Separation Of Micro-organism From Culture Media Patents (Class 435/261)
  • Patent number: 9632085
    Abstract: Embodiments of various aspects described herein are directed to methods, compositions, kits and systems for rapid determination of antibiotic susceptibility of a microbe within hours after a sample is collected. In some embodiments, the methods, compositions, kits and systems described herein can allow determination of antibiotic susceptibility of a microbe based on a small number of microbes, e.g., as few as 5-10 microbes bound to a microbe-targeting substrate described herein.
    Type: Grant
    Filed: February 28, 2013
    Date of Patent: April 25, 2017
    Assignee: President and Fellows of Harvard College
    Inventors: Michael Super, Donald E. Ingber, Mark J. Cartwright, Alexander Watters, John Samuel Workman, Daniel Levner, Martin Marcus Rottman
  • Publication number: 20150147811
    Abstract: The invention relates to a method for separating off a liquid supernatant from cells, comprising the steps: providing a mixture of the cells with a liquid, charging a first filter housing with the mixture, wherein in the filter housing a filter having a pore size of between 4 ?m and 50 ?m is provided on a flat base surface pierced in a sieve manner and the walls of the filter housing are connected so as to seal with the flat base surface that is pierced in a sieve manner, applying a differential pressure of at least 0.5 bar on the mixture, as a result of which the liquid portion of the mixture is forced through the filter and a filter cake containing cells remains in the filter housing, and removal of the filtered liquid.
    Type: Application
    Filed: July 19, 2013
    Publication date: May 28, 2015
    Inventors: Grosse Thomas, Niederkrüger Holger, Schaaf Andreas
  • Patent number: 9012201
    Abstract: A biological component enrichment unit for the isolation, purification and/or determination of a biological component using particles (3) and/or another solid phase, which enrichment unit comprises at least one sample container (1, 15, 26, 32, 39) for the realization of the biological sample method. The enrichment unit comprises a lead-through structure (5), such as a bushing (21, 27) made up of one or several parts or a cover having one or several openings (25, 36). The lead-through structure and one or several sample containers can be brought into connection with one another so that, when the lead-through structure is placed on top of the sample container, a functional unit is formed for the realization of the biological method, such as for the binding of the component, the growing, isolation, purification, enrichment of the bacteria or cell or for another similar method.
    Type: Grant
    Filed: December 4, 2006
    Date of Patent: April 21, 2015
    Assignee: BioControl Systems, Inc.
    Inventors: Matti Korpela, Tytti Miettinen, Tuija Tenhunen, Teemu Korpimäki, Pekka Mattsson, Mika Tuomola
  • Patent number: 8986945
    Abstract: Provided are methods and compositions for isolating and detecting rare cells from a biological sample containing other types of cells, particularly including debulking that uses a microfabricated filter for filtering samples. The enriched rare cells can be used in a downstream process such as identification, characterization or growth in culture, or in other ways. Also included is a method of determining tumor aggressiveness or the number or proportion of cancer cells in the enriched sample by detecting telomerase activity, nucleic acid or expression after enrichment of rare cells. Also provided is an efficient, rapid method to specifically remove red and white blood cells from a biological sample containing at least one of the cell types, leading to enrichment of rare target cells including circulating tumor (CTC), stromal, mesenchymal, endothelial, fetal, stem, or non-hematopoietic cells et cetera from a blood sample.
    Type: Grant
    Filed: August 20, 2007
    Date of Patent: March 24, 2015
    Assignee: Aviva Biosciences Corporation
    Inventors: Ping Lin, Andrea Ghetti, Wenge Shi, Mengjia Tang, Gioulnar I. Harvie, Huimin Tao, Guoliang Tao, Lei Wu, David Cerny, Jia Xu, Douglas T. Yamanishi
  • Patent number: 8986944
    Abstract: The present invention includes methods of enriching rare cells, such as cancer cells, from biological samples, such as blood samples. The methods include performing at least one debulking step on a blood sample and selectively removing at least one type undesirable component from the blood sample to obtain a blood sample that is enriched in a rare cell of interest. In some embodiments magnetic beads coupled to specific binding members are used to selectively removed components.
