Removing Nucleic Acid From Intact Or Disrupted Cell Patents (Class 435/270)
  • Patent number: 11434519
    Abstract: A direct chemical lysis composition includes an assay compatible buffer composition and an assay compatible surfactant. When combined with a specimen storage composition, such compositions prevent undesired modifications to nucleic acid and proteins lysed from cells in the biological sample. Assays of samples from such compositions do not require expensive and time-consuming steps such as centrifugation and prolonged high temperature processing. The direct chemical lysis composition of the present invention permits direct nucleic acid extraction from the cells in the biological sample without the need to decant off the transport media or otherwise exchange the transport media with assay compatible buffers. There is no need to combine the sample with proteinase K or another enzyme to extract nucleic acids from the cells. A method for lysing cells to obtain target nucleic acid for assay and a kit for combining the direct chemical lysis composition with a sample are also contemplated.
    Type: Grant
    Filed: April 30, 2019
    Date of Patent: September 6, 2022
    Assignee: Becton, Dickinson and Company
    Inventors: Feng Yang, Sha-Sha Wang, Laurence Michael Vaughan, Michael Porter, Elaine Rose
  • Patent number: 11415494
    Abstract: A method of rapidly and completely rendering a tissue (11) rich in lipid droplets (12) transparent. The method comprises the following steps: providing a tissue sample (11) rich in lipid droplets (12) and immobilized with a hydrogel; performing a pre-transparency-rendering process on the tissue sample (11) to obtain a pre-processed sample; performing a transparency-rendering process on the pre-processed sample to obtain a transparency-rendered sample; and performing a post-transparency-rendering process on the transparency-rendered sample to obtain a final transparency-rendered sample (31). The method does not damage the fine structure of a biological tissue (11) and can significantly increase a depth of an optical image of the biological tissue (11). The method does not damage the fine structure of a biological tissue (11) and can significantly increase a depth of an optical image of the biological tissue (11).
    Type: Grant
    Filed: January 19, 2018
    Date of Patent: August 16, 2022
    Inventors: Xiaowei Li, Zhifeng Shao, Mengjie Lai, Jun Li, Daniel Czajkowsky
  • Patent number: 11332796
    Abstract: The present invention relates to a fast and efficient method of isolating nucleic acids in high yield and in high concentration from biological samples, using an aqueous two phase system without the need for instrumentation. The isolated nucleic acids can be used to facilitate the screening, prognosis and monitoring of disease progression.
    Type: Grant
    Filed: January 18, 2019
    Date of Patent: May 17, 2022
    Assignee: Phase Scientific International, Ltd.
    Inventor: Yin To Chiu
  • Patent number: 11332777
    Abstract: Disclosed herein are methods, reagent kits, and compositions for performing a bisulfite conversion of DNA directly from a biological sample including a patient's urine sample or a slide-mounted FFPE tissue sample. For example, a method of performing a bisulfite conversion of DNA can comprise certain preliminary steps for processing the biological sample and transferring a portion of the processed sample into a reaction vessel containing a bisulfite mixture. The method can further comprise heating the reaction vessel containing the biological sample and the bisulfite mixture at several heating temperatures and subsequently holding the reaction vessel at a holding temperature for a holding period. The method can also comprise certain bisulfite removal steps, desulfonation steps, and removal of the desulfonation solution. A final elution step can yield the converted DNA for further downstream sequencing and analysis.
    Type: Grant
    Filed: October 28, 2021
    Date of Patent: May 17, 2022
    Assignee: URIT Medical Electronic Co., Ltd.
    Inventors: Tom Cheng Xu, Kuikui Shen, Sally Li Shen, Jinlan Xu, Shilei Tang, Jusong Liang
  • Patent number: 11230729
    Abstract: Efficient harvesting of cells, cell fragments, free nuclei, and DNA material from female reproductive system is made possible by processing gelatinous part of cervical mucus. Rinsing may be used to separate the gelatinous part of cervical mucus from the remainder of the cervical mucus sample.
    Type: Grant
    Filed: February 17, 2019
    Date of Patent: January 25, 2022
    Inventor: Maya Tevlin
  • Patent number: 11071978
    Abstract: A tissue sample that has been removed from a subject can be properly fixed for evaluation using the disclosed transporter assembly for carrying a tissue sample and method for fixing an unfixed tissue sample. In one embodiment, the disclosed assembly includes a transport container, a fixative in the transport container, and a cooling device that reduces and/or maintains the temperature of the fixative to perform a pre-soaking process at a temperature of less than about 7° C. The pre-soaking process can, for example, be performed during sample transport or during extended periods of storage, such as over a weekend.
