Preserving Or Maintaining Micro-organism Patents (Class 435/260)
  • Patent number: 4507387
    Abstract: A method of storing a quantity of red blood cells uses a blood bag which comprises a plastic polyvinyl chloride formulation which contains from 5 to 30 percent by weight of a first plasticizer material which is essentially nonextractable by blood plasma stored in the bag up to 35 days at about 4.degree. C.; and from 10 to 25 percent by weight of a second plasticizer which is significantly extracted by blood plasma stored in the bag up to 35 days at about 4.degree. C. and which, upon leaching, is capable of suppressing the formation of plasma hemoglobin.
    Type: Grant
    Filed: March 9, 1983
    Date of Patent: March 26, 1985
    Assignee: Baxter Travenol Laboratories, Inc.
    Inventors: Henry M. Gajewski, Barry H. Vernick, Paul E. Measells, Jan W. Garber
  • Patent number: 4472439
    Abstract: A heat treatment for live yeast cell slurries and pastes, which comprises heating the slurry or paste at a temperature of about 60.degree. C. or greater for a sufficient period of time, increases the fluidity of the slurry or paste and allows more highly concentrated suspensions to be processed than otherwise possible.
    Type: Grant
    Filed: January 7, 1980
    Date of Patent: September 18, 1984
    Assignee: Standard Oil Company (Indiana)
    Inventors: Cavit Akin, John A. Ridgway
  • Patent number: 4465772
    Abstract: Effective disinfection and washing of immobilized lactase from Aspergillus oryzae is carried out after a period of hydrolyzing lactose by treatment of the immobilized lactase with aqueous solutions of pH's 2 to 4 and 6 to 8.7, one or both of which contain a disinfectant. The solution having a pH of 6 to 8.7 may be a phosphate buffered solution. The lactase may be immobilized on a high polymer compound by covalent linkage. The immobilized lactase has outstanding resistance to chemicals and wide pH stability so that decrease of lactase activity is minimized.
    Type: Grant
    Filed: October 1, 1981
    Date of Patent: August 14, 1984
    Assignee: Sumitomo Chemical Company, Limited
    Inventors: Hideo Hirohara, Hidefumi Yamamoto, Emiko Kawano, Mamoru Hattori, Hisao Yamaguchi
  • Patent number: 4464470
    Abstract: A method and materials are provided for replication of virulent Treponema pallidum in tissue culture, employing a modified Eagle's minimum essential medium, wherein said in vitro cultivated T. pallidum can be utilized as a source of relatively pure organisms, free of host tissue, for the preparation of a vaccine against syphilis and as a source of organisms for use in specific immunological tests for syphilis.
    Type: Grant
    Filed: January 27, 1982
    Date of Patent: August 7, 1984
    Assignee: SRI International
    Inventors: A. Howard Fieldsteel, David L. Cox, Randolph A. Moeckli
  • Patent number: 4443538
    Abstract: Enzyme-containing cells immobilized in an alginate gel are stabilized by contacting the gel with glycerol in a ratio of cellstoglycerol of 2:1 to 1:5. The enzyme-containing cells preferably convert sucrose to isomaltulose.
    Type: Grant
    Filed: May 5, 1982
    Date of Patent: April 17, 1984
    Assignee: Tate & Lyle Public Limited Company
    Inventor: Peter S. J. Cheetham
  • Patent number: 4434231
    Abstract: Novel processes are provided for the embedding of microorganisms within a polymer matrix wherein the matrix comprises a polymer gel having a base of at least one polymer selected from the group of polysaccharides, in which said polymer is at least partially cross-linked. The embedded microorganism products are useful in the inoculation of leguminous and non-leguminous plants in order to increase their nitrogen-fixing potential and nutrition, respectively.
    Type: Grant
    Filed: August 14, 1981
    Date of Patent: February 28, 1984
    Assignee: Rhone-Poulenc Industries
    Inventor: Gerard Jung
  • Patent number: 4414228
    Abstract: The present invention relates to a process for preparing bread dough which may be deep-frozen with a view to long conservation thereof, characterized in that the yeast used was previously subjected to a stabilization by deep-freezing and that the mixing and kneading operations are carried out in a minimum of time without the dough attaining the temperature of 20.degree. C., after which the dough obtained is rapidly cooled until its innermost temperature attains at least -7.degree. C. The present invention also relates to a process for biological de-freezing of deep-frozen bread dough.
    Type: Grant
    Filed: December 30, 1981
    Date of Patent: November 8, 1983
    Assignee: Grandes Boulangeries Associees G.B.A.
