Micro-organism, Per Se (e.g., Protozoa, Etc.); Compositions Thereof; Proces Of Propagating, Maintaining Or Preserving Micro-organisms Or Compositions Thereof; Process Of Preparing Or Isolating A Composition Containing A Micro-organism; Culture Media Therefor Patents (Class 435/243)
  • Patent number: 11944675
    Abstract: The present invention is directed to a bioconjugate vaccine, such as an O 1-bioconjugate vaccine, comprising: a protein carrier comprising a protein carrier containing at least one consensus sequence, DIE-X-N-Z-S/T, wherein X and Z may be any natural amino acid except proline; at least one antigenic polysaccharide from at least one pathogenic bacterium, linked to the protein carrier; and, optionally, an adjuvant. In another aspect, the present invention is directed to a method of producing an O 1-bioconjugate in a bioreactor comprising a number steps.
    Type: Grant
    Filed: October 2, 2020
    Date of Patent: April 2, 2024
    Inventors: Fabiana Fernandez, Michael Kowarik, Michael Wacker, Michael Wetter
  • Patent number: 11932845
    Abstract: The invention provides non-naturally occurring microbial organisms having a 4-hydroxybutyrate, 1,4-butanediol, or other product pathway and being capable of producing 4-hydroxybutyrate, 1,4-butanediol, or other product, wherein the microbial organism comprises one or more genetic modifications. The invention additionally provides methods of producing 4-hydroxybutyrate, 1,4-butanediol, or other product or related products using the microbial organisms.
    Type: Grant
    Filed: June 3, 2013
    Date of Patent: March 19, 2024
    Assignee: Genomatica, Inc.
    Inventors: Priti Pharkya, Anthony P. Burgard, Stephen J. Van Dien, Robin E. Osterhout, Mark J. Burk, John D. Trawick, Michael P. Kuchinskas, Brian Steer
  • Patent number: 11920196
    Abstract: A method comprising obtaining a substantially cell-free sample of blood plasma or blood serum from a subject with osteoarthritis; and detecting a presence of or measuring a level of novel_miRNA_1 (gucuggcucaggguuggg) (SEQ ID NO: 1), novel_miRNA_2 (ucccuguucgggcgccacu) (SEQ ID NO: 2), novel_miRNA_3 (uguuuagcauccuguagccugc) (SEQ ID NO: 3), and novel_miRNA_4 (uaguggguuaucagaacu) (SEQ ID NO: 4). Also provided are methods where additional miRNAs are detected including novel miRNA 5 (SEQ ID NO: 5), novel miRNA 6 (SEQ ID NO: 6), novel miRNA 7 (SEQ ID NO: 7), novel miRNA 8 (SEQ ID NO: 8), novel miRNA 9 (SEQ ID NO: 9), novel miRNA 10 (SEQ ID NO: 10), novel miRNA 11 (SEQ ID NO: 11), novel miRNA 12 (SEQ ID NO: 12), novel miRNA 13 (SEQ ID NO: 13), hsa-miR-335-3p, hsa-miR-199a-5p, hsa-miR-671-3p, hsa-miR-1260b, hsa-miR-191-3p, hsa-miR-335-5p and/or hsa-miR-543.
    Type: Grant
    Filed: June 2, 2021
    Date of Patent: March 5, 2024
    Assignee: University Health Network
    Inventors: Mohit Kapoor, Rajiv Gandhi, Shabana Amanda Ali
  • Patent number: 11919058
    Abstract: The invention relates to a method for microbial remediation of underground water petroleum hydrocarbon contamination by regulating soil buffer capability, which comprises detecting the soil particle size of contaminated site soil, dividing the contaminated site soil into coarse-grained soil and fine-grained soil; dividing the contaminated site soil into high buffer capacity soil and low buffer capacity soil; and adjusting the composition and ratio of a biostimulant solution added to the contaminated site soil based on the classification of the contaminated site soil. The detecting step includes classifying soil with a particle size between 0.075 mm and 60 mm and a mass greater than or equal to 50% of the total mass as coarse-grained soil; and classifying soil with a particle size not greater than 0.075 mm and a mass greater than or equal to 50% as fine-grained soil.