    Type: Grant
    Filed: August 2, 2006
    Date of Patent: March 24, 2015
    Assignee: Aviva Biosciences Corporation
    Inventors: Douglas T. Yamanishi, Paul G. Hujsak, Sara F. Snyder, George Walker, Junquan Xu, Mingxian Huang, Guoliang Tao, Lei Wu, Xiaobo Wang, Joe Ouyang, Charina Schmitigal, Jing Cheng, Jia Xu
  • Publication number: 20150079582
    Abstract: A bismuth-containing concentration agent for microorganisms is provided. Additionally, articles that include the concentration agent and methods of concentrating a microorganism using the concentration agent are provided.
    Type: Application
    Filed: March 4, 2013
    Publication date: March 19, 2015
    Inventors: Manjiri T. Kshirsagar, Evan Koon Lun Yuuji Hajime, Andrew W. Rabins
  • Patent number: 8968687
    Abstract: A method for recycling metals is provided by using extracellular proteins excreted by a specific thermophilic bacteria strain, Tepidimonas fonticaldi sp. nov., in which the extracellular proteins show excellent metal-ion binding ability, being useful in recycling rare earth metal ions and precious metal ions from geothermal fluids, boiler solutions, industrial wastewater or hard water.
    Type: Grant
    Filed: December 30, 2013
    Date of Patent: March 3, 2015
    Assignee: Industrial Technology Research Institute
    Inventors: Yin-Lung Han, Tai-Rong Guo, Jo-Shu Chang, Yung-Chong Lou, Wan-Ju Yu
  • Patent number: 8969066
    Abstract: The present invention provides compositions and methods for producing flocculation moieties in photosynthetic organisms. The photosynthetic organisms are genetically modified to effect production, secretion, or both, of the flocculation moieties. Also provided are methods of flocculating organisms.
    Type: Grant
    Filed: June 26, 2009
    Date of Patent: March 3, 2015
    Assignee: Sapphire Energy, Inc.
    Inventors: Michael Mendez, Craig Behnke, Yan Poon, Philip Lee
  • Patent number: 8962235
    Abstract: A method for sorting particles, in particular cells A and B. The method uses a single channel with only one input and only one output. A particle mix A and B in a fluid is introduced into the channel and particles within the channel are sorted.
    Type: Grant
    Filed: September 20, 2007
    Date of Patent: February 24, 2015
    Assignee: The University Court of the University of St. Andrews
    Inventors: Michael MacDonald, Kishan Dholakia, Igor Andreev
  • Patent number: 8951765
    Abstract: Embodiments described herein relate to separating and/or concentrating target cells from a carrier fluid that may include other non-target cells. Embodiments include a cell separator with a flow surface having indentations formed thereon. The indentations are configured to capture target cells by physical and/or chemical interactions. The indentations may also include a layer of support molecules that assist in releasing captured cells for collection.
    Type: Grant
    Filed: November 24, 2009
    Date of Patent: February 10, 2015
    Assignee: Empire Technology Development LLC
    Inventor: Masahiro Ueda
  • Patent number: 8900832
    Abstract: The present invention relates to a method for the production of fat with a principal application as transportation biofuel or a component or raw material therefor. According to the method, cell masses, cell suspensions and/or liquid phases formed in the production of single cell oil, and/or biomass-containing side streams or microorganism cell masses for another purpose and/or originating from other sources, are contacted with a fat-production capable microorganism and the organism is allowed to produce fat. The resulting fat is recovered or the microorganism mass is passed to a single-cell oil production process. By means of the invention, the organic matter present in the cell mass and side streams of single-cell oil can be re-utilized for the production of the single-cell oil, thereby improving a total fat yield, as well as reducing an organic load of the side streams.
    Type: Grant
    Filed: June 24, 2010
    Date of Patent: December 2, 2014
    Assignee: Neste Oil Oyj
    Inventors: Heidi Kahelin, Simo Laakso, Ossi Pastinen, Miia Mujunen, Tarja Suomalainen
  • Patent number: 8889388
    Abstract: The invention provides a settling device comprising an acoustic wave generator and an inclined settling chamber. The angle ? between the acoustic wave direction and the inclined settling chamber is greater than 0 and less than 90°. The invention also provides a concentration method and a separation method using the device. The invention can be used to concentrate or separate particles such as inorganic particles, organic particles, and biological particles, for example, mammalian cells, bacteria, yeast, algae, and plant cells. The invention exhibits technical merits such as higher efficiency, cost-effectiveness, and large-scale production.
