Abstract: Compositions and methods for modifying genomic DNA sequences are provided. The methods produce double-stranded breaks (DSBs) at pre-determined target sites in a genomic DNA sequence, resulting in mutation, insertion, and/or deletion of DNA sequences at the target site(s) in a genome. Compositions comprise DNA constructs comprising nucleotide sequences that encode a Cms1 protein operably linked to a promoter that is operable in the cells of interest. The DNA constructs can be used to direct the modification of genomic DNA at pre-determined genomic loci. Methods to use these DNA constructs to modify genomic DNA sequences are described herein. Additionally, compositions and methods for modulating the expression of genes are provided.

Abstract: The present invention relates to an early warning method before the occurrence of aflatoxin contamination.

Abstract: This application relates to methods and materials for providing a benefit to a seed or seedling of an agricultural plant (e.g., an agricultural grass plant), or the agricultural plant derived from the seed or seedling. For example, this application provides purified bacterial populations that include novel seed-origin bacterial endophytes, and synthetic combinations of seeds and/or seedlings (e.g., cereal seeds and/or seedlings) with heterologous seed-derived bacterial endophytes.

Abstract: Animal and poultry solid waste material are treated with a preferred lactobacillus organism, preferably plant derived, in a liquid medium, preferably non-chlorinated water for time sufficient to reduce odor a significant amount.

Abstract: A method for decolorizing and deproteinizing brown algae polysaccharides belongs to the field of deep processing of brown algae. The method combines ultraviolet (UV) with hydrogen peroxide (H2O2), including the following steps: extracting dried and pulverized brown algae by hot water to obtain brown algae polysaccharide, dissolving the brown algae polysaccharide in an aqueous solution containing H2O2 and irradiating under ultraviolet light, wherein the mass concentration of the brown algae polysaccharide is 2.5-10.0 mg/mL, the concentration of the H2O2 is 25-150 mmol/L, and the UV irradiation time is 1.0-2.0 h, so as to deproteinize and decolorize the brown algae. The invention does not use acids, bases and organic solvents, which is green with no pollution, simple in operation, safe, economical and time-saving.

Abstract: The invention relates to a process for enzymatic degumming of unrefined triglyceride oil, said process comprising the following successive steps: (a) providing an unrefined triglyceride oil having a phosphorus content of at least 100 mg per kg of unrefined triglyceride oil; (b) combining the unrefined triglyceride oil with water, an acid and a phospholipase to produce an oil-in-water emulsion having a pH in the range of 2.5 to 4.5; said phospholipase being selected phospholipase A1, phospholipase A2 and combinations thereof; (c) keeping the emulsion at a temperature of 20-90° C. for at least 10 minutes; (d) introducing a base into the emulsion; and (e) separating degummed triglyceride oil from the emulsion. This enzymatic degumming process is extremely effective in removing phospholipids, including non-hydratable phospholipids (NHP), from unrefined vegetable oils and produces degummed vegetable oil in high yield.

Abstract: This disclosure relates to compositions, kits and methods for non-surgical reduction of localized subcutaneous fat such as that associated with a cosmetic fat accumulation. The methods employ compositions having specific concentrations of a salt of deoxycholic acid which provides a superior fat cell necrosis with modest adverse effects. Examples of localized subcutaneous fat are found in the submental area, in particular under the chin.

Abstract: The present invention is a kit to be used for obtaining Adipose tissue-based stem cells, which especially is suitable for obtainment of Adipose tissue-based stem cells, which comprises a reservoir (1) through which the entrance of the adipose tissue, that is taken from a predetermined area of the individual, into the kit; characterized by comprising a first housing (2), which is located on the said reservoir (1) and enables the transmission of the adipose tissue into the kit, a second housing (3), which is located on the said reservoir (1) and which enables the second injector (40) to be positioned outside the reservoir (1) and in a perpendicular position to the reservoir (1), a third housing (4) which enables the third injector (50) to be positioned outside the reservoir (1) and in a perpendicular position to the reservoir (1), an empty body (6) which is fixed inside the reservoir (1), a first inlet (7) which enables the transmission of the adipose tissue into the body (6) by pushing of the injector, a passa

Abstract: A fat cutter and an integrated machine for preparation of in vitro fine-particle fat are provided. The fat cutter includes a fat inlet channel, a fat outlet channel, at least one set of blades disposed between an outlet of the fat inlet channel and an inlet of the fat outlet channel, and a housing that seals a space in which the outlet of the fat inlet channel, the inlet of the fat outlet channel, and the blades are located. A fat inlet of the fat inlet channel is connected to a large-diameter liposuction tube by a liposuction channel, and a fat outlet of the fat outlet channel is connected to a high-negative pressure device. The outlet of the fat inlet channel and the inlet of the fat outlet channel are oppositely provided, and a gap therebetween matches the thickness of the blades.