    Type: Grant
    Filed: May 31, 2023
    Date of Patent: March 5, 2024
    Inventors: Shengyan Pu, Peng Wang, Shibin Liu, Xin Wang, Hui Ma, Pei An
  • Patent number: 11905503
    Abstract: Generation of energy and storage of energy for subsequent use is provided by electromethanogenesis of carbon dioxide into a fuel gas and the storage of the fuel gas for subsequent use. An electromethanogenic reactor includes an anode conductor and a cathode conductor wherein the cathode conductor includes submicron to micron scale pores. Electromethanogenesis microbes and/or enzymes are located in the micron scale pores of the cathode electrode conductor. Carbon dioxide is introduced into the electromethanogenic reactor and the electromethanogenesis microbes/enzymes and the carbon dioxide interact and produce a fuel gas. The fuel gas is stored for subsequent use, for example use in power generation.
    Type: Grant
    Filed: August 3, 2021
    Date of Patent: February 20, 2024
    Assignee: Lawrence Livermore National Security, LLC
    Inventors: Jennifer Marie Knipe, Sarah E. Baker, Marcus A. Worsley, Swetha Chandrasekaran
  • Patent number: 11898190
    Abstract: A method for producing a microbial oil includes steps of: genetically modifying a labyrinthulid by disrupting and/or silencing a gene, or by transforming another gene in addition to the disruption and/or gene silencing of the gene, and culturing the labyrinthulid, such that a fatty acid composition accumulated in the labyrinthulid comprises an increased EPA content; and collecting the microbial oil having the increased EPA content from the labyrinthulid. The labyrinthulid before the modification is selected from (A) a labyrinthulid belonging to the genus Parietichytrium or genus Schizochytrium and having very weak or no activity of producing PUFAs via a PUFA-PKS pathway; and (B) a labyrinthulid belonging to the genus Thraustochytrium in which a host PUFA-PKS gene is disrupted or silenced to a very weak level. The increased EPA content is preferably not less than 11.5% of a total fatty acid composition.
    Type: Grant
    Filed: August 13, 2021
    Date of Patent: February 13, 2024
    Inventors: Yuji Okita, Makoto Ito, Rie Hamaguchi, Hatsumi Goda, Seiya Mochinaga, Daisuke Honda
  • Patent number: 11884912
    Abstract: A cell culture device includes a culture unit, a gas supply unit, a first pressure unit, at least one inspecting unit and a control unit. The culture unit contains a cell culture liquid. The gas supply unit, connected with the culture unit, is used for transmitting a culture gas into the culture unit. The first pressure unit, connected with the culture unit, is used for applying a pressure to the cell culture liquid in the culture unit. The at least one inspecting unit, connected with the culture unit, is used for receiving the cell culture liquid for inspection. The control unit, electrically coupled with the culture unit, the first pressure unit, the gas supply unit and the at least one inspecting unit, is used for monitoring corresponding condition parameters to determine respective operations. In addition, a cell culture method for the cell culture device is also provided.
    Type: Grant
    Filed: December 26, 2019
    Date of Patent: January 30, 2024
    Inventors: Kuo-Hsing Wen, Ting-Hsuan Chen, Cheng-Tai Chen, Chien-An Chen, Su-Fung Chiu, Yung-Chi Chang, Nien-Jen Chou, Ping-Jung Wu, Shaw-Hwa Parng, Pei-Shin Jiang
  • Patent number: 11821029
    Abstract: The invention is directed to compositions and methods for collecting, transporting, and storing, without refrigeration, biological materials, which may comprise samples of biological, clinical, forensic, and/or environmental origin. These compositions preserve the viability of the collected organisms and/or the RNA/DNA and proteins in the sample composition mixture and permit the long-term storage of samples. Compositions are compatible with subsequent manipulation of the sample, including propagation and culture of the collected microorganisms, or isolation, purification, detection, and characterization of proteins, nucleic acids, and other macromolecules.
    Type: Grant
    Filed: September 2, 2020
    Date of Patent: November 21, 2023
    Assignee: Longhorn Vaccines and Diagnostics, LLC
    Inventors: Luke T. Daum, Gerald W. Fischer
  • Patent number: 11820686
    Abstract: A bioelectrochemical reactor (1) has an anode chamber (11) having at least two bioanodes (12, 13), and an anodic electrolyte (14) with an anodic electroactive microorganisms,—a cathode chamber (21) with at least one biocathode (22), and a cathodic electrolyte (24) with a cathodic electroactive microorganisms. The anode chamber (11) is separated from the cathode chamber (21) by, running from the anode chamber to the cathode chamber, a cation exchange membrane (31) and an anion exchange membrane (32). The cation and anion exchange membranes are separated from each other by an inter-membrane chamber (30), and means for applying a potential difference between the interconnected bioanodes and the biocathode/biocathodes. The bioanodes and biocathode/biocathodes have active surfaces such that the total active surface of the biocathode/biocathodes (22) is greater than the total active surface of the two bioanodes (12, 13).