    Inventor: Andre Nourigeon
  • Patent number: 4409245
    Abstract: Living cultures of microorganisms used in the preparation of foodstuffs by microbiological processing are protected against attack by bacteriophage viruses by the addition thereto of terpene. The terpene is added in an amount which is effective to obtain viricidal activity but ineffective to cause toxic effects on the microorganisms. The terpene is one obtainable from aromatic plants by steam distillation. Terpenes or mixtures of terpenes which have proved suitable are those obtained from black pepper oil, cinnamon flower oil, cardamon oil, linallyl acetate, cinnamic aldehyde, safrol, carvon and cis/ trans citral, used individually or mixed together. They may added dissolved in a carrier such as 1,2-propanediol. The terpenes demonstrate a viricidal activity in a concentration which is one or more powers of ten lower than the concentration at which the terpenes have toxic effects on the microorganisms.
    Type: Grant
    Filed: September 28, 1981
    Date of Patent: October 11, 1983
    Assignee: Chimicasa GmbH
    Inventors: Erich Wolf, Andreas Lembke, Rolf Deininger
  • Patent number: 4391887
    Abstract: A process for the production of a storage-stable preparation of micro-organisms, starting from a mixed culture of micro-organisms, which is capable to degrade products of industrial organic syntheses, which comprises that the culture conditions of an active mixed culture enriched in the customary manner are optimized, with the addition of the product to be degraded, in the customary manner according to the rate of degradation of the product and this mixed culture is converted in at least two passes into a stabilized activated mixed culture under the optimum conditions thus determined, and the culture thereby obtained is rendered storage-stable, its activity being maintained.
    Type: Grant
    Filed: August 13, 1981
    Date of Patent: July 5, 1983
    Assignee: Bayer Aktiengesellschaft
    Inventors: Jorg Baumgarten, Werner Frommer, Delf Schmidt, Friedrich Schmidt, Douglas M. Munnecke
  • Patent number: 4380582
    Abstract: A method of preparing a variola virus which comprises growing the virus on the embryos of chicken eggs, recoverng the inoculated embryos, stabilizing the recovered embryos with a mixture comprising; lactose, raffinose, lysine, sodium glutamate, dextrin, isoniazid and thiourea.
    Type: Grant
    Filed: July 9, 1965
    Date of Patent: April 19, 1983
    Assignee: The United States of America as represented by the Secretary of the Army
    Inventors: Michael D. Orlando, Jean M. Riley
  • Patent number: 4380552
    Abstract: Deacidifying wine by passage through an alginate gel containing living cells of Leuconostoc oenos therein. To ensure maximum viability, the alginate gel is stored in a resting medium, preferably sterile grape juice containing 5-12% ethanol. Before deacidifying wine the immobilized cells are conditioned to a wine milieu.
    Type: Grant
    Filed: October 27, 1980
    Date of Patent: April 19, 1983
    Assignee: Novo Industri A/S
    Inventors: Stina M. Gestrelius, Jorgen H. Kjaer
  • Patent number: 4374201
    Abstract: A method of coating a dry variola virus obtained by the inoculation of the mbryo of chicken eggs and stabilized by the addition of lactose, raffinose, lysine, sodium glutamate, isoniazid, and thiourea by treatment with methyl amyl acetate.
    Type: Grant
    Filed: July 9, 1965
    Date of Patent: February 15, 1983
    Assignee: The United States of America as represented by the Secretary of the Army
    Inventors: Michael D. Orlando, Jean M. Riley
  • Patent number: 4351904
    Abstract: A bacteria-containing composition for use in making cottage cheese is prepared containing a strain of Streptococcus diacetilactis that produces substantial amounts of diacetyl in a milk culture, a strain of diacetyl deficient mutant Streptococcus diacetilactis that produces essentially no diacetyl in a milk culture and a suitable carrier for maintaining viability of the Streptococcus diacetilactis. By the use of the two types of Streptococcus diacetilactis, a manufacturer of cottage cheese can optimize both the cell count to achieve the desired inhibition of spoilage bacteria and flavor production to achieve the desired flavor in the finished product.
    Type: Grant
    Filed: July 9, 1981
    Date of Patent: September 28, 1982
    Inventor: Edmond L. Sing
  • Patent number: 4335144
    Abstract: Porous granules of active yeast are produced by mixing moist yeast having a solids content of 30% to 40% with a gas in an amount of 0.2 to 2.0 times the volume of the yeast, extruding the resultant mixture at a pressure of from 1 to 10 atmospheres through orifices having a length to diameter ratio of about 1:1 to 4:1 and allowing escape of gas from the interior of the resultant extrudate to form pores communicating with the surface to produce porous granules of active yeast. The granules may be dried to produce active dry yeast granules having a total surface area formed by the outside surface plus the interior surface of the granules of at least 1.5 times the total area of the outside surface of the granules. The active dry yeast granules have good vitality and are capable of rapid rehydration and regeneration.