    Type: Grant
    Filed: July 25, 2017
    Date of Patent: July 27, 2021
    Assignee: Ventana Medical Systems, Inc.
    Inventors: Nathan Crum, Timothy Keller, Alastair Laing, Michael Otter, Vinnie Petre, Elizabeth Pigman, Taylor Shingler, Stacey Stanislaw, Benjamin Stevens
  • Patent number: 11051532
    Abstract: This disclosure describes methods for purifying protein, and more particularly to methods for purifying protein that minimize the development of undesirable odors and flavors in the purified protein and increase protein yield.
    Type: Grant
    Filed: September 21, 2018
    Date of Patent: July 6, 2021
    Assignee: Impossible Foods Inc.
    Inventors: Carl Allen Henderson, Jr., Ratnayake Mudiyanselage Dunilka Nishani Ratnayake, Ian Guiles Ronningen, Laila Dafik
  • Patent number: 11021505
    Abstract: The invention relates to an aqueous extract enriched with small RNA having a maximum length of 150 nucleotides, from plant material. The invention relates to an aqueous extract of plant material, enriched with small RNAs having a maximum length of 150 nucleotides, which can be obtained by such a method, as well as to the compositions comprising such an extract and to their cosmetic uses for combating the signs of skin aging and for improving the hydration of the skin.
    Type: Grant
    Filed: November 10, 2016
    Date of Patent: June 1, 2021
    Inventors: Elodie Oger, Valerie Lequoy, Frederique Portolan
  • Patent number: 10793920
    Abstract: The invention relates to a method of identifying a specific yeast species in patient tissue or body fluid. The method comprises the steps of extracting and recovering DNA of the yeast species from the patient tissue or body fluid, amplifying the DNA, hybridizing a probe to the DNA to specifically identify the yeast species, and specifically identifying the yeast species. The invention also relates to a method of identifying a yeast mycotoxin in patient tissue or body fluid. The method comprises the steps of extracting and recovering the yeast mycotoxin from the patient tissue or body fluid, contacting the yeast mycotoxin with an antibody directed against the yeast mycotoxin, and identifying the yeast myocotoxin. Both of these methods can be used to determine if a patient is at risk for or has developed a disease state related to a yeast infection, and to develop an effective treatment regimen for the patient.
    Type: Grant
    Filed: July 20, 2018
    Date of Patent: October 6, 2020
    Assignee: MYCODART, INC.
    Inventor: Dennis G. Hooper
  • Patent number: 10745763
    Abstract: The invention generally relates to a system for isolating or separating a target from a sample. In certain aspects, processes performed by the target capture system include introducing a plurality of magnetic particles, in which a plurality of the particles include at least one binding moiety specific to a target, into a sample to form at least one target/particle complex and applying a magnetic field to isolate the magnetic particle/target complexes from the sample. The process starts at inputting a sample into the system and ends at delivering a capture target or nucleic acids of the target into a container for further analysis.
    Type: Grant
    Filed: March 20, 2017
    Date of Patent: August 18, 2020
    Assignee: DNAE Group Holdings Limited
    Inventors: Ravil A. Sitdikov, Eddie W. Adams, Magdalena A. Torrance, David K. Aley, Erik J. Smith, Victor C. Esch
  • Patent number: 10641689
    Abstract: A device for preparing a glass slide specimen of cells has: a filter that has recesses, which capture target cells that are at least one of circulating tumor cells within blood or rare tumor cells within a body fluid, and pores that are formed in the recesses and that pass non-target cells therethrough; a glass slide that is superposed onto a surface of the filter, at a side at which the target cells are captured, and onto which the target cells are transferred; a cover member that is placed on a surface of the filter at a side opposite from the glass slide, and that seals in a buffer solution for immersing the target cells at interiors of the recesses; and a container into which the glass slide is immersed and in which is stored a preservation liquid that preserves the target cells that have been transferred onto the glass slide.
    Type: Grant
    Filed: June 16, 2017
    Date of Patent: May 5, 2020
    Inventors: Seichin Kinuta, Yoshiyuki Ichinosawa, Hayao Nakanishi, Seiji Ito
  • Patent number: 10619187
    Abstract: The present invention relates to compositions and methods for preserving and extracting nucleic acids from saliva. The compositions include a chelating agent, a denaturing agent, buffers to maintain the pH of the composition within ranges desirable for DNA and/or RNA. The compositions may also include a reducing agent and/or antimicrobial agent. The invention extends to methods of using the compositions of the invention to preserve and isolate nucleic acids from saliva as well as to containers for the compositions of the invention.
    Type: Grant
    Filed: November 7, 2016
    Date of Patent: April 14, 2020
    Assignee: DNA GENOTEK INC.