    Type: Grant
    Filed: April 21, 2011
    Date of Patent: November 18, 2014
    Inventors: Zhaowei Wang, Donald Feke, Joanne Belovich
  • Publication number: 20140335600
    Abstract: A method for collecting cells that have been cultured by using a cell culture kit in which, at least, a culture container for cultivating cells, a cell collection container for collecting cells after cultivation and a waste liquid container for collecting a culture medium after cultivation are linked to one another through a conduit, the method including transferring a culture supernatant that has been cultured from the culture container to the waste liquid container, transferring a concentrated suspension of cells from the culture container to the cell collection container, returning part of the culture supernatant discharged in the waste liquid container to the culture container and allowing cells remaining in the culture container to be suspended in a suspension of cells, and transferring again a suspension of cells in which remaining cells are suspended from the culture container to the cell collection container.
    Type: Application
    Filed: July 25, 2014
    Publication date: November 13, 2014
    Inventors: Satoshi Tanaka, Ryo Suenaga, Masahiro Kuninori, Kyohei Ota
  • Patent number: 8841104
    Abstract: The invention generally relates to methods of using compositions that include sets of magnetic particles, members of each set being conjugated to an antibody specific for a pathogen, and magnets to isolate a pathogen from a body fluid sample.
    Type: Grant
    Filed: April 21, 2011
    Date of Patent: September 23, 2014
    Assignee: NanoMR, Inc.
    Inventors: Sergey A. Dryga, Victor C. Esch, Lisa-Jo Ann Clarizia, Eddie W. Adams, Thearith H. Ung, Ravil A. Sitdikov
  • Publication number: 20140273176
    Abstract: Systems and methods for processing biological material utilizing chitosan are provided herein. Methods may include the steps of reducing the pH of an amount of culture to about 5 to 7, incorporating an amount of a chitosan solution into the culture to form a mixture, wherein chitosan in the chitosan solution binds to the biological material; increasing the pH of the culture to about 7 to 10, to flocculate biological material in the culture, concentrating the flocculates, reducing the pH of the concentrated flocculates to about 3 to 5, and separating the recovered chitosan solution from the concentrated biological material.
    Type: Application
    Filed: March 15, 2013
    Publication date: September 18, 2014
    Inventor: Daniel Fleischer
  • Patent number: 8828238
    Abstract: A novel thermophilic bacterium is provided, which has a 16S rDNA sequence as set forth in SEQ ID NO. 1. The thermophilic bacterium excretes extracellular proteins having excellent metal-ion binding ability, being useful in the treatment of boiler equipment, pipelines, geothermal wells or industrial wastewater or hard water.
    Type: Grant
    Filed: March 18, 2013
    Date of Patent: September 9, 2014
    Assignee: Industrial Technology Research Instiute
    Inventors: Yin-Lung Han, Tai-Rong Guo, Jo-Shu Chang, I-Ju Chou
  • Patent number: 8772020
    Abstract: A fermentation system for production of biogas from solid organic material, the system including at least one fermentation reactor. The fermentation reactor includes a vessel (37) for housing a feed mixture having liquid and solid particles and an outlet port (44), a strainer assembly (33) at the outlet port for filtering processed feed mixture from the vessel, and a biogasification reactor (37) in fluid communication with another fermentation reactor, the other fermentation reactor configured to produce a biomethane. The strainer assembly further includes a mixture driving member (53) positioned in the strainer body (47) adjacent the drain opening (51), the mixture driving member having a driving surface configured to receive the liquid passing through the filter member (60). The driving surface engages the filter member such that solid particles are driven from a surface of the filter member when the driving member is activated.
    Type: Grant
    Filed: August 1, 2007
    Date of Patent: July 8, 2014
    Assignee: The Regents of the University of California
    Inventors: Dave Konwinski, Ruihong Zhang
  • Patent number: 8772004
    Abstract: A method and device for aggregating algae in an aqueous solution is disclosed. The method can include providing an algae feed comprising a liquid and algae dispersed therein. The algae feed can be aggregated by applying a nanosecond pulsed electric field to the algae feed. The nanosecond pulsed electric field can include a plurality of electric pulses having a pulse duration ranging from 1 to 1,000 nanoseconds. The method can also include separating an aggregated algae stream from the algae feed and feeding the aggregated algae stream to a lipid extraction operation.