    Type: Grant
    Filed: September 12, 2019
    Date of Patent: November 21, 2023
    Inventors: Sylvain Moreau, Théodore Bouchez, Jianghao Tian, Elie Le Quemener, Alain Bergel, Elise Blanchet, Benjamin Erable, Luc Etcheverry, Alain Huyard, Pierre Mauricrace, Nicolas Bernet, Eric Trably
  • Patent number: 11813323
    Abstract: The invention relates to an immunization regimen whereby an infant is protected against respiratory syncytial virus (RSV) through administration of a first anti-RSV immune response inducing composition to his or her mother during pregnancy, followed by administration of a second anti-RSV immune response inducing composition to the infant after birth.
    Type: Grant
    Filed: June 24, 2022
    Date of Patent: November 14, 2023
    Inventor: Philip Dormitzer
  • Patent number: 11807814
    Abstract: Disclosed herein is a method and system for converting biomass to biofuel, comprising a reaction apparatus including: a reaction tank configured to hold a process fluid; at least one mechanical rotating device comprising: a submergible chamber configured to operate within process, the submergible chamber having a first section including a first rotatable member and configured to receive biomass feedstock; a second section including a second rotatable member and configured to process biomass feedstock; and a third section including a third rotatable member and configured to treat the processed biomass feedstock effective to convert the processed biomass feedstock; a shaft in operable communication with each of the first, second, and third rotatable members for rotating said rotatable members about an axis; and a drive source for driving the shaft about said axis. Also disclosed herein are kits and methods for using the disclosed system to produce biofuel.
    Type: Grant
    Filed: May 11, 2021
    Date of Patent: November 7, 2023
    Inventor: Anders Mokvist
  • Patent number: 11802277
    Abstract: Thermostable Cas9 nucleases. The present invention relates to the field of genetic engineering and more particularly to nucleic acid editing and genome modification. The present invention provides in isolated Cas protein or polypeptide fragment thereof having an amino acid sequence of SEQ ID NO: 1 or a sequence of at least 77% identity therewith, wherein the Cas protein or polypeptide is capable of DNA cleavage at a temperature in the range 50° C. and 100° C. inclusive. The invention further provides isolated nucleic acid molecules encoding said Cas9 nucleases, expression vectors and host cells. The Cas9 nucleases disclosed herein provide novel tools for genetic engineering at elevated temperatures and are of particular value in the genetic manipulation of thermophilic organisms; particularly microorganisms.
    Type: Grant
    Filed: June 19, 2020
    Date of Patent: October 31, 2023
    Assignee: Wageningen Universiteit
    Inventors: John Van Der Oost, Martinus Johannes Arnoldus Daas, Servatius Wilhelmus Maria Kengen, Willem Meindert De Vos
  • Patent number: 11793197
    Abstract: Use of the molecule with the following formula (I) for its fungicidal and/or bactericidal activity on fungi, oomycetes and/or pathogenic bacteria of plants and crop seeds.
    Type: Grant
    Filed: February 8, 2022
    Date of Patent: October 24, 2023
    Inventors: Yann Thomas, Odon Thiebeauld
  • Patent number: 11788055
    Abstract: Methods for increasing carbon-based chemical product yield in an organism by perturbing redox balance in an organism as well as nonnaturally occurring organisms with perturbed redox balance and methods for their use in producing carbon-based chemical products are provided.
    Type: Grant
    Filed: April 30, 2019
    Date of Patent: October 17, 2023
    Inventors: Alexander Brett Foster, Jonathan Paul Combe, Arghya Barman
  • Patent number: 11787875
    Abstract: Disclosed are materials and methods for improved single chain variable fragments.
    Type: Grant
    Filed: August 14, 2020
    Date of Patent: October 17, 2023
    Assignee: Janssen Biotech, Inc.
    Inventors: Jinquan Luo, Lauren Boucher, Michael Feldkamp, Michael Diem, Anthony A. Armstrong, Alexey Teplyakov, Chichi Huang
  • Patent number: 11781105
    Abstract: A system for quickly determining antibiotic sensitivity of a microorganism comprising a triangular-shaped plate and cover for culturing, recovering, and re-suspending recovered microbial colonies and a second triangular-shaped plate comprising a non-liquid medium cut with concentric trenches over which the re-suspended microbial colonies are distributed by centrifugation and contacted with antimicrobial strips or disks.