    Inventor: H. Chaim Birnboim
  • Patent number: 10597652
    Abstract: The invention provides pipette tip columns and automated methods for the purification of nucleic acids including plasmids. Nucleic acids can be purified from unclarified, clarified or partially-clarified cell lysates that contain cell debris. The columns typically include a bed of medium positioned in the pipette tip column, above a bottom frit and with an optional top frit. Plasmid preparation scales include miniprep, midiprep, maxiprep, megaprep and gigaprep.
    Type: Grant
    Filed: October 11, 2016
    Date of Patent: March 24, 2020
    Assignee: PHYNEXUS, INC.
    Inventors: Chris Suh, Carrie Loan Kim Huynh, Lee Hoang, Douglas T. Gjerde, Jonathan Michael Grambow
  • Patent number: 10508254
    Abstract: A method of inducing competence in cells, the method comprising contacting the cell with a composition comprising a quaternary ammonium compound including a silicon-containing functional group and a hydrocarbyl-saccharide compound.
    Type: Grant
    Filed: February 20, 2015
    Date of Patent: December 17, 2019
    Inventor: Jan Rogers
  • Patent number: 10436389
    Abstract: A system for processing objects to be cleaned that includes a processing vessel, and a storage vessel that includes an upper section for storing clean liquid and a lower section for storing dirty liquid. The upper section and lower section are in flow communication.
    Type: Grant
    Filed: June 22, 2015
    Date of Patent: October 8, 2019
    Inventor: Andrew Kegler
  • Patent number: 10435433
    Abstract: An improved, cost effective and shortened process of purification of the capsular polysaccharide of S. Pneumoniae, Group B Streptococcus, H. Influenzae, S. Typhoid and N. meningitidis is disclosed. The process includes a cocktail of enzyme treatment, tangential flow filtration, and multimodal chromatography purification. For Gram-negative bacteria, endotoxin removal process involves endotrap HD resin. This shortened process achieved the purity required by WHO/EP/BP for the use in human vaccine preparation, with simple steps and higher yield as compared to conventional processes. The steps of the process avoid use of organic solvents such as, for example, alcohol, phenol, and ultracentrifugation that are otherwise expensive and time consuming to perform, and/or health hazards for commercial use. This process disclosed is also simple, efficient, non-toxic, easy to scale-up, and environmentally friendly.
    Type: Grant
    Filed: June 29, 2018
    Date of Patent: October 8, 2019
    Assignee: Inventprise, LLC
    Inventors: Subhash V. Kapre, Anup K. Datta
  • Patent number: 10385406
    Abstract: Provided herein is technology for lung neoplasia screening and particularly, but not exclusively, to methods, compositions, and related uses for detecting the presence of lung cancer.
    Type: Grant
    Filed: March 28, 2017
    Date of Patent: August 20, 2019
    Assignees: EXACT SCIENCES DEVELOPMENT COMPANY, LLC, Mayo Foundation for Medical Education and Research
    Inventors: Hatim Allawi, Graham P. Lidgard, Maria Giakoumopoulos, David A. Ahlquist, William R. Taylor, Douglas Mahoney
  • Patent number: 10364427
    Abstract: There is described a method of extracting DNA and/or RNA from a cell or capsid, the method comprising contacting the cell or capsid with a composition comprising a quaternary ammonium compound including a silicon-containing functional group. The quaternary ammonium compound may be of general formula (I) or a derivative salt thereof wherein L is a linking group; each of R1, R2, R3, R4, R5 and R6 is independently selected from H or an optionally substituted alkyl, alkenyl, aryl or alkoxy group; and n is 0 or 1. A PCR promoting agent may be provided. The method may be one of 1 detecting and/or diagnosing a disease or medical condition.
    Type: Grant
    Filed: March 24, 2014
    Date of Patent: July 30, 2019
    Inventors: Benjamin David Cobb, Jan Rogers
  • Patent number: 10179922
    Abstract: The present invention provides methods of processing a biomass, e.g., for producing lipid and non-lipid materials, by inducing autolysis of a biomass. The biomass may be generated using any species of microorganism and any available biodegradable substrate, including waste materials. The lipid and non-lipid materials are present in two separate layers of the autolysate, and they can be used to generate valuable products such as biofuels and nutritional supplements. The present invention further provides a processing apparatus useful for practicing the methods of the present invention.