    Type: Grant
    Filed: January 19, 1981
    Date of Patent: June 15, 1982
    Assignee: Deutsche Hefewerke GmbH
    Inventors: Franz-Josef Carduck, Dietrich Kloetzer, Gerard Veldman
  • Patent number: 4328250
    Abstract: A dry yeast composition in particulate form containing at least 92% dry matter is prepared consisting essentially of active dry bakers' yeast capable of fermenting sweetened doughs containing more than 5% sugar and an emulsifying agent having an HLB value of between 3 and 11. The emulsifying agent is added to the yeast before drying and protects the yeast during drying.
    Type: Grant
    Filed: August 2, 1978
    Date of Patent: May 4, 1982
    Assignee: Societe Industrielle Lesaffre
    Inventors: Philippe Clement, Jean-Paul Rossi
  • Patent number: 4262023
    Abstract: The invention relates to cheese starter compositions comprising a plurality of discrete pellets containing frozen, live bacteria, wherein each pellet contains at least one strain of bacterium which is not appreciably present in at least one other of the pellets. In a preferred form of the invention, each pellet contains a single strain of bacterium, while in the composition as a whole there are at least two and preferably more strains.
    Type: Grant
    Filed: December 18, 1978
    Date of Patent: April 14, 1981
    Assignee: Mauri Brothers & Thomson (Aust.) Pty. Limited
    Inventors: Denis I. Eddy, John P. Grace
  • Patent number: 4258070
    Abstract: A method for inhibiting microbial contamination, particularly applicable to processes for preparing food materials under nominally aseptic conditions, is carried out by cycling the temperature of a desired portion of the process which is a source of contamination between upper and lower limits which fall between 0.degree. and 60.degree. C. and which are at least 5.degree. C. apart at a frequency of about 0.2 to 5 cycles per hour to create a varying thermal environment at the source of contamination which slows microbial growth.
    Type: Grant
    Filed: October 4, 1978
    Date of Patent: March 24, 1981
    Assignee: Standard Oil Company (Indiana)
    Inventors: John A. Ridgway, Jr., William P. Weisrock
  • Patent number: 4250264
    Abstract: A medium for growing bacteria from an initial population to a final predetermined population where growth of the bacteria substantially subsides due to the lack of nutrient.
    Type: Grant
    Filed: May 21, 1979
    Date of Patent: February 10, 1981
    Assignee: Minnesota Mining and Manufacturing Company
    Inventors: Robert L. Nelson, James F. Drake
  • Patent number: 4246349
    Abstract: Bacteria immobilized by adsorption on an inorganic carrier are stabilized by carrying out the adsorption procedure in the presence of from about 1 to about 20% weight per volume of sucrose of nonfat dry milk solids and lyophilizing the adsorbed bacteria.
    Type: Grant
    Filed: September 5, 1978
    Date of Patent: January 20, 1981
    Assignee: Corning Glass Works
    Inventors: Ralph A. Messing, Robert A. Oppermann, William S. Ramsey, Milton M. Takeguchi
  • Patent number: 4245039
    Abstract: A homogeneous suspension, in a buffered aqueous solution having a pH of 7.0 to 7.7, of a destroyed microorganism which is positive to the clumping factor, said suspension containing from 3 to 50 percent by weight of at least one polyhydric alcohol soluble therein, is disclosed, as are its manufacture and its use as a reagent for the determination of fibrinogen and for fibrin cleavage products.
    Type: Grant
    Filed: June 8, 1976
    Date of Patent: January 13, 1981
    Assignee: Behringwerke AG
    Inventors: Norbert Heimburger, Friedrich Brauns, Kurt Fischer
  • Patent number: 4229544
    Abstract: A method of packaging, and the package so produced, for maintaining living organisms viable for a long period of time. The living organisms, such as bacteria, fungi, algae, etc., are mixed with a carrier, such as peat. The organism-carrier mixture is then disposed in a package, such as a heat-sealable plastic envelope, and a gaseous atmosphere is provided in the package effective to induce and maintain substantial nonvegatative state formation of the organisms. Some organisms will form cysts, others spores, but whatever nonvegetative state is assumed, the organisms will be much less susceptible to heat, cold, starvation, and other adverse environmental factors. Suitable gaseous atmospheres include nitrogen, helium, and argon gases. The package is then sealed to prevent contamination of the atmosphere therein.