Abstract: The present invention provides an active substance of Morchella, its use and a composition thereof for improving disturbance of reproductive function, especially, for manufacturing a pharmaceutical composition to improve disturbance of reproductive function induced by obesity or metabolic syndrome. The composition with the active substance of Morchella can effectively improve the structural integrity of testicular tissues and sperm, increase testosterone levels in the blood, and reduce the oxidation stress in sperms.

Abstract: The disclosure provides methods to isolate genome or chromosome level structural information from preserved samples. In some cases, samples preserved under conditions where long-range nucleic acid information is believed to be irreparably lost, such as FFPE samples, are treated to recover nucleic acid-protein complexes stabilized as part of the sample preservation process. The complexes are processed so as to recover information regarding which nucleic acids are bound to a common complex, and the information is used to recover genomic structural information.

Abstract: Isolated extracellular matrix from marrow adherent stromal cells and their descendents, which stimulates the growth and survival of a number of different neural cell types, is provided, along with methods for preparation and uses.

Abstract: The invention relates to processes of multi-stage hydrolysis where different enzyme compositions are added in at least two stages of hydrolysis. In a first stage, a first enzyme composition of one or more cellulolytic enzymes and at least one of an oxidoreductase and an AA9 polypeptide having cellulolytic enhancing activity is added, followed by a latter stage in which a second enzyme composition comprising one or more cellulases is added. Also provided are processes for obtaining hydrolysis products and fermentation products using processes of the invention.

Abstract: A cell culture system including a silk fibroid scaffold, culture media, and salivary gland cells. The salivary gland cells grown in the tissue culture system have physiological and morphological features like those of in vivo salivary gland cells. The cell culture system can be used to produce a salivary tissue-specific extracellular matrix capable of inducing differentiation of salivary gland cell precursors into salivary gland cells.

Abstract: The present invention pertains to methods of extracting cardiac glycosides from cardiac glycoside containing plant material, such as Nerium oleander, through use of aloe. It further provides for compositions resulting from such extractions, pharmaceutical compositions, cosmetic compositions, and methods of treating skin conditions.

Abstract: The invention concerns a powder consisting of 0.5 to 0.8% by weight of water and a dry material consisting of 88 to 95% by weight of an acerola fruit dry extract and 5 to 12% by weight of magnesium, calcium, zinc, sodium or potassium hydroxide or carbonate, the method of preparing same and the applications thereof for replacing ascorbic acid and/or the derivatives of same in food products.

Abstract: The invention concerns a powder consisting of 0.5 to 8% by weight of water and a dry material consisting of 88 to 95% by weight of an acerola fruit dry extract and 5 to 12% by weight of magnesium, calcium, zinc, sodium or potassium hydroxide or carbonate, the method of preparing same and the applications thereof for stabilizing a natural dye.

Abstract: Methods for detecting HAV in a biological sample are provided, comprising amplifying a target nucleic acid comprising the sequence of HAV in a reaction mixture. The reaction mixture comprises a biological sample which may contain the target nucleic acid and set of oligonucleotides. The invention also provides kits for the detection of HAV.

Abstract: The present invention relates to isolated polypeptides having catalase activity and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.

Abstract: Disclosed are methods and compositions for amplification of genetic material, including isothermal WGA of single cells.

Abstract: The present invention relates to a cross-linked sodium hyaluronate gel for tissue filler for plastic surgery and the preparation method thereof. The alkaline solution of sodium hyaluronate reacts with the long chain alkane containing epoxy group and the cross-linking agent containing epoxy group for 2˜5 hours at 35° C.˜50° C. to produce cross-linked sodium hyaluronate, then washed, gelled and sterilized, to prepare the gel. Among which, the molar ratio of sodium hyaluronate:cross-linking agent containing epoxy group:long chain alkane containing epoxy group is 10:4˜1:1˜4; the number of carbon atoms of the said long chain alkane containing epoxy group is 6 to 18. The gel prepared in the present invention can, on one hand, effectively enhance the resistance to enzymolysis to become more stable, and on the other hand, maintain the excellent biocompatibility of sodium hyaluronate without affecting its injectability.