    Type: Grant
    Filed: June 19, 2014
    Date of Patent: January 15, 2019
    Inventor: Moustafa Ahmed El-Shafie
  • Patent number: 10173220
    Abstract: Tissue dissociators configured to disrupt a biological tissue sample are provided. Aspects of the tissue dissociators may include a housing having a distal end and a proximal end, a cutting blade positioned at the distal end of the housing and a tissue actuator configured to be displaced along a longitudinal axis within the housing. Also provided are methods of using the tissue dissociators, as well as kits including the tissue dissociators.
    Type: Grant
    Filed: August 4, 2015
    Date of Patent: January 8, 2019
    Inventors: Aaron J. Middlebrook, Smita Ghanekar, Brian David Warner, Albert Mach, David Vrane
  • Patent number: 10160965
    Abstract: Provided herein is materials and method relating to nucleic acids extraction and purification from biological samples. In particular, a pre-treatment buffer is used to facilitate the extraction and purification of nucleic acids from biological samples, more specifically, removing inhibitors and impurities from biological samples and resulting in a highly concentrated and purified nucleic acids preparation.
    Type: Grant
    Filed: May 14, 2015
    Date of Patent: December 25, 2018
    Assignee: GLC Biotechnology, Inc.
    Inventors: Yiding Liu, Baochuan Guo
  • Patent number: 10065186
    Abstract: Fluidic cartridges, and manufacture thereof, having a plurality of circuit element subtypes containing pneumatically operated diaphragm members, where the diaphragm materials are selected for yield point, chemical resistance, breathability and other properties individually according to the fluidic element subtype are provided. A process of in-situ edge-bonded decoupage for forming diaphragm members inside a cartridge, and fluidic circuits having diaphragm members as active and passive circuit elements, including pumps, valves, vents, waste receptacles, reagent reservoirs, and cuvettes with optical windows, where the material composition of each individual diaphragm member may be selected from an assortment of materials during manufacture are also provided.
    Type: Grant
    Filed: December 20, 2013
    Date of Patent: September 4, 2018
    Assignee: MICRONICS, INC.
    Inventors: Andrew Kolb, Isaac Sprague, Justin L. Kay, Matthew Scott Bragd, Martha Stone
  • Patent number: 9994840
    Abstract: A method of extracting DNA from a biological sample includes the steps of freezing a biological sample; mechanically breaking down the sample to produce a processed sample; mixing the processed sample with a lysis solution; and cycling the mixture through a series of pressure fluctuations to produce a resulting lysate. The method may further include the steps of exposing the lysate to a material that binds to DNA present in the sample and collecting the DNA from the sample using an elution solution. An apparatus for practicing the method includes a first segment for mechanically breaking down the sample; a second segment for pressurizing a mixture of the processed sample and lysis solution to produce a lysate; and wherein the first segment is fluidly coupled to the second segment. The apparatus may also include a third segment in which the lysate is exposed to the binding material.
    Type: Grant
    Filed: June 12, 2015
    Date of Patent: June 12, 2018
    Assignee: AG-Defense Systems, Inc.
    Inventors: Rex Bryan Dyer, Beth Susan Turnbull-Dyer, Kenneth L. Owen, John G. Schierer
  • Patent number: 9909118
    Abstract: This invention provides compositions and methods for rapid separation, isolation and purification of nucleic acids from biological samples using anionic exchange media. The method can utilize commercially available strong or weak anion exchanger materials with selected solutions of known ionic strength for adsorption and elution. The instant method is particularly advantageous as it permits the purification and identification of shorter fragments of nucleic acids from bodily fluids which, until now, had not been identified.
    Type: Grant
    Filed: April 30, 2015
    Date of Patent: March 6, 2018
    Assignee: Trovagene, Inc.
    Inventors: Samuil R Umansky, Hovsep S Melkonyan, Erik Meyer, William John Feaver
  • Patent number: 9897594
    Abstract: The present invention provides an assay method for a cell-containing body sample, the method comprising treating the sample under conditions whereby to cause cell lysis, preferably by means of a detergent; and subjecting the thus-generated lysed sample to conditions causing the cleavage of nucleic acid molecules. The invention additionally provides the use of nucleic acid cleavage conditions in enhancing a membrane assay, a device for carrying out such an assay, and a kit for use in the assay.
    Type: Grant
    Filed: October 31, 2006
    Date of Patent: February 20, 2018
    Assignee: AXIS-SHIELD ASA
    Inventors: Jostein Holtlund, Andrew Campbell, Morten Borch
  • Patent number: 9783799
    Abstract: Provided herein is technology related to processing samples of nucleic acids and particularly, but not exclusively, to methods for enriching samples for small nucleic acids, such as small circulating cell-free DNA.