    Type: Grant
    Filed: June 5, 2020
    Date of Patent: October 10, 2023
    Assignees: National Guard Health Affairs, King Saud bin Abdulaziz University for Health Sciences, King Abdullah International Medical Research Center
    Inventor: Ahmad Mohammed Shikan Aljefri
  • Patent number: 11767235
    Abstract: A vacuum airlift system for treating an aqueous effluent includes an upflow liquid portion, where the upflow liquid portion is configured to retain a fluid, and a fluid inlet, the fluid inlet being fluidly coupled with the upflow liquid portion, where the fluid inlet is positioned at about a bottom of the upflow liquid portion. The vacuum airlift system can also include a downflow liquid portion, where the downflow liquid portion is fluidly coupled with the upflow liquid portion, and a fluid outlet, the fluid outlet being fluidly coupled with the downflow liquid portion, where the fluid outlet is positioned at about a bottom of the downflow liquid portion. The vacuum airlift system can also include a plurality of aerators fed by one or more fluidic oscillators, the plurality of aerators being coupled to the upflow liquid column.
    Type: Grant
    Filed: October 7, 2021
    Date of Patent: September 26, 2023
    Assignee: Searen, LLC
    Inventors: Thomas Wood Andrews, Emmanuel Pierre Pascal Briquet, John Rodgers Brooks, Jr.
  • Patent number: 11746134
    Abstract: The present disclosure relates to a human FGF21 mutant with improved effectiveness and stability, preparation method and pharmaceutical composition comprising the mutant, and a use thereof, and specifically relates to a human fibroblast growth factor 21 (FGF21) mutant, a gene encoding the same, and a method for preparing the mutant and a method of using the mutant for treating type 2 diabetes, obesity, dyslipidemia, or metabolic disorders.
    Type: Grant
    Filed: April 11, 2018
    Date of Patent: September 5, 2023
    Assignee: Heifei Zhongke Longwood Biotechnology Co., Ltd.
    Inventors: Junfeng Wang, Hongxin Zhao, Lei Zhu
  • Patent number: 11725221
    Abstract: This invention relates to a method of using a two-step serial centrifugation process in extracting nutritive oil from a fermentation broth, this novel method prevents oil yield losses while preserving product quality.
    Type: Grant
    Filed: August 9, 2018
    Date of Patent: August 15, 2023
    Inventors: Neil Francis Leininger, Holger Pfeifer, David Allen Tinsley, Jochen Lebert, Marc Beiser
  • Patent number: 11702682
    Abstract: Provided is a method of producing and isolating a diamine produced by microbial fermentation that minimizes undesirable salt formation to provide a lower cost process.
    Type: Grant
    Filed: July 13, 2020
    Date of Patent: July 18, 2023
    Assignee: Genomatica, Inc.
    Inventors: Lauri H. Suominen, Connor J. Galleher, Michael Japs, Mark J. Burk, Cara Tracewell
  • Patent number: 11692164
    Abstract: Compositions are provided that contain sporulated coccidial oocysts, wherein the compositions are free of antibiotics and comprise formalin at a concentration sufficient to inhibit microbial growth for at least 12 months while maintaining oocyst viability. Methods of preparing such compositions are also provided.
    Type: Grant
    Filed: July 2, 2021
    Date of Patent: July 4, 2023
    Assignee: Huvepharma Inc.
    Inventors: Mary Ann Pfannenstiel, Jennifer Albrecht, Glen M. Wilkinson
  • Patent number: 11692033
    Abstract: The present invention relates to an isolated humanized protein binding to human Glycoprotein VI (hGPVI) for treating a GPVI-related condition in a subject in need thereof, wherein said isolated humanized protein is to be administered during at least 2 hours to the subject, preferably during at least 4 to 6 hours.
    Type: Grant
    Filed: February 2, 2018
    Date of Patent: July 4, 2023
    Assignees: Acticor Biotech, Université Paris Cité, Université Paris-XIII, Inserm (Institut National de la Santé et de la Recherche Médicale), Université Paris-Saclay
    Inventors: Philippe Billiald, Martine Jandrot-Perrus, Gilles Avenard
  • Patent number: 11661605
    Abstract: Provided is a method for intracellularly producing a large amount of a Cry protein in a Bacillus bacterium. A method for producing a Cry protein or a culture product comprising the Cry protein, comprising transforming a Bacillus bacterium with an expression plasmid incorporating a gene encoding the Cry protein operably linked to a regulatory region comprising a ?A-dependent promoter or a ?H-dependent promoter, and culturing the transformed cell, wherein the expression plasmid comprises a polynucleotide encoding a replication protein consisting of the amino acid sequence set forth in SEQ ID NO: 9 or a protein having an identity of 80% or more or more with the amino acid sequence of the replication protein and involved in replication initiation.