    Type: Grant
    Filed: June 25, 2010
    Date of Patent: July 8, 2014
    Assignee: Old Dominion University Research Foundation
    Inventors: Gary C. Schafran, Juergen F. Kolb, Aron Stubbins, Karl H. Schoenbach
  • Patent number: 8741629
    Abstract: Methods for selective extraction and fractionation of algal proteins from an algal biomass or algal culture are disclosed. A method of selective removal of products from an algal biomass provides for single and multistep extraction processes which allow for efficient separation of algal proteins. These proteins can be used as renewable sources of proteins for animal feedstocks and human food. Further, lipids remaining in the algal biomass after extraction of proteins can be used to generate renewable fuels.
    Type: Grant
    Filed: November 4, 2011
    Date of Patent: June 3, 2014
    Assignee: Heliae Development, LLC
    Inventor: Aniket Kale
  • Patent number: 8734649
    Abstract: A method of dewatering algae and recycling water therefrom is presented. A method of dewatering a wet algal cell culture includes removing liquid from an algal cell culture to obtain a wet algal biomass having a lower liquid content than the algal cell culture. At least a portion of the liquid removed from the algal cell culture is recycled for use in a different algal cell culture. The method includes adding a water miscible solvent set to the wet algal biomass and waiting an amount of time to permit algal cells of the algal biomass to gather and isolating at least a portion of the gathered algal cells from at least a portion of the solvent set and liquid of the wet algal biomass so that a dewatered algal biomass is generated. The dewatered algal biomass can be used to generated algal products such as biofuels and nutraceuticals.
    Type: Grant
    Filed: April 6, 2011
    Date of Patent: May 27, 2014
    Assignee: Heliae Development, LLC
    Inventor: Aniket Kale
  • Patent number: 8703474
    Abstract: Disclosed are a novel flavor compound-producing yeast strain and methods of using the strain to produce flavor compounds.
    Type: Grant
    Filed: August 16, 2011
    Date of Patent: April 22, 2014
    Assignee: Academia Sinica
    Inventors: Jui-Jen Chang, Cheng-Yu Ho, Chieh-Chen Huang, Ming-Che Shih, Wen-Hsiung Li
  • Publication number: 20140099700
    Abstract: A bioreactor includes a support structure and a translucent liner. A containing system of bioreactors includes a plurality of bioreactors. A contained bioreactor system includes a plurality of containing systems of interconnected bioreactors, an industrial blower, an air filter, a microbial filter, a gas delivery system, and a harvesting station. A method of reducing carbon dioxide emissions includes delivering carbon dioxide to a bioreactor where the bioreactor includes a support structure and a liner where the bioreactor includes media containing microbes. A method of harvesting microorganisms includes emptying the solution and removing a sieve from the harvesting unit.
    Type: Application
    Filed: October 4, 2012
    Publication date: April 10, 2014
    Applicant: ENERGAIA, INC.
    Inventors: Saumil Navnit Shah, Derek Andrew Blitz, Patsakorn Thaveeuchukorn
  • Patent number: 8685734
    Abstract: The present invention is directed to an improved method of purifying virus, particularly reovirus. Infectious virus can be extracted from a cell culture with a detergent to produce high titers of virus, and the virus can then be purified by simple steps such as filtration and column chromatography. Viruses and compositions comprising the viruses prepared according to the present invention are also provided.
    Type: Grant
    Filed: April 17, 2007
    Date of Patent: April 1, 2014
    Assignee: Oncolytics Biotech Inc.
    Inventor: Matthew C. Coffey
  • Publication number: 20140073034
    Abstract: Highly efficient and rapid filtration-based concentration devices, systems and methods are disclosed with sample fluidic lines and a filter packaged in a disposable tip which concentrate biological particles that are suspended in liquid from a dilute feed suspension. A sample concentrate or retentate suspension is retained while eliminating the separated fluid in a separate flow stream. The concentrate is then dispensed from the disposable tip in a set volume of elution fluid. Suspended biological particles include such materials as proteins/toxins, viruses, DNA, and/or bacteria in the size range of approximately 0.001 micron to 20 microns diameter. Concentration of these particles is advantageous for detection of target particles in a dilute suspension, because concentrating them into a small volume makes them easier to detect. All conduits by which the disposable tip attaches to the instrument are combined into a single connection point on the upper end of the tip.