    Type: Grant
    Filed: October 23, 2015
    Date of Patent: October 10, 2017
    Inventors: Dae Hyun H. Kim, Gerard J. Gundling, Herbert A. Marble, Mark W. Eshoo
  • Patent number: 9695413
    Abstract: An object of the present invention is to provide a technique for preparing RNA ready for an enzymatic reaction more easily than conventional techniques. The present invention provides a reagent for RNA extraction from a biological sample which contains an alkali metal salt and a surfactant.
    Type: Grant
    Filed: October 25, 2013
    Date of Patent: July 4, 2017
    Inventors: Sotaro Sano, Shigehiko Miyamoto, Jun Tomono, Hajime Hiratsuka
  • Patent number: 9487836
    Abstract: The invention relates to a method of identifying a specific yeast species in patient tissue or body fluid. The method comprises the steps of extracting and recovering DNA of the yeast species from the patient tissue or body fluid, amplifying the DNA, hybridizing a probe to the DNA to specifically identify the yeast species, and specifically identifying the yeast species. The invention also relates to a method of identifying a yeast mycotoxin in patient tissue or body fluid. The method comprises the steps of extracting and recovering the yeast mycotoxin from the patient tissue or body fluid, contacting the yeast mycotoxin with an antibody directed against the yeast mycotoxin, and identifying the yeast myocotoxin. Both of these methods can be used to determine if a patient is at risk for or has developed a disease state related to a yeast infection, and to develop an effective treatment regimen for the patient.
    Type: Grant
    Filed: January 26, 2015
    Date of Patent: November 8, 2016
    Assignee: Medical Service Consultation International, LLC
    Inventor: Dennis G. Hooper
  • Patent number: 9458494
    Abstract: Provided is a method for extracting nucleic acids from a wax-embedded sample, and use of particular solvents for removing wax from a wax-embedded sample for extracting, isolating and/or purifying nucleic acids from a crosslinked wax-embedded sample.
    Type: Grant
    Filed: June 14, 2011
    Date of Patent: October 4, 2016
    Assignee: QIAGEN GmbH
    Inventors: Markus Mueller, Thorsten Singer, Esther Rosenberg, Joerg Hucklenbroich
  • Patent number: 9322014
    Abstract: A system for enhancing a nucleic acid sample may include a one pump, a denaturing chamber; a microfluidic hydroxyapatite chromatography device configured for performing hydroxyapatite chromatography on the nucleic acid sample, a sample collector, and tubing connecting the pump with the denaturing chamber, the hydroxyapatite chromatography device and the sample collector such that the pump may be used to move the nucleic acid sample from the denaturing chamber to the hydroxyapatite chromatography device and then to the sample collector.
    Type: Grant
    Filed: November 20, 2013
    Date of Patent: April 26, 2016
    Assignee: Sandia Corporation
    Inventors: Victoria A. VanderNoot, Stanley Alan Langevin, Zachary Bent, Ronald F. Renzi, Scott M. Ferko, James L. Van De Vreugde, Todd Lane, Kamlesh Patel, Steven Branda
  • Patent number: 9057673
    Abstract: Methods for preparing RNA from ribonuclease-rich sources while avoiding RNA degradation are described. The lysis protocol for ribonuclease-containing samples is performed at high pH to accelerate cell lysis and with a reducing agent that inactivates ribonucleases (RNases) by reducing disulfide bonds essential for RNase activity. Samples are briefly incubated for up to five minutes at high pH followed by addition of a reagent to lower the pH to a level at which the RNA is stable. This method of RNA extraction has many advantages over existing methods of RNA preparation, including that cell lysis is efficient, RNases are rapidly inactivated, and sample incubation times are short (less than 5 minutes), which protects RNA from degradation. The lysing procedure is performed entirely in aqueous solution with no heating, precipitations, or buffer exchanges required. Thus, a quick, simple procedure for extracting RNA is provided, which can easily be automated.
    Type: Grant
    Filed: August 21, 2013
    Date of Patent: June 16, 2015
    Assignee: The Board of Trustees of the Leland Stanford Junior University
    Inventors: Anita Rogacs, Juan G. Santiago
  • Publication number: 20150140595
    Abstract: A set of paramagnetic particles synthesized by co-precipitation methods wherein an alkaline hydroxide solution is mixed with a metal salt solution. The alkaline hydroxide features ammonium hydroxide, potassium hydroxide, sodium hydroxide, or mixtures thereof. The metal salt solution features at least one ferrous salt and at least one tetravalent metal salt selected from Group 4 elements of the Periodic Table. The concentration of the ferrous salt is equal to or greater than the concentration of the tetravalent metal salt. The paramagnetic particles may be used for bioprocessing via magnetic fields. Bioprocessing, for example, may include purifying, concentrating, or detecting biomolecules of interest (e.g., nucleic acids, carbohydrates, peptides, proteins, other organic molecules, cells, organelles, microorganisms, viruses, etc.), or other magnetic field-based processes common to applications in separation science, diagnostics, molecular biology, protein chemistry, and clinical practice.