    Type: Grant
    Filed: January 11, 2019
    Date of Patent: May 30, 2023
    Assignee: Kao Corporation
    Inventors: Shenghao Liu, Yasushi Kageyama, Mika Terai
  • Patent number: 11649298
    Abstract: The present invention provides process for treating crop kernels, comprising the steps of a) soaking kernels in water to produce soaked kernels; b) grinding the soaked kernels; c) treating the soaked kernels in the presence of an effective amount of GH62 polypeptide having arabinofuranosidase activity or a GH43 polypeptide having arabinofuranosidase activity, wherein step c) is performed before, during or after step b).
    Type: Grant
    Filed: October 1, 2020
    Date of Patent: May 16, 2023
    Assignee: Novozymes A/S
    Inventors: Yi Cao, James Lavigne, Bernardo Vidal, Jr., Thomas Patrick Gibbons, Chee-Leong Soong, Brian R. Scott, Randall Scott Deinhammer, Zhen Long, Michael John Akerman, Xinyu Shen, Yu Zhang
  • Patent number: 11623195
    Abstract: Provided are an apparatus and a method for reaction for use in a co-precipitation reaction for preparing a catalyst or a cathode active material for a lithium secondary battery, which injects a raw material (a solution) at least between impellers according to the solution level in a vessel, thereby making a stirring speed uniform and, in particular, minimizing a concentration difference between solutions. The apparatus for the reaction may comprise: a reaction vessel; a stirring means provided inside the reaction vessel and having multistage impellers; and a raw material injecting means, comprising at least one injection nozzle connected to the reaction vessel, for injecting a raw material at least between impellers.
    Type: Grant
    Filed: January 12, 2018
    Date of Patent: April 11, 2023
    Inventor: Ki-Sung You
  • Patent number: 11613565
    Abstract: Disclosed are peptides comprising amino acid residues of the dimer interface of human B7-1 and B7-2 and compositions and uses thereof.
    Type: Grant
    Filed: January 21, 2020
    Date of Patent: March 28, 2023
    Inventors: Raymond Kaempfer, Revital Levy
  • Patent number: 11603400
    Abstract: The present invention generally relates to methods of generating antibodies against a species of pathogen that involve identifying the pathogen that is most genetically representative of member of pathogen species and using the identified pathogen to generate an antibody.
    Type: Grant
    Filed: June 18, 2018
    Date of Patent: March 14, 2023
    Assignee: DNAE Group Holdings Limited
    Inventors: Colin Dykes, Sergey A. Dryga, Lisa-Jo Ann Clarizia, Eddie W. Adams, Meghan E. Norvell
  • Patent number: 11542321
    Abstract: This invention relates to engineered CH2 domain molecules containing amino acids in the framework regions that confer enhanced stability and/or solubility. In particular, the invention provides engineered CH2 domain molecules containing amino acid residues that differ from a wild type CH2 domain or a template CH2 domain molecule within one or more framework regions and that result in improved stability and/or solubility.
    Type: Grant
    Filed: March 20, 2018
    Date of Patent: January 3, 2023
    Assignee: Research Corporation Technologies, Inc.
    Inventor: Kurt R. Gehlsen
  • Patent number: 11534722
    Abstract: A gas separation apparatus includes a separation membrane module including at least one gas separation membrane element in a housing, a casing for blocking external air, and a heat source unit for adjusting a temperature of a heat medium with which the casing is filled. The casing holds greater than or equal to two separation membrane modules.
    Type: Grant
    Filed: October 25, 2018
    Date of Patent: December 27, 2022
    Inventors: Nobutaka Kodama, Takehiro Nakasuji, Osamu Okada, Masaaki Teramoto, Nobuaki Hanai
  • Patent number: 11530426
    Abstract: The disclosure provides a method for biohydrogen production. The method includes: mixing a hydrogen production medium and a buffer solution Na2HPO4/NaH2PO4 having a pH value of 5-9, to yield a first mixture; adding corn stalk powder and cellulase to the first mixture and mixing, to yield a second mixture; adding a suspension of photosynthesis bacteria HAU-M1 at the late exponential phase to the second mixture, to yield a third mixture; and sealing the third mixture and allowing for photo-fermentation biohydrogen production under anaerobic fermentation conditions.
    Type: Grant
    Filed: April 16, 2021
    Date of Patent: December 20, 2022
    Inventors: Chaoyang Lu, Quanguo Zhang, Zhiping Zhang, Danping Jiang, Yanyan Jing, Yi Wang, Kaixin Wang, Siyi Guo, Jian Wang
  • Patent number: 11491233
    Abstract: Disclosed herein are functionalized hyaluronic acid (HA), a responsive elastic polymer system comprising functionalized HA, and methods of fabrication and utilization of the same. This polymer system may be used for controlled local or systemic drug delivery release of analgesics, anesthetics, antibiotics and other drugs as well as tissue engineering articles.
