include cascading manifolds, manifolds having reservoirs, and manifolds having valves.
    Type: Grant
    Filed: August 30, 2017
    Date of Patent: June 11, 2019
    Assignee: Corning Incorporated
    Inventors: David Alan Kenney, Gregory Roger Martin, Allison Jean Tanner, Joseph Christopher Wall
  • Patent number: 10316298
    Abstract: Methods and compositions for producing fatty acid derivatives, for example, fatty esters, and commercial fuel compositions comprising fatty acid derivatives are described.
    Type: Grant
    Filed: May 11, 2015
    Date of Patent: June 11, 2019
    Assignee: REG Life Sciences, LLC
    Inventor: Alfred Gaertner
  • Patent number: 10265392
    Abstract: The present invention pertains to a vaccine comprising non live Lawsonia intracellularis antigen and a pharmaceutically acceptable carrier for use in a method to reduce in a pig the shedding of Lawsonia intracellularis bacteria associated with subclinical infection with Lawsonia intracellularis, by systemic administration of the vaccine to the pig.
    Type: Grant
    Filed: February 3, 2016
    Date of Patent: April 23, 2019
    Assignee: Intervet Inc.
    Inventor: Antonius Arnoldus Christiaan Jacobs
  • Patent number: 10238694
    Abstract: Species of human-derived bacteria belonging to the Clostridia class have been shown to induce accumulation of regulatory T cells (Treg cells) in the colon and suppress immune functions. Pharmaceutical compositions containing these bacteria can be used to prevent and treat immune-mediated diseases such as autoimmune diseases.
    Type: Grant
    Filed: November 29, 2012
    Date of Patent: March 26, 2019
    Assignees: The University of Tokyo, School Corporation, Azabu Veterinary Medicine Educational Institution
    Inventors: Kenya Honda, Koji Atarashi, Takeshi Tanoue, Masahira Hattori, Hidetoshi Morita
  • Patent number: 10214713
    Abstract: The current is directed to a device comprising, a reaction chamber where a reaction takes place, one or more feeding chambers which contain feeding solution comprising chemicals required for the reaction, and one or more porous membranes separating the reaction chamber from the one or more feeding chambers, and wherein the one or more porous membranes are in substantially vertical position. The current invention is also directed to an apparatus comprising a plurality of devices, wherein each device comprises of a reaction chamber where a reaction takes place, one or more feeding chambers which contain feeding solution comprising chemicals required for the reaction, and one or more porous membranes which separate the reaction chamber from feeding chambers, wherein the one or more porous membranes are in substantially vertical position. These devices and apparatuses can be used for high throughput synthesis of biomolecules and chemicals and biological screening assays.
    Type: Grant
    Filed: July 17, 2014
    Date of Patent: February 26, 2019
    Assignee: UNIVERSITY OF FLORIDA RESEARCH FOUNDATION, INCORPORATED
    Inventors: Zhonghui Hugh Fan, Kirsten Marie Jackson
  • Patent number: 10196609
    Abstract: The present invention provides a composition for promoting cardiac differentiation of a pluripotent stem cell containing an EGFR inhibitor. The present invention also provides a kit for promoting cardiac differentiation containing an EGFR inhibitor and a method for inducing cardiac differentiation of a pluripotent stem cell comprising culturing the pluripotent stem cell in a medium containing an EGFR inhibitor.
    Type: Grant
    Filed: February 5, 2014
    Date of Patent: February 5, 2019
    Assignee: KYOTO UNIVERSITY
    Inventors: Norio Nakatsuji, Itsunari Minami
  • Patent number: 10166258
    Abstract: This document provides methods and materials relating to platelet lysates. For example, methods and materials for using platelet lysate compositions to grow adult stem cells, to differentiate adult stem cells, to grow primary cell cultures, to grow tumor stem cells, and to identify effective growth factors are provided.
    Type: Grant
    Filed: December 3, 2015
    Date of Patent: January 1, 2019
    Assignee: Mayo Foundation for Medical Education and Research
    Inventors: Allan B. Dietz, Michael P. Gustafson, Greg W. Butler
  • Patent number: 10151004
    Abstract: A method for specifically detecting and optionally for quantifying Mycobacterium avium ssp. paratuberculosis (MAP) in a sample of an individual is provided. For this purpose, the detection of the presence of the IS900 region in a sample is performed by nucleic acid amplification using specific oligonucleotides. In a further aspect, a test kit for specifically detecting MAP in a sample by amplification methods is also provided. Finally, specific oligonucleotides that are suitable for specifically detecting MAP are described.