    Type: Application
    Filed: May 22, 2013
    Publication date: May 21, 2015
    Inventor: Joseph Gerard UTERMOHLEN
  • Publication number: 20150132749
    Abstract: The invention generally relates to methods and kits for isolating nucleic acids from an organism. In certain embodiments, methods of the invention involve contacting a plurality of lytic enzymes to an organism, thereby lysing a cell wall of the organism to release the nucleic acid, and introducing at least one agent to separate the nucleic acid from the lysed cells, thereby isolating the nucleic acid.
    Type: Application
    Filed: January 12, 2015
    Publication date: May 14, 2015
    Inventor: John Kevin Henkhaus
  • Patent number: 8993306
    Abstract: A method for at least partially separating viral and/or prokaryotic nucleic acids from eukaryotic nucleic acids from a biological sample includes re-suspending the cells in the presence of a chelating agent, lysis of the cells by chemical lysis, and/or mechanical lysis, neutralizing the cell lysate and separating the precipitated eukaryotic nucleic acids and obtaining the viral and/or prokaryotic nucleic acids. A kit includes a re-suspension buffer with chelating agent and optionally a saccharide and RNAse, lysis buffer including at least one base and a detergent and neutralizing buffer for at least partially separating viral and/or prokaryotic nucleic acids from eukaryotic nucleic acids from a biological sample. The acid constant of the weak anion defines the pH value and kosmotropic property of the salt (cation+anion) in conjunction with the detergent, which determines the protein solubility and precipitation.
    Type: Grant
    Filed: April 7, 2011
    Date of Patent: March 31, 2015
    Assignee: Greiner Bio-One GmbH
    Inventor: Walter Rudorfer
  • Patent number: 8986976
    Abstract: The present disclosure provides a method to isolate natural & artificial nucleic acids like deoxyribonucleic acid (DNA), ribonucleic acid (RNA) and peptide nucleic acid (PNA) from a solid or liquid sample using cotton. The cotton packed is such that, a solution containing nucleic acids passes through it and the nucleic acids in solution are bound to the cotton in a medium optimal for binding. The nucleic acids are bound to cotton in such a way that, the bound nucleic acids can withstand multiple washes with liquid comprising water and gets eluted in an aqueous buffer, with which eluted nucleic acids can be directly used for amplification using PCR or for any other biochemical or molecular biology needs.
    Type: Grant
    Filed: January 6, 2011
    Date of Patent: March 24, 2015
    Assignee: Bigtec Private Limited
    Inventors: Phani Kumar Pullela, Mulakkapurath Narayanan Manoj, Santhosh Kumar Gandhavalla, Mitchell Preetham Pinto, Chandrasekhar Bhaskaran Nair, Pillarisetti Venkata Subbarao
  • Publication number: 20150010953
    Abstract: Provided herein is a method for producing a population of oligonucleotides that has reduced synthesis errors. In certain embodiments, the method comprises: a) obtaining an initial population of hairpin oligonucleotide molecules that each comprise a double-stranded stem region and a loop region; b) contacting the double-stranded region of the hairpin oligonucleotide molecules with a mismatch binding protein; and c) eliminating any molecules that bind to the mismatch binding protein, thereby producing a population of oligonucleotides that has reduced synthesis errors. A kit and a composition for performing the method are also provided.
    Type: Application
    Filed: July 3, 2013
    Publication date: January 8, 2015
    Inventors: Derek Lee Lindstrom, Jeffrey R. Sampson, Daniel E. Ryan
  • Publication number: 20150004675
    Abstract: The present invention relates to a method for recovering an aqueous phase comprising biomolecules dissolved therein from a multiphasic mixture, comprising at least said aqueous phase and a further liquid phase which is immiscible with said aqueous phase wherein said further liquid phase comprises at least one hydrocarbon compound. The invention further relates to the use of a hydrophilic filtering material, a device comprising such a filtering material or a kit comprising said device for recovering an aqueous phase comprising biomolecules dissolved therein from a mixture of said aqueous phase and at least one hydrocarbon compound comprising further liquid phase which is immiscible with said aqueous phase.
    Type: Application
    Filed: December 4, 2012
    Publication date: January 1, 2015
    Inventors: Jörg Hucklenbroich, Frank Narz
  • Publication number: 20140380527
    Abstract: The present invention relates to isolated polypeptides having lysozyme activity and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.