    Type: Grant
    Filed: August 8, 1978
    Date of Patent: October 21, 1980
    Assignee: Payfer Laboratories Inc.
    Inventors: Robert W. Haynes, William H. Rasmussen
  • Patent number: 4226940
    Abstract: A concentrated bacterial culture, capable of being cooled to temperatures as low as about -40.degree. C. for storage without rapid freezing and with minimum damage to the bacterial cells, is prepared by diluting a conventionally prepared concentrated cell paste with a liquid anti-freeze agent containing one or more water freezing point depressants which are water-soluble, are non-injurious to the bacteria, and do not form crystals when cooled to a predetermined temperature within the range of about 5 to about -40.degree. C. The amount of the freezing point depressant(s) is sufficient to prevent formation of ice crystals from the water present in the diluted product when cooled to the predetermined temperature. The culture, which does not become hard or crystalline upon being cooled to temperatures as low as -40.degree. C., can be warmed to a temperature convenient for sampling, assaying and blending and then re-cooled to a cold storage temperature without an appreciable reduction in viability.
    Type: Grant
    Filed: June 21, 1979
    Date of Patent: October 7, 1980
    Assignee: Great Lakes Biochemical Co., Inc.
    Inventor: Arnold B. Storrs
  • Patent number: 4217419
    Abstract: A dried lactic acid bacteria composition comprising lactic acid bacteria and alginate, which is effective for the preservation of said bacteria therein.
    Type: Grant
    Filed: October 27, 1977
    Date of Patent: August 12, 1980
    Assignee: Ajinomoto Company, Incorporated
    Inventor: Tadao Suzuki
  • Patent number: 4217420
    Abstract: An active dried bakers' yeast is prepared with a dry matter content of at least 85% by weight, a protein content (%N.times.6.25) of 45 to 60% based on dry matter and an activity value of 420 to 600, when determined according to test method B.sup.2 as defined in the specification. The yeast has the advantages that it can be added as such to the flour prior to dough-making without preliminary soaking, and it is capable of better gas production than active dried yeasts hitherto available.
    Type: Grant
    Filed: September 18, 1978
    Date of Patent: August 12, 1980
    Assignee: Gist-Brocades N.V.
    Inventor: Arend Langejan
  • Patent number: 4205132
    Abstract: Storage stable, lyophilized, acid producing bacteria, such as lactic acid bacteria, are prepared by lyophilizing the bacteria in the presence of at least 5% by weight of the bacteria of a basic organic or inorganic buffering agent, preferably an alkali metal salt of glycerophosphate, to provide lyophilized bacteria containing less than 5% by weight water, and sealing the lyophilized bacteria in a container while in a vacuum or surrounded by a gaseous atmosphere substantially free of oxygen, preferably argon which is essentially free of oxygen.
    Type: Grant
    Filed: July 17, 1978
    Date of Patent: May 27, 1980
    Assignee: Microlife Technics, Inc.
    Inventors: William E. Sandine, Ebenezer R. Vedamuthu
  • Patent number: 4172127
    Abstract: In a feedlot operation wherein ruminant animals, such as cattle or sheep, are fed ad libitum a high-energy ration or feed, lactic acidosis is greatly reduced or eliminated and weight gain and feed conversion are increased by administering to the animal the microorganism Peptococcus asaccharolyticus upon introduction of the animal to the feedlot. The microorganism is conveniently administered to the animal, either by direct injection or introduction into the rumen via a needle or stomach tube or in admixture with the feed or ration. The microorganism is also useful in the treatment of cattle (calves) and sheep ill with lactic acidosis.
    Type: Grant
    Filed: June 22, 1978
    Date of Patent: October 23, 1979
    Assignee: Research Corporation
    Inventor: Thomas L. Huber
  • Patent number: 4161397
    Abstract: A liquid seed treatment composition is described in which microdried bacteria are flowably suspended in a nonphytotoxic liquid carrier with a chemical substance normally toxic to the bacteria. The bacteria in the compositions exhibit prolonged viability relative to dry, non-fluid formulations. To sustain bacterial viability and effectiveness of the compositions, the compositions are maintained as a liquid colloid in which the ingredients are thoroughly dispersed.
    Type: Grant
    Filed: August 8, 1977
    Date of Patent: July 17, 1979
    Assignee: Kalo Laboratories, Inc.
    Inventors: Eugene M. Bellet, Madan M. Joshi