    Type: Grant
    Filed: June 13, 2017
    Date of Patent: November 8, 2022
    Assignee: Purdue Research Foundation
    Inventors: Meng Deng, Liangju Kuang
  • Patent number: 11477989
    Abstract: The invention relates to a particulate product comprising at least 10 wt. % of the coated acetate particles having the following characteristics: (a) comprising one or more carrier particles containing at least 80 wt. % of sodium acetate component selected from sodium acetate hydrates, sodium acetate anhydrous and combinations thereof; (b) comprising a coating layer that covers the one or more carrier particles, said coating layer containing at least 60 wt. % of potassium acetate; (c) containing potassium and sodium in a molar ratio of 0.4:1 to 5:1; and (d) a particle size in the range of 40 to 1,000 ?m. The coated acetate particles in the particulate product are very stable, even though the potassium acetate in the coating layer is in direct contact with the surrounding atmosphere. The particulate product can suitably be used in the preparation of foodstuffs and beverages.
    Type: Grant
    Filed: May 6, 2020
    Date of Patent: October 25, 2022
    Assignee: PURAC BIOCHEM B.V.
    Inventors: Lambertus Henricus Elisabeth Roozen, Gerrit Anthon Rene Hilhorst, Heny Kusumawardani, Apostolos Papageorgiou, Kees Van Der Voort Maarschalk
  • Patent number: 11466269
    Abstract: The present invention relates to the field of molecular biology and cell biology. More specifically, the present invention relates to a CRISPR-Cas system for a Labyrinthulomycetes host cell.
    Type: Grant
    Filed: July 13, 2017
    Date of Patent: October 11, 2022
    Assignee: DSM IP ASSETS B.V.
    Inventors: James Casey Lippmeier, Yelena Betz, Johannes Andries Roubos, René Verwaal
  • Patent number: 11452773
    Abstract: The invention relates to an immunization regimen whereby an infant is protected against respiratory syncytial virus (RSV) through administration of a first anti-RSV immune response inducing composition to his or her mother during pregnancy, followed by administration of a second anti-RSV immune response inducing composition to the infant after birth.
    Type: Grant
    Filed: May 28, 2019
    Date of Patent: September 27, 2022
    Inventor: Philip Dormitzer
  • Patent number: 11447732
    Abstract: Cell culture systems and methods provide improved immunotherapeutic product manufacturing with greater scalability, flexibility, and automation. Cell culture systems are configured with interchangeable cartridges, allowing versatility and scalability. Systems are configured to have multiple connected cell culture chambers, which allows parallel processing of different types of cells. Gas-impermeable cell culture chambers and methods for generating cells in closed systems prevent contamination and user error. Methods for recycling cell culture medium provide additional efficiencies.
    Type: Grant
    Filed: October 20, 2020
    Date of Patent: September 20, 2022
    Assignee: FLASKWORKS, LLC
    Inventors: Shashi K. Murthy, Andrew Kozbial
  • Patent number: 11432575
    Abstract: Methods of production of edible filamentous fungal biomat formulations are provided as standalone protein sources and/or protein ingredients in foodstuffs as well as a one-time use or repeated use self-contained biomat reactor comprising a container with at least one compartment and placed within the compartment(s), a feedstock, a fungal inoculum, a gas-permeable membrane, and optionally a liquid nutrient medium.
    Type: Grant
    Filed: February 27, 2020
    Date of Patent: September 6, 2022
    Assignee: THE FYNDER GROUP, INC.
    Inventors: Richard Eugene Macur, Yuval Charles Avniel, Renata Usaite Black, Maximilian DeVane Hamilton, Michael John Harney, Eleanore Brophy Eckstrom, Mark Andrew Kozubal
  • Patent number: 11370816
    Abstract: Synthetic histatins composed of combinations of functional domains of natural histatins separated by exogenous linkers are described as are methods of using endogenous and synthetic histatins for the treatment of ocular diseases or conditions.