    Type: Application
    Filed: November 23, 2012
    Publication date: December 25, 2014
    Inventor: Kirk Matthew Schnorr
  • Publication number: 20140329703
    Abstract: The present invention provides improved methods and compositions for RNA isolation. In particular embodiments the present invention concerns the use of methods and compositions for the isolation of full-length RNA from fixed tissue samples. The present invention provides methods for digesting and extracting RNA from a fixed tissue sample.
    Type: Application
    Filed: March 12, 2014
    Publication date: November 6, 2014
    Applicant: Applied Biosystem, LLC
    Inventors: Richard CONRAD, Emily Zeringer
  • Patent number: 8852919
    Abstract: A microfluidic apparatus, method, and associated applications utilize and apply to a formalin-fixed paraffin-embedded (FFPE) tissue sample and performing a liquid-liquid extraction to remove the paraffin from the tissue sample prior to a nucleic acid purification step. A microfluidic device includes a dedicated liquid-liquid extraction process vessel, a nucleic acid purification process component, and a nucleic acid amplification reactor. A liquid-liquid extraction and nucleic acid purification kit includes a microfluidic device capable of performing both a liquid-liquid extraction process and a nucleic acid purification process, including a dedicated liquid-liquid extraction process vessel, an immiscible liquid or a precursor phase thereof disposed in the vessel, a nucleic acid purification process component, a nucleic acid amplification reactor fluidically, and a supply of reagents suitable to enable the liquid-liquid extraction process and the nucleic acid purification process.
    Type: Grant
    Filed: November 16, 2012
    Date of Patent: October 7, 2014
    Assignee: Rheonix, Inc.
    Inventor: Peng Zhou
  • Publication number: 20140220585
    Abstract: Collection devices and kits for biological sample collection include a biologic sample collection device having a hydrophilic swab matrix that includes a modified polycaprolactone (PCL). Methods of production and use thereof are also described herein. The biologic sample collection devices, kits and methods described herein are used to collect a biologic sample (e.g., blood, buccal cells, etc.) and to enable extraction of nucleic acids (e.g., DNA) from that biologic sample so that the nucleic acids can be analyzed (e.g., sequencing and subsequent analyses of DNA).
    Type: Application
    Filed: April 18, 2014
    Publication date: August 7, 2014
    Applicant: Diomics Corporation
    Inventors: Jeff Morhet, Thomas Kindt, Franco Ferrari, Vasana Maneeratana, Frederic Zenhausern
  • Publication number: 20140212871
    Abstract: Methods for extracting high quality nucleic acids from a heterogenous collection of nucleic acid-containing materials from a biological sample are disclosed. The heterogenous collection of nucleic-acid containing materials may contain cells or microvesicles, or both. The extractions obtained by the methods described herein are characterized by high yield and high integrity, making the extracted nucleic acids useful for various applications in which high quality nucleic acid extractions are preferred, e.g., a diagnosis, prognosis, or therapy evaluation for a medical condition.
    Type: Application
    Filed: May 11, 2012
    Publication date: July 31, 2014
    Inventors: Wayne Comper, Leileata M. Russo, Johan Karl Olov Skog
  • Publication number: 20140212885
    Abstract: The application provides a method of reducing the DNA content of a protein preparation or a culture broth from a filamentous fungal host cell using an endogenous filamentous fungal host DNase activity.
    Type: Application
    Filed: September 20, 2012
    Publication date: July 31, 2014
    Applicant: Danisco US Inc.
    Inventors: Katherine M. Hoffmann, Douglas Ko, Michael Ward
  • Publication number: 20140206009
    Abstract: A method is provided for purifying nucleic acid from a sample in a microfluidic device. The method can be used to purify nucleic acids from any source known in the art that comprises nucleic acids, such as prokaryotic or eukaryotic organisms, viruses, cell, tissues, organs, etc. In a specific example, the tissue is whole blood. The method for purifying nucleic acid may run fully automated in the microfluidic device.
    Type: Application
    Filed: March 26, 2014
    Publication date: July 24, 2014
    Applicant: RHEONIX, INC.
    Inventors: Lincoln C. Young, Peng Zhou, Gwendolyn Spizz, Rubina Yasmin
  • Publication number: 20140194313
    Abstract: The present invention relates to a microfluidic device for extracting and isolating DNA from cells. The device includes a support having an inlet port for receiving a sample containing a cell, an outlet port for dispensing DNA isolated from the cell, and a microfluidic channel disposed within the support and extending from the inlet port to the outlet port. The microfluidic channel includes a micropillar array, an inflow channel disposed between the inlet port and the micropillar array, and an outflow channel disposed between the micropillar array and the outlet port. The micropillar array includes micropillars spatially configured to entrap, by size exclusion, the cell, to immobilize DNA released from the cell, and to maintain the immobilized DNA in elongated or non-elongated form when hydrodynamic force is applied to the microfluidic channel. Systems and methods of making and using the device are also provided herein.