    Type: Grant
    Filed: April 26, 2013
    Date of Patent: December 11, 2018
    Assignee: Georg-August-Universität Göttingen Stiftung Öffentlichen Rechts
    Inventors: Claus-Peter Czerny, Pia Muenster
  • Patent number: 10138284
    Abstract: An insulin analog comprises a B-chain polypeptide containing a cyclohexanylalanine substitution at position B24 and optionally containing additional amino-acid substitutions at positions A8, B28, and/or B29. A proinsulin analog or single-chain insulin analog containing a B domain containing a cyclohexanylalanine substitution at position B24 and optionally containing additional amino-acid substitutions at positions A8, B28, and/or B29. The analog may be an analog of a mammalian insulin, such as human insulin. A nucleic acid encoding such an insulin analog is also provided. A method of lowering the blood sugar of a patient comprises administering a physiologically effective amount of the insulin analog or a physiologically acceptable salt thereof to a patient. A method of semi-synthesis using an unprotected octapeptide by means of modification of an endogenous tryptic site by non-standard amino-acid substitutions.
    Type: Grant
    Filed: September 27, 2016
    Date of Patent: November 27, 2018
    Assignee: Case Western Reserve University
    Inventor: Michael A. Weiss
  • Patent number: 10124027
    Abstract: The present invention provides methods of designing panels of bacteriophages as therapeutic compositions against bacterial infections. The present invention also provides panels of bacteriophages for use in the prevention or treatment of bacterial infections.
    Type: Grant
    Filed: May 3, 2013
    Date of Patent: November 13, 2018
    Assignee: BIOCONTROL LIMITED
    Inventors: David Harper, Katy Blake
  • Patent number: 10058578
    Abstract: Species of human-derived bacteria belonging to the Clostridia class have been shown to induce accumulation of regulatory T cells (Treg cells) in the colon and suppress immune functions. Pharmaceutical compositions containing these bacteria can be used to prevent and treat immune-mediated diseases such as autoimmune diseases.
    Type: Grant
    Filed: April 14, 2017
    Date of Patent: August 28, 2018
    Assignees: The University of Tokyo, School Corporation, Azabu Veterinary Medicine Educational Institution
    Inventors: Kenya Honda, Koji Atarashi, Takeshi Tanoue, Masahira Hattori, Hidetoshi Morita
  • Patent number: 10011812
    Abstract: This invention provides a culture control method and a cell culture apparatus that enable stable cell culture by regulating shear stress to be applied to cells within an adequate range. With the application of such culture control method and cell culture apparatus, cell culture is performed under agitation culture conditions in which the shear stress distribution is 0.5 Pa to 20 Pa in 80% or more of the culture vessel by volume.
    Type: Grant
    Filed: December 17, 2013
    Date of Patent: July 3, 2018
    Assignee: HITACHI, LTD.
    Inventors: Keisuke Shibuya, Ryoichi Haga, Masaru Namba, Kenichiro Oka, Ken Amano
  • Patent number: 10006055
    Abstract: The invention provides non-naturally occurring microbial organisms having a butadiene or crotyl alcohol pathway. The invention additionally provides methods of using such organisms to produce butadiene or crotyl alcohol.
    Type: Grant
    Filed: September 29, 2015
    Date of Patent: June 26, 2018
    Assignee: Genomatica, Inc.
    Inventors: Mark J. Burk, Anthony P. Burgard, Robin E. Osterhout, Jun Sun, Priti Pharkya
  • Patent number: 9938536
    Abstract: Methods and materials for modulating aluminum tolerance in plants are disclosed. For example, nucleic acids encoding aluminum tolerance-modulating polypeptides are disclosed as well as methods for using such nucleic acids to transform plant cells. Also disclosed are plants having increased tolerance to aluminum and methods of increasing plant yield in soil containing elevated levels of aluminum.
    Type: Grant
    Filed: November 1, 2012
    Date of Patent: April 10, 2018
    Assignee: Ceres, Inc.