    Type: Application
    Filed: November 19, 2013
    Publication date: March 13, 2014
    Applicant: INNOVAPREP LLC
    Inventors: Andrew Edward Page, Zachary A. Packingham, David Scott Alburty, Alec D. Adolphson
  • Publication number: 20140004605
    Abstract: The present invention provides methods for the isolation of oils from intact or lysed microorganisms in aqueous media with pressurized carbon dioxide as a solute. Such oils may be used for the production of biofuels.
    Type: Application
    Filed: August 16, 2011
    Publication date: January 2, 2014
    Applicants: SYNAPTIC RESEARCH LLC, THE JOHNS HOPKINS UNIVERSITY
    Inventors: Marc D. Donohue, Michael J. Betenbaugh, George A. Oyler, Julian N. Rosenberg
  • Patent number: 8614083
    Abstract: A method for the simultaneous concentration of multiple toxins from large volumes of water. The method includes the steps of providing a disposable separation centrifuge bowl, the centrifuge bowl including a positively charged material at it's inner core. A large water sample contaminated with toxins from a group consisting of protozoa, bacteria, bacterial spores, and toxins is delivered to the centrifuge bowl. A centrifugal force is applied to the separation bowl. The water sample is concentrated to remove large particles of the toxins in the bowl due to the centrifugal forces. The concentrated water sample is passes through the positively charged inner core to capture any remaining concentrated targets by electrostatic forces and the concentrated targets are eluted.
    Type: Grant
    Filed: June 13, 2008
    Date of Patent: December 24, 2013
    Assignees: Trustees of Tufts College, Haemonetics
    Inventors: Saul Tzipori, Udi Zukerman, Gary Stacey
  • Publication number: 20130337546
    Abstract: A process for concentrating microorganism-containing suspensions comprising (a) centrifuging a microorganism-containing suspension to provide a first microorganism-containing concentrate and a supernatant liquid; (b) filtering said first microorganism-containing concentrate, to provide a permeate and a second microorganism-containing concentrate.
    Type: Application
    Filed: August 21, 2013
    Publication date: December 19, 2013
    Applicant: Danisco A/S
    Inventors: Lars Wexoe Petersen, Eric Hohol, Steve E. von Dollen
  • Patent number: 8586338
    Abstract: Ligand functionalized substrates, methods of making ligand functionalized substrates, and methods of using functionalized substrates are disclosed.
    Type: Grant
    Filed: May 27, 2009
    Date of Patent: November 19, 2013
    Assignees: 3M Innovative Properties Company, Wisconsin Alumni Research Foundation
    Inventors: Mark R. Etzel, Yi He, Steven M. Heilmann, Jerald K. Rasmussen, Kannan Seshadri, Simon K. Shannon, Clinton P. Waller, Jr., Douglas E. Weiss
  • Patent number: 8574887
    Abstract: A method to grow and concentrate algae by adding a bacterium to an aqueous algal solution. After the bacteria are added, the algae and bacterial precipitate out of solution and produce a viable algal concentration that is 30 to 45 percent algae by wet weight.
    Type: Grant
    Filed: November 19, 2010
    Date of Patent: November 5, 2013
    Assignee: Core Intellectual Properties Holdings, LLC
    Inventors: Gary Scott Stepenoff, Karin L. Hastings
  • Patent number: 8569009
    Abstract: Provided are methods and compositions for isolating and detecting rare cells from a biological sample containing other types of cells, particularly including debulking that uses a microfabricated filter for filtering samples. The enriched rare cells can be used in a downstream process such as identification, characterization or growth in culture, or in other ways. Also included is a method of determining tumor aggressiveness or the number or proportion of cancer cells in the enriched sample by detecting telomerase activity, nucleic acid or expression after enrichment of rare cells. Also provided is an efficient, rapid method to specifically remove red and white blood cells from a biological sample containing at least one of the cell types, leading to enrichment of rare target cells including circulating tumor (CTC), stromal, mesenchymal, endothelial, fetal, stem, or non-hematopoietic cells et cetera from a blood sample.