    Type: Grant
    Filed: September 3, 2020
    Date of Patent: June 28, 2022
    Assignee: The Board of Trustees of the University of Illinois
    Inventor: Vinay Aakalu
  • Patent number: 11338294
    Abstract: Toward forming a single hybrid biosensing-imaging system that can operate inside an incubator, structures and methods are directed to placing modular and removable biosensors and biocompatible interfaces in 3D transparent test wells that contain biological samples. The technology enables continuous monitoring of multiple simultaneous parameters and functions of a living cell or cell clusters such as alterations of cellular ligands, physicochemical biomarkers, phenotypes, and/or extracellular compositions upon interactions with analytes or during progressions. Methods of capturing and analyzing direct orthogonal information from biological samples in 2D and 3D, which are conducive to generating new insights are presented.
    Type: Grant
    Filed: October 25, 2019
    Date of Patent: May 24, 2022
    Assignee: Polybiomics, Inc.
    Inventors: Mandana Veiseh, Pirooz Parvarandeh, S. Bahram Bahrami, Oliver Peter King-Smith, Todd S. Rutherford, Aaron Peter Schellenberg, Timothy Scott Edward Hiller
  • Patent number: 11293046
    Abstract: The invention provides devices and methods for automatically preparing pure cultures of microbial cells under native environmental conditions. The devices and methods permit the discovery and investigation of novel microorganisms having unknown culture requirements from natural environments and microbiomes. Cultures obtained using the invention lead to the identification and isolation of novel biologically active compounds. The devices utilize a single entry pore in a thin membrane to select a single cell for clonal expansion.
    Type: Grant
    Filed: May 23, 2016
    Date of Patent: April 5, 2022
    Assignee: Northeastern University
    Inventor: Slava Epstein
  • Patent number: 11278027
    Abstract: Use of the molecule with the following formula (I) for its fungicidal and/or bactericidal activity on fungi, oomycetes and/or pathogenic bacteria of plants and crop seeds.
    Type: Grant
    Filed: June 8, 2018
    Date of Patent: March 22, 2022
    Assignee: Immunrise Biocontrol France
    Inventors: Yann Thomas, Odon Thiebeauld
  • Patent number: 11278609
    Abstract: Medicament for the treatment or the prevention of a bacterial infection, characterized in that it contains at least one polypeptide selected from the group of SEQ ID NO: 1 to SEQ ID NO: 9, and contiguous fragments thereof, wherein said at least one polypeptide or contiguous fragment thereof induces opsonic antibodies in a patient in need thereof. The polypeptides or the contiguous fragments thereof according to the present invention can be used for the preparation of a vaccine against an infection against Enterococcus.
    Type: Grant
    Filed: February 25, 2015
    Date of Patent: March 22, 2022
    Inventors: Johannes Hübner, Axel Hartke, Luis Felipe Romero Saavedra
  • Patent number: 11274321
    Abstract: Compositions and methods for a hybrid biological and chemical process that captures and converts carbon dioxide and/or other forms of inorganic carbon and/or CI carbon sources including but not limited to carbon monoxide, methane, methanol, formate, or formic acid, and/or mixtures containing CI chemicals including but not limited to various syngas compositions, into organic chemicals including biofuels or other valuable biomass, chemical, industrial, or pharmaceutical products are provided. The present invention, in certain embodiments, fixes inorganic carbon or CI carbon sources into longer carbon chain organic chemicals by utilizing microorganisms capable of performing the oxyhydrogen reaction and the autotrophic fixation of CO2 in one or more steps of the process.
    Type: Grant
    Filed: April 26, 2018
    Date of Patent: March 15, 2022
    Assignee: Kiverdi, Inc.
    Inventors: John S. Reed, Lisa Dyson
  • Patent number: 11248032
    Abstract: Provided are a method for producing a soluble recombinant human-basic fibroblast growth factor (rh-bFGF), a recombinant human-basic fibroblast growth factor (rh-bFGF) obtained by the method, and a mutated nucleic acid molecule encoding the recombinant human-basic fibroblast growth factor (rh-bFGF).
    Type: Grant
    Filed: June 23, 2017
    Date of Patent: February 15, 2022
    Inventors: Haizhou Fang, Bo Yang, La Ma, Peimin Dai, Xinzhi Wang, Malcolm Ngiam, Qi Xue, Yongjun Yang
  • Patent number: 11162120
    Abstract: Described is a method for the enzymatic production of an acyl phosphate from a 2-hydroxyaldehyde using a phosphoketolase or a sulfoacetaldehyde acetyltransferase.