Abstract: The present disclosure provides methods and compositions, including a placental extract, for inducing and/or modulating angiogenesis; methods of identifying modulators of angiogenesis, and assays for identifying modulators of angiogenesis. The present disclosure also provides methods of making a composition, including a placental extract that can induce and/or modulate angiogenesis.

Abstract: Provided herein are devices and methods for the capture or isolation of a biomarker from a biological sample. In several embodiments, the device comprises a loading region, a filter material, and a receiving region. In particular, in several embodiments, biological fluid is passed from the loading region through the filter material and into the receiving region, thereby resulting in capture or isolation of a biomarker.

Abstract: Provided herein are devices and methods for the capture or isolation of a biomarker from a biological sample. In several embodiments, the device comprises a loading region, a filter material, and a receiving region. In particular, in several embodiments, biological fluid is passed from the loading region through the filter material and into the receiving region, thereby resulting in capture or isolation of a biomarker.

Abstract: A unitary apparatus for isolating cells from adipose tissue including a lipid separation processor with a dispersing head equipped with a plurality of ports and a digestion chamber for dissociation of the constituent cells disposed in adipose tissue. The lipid separating apparatus is useful for the separation of lipids and adipocytes from a mixed cell population. A cell seeding chamber may be attached to the cell isolation apparatus. The components of the apparatus may be packaged in modular kit form.

Abstract: The present invention relates to a process wherein organic material derived from plant and animal material is processed to recover nutritional elements. In particular, there is provided a process for releasing nutritional elements from plant and animal material comprising the steps of treating the material with one or more enzymes to digest said material under appropriate conditions and separating the resulting liquid hydrolysate from the undigested material.

Abstract: Disclosed herein is an extract obtainable from Trigonella foenum-graecum. In particular, the extract is obtainable by a process comprising the steps of preparing a mixture of a plant material obtained from Trigonella foenum-graecum and liquid, incubating said mixture for at least 3 hours, heating of said mixture, and recovering a liquid extract from mixture. The extract is useful in the manufacture of various types of compositions, such as pharmaceutical compositions, disinfectant or preservation agents. The pharmaceutical may be used for the treatment or prevention of an inflammatory disease or an infectious condition.

Abstract: Disclosed is an antibacterial composition comprising titanium oxide particles immobilized with an antibody having affinity and cognitive power to a microorganism of interest, and a method for sterilizing the microorganism by using the same. In particular, the present invention relates to a method for preparing functional titanium oxide particles capable of recognizing a microorganism or a virus of interest, and a method for selectively and efficiently sterilizing the same by using the functional titanium oxide particles, and not for randomly sterilizing microorganisms or viruses by using conventional titanium oxide particles having no recognition power to a microorganism or a virus of interest.

Abstract: The invention provides the invention provides compositions and methods for the enzymatic treatment (“bleaching” or “de-colorizing”) of chlorophyll-comprising compositions, e.g., algae preparations, chlorophyll-containing or chlorophyll-contaminated feeds, foods or oils, for example, vegetable oils, including oils processed from oilseeds, such as canola (rapeseed) oil or soybean oil, or oil fruits, such as palm oil. In one aspect, the invention provides methods using a chlorophyllase enzyme for the enzymatic hydrolysis of chlorophyll in an algae, an animal (e.g., a fish) or plant preparation, a food or an oil. In one aspect, the chlorophyllase is immobilized onto a silica. The invention also provides compositions of manufacture and detergents.

Abstract: The present invention includes methods, devices and systems for isolating a nucleic acid from a fluid comprising cells. In various aspects, the methods, devices and systems may allow for a rapid procedure that requires a minimal amount of material and/or results in high purity nucleic acid isolated from complex fluids such as blood or environmental samples.

Abstract: The invention relates to processes and biocatalysts for producing ethanol and other useful products from biomass and/or other materials. Initial processing of lignocellulosic biomass frequently yields methylglucuronoxylose (MeGAX) and related products which are resistant to further processing by common biocatalysts. Strains of Enterobacter asburiae are shown to be useful in bioprocessing of MeGAX and other materials into useful bioproducts such as ethanol, acetate, lactate, and many others. Genetic engineering may be used to enhance production of desired bioproducts.