    Type: Application
    Filed: June 6, 2012
    Publication date: July 10, 2014
    Inventors: Harold G. Craighead, Juraj Topolancik, Harvey Tian, Christopher Wallin
  • Publication number: 20140170664
    Abstract: A heating mechanism for use in DNA applications such as DNA amplification, extraction and sterilization is provided. Nanoparticles having photo-thermal properties are put in contact with a reaction mixture and irradiated with an activation light beam to activate these photo-thermal properties, thereby releasing heat. Nanoparticles of several types may be used. Use of the same nanoparticles or of different one to monitor the reaction using a different light beam is also presented.
    Type: Application
    Filed: July 16, 2013
    Publication date: June 19, 2014
    Inventors: Philip ROCHE, Andrew Kirk, Lenore Beitel, Miltiadis Paliouras, Mark Trifiro, Vamsy Chodavarapu, Mohamed Najih, Joachim Thiemann
  • Publication number: 20140162347
    Abstract: An apparatus adapted to extract an ingredient from a fluid sample by bidirectional transferring of an aliquot of fluid having magnetic particles therein, said apparatus comprising: an open compartment operable at a constant ambient pressure; a closed compartment having a closed pressure, and an upper zone containing an air pocket adapted to generate internal positive and negative pressure; an intermediate transferring-retaining compartment adapted to temporarily retain a fraction of said aliquot of fluid; a flow barrier member adapted to inhibit a flow of said aliquot of fluid when said closed pressure is substantially equal to said ambient pressure and to allow said flow of fluid when said closed pressure is substantially different from said ambient pressure, the pressure differential controllable by a thermo member; a capturing zone adapted to allow magnetic particles in the fluid to be attracted in response to a magnetic force applied.
    Type: Application
    Filed: July 5, 2013
    Publication date: June 12, 2014
    Inventor: Beno ALSPEKTOR
  • Patent number: 8744163
    Abstract: Systems, devices, and methods for removing areas of tissue are described. A programmable laser may remove precise areas of tissue while the tissue remains substantially frozen. The laser is programmed in part by analyzing a reference image of a representative tissue section. A software program may receive digital images of test slices. Areas of interest in the image may be selected by a user. The software program can then create and send cut instructions to the programmable laser. The laser may be configured to make perforated cuts to remove the area of interest without melting the removed section.
    Type: Grant
    Filed: February 8, 2011
    Date of Patent: June 3, 2014
    Assignee: International Genomics Consortium
    Inventor: Scott Morris
  • Publication number: 20140141424
    Abstract: An analyte is separated from a fluid sample by introducing the sample into a cartridge having a sample port and a first flow path extending from the sample port. The first flow path includes an extraction chamber containing a solid support for capturing the analyte from the sample. The cartridge has a second flow path for eluting the captured analyte from the extraction chamber, the second flow diverging from the first flow path after passing through the extraction chamber. The sample is forced to flow through the extraction chamber and into a waste chamber, thereby capturing the analyte with the solid support as the sample flows through the extraction chamber. The captured analyte is then eluted from the extraction chamber by forcing an elution fluid to flow through the extraction chamber and along the second flow path.
    Type: Application
    Filed: October 23, 2013
    Publication date: May 22, 2014
    Inventors: FARZAD POURAHMADI, William McMillan, Jesus Ching, Ronald Chang, Lee A. Christel, Gregory T.A. Kovacs, M. Allen Northrup, Kurt E. Petersen
  • Patent number: 8722337
    Abstract: The instant invention provides an intergrated kit with solutions and instruments to isolate various biological samples from biological samples. The kit enables an user to preserve specimens and isolate biomolecules with features and benefits of high quality, easy, fast, no toxicity, safe to user and environment, low demanding, cost-effective, reducing waste, saving nature resources and protecting environment, and leads to a low-carbon and Green economy in preparation of specimens. The integrated kit enables the user to extract biomolecules including DNA/ccfDNA, Large RNA/mRNA/ccfRNA, Small RNA/miRNA/ccfmiRNA, Protein, Lipid, Carbohydrates, and Metabolite using one seamless procedure.
    Type: Grant
    Filed: August 6, 2013
    Date of Patent: May 13, 2014
    Assignee: BexMart
    Inventors: Bob Han, Eric Han, Xiaohui Xiong, Xiaoliang Han