    Type: Grant
    Filed: August 20, 2007
    Date of Patent: October 29, 2013
    Assignee: Aviva Biosciences Corporation
    Inventors: Ping Lin, Andrea Ghetti, Wenge Shi, Mengjia Tang, Gioulnar I. Harvie, Huimin Tao, Guoliang Tao, Lei Wu, David Cerny, Jia Xu
  • Patent number: 8557564
    Abstract: Provided is a method of separating microorganisms from a sample including contacting the sample containing microorganisms with an inorganic on exchange material such that the sample reacts with the inorganic on exchange material, and contacting the reacted sample with a means for capturing microorganisms.
    Type: Grant
    Filed: December 14, 2011
    Date of Patent: October 15, 2013
    Assignee: Samsung Electronics Co., Ltd.
    Inventors: Kyu-youn Hwang, Sung-young Jeong, Joon-ho Kim
  • Patent number: 8557539
    Abstract: A new device and method for detecting the presence of living microorganisms in test samples are described. The device comprises a container with at least one section transparent to light, a growth zone located in said container containing a mixture of growth media capable of supporting growth of the microorganisms, and at least one indicator substrate that changes its optical properties due to growth of the microorganisms. A detection zone is located in the container adjacent to the transparent section, and a barrier layer comprising porous solid material separates the two zones, allowing diffusion of molecules and ions of metabolic by-products of the organisms, while preventing microorganisms and particulate matter of the test sample from penetrating into the detection zone.
    Type: Grant
    Filed: June 21, 2007
    Date of Patent: October 15, 2013
    Assignee: BioLumix Inc.
    Inventors: Gideon Eden, Ruth Eden
  • Patent number: 8541226
    Abstract: This invention relates to a bioreactor for producing high rates of hydrogen from plant biomass. It also relates to the rapid screening, selection and isolation of biofilm forming mesophilic and/or thermophilic bacteria or bacteria consortia that generate high levels of hydrogen from plant biomass or from soluble hydrolysates derived from the hydrolysis of cellulosic materials including hemicellulose. The reactor comprises a primary reactor vessel having a bed of hydrogen producing bacteria towards its base located within a secondary reactor vessel which functions as a hydrogen gas collector and as a clarifier and separator. The plant biomass may be one or a mixture of insoluble cellulosic material and a hydrolysate derived from hydrolysis of cellulosic material. In one embodiment the bed of the primary reactor vessel is fluidised by recycling hydrogen gas saturated plant biomass effluent from the secondary reactor vessel to the primary reactor vessel.
    Type: Grant
    Filed: September 9, 2008
    Date of Patent: September 24, 2013
    Assignee: University of the Witwatersrand, Johannesburg
    Inventor: Vincent Myles Gray
  • Patent number: 8512998
    Abstract: Conditioning and concentration of microalgae are accomplished by the process steps of grinding a dilute aqueous dispersion of microalgae in the presence of grinding media and then applying adsorptive bubble separation. This process is amenable to the use of dilute feed microalgal dispersions such as are encountered in the production of algal biomass for biofuel applications.
    Type: Grant
    Filed: June 18, 2008
    Date of Patent: August 20, 2013
    Assignees: Renewable Algal Energy, LLC, Eastman Chemical Company
    Inventors: Robert L. Clayton, Stephen N. Falling, Jeffrey S. Kanel
  • Patent number: 8481300
    Abstract: Provided are a method for removing and/or absorbing greenhouse gases using aquatic plant and/or algae having a greenhouse gas absorbing performance and resistance to toxic substances, a method for producing bioenergy using predators with high fatty acids, and a method for producing bioenergy including cultivating aquatic plant and/or algae having a greenhouse gas absorbing performance and resistance to toxic substances, while supplying a waste gas mixture to the aquatic plant and/or algae, (S2) cultivating predators having a capability to prey on the aquatic plant and/or algae while supplying the aquatic plant and/or algae to the predators, (S3) harvesting the predators, and (S4) converting the harvested predators into bioenergy.
    Type: Grant
    Filed: February 15, 2011
    Date of Patent: July 9, 2013
    Assignee: SNU R&DB Foundation
    Inventors: Hae-Jin Jeong, Jae-Yeon Park, Yoeng-Do Yoo, Nam-Seon Kang, Jung-Rhe Rho
  • Patent number: 8470585
    Abstract: The invention provides methods for large-scale adenovirus purification from high cell density suspensions, using host cell DNA fragmentation and/or precipitation followed by a clarification step with tangential flow filtration.