    Type: Grant
    Filed: September 8, 2016
    Date of Patent: November 2, 2021
    Assignees: Scientist of Fortune, S.A., Global Bioenergies
    Inventor: Philippe Marlière
  • Patent number: 11154858
    Abstract: Disclosed herein are various bioreactor devices and systems for growing cellular material, and related methods of growing cellular material. In some cases, a system can include a bioreactor system having one or more wells with at least one fluidic passageway coupled to each well to feed fluids to biological material being developed inside the well. In some cases, a bioreactor system can include a main body comprising the perimeter of the well and the fluidic passageways, a cover that forms the top of the well and provides optical access into the well, and a base that forms the bottom surface of the well. The cover and base can be attached and detached from the main body to seal the well closed and to physically access the contents of the well.
    Type: Grant
    Filed: October 6, 2016
    Date of Patent: October 26, 2021
    Assignee: University of Pittsburgh—Of the Commonwealth System of Higher Education
    Inventors: Riccardo Gottardi, Peter Alexander, Bryan Romell, Rocky S. Tuan
  • Patent number: 11118197
    Abstract: In one aspect, the present invention provides methods for preparing a fermentation product. The methods include pre-treating a mixture of biomass and ionic liquid, wherein the ionic liquid comprises a choline cation and the biomass comprises polysaccharide and lignin. The methods further include forming hydrolysates from the introduction of glycoside hydrolase to the pre-treated mixture at conditions sufficient to produce a sugar composition mixture for fermentation steps. The present invention provides methods for loading biomass mixtures in a batch-fed process, wherein the biomass slurries can be loaded into water or a concentrated sugar composition for hydrolysate production. The methods can be performed in a one-pot process, wherein the ionic liquids are present in the mixtures throughout each step. Aspects of the invention provide compositions of sugar composition mixtures and fermentation product mixtures.
    Type: Grant
    Filed: November 21, 2016
    Date of Patent: September 14, 2021
    Assignee: National Technology & Engineering Solutions of Sandia, LLC
    Inventors: Feng Xu, Blake Simmons, Seema Singh
  • Patent number: 11053302
    Abstract: The present invention relates to single domain antibodies comprising at least one modification relative to the 4D5 antibody scaffold or human germline VH3 domain, the modifications selected from the group consisting of H35D, A78V, S93V, S93G and W103R, with the position numbering being according to the Kabat numbering scheme. Disulfide-free variants further comprise at least one additional modification selected from the group consisting of C22S, A24I, A24L and C92T, and with the proviso that at least one of C22S and C92T is present. Further encompassed are the multi-modular antibody molecules and antibody conjugates comprising single domain antibodies, as well as methods for producing them. The invention in particular provides a library of the single domain antibodies or multi-modular antibody molecules and a method for selecting an antibody that binds an antigen.
    Type: Grant
    Filed: November 4, 2015
    Date of Patent: July 6, 2021
    Assignee: Nanyang Technological University
    Inventors: Tobias Carl Cornvik, Ignacio Jose Asial, Par Lennart Nordlund
  • Patent number: 10996149
    Abstract: A device and a method for isolating a target from a biological sample are provided. The target is bound to solid phase substrate to form target bound solid phase substrate. The device includes a lower plate with an upper surface having a plurality of regions. The biological sample is receivable on a first of the regions. An upper plate has a lower surface directed to the upper surface of the lower plate. A force is positioned adjacent the upper plate and attracts the target bound solid phase substrate toward the lower surface of the upper plate. At least one of the upper plate and the lower plate is movable from a first position wherein the target bound solid phase substrate in the biological sample are drawn to the lower surface of the upper plate and a second position wherein the target bound solid phase substrate are isolated from the biological sample.
    Type: Grant
    Filed: August 30, 2017
    Date of Patent: May 4, 2021
    Assignee: Wisconsin Alumni Research Foundation
    Inventors: David J. Beebe, Ben P. Casavant, David J. Guckenberger, Scott M. Berry
  • Patent number: 10842870
    Abstract: The present invention relates to humanized anti-human GPVI antibodies and uses thereof.
    Type: Grant
    Filed: August 5, 2016
    Date of Patent: November 24, 2020
    Inventors: Philippe Billiald, Martine Jandrot-Perrus
  • Patent number: 10781417
    Abstract: An apparatus for processing cells is disclosed. In one embodiment, a fixed bed reactor is provided for the cells, the fixed bed reactor including a portion movable from a first position corresponding to a packed condition of the fixed bed to a second position corresponding to a depacked condition of the fixed bed. Movement of the partition facilitates harvesting of the cells there from. Related apparatus, kits, methods, and systems are also disclosed.
    Type: Grant
    Filed: December 11, 2013
    Date of Patent: September 22, 2020
    Inventors: Suresh Purushothaman, Jose Antonio Castillo Gonzalez, Jean-Christophe Drugmand, Vishwas Pethe, Derek Pendlebury, Fabien Moncaubeig