    Inventors: Julissa Sosa, Wuyi Wang
  • Patent number: 9914758
    Abstract: Expression vector systems are provided for increased production of a recombinant GDF-5 (rhGDF-5) protein. Also provided are transformed host cells that were engineered to produce and express high levels of rhGDF-5 protein. Methods for production and high expression of rhGDF-5 protein are disclosed herein. The methods of enhancing production and protein expression of rhGDF-5 protein as disclosed are cost-effective, time-saving and are of manufacturing quality.
    Type: Grant
    Filed: December 22, 2016
    Date of Patent: March 13, 2018
    Assignee: Warsaw Orthopedic, Inc.
    Inventors: David S. Scher, Roger E. Harrington
  • Patent number: 9896655
    Abstract: The present invention relates compounds for stabilizing cells and methods of their use.
    Type: Grant
    Filed: April 14, 2016
    Date of Patent: February 20, 2018
    Assignee: The Scripps Research Institute
    Inventors: Yue Xu, Sheng Ding
  • Patent number: 9879057
    Abstract: Described are methods and products useful for identifying subjects with Mycobacterium avium subspecies paratuberculosis (MAP). A number of protein antigens secreted into culture filtrate by MAP are identified and binding proteins selective for these antigens are demonstrated to be useful for detecting subjects with MAP infections including subjects with Johne's disease.
    Type: Grant
    Filed: September 24, 2014
    Date of Patent: January 30, 2018
    Assignee: University of Guelph
    Inventors: Lucy M. Mutharia, Antonio Facciuolo
  • Patent number: 9790464
    Abstract: The present invention relates to a cell culture medium for culturing human cells comprising an anti-coagulated total blood material wherein the hemoglobin level is from about 8 to about 16 g/dl. More particularly, the invention provides a cell culture medium in which cells present in the blood are disrupted and the insoluble remnants of the lysated cells are removed. Further, the invention provides a method for the preparation of a cell culture medium for culturing human cells, according to the invention.
    Type: Grant
    Filed: September 24, 2010
    Date of Patent: October 17, 2017
    Assignee: BioMarker Kft.
    Inventors: Henriette Skribek, László Székely
  • Patent number: 9776931
    Abstract: A facility secondary NO3? salinized soil modifier and a modification method are disclosed. The present invention involves the preparation processes of two biological soil modifiers A and B and application methods thereof, which are specifically described as follows: (1) soil modifier A: solid, obtained by microbial fermentation with straw powder and rice bran and the like as raw materials, C/N between 30-50, applied by uniformly mixing at 1-1.4‰ by mass with facility ploughing soil, co-culturing at 35-50° C. for 5-7 days, and then returning to fields; and (2) soil modifier B: liquid, subjected to salt tolerance acclimation to have effective microbial populations, and used in the crop growth period. Based on the microbial techniques, the present invention can improve soil structureness, enhance microbial activity and promote biological immobilization on inorganic nitrogen in soils, significantly increasing the facility cultivation crop yield, and improving the product quality.
    Type: Grant
    Filed: June 19, 2014
    Date of Patent: October 3, 2017
    Assignee: Hohai University
    Inventors: Tingting Chang, Xiaohou Shao, Huilian Xu, Weina Wang, Lihua Chen, Xinyu Mao, Chunfang Wang
  • Patent number: 9765294
    Abstract: Chemically defined media for the industrial-scale culture of Bordetella species.
    Type: Grant
    Filed: September 11, 2014
    Date of Patent: September 19, 2017
    Assignee: GLAXOSMITHKLINE BIOLOGICALS S.A.
    Inventors: Philippe Marc Helene Dehottay, Philippe Goffin, Filipe Branco dos Santos, Bas Teusink
  • Patent number: 9751930
    Abstract: The present invention provides method for purifying a recombinant protein from a gram-negative bacterial host cell sample or extract thereof wherein said host cell expresses a recombinant protein and a recombinant disulphide isomerase DsbC; comprising: a. adjusting the pH of the host cell sample or extract thereof to a pH of 5 or less to precipitate the recombinant disulphide isomerase; and b. separating precipitated recombinant disulphide isomerase DsbC from the recombinant protein to produce a recombinant protein sample.
    Type: Grant
    Filed: July 27, 2011
    Date of Patent: September 5, 2017
    Assignee: UCB PHARMA, S.A.