    Type: Grant
    Filed: October 14, 2010
    Date of Patent: June 25, 2013
    Assignee: Crucell Holland B.V.
    Inventors: Marcel Leo De Vocht, Marloes Veenstra
  • Publication number: 20130157355
    Abstract: Biocontainers, as well as methods and platforms for rocking the biocontainers, are disclosed.
    Type: Application
    Filed: December 20, 2011
    Publication date: June 20, 2013
    Applicant: Pall Corporation
    Inventors: Timothy Alan Barrett, Alison Jane West, Kenneth Roy Weight, Adam Peter Westwood, Daniel James Kesselaar
  • Patent number: 8460920
    Abstract: The invention provides methods for large-scale adenovirus purification from high cell density suspensions, using host cell DNA precipitation followed by a clarification step.
    Type: Grant
    Filed: October 14, 2010
    Date of Patent: June 11, 2013
    Assignee: Crucell Holland B.V.
    Inventors: Marcel Leo De Vocht, Marloes Veenstra
  • Patent number: 8449877
    Abstract: The present invention provides a method for purifying bacterial minicells that involves subjecting a sample containing minicells to density gradient centrifugation in a biologically compatible medium. The method optionally includes a preliminary differential centrifugation step and one or more filtration steps. The invention also provides a method for purifying bacterial minicells in which a sample containing minicells is subjected to a condition that induces parent bacterial cells to adopt a filamentous form, followed by filtration of the sample to separate minicells from parent bacterial cells. The inventive methods optionally include one or more steps to remove endotoxin from purified minicell preparations, and/or treatment of purified minicell preparations with an antibiotic. Additionally, the invention provides purified minicell preparations, prepared according to the foregoing methods, and containing fewer than about 1 contaminating parent bacterial cell per 107, 108, 109, 1010, or 1011 minicells.
    Type: Grant
    Filed: April 4, 2011
    Date of Patent: May 28, 2013
    Assignee: Engeneic Molecular Delivery Pty. Ltd.
    Inventors: Himanshu Brahmbhatt, Jennifer MacDiarmid
  • Publication number: 20130109079
    Abstract: A method for separating an organic component from a mixture containing the organic component, water, and a microorganism, the method comprising (a) subjecting the mixture to a first separation step under mild conditions in order to generate a first stream containing water and organic component, and a second stream containing water and microorganism; and (b) subjecting the first stream to a second separation step under conditions which result in separation of the organic component from the water. The method allows for the organic component to be separated from the water under relatively severe conditions, due to the prior removal of the microorganism, and thus makes it possible to recycle the undamaged microorganism. The organic component may comprise a microbial oil which is suitable for use as, or conversion to, a biofuel.
    Type: Application
    Filed: October 31, 2012
    Publication date: May 2, 2013
    Applicant: SHELL OIL COMPANY
    Inventor: Shell Oil Company
  • Publication number: 20130089917
    Abstract: A method and apparatus for cell harvest of production scale quantities of cell cultures using single use components comprising a flexible membrane mounted on a rigid frame and is supported within a multiple use rigid centrifuge bowl, such single use components including a core with an increased diameter and an internal truncated cone shape in order to permit the system to maintain a sufficiently high angular velocity to create a settling velocity suited to efficiently processing highly concentrated cell culture streams. Features which minimize feed turbidity, and others which permit the continuous or semi-continuous discharge of cell concentrate, increase the overall production rate over the rate which can be achieved using current intermittent processing methods for large cell culture volumes. Injection of a diluent during the cell concentrate removal process permits more complete removal of viscous cell concentrates.
    Type: Application
    Filed: November 21, 2012
    Publication date: April 11, 2013
    Applicant: PNEUMATIC SCALE CORPORATION
    Inventor: Pneumatic Scale Corporation
  • Patent number: 8377672
    Abstract: Ligand functionalized substrates, methods of making ligand functionalized substrates, and methods of using functionalized substrates are disclosed.
    Type: Grant
    Filed: January 7, 2011
    Date of Patent: February 19, 2013
    Assignee: 3M Innovative Properties Company
    Inventors: Jerald K. Rasmussen, Catherine A. Bothof, Kannan Seshadri, Erin A. Satterwhite, Robert T. Fitzsimons, James I. Hembre, Mahfuza B. Ali
  • Patent number: 8357526
    Abstract: The present disclosure relates to isolation and identification of unique Pseudomonas stutzeri strains that can grow on crude oil under denitrifying conditions and are useful in oil recovery.