Abstract: Presented herein are methods of using cell wall degrading enzymes for recovery of internal lipid bodies from biomass sources such as algae. Also provided are algal cells that express at least one exogenous gene encoding a cell wall degrading enzyme and methods for recovering lipids from the cells.

Abstract: This invention relates to a sterilization agent for livestock bedding or a composting accelerator for organic waste or livestock bedding, comprising at least one Bacillus coagulans thermophilic bacterium or a combination of the at least one Bacillus coagulans thermophilic bacterium and at least one Bacillus subtilis mesophilic bacterium, as an active ingredient, wherein the Bacillus coagulans thermophilic bacterium as a preferable bacterium is viable at a temperature of 40 to 60° C. and the Bacillus subtilis mesophilic bacterium is viable at a temperature of 30 to 50° C., and the mesophilic bacterium also has a sterilizing effect on harmful bacteria in bedding; and to a method for sterilizing livestock bedding or a method for accelerating composting of organic waste or livestock bedding by using these agents each as an active ingredient.

Abstract: The invention provides isolated fungal endophytes and synthetic combinations thereof with host grass plants. Methods for inoculating grass plant with the endophytes, for propagating the grass-endophyte combinations, and for producing feeds and biofuels from grass-endophyte combinations are also provided.

Abstract: A process for the purification of plasmid DNA, the process comprising treating an aqueous composition containing plasmid DNA with a polypeptide obtained from an NST1 phage to digest colanic acid and separating the plasmid DNA from the treated aqueous composition.

Abstract: Decellularized tissue-derived extracellular matrices (ECM) and methods of generating and using same are provided. The method of generating a decellularized matrix includes the steps of: (a) subjecting the tissue to washes and a hypertonic buffer; (b) subjecting the tissue to an enzymatic proteolytic digestion with an enzyme such as trypsin; and (c) removing all cellular components from the tissue using a detergent solution which includes Triton-X-100 and ammonium hydroxide. Specifically, there is provided a decellularized myocardium-derived matrix which is completely devoid of all cellular components and hence non-immunogenic in a subject, exhibits suitable structural and mechanical properties for cardiac tissue engineering or replacement therapy of damaged cardiac tissue, and is capable of remodeling upon seeding of cells.

Abstract: Whole corn kernels or particles thereof are enzymatically disassembled. The method can produce a solid starch fraction, a solid pericarp fraction, and a liquid fraction. A high purity starch solids product can be provided suitable for use as a feedstock in other chemical processes.

Abstract: The present disclosure provides colorant compounds and methods of isolation of the colorant compounds derived from a reaction of genipin and an amine. The colorant compositions comprise purified compounds (e.g., a purified polymer or a purified dimer) obtained from multiple fractioning by chromatography of the reaction resulting material. The purified polymer or dimer can be used as a colorant by itself or in combination with another colorant for imparting color to a food, a drug, a cosmetic, a medical device, and textile products.

Abstract: This invention relates to phytases, polynucleotides encoding them, uses of the polynucleotides and polypeptides of the invention, as well as the production and isolation of such polynucleotides and polypeptides. In particular, the invention provides polypeptides having phytase activity under high temperature conditions, and phytases that retain activity after exposure to high temperatures. The phytases of the invention can be thermotolerant and/or thermostable at low temperatures, in addition to higher temperatures. The phytases of the invention can be used in foodstuffs to improve the feeding value of phytate rich ingredients. The phytases of the invention can be formulated as foods or feeds or supplements for either to, e.g., aid in the digestion of phytate. The foods or feeds of the invention can be in the form of pellets, liquids, powders and the like.

Abstract: The present invention relates to isolated polypeptides having lysozyme activity and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.

Abstract: The invention relates to applications of the cellulase Cel5H of Saccharophagus degradans and its homologues, functional fragments and/or variants and engineered forms thereof, in the context of recombinant, more particularly solventogenic microorganisms, more particularly C. acetobutylicum. The invention also characterizes a novel domain of the Cel5H cellulase with a putative cellulose-binding module function, and its uses in chimeric proteins for depolymerization of cellulose containing substrates.

Abstract: The present invention provides methods of processing a biomass, e.g., for producing lipid and non-lipid materials, by inducing autolysis of a biomass. The biomass may be generated using any species of microorganism and any available biodegradable substrate, including waste materials. The lipid and non-lipid materials are present in two separate layers of the autolysate, and they can be used to generate valuable products such as biofuels and nutritional supplements. The present invention further provides a processing apparatus useful for practicing the methods of the present invention.