    Inventor: Gavin Barry Wild
  • Patent number: 9689008
    Abstract: A fermentation method for producing coenzyme Q10 is provided, including stepwisly culturing of microbial strains capable of producing coenzyme Q10, wherein key promoting factors are added in each stage of culture, and in the stage of culture in a fermentor, dissolved oxygen feedback-fed batch culture technique is adopted to realize the feedback regulation of the production of coenzyme Q10, so as to improve the yield of coenzyme Q10.
    Type: Grant
    Filed: August 10, 2011
    Date of Patent: June 27, 2017
    Assignees: Inner Mongolia Kingdomway Pharmaceutical Limited, Xiamen Kingdomway Group Company
    Inventors: Yi Wu, Biqin Chen, Guanghuang Zhan, Youpao Zhu
  • Patent number: 9688959
    Abstract: In certain embodiments, this disclosure describes compositions comprising platelet lysates depleted of fibrinogen. In a further embodiment, the composition further comprises a cell culture medium component. This disclosure also provides a method for preparing the composition, comprising the steps of (a) lysing platelets providing a lysate; (b) removing cell debris; and (c) depleting fibrinogen by forming a removable mass by adding a metal salt such as calcium chloride. Furthermore, the disclosure also describes the product produced using said method.
    Type: Grant
    Filed: June 26, 2012
    Date of Patent: June 27, 2017
    Assignees: Emory University, Children's Healthcare of Atlanta, Inc.
    Inventors: Ian B. Copland, Jacques Galipeau
  • Patent number: 9676872
    Abstract: The present invention pertains to an affinity tag system for the immobilization and/or purification of molecules such as biological or organic molecules. The invention provides EF-hand subdomains of calcium binding proteins, such as calbindin D9k, as affinity tags and affinity ligands for immobilizing, detecting and/or for purifying molecules, particularly proteins. Also provided are methods utilizing the affinity tag system of the invention, affinity matrices comprising EF-hand subdomain affinity ligands and fusion proteins comprising EF-hand subdomain affinity tags.
    Type: Grant
    Filed: January 7, 2013
    Date of Patent: June 13, 2017
    Assignee: Northwestern University
    Inventors: David O'Connell, Sara Linse, Eva Thulin, Alejandro Merino
  • Patent number: 9663804
    Abstract: The present invention relates to the use of glycosyl hydrolase family 61 polypeptides in the presence of cutinases for polyester textile manufacture as well as a textile composition comprising glycosyl hydrolase family 61 polypeptides and cutinases.
    Type: Grant
    Filed: July 18, 2013
    Date of Patent: May 30, 2017
    Assignee: Novozymes A/S
    Inventors: Weijian Lai, Ting Sun, Leonardo De Maria
  • Patent number: 9649343
    Abstract: The present invention provides compositions that include an extract of human feces, and methods for using such compositions, including methods for replacing or supplementing or modifying a subject's colon microbiota, and methods for treating a disease, pathological condition, and/or iatrogenic condition of the colon.
    Type: Grant
    Filed: September 9, 2016
    Date of Patent: May 16, 2017
    Assignee: National Institutes of Health (NIH); U.S. Department of Health and Human Services (DHHS); The United States of America, NIH Division of Extramural Inventions and Tehnology Resources (DEITR)
    Inventors: Michael J. Sadowsky, Alexander Khoruts, Alexa R. Weingarden, Matthew J. Hamilton
  • Patent number: 9648813
    Abstract: The invention provides isolated polynucleotides comprising sequences encoding a u ORF peptides and variants and fragments thereof. The invention also provides constructs and vectors containing the polynucleotides. The invention further provides cells, plant cells and plants transformed with the polynucleotides and constructs. The invention also provides methods of using the polynucleotides to control expression of operably linked polynucleotides. The invention also provides methods of manipulating GDP-L-Galactose phosphorylase (GGP) expression and ascorbate production in plants utilizing the polynucleotides of the invention.
    Type: Grant
    Filed: December 20, 2013
    Date of Patent: May 16, 2017
    Assignee: The New Zealand Institute for Plant and Food Research Limited
    Inventors: William Alister Laing, Roger Paul Hellens, Richard Colin Macknight, Sean Michael Winsley Bulley