    Type: Grant
    Filed: April 18, 2008
    Date of Patent: January 22, 2013
    Assignee: E.I. du Pont de Nemours and Company
    Inventors: Sharon Jo Keeler, Robert D. Fallon, Edwin R. Hendrickson, Linda L. Hnatow, Scott Christopher Jackson, Michael P. Perry
  • Patent number: 8343483
    Abstract: New strains of Lactobacillus that have been selected for their capability of improved reduction the number of Streptococcus mutans in the mouth of mammals through inhibiting activity in combination with better binding to the oral mucins and dental plaque, thereby preventing, reducing or treating dental caries, and products derived from said strains, including agents for treatment or prophylaxis of caries for administration to humans.
    Type: Grant
    Filed: October 15, 2008
    Date of Patent: January 1, 2013
    Assignee: Biogaia AB
    Inventors: Bo Mollstam, Eamonn Connolly
  • Publication number: 20120238003
    Abstract: A microorganism-concentrating apparatus according to one embodiment includes a mixing device, a filtering device, and a magnetic separation device. The mixing device is configured to mix an aqueous suspension of microorganisms with a particulate filter aid includes magnetic particles to prepare an aqueous slurry of the microorganisms and the particulate filter aid. The filtering device is configured to filter the aqueous slurry supplied from the mixing device to provide a filter cake includes the microorganisms and the particulate filter aid, and to provide a filtrate. The magnetic separation device is configured to magnetically separate the filter cake supplied from the filtering device into the microorganisms and the particulate filter aid.
    Type: Application
    Filed: August 3, 2011
    Publication date: September 20, 2012
    Inventors: Taro Fukaya, Kenji Tsutsumi, Atsushi Yamazaki, Ichiro Yamanashi, Shuji Seki
  • Patent number: 8247204
    Abstract: Magnetic enrichment method, wherein the desired biological component is collected from a solution, which component is thereafter enriched in a liquid in such a manner that by means of the micro particles attached to the magnet or attached by means of at least one magnet at least one biological component is collected in a closed reactor vessel. Thereafter at least one biological component is enriched in such a manner that the desired component is released to the solution. The reactor unit is a closed vessel, wherein the prevailing conditions are controllable. The shape and the location of the magnet unit in the reactor unit are adjusted in a preferable manner to collect the desired biological component.
    Type: Grant
    Filed: October 20, 2004
    Date of Patent: August 21, 2012
    Assignee: BioControl Systems, Inc.
    Inventors: Matti Korpela, Kenneth Rundt
  • Patent number: 8216825
    Abstract: Methods and compositions for increasing the productivity of algae cultures by controlling grazers are described herein.
    Type: Grant
    Filed: March 7, 2011
    Date of Patent: July 10, 2012
    Inventor: Alex Yuan
  • Patent number: 8183009
    Abstract: An improved system for assaying expression of various markers, factors or antigens on the cultured cells for diagnostic purposes and for using such expression to monitor the applicability of certain candidate therapeutic or chemotherapeutic agents and the progress of treatment with those agents.
    Type: Grant
    Filed: January 20, 2010
    Date of Patent: May 22, 2012
    Assignee: Precision Therapeutics, Inc.
    Inventor: Paul L. Kornblith
  • Patent number: 8158411
    Abstract: Provided is a method of separating microorganisms from a sample including contacting the sample containing microorganisms with an inorganic ion exchange material such that the sample reacts with the inorganic ion exchange material, and contacting the reacted sample with a means for capturing microorganisms.
    Type: Grant
    Filed: August 20, 2007
    Date of Patent: April 17, 2012
    Assignee: Samsung Electronics Co., Ltd.
    Inventors: Kyu-youn Hwang, Sung-young Jeong, Joon-ho Kim
  • Patent number: 8092691
    Abstract: A method and device for separating particles from a liquid having particles therein, using a filter sized to permit the liquid to flow through pores, while retaining a substantial portion of the particles on the filter, with an absorbent layer contacting the back of the filter to facilitate liquid movement away from the particles.
    Type: Grant
    Filed: March 9, 2010
    Date of Patent: January 10, 2012
    Assignee: Univenture, Inc.
    Inventors: Ross O. Youngs, James Robert Cook