Abstract: A process for the recovery of betaine from a raw material consisting essentially of molasses. The process includes a demineralisation step, a conversion step and a separation step. In the demineralisation step the overall amount of salts in the molasses is brought to a level lying below 2 wt. % (on overall dry matter). In the conversion step the molasses is subjected to the action of a fructan-forming enzyme, to form a fructan-containing molasses (fructan-molasses). Finally in the separation step, the fructan-molasses is subjected to a chromatographic separation, thereby obtaining a betaine-containing fraction. Whereby the demineralisation step is executed prior to the separation step and whereby demineralisation step may be executed prior to, during, or subsequent to the conversion step. The raw material may alternatively contain or consist essentially of thick juice.

Abstract: The present application relates to systems and methods for processing organic material. The methods may include extraction of biochemical nutrients from organic material, such as food scraps. The method can include comminuting the organic material to form a slurry from components comprising liquid and organic material; combining the slurry with microorganisms, such as a yeast, under aerobic conditions to form a mixture of the slurry and yeast; aerating the mixture; and forming a biomass and a nutrient-rich broth, in which the biochemical nutrients are stabilized and anabolized. The systems may, in some embodiments, be configured to perform the methods of processing organic materials.

Abstract: The present disclosure provides a method for pre-treating non-wood lignocellulosic material containing less than 5 % (w/w) starch or sugar in a process for production of ethanol from lignocellulose, comprising the steps of: adding organic acid or organic acid-producing bacteria to the lignocellulosic material; storing the lignocellulosic material in the presence of the organic acid for a period of at least two weeks in an atmosphere of less than 5% oxygen to obtain organic acid-impregnated material; and heating the organic acid-impregnated material at a temperature of at least 160° C. to obtain pre-treated lignocellulosic material, wherein no, or substantially no, inorganic acid or base, including SO2, is added in the method.

Abstract: A unitary apparatus for isolating cells from adipose tissue including a lipid separation processor with a dispersing head equipped with a plurality of ports and a digestion chamber for dissociation of the constituent cells disposed in adipose tissue. The lipid separating apparatus is useful for the separation of lipids and adipocytes from a mixed cell population. A cell seeding chamber may be attached to the cell isolation apparatus. The components of the apparatus may be packaged in modular kit form.

Abstract: The invention relates to a method for recovering of oil from plant seeds characterized in that a) an aqueous solution containing one or more cellulolytic and/or lipolytic and/or pectinolytic and/or proteolytic enzyme(s) and/or phytase is sprayed onto the seed, b) the thus obtained seed is directly supplied to a one-stage or multistage pressing in a way known per se, optionally coupled to an extraction, and c) the oil is recovered in a way known per se and optionally further processed, and the use of the method, particularly in the production of edible oil or biodiesel.

Abstract: Nutraceutical compositions for improved administration of important phytochemicals from pomegranate to a human or animal. More particularly, enables oral or enteral dosage forms containing phytochemicals from pomegranate for administering on a routine basis. In at least one instance the natural contents of a pomegranate are encapsulated into a pill form or concentrated juice that provides for more efficient administration than eating a pomegranate but contains the same key ingredients present in the fruit. Enables the recipient to receive the benefits of the fruit but allows for the dosages to be taken in pill or concentrated juice form. Composition is chemically similar or equal to a natural pomegranate but is in a powder, pill or concentrated liquid form.

Abstract: A method for treating waste material includes screening the material to produce “unders” that pass a screen and “overs” that do not. The overs are hydrothermally treated to produce a partially hydrolyzed biomass. The unders from the waste material, and the partially hydrolyzed biomass, are anaerobically digested. The method can also include recovering, and anaerobically digesting, volatile organic compounds from the hydrothermal treatment. A system for treating waste material comprises a screening device, an autoclave, and an anaerobic digester. The screening device separates the waste material into unders and overs, the autoclave receives the overs from the screening device and processes the overs with steam to produce a partially hydrolyzed biomass, and the digester receives and anaerobically digests the unders of the waste material and the partially hydrolyzed biomass. The system can also include an eductor coupled to the autoclave and configured to discharge to the digester.

Abstract: The present invention relates to the method for manufacturing textile, by treating textile with an isolated polypeptide having endoglucanase activity, especially in biostoning and bio-